CN206486548U - The culture dish of umbilical cord mesenchymal stem cells - Google Patents
The culture dish of umbilical cord mesenchymal stem cells Download PDFInfo
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- CN206486548U CN206486548U CN201720028868.1U CN201720028868U CN206486548U CN 206486548 U CN206486548 U CN 206486548U CN 201720028868 U CN201720028868 U CN 201720028868U CN 206486548 U CN206486548 U CN 206486548U
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- culture plate
- umbilical cord
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Abstract
Covered the utility model discloses a kind of culture dish of umbilical cord mesenchymal stem cells, including on culture dish bottom, culture plate and culture dish, culture plate edge symmetric position is provided with 4 buckles；The culture plate edge is provided with liquid-changing hole between the buckle two-by-two；Multiple apertures are uniformly provided with the culture plate；The bottom of the culture plate is provided with four footing；It is conformed with the middle of the culture plate；The culture plate is fixed on the culture dish bottom by the buckle and footing.It is higher that the utility model tissue block goes out cell rate, and it is more to go out cell quantity, is convenient for changing nutrient solution, reduces cultivation cycle.
Description
Technical field
The utility model is related to culture dish, relates in particular to a kind of culture dish of umbilical cord mesenchymal stem cells.
Background technology
The separation of existing umbilical cord mesenchymal stem cells, which is mainly, uses tissue block adherent cultivation, is specifically by umbilical cord group
Surface sterilization sterilization is knitted, the blood vessel in umbilical cord and blood coagulation is removed, clean umbilical cord tissue is then shredded into 2-4mm2Left and right is big
Small, proper alignment is in Tissue Culture Flask or culture dish, and umbilical cord tissue block is firmly adhered on blake bottle when standing a period of time,
The nutrient solution for adding the volume of certain volume is statically placed in cell culture incubator.Because umbilical cord mesenchyma has the spy of adherent growth
Property, blake bottle grown on interior walls can be attached to from tissue block edge separate out.
The adherent process of umbilical cord mesenchymal stem cells generally requires to stand the time of one week or so, therefore is adding nutrient solution
When need to add enough nutrient solution.But umbilical cord tissue block, in enough nutrient solutions during quiescent culture, its edge is gradually
It can not gradually adhere well on blake bottle, or even meeting monoblock departs from blake bottle, floats in nutrient solution.This phenomenon is for the first time
Become apparent from when changing nutrient solution, most tissues block departs from blake bottle during all changing nutrient solution in first time.Umbilical cord tissue
Block is during adhere-wall culture, and only lower surface is contacted with blake bottle, and upper surface and surrounding can not be contacted with blake bottle, cause to connect
Contacting surface product is small.Tissue block adherent loosely with contact area is small has directly influenced the adherent of umbilical cord mesenchymal stem cells.The method
Shortcoming be exactly that to go out cell rate low for tissue block, it is few to go out cell quantity, and it is inconvenient to change nutrient solution, cultivation cycle length etc..
Utility model content
The technical problems to be solved in the utility model is to provide a kind of tissue block, and to go out cell rate higher, and it is more to go out cell quantity,
Nutrient solution is convenient for changing, the culture dish of the umbilical cord mesenchymal stem cells of cultivation cycle is reduced.
In order to solve the above-mentioned technical problem, the technical solution of the utility model is：A kind of training of umbilical cord mesenchymal stem cells
Ware is supported, including is covered on culture dish bottom, culture plate and culture dish, culture plate edge symmetric position is provided with 4 buckles；Institute
State culture plate edge and be provided with liquid-changing hole between the buckle two-by-two；Multiple apertures are uniformly provided with the culture plate；The culture
The bottom of plate is provided with four footing；It is conformed with the middle of the culture plate；The culture plate is fixed by the buckle and footing
On the culture dish bottom.
Preferably, the thickness of the culture plate is 1mm, a diameter of 8.5cm.
Preferably, the hole diameter is 2mm.
Preferably, distance is 5mm between the aperture and aperture.
Preferably, the culture plate and the gap 5mm at culture dish bottom.
Preferably, a height of 1cm of the projection, a width of 0.5cm, thickness are 0.2cm.
Preferably, the liquid-changing hole is semicircle, and radius is 0.5cm.
The beneficial effects of the utility model are：During the utility model use, by the way that umbilical cord tissue is stuck in into culture plate
Aperture in, can fix umbilical cord tissue block, and closely contacted with culture plate；Up and down the two of umbilical cord tissue block and culture plate
Face is contacted, and adds contact area；By liquid-changing hole, nutrient solution can be easily changed, and without worrying that umbilical cord tissue block can float
Float；This cultivating system convenient use microscope is observed, the adherent life on culture plate when umbilical cord mesenchymal stem cells
Grow to after sufficient amount, can be digested with pancreatin, collect digestive juice centrifugation, so that it may collect umbilical cord mesenchymal stem cells.Use buffer solution
Flushed culture plate can also continue to culture；By this continuous culture, more umbilical cord mesenchymal stem cells can be obtained.
Brief description of the drawings
Fig. 1 is lid on culture dish bottom, culture plate and the culture dish of the culture dish of the utility model umbilical cord mesenchymal stem cells
Structure explosion diagram；
Fig. 2 is the top view of the culture plate of the utility model umbilical cord mesenchymal stem cells；
Fig. 3 is Fig. 2 A-A sectional views.
Embodiment
Embodiment of the present utility model is described further below in conjunction with the accompanying drawings.Herein it should be noted that
Explanation for these embodiments is used to help understand the utility model, but does not constitute to restriction of the present utility model.This
Outside, as long as technical characteristic involved in each embodiment of the utility model disclosed below does not constitute conflict each other
It can just be mutually combined.
As shown in Figure 1 to Figure 3, a kind of culture dish of umbilical cord mesenchymal stem cells of the utility model, it includes culture dish bottom
1st, the part of lid 3 three on culture plate 2 and culture dish, wherein on culture dish bottom 1 (diameter 8.8cm) and culture dish lid 3 (diameter 9cm) and
Common Tissue Culture Dish is the same；Culture plate 2 is polystyrene, and material surface passes through the adherent process for treating surface of vacuum plasma
Processing；The thickness of culture plate 2 is a diameter of 8.5cm of 1mm；It is easy to use cooperatively with culture dish bottom 1；In the edge of culture plate 2
Symmetric position is provided with 4 buckles 21；Buckle 21 is set to tilt the face of culture plate 2；The bottom of culture plate 2 is provided with four footing 24；
Culture plate 2 is fixed on culture dish bottom 1 by buckle 21 and footing 24；Buckle 21 is specifically stuck in the oral area at culture dish bottom 1
Edge is pushed slightly toward culture dish bottom, and buckle 21 is fixed on the edge of culture dish bottom 1 by elastic deformation, 24 supports of footing
Act on and fix；The edge of culture plate 2 is provided with liquid-changing hole 22 between buckle 21 two-by-two；Liquid-changing hole 22 is semicircle, and radius is
0.5cm, conveniently changes nutrient solution；Multiple apertures 23 are uniformly provided with culture plate 2；The a diameter of 2mm of aperture 23；Aperture 23 and aperture 23
Between distance be 5mm；4 are conformed with the middle of culture plate 2；The height of projection 4 is that 1cm, a width of 0.5cm, thickness are 0.2cm, convenient
Lift monoblock culture plate.
What the utility model was specifically performed such, in use, clean umbilical cord tissue is shredded into 2-4mm2Left and right is big
It is small, in the aperture 23 that the tissue block shredded is filled in culture plate with syringe needle, tissue block is stuck in aperture 23 and from two
Emerge at end；After all apertures 23 are filled up, culture plate 2 is put into the culture plate of culture dish bottom 1 and culture dish bottom gap 5mm；Plus
Enter nutrient solution quiescent culture.
By the way that umbilical cord tissue is stuck in the aperture of culture plate, can fix umbilical cord tissue block, and closely with culture
Plate is contacted；The upper and lower surface contact of umbilical cord tissue block and culture plate, adds contact area；, can easily more by liquid-changing hole
Nutrient solution is changed, and without worrying that umbilical cord tissue block can float；This cultivating system convenient use microscope is observed, when
Umbilical cord mesenchymal stem cells adherent growth on culture plate, to after sufficient amount, can be digested with pancreatin, collect digestive juice centrifugation, just
Collect umbilical cord mesenchymal stem cells.The culture plate crossed with wash buffer can also continue to culture., can by this continuous culture
Obtain more umbilical cord mesenchymal stem cells.
Embodiment of the present utility model is explained in detail above in association with accompanying drawing, but the utility model is not limited to be retouched
The embodiment stated.For a person skilled in the art, it is right in the case where not departing from the utility model principle and spirit
These embodiments carry out a variety of change, modification, replacement and modification, still fall within protection domain of the present utility model.
Claims (7)
1. (3) are covered on a kind of culture dish of umbilical cord mesenchymal stem cells, including culture dish bottom (1), culture plate (2) and culture dish,
It is characterized in that：Culture plate (2) edge symmetric position is provided with 4 buckles (21)；Culture plate (2) edge is two-by-two
Liquid-changing hole (22) is provided between the buckle (21)；Multiple apertures (23) are uniformly provided with the culture plate (2)；The culture plate
(2) bottom is provided with four footing (24)；It is conformed with (4) in the middle of the culture plate (2)；The culture plate (2) is by described
Buckle (21) and footing (24) are fixed on the culture dish bottom (1).
2. the culture dish of umbilical cord mesenchymal stem cells according to claim 1, it is characterised in that：The thickness of the culture plate (2)
Spend for 1mm, a diameter of 8.5cm.
3. the culture dish of umbilical cord mesenchymal stem cells according to claim 1, it is characterised in that：The aperture (23) is a diameter of
2mm。
4. the culture dish of umbilical cord mesenchymal stem cells according to claim 1 or 3, it is characterised in that：The aperture (23) with
Distance is 5mm between aperture (23).
5. the culture dish of umbilical cord mesenchymal stem cells according to claim 1, it is characterised in that：The culture plate (2) and training
Support the gap 5mm of ware bottom (1).
6. the culture dish of umbilical cord mesenchymal stem cells according to claim 1, it is characterised in that：The projection (4) is a height of
1cm, a width of 0.5cm, thickness are 0.2cm.
7. the culture dish of umbilical cord mesenchymal stem cells according to claim 1, it is characterised in that：The liquid-changing hole (22) is half
Circle, radius is 0.5cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720028868.1U CN206486548U (en) | 2017-01-11 | 2017-01-11 | The culture dish of umbilical cord mesenchymal stem cells |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720028868.1U CN206486548U (en) | 2017-01-11 | 2017-01-11 | The culture dish of umbilical cord mesenchymal stem cells |
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| CN206486548U true CN206486548U (en) | 2017-09-12 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110257327A (en) * | 2019-06-27 | 2019-09-20 | 重庆市细胞生物工程技术有限公司 | A kind of isolated culture method of umbilical cord mesenchymal stem cells |
| WO2021008472A1 (en) * | 2019-07-15 | 2021-01-21 | 京东方科技集团股份有限公司 | Cell sheet transfer device and transport apparatus, and method for transferring cell sheet |
| CN112266875A (en) * | 2020-09-25 | 2021-01-26 | 生物岛实验室 | Cell culture container and method for culturing primary cells |
-
2017
- 2017-01-11 CN CN201720028868.1U patent/CN206486548U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110257327A (en) * | 2019-06-27 | 2019-09-20 | 重庆市细胞生物工程技术有限公司 | A kind of isolated culture method of umbilical cord mesenchymal stem cells |
| WO2021008472A1 (en) * | 2019-07-15 | 2021-01-21 | 京东方科技集团股份有限公司 | Cell sheet transfer device and transport apparatus, and method for transferring cell sheet |
| CN112266875A (en) * | 2020-09-25 | 2021-01-26 | 生物岛实验室 | Cell culture container and method for culturing primary cells |
| CN112266875B (en) * | 2020-09-25 | 2021-10-29 | 生物岛实验室 | Cell culture container and method for culturing primary cells |
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