CN206580827U - Gene sequencing electrophoresis tank - Google Patents
Gene sequencing electrophoresis tank Download PDFInfo
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- CN206580827U CN206580827U CN201621406521.8U CN201621406521U CN206580827U CN 206580827 U CN206580827 U CN 206580827U CN 201621406521 U CN201621406521 U CN 201621406521U CN 206580827 U CN206580827 U CN 206580827U
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- electrophoresis tank
- water
- glass plate
- gene sequencing
- groove
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Abstract
The utility model discloses a kind of gene sequencing electrophoresis tank, including water jacket, the inside groove that can be positioned in water jacket and the groove lid that can be placed on above water jacket, groove lid is provided with the anode interface being connected with supply unit and negative pole interface, it is provided with above inside groove with groove lid just, positive electricity pole and negative electricity pole that negative pole interface matches, inside groove also includes glue plate detachably with installation, outer groove bottom can lay and fix the rectangle pit of glue plate provided with one, during glue, only need to be by insertion pit after spill glass plate and flat glass plate laminating, both sides can coordinate clamp, it is compared to traditional electrophoresis tank, special gum-making rack or gel maker is not needed, it is convenient and simple；It is additionally provided with circulating cooling system, Intelligent adjustment temperature, cooling-down effect substantially and stably, reduces damage of the heat to electrophoresis tank instrument, and then increase service life.
Description
Technical field
The utility model is related to gene molecule biological experiment instrument, and in particular to a kind of nucleic acid electrophoresis tank technical field,
More particularly to a kind of gene sequencing electrophoresis tank for integrating glue and electrophoresis.
Background technology
The tropism of electrically charged material in the electric field is referred to as electrophoresis.Nucleic acid electrophoresis is the important hand for carrying out nucleic acids research
Section, is the indispensable part of the technologies such as nucleic acid probe, nucleic acid amplification and sequence analysis.Nucleic acid electrophoresis is generally in agar
Carried out in sugared gel or polyacrylamide gel, the different agarose of concentration and polyacrylamide can form molecule sieve screen apertures size
Different gels, the nucleic acid fragment available for separation different molecular weight.
Electrophoresis tank is the core of gel electrophoresis system, and the fast development of its system is also mainly to be embodied in electrophoresis tank
On.According to the principle of electrophoresis, gel is all placed between two cushion chambers, and electric field connects two cushion chambers by gel, for example
Vertical slab electrophoresis groove and level board electrophoresis tank etc..
Nucleic acid electrophoresis mainly includes glue, the polymerization of glue sample, loading, electrophoresis, detection and evaluation etc., and existing electrophoresis tank is
After sample makes, the progress electrophoresis process that is wherein powered is put into, and glue needs to prepare by special gum-making rack or gel maker, than
It is more complicated, trouble, and also the glue sample prepared is at room temperature, and polymerization time is long, and efficiency is low.In addition, during electrophoresis, medium can be caused to send out
Heat, so need to cool to it, and existing cooling is usual by the way of ice cube or water cooling, existing these cooling methods cooling
Effect is unstable or undesirable, influences the accuracy of testing result.
To overcome disadvantage mentioned above, the design people is subject to positive innovation research, to create a kind of new gene sequencing
Use electrophoresis tank.
Utility model content
The utility model is mainly solving the technical problems that provide a kind of gene sequencing electrophoresis tank, collection glue and electrophoresis mistake
Journey is in one, it is not necessary to special gum-making rack or gel maker, convenient and simple, while shorten the glue time, and also it is cold provided with circulating
But system, substantially, stability is high for cooling-down effect, and security is good.
In order to solve the above technical problems, the technical scheme that the utility model is used is：A kind of gene sequencing is provided to use
Electrophoresis tank, including water jacket, the inside groove that can be positioned in water jacket and the groove lid that can be placed on above water jacket, the groove lid arranged on left and right sides
Anode interface and negative pole interface are respectively equipped with, the anode interface and the negative pole interface are all electrically connected with supply unit；It is described
Arranged on left and right sides is respectively equipped with the positive electricity pole and negative electricity pole matched with groove lid positive and negative electrode interface, the inside groove above inside groove
Also include glue plate detachably with installation, the inner bottom surface of the water jacket can lay and fix the rectangle of the glue plate provided with one
Pit；
Provided with that can lead to the spacer of cooling water on the outside of the water jacket, the spacer is provided with water inlet and delivery port, the water inlet
Mouth is provided with water intaking valve, and the delivery port is provided with outlet valve, and the water inlet connects water circulating pump by the water intaking valve, described to follow
Ring water pump connects external tank, and temperature sensor is provided between the spacer and the water jacket；
Including control unit, the temperature sensor, the water intaking valve, the outlet valve and the water circulating pump all with control
Cell signal connection processed, described control unit is electrically connected with the supply unit.
In order to solve the above technical problems, the further technical scheme that the utility model is used is：
The glue plate includes a spill glass plate and the flat glass plate matched with the spill glass plate.
Further say, the concave surface of the spill glass plate forms a rectangular enclosure after being fitted with the flat glass plate.
Further say, the concave glass plate or the flat glass plate top are directed at mouth provided with pipette tips.
Further say, the positive electricity pole and the negative electricity pole are all platinum electrode.
Further say, the positive electricity pole extends to inside groove exterior bottom by wire.
Further say, the width of the rectangular enclosure is 1.5mm.
Further say, the electrophoresis tank is vertical slab electrophoresis groove.
The beneficial effects of the utility model are：The utility model at least has advantages below：
First, the utility model outer slot bottom is provided with pit, during glue, and only spill glass plate and flat glass plate need to be fitted
Afterwards in insertion pit, both sides can coordinate clamp, be compared to traditional electrophoresis tank, it is not necessary to special gum-making rack or
Gel maker, it is convenient and simple；
2nd, the utility model is provided with circulating cooling system and temperature sensor, and electrophoresis process can release substantial amounts of heat, temperature
Sensor can it is real-time to water temperature monitor and be transmitted to control unit, control unit by controlling water circulating pump, water intaking valve and outlet valve,
Cooling water is set to be circulated in spacer, Intelligent adjustment temperature, cooling-down effect substantially and stably, reduces the damage to electrophoresis tank instrument,
And then increase service life；
3rd, due to provided with circulating cooling system, after glue, when colloid polymerize, due to heat can be released, opening cooling water and following
Loop system, compared to that at room temperature, can greatly shorten the gel polymerisation time, improves efficiency；
4th, glue plate top of the present utility model is directed at mouth provided with pipette tips, during glue, is easy to liquid-transfering gun to pinpoint liquid feeding, prevents
Only the pipette tips movement of liquid-transfering gun, produces bubble, influences experiment effect；
5th, the utility model is provided with groove lid, glue and electrophoresis process safety.
Described above is only the general introduction of technical solutions of the utility model, in order to better understand skill of the present utility model
Art means, and being practiced according to the content of specification, with preferred embodiment of the present utility model and coordinate accompanying drawing detailed below
Describe in detail bright as after.
Brief description of the drawings
Fig. 1 is the utility model overall structure diagram；
Fig. 2 is the overall overlooking the structure diagram of the utility model；
Fig. 3 is the overlooking the structure diagram of water jacket of the present utility model；
Each several part mark is as follows in accompanying drawing：
Water jacket 1, pit 11, spacer 12, water inlet 121, delivery port 122, inside groove 2, positive electricity pole 21, negative electricity pole 22,
Glue plate 23, spill glass plate 231, flat glass plate 232, rectangular enclosure 233, groove lid 3, anode interface 31, negative pole interface 32
With temperature sensor 4.
Embodiment
Illustrate embodiment of the present utility model below by way of particular specific embodiment, those skilled in the art can
Advantage of the present utility model and effect are understood by content disclosed in the present specification easily.The utility model can also it is other not
Same mode is practiced, i.e. under the scope of without departing substantially from disclosed in the utility model, can give different modifications and change.
Embodiment：A kind of gene sequencing electrophoresis tank, as shown in Figure 1, Figure 2 and Figure 3, including water jacket 1, can be positioned over water jacket
Inside groove 2 in 1 is respectively equipped with anode interface 31 and negative pole with that can be placed on the groove lid 3 of the top of water jacket 1, the arranged on left and right sides of groove lid 3
Interface 32, the anode interface 31 and the negative pole interface 32 are all electrically connected with supply unit；The top of inside groove 2 left and right two
Side is respectively equipped with the positive electricity pole 21 and negative electricity pole 22 matched with groove lid positive and negative electrode interface, and the inside groove 2 also includes removable
The glue plate 23 with installing is unloaded, the inner bottom surface of the water jacket 1 can lay and fix the rectangle pit of the glue plate 23 provided with one
11；
The outside of water jacket 1 is provided with water inlet 121 and delivery port provided with that can lead to the spacer 12 of cooling water, the spacer 12
122, the water inlet 121 is provided with water intaking valve, and the delivery port 122 is provided with outlet valve, and the water inlet passes through the water intaking valve
Water circulating pump is connected, the water circulating pump connects external tank, and temperature sensor is provided between the spacer 12 and the water jacket 1
4；
Including control unit, the temperature sensor 4, the water intaking valve, the outlet valve and the water circulating pump all with
Control unit signal is connected, and described control unit is electrically connected with the supply unit.
The glue plate 23 includes a spill glass plate 231 and the flat glass matched with the spill glass plate 231
Plate 232.
The concave surface of the spill glass plate 232 forms a rectangular enclosure 233 after being fitted with the flat glass plate 231.
Concave glass plate 231 or the flat glass plate 232 top is directed at mouth provided with pipette tips.
The positive electricity pole 21 and the negative electricity pole 22 are all platinum electrode.
The positive electricity pole 22 extends to the exterior bottom of inside groove 2 by wire.
From top view direction, the width of the rectangular enclosure 233 is 1.5mm.
The electrophoresis tank is vertical slab electrophoresis groove.
Operation principle of the present utility model is as follows：
Article and instrument needed for preparing experiment, by insertion outer slot bottom after spill glass plate and flat glass plate laminating
In pit, both sides can be fixed with clamp, and the pipette tips being then aligned with liquid-transfering gun on glue plate are directed at mouth liquid feeding, and liquid feeding is completed
Afterwards, groove lid is covered, cooling recirculation system is opened, solidifies gel polymerisation, then take out glue plate, be installed in inside groove, then
Sequentially add buffer solution, sample-adding etc., cover groove lid afterwards, the positive and negative electrode interface of groove lid will in inside groove positive and negative electrode post it is relative
Should, switch on power, you can carry out electrophoresis process, in the presence of stationary electric field, nucleic acid can displacement, different fragments or molecule
The nucleic acid translational speed of amount is different, after electrophoresis terminates, and coordinates Other Instruments to carry out coherent detection and evaluation；
Substantial amounts of heat can be released in electrophoresis process, temperature sensor real-time to water temperature can monitor and be transmitted to control unit, controls
Unit processed is by controlling water circulating pump, water intaking valve and outlet valve, Intelligent adjustment temperature, and cooling-down effect substantially and stably, is reduced pair
The injury of electrophoresis tank instrument, and then increase service life.
Embodiment of the present utility model is the foregoing is only, the scope of the claims of the present utility model is not thereby limited, it is every
The equivalent structure made using the utility model specification and accompanying drawing content, or directly or indirectly it is used in other related technologies
Field, is similarly included in scope of patent protection of the present utility model.
Claims (8)
1. a kind of gene sequencing electrophoresis tank, including water jacket, the inside groove that can be positioned in water jacket and the groove that can be placed on above water jacket
Lid, it is characterised in that：The groove lid arranged on left and right sides is respectively equipped with anode interface and negative pole interface, the anode interface and described
Negative pole interface is all electrically connected with supply unit；Arranged on left and right sides is respectively equipped with and groove lid positive and negative electrode interface phase above the inside groove
The positive electricity pole and negative electricity pole matched somebody with somebody, the inside groove also include glue plate detachably with installation, and the inner bottom surface of the water jacket is set
There is one can lay and fix the rectangle pit of the glue plate；
Provided with that can lead to the spacer of cooling water on the outside of the water jacket, the spacer is provided with water inlet and delivery port, and the water inlet is set
There is water intaking valve, the delivery port is provided with outlet valve, and the water inlet connects water circulating pump, the recirculated water by the water intaking valve
Pump connects external tank, and temperature sensor is provided between the spacer and the water jacket；
Including control unit, the temperature sensor, the water intaking valve, the outlet valve and the water circulating pump are all single with control
First signal connection, described control unit is electrically connected with the supply unit.
2. gene sequencing electrophoresis tank according to claim 1, it is characterised in that：The glue plate includes a spill glass
Plate and the flat glass plate matched with the spill glass plate.
3. gene sequencing electrophoresis tank according to claim 2, it is characterised in that：The concave surface of the spill glass plate and institute
A rectangular enclosure is formed after stating flat glass plate laminating.
4. gene sequencing electrophoresis tank according to claim 2, it is characterised in that：The concave glass plate or the plane
Glass plate top is directed at mouth provided with pipette tips.
5. gene sequencing electrophoresis tank according to claim 1, it is characterised in that：The positive electricity pole and the negative electrode
Post is all platinum electrode.
6. gene sequencing electrophoresis tank according to claim 1, it is characterised in that：The positive electricity pole is extended by wire
To inside groove exterior bottom.
7. gene sequencing electrophoresis tank according to claim 1, it is characterised in that：The width of the rectangular enclosure is
1.5mm。
8. gene sequencing electrophoresis tank according to claim 1, it is characterised in that：The electrophoresis tank is vertical slab electrophoresis
Groove.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621406521.8U CN206580827U (en) | 2016-12-21 | 2016-12-21 | Gene sequencing electrophoresis tank |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621406521.8U CN206580827U (en) | 2016-12-21 | 2016-12-21 | Gene sequencing electrophoresis tank |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206580827U true CN206580827U (en) | 2017-10-24 |
Family
ID=60108018
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621406521.8U Active CN206580827U (en) | 2016-12-21 | 2016-12-21 | Gene sequencing electrophoresis tank |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206580827U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108342314A (en) * | 2018-04-02 | 2018-07-31 | 通用生物系统（安徽）有限公司 | Condense vertical oligonucleotides electrophoretic apparatus |
| CN109971752A (en) * | 2019-04-04 | 2019-07-05 | 通用生物系统（安徽）有限公司 | A kind of method of extensive primer condensation Purified in electrophoresis separation |
-
2016
- 2016-12-21 CN CN201621406521.8U patent/CN206580827U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108342314A (en) * | 2018-04-02 | 2018-07-31 | 通用生物系统（安徽）有限公司 | Condense vertical oligonucleotides electrophoretic apparatus |
| CN109971752A (en) * | 2019-04-04 | 2019-07-05 | 通用生物系统（安徽）有限公司 | A kind of method of extensive primer condensation Purified in electrophoresis separation |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20190618 Address after: 102600 Beijing Daxing District Beijing Economic and Technological Development Zone No. 156 Courtyard Building 401 Jinghai Fourth Road Patentee after: Beijing Qingke Biotechnology Co.,Ltd. Address before: 102209 No. 64 Litang Road, Beiqijiazhen, Changping District, Beijing Patentee before: BEIJING TSINGKE XINYE BIOLOGICAL TECHNOLOGY CO.,LTD. |
|
| TR01 | Transfer of patent right | ||
| CP03 | Change of name, title or address |
Address after: 100176 Floor 6, West Half Unit, Building 3, Yard 105, Jinghai 3rd Road, Daxing District, Beijing Economic-Technological Development Area Patentee after: Beijing Qingke Biotechnology Co.,Ltd. Address before: 401, building 5, No. 156, Jinghai 4th Road, Beijing Economic and Technological Development Zone, Daxing District, Beijing 102600 Patentee before: Beijing Qingke Biotechnology Co.,Ltd. |
|
| CP03 | Change of name, title or address |