CN108490165A - Constant temperature patch-clamp cell record system - Google Patents
Constant temperature patch-clamp cell record system Download PDFInfo
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- CN108490165A CN108490165A CN201810235057.8A CN201810235057A CN108490165A CN 108490165 A CN108490165 A CN 108490165A CN 201810235057 A CN201810235057 A CN 201810235057A CN 108490165 A CN108490165 A CN 108490165A
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- constant temperature
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/1031—Investigating individual particles by measuring electrical or magnetic effects thereof, e.g. conductivity or capacity
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
Abstract
The invention discloses a kind of constant temperature patch-clamp cell record systems, it includes a cell manipulation platform, one be arranged on cell manipulation platform cell record slot, one be connected to the perfusion device of cell record slot, a constant temperature ware coordinated with cell record slot, the thermocolumn that is arranged on perfusion device and one connect thermocolumn and constant temperature ware constant temperature machine；Thermocolumn is arranged outside in perfusion tube in the present invention, make the perfusate in perfusion tube in the water containing chamber for flowing through thermocolumn, heat conduction is carried out by the warm water in the water containing chamber by perfusate in perfusion tube is made to meet the temperature requirements tested, and during the experiment, then pass through the cooperation of the water bath and cell record slot of constant temperature ware, ensure the stability of cell and temperature of reagent in experimentation by the water-bath mode of circulation constant temperature water during cell experiment, without electrified regulation, will not interference experiment square wave form so that the data of experiment are more true, accurate, reliable.
Description
Technical field
The present invention relates to the technical fields of medical instruments and equipment, particularly relate to a kind of constant temperature patch-clamp cell record system.
Background technology
Patch clamp technique is the research method for recording ion channel electrophysiological characteristics on full cell or respective cells film,
Basic research knowledge is designed to provide with research cell electrical characteristics or small-molecule drug when new drug development to ion on cell membrane
The influence of channel characteristic provides a test platform for exploitation target drug.Traditional cell culture Patch Clamp System is by artificial
Operation, experimenter support research object cell in slide after obtaining primary cells (such as cardiac muscle cell and neuron)
On, recording electrode movement is placed on above cell space in a manual manner and is pressed onto on cell membrane, recording electrode is molten outside film at this time
Resistance in liquid is about 3-9 M Ω.Cell membrane is made of bilayer lipid membrane, the characteristic with sealed insulation, therefore when record electricity
Resistance can be begun to ramp up when pole touches cell membrane, then manually to applying a negative pressure, Ke Yirang in recording electrode
Adsorb more closely between electrode and after birth and resistance can also accelerate, when the resistance of recording electrode reaches begohm
When (Giga Ω), it is meant that leaked out almost without electric current between cell membrane and electrode, impose one to pressure in electrode later
Quick negative pressure, which inhales cell membrane, breaks, as soon as a closed capacitance can be formed between such recording electrode and cell body, at this time
It can start to test cell.Because being leaked out around recording electrode without electric current, so that it may to ensure to have recorded cell pair
The real time reaction of stimulation, for example, electric current and voltage variation etc., and also have very high temporal resolution and registration accuracy.It is logical
Cross the curent change for observing single channel opener and closing, can directly obtain current amplitude distribution that various ion channels open,
The functions parameters such as open probability, open service life distribution, and the relationship between they and film potential, ion concentration etc. is analyzed, may be used also
The diaphragm of microwave tube electrode adsorption is separated from cell membrane, by the outside of film outward or film inside outward etc. in a manner of into
Row experimental study.This technology to the voltage clamping of cellule, change inside and outside film solution composition and to apply drug all very convenient.
In patch-clamp cell record system, including a cell record slot being placed on experimental operation table, cell note
Recording slot is designed for the development of matching film plate tongs technology, and patch-clamp clamp cells process is enable to smoothly complete.Moreover,
Cell record slot, which is also to provide, to be maintained active somatic cell survival for a long time and controls the place that extracellular fluid swaps, this exchange
Be active somatic cell from from attached chemicals dosing plant to cell record slot or slice injection meet different requirements, through oxygen-saturated thin
Extracellular fluid, or injection are mixed with the extracellular fluid of the drug ingedient of different solubility, after abundant function cells, by attached vacuum
Excessive extracellular fluid in cell record slot is sucked out in pump, but retains a certain amount of extracellular fluid again in track, allows cell
Either the heavy cell record trench bottom that is attached to of slice is not washed away convenient for experimenter by perfusate to cell or slice progress patch-clamp
Experiment.Cell record slot, which should also become, is suitble to attached heating system to carry out heating and thermal insulation to cell record slot, maintains cell note
Record the biography temperature conductor of the psychological need of active somatic cell in slot.The cell record slot of commercial type is although seem simple at present,
Mostly imported product, it is expensive, in addition, there is also following disadvantages for existing cell record slot：1, material be metal, easy conductive,
Cell record slot after energization easily interferes experiment, influences the shape for testing square wave；2, the perfusion of existing cell record slot
Mouth is low, same plane is almost in cell liquid, perfusate is easy to wash away cell when importing the cell record slot；3, go out fluid level
It is low, when aspirating perfusate, it is not easy to hold the balance of disengaging perfusate, is easily drained extracellular fluid, cell loss；4, reference electricity
Pole placement mouth is too big, when moving glass carrier bench, easily reference electrode is made to shake, influences the square-wave form of experiment；5, band heating device or
Heat chip cell record slot is expensive and easy dry combustion method.
In view of this, the designer for above-mentioned patch-clamp cell record system design on do not attain improve caused by it is many
Missing and inconvenience, and deeply conceive, and actively research improvement has a fling at and develops and design the present invention.
Invention content
There is provided a kind of at low cost it is an object of the invention to solve the deficiencies in the prior art, experimental data is more true,
Reliable constant temperature patch-clamp cell record system.
In order to achieve the above objectives, solution of the invention is：
A kind of constant temperature patch-clamp cell record system comprising a cell manipulation platform, a perfusion device, a cell record slot, one
Constant temperature ware, a constant temperature machine and an at least thermocolumn；The cell record slot is arranged on cell manipulation platform, on perfusion device at least
The perfusion bottle for containing perfusate equipped with one, perfusion bottle connect a perfusion tube, and one end of perfusion tube connects perfusion bottle, other end connection
Cell record slot, the thermocolumn are arranged between the both ends of perfusion tube, and water containing chamber is equipped in the thermocolumn, and the helix tube is set
In in the water containing chamber, the water inlet of thermocolumn and the water outlet of constant temperature machine connect, the water inlet of the water outlet and constant temperature machine of thermocolumn
Mouth connection, the constant temperature machine have heating component and temperature control component, and the top surface of the cell record slot offers downwards a perfusion
Slot, perfusion slot are deep gouge, and wherein one end of the perfusion slot is perfusate introducing port, and the perfusion tube is connected to perfusate importing
Mouthful, the other end is perfusate export mouth, and perfusion slot offers a cell slot between both ends, and perfusion slot is additionally provided with one and cell slot
The consistent reference electrode hole of depth, reference electrode hole are connected to by a tunnel with the slot bottom of cell slot, the bottom surface of cell slot, reference
The bottom surface of electrode hole and the bottom surface in tunnel combine a glass slide, the constant temperature ware that there is a water bath, one end of water bath to be arranged
The water inlet of constant temperature machine, the water outlet of other end setting connection constant temperature machine are connected, the cell record slot is arranged in water bath,
And the bottom surface of cell record slot and the slot bottom of the bottom surface of glass slide and water bath are set in distance.
Further, a spiral coil is at least arranged in the helix tube in the water containing chamber of thermocolumn.
Further, the constant temperature machine is equipped with temperature display and temperature control button.
Further, the constant temperature machine has a water tank, and the top of water tank is set there are two the water inlet with separate valve, two into
The mouth of a river connects the water outlet of thermocolumn and the water outlet of constant temperature ware by pipeline respectively, and the lower part of water tank sets that there are two band separate valves
The water outlet of door, two water outlets are connected the water inlet of thermocolumn and the water inlet of constant temperature ware by pipeline, make the constant temperature respectively
Recirculated water is formed in the water tank of machine.
Further, the constant temperature machine is additionally provided with anti-dry and low water level alarm device.
Further, the perfusion slot includes two pods and the translot for being connected to two pods, and the perfusion slot is made to form " work " word
Type structure, the cell slot are opened in the translot of perfusion slot, wherein a pod is perfusate introducing port, another pod is perfusion
Liquid export mouth.
Further, perfusate introducing port is arcuate structure, and the pod of perfusate export mouth is more than the vertical of perfusate introducing port
Slot, the reference electrode hole are arranged in the pod of perfusate export mouth.
Further, the cross sectional shape of the cell slot is round rectangle or endless track.
Further, the bottom surface of the cell slot, tunnel and reference electrode hole perforation cell record slot, the glass slide bonding
In the bottom surface of cell record slot, and seal cell slot, tunnel and reference electrode hole.
Further, the tunnel is L-type.
Further, the constant temperature ware is cylindrical box, and the inside of the constant temperature ware is water bath, on the wall surface of water bath and
Bottom is equipped with permeable window and permeable hole for constant temperature water flowing, and water-bath trench bottom vacantly keeps a determining deviation with constant temperature ware bottom.
Further, the water bath is equipped with support column, makes the bottom surface of cell record slot vacantly between the slot bottom of water bath
In the presence of the gap for water flowing.
Further, it is surrounded by retaining ring at the top of water bath and constant temperature ware, prevents water excessive.
Further, the water outlet of the water bath is arranged below groove body top, and is higher than at the position of groove body bottom surface.
With the above structure, compared to existing patch-clamp cell record system, constant temperature patch-clamp cell note of the present invention
Recording system has at least the following advantages：
First, the present invention makes the perfusate in perfusion tube in the water containing chamber for flowing through thermocolumn in thermocolumn is arranged outside perfusion tube,
Heat conduction is carried out by the warm water in the water containing chamber by perfusate is made to meet the temperature requirements tested, and during the experiment, then lead to
The cooperation for crossing the water bath and cell record slot of constant temperature ware is protected during cell experiment by the water-bath mode of circulation constant temperature water
The stability of cell and temperature of reagent during confirmation is tested is not necessarily to electrified regulation, will not interference experiment square wave form so that it is real
The data tested are more true, accurate, reliable.
Secondly, cell slot of the invention is provided in perfusion slot, and perfusion slot is made not in the same plane, to be filled in cell slot
When stream, perfusate can flowed into first after being buffered in perfusion slot in cell slot, make the cell in cell slot be not easy to be flushed away, together
Sample is easy to hold the balance of liquid in-out due to going out horizontal plane height, when suction, extracellular fluid is not easy to be taken out when perfusate is flowed out
Dry, cell will not be lost substantially, the accuracy and reliability of same certifiable experimental data.
Furthermore reference electrode of the invention places mouth and is of moderate size, diameter about 4mm, ensure reference electricity when mobile glass carrier bench
The stability of pole does not influence the square-wave form of experiment so that the data of experiment are more true, accurate, reliable.
Compared to the cell record slot with heating device or heating chip, acrylic can be used in cell record slot of the invention
Material, material facilitate buying and making, and at low cost.
Description of the drawings
Fig. 1 is the structural diagram of the present invention.
Fig. 2 is the structural schematic diagram of thermocolumn of the present invention.
Fig. 3 is the structural schematic diagram of constant temperature machine of the present invention.
Fig. 4 is the exploded view of cell record slot of the present invention and constant temperature ware embodiment.
Fig. 5 is the constitutional diagram of embodiment illustrated in fig. 4.
Specific implementation mode
In order to further explain the technical solution of the present invention, being explained in detail the present invention below by specific embodiment
It states.
As shown in Figures 1 to 5, present invention is disclosed a kind of constant temperature patch-clamp cell record systems comprising a cell is grasped
Make platform 1, a perfusion device 2, a cell record slot 3, a constant temperature ware 4, one at least a thermocolumn 5 and constant temperature machine 6.
Microwave electrodes pipe 11 is connected on the cell manipulation platform 1, the cell record slot 3 is assemblied in cell behaviour
Make on platform 1.Perfusion device 2 is arranged in the side of cell manipulation platform 1, and multiple perfusion bottles 21 can be arranged on the perfusion device 2, to
The perfusate that different experiments require is contained, each perfusion bottle 21 can connect a perfusion tube 22 in bottom, and perfusion bottle 21 passes through perfusion
Pipe connects with thermocolumn upper end, and thermocolumn lower end connects with one end of another perfusion tube, and the other end then connects cell record slot 3.
As shown in Fig. 2, the thermocolumn 5 is arranged between the both ends of perfusion tube 22, water containing chamber is equipped in the thermocolumn 5
51, the helix tube 23 is placed in the water containing chamber 51, and the top of the thermocolumn 5 is equipped with the water inlet 52 of connection water containing chamber 51, under
Portion is equipped with the water outlet 53 of connection water containing chamber 51, and helix tube 23 is placed in water containing chamber 51 and multiple spiral coils are arranged, and helix tube 23 can make
When perfusate flow to the water containing chamber 51, and the warm water or the abundant heat transfer of hot water in water containing chamber 51, to make perfusate reach
The temperature of experiment demand.
It is illustrated in figure 3 a wherein preferred embodiment for constant temperature machine 6 of the present invention, in the present embodiment, the constant temperature machine 6 is circle
Cylinder, 2/3rds can be water tank thereon, and the upper end of water tank is covered by screw lid 61 closes, can be straight by the keying of the screw lid 61
It is connected to fill in water tank and pour, the top of the constant temperature machine 6 sets the water inlet 62 there are two free band valve, and two water inlets 62 lead to respectively
Piping connects the water outlet 53 of thermocolumn 5 and the water outlet 42 of constant temperature ware 4, and two bands are arranged independently in the middle and lower part of constant temperature machine 6
The water outlet 63 of valve, two water outlets 63 connect the water inlet 52 of thermocolumn 5 and the water inlet of constant temperature ware 4 by pipeline respectively
41, make to form recirculated water in the water tank of the constant temperature machine 6.Heating group is arranged in the lower one third of the constant temperature machine 6 below water tank
The operation principle of part and anti-dry and low water level alarm device, constant temperature machine 6 is the prior art, is not the emphasis of the present invention, then this
Not it is described in detail.The front of the constant temperature machine 6 is additionally provided with temperature display 64 and temperature control button 65, tests preceding or experimentation
In, the temperature of water can be set according to specific experiment.
As shown in Figure 4, Figure 5, plate, certain cell record are made every effort to overcome for cuboid Asia in described 3 the present embodiment of cell record slot
The shape and material of slot 3 are not limited thereto, and the top surface of cell record slot 3 offers downwards a perfusion slot 31, and perfusion slot 31 is
Deep gouge, shown in as shown in the figure 1, in the present embodiment, which includes the one of two pods 311,312 of two pods 311 and connection
Translot 313 makes the perfusion slot 31 form the structure of similar " I " fonts, two pods 311,312 of perfusion slot 31 it is longitudinally opposed
End is respectively arcuate structure, wherein a pod 311 is perfusate introducing port, another pod 312 is perfusate export mouth, perfusate
312 longitudinal length of pod of export mouth is more than the pod 311 of perfusate introducing port, on the pod 312 of the perfusate export mouth
It is provided with reference electrode hole 32.Offer a cell slot 33 in the translot 313 of the perfusion slot 31, in the present embodiment, cell slot
33 cross sectional shape is racetrack, which is alternatively round rectangle, which runs through the bottom surface of cell record slot 3,
The reference electrode hole 32 is consistent with the depth of cell slot 33, also extends through the bottom surface of cell record slot 3, the reference electrode hole 32
A tunnel 34 is formed between the slot bottom of cell slot 33, reference electrode hole 32 and cell slot 33 are connected to, which is L
With reference electrode hole 32 not on same lateral position, the bottom in tunnel 34 also extends through cell record slot 3 for type, i.e. cell slot 33
Bottom surface.The bottom surface in reference electrode hole 32, cell slot 33 and tunnel 34 can be sealed equipped with one in the bottom surface of the cell record slot 3
Glass slide 7.
There is the constant temperature ware 4 water bath 43, one end of constant temperature ware 4 water inlet 41 is arranged, and water outlet is arranged in the other end
42, the cell record slot 3 is arranged in water bath 43, and the bottom surface and water bath of the bottom surface of cell record slot 3 and glass slide 7
43 slot bottom is set in distance.As shown in Figures 4 and 5, the embodiment combined with cell record slot 3 for constant temperature ware 4, the implementation
In example, constant temperature ware 4 can be equally cylindrical box, and with a height of 15mm, for a diameter of 90mm, which uses translucency
And the good plastic material of heat-insulating property, one water bath 43 of center setting, four side wall tops and the constant temperature ware of water bath 43
Top is connected, and bottom vacantly keeps certain intervals with constant temperature ware bottom, and the size of water bath 43 is 65 × 35mm, deep 5mm, side wall
Offer multiple permeable windows 45（As shown in the figure is 10）, 43 bottom of water bath is equipped with multiple permeable holes 46（As shown in the figure is 6
It is a）, at least one slot column 47 is set in the bottom of water bath 43, the top surrounding of water bath 43 can also be equipped with retaining ring 48, permanent
The top surrounding of warm ware 4 may also set up retaining ring, and cell record slot 3 is located on the slot column 47 of water bath 43, when constant temperature ware 4
When inside having a certain amount of water, water can form recirculated water out of permeable hole 46 and the disengaging to water bath 43 of permeable window 45, to water-bath
Cell record slot 3 in slot 43 carries out heating water bath.
When carrying out patch-clamp cell experiment, cell is placed in the cell slot 33 of cell record slot 3, cell record slot
3 are placed on cell manipulation platform 1, a certain amount of water are housed, and adjust the constant temperature machine by temperature control button 65 in constant temperature machine 6
The temperature of water in 6 makes water temperature meet experiment demand, and two water inlets 62 of constant temperature machine 6 are separately connected to the water outlet of constant temperature ware 4
42 and thermocolumn 5 water outlet 53, two water outlets 63 of constant temperature machine 6 are separately connected the water inlet 41 and thermocolumn 5 of constant temperature ware 4
Water inlet 62, during the experiment, by manipulate valve make the water in the constant temperature machine 6 formed cycle warm water, while thermocolumn 5 and
Recirculated water is similarly formed in constant temperature ware 4.The switch for opening perfusion tube 22 makes the perfusate in perfusion bottle 21 flow into perfusion tube 22
In, when perfusate flows through thermocolumn 5, the warm water in thermocolumn 5 can heat the perfusate in helix tube 23, special helix tube
23 form spiral coil in thermocolumn 5, make the warm water in the thermocolumn 5 and the abundant heat transfer of perfusate, make in perfusion tube 22
Perfusate temperature meets experiment demand.Perfusate after heating from the pod 311 of cell record slot 3 be perfusate introducing port stream
Enter, flowed into the cell slot 33 in perfusion slot 31 after the cell wall of pod 311 buffering, the connection of water inlet 41 of water bath 43 passes through
Pipeline connects constant temperature machine 6, continues into warm water, the water outlet 42 of water bath 43 is persistently discharged, and so that the water in water bath 43 is formed and is followed
Ring water carries out cell record slot 3 to continue heat or tepidarium, preferably, for preferable heat insulation effect, constant temperature ware 4, perfusion tube
22, thermocolumn 5, connection thermocolumn 5 and constant temperature machine 6, connect constant temperature machine 6 and heat-insulating property can be used in each pipeline of constant temperature ware 4
Good plastic material makes.5 inner helix pipe 23 of thermocolumn is adopted as good heat-conducting.
The product form and style of above-described embodiment and schema and the non-limiting present invention, any technical field it is common
The appropriate changes or modifications that technical staff does it all should be regarded as the patent category for not departing from the present invention.
Claims (10)
1. a kind of constant temperature patch-clamp cell record system comprising a cell manipulation platform, a perfusion device, a cell record slot,
One constant temperature ware, a constant temperature machine and an at least thermocolumn；The cell record slot is arranged on cell manipulation platform, perfusion device up to
Be equipped with a perfusion bottle for containing perfusate less, perfusion bottle connected by perfusion tube with thermocolumn upper end, thermocolumn lower end with it is another
One end of perfusion tube connects, and the other end then connects cell record slot, and the thermocolumn is arranged between the both ends of perfusion tube, the perseverance
Water containing chamber is equipped in Wen Zhu, the perfusion tube is connected by snap fit with the helix tube of thermocolumn, the water inlet of thermocolumn and
The water outlet of constant temperature machine connects, and the water outlet of thermocolumn and the water inlet of constant temperature machine connect, and the constant temperature machine has heating component
And temperature control component, the top surface of the cell record slot offer downwards a perfusion slot, perfusion slot is deep gouge, and the perfusion slot is wherein
One end is perfusate introducing port, and the perfusion tube is connected to the perfusate introducing port, and the other end is perfusate export mouth, perfusion slot
A cell slot is offered between both ends, perfusion slot is additionally provided with a reference electrode hole consistent with cell groove depth, reference electrode
Hole is connected to by a tunnel with the slot bottom of cell slot, and the bottom surface of the bottom surface of cell slot, the bottom surface in reference electrode hole and tunnel combines
One glass slide, the constant temperature ware have a water bath, the water inlet of one end setting connection constant temperature machine of water bath, other end setting
The water outlet of constant temperature machine is connected, the cell record slot is arranged in water bath, and the bottom surface of cell record slot and glass slide
The slot bottom of bottom surface and water bath is set in distance.
2. constant temperature patch-clamp cell record system as described in claim 1, it is characterised in that：In the water containing chamber of thermocolumn
Helix tube one spiral coil is at least set.
3. constant temperature patch-clamp cell record system as described in claim 1, it is characterised in that：The constant temperature machine is equipped with temperature
Display screen and temperature control button.
4. constant temperature patch-clamp cell record system as described in claim 1, it is characterised in that：The constant temperature machine has a water
The top of case, water tank is set there are two the water inlet with separate valve, and two water inlets connect the water outlet of thermocolumn by pipeline respectively
The lower part of the water outlet of mouth and constant temperature ware, water tank is set there are two the water outlet with separate valve, and two water outlets pass through pipe respectively
Road connects the water inlet of thermocolumn and the water inlet of constant temperature ware, makes to form recirculated water in the water tank of the constant temperature machine.
5. the constant temperature patch-clamp cell record system as described in any one of Claims 1-4, it is characterised in that：The perseverance
Warm machine is additionally provided with anti-dry and low water level alarm device.
6. constant temperature patch-clamp cell record system as described in claim 1, it is characterised in that：The perfusion slot includes two pods
And a translot of two pods of connection, so that the perfusion slot is formed dies, the cell slot is opened in the translot of perfusion slot
Interior, wherein a pod is perfusate introducing port, another pod is perfusate export mouth.
7. constant temperature patch-clamp cell record system as claimed in claim 6, it is characterised in that：Perfusate introducing port is arc knot
Structure, the pod of perfusate export mouth are more than the pod of perfusate introducing port, and the reference electrode hole is arranged in perfusate export mouth
Pod in.
8. constant temperature patch-clamp cell record system as described in claim 1, it is characterised in that：The cross sectional shape of the cell slot
For round rectangle or endless track.
9. constant temperature patch-clamp cell record system as described in claim 1, it is characterised in that：The cell slot, tunnel and ginseng
Than the bottom surface that electrode hole penetrates through cell record slot, the glass slide is bonded in the bottom surface of cell record slot, and seals cell slot, tunnel
Road and reference electrode hole.
10. the constant temperature patch-clamp cell record system as described in claim 1 or 9, it is characterised in that：The tunnel is L-type.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810235057.8A CN108490165A (en) | 2018-03-21 | 2018-03-21 | Constant temperature patch-clamp cell record system |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810235057.8A CN108490165A (en) | 2018-03-21 | 2018-03-21 | Constant temperature patch-clamp cell record system |
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| CN108490165A true CN108490165A (en) | 2018-09-04 |
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| CN201810235057.8A Withdrawn CN108490165A (en) | 2018-03-21 | 2018-03-21 | Constant temperature patch-clamp cell record system |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109374875A (en) * | 2018-11-20 | 2019-02-22 | 中国科学院生物物理研究所 | Low noise constant temperature gravity perfusion patch clamp |
| CN110987767A (en) * | 2019-11-11 | 2020-04-10 | 中国科学院地质与地球物理研究所 | Air pressure micromanipulation system and method for micron-sized particle sample |
| CN111321052A (en) * | 2018-12-14 | 2020-06-23 | 中国科学院深圳先进技术研究院 | Cell recording groove device and cell recording method |
| CN111659482A (en) * | 2020-06-11 | 2020-09-15 | 南昌航空大学 | Device for quickly and accurately controlling temperature of quartz microbalance flow cell and construction method thereof |
| CN113406316A (en) * | 2021-06-17 | 2021-09-17 | 重庆医科大学附属儿童医院 | Electrophysiological patch clamp perfusion device |
| CN114167042A (en) * | 2021-11-17 | 2022-03-11 | 上海交通大学 | Multifunctional platform for patch clamp recording and use method thereof |
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| CN109374875A (en) * | 2018-11-20 | 2019-02-22 | 中国科学院生物物理研究所 | Low noise constant temperature gravity perfusion patch clamp |
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| CN110987767A (en) * | 2019-11-11 | 2020-04-10 | 中国科学院地质与地球物理研究所 | Air pressure micromanipulation system and method for micron-sized particle sample |
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| CN113406316A (en) * | 2021-06-17 | 2021-09-17 | 重庆医科大学附属儿童医院 | Electrophysiological patch clamp perfusion device |
| CN113406316B (en) * | 2021-06-17 | 2023-08-08 | 重庆医科大学附属儿童医院 | Electrophysiology patch clamp perfusion device |
| CN114167042A (en) * | 2021-11-17 | 2022-03-11 | 上海交通大学 | Multifunctional platform for patch clamp recording and use method thereof |
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