CN113956977A - Multifunctional bath for in vitro electrophysiological recording - Google Patents
Multifunctional bath for in vitro electrophysiological recording Download PDFInfo
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- CN113956977A CN113956977A CN202111337519.5A CN202111337519A CN113956977A CN 113956977 A CN113956977 A CN 113956977A CN 202111337519 A CN202111337519 A CN 202111337519A CN 113956977 A CN113956977 A CN 113956977A
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- 238000000338 in vitro Methods 0.000 title claims abstract description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 119
- 239000000523 sample Substances 0.000 claims abstract description 38
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 23
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 23
- 239000001301 oxygen Substances 0.000 claims abstract description 23
- 238000010438 heat treatment Methods 0.000 claims abstract description 15
- 239000004575 stone Substances 0.000 claims description 11
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims description 8
- 229910002092 carbon dioxide Inorganic materials 0.000 claims description 4
- 239000001569 carbon dioxide Substances 0.000 claims description 4
- 239000007789 gas Substances 0.000 claims description 4
- 238000005086 pumping Methods 0.000 claims 1
- 238000011534 incubation Methods 0.000 abstract description 19
- 210000004556 brain Anatomy 0.000 abstract description 10
- 210000001175 cerebrospinal fluid Anatomy 0.000 abstract description 2
- 230000012447 hatching Effects 0.000 description 6
- 238000000034 method Methods 0.000 description 5
- 230000008569 process Effects 0.000 description 3
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 2
- 235000017491 Bambusa tulda Nutrition 0.000 description 2
- 241001330002 Bambuseae Species 0.000 description 2
- 241000699666 Mus <mouse, genus> Species 0.000 description 2
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 2
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 2
- 239000011425 bamboo Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 241000699670 Mus sp. Species 0.000 description 1
- 238000003491 array Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000005013 brain tissue Anatomy 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000005096 rolling process Methods 0.000 description 1
- 229940070527 tourmaline Drugs 0.000 description 1
- 229910052613 tourmaline Inorganic materials 0.000 description 1
- 239000011032 tourmaline Substances 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
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Abstract
The invention provides a multifunctional bath for in vitro electrophysiological recording, which relates to the technical field of incubation systems and comprises a box body, a water tank, a control box and a bath, wherein the water tank, the control box and the bath are sequentially connected in the box body, the bath realizes internal circulation with the water tank through a water inlet pipe and a water outlet pipe, and the control box is in control connection with a heating rod, an oxygen probe, a temperature probe and a PH probe in the water tank. The temperature, the PH and the oxygen of the artificial cerebrospinal fluid in the incubation groove are detected in real time through the temperature sensor, the PH sensor and the oxygen sensor, so that the experimental environment is detected in real time and is convenient to adjust. Compared with the traditional incubation system, the invention saves space, and compared with the traditional incubation system, the incubation system can incubate 6 mouse brain slices simultaneously, thereby improving the experimental efficiency.
Description
Technical Field
The invention relates to the technical field of incubation systems, in particular to a multifunctional bath for in-vitro electrophysiological recording.
Background
The traditional incubation system occupies a large area, only one bath tank of one incubation system can incubate the brain slices of the mice in the using process, the test efficiency is reduced, and resources are wasted. In the use process, the aCSF temperature needs to be artificially detected, so that the quality of the incubated brain slice is reduced, the real-time monitoring on the aCSF temperature, the PH value and the dissolved oxygen rate in the mouse brain slice incubation process cannot be met, and the related parameters are further adjusted in real time.
The patent document search of the prior art finds that the Chinese utility model has the patent publication number of CN206858581U, discloses an isolated brain slice incubation groove, belongs to the technical field of incubation grooves, ensures the survivability of cells, and makes the research of brain tissues better and more convenient. Including hatching groove main part, rotor plate, filter plate, hatching the ware and place the piece and hatch ware section of thick bamboo wall, hatching groove main part internally mounted and hatching the ware mounting panel, hatch ware mounting panel upper end and install hatching the ware, hatch the ware by hatching the ware and place the piece, hatch ware section of thick bamboo wall, door axis of rotation, rotating gate, door switch and hatch the ware main part and constitute, hatch groove main part one side lower extreme and install the inlet pipe, hatch groove main part opposite side lower extreme and install outlet pipe, inlet pipe and outlet pipe are constituteed by connecting pipe, toper honeycomb duct and filter plate, hatch groove main part top is installed and is hatched the groove upper cover, it comprises rotor plate, fixed plate, rolling bearing, switch installed part and switch to hatch the groove upper cover. The invention provides a small-sized multifunctional bath, which solves the problems of large occupied area and low test efficiency. Therefore, the method disclosed in the document and the invention belong to different inventive concepts.
Disclosure of Invention
In view of the drawbacks of the prior art, it is an object of the present invention to provide a multifunctional bath for in vitro electrophysiological recording.
The multifunctional bath for the in vitro electrophysiological recording comprises a box body, a water tank, a control box and a bath, wherein the water tank, the control box and the bath are sequentially connected in the box body, the bath realizes internal circulation with the water tank through a water inlet pipe and a water outlet pipe, and the control box is in control connection with a heating rod, an oxygen probe, a temperature probe and a PH probe in the water tank.
Preferably, the bath is provided with a water inlet and a water outlet, the water inlet is connected to the water inlet pipe through a three-way water inlet switch, the water inlet pipe extends to the water tank, the water outlet is connected with the water outlet pipe, and the water outlet pipe is connected to the water tank.
Preferably, the water inlet pipe is connected with a micro water pump, the micro water pump is arranged in the water tank, and the micro water pump is in control connection with the three-way water inlet switch through a control box;
the control box respectively opens the three-way water inlet switch and the micro water pump, the water inlet pipe inputs aCSF in the water tank into the bath through the micro water pump, and when the aCSF in the bath is higher than the water outlet, the aCSF flows back to the water tank through the water outlet pipe.
Preferably, the device further comprises an air stone, wherein the air stone is arranged in the water tank, and the air stone pumps out 95% of oxygen and 5% of carbon dioxide to provide oxygen for aCSF in the water tank.
Preferably, the control box includes comprehensive control district and LED display, and the LED display is connected in the comprehensive control district, and the comprehensive control district gathers the detection numerical value of oxygen probe, temperature probe and PH probe to show through the LED display.
Preferably, when the temperature probe detects that the temperature in the water tank is too low, the control box starts the heating rod; when the temperature probe detects that the temperature in the water tank reaches the preset temperature, the control box closes the heating rod.
Preferably, the bath comprises a bath body and gauze, the gauze is connected to the inner wall of the bath body, and the height of the gauze is lower than that of the water outlet.
Preferably, the height of the water outlet is lower than the height of the water inlet.
Preferably, a plurality of bath arrays are arranged in the housing.
Preferably, the number of baths is one.
Compared with the prior art, the invention has the following beneficial effects:
(1) the invention integrates the membrane technology to incubate a set of flow in vitro, and reduces the volume of an incubation system under the condition of keeping all functions complete, thereby saving the space of an electrophysiological laboratory;
(2) the invention is provided with 6 circular incubation grooves at the same time, each circular bath groove is provided with an independent three-way switch, and one or more bath grooves can be independently used, thereby greatly improving the working efficiency;
(3) the invention solves the aim of detecting aCSF in real time by installing an oxygen probe, a temperature probe and a PH probe, thereby ensuring that the electrophysiological technology is more accurate and stable.
(4) The invention is provided with the temperature sensor and the heating rod, and can fully stabilize the temperature of aCSF solution, thereby enabling electrophysiological recording to be more stable.
Drawings
Other features, objects and advantages of the invention will become more apparent upon reading of the detailed description of non-limiting embodiments with reference to the following drawings:
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic view of a bath configuration of the present invention;
fig. 3 is a schematic structural diagram of the control box of the present invention.
Reference numbers in the figures:
the device comprises a box body 100, a bath 1, a gas stone 7, a water tank 8, a micro water pump 9, a heating rod 10, an oxygen probe 11, a temperature probe 12, a water inlet pipe 13, a control box 14, a comprehensive control area 141, an LED display 142, gauze 15, a water inlet 16, a water outlet 17, a three-way water inlet switch 18 and a PH probe 19.
Detailed Description
The present invention will be described in detail with reference to specific examples. The following examples will assist those skilled in the art in further understanding the invention, but are not intended to limit the invention in any way. It should be noted that it would be obvious to those skilled in the art that various changes and modifications can be made without departing from the spirit of the invention. All falling within the scope of the present invention.
Example 1
The multifunctional bath for the in vitro electrophysiological recording comprises a box body 100, a water tank 8, a control box 14, a tourmaline 7 and a bath 1, wherein the water tank 8, the control box 9 and the bath 1 are sequentially connected in the box body 100, the bath 1 realizes internal circulation with the water tank 8 through a water inlet pipe 13 and a water outlet pipe 19, and the control box 14 is in control connection with a heating rod 10, an oxygen probe 11, a temperature probe 12 and a PH probe 19 in the water tank 8. The air stone 7 is placed in the water tank 8, and the air stone 7 pumps out 95% of oxygen and 5% of carbon dioxide to provide oxygen for aCSF in the water tank 8.
A water inlet 16 and a water outlet 17 are arranged on the bath 1, the water inlet 16 is connected to a water inlet pipe 13 through a three-way water inlet switch 18, the water inlet pipe 13 extends to the water tank 8, the water outlet 17 is connected with a water outlet pipe 19, and the water outlet pipe 19 is connected to the water tank 8. The water inlet pipe 13 is connected with the micro water pump 9, and the micro water pump 9 is arranged in the water tank 8. A micro water pump powers the aCSF circulation. The micro water pump 9 is connected with the three-way water inlet switch 18 through the control box 14. The bath 1 comprises a tank body 111 and gauze 15, the gauze 15 is connected to the inner wall of the tank body 111, the height of the gauze 15 is lower than that of the water outlet 17, and the height of the water outlet 17 is lower than that of the water inlet 16.
The control box 14 comprises an integrated control area 141 and an LED display 142, the integrated control area 141 is connected with the LED display 142, and the integrated control area 141 visually displays data fed back by the oxygen probe 11, the temperature probe 12 and the PH probe 19 through the LED display.
More specifically, the main function of the air stone 7 is to provide oxygen for aCSF in the water tank 8, and the air pumped out by the air stone is a mixed air containing 95% of oxygen and 5% of carbon dioxide, so as to ensure the normal activity of brain slices; the brain slice is required to be incubated at a fixed temperature, the activity of the brain slice and the stability of electrophysiological recording are influenced by temperature change of the brain slice, the heating pump is connected with the control box 14, once the temperature probe 12 detects that the temperature of aCSF in the water tank 8 deviates from a set temperature, the heating pump 10 is started to heat the aCSF to the set temperature, and once the temperature sensor 12 detects that the temperature reaches the set temperature, the power supply of the heating pump 10 is disconnected, so that a fixed temperature regulation and control loop is formed, and the incubation of the aCSF is ensured at the set constant temperature; and the PH probe 19 can detect the PH in the water tank 8 in real time and provides a basis for timely adjusting the PH.
The working principle is as follows:the comprehensive control area 141 respectively opens the three-way water inlet switch 18 and the micro water pump 9, the water inlet pipe 13 inputs aCSF in the water tank 8 into the bath 1 through the micro water pump 9, and when the aCSF in the bath 1 is higher than the water outlet 17, the aCSF flows back to the water tank 8 through the water outlet pipe 19. When the temperature probe 12 detects that the temperature in the water tank 8 is too low, the integrated control area 141 starts the heating rod 10; when the temperature probe 12 detects that the temperature in the water tank 8 reaches the preset temperature, the integrated control section 141 turns off the heating rod 10. The integrated control area 141 collects the detected values of the oxygen probe 11, the temperature probe 12 and the PH probe 19, and displays the detected values through the LED display 142.
The temperature, the PH and the oxygen of the artificial cerebrospinal fluid in the incubation groove are detected in real time through the temperature sensor, the PH sensor and the oxygen sensor, so that the experimental environment is detected in real time and is convenient to adjust. Compared with the traditional incubation system, the invention saves space, and compared with the traditional incubation system, the incubation system can incubate 6 mouse brain slices simultaneously, thereby improving the experimental efficiency.
Example 2
The embodiment 2 is completed on the basis of the embodiment 1, the volume of the incubation system is reduced under the condition of keeping the integrity of each function, so that the space of an electrophysiological laboratory is saved, and the working efficiency is improved by using a plurality of baths. Specifically, the method comprises the following steps:
the box body 100 is composed of a transparent white acrylic block with the length of 20cm, the width of 15cm and the height of 15cm, 6 baths are contained in the box body 100, the 6 baths 1 are arranged in an array, and the diameter of each bath 1 is 4 cm. The water outlet 17 in the bath 1 is 3cm below the water inlet 16. The water tank 8 is a transparent acrylic water tank with the length of 12cm, the width of 9cm and the height of 12cm, and mainly has the function of containing aCSF.
In the description of the present application, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience in describing the present application and simplifying the description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present application.
The foregoing description of specific embodiments of the present invention has been presented. It is to be understood that the present invention is not limited to the specific embodiments described above, and that various changes or modifications may be made by one skilled in the art within the scope of the appended claims without departing from the spirit of the invention. The embodiments and features of the embodiments of the present application may be combined with each other arbitrarily without conflict.
Claims (10)
1. The utility model provides a multi-functional bath for external electrophysiological recording, characterized in that, includes box (100), basin (8), control box (14) and bath (1), has connected gradually in box (100) basin (8), control box (9) and bath (1), bath (1) through inlet tube (13) and outlet pipe (19) with basin (8) realize the inner loop, control box (14) control connection heating rod (10), oxygen probe (11), temperature probe (12) and PH probe (19) in basin (8).
2. The multifunctional bath for in vitro electrophysiological recording of claim 1, wherein the bath (1) is provided with a water inlet (16) and a water outlet (17), the water inlet (16) is connected to the water inlet tube (13) by a three-way water inlet switch (18), the water inlet tube (13) extends to the basin (8), the water outlet (17) is connected to the water outlet tube (19), and the water outlet tube (19) is connected to the basin (8).
3. The multifunctional bath for in vitro electrophysiological recording of claim 2, characterized in that the water inlet pipe (13) is connected to a micro water pump (9), the micro water pump (9) is placed in the water tank (8), and the micro water pump (9) and the three-way water inlet switch (18) are in control connection through the control box (14);
the control box (9) is opened respectively tee bend water inlet switch (18) and miniature pump (9), inlet tube (13) will through miniature pump (9) artifical cerebrospinal fluid-aCSF input in basin (8) in the bath (1), when aCSF position in the bath (1) is higher than when delivery port (17), through outlet pipe (19) backward flow to in the basin (8).
4. The multifunctional bath for in vitro electrophysiological recording of claim 3, further comprising a gas stone (7), the gas stone (7) being placed inside the water tank (8), the gas stone (7) pumping 95% oxygen and 5% carbon dioxide to provide oxygen for aCSF in the water tank (8).
5. The multifunctional bath for in vitro electrophysiological recording of claim 4, characterized in that the control box (14) comprises an integrated control area (141) and an LED display (142), wherein the integrated control area (141) is connected to the LED display (142), and the integrated control area (141) collects the detected values of the oxygen probe (11), the temperature probe (12) and the PH probe (19) and displays the detected values through the LED display (142).
6. Multifunctional bath for in vitro electrophysiological recording according to claim 5, characterized in that the control box (14) turns on the heating rod (10) when the temperature probe (12) detects that the temperature in the water tank (8) is too low; when the temperature probe (12) detects that the temperature in the water tank (8) reaches a preset temperature, the control box (14) closes the heating rod (10).
7. The multifunctional bath for in vitro electrophysiological recording of claim 6, characterized in that the bath (1) comprises a basin (111) and gauze (15), the gauze (15) being connected to the inner wall of the basin (111), the gauze (15) having a height lower than the height of the water outlet (17).
8. Multifunctional bath for in vitro electrophysiological recording according to claim 7, characterized in that the water outlet (17) has a lower height than the water inlet (16).
9. Multifunctional bath for in vitro electrophysiological recording according to claim 8, characterized in that a plurality of baths (1) are arranged in an array in the tank (100).
10. Multifunctional bath for in vitro electrophysiological recording according to claim 9, characterized in that the number of baths (1) is 6.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN202111337519.5A CN113956977A (en) | 2021-11-09 | 2021-11-09 | Multifunctional bath for in vitro electrophysiological recording |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202111337519.5A CN113956977A (en) | 2021-11-09 | 2021-11-09 | Multifunctional bath for in vitro electrophysiological recording |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114252324A (en) * | 2022-03-02 | 2022-03-29 | 中国人民解放军军事科学院军事医学研究院 | Device is hatched to porous brain piece that facilitates use |
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| US6048722A (en) * | 1995-11-08 | 2000-04-11 | Trustees Of Boston University | Cellular physiology workstations for automated data acquisition and perfusion control |
| CN204111732U (en) * | 2014-07-10 | 2015-01-21 | 中国人民解放军第四军医大学 | The diaphragm incubating device that a kind of temperature, dissolved oxygen are controlled |
| CN206467243U (en) * | 2017-02-15 | 2017-09-05 | 南京市第一医院 | A kind of patch-clamp cell record groove thermostat |
| CN207882277U (en) * | 2018-03-19 | 2018-09-18 | 天晴干细胞股份有限公司 | A kind of incubation moisture preservation box for immunological experiment |
| CN109749929A (en) * | 2019-02-22 | 2019-05-14 | 中国人民解放军第四军医大学 | A kind of brain slice in vitro rewarming incubating device of real-time monitoring state |
| US20210139828A1 (en) * | 2019-11-07 | 2021-05-13 | Lawrence Livermore National Security, Llc | System and method for three-dimensional in vitro flexible microelectrode array |
-
2021
- 2021-11-09 CN CN202111337519.5A patent/CN113956977A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6048722A (en) * | 1995-11-08 | 2000-04-11 | Trustees Of Boston University | Cellular physiology workstations for automated data acquisition and perfusion control |
| CN204111732U (en) * | 2014-07-10 | 2015-01-21 | 中国人民解放军第四军医大学 | The diaphragm incubating device that a kind of temperature, dissolved oxygen are controlled |
| CN206467243U (en) * | 2017-02-15 | 2017-09-05 | 南京市第一医院 | A kind of patch-clamp cell record groove thermostat |
| CN207882277U (en) * | 2018-03-19 | 2018-09-18 | 天晴干细胞股份有限公司 | A kind of incubation moisture preservation box for immunological experiment |
| CN109749929A (en) * | 2019-02-22 | 2019-05-14 | 中国人民解放军第四军医大学 | A kind of brain slice in vitro rewarming incubating device of real-time monitoring state |
| US20210139828A1 (en) * | 2019-11-07 | 2021-05-13 | Lawrence Livermore National Security, Llc | System and method for three-dimensional in vitro flexible microelectrode array |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114252324A (en) * | 2022-03-02 | 2022-03-29 | 中国人民解放军军事科学院军事医学研究院 | Device is hatched to porous brain piece that facilitates use |
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Application publication date: 20220121 |
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