CN208200991U - The heating device of patch clamp experiments - Google Patents
The heating device of patch clamp experiments Download PDFInfo
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- CN208200991U CN208200991U CN201820385680.7U CN201820385680U CN208200991U CN 208200991 U CN208200991 U CN 208200991U CN 201820385680 U CN201820385680 U CN 201820385680U CN 208200991 U CN208200991 U CN 208200991U
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- water
- constant temperature
- thermocolumn
- cell
- temperature machine
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Abstract
The utility model discloses a kind of heating devices of patch clamp experiments, it include: a thermocolumn, one constant temperature machine and constant temperature ware, water containing chamber is equipped in the thermocolumn, helix tube snapping in perfusion tube and water containing chamber, the water inlet of thermocolumn and the water outlet of constant temperature machine connect, the water outlet of thermocolumn and the water inlet of constant temperature machine connect, the constant temperature machine has heating component and temperature control component, the constant temperature ware has a water bath, the water inlet of one end setting connection constant temperature machine of water bath, the water outlet of other end setting connection constant temperature machine, cell record slot is set in the water bath of the constant temperature ware.Thermocolumn is arranged in the utility model between perfusion tube, make the perfusate in perfusion tube in the water containing chamber for flowing through thermocolumn, the temperature requirements that the thermally conductive perfusate made in helix tube meets experiment are carried out by the warm water in the water containing chamber, guarantee the stability of cell and temperature of reagent in experimentation by the water-bath mode of circulation constant temperature water during cell experiment.
Description
Technical field
The utility model relates to the technical fields of medical instruments and equipment, particularly relate to a kind of heating dress of patch clamp experiments
It sets.
Background technique
Patch clamp technique is the research method for recording ion channel electrophysiological characteristics on full cell or respective cells film,
Research cell electrical characteristics or small-molecule drug are to ion on cell membrane when being designed to provide basic research knowledge and new drug development
The influence of channel characteristic provides a test platform for exploitation target drug.Traditional cell culture Patch Clamp System is by artificial
Operation, experimenter support research object cell in slide after obtaining primary cells (such as cardiac muscle cell and neuron)
On, record electrode movement is placed on above cell space in a manual manner and is pressed on cell membrane, recording electrode is molten outside film at this time
Resistance in liquid is about 3-9 M Ω.Cell membrane is made of bilayer lipid membrane, the characteristic with sealed insulation, therefore when record electricity
Resistance can be begun to ramp up when pole touches cell membrane, then manually to one negative pressure of application, Ke Yirang in recording electrode
Adsorb more closely between electrode and after birth and resistance can also accelerate, when the resistance of recording electrode reaches begohm
When (Giga Ω), it is meant that almost without electric current leakage between cell membrane and electrode, impose one to pressure in electrode later
Quick negative pressure, which inhales cell membrane, breaks, as soon as it will form a closed capacitor between such recording electrode and cell body, at this time
It can start to test cell.Because being leaked out around recording electrode without electric current, so that it may ensure to have recorded cell pair
The real time reaction of stimulation, such as electric current and the variation of voltage etc., and also there is very high temporal resolution and registration accuracy.It is logical
Cross the curent change for observing single channel opener and closing, can directly obtain the open current amplitude distribution of various ion channels,
The functions parameters such as open probability, open service life distribution, and the relationship between they and film potential, ion concentration etc. is analyzed, may be used also
The diaphragm of microwave tube electrode adsorption is separated from cell membrane, by the outside of film outward or film inside outward etc. in a manner of into
Row experimental study.This technology to the voltage clamping of cellule, change inside and outside film solution composition and to apply drug all very convenient.
In existing patch-clamp cell record system, the cell record slot being placed on experimental operation table including one,
Cell record slot is the development for matching film plate tongs technology and designs, and keeps patch-clamp clamp cells process smoothly complete
At.It maintains active somatic cell survival for a long time moreover, cell record slot is also to provide and controls the place that extracellular fluid swaps,
This exchange be from active somatic cell of the attached chemicals dosing plant into cell record slot or slice injection meet different requirements, through oxygen
The extracellular fluid of saturation, or injection are mixed with the extracellular fluid of the drug ingedient of different solubility, after abundant function cells, by attached
Extracellular fluid excessive in cell record slot is sucked out in the vacuum pump of category, but retains a certain amount of extracellular fluid again in track
In, it allows cell to be perhaps sliced the heavy cell record trench bottom that is attached to and is not washed away by perfusate convenient for experimenter to cell or slice
Carry out patch clamp experiments.Cell record slot, which should also become, is suitble to attached heating system to carry out heating and thermal insulation to cell record slot,
Maintain the biography temperature conductor of the psychological need of active somatic cell in cell record slot.The cell record slot of commercial type is although see at present
It is expensive like simple, but mostly imported product, in addition, existing cell record slot material is metal, easy conductive, but after being powered
Cell record slot easily experiment is interfered, influence test square wave shape and with heating device or heat chip cell
Track is expensive and easy dry combustion method.
In view of this, the designer for do not attain in the heating structure design in the above patch clamp experiments improve caused by
Many missings and inconvenience, and deeply conceive, and actively research improvement has a fling at and develops and design the utility model.
Utility model content
One kind is provided utility model aims to solve the deficiencies in the prior art will not influence experiment square-wave form,
Make the heating device of truer, the reliable patch clamp experiments of experimental data.
In order to achieve the above objectives, the solution of the utility model is:
A kind of heating device of patch clamp experiments a comprising thermocolumn, a constant temperature machine and constant temperature ware are set in the thermocolumn
There are water containing chamber, the helix tube snapping in perfusion tube and water containing chamber, the water inlet of thermocolumn and the water outlet of constant temperature machine connect, constant temperature
The water outlet of column and the water inlet of constant temperature machine connect, and the constant temperature machine has heating component and temperature control component, the constant temperature ware tool
There is a water bath, the water inlet of one end setting connection constant temperature machine of water bath, the water outlet of other end setting connection constant temperature machine should
Cell record slot is set in the water bath of constant temperature ware.
Further, a spiral coil is at least arranged in the helix tube in the water containing chamber of thermocolumn.
Further, the constant temperature machine is equipped with temperature display and temperature control button.
Further, the constant temperature machine has a water tank, and the top of water tank is set there are two the water inlet with separate valve, two into
The mouth of a river respectively by pipeline connect thermocolumn water outlet and constant temperature ware water outlet, the lower part of water tank set there are two band separate valves
The water outlet of door, two water outlets are connected the water inlet of thermocolumn and the water inlet of constant temperature ware by pipeline respectively, make the constant temperature
Recirculated water is formed in the water tank of machine.
Further, the constant temperature machine is additionally provided with anti-dry and low water level alarm device.
After adopting the above structure, compared to existing patch-clamp cell record system, the utility model patch clamp experiments
Heating device has at least the following advantages:
Firstly, the utility model makes the perfusate in perfusion tube flow through thermocolumn in thermocolumn is arranged outside perfusion tube
When water containing chamber, the temperature requirements that the thermally conductive perfusate made in helix tube meets experiment are carried out by the warm water in the water containing chamber,
And during the experiment, then by the cooperation of the water bath of constant temperature ware and cell record slot, by following during cell experiment
The water-bath mode of ring thermostatted water guarantees the stability of cell and temperature of reagent in experimentation, is not necessarily to electrified regulation, will not interfere
The form of square wave is tested, so that the data of experiment are more true, accurate, reliable.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the utility model.
Fig. 2 is the structural schematic diagram of the utility model thermocolumn.
Fig. 3 is the structural schematic diagram of the utility model constant temperature machine.
Fig. 4 is a wherein example structure schematic diagram for the utility model cell record slot and constant temperature ware.
Fig. 5 is the assembled sectional view of Fig. 4.
Fig. 6 is the exploded view of the utility model cell record slot and constant temperature ware another kind embodiment.
Fig. 7 is the constitutional diagram of embodiment illustrated in fig. 6.
Specific embodiment
In order to which the technical solution of the utility model is explained further, below by specific embodiment come to the utility model into
Row elaborates.
As shown in Figure 1, there is disclosed a kind of constant temperature patch-clamp cell record system with the utility model heating device, packet
Include a cell manipulation platform 1, a perfusion device 2, a cell record slot 3, a constant temperature ware 4, one at least a thermocolumn 5 and constant temperature machine 6.
Microwave electrodes pipe is connected on the cell manipulation platform 1, the cell record slot 3 is assemblied in the cell manipulation
On platform 1.The side of cell manipulation platform 1 is arranged in perfusion device 2, settable multiple perfusion bottles 21 on the perfusion device 2, to contain
The perfusate that different experiments require is filled, each perfusion bottle 21 can connect a perfusion tube 22, one end connection of perfusion tube 22 in bottom
Perfusion bottle 21, the other end are connected to cell record slot 3.
As shown in Fig. 2, the thermocolumn 5 is arranged between two sections of perfusion tube 22, water containing chamber is equipped in the thermocolumn 5
51, the perfusion tube 22 and 23 snapping of water containing chamber inner helix pipe, the top of the thermocolumn 5 are equipped with the water inlet of connection water containing chamber 51
52, lower part is equipped with the water outlet 53 of connection water containing chamber 51, and multiple spiral coils, spiral shell is arranged in the part that helix tube 23 is placed in water containing chamber 51
When coil 23 can make perfusate flow to the water containing chamber 51, and the warm water or the abundant heat transfer of hot water in water containing chamber 51, to make
The temperature of perfusate arrival experiment demand.
It is illustrated in figure 3 a wherein preferred embodiment for the utility model constant temperature machine 6, in the present embodiment, the constant temperature machine 6
For cylindrical body, 2/3rds can be water tank thereon, and the upper end of water tank is closed by the lid of screw lid 61, pass through the opening and closing of the screw lid 61
Pouring can be filled directly in water tank, the top of the constant temperature machine 6 sets the water inlet 62 there are two free band valve, and two water inlets 62 divide
The water outlet 53 of thermocolumn 5 and the water outlet 42 of constant temperature ware 4 are not connected by pipeline, two bands are arranged in the middle and lower part of constant temperature machine 6
The water outlet 63 of separate valve, two water outlets 63 respectively by pipeline connect thermocolumn 5 water inlet 52 and constant temperature ware 4 into
The mouth of a river 41 makes to form recirculated water in the water tank of the constant temperature machine 6.Setting adds the lower one third of the constant temperature machine 6 i.e. below water tank
The working principle of hot component and anti-dry and low water level alarm device, constant temperature machine 6 is the prior art, is not the weight of the utility model
Point, then this not be described in detail.The front of the constant temperature machine 6 is additionally provided with temperature display 64 and temperature control button 65, before experiment or
It, can be according to the temperature of specific experiment setting water in experimentation.
As shown in Figure 4, Figure 5, plate, certain cell record are made every effort to overcome for cuboid Asia in described 3 the present embodiment of cell record slot
The shape and material of slot 3 are not limited thereto, and the top surface of cell record slot 3 offers downwards a perfusion slot 31, and perfusion slot 31 is
Deep gouge, shown in as shown in the figure 1, in the present embodiment, which includes the one of two cannelures 311,312 of two cannelures 311 and connection
Translot 313 makes the perfusion slot 31 form the structure of similar " I " fonts, two cannelures 311,312 of perfusion slot 31 it is longitudinally opposed
End is respectively arcuate structure, wherein a cannelure 311 is perfusate introducing port, another cannelure 312 is perfusate export mouth, perfusate
312 longitudinal length of cannelure of export mouth is greater than the cannelure 311 of perfusate introducing port, on the cannelure 312 of the perfusate export mouth
It is provided with reference electrode hole 32.Offer a cell slot 33 in the translot 313 of the perfusion slot 31, in the present embodiment, cell slot
33 cross sectional shape is racetrack, which can also be round rectangle, which runs through the bottom surface of cell record slot 3,
The reference electrode hole 32 is consistent with the depth of cell slot 33, also extends through the bottom surface of cell record slot 3, the reference electrode hole 32
A tunnel 34 is formed between the slot bottom of cell slot 33, reference electrode hole 32 and cell slot 33 are connected to, which is L
Not on same lateral position, the bottom in tunnel 34 also extends through cell record slot 3 for type, i.e. cell slot 33 and reference electrode hole 32
Bottom surface.The bottom surface in reference electrode hole 32, cell slot 33 and tunnel 34 can be sealed by being equipped with one in the bottom surface of the cell record slot 3
Glass slide 7.
As shown in Figure 4, Figure 5, the constant temperature ware 4 has a water bath 43, and water inlet 41 is arranged in one end of water bath 43, separately
Water outlet 42 is arranged in one end, and the cell record slot 3 is arranged in water bath 43, and the bottom surface of cell record slot 3 and glass slide 7
Bottom surface and the slot bottom of water bath 43 be set in distance.The set-up mode of the cell record slot 3 and water bath 43 can there are many
Mode, for example, in water bath 43 or the bottom surface of cell record slot 3 at least one be equipped with support column, make cell record slot
There is the gap for water flowing between 3 bottom surface and the slot bottom of water bath 43.As shown, in the present embodiment, the constant temperature ware 4
For cylindrical box, the inside of the constant temperature ware 4 is water bath 43.
It as shown in Figures 4 and 5, is one of embodiment of the constant temperature ware 4 and cell record slot 3, institute in the embodiment
Stating constant temperature ware 4 is cylindrical box, and the inside of the constant temperature ware 4 is water bath 43, and the wall surface of constant temperature ware 4 is equipped with for cuboid
The groove 44 that four angles of cell record slot 3 are hooked, each angle of the cell record slot 3 rely on groove 44, make cell
The bottom of track 3 is vacantly arranged in water bath 43.The water outlet 42 of the constant temperature ware 4 is arranged below the top of cell record slot 3
End, and be higher than at the position of 3 bottom surface of cell record slot.As shown in FIG. 6 and 7, it is combined for constant temperature ware 4 with cell record slot 3
Another embodiment, in the embodiment, constant temperature ware 4 can be equally cylindrical box, and with a height of 15mm, diameter is that 90mm is
Example, the constant temperature ware 4 use translucency and the good plastic material of thermal insulation property, one water bath 43 of center setting, water bath
Be connected at the top of 43 four side wall tops and constant temperature ware, bottom vacantly with constant temperature ware bottom holding certain intervals, water bath 43 it is big
Small is 65 × 35mm, deep 5mm, and it is 10 that side wall offers multiple permeable window 45(as shown in the figure), 43 bottom of water bath is equipped with more
A permeable hole 46(is 6 as shown in the figure), in the bottom of water bath 43, at least one slot column 47, the top four of water bath 43 are set
Week can also be equipped with retaining ring 48, and the top surrounding of constant temperature ware 4 may also set up retaining ring, and cell record slot 3 is located in water bath
On 43 slot column 47, when having a certain amount of water in constant temperature ware 4, water can be passed in and out from permeable hole 46 and permeable window 45 to water bath
Recirculated water is formed in 43, and heating water bath is carried out to the cell record slot 3 in water bath 43.
When carrying out patch-clamp cell experiment, cell is placed in the cell slot 33 of cell record slot 3, cell record slot
3 are placed on cell manipulation platform 1, and constant temperature machine 6 is provided with a certain amount of water, and adjusts the constant temperature machine by temperature control button 65
The temperature of water in 6 makes water temperature meet experiment demand, and two water inlets 62 of constant temperature machine 6 are separately connected to the water outlet of constant temperature ware 4
42 and thermocolumn 5 water outlet 53, two water outlets 63 of constant temperature machine 6 are separately connected the water inlet 41 and thermocolumn 5 of constant temperature ware 4
Water inlet 62, during the experiment, by manipulate valve make the water in the constant temperature machine 6 formed circulation warm water, while thermocolumn 5 and
Recirculated water is similarly formed in constant temperature ware 4.The switch for opening perfusion tube 22 makes the perfusate in perfusion bottle 21 flow into perfusion tube 22
In, when perfusate flows through thermocolumn 5, the warm water in thermocolumn 5 can heat the perfusate in helix tube 23, special helix tube
23 form spiral coil in thermocolumn 5, make the abundant heat transfer of perfusate in the warm water and helix tube 23 in the thermocolumn 5, make
Perfusate temperature in helix tube 23 meets experiment demand.Perfusate after heating from the cannelure 311 of cell record slot 3 be perfusion
Liquid introducing port flows into, and flows into the cell slot 33 in perfusion slot 31 after the cell wall of cannelure 311 buffering, the water inlet of water bath 43
41 connections connect constant temperature machine 6 by pipeline, continue into warm water, the water outlet 42 of water bath 43 is persistently discharged, makes in water bath 43
Water form recirculated water, cell record slot 3 is carried out to continue heat or tepidarium, preferably, for preferable heat insulation effect, constant temperature
Ware 4, thermocolumn 5, connection thermocolumn 5 and constant temperature machine 6, connect constant temperature machine 6 and each pipeline of constant temperature ware 4 and can adopt perfusion tube 22
With the good plastic material production of thermal insulation property, perfusion helix tube then uses the good plastic material production of heating conduction.
The product form and style of above-described embodiment and schema and non-limiting the utility model, any technical field
The appropriate changes or modifications that those of ordinary skill does it all should be regarded as the patent category for not departing from the utility model.
Claims (5)
1. a kind of heating device of patch clamp experiments characterized by comprising a thermocolumn, a constant temperature machine and constant temperature ware, the perseverance
Water containing chamber, the helix tube snapping in perfusion tube and water containing chamber, the water inlet of thermocolumn and the water outlet of constant temperature machine are equipped in Wen Zhu
Connection, the water outlet of thermocolumn and the water inlet of constant temperature machine connect, and the constant temperature machine has heating component and temperature control component, described
Constant temperature ware has a water bath, the water inlet of one end setting connection constant temperature machine of water bath, and other end setting connects constant temperature machine
Water outlet, the water bath of the constant temperature ware is interior to be arranged cell record slot.
2. the heating device of patch clamp experiments as described in claim 1, it is characterised in that: in the water containing chamber of thermocolumn
A spiral coil is at least arranged in helix tube.
3. the heating device of patch clamp experiments as described in claim 1, it is characterised in that: the constant temperature machine is aobvious equipped with temperature
Display screen and temperature control button.
4. the heating device of patch clamp experiments as described in claim 1, it is characterised in that: the constant temperature machine has a water tank,
The top of water tank is set there are two the water inlet with separate valve, two water inlets respectively by pipeline connect thermocolumn water outlet and
The water outlet of constant temperature ware, the lower part of water tank are set there are two the water outlet with separate valve, and two water outlets are connected by pipeline respectively
The water inlet of thermocolumn and the water inlet of constant temperature ware are connect, makes to form recirculated water in the water tank of the constant temperature machine.
5. the heating device of the patch clamp experiments as described in any one of Claims 1-4, it is characterised in that: the constant temperature
Machine is additionally provided with anti-dry and low water level alarm device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820385680.7U CN208200991U (en) | 2018-03-21 | 2018-03-21 | The heating device of patch clamp experiments |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820385680.7U CN208200991U (en) | 2018-03-21 | 2018-03-21 | The heating device of patch clamp experiments |
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| Publication Number | Publication Date |
|---|---|
| CN208200991U true CN208200991U (en) | 2018-12-07 |
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| CN201820385680.7U Expired - Fee Related CN208200991U (en) | 2018-03-21 | 2018-03-21 | The heating device of patch clamp experiments |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115369021A (en) * | 2022-08-15 | 2022-11-22 | 安徽省立医院(中国科学技术大学附属第一医院) | Multipurpose flow cytometry washing instrument, control method and application |
-
2018
- 2018-03-21 CN CN201820385680.7U patent/CN208200991U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115369021A (en) * | 2022-08-15 | 2022-11-22 | 安徽省立医院(中国科学技术大学附属第一医院) | Multipurpose flow cytometry washing instrument, control method and application |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20181207 Termination date: 20210321 |