CN211696518U - Device for perfusing ex vivo nerves and skeletal muscles of frogs - Google Patents
Device for perfusing ex vivo nerves and skeletal muscles of frogs Download PDFInfo
- Publication number
- CN211696518U CN211696518U CN202020847571.XU CN202020847571U CN211696518U CN 211696518 U CN211696518 U CN 211696518U CN 202020847571 U CN202020847571 U CN 202020847571U CN 211696518 U CN211696518 U CN 211696518U
- Authority
- CN
- China
- Prior art keywords
- skeletal muscle
- nerve
- liquid
- pool
- groove
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 210000002027 skeletal muscle Anatomy 0.000 title claims abstract description 96
- 210000005036 nerve Anatomy 0.000 title claims abstract description 89
- 241000269350 Anura Species 0.000 title description 3
- 239000007788 liquid Substances 0.000 claims abstract description 107
- 239000011229 interlayer Substances 0.000 claims abstract description 22
- 230000010412 perfusion Effects 0.000 claims abstract description 9
- 238000000338 in vitro Methods 0.000 claims abstract description 5
- 239000002184 metal Substances 0.000 claims abstract description 4
- 229910052751 metal Inorganic materials 0.000 claims abstract description 4
- 210000003497 sciatic nerve Anatomy 0.000 claims description 35
- 230000000638 stimulation Effects 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 210000000933 neural crest Anatomy 0.000 claims 5
- 239000010410 layer Substances 0.000 claims 2
- 230000036982 action potential Effects 0.000 abstract description 8
- 230000001537 neural effect Effects 0.000 abstract description 7
- 150000001875 compounds Chemical class 0.000 abstract description 4
- 238000000034 method Methods 0.000 abstract description 4
- 230000008569 process Effects 0.000 abstract description 4
- 238000012545 processing Methods 0.000 abstract description 4
- 238000005424 photoluminescence Methods 0.000 abstract description 2
- 230000011218 segmentation Effects 0.000 abstract description 2
- 238000012360 testing method Methods 0.000 abstract description 2
- 230000004936 stimulating effect Effects 0.000 description 11
- 238000002474 experimental method Methods 0.000 description 7
- 239000000243 solution Substances 0.000 description 6
- 239000000945 filler Substances 0.000 description 5
- 210000003205 muscle Anatomy 0.000 description 5
- 230000008827 biological function Effects 0.000 description 4
- 238000002791 soaking Methods 0.000 description 4
- 241000270722 Crocodylidae Species 0.000 description 3
- 210000001361 achilles tendon Anatomy 0.000 description 3
- 230000008602 contraction Effects 0.000 description 3
- LNNWVNGFPYWNQE-GMIGKAJZSA-N desomorphine Chemical compound C1C2=CC=C(O)C3=C2[C@]24CCN(C)[C@H]1[C@@H]2CCC[C@@H]4O3 LNNWVNGFPYWNQE-GMIGKAJZSA-N 0.000 description 3
- 210000000629 knee joint Anatomy 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 206010049816 Muscle tightness Diseases 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 210000002569 neuron Anatomy 0.000 description 2
- 230000035790 physiological processes and functions Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 241000270728 Alligator Species 0.000 description 1
- 241000269420 Bufonidae Species 0.000 description 1
- 208000008765 Sciatica Diseases 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 239000008151 electrolyte solution Substances 0.000 description 1
- 229940021013 electrolyte solution Drugs 0.000 description 1
- 230000007831 electrophysiology Effects 0.000 description 1
- 238000002001 electrophysiology Methods 0.000 description 1
- 210000003195 fascia Anatomy 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 230000004118 muscle contraction Effects 0.000 description 1
- 230000003183 myoelectrical effect Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 230000012232 skeletal muscle contraction Effects 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
- 210000000689 upper leg Anatomy 0.000 description 1
Images
Landscapes
- Measurement And Recording Of Electrical Phenomena And Electrical Characteristics Of The Living Body (AREA)
Abstract
The utility model discloses a frog in-vitro nerve and skeletal muscle perfusion device, which comprises a skeletal muscle pool, a nerve groove and a base, wherein the skeletal muscle pool is provided with a skeletal muscle pool liquid feeding pipe, a fixing clamp and five skeletal muscle pool electrodes; the nerve groove is provided with two liquid storage tanks, a back wall interlayer and a nerve groove cover plate, the liquid storage tanks are respectively provided with a nerve groove electrode, and the bottom surfaces of the tanks are respectively provided with a liquid inlet; the base is provided with fourteen wiring terminals and two liquid feeding pipe inlets and outlets; the skeletal muscle pool electrode and the nerve groove electrode are respectively connected with corresponding connecting terminals by metal leads, and the two liquid inlets are respectively connected to corresponding liquid adding pipes. The utility model discloses a have can observe neural trunk compound action potential, skeletal muscle's that electro photoluminescence induces myoelectricity and shrink tension in step, can soak respectively and handle neural trunk and skeletal muscle sample, can carry out the segmentation to neural trunk again and soak the processing, need not to remove the sample among the experimental process, and the testing data is suitable for the ration comparison, can obviously improve the stability of experimental data and the advantage of reliability.
Description
Technical Field
The utility model relates to a life science research and experiment teaching relevant device, concretely relates to frog separation nerve and skeletal muscle perfusion device.
Background
The nerve electrophysiology experiment and skeletal muscle tension measurement performed by utilizing the frog in-vitro sciatic nerve and gastrocnemius have simple requirements on experimental environment, do not need constant temperature and oxygen supply equipment in the whole experimental process, and are more applied to experimental teaching and scientific research of medicine, biology, pharmacy and the like. However, the experimental devices used at present, such as the muscle-activating device and the shielding box, have many disadvantages in practical application due to their own structural characteristics: firstly, since the isolated nerve and skeletal muscle can not be soaked by the solution, the influence of various treatment factors on the physiological functions of the nerve and skeletal muscle is not easy to observe; secondly, because the whole nerve is exposed in the same environment, the influence of treatment factors on action potential of the nerve is observed after a certain part of the nerve is not treated; thirdly, when the muscle is electrically stimulated, the needle-shaped stimulating electrode needs to be penetrated into the skeletal muscle, which is not beneficial to quantitative measurement of the skeletal muscle tension.
Disclosure of Invention
An object of the utility model is to overcome not enough among the above-mentioned existence and provide one kind and be suitable for the neural compound action potential of doing of synchronous observation electro photoluminescence induction, skeletal muscle's flesh electricity and shrink tension, can soak respectively promptly and handle neural stem and skeletal muscle sample, can carry out the segmentation to neural stem again and soak the processing, need not to remove the sample among the experimental process, and the testing data is suitable for the ration comparison to show the neural and skeletal muscle perfusion device of frog class separation that improves the stability of experimental data and reliability.
In order to achieve the above purpose, the utility model adopts the following technical scheme: the nerve cell consists of a base, a nerve groove on the base and a skeletal muscle pool on the nerve groove, wherein a base interlayer is arranged on the base, fourteen wiring terminals and two liquid inlet pipe orifices are arranged on the base interlayer, a back wall interlayer, a sciatic nerve inlet and a sciatic nerve outlet are arranged on the nerve groove, a first liquid storage pool and a second liquid storage pool are arranged in the nerve groove between the sciatic nerve inlet and the sciatic nerve outlet, a first liquid inlet is arranged at the bottom of the first liquid storage pool, a first liquid adding pipe is connected with the first liquid inlet, five nerve groove electrodes are arranged in the first liquid storage pool, a second liquid inlet is arranged at the bottom of the second liquid storage pool, a second liquid adding pipe is connected with the second liquid adding pipe, four nerve groove electrodes are arranged in the second liquid storage pool, the other ends of the first liquid adding pipe and the second liquid adding pipe are respectively arranged on the outer side of the base after passing through the back wall interlayer, the base interlayer and the corresponding liquid inlet pipe, the lower end of the skeletal muscle pool is provided with a liquid inlet, the liquid inlet is connected with a liquid feeding pipe of the skeletal muscle pool, the side wall of the skeletal muscle pool is provided with an L-shaped fixing clamp for fixing skeletal muscles, the inner wall of the skeletal muscle pool is provided with five skeletal muscle pool electrodes and nerve groove connectors, the nerve groove connectors are arranged on the sciatic nerve inlet, and the five skeletal muscle pool electrodes, the nine nerve groove electrodes and the fourteen wiring terminals are connected with metal wires arranged in the back wall interlayer and the base interlayer respectively.
Preferably, the five skeletal muscle pool electrodes are arranged on the inner walls of the skeletal muscle pool at three different heights, the highest point is arranged at a position 1.5-2.5 mm below the water level of the upper openings of the first liquid storage pool and the second liquid storage pool, the distance between adjacent different heights is set to be 20-25 mm, three electrodes are arranged near the nerve groove, the other two electrodes are arranged at two heights on the opposite side, the lowest electrode is used for connecting a ground wire, and the other electrodes are used for connecting the positive electrode and the negative electrode of the guide electrode or the stimulation electrode in an up-and-down cross manner.
Preferably, the diameter of each of the skeletal muscle pool electrode and the nerve groove electrode is 0.3-0.5 mm.
Preferably, the nerve groove electrodes are all parallel to the base and perpendicular to a connecting line between the sciatic nerve inlet and the sciatic nerve outlet, the distance between adjacent electrodes of the nerve groove electrodes is 5 mm, and the distance between the nerve groove electrode closest to the skeletal muscle pool and the inner wall of the skeletal muscle pool is 10-15 mm.
Preferably, the nerve groove and the skeletal muscle pool are designed to be transparent so as to facilitate observation.
Due to the adoption of the technical scheme, the utility model has the advantages of as follows:
1. the frog in-vitro nerve and skeletal muscle perfusion device is suitable for synchronously observing nerve trunk compound action potential induced by electric stimulation and myoelectricity and tension of skeletal muscle;
2. the frog in-vitro nerve and skeletal muscle perfusion device can be used for respectively soaking a nerve trunk and a skeletal muscle specimen and can also be used for soaking the nerve trunk in a segmented manner, so that the influence of various treatment factors on the physiological functions of the nerve trunk and the skeletal muscle can be observed;
3. because five electrodes are arranged in the skeletal muscle pool, electrolyte solutions such as ringer's solution and the like are added into the pool by utilizing the solvent conduction principle, the same experimental effect of a skeletal muscle contraction efficacy experiment obtained by directly stimulating muscles by using needle-shaped electrodes can be obtained by indirectly stimulating skeletal muscle specimens by connecting stimulation electrodes, and myoelectric changes can be simultaneously detected by connecting the leading electrodes on other skeletal muscle pool electrodes. Therefore, the experimental device is not only suitable for using skeletal muscle specimens with reserved nerves (such as sciatic nerve-gastrocnemius muscle specimens or sciatic nerve-sartorius muscle specimens) but also suitable for performing experiments by using simple skeletal muscle specimens;
4. the specimen does not need to be moved in the experimental process, so that the non-processing factors of the specimen before and after the processing factors are given can be kept unchanged, the stability and the reliability of experimental data are greatly improved, and the corresponding scientific research and experimental teaching quality is improved;
5. the electrode space in the nerve groove is short and more, the guide electrode space can be adjusted according to the length of the isolated nerve, and the nerve trunk action potential can be determined by toads or frogs with relatively small physique and short nerve.
Drawings
Fig. 1 is a schematic structural diagram of the present invention;
fig. 2 is a schematic diagram of the application of the present invention.
In the figure: 1. an L-shaped fixing clamp, 2, a skeletal muscle pool, 3, a skeletal muscle pool electrode, 4, gastrocnemius, 5, an achilles tendon ligature, 6, a skeletal muscle pool liquid feeding pipe, 7, a second liquid feeding pipe, 8, a first liquid feeding pipe, 9, a knee joint end ligature, 10, a tension transducer, 11, a double concave clamp, 12, an iron stand, 13, a nerve groove cover plate, 14, sciatic nerves, 15, a sciatic nerve spinal column ligature, 16, a nerve groove electrode, 17, a first liquid storage pool, 18, a second liquid storage pool, 19, a first liquid inlet, 20, a second liquid inlet, 21, a back wall interlayer, 22, a nerve groove, 23, a base, 24, a base interlayer, 25, a wiring terminal, 26, a nerve groove stimulating electrode, 27, a nerve groove first guiding electrode, 28, a nerve groove second guiding electrode, 29, a skeletal muscle pool stimulating electrode, 30, a skeletal muscle pool guiding electrode, 31 and a liquid inlet pipe orifice, 32. skeletal muscle pool liquid filler, 33, No. two liquid fillers, 34, No. one liquid filler, 35, sciatic nerve inlet, 36, sciatic nerve outlet, 37, nerve groove connector.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings and specific embodiments.
As shown in fig. 1, the device for perfusing ex vivo nerves and skeletal muscles provided by the utility model comprises a base 23, a nerve groove 22 on the base 23 and a skeletal muscle pool 2 on the nerve groove 22, wherein a base interlayer 24 is arranged on the base 23, fourteen connecting terminals 25 and two liquid inlet pipes 31 are arranged on the base interlayer 24, a back wall interlayer 21, an sciatic nerve inlet 35 and an sciatic nerve outlet 36 are arranged on the nerve groove 22, a first liquid storage tank 17 and a second liquid storage tank 18 are arranged in the nerve groove 22 between the sciatic nerve inlet 35 and the sciatic nerve outlet 36, a first liquid inlet 19 is arranged at the bottom of the first liquid storage tank 17, a first liquid feeding pipe 8 is connected to the first liquid inlet 19, five nerve groove electrodes 16 are arranged in the first liquid storage tank 17, a second liquid inlet 20 is arranged at the bottom of the second liquid storage tank 18, a second liquid inlet 20 is connected to a second liquid feeding pipe 7, and four nerve groove electrodes are arranged in the second liquid storage tank 18, the other ends of the first liquid adding pipe 8 and the second liquid adding pipe 7 are respectively arranged on the outer side of the base 23 after passing through the rear wall interlayer 21, the base interlayer 24 and the corresponding liquid inlet pipe mouth 31, the upper end of the nerve groove 22 is provided with a nerve groove cover plate 13, the lower end of the skeletal muscle pool 2 is provided with a liquid inlet, the liquid inlet is connected with the skeletal muscle pool liquid adding pipe 6, the side wall of the skeletal muscle pool 2 is clamped with an L-shaped fixing clamp 1 for fixing skeletal muscles, the inner wall of the skeletal muscle pool 2 is provided with five skeletal muscle pool electrodes 3 and nerve groove connectors 37, the nerve groove connectors are arranged on sciatic nerve inlets, and the five skeletal muscle pool electrodes 3, the nine nerve groove electrodes 16 and the fourteen wiring terminals 25 are respectively connected by metal wires arranged in the rear wall interlayer 21 and the base interlayer 24. The five skeletal muscle pool electrodes 3 are arranged on the inner walls of the skeletal muscle pool 2 at three different heights, the highest point is arranged at a position 1.5-2.5 mm below the water level of the upper openings of the first liquid storage pool 17 and the second liquid storage pool 18, the distance between adjacent different heights is set to be 20-25 mm, three electrodes are arranged near the nerve groove, the other two electrodes are arranged at two heights on the opposite side, the lowest electrode is used for connecting a ground wire, and the other electrodes are used for connecting the positive and negative electrodes of the guide electrode and (or) the stimulation electrode in an up-and-down cross manner. The diameter of the skeletal muscle pool electrode 3 and the diameter of the nerve groove electrode 16 are both 0.3-0.5 mm. The nerve groove electrodes 16 are all parallel to the base 23 and are perpendicular to a connecting line between the sciatic nerve inlet and the sciatic nerve outlet, the distance between adjacent electrodes of the nerve groove electrodes 16 is 5 mm, and the distance between the nerve groove electrode 16 closest to the skeletal muscle pool 2 and the inner wall of the skeletal muscle pool 2 is 10-15 mm. The nerve groove 22 and the skeletal muscle pool 2 are designed to be transparent for easy observation. The utility model discloses a theory of operation: as shown in fig. 2, a tension transducer 10 is mounted on a gantry 12 with a double-concave clamp 11.
Taking a toad or frog, destroying brain and marrow, and preparing sciatic nerve-gastrocnemius specimen without retaining femur and spine. Tying ligatures at the spinal column end of the sciatic nerve, the achilles tendon of gastrocnemius and the fascia at the end of the knee joint; taking down the L-shaped fixing clamp 1, tying the Achilles tendon ligature 5 at the lower end of the L-shaped fixing clamp 1, clamping the L-shaped fixing clamp 1 to the upper opening of the skeletal muscle pool 2, putting the gastrocnemius 4 into the skeletal muscle pool 2, and filling the musculoskeletal muscle pool liquid filler 32 with ringer's solution into the skeletal muscle pool 2 through the musculoskeletal pool liquid filler pipe 6 for soaking the gastrocnemius; tying the knee joint end ligature 9 on the cantilever arm of the tension transducer 10, adjusting the height of the fixing clamp 1 to enable the upper end of the gastrocnemius 4 not to be higher than the upper openings of the first liquid storage tank 17 and the second liquid storage tank 18, lifting the sciatic nerve spine end ligature 15, putting the sciatic nerve 14 on nerve groove electrodes 16 in the first liquid storage tank 17 and the second liquid storage tank 18, and adjusting the height of the tension transducer 10 to enable the gastrocnemius 4 to be in a natural stretching state; a first liquid adding device 34 and a second liquid adding device 33 are respectively used for filling a proper amount of wilcoxon liquid into a first liquid storage tank 17 and a second liquid storage tank 18 through a first liquid adding pipe 8 and a second liquid adding pipe 7 for soaking the sciatic nerve 14; is alongA-AThe nerve groove cover plate 13 is covered on the direction and the position is adjusted to lead the ischialgia to beIn operative contact with nerve cell electrodes 16 in reservoir No. one 17 and reservoir No. two 18 via 14.
The signal input end of the tension transducer 10 is connected to a corresponding channel of a PowerLab or BL-420 biological function experiment system and is used for recording a gastrocnemius contraction tension curve.
The crocodile clip at the front end of the nerve groove stimulating electrode 26 connected to the stimulation output of the biological function experimental system is clipped on the corresponding connecting terminal 25 connected with the nerve groove electrode 16 for electrically stimulating the sciatic nerve; the front alligator clips of the first guide electrode 27 and the second guide electrode 28 of the sciatic nerve connected to the corresponding channels of the biological function experiment system are sequentially clipped on other corresponding connecting terminals 25 connected with the nerve groove electrodes 16 for recording sciatic nerve compound action potentials.
The front end crocodile clip of the skeletal muscle pool leading electrode 30 connected to the corresponding channel of the biological function experiment system is clipped on the corresponding connecting terminal 25 connected with the skeletal muscle pool electrode 3 for recording the gastrocnemius myoelectricity; if the model is a simple gastrocnemius specimen, the crocodile clip at the front end of the skeletal muscle pool stimulating electrode 29 is clipped on the corresponding connecting terminal 25 connected with the skeletal muscle pool electrode 3, and the myoelectricity and contraction of the gastrocnemius are stimulated and induced.
When the liquid is changed, the skeletal muscle pool liquid adding device 32, the first liquid adding device 34 and the second liquid adding device 33 are used for respectively pumping the perfusion liquid in the skeletal muscle pool 2, the first liquid storage pool 17 and the second liquid storage pool 18 through the skeletal muscle pool liquid adding pipe 6, the first liquid adding pipe 8 and the second liquid adding pipe 7, and then fresh ringer's solution or other solutions serving as treatment factors are poured.
When the action potential of the sciatic nerve is measured, the liquid in the first liquid storage tank 17 and the second liquid storage tank 18 needs to be pumped out, and then the electrical stimulation is applied through the nerve groove stimulating electrode 26, so that the action potential cannot be detected due to volume conduction.
If the sciatic nerve 14 is to be electrically stimulated by the nerve channel stimulating electrode 26 to induce contraction of the gastrocnemius muscle 4, the fluid in the first reservoir 17 and the second reservoir 18 needs to be extracted, but the fluid in the skeletal muscle pool 2 does not need to be extracted; if muscle contraction is to be induced by skeletal muscle pool stimulating electrode 29, the liquid in skeletal muscle pool 2 cannot be withdrawn.
The above description is only for the preferred embodiment of the present invention, and is not intended to limit the present invention in any way, and all the modifications and equivalents of the technical spirit of the present invention to any simple modifications of the above embodiments are within the scope of the technical solution of the present invention.
Claims (5)
1. A frog in-vitro nerve and skeletal muscle perfusion device is characterized by comprising a base, a nerve groove on the base and a skeletal muscle pool on the nerve groove, wherein a base interlayer is arranged on the base, fourteen wiring terminals and two liquid inlet pipe orifices are arranged on the base interlayer, a back wall interlayer, a sciatic nerve inlet and a sciatic nerve outlet are arranged on the nerve groove, a first liquid storage pool and a second liquid storage pool are arranged in the nerve groove between the sciatic nerve inlet and the sciatic nerve outlet, a first liquid inlet is arranged at the bottom of the first liquid storage pool, a first liquid adding pipe is connected with the first liquid inlet, five nerve groove electrodes are arranged in the first liquid storage pool, a second liquid inlet is arranged at the bottom of the second liquid storage pool, a second liquid adding pipe is connected with the second liquid inlet, four nerve groove electrodes are arranged in the second liquid storage pool, the other ends of the first liquid adding pipe and the second liquid adding pipe are respectively arranged on the outer side of the base after passing through the back wall interlayer, the base interlayer and the corresponding liquid inlet, neural groove upper end is equipped with neural groove apron, and skeletal muscle pond lower extreme is equipped with into the liquid mouth, is connected with skeletal muscle pond filling tube on going into the liquid mouth, accompanies the L shape fixation clamp of a fixed skeletal muscle on the skeletal muscle pond lateral wall, is equipped with five skeletal muscle pond electrodes and neural groove connector on the skeletal muscle pond inner wall, and the neural groove connector is established on sciatic nerve entry, respectively is connected with the metal wire who establishes in back wall intermediate layer and base intermediate layer between five skeletal muscle pond electrodes and nine neural groove electrodes and fourteen binding post.
2. The device for perfusing the extracorporeal nerve and skeletal muscle of a frog in claim 1, wherein the five skeletal muscle pool electrodes are arranged on the inner walls of the skeletal muscle pool at three different heights, the highest point is arranged at the position 1.5-2.5 mm below the water level of the upper openings of the first liquid pool and the second liquid pool, the distance between the adjacent different heights is 20-25 mm, three electrodes are arranged near the nerve groove, the other two electrodes are arranged at the two heights on the opposite side, the lowest electrode is used for connecting the ground wire, and the other electrodes are used for connecting the positive electrode and the negative electrode of the guide electrode or the stimulation electrode in a vertical cross way.
3. The frog ex vivo nerve and skeletal muscle perfusion device of claim 1, wherein the diameter of each of the skeletal muscle pool electrode and the nerve groove electrode is 0.3-0.5 mm.
4. The device of claim 1, wherein the nerve groove electrodes are parallel to the base and perpendicular to the connecting line between the sciatic nerve inlet and the sciatic nerve outlet, the distance between adjacent electrodes of the nerve groove electrodes is 5 mm, and the distance between the nerve groove electrode closest to the skeletal muscle pool and the inner wall of the skeletal muscle pool is 10-15 mm.
5. The frog ex vivo nerve and skeletal muscle perfusion device of claim 1, wherein the nerve groove and skeletal muscle pool are designed to be transparent for easy observation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020847571.XU CN211696518U (en) | 2020-05-20 | 2020-05-20 | Device for perfusing ex vivo nerves and skeletal muscles of frogs |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020847571.XU CN211696518U (en) | 2020-05-20 | 2020-05-20 | Device for perfusing ex vivo nerves and skeletal muscles of frogs |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN211696518U true CN211696518U (en) | 2020-10-16 |
Family
ID=72784523
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202020847571.XU Expired - Fee Related CN211696518U (en) | 2020-05-20 | 2020-05-20 | Device for perfusing ex vivo nerves and skeletal muscles of frogs |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN211696518U (en) |
-
2020
- 2020-05-20 CN CN202020847571.XU patent/CN211696518U/en not_active Expired - Fee Related
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US6403348B1 (en) | Controlled electroporation and mass transfer across cell membranes | |
| CA2378147C (en) | Electrical impedance tomography to control electroporation | |
| McArdle et al. | Advantages of the triangularis sterni muscle of the mouse for investigations of synaptic phenomena | |
| JP2003505072A (en) | Cell / tissue analysis by controlled electroporation | |
| Graves et al. | Electrically mediated neuronal guidance with applied alternating current electric fields | |
| CN101693875A (en) | Cell electrofusion chip device based on columnar microelectrode array and electrofusion method | |
| CN211696518U (en) | Device for perfusing ex vivo nerves and skeletal muscles of frogs | |
| Nagai et al. | Patterns of conduction in smooth muscle | |
| CN106139388B (en) | Nerve electrode | |
| CN107638176A (en) | A kind of application of hydrogel in the electrode for preparing detection electrocorticogram | |
| CN111426350A (en) | Device for perfusing ex vivo nerves and skeletal muscles of frogs | |
| CN104545905B (en) | The experiment frame and experimental method of recording electrode are installed | |
| WO2010103174A1 (en) | A carbon fiber multichannel electrode for measuring electrical and chemical activity in biological tissue and a process for making the electrode | |
| CN105852855A (en) | Implantable cerebral electrode for measuring cerebral primary visual electrocorticograms in rodents | |
| US20130344559A1 (en) | Device and method for controlling nerve growth | |
| CN216998446U (en) | Cell culture dish capable of forming gradient electric field electrical stimulation | |
| US4756809A (en) | Process for conducting electrophoresis and transfer | |
| CN205411950U (en) | Combine magnetic field and supersound production vector nature not to have device of creating focus electro photoluminescence | |
| CN107789729A (en) | A kind of intrusive mood brain-computer interface | |
| Racz et al. | jULIEs: nanostructured polytrodes for low traumatic extracellular recordings and stimulation in the mammalian brain | |
| Racz et al. | jULIEs: extracellular probes for recordings and stimulation in the structurally and functionally intact mouse brain | |
| CN205586001U (en) | Be used for electrometric implanted brain electrode of muroid brain primary vision cortex brain | |
| Thoma et al. | First experimental application of multichannel stimulation devices for cardiomyoplasty | |
| CN209108405U (en) | It is a kind of for probing into the extra electric field device of potential difference around blood vessel | |
| CN109771054B (en) | Single cell marking device under in vivo electrophysiology guide |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20201016 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |