CN114167042A - Multifunctional platform for patch clamp recording and use method thereof - Google Patents
Multifunctional platform for patch clamp recording and use method thereof Download PDFInfo
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- 238000000034 method Methods 0.000 title claims abstract description 11
- 239000000523 sample Substances 0.000 claims abstract description 57
- 210000004556 brain Anatomy 0.000 claims abstract description 53
- 239000007788 liquid Substances 0.000 claims abstract description 29
- 230000002572 peristaltic effect Effects 0.000 claims description 21
- 230000000638 stimulation Effects 0.000 claims description 19
- 238000012377 drug delivery Methods 0.000 claims description 8
- 238000002347 injection Methods 0.000 claims description 8
- 239000007924 injection Substances 0.000 claims description 8
- 238000001514 detection method Methods 0.000 claims description 7
- 239000000243 solution Substances 0.000 claims description 7
- 238000010438 heat treatment Methods 0.000 claims description 6
- 230000004936 stimulating effect Effects 0.000 claims description 5
- 125000004122 cyclic group Chemical group 0.000 claims description 2
- 240000007594 Oryza sativa Species 0.000 claims 1
- 235000007164 Oryza sativa Nutrition 0.000 claims 1
- 235000009566 rice Nutrition 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 4
- 239000006185 dispersion Substances 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 description 23
- 239000012528 membrane Substances 0.000 description 9
- 230000006870 function Effects 0.000 description 8
- 239000003814 drug Substances 0.000 description 6
- 229940079593 drug Drugs 0.000 description 5
- 210000003722 extracellular fluid Anatomy 0.000 description 5
- 102000004310 Ion Channels Human genes 0.000 description 3
- 210000001175 cerebrospinal fluid Anatomy 0.000 description 3
- 229920001971 elastomer Polymers 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 210000002569 neuron Anatomy 0.000 description 3
- 230000010412 perfusion Effects 0.000 description 3
- 239000004020 conductor Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
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- 238000012544 monitoring process Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
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- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000010259 detection of temperature stimulus Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000001647 drug administration Methods 0.000 description 1
- 238000004070 electrodeposition Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 238000011897 real-time detection Methods 0.000 description 1
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- 210000001519 tissue Anatomy 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/4833—Physical analysis of biological material of solid biological material, e.g. tissue samples, cell cultures
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Abstract
The invention provides a multifunctional platform for patch clamp recording and a using method thereof, wherein the multifunctional platform comprises a recording platform, wherein a liquid storage tank, a brain slice placing groove and a probe placing groove are arranged on the recording platform; the liquid storage tank is communicated with the brain slice placing groove; the brain slice placing groove and the probe placing groove are communicated with each other. The original dispersion function is organically and reasonably integrated into the recording bath, so that the problems of wiring of the recording bath and orderly arrangement of all devices are solved, the recording noise is reduced, and the space is saved; a probe placing groove is additionally arranged, so that the problem that the recording is influenced by the random placement of the grounding electrode is solved, and the problem that the recording effect is influenced by the random placement and swing of the reference electrode is solved; the pH and temperature of aCSF can be monitored by organically adding a temperature sensor and a pH sensor, so that the state of aCSF can be monitored in real time.
Description
Technical Field
The invention relates to the technical field of multifunctional platforms, in particular to a multifunctional platform for patch clamp recording and a using method thereof.
Background
The patch clamp is also called single-channel current recording technology, a specially-made glass micropipette is adsorbed on the surface of a cell to form 10-100 of seal (giga-seal), also called giant resistance seal, the area of an isolated small membrane is in the order of mum, and only a few ion channels exist in the small membrane. Voltage clamping was then applied to the membrane and currents of the order of pA (negative 12 square amperes at 10) generated by the opening of a single ion channel, a random process, could be measured. By observing the current change of opening and closing of a single channel, functional parameters such as current amplitude distribution, opening probability, opening service life distribution and the like of opening of various ion channels can be directly obtained, and the relationship between the functional parameters and membrane potential, ion concentration and the like is analyzed. The membrane absorbed by the suction tube can be separated from the cell membrane, and the experimental study can be carried out in a mode that the outer side of the membrane faces outwards or the inner side of the membrane faces outwards. This technique is convenient for voltage clamping of the minicells, changing the composition of the intra-membrane and extra-membrane solutions, and applying drugs.
The cell recording groove is designed and manufactured in order to match with the development of the patch clamp technology, and the cell recording groove is used for continuously keeping a place which survives for a long time after the acute separated living cells, cultured cells, living instantaneous tissue slices and the like are placed in the cell recording groove, so that the process of clamping the cells by the patch clamp is smoothly finished. The cell recording groove is provided with a place for exchanging extracellular fluid for keeping living cells alive for a long time, the exchange is to inject extracellular fluid which meets different requirements and is saturated by oxygen into the living cells or slices in the cell recording groove by an auxiliary medicine adding device, or inject extracellular fluid mixed with drug components with different concentrations, after the cells are fully acted, the excessive extracellular fluid in the cell recording groove is sucked out by an auxiliary vacuum pump, but a certain amount of extracellular fluid is reserved in the recording groove, so that the cells or slices are deposited at the bottom of the cell recording groove and are not washed away by perfusion fluid, and an experimenter can conveniently carry out patch clamp experiments on the cells or the slices. The cell recording groove also can be a heat transfer conductor which is suitable for an attached heating system to heat and preserve the temperature of the recording groove and maintain the physiological requirements of living cells in the cell recording groove.
Chinese patent publication No. CN108490165A discloses a constant temperature patch clamp cell recording system, which includes a cell console, a cell recording tank disposed on the cell console, a perfusion device connected to the cell recording tank, a constant temperature dish cooperating with the cell recording tank, a constant temperature column disposed at least on the perfusion device, and a constant temperature machine connected to the constant temperature column and the constant temperature dish.
With respect to the related art in the above, the inventor considers that the platform has less functions and is not reasonable in use.
Disclosure of Invention
In view of the defects in the prior art, the invention aims to provide a multifunctional platform for patch clamp recording and a using method thereof.
The multifunctional platform for patch clamp recording comprises a recording platform, wherein a liquid storage tank, a brain slice placing groove and a probe placing groove are arranged on the recording platform;
the liquid storage tank is communicated with the brain slice placing groove;
the brain slice placing groove and the probe placing groove are communicated with each other.
Preferably, the multifunctional platform also comprises a circulating power system which enables the liquid storage tank, the brain slice placing groove and the probe placing groove to form circulating circulation;
the circulating power system comprises a peristaltic pump, a first connecting pipe, a second connecting pipe, a third connecting pipe and a containing cup;
one end of the first connecting pipe is communicated with the liquid storage tank, and the other end of the first connecting pipe is communicated with the peristaltic pump;
one end of the second connecting pipe is communicated with the peristaltic pump, and the other end of the second connecting pipe is communicated with the containing cup;
one end of the third connecting pipe is communicated with the containing cup, and the other end of the third connecting pipe is communicated with the probe placing groove;
the liquid storage tank, the first connecting pipe, the peristaltic pump, the second connecting pipe, the containing cup, the third connecting pipe, the probe placing groove and the brain slice placing groove form a closed circulating system.
Preferably, the first connecting pipe is sleeved with a heating sleeve.
Preferably, the multifunctional platform further comprises a drug delivery system and a micro-manipulator, wherein the drug delivery system comprises a pressure system and an injection electrode;
the injection electrode is adjusted to the brain slice administration position in the brain slice placing groove through a micromanipulation, and administration is carried out through a pressure system.
Preferably, the multi-functional platform further comprises a stimulation system comprising a stimulator and a stimulation electrode; the stimulation electrode is disposed on the micro-manipulator;
the position of the stimulating electrode is adjusted through the micromanipulation, and different positions of the brain slices in the brain slice placing groove are electrically stimulated through the stimulator.
Preferably, the multifunctional platform further comprises a detection system, wherein the detection system comprises a temperature sensor, a pH sensor and a control box;
the temperature sensor and the pH sensor are both connected with the control box; and the probe of the temperature sensor and the probe of the pH sensor are placed in the probe placing groove.
Preferably, the temperature sensor and the pH sensor are respectively connected with the control box through leads.
Preferably, the multi-functional platform further comprises a ground electrode, the ground electrode being located on the probe placement groove.
Preferably, the liquid storage tank and the brain slice placing tank are communicated with each other through a first communicating pipe;
the brain slice placing groove and the probe placing groove are communicated with each other through a second communicating pipe.
The invention provides a method for using a multifunctional platform for patch clamp recording, which comprises the following cyclic steps: the peristaltic pump circulates the solution in the liquid storage tank, the first connecting pipe, the peristaltic pump, the second connecting pipe, the containing cup, the third connecting pipe, the probe placing groove and the brain slice placing groove.
Compared with the prior art, the invention has the following beneficial effects:
1. the original dispersion function is organically and reasonably integrated into the recording bath, so that the problems of wiring of the recording bath and orderly arrangement of all devices are solved, the recording noise is reduced, and the space is saved;
2. the invention adds a probe placing groove, solves the problem that the arbitrary placement of the grounding electrode influences the recording, thereby solving the problem that the arbitrary placement and swing of the reference electrode influences the recording effect;
3. the invention can monitor the pH and temperature of aCSF by organically adding a temperature sensor and a pH sensor, thereby monitoring the state of aCSF in real time.
Drawings
Other features, objects and advantages of the invention will become more apparent upon reading of the detailed description of non-limiting embodiments with reference to the following drawings:
fig. 1 is a schematic view of the overall structure of the embodiment of the present invention.
Reference numerals:
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| Brain |
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| Probe placing groove 4 | Second communicating pipe 11 | |
| Temperature sensor 5 | |
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| pH sensor 6 | |
Micro-operation 20 |
| Reference electrode 7 | PH and temperature display and |
Detailed Description
The present invention will be described in detail with reference to specific examples. The following examples will assist those skilled in the art in further understanding the invention, but are not intended to limit the invention in any way. It should be noted that it would be obvious to those skilled in the art that various changes and modifications can be made without departing from the spirit of the invention. All falling within the scope of the present invention.
The embodiment of the invention discloses a multifunctional platform for patch clamp recording, which comprises a recording platform 1, a circulating power system, a drug delivery system, a micro-manipulator 20, a stimulation system, a detection system and a grounding electrode, and is shown in figure 1. The ground electrode comprises a reference electrode 7.
The recording platform 1 is provided with a liquid storage tank 2, a brain slice placing groove 3 and a probe placing groove 4. The liquid storage tank 2 and the brain slice placing tank 3 are communicated with each other. The brain slice placing groove 3 and the probe placing groove 4 are communicated with each other. The liquid storage tank 2 and the brain slice placing tank 3 are communicated with each other through a first communicating pipe 10; the brain slice placing groove 3 and the probe placing groove 4 are communicated with each other through a second communicating pipe 11. The recording platform 1 comprises a recording groove, and the brain slice placing groove 3 comprises a circular brain slice placing groove 3. The subject of the invention is a recording groove consisting of a transparent square acrylic sheet, and the subject of the recording groove comprises three parts: a liquid storage tank 2 which has the main function of temporarily storing aCSF; the circular brain slice placing groove 3 is mainly used for placing recorded brain slices; the probe placing groove 4 mainly has the functions of placing a pH probe, a temperature sensor 5 probe and a reference electrode 7, the liquid storage groove 2 is communicated with the brain slice placing groove 3 through a first communicating pipe 10, the brain slice placing groove 3 is communicated with the probe placing groove 4 through a second communicating pipe 11, and the fact that aCSF can be communicated with each other in the liquid storage groove 2-the brain slice placing groove 3-the probe placing groove 4 is achieved. aCSF is generally called as Artificial Cerebrospinal Fluid in English, and the Chinese translation is Artificial Cerebrospinal Fluid. The sizes of the recording tank, the liquid storage tank 2 and the circular brain slice placing tank 3 can be adjusted according to personal preference and experimental requirements.
The circulation power system enables the liquid storage tank 2, the brain slice placing tank 3 and the probe placing tank 4 to form circulation. The circulating power system comprises a peristaltic pump 15, a first connecting pipe, a second connecting pipe, a third connecting pipe and a containing cup. One end of the first connecting pipe is communicated with the liquid storage tank 2, and the other end of the first connecting pipe is communicated with the peristaltic pump 15. One end of the second connecting pipe is communicated with the peristaltic pump 15, and the other end of the second connecting pipe is communicated with the containing cup. One end of the third connecting pipe is communicated with the containing cup, and the other end of the third connecting pipe is communicated with the probe placing groove 4; the liquid storage tank 2, the first connecting pipe, the peristaltic pump 15, the second connecting pipe, the containing cup, the third connecting pipe, the probe placing groove 4 and the brain slice placing groove 3 form a closed circulating system. The first connecting pipe, the second connecting pipe and the third connecting pipe each include a rubber pipe 17. The holding cup comprises an aCSF beaker 19. The power system is provided by a peristaltic pump 15, and the solution in the aCSF beaker 19 is pumped into the liquid storage tank 2 through a rubber tube 17 and flows out of the sensor placement groove (the probe placement groove 4) to the aCSF beaker 19, so that a closed circulation system is formed. The first connecting pipe is sleeved with a heating sleeve 9. The solution in the aCSF beaker 19 comprises aCSF and the solution flows in a counter-clockwise direction in a closed loop system, the effect of the one-way flow being to allow the aCSF to reach the reservoir 2 first.
The drug delivery system comprises a pressure system 12 and an injection electrode; the injection electrode is adjusted to the brain slice administration position in the brain slice placing groove 3 by the micromanipulator 20, and the administration is carried out by the pressure system 12. The stimulation system comprises a stimulator 13 and a stimulation electrode 18; stimulation electrodes 18 are disposed on the micro-manipulator 20; the position of the stimulating electrode 18 is adjusted through the micromanipulator 20, and different positions of the brain slices in the brain slice placing groove 3 are electrically stimulated through the stimulator 13. The study of the effect of drugs on the characteristics and functions of neurons in electrophysiological studies is a very common experiment, and the injection electrodes are adjusted to the administration position of brain tablets by a micromanipulator 20, so that the drugs can be accurately administered through a drug administration system. Meanwhile, the multifunctional platform is also provided with a stimulation system, the stimulation electrode 18 is arranged on the micromanipulation 20, and the position of the stimulation electrode 18 is adjusted through the micromanipulation 20, so that the electrical stimulation is carried out on different positions of the brain slice. The injection electrode comprises a dosing electrode 8.
The detection system comprises a temperature and pH detection system, and the state of the brain slice aCSF is monitored and recorded in real time. The temperature and pH detection system comprises a temperature sensor 5, a pH sensor 6 and a control box. The control box comprises a pH and temperature display and control box 14, and the pH and temperature display and control box 14 comprises an LED display screen. The temperature sensor 5 and the pH sensor 6 are both connected with a control box; the probe of the temperature sensor 5 and the probe of the pH sensor 6 are placed in the probe placement groove 4. The temperature sensor 5 and the pH sensor 6 are respectively connected with the control box through leads 16. The probe of the pH sensor 6 comprises a pH micro sensing probe, the probe of the temperature sensor 5 comprises a micro temperature sensing probe, the pH micro sensing probe and the micro temperature sensing probe are connected to the control box through a lead 16, and the current temperature and the pH are displayed in real time through an led display screen. The ground electrode is located on the probe placement groove 4. The invention is also specially provided with a grounding electrode position which is positioned in the probe placing groove 4, so that the grounding electrode can be conveniently placed. The heating jacket 9 is connected with the control box. The reference electrode 7 is placed at a position in the probe placement groove 4 alone, and the probe of the temperature sensor 5 and the probe of the ph sensor 6 are placed at a position in the probe placement groove 4 alone, so that mutual interference is avoided.
In conclusion, the multifunctional recording tank can detect pH and temperature in real time and has the stimulation function of the administration electrode 8. The temperature probe and the pH probe are utilized to detect and record the temperature of the artificial cerebrospinal fluid aCSF in the groove in real time, and the pressure system 12 can adjust the temperature of the aCSF in real time. The liquid inlet speed can be adjusted in real time through the peristaltic pump 15, and the stimulating electrode 18 can stimulate the brain slice. In conclusion, the recording table realizes real-time detection of temperature and pH; meanwhile, the real-time regulation and control of the temperature and the liquid inlet speed are realized; if the experiment needs the stimulating electrode 18 to stimulate the brain slice. The closed circulation system and the monitoring system are both required to be opened as long as the system runs, the stimulation system and the drug delivery system are required according to experimental requirements, when the neuron function needs to be explored through electrical stimulation, the stimulation system is required, and when the influence of drugs on the neuron function or the brain function needs to be researched, the drug delivery system is required.
The embodiment of the invention also discloses a use method of the multifunctional platform for patch clamp recording, which comprises the following circulating steps: the peristaltic pump 15 circulates the solution in the liquid storage tank 2, the first connecting pipe, the peristaltic pump 15, the second connecting pipe, the containing cup, the third connecting pipe, the probe placing groove 4 and the brain slice placing groove 3.
In the description of the present application, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience in describing the present application and simplifying the description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present application.
The foregoing description of specific embodiments of the present invention has been presented. It is to be understood that the present invention is not limited to the specific embodiments described above, and that various changes or modifications may be made by one skilled in the art within the scope of the appended claims without departing from the spirit of the invention. The embodiments and features of the embodiments of the present application may be combined with each other arbitrarily without conflict.
Claims (10)
1. The multifunctional platform for patch clamp recording is characterized by comprising a recording platform (1), wherein a liquid storage tank (2), a brain slice placing groove (3) and a probe placing groove (4) are arranged on the recording platform (1);
the liquid storage tank (2) is communicated with the brain slice placing tank (3);
the brain slice placing groove (3) and the probe placing groove (4) are communicated with each other.
2. The multi-functional platform for patch-clamp recordings according to claim 1, characterized in that it further comprises a circulation power system that circulates the reservoir (2), the brain slice placement tank (3) and the probe placement tank (4);
the circulating power system comprises a peristaltic pump (15), a first connecting pipe, a second connecting pipe, a third connecting pipe and a containing cup;
one end of the first connecting pipe is communicated with the liquid storage tank (2), and the other end of the first connecting pipe is communicated with the peristaltic pump (15);
one end of the second connecting pipe is communicated with the peristaltic pump (15), and the other end of the second connecting pipe is communicated with the containing cup;
one end of the third connecting pipe is communicated with the containing cup, and the other end of the third connecting pipe is communicated with the probe placing groove (4);
the liquid storage tank (2), the first connecting pipe, the peristaltic pump (15), the second connecting pipe, the rice containing cup, the third connecting pipe, the probe placing groove (4) and the brain slice placing groove (3) form a closed circulating system.
3. The multifunctional platform for patch-clamp recording according to claim 2, characterized in that the first connecting tube is sleeved with a heating jacket (9).
4. The multi-functional platform for patch-clamp recording according to claim 1, further comprising a drug delivery system comprising a pressure system (12) and an injection electrode, and a micromanipulator (20);
the injection electrode is adjusted to the brain slice administration position in the brain slice placing groove (3) through a micromanipulator (20), and administration is carried out through the pressure system (12).
5. The multi-functional platform for patch-clamp recording according to claim 4, further comprising a stimulation system comprising a stimulator (13) and a stimulation electrode (18); the stimulation electrode (18) is disposed on a micromanipulation (20);
the position of the stimulating electrode (18) is adjusted through the micromanipulation (20), and different positions of the brain slices in the brain slice placing groove (3) are electrically stimulated through the stimulator (13).
6. The multifunctional platform for patch-clamp recording according to claim 1, further comprising a detection system comprising a temperature sensor (5), a pH sensor (6) and a control box;
the temperature sensor (5) and the pH sensor (6) are both connected with a control box; the probe of the temperature sensor (5) and the probe of the pH sensor (6) are placed in the probe placing groove (4).
7. The multifunctional platform for patch clamp recording according to claim 6, wherein the temperature sensor (5) and the pH sensor (6) are connected to a control box through wires (16), respectively.
8. Multifunctional platform for patch-clamp recording according to claim 7, characterized in that it further comprises a ground electrode, which is located at the probe placement groove (4).
9. The multifunctional platform for patch-clamp recording according to claim 1, wherein the reservoir (2) and the brain slice placement tank (3) are communicated with each other through a first communication pipe (10);
the brain slice placing groove (3) and the probe placing groove (4) are communicated with each other through a second communicating pipe (11).
10. A method of using a multifunctional platform for patch clamp recordings, using the multifunctional platform for patch clamp recordings according to any one of claims 1 to 9, comprising the cyclic steps of: the peristaltic pump (15) enables the solution in the liquid storage tank (2), the first connecting pipe, the peristaltic pump (15), the second connecting pipe, the containing cup, the third connecting pipe, the probe placing groove (4) and the brain slice placing groove (3) to circulate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN202111364203.5A CN114167042A (en) | 2021-11-17 | 2021-11-17 | Multifunctional platform for patch clamp recording and use method thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202111364203.5A CN114167042A (en) | 2021-11-17 | 2021-11-17 | Multifunctional platform for patch clamp recording and use method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN204111732U (en) * | 2014-07-10 | 2015-01-21 | 中国人民解放军第四军医大学 | The diaphragm incubating device that a kind of temperature, dissolved oxygen are controlled |
| CN105928776A (en) * | 2016-06-13 | 2016-09-07 | 中国石油大学(北京) | Sound emission probe mounting device for true triaxial hydraulic fracturing simulation experiment |
| CN108490165A (en) * | 2018-03-21 | 2018-09-04 | 厦门大学附属第医院 | Constant temperature patch-clamp cell record system |
| CN109749929A (en) * | 2019-02-22 | 2019-05-14 | 中国人民解放军第四军医大学 | A kind of brain slice in vitro rewarming incubating device of real-time monitoring state |
| CN209727692U (en) * | 2019-03-14 | 2019-12-03 | 江西百新电瓷电气有限公司 | A kind of electric porcelain insulator, which bakes, uses temperature-detecting device |
| CN213181395U (en) * | 2020-09-07 | 2021-05-11 | 沈阳探界无损检测有限公司 | Phased array ultrasonic automatic detection device |
| CN214155990U (en) * | 2020-12-25 | 2021-09-10 | 河北医科大学 | Patch clamp perfusion drug delivery device |
-
2021
- 2021-11-17 CN CN202111364203.5A patent/CN114167042A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN204111732U (en) * | 2014-07-10 | 2015-01-21 | 中国人民解放军第四军医大学 | The diaphragm incubating device that a kind of temperature, dissolved oxygen are controlled |
| CN105928776A (en) * | 2016-06-13 | 2016-09-07 | 中国石油大学(北京) | Sound emission probe mounting device for true triaxial hydraulic fracturing simulation experiment |
| CN108490165A (en) * | 2018-03-21 | 2018-09-04 | 厦门大学附属第医院 | Constant temperature patch-clamp cell record system |
| CN109749929A (en) * | 2019-02-22 | 2019-05-14 | 中国人民解放军第四军医大学 | A kind of brain slice in vitro rewarming incubating device of real-time monitoring state |
| CN209727692U (en) * | 2019-03-14 | 2019-12-03 | 江西百新电瓷电气有限公司 | A kind of electric porcelain insulator, which bakes, uses temperature-detecting device |
| CN213181395U (en) * | 2020-09-07 | 2021-05-11 | 沈阳探界无损检测有限公司 | Phased array ultrasonic automatic detection device |
| CN214155990U (en) * | 2020-12-25 | 2021-09-10 | 河北医科大学 | Patch clamp perfusion drug delivery device |
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Application publication date: 20220311 |