CN201727504U - Synchronous tester for isolated heart physiological characteristics - Google Patents
Synchronous tester for isolated heart physiological characteristics Download PDFInfo
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- CN201727504U CN201727504U CN 200920103299 CN200920103299U CN201727504U CN 201727504 U CN201727504 U CN 201727504U CN 200920103299 CN200920103299 CN 200920103299 CN 200920103299 U CN200920103299 U CN 200920103299U CN 201727504 U CN201727504 U CN 201727504U
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Abstract
The utility model provides a synchronous tester for isolated heart physiological characteristics, which relates to the technical field of biological experiment devices. The synchronous tester is provided with a constant-temperature and constant-pressure bath, a specimen bath and an oxygen saturation K-H liquid perfusion device; the constant-temperature and constant-pressure bath is provided with an annular tubular constant-temperature liquid container; the specimen bath is mounted into a cylindrical space of the annular tubular constant-temperature liquid container; the oxygen saturation K-H liquid perfusion device is provided with an oxygen saturation K-H liquid perfusion pressure container and a guide pipe passing through the annular tubular constant-temperature liquid container; and the synchronous tester is provided with two or three of the following testing devices: a, a coronary flow metering device; b, an electrocardiogram testing device; and c, a myocardial contractility testing device. The synchronous tester can test different performance indexes in constant-temperature and constant-pressure state and in the same time, can eliminate influences on the performance indexes in different time, can reveal change of comprehensive performance indexes of different physical and chemical factors in the same time, can further completely and objectively reveal the functions of the physical and chemical factors on a heart, and is accurate in test and is highly scientific.
Description
Technical field
This utility model relates to the biology experimental installation technical field.
Background technology
The physiology characteristic of isolated heart comprises shrinkage characteristics, electrology characteristic and the coronary flow of cardiac muscle.At present, in student experimenting and some scientific researches, mostly the test of these indexs is to carry out respectively.According to the animal difference of using, method that is adopted and device are also inequality.
1. amphibian animal (Bufo siccus) isolated heart:
1) observation of isolated heart function: adopt eight-Mu Shi method or Si Shi method more,, add medicine in the liquid, to observe it to the contract influence of power of the heart with RingerShi (appoint Ge Shi) liquid perfusion heart.Eight-Mu Shi method is that tremulous pulse and vein all insert conduit, and the organizator outer circulation changes the chemical factors in the perfusate, observes stroke volume and kinemic variation.The Si Shi method is that stenonian duct is inserted tremulous pulse, adds the variation of observed drug myocardial contraction.These methods are more ancient outmoded, coarse, cut to be difficult for the record cardiac output, can not use microcomputer, so this method can not be used for scientific research, only are used for student experimenting, and the physiological property of heart is done qualitative observation.
2) isolated frog heart meter drip apparatus: isolated frog heart stroke volume and kinemic measurement, the isolated frog heart apparatus for counting drops (ZL 02253158.0) that adopts us to develop more.This device uses the isolated heart of Bufo siccus, because animal is low etc., also only is applicable to student experimenting, observes myocardial contraction, the cardiac rhythm phenomenon that contracts of relaxing.This method energy instrumentation stroke volume and cardiac output can not surveyed coronary flow, also are difficult for the thought-read electrograph.Can not be used for mammal.
2. mammals isolated heart: often adopt the Langendorff method, insert arterial cannulation at the isolated heart tremulous pulse, with oxygen-saturated Krebs-Henseleit solution (K-H liquid) or lockerSolution reverse perfusions such as (locke solutions).Insert small water balloon in the ventricle, the systolic pressure water squeezing capsule of ventricle shows at microcomputer by pressure transducer; Perhaps observe the contract variation of power of the heart by tension transducer.But this method is difficult for record ECG; Use graduated cylinder to measure coronary flow, the range estimation metering easily produces error; And making material is glass.It is fragile..
3. heart surface ECG: available once exhausted improvement Langendorff method or frog electrocardio volume conductor bootstrap technique.ECG with the volume conductor principle record frog heart or mammals isolated heart.But these methods can not be remembered the thought-read output, also the inconvenience note thought-read power that contracts.
Above-mentioned test someway, although also obtained the data of some physical signs, some problems are described, as be not at one time in instrumentation, thereby test result is not comprehensive, reference value is little, because each test data can not reflect the variation of the cardiac function in the same time, can not get rid of the influence that different time produces each performance indications, error is big, and is scientific poor.
Summary of the invention
The purpose of this utility model provides a kind of isolated heart physiology characteristic synchrometer, can and carry out the test of different performance index under the constant temperature and pressure state at one time, can get rid of the influence that different time produces each performance indications, the integrated performance index that can reflect the same time, and test accurately, can objectively respond the influence of chemical factors to cardiac function comprehensively, scientific strong.
Main technical schemes of the present utility model is: a kind of isolated heart physiology characteristic synchrometer, it is characterized in that: have temperature chamber, specimen bath and nutritional solution constant voltage perfusion device, the specimen bath is loaded in the temperature chamber, nutritional solution constant voltage perfusion device is provided with nutritional solution pressure vessel and guide wire thereof, guide wire passes through from temperature chamber, the top that the outlet of guide wire is positioned at the specimen bath connects arterial cannulation, arterial cannulation can insert in the aorta of isolated heart, and the specimen bath is provided with the discharge flow-guiding structure; And have in the following test set two kinds or 3 kinds, and all link to each other with microcomputer, show coronary flow, electrocardiogram or myocardial contraction:
A, coronary flow metering device;
B, electrocardiogram test set;
C, myocardial contraction force test device.
In the described test set two kinds or 3 kinds all pass through BL-420E
+Be connected to goodly with microcomputer, or link to each other with microcomputer by other forms of transducer.
Described nutritional solution is oxygen-saturated K-H liquid or locke solution etc.
The preferred construction of described temperature chamber is: have cylinder shape isothermal liquid container, cylinder shape isothermal liquid container is provided with liquid-inlet and liquid outlet, is provided with the constant temperature pump between liquid-inlet and liquid outlet.Described temperature chamber also can be cylinder shape constant temperature gas container, or the bottom of cylinder shape isothermal liquid container and part top also can establish the liquid constant temperature interlayer, etc. structure.
Described guide wire multi-turn is wrapped on the interior annular wall of cylinder shape isothermal liquid container for good, to guarantee that the preheating perfusate is a constant temperature.Generally should make temperature stabilization at the 36-37 degree.
Electrode in the described electrocardiogram test set inserts mechanism for can 360 ° of rotatable cover formulas being good, has covered rotating handle; On the temperature chamber cage azimuth scale can be arranged, the convenient use.
The exit portion of described guide wire is fixed on the rotary support for good, with handled easily.
Good effect of the present utility model is: the deficiency that has overcome prior art, can be under the constant temperature and pressure state, and carry out at one time can not performance indications test, can get rid of the influence that different time produces each performance indications, can reflect integrated performance index in the same cardiac cycle, and test accurately can objectively respond chemical factors to action of the heart and influence comprehensively, and is scientific strong.
But use this device synchronous recording isolated heart coronary flow, electrocardiogram and three important physical signs of myocardial contraction heart, also can write down out wherein two or one certainly easily; Use microcomputer to show, remember and survey and the statistical experiment result, be convenient to fast the result be analyzed and adds up, make experiment have more accuracy, science; The device volume I is carried out in screened room in addition, strengthens anti-jamming capacity, makes ECG clear attractive in appearance especially; Make the inexpensive economy of material, be applicable to medical science or biology student's physiology, pathology and the scientific research of pharmacological evaluation and relevant heart, the development and the exploitation of heart physiological and treatment cardiotropic formulation had important Practical significance.
Be described further below in conjunction with embodiment and accompanying drawing, but not as to qualification of the present utility model.
Description of drawings
Fig. 1 is a structural representation of the present utility model.
Among Fig. 1, each label implication is: 1. temperature chamber, 101. liquid-inlets, 102. liquid outlets, 103. cage (indicating angle), 104. cylinder shape isothermal liquid container, 2. guide wire, 201 guide wire imports, 202. guide wire outlet, 203. arterial cannulation (connecing heart), 3. specimen bath, 4. (electrocardiogram) electrode insertion mechanism, (401. electrode inserts mechanism) rotating handle, 402. electrode 5. rotates and the lift support 501. rotation and lifting framves, 502. fixed bar, 6. (myocardial contraction force test device) small water balloon, 7. (oxygen-saturated K-H liquid perfusion) pressure vessel (as infusion bottle), 8.BL-420E
+(or claim BL-420E
+The biological function system), 9. air deflector, 10. funneling minim pipette, 11. bipolar electrode pins, 12. spout holes, 13. waste liquid tanks, 14. memory electrode formula liquid droplet counting devices, 15. housings.
The specific embodiment
See Fig. 1, this isolated heart physiology characteristic synchrometer, have temperature chamber 1, specimen bath 3 and nutritional solution constant voltage perfusion device, specimen bath 3 is loaded in the temperature chamber 1, nutritional solution constant voltage perfusion device is provided with nutritional solution pressure vessel 7 (being infusion bottle etc.) and guide wire 2 thereof, guide wire 2 passes through from temperature chamber 1,201 are the guide wire inlet, 202 are the guide wire outlet, be positioned at the top of specimen bath 3, guide wire outlet 202 connects arterial cannulation 203, and arterial cannulation 203 and isolated heart are communicated with, and specimen bath 3 is provided with the discharge flow-guiding structure; And have following test set, and all link to each other with microcomputer, show coronary flow, electrocardiogram or myocardial contraction: a, coronary flow metering device; B, electrocardiogram test set; C, myocardial contraction force test device; All pass through BL-420E
+(not shown) links to each other with microcomputer (can same prior art).Nutritional solution is oxygen-saturated K-H liquid or locke solution.The structure of temperature chamber 1 is: have cylinder shape isothermal liquid container 104, cylinder shape isothermal liquid container 104 is provided with liquid-inlet 101 and liquid outlet 102, and 102 of liquid-inlet 101 and liquid outlets are provided with constant temperature pump (not shown).Guide wire 2 multi-turns are wrapped in (for the hosepipe shape) on the interior annular wall of cylinder shape isothermal liquid container 1.The structure of coronary flow metering device is: for meter drips formula, the discharge flow-guiding structure that specimen bath 3 is provided with is an air deflector 9, the below of air deflector 9 is provided with funneling minim pipette 10, and the below of funneling minim pipette 10 is provided with waste liquid tank 13, and the below of funneling minim pipette 10 is provided with the liquid droplet counting device.The liquid droplet counting device is memory electrode formula liquid droplet counting device or photo-electric liquid droplet counting device.It is 360 ° of rotatable cover formulas that electrode in the electrocardiogram test set inserts mechanism, has covered rotating handle 401.Guide wire 2 exit portion are fixed on rotation and the lift support 5.The myocardial contraction force test device can same prior art.
The mammal isolated heart only under constant temperature and pressure and oxygen-saturated nutritional solution perfusion condition, just has irritability, otherwise specimen can very fast death, the failure of an experiment.So the Langendorff method is to put a Serpentis type glass tubing in a glass tubing again, flowing in the hosepipe is liquid such as K-H liquid or Le Shi, and pipe is outer to be constant temperature liquid.Our device is to make a circular rings tubular isothermal liquid container, and linking number circle plastic tube in container guarantees that perfusate is homothermic.
The specimen bath: this bath is put in the temperature chamber, is beneficial to the perfusate insulation.Oxygen-saturated nutritional solution enters the temperature chamber preheating by import, and outlet links to each other constant temperature liquid perfusion heart with arterial cannulation.Liquid flow approach: K-H liquid → guiding tube → heart → specimen bath → side opening → liquid guide needle (capillary siphoning principle) → funnel → note dropping electrode or photoelectricity drop recorder.
Pin type note dropping electrode: the past meter drips uses tricks to drip rod more, often writes down failure because of the liquid adhesion, adopts thin note to drip pin, and with its curved down 35 degree, has just avoided shortcoming in the past, and success rate improves.Langendorff instrumentation coronary flow can only use graduated cylinder to collect, the range estimation metering.Because animal hearts is little, the hat flow is little, and range estimation easily produces error.Use our method instead, make it convert drop to the signal of telecommunication, convert square wave to through the drop transducer, microcomputer shows instrumentation.
Electrode insertion mechanism is 360 degree rotary types in the described electrocardiogram test set.Electrode is put into the specimen bath.According to research purpose, rotating electrode with the definite optimum position that can show ECG of optimal seeking method, utilizes this electrode can write down the heart surface ECG of any point, isolated heart surface.As use this test set research heart ischemia to irritate the myocardial damage that causes again, and detect electricity (just) utmost point and aim at ventricle, can be observed the evolution process of T ripple in ischemia and the filling process again, can record the change procedure of myocardial contraction curve and coronary flow simultaneously.
The heart stick is fixed on note drips on the box, thermostatic bath and specimen bath are placed on above the funnel.The preparation isolated heart is inserted small water balloon in it, the electrocardiographic recorder electrode is put into the specimen bath, and the move operation bar is also put into the specimen bath with isolated heart, connects BL-420E
+, connect with microcomputer again, can experimentize.
Manipulator: but the manipulator that is provided with left-right rotation and moves up and down, and stick links to each other with arterial cannulation.So just can arbitrarily change the locus of isolated heart, in order to better operation, as in ventricle, inserting small water balloon.Langendorff method and other methods do not have this device.
Electrocardiogram, myocardial contraction and coronary flow are very important three physical signs of reaction cardiac function.Any chemical factors all can reflect in these three indexs the influence of cardiac function.In the past, in the experiment of physiology, pharmacology and pathology or in the scientific research.React certain chemical factors to action of the heart with above-mentioned one or two index that records someway.Although the result also can illustrate some problems with these method gained, and is not too comprehensive.Because the meaning difference that each index shows, the variation of coronary flow is except that outside the Pass having with cardiac muscle easypro contract (resistance), and is also relevant with smooth muscle arteria coronaria pipe (caliber size); Electrocardiogram is the generation and the conduction of reaction heart excitement; What myocardial contraction directly reflected is the functional status of myocardial cell.
These physical signs all have necessary relation with cardiac cycle.If three's synchronous recording is come out, just can reflect comprehensively that chemical factors is to action of the heart.In order to observe of the influence of certain factor at organ level to cardiac electric variation, metataxis and arteria coronaria smooth muscle, rule according to the volume conductor conduction, with reference to all multi-methods of past isolated heart perfusion, this coronary flow, ECG and myocardial contraction synchronous recording device have been developed.To experimentize better and scientific research.
Preparation animal (as rat) isolated heart inserts arterial cannula, will be through temperature chamber preheating, oxygen-saturated k-h liquid or the reverse perfusion of tyrode constant voltage.Perfusate underwent coronary nutrition heart makes it keep certain irritability.Flow into the liquid of specimen bath by arteria coronaria, flow to funnel through its sidewall aperture, the drop that drips is again through meter dropping electrode and drop transducer, is presented on the microcomputer with the form of square wave.According to the rule of volume conductor conduction, by recording electrode in the bath, the heart surface ecg of record isolated heart.Rotatable because of electrode, heart can move up and down again, thus can clearly record the electrocardiogram at any position of heart, as atrium repolarization wave (Ta ripple).Insert small water balloon in the left ventricle, the easypro extruding water pocket that contracts of ventricle through pressure transducer, shows at microcomputer, writes down out the ventricular systole curve.This device can be observed the influence of chemical factors to heart at organ level.This equipment manufacturing cost is cheap, can be used for physiology, pathology and pharmacological evaluation and the correlational study thereof of medical science or students of agricultural college, for about treatment and prevention of cardiac drug development and exploitation important practical value being arranged.
As use guinea pig isolated heart, its coronary flow of test record simultaneously, myocardial contraction and heart surface ecg (summary).
Claims (9)
1. isolated heart physiology characteristic synchrometer, it is characterized in that: have temperature chamber (1), specimen bath (3) and nutritional solution constant voltage perfusion device, specimen bath (3) is loaded in the temperature chamber (1), nutritional solution constant voltage perfusion device is provided with nutritional solution pressure vessel (7) and guide wire (2) thereof, guide wire (2) passes through from temperature chamber (1), the outlet of guide wire (202) is positioned at the top of specimen bath (3) and connects arterial cannulation (203), and specimen bath (3) is provided with the discharge flow-guiding structure; And have in the following test set two kinds or 3 kinds, and all link to each other with microcomputer, show coronary flow, electrocardiogram or myocardial contraction:
A, coronary flow metering device;
B, electrocardiogram test set;
C, myocardial contraction force test device.
2. isolated heart physiology characteristic synchrometer according to claim 1 is characterized in that two kinds or 3 kinds in the described test set, all passes through BL-420E
+Link to each other with microcomputer.
3. isolated heart physiology characteristic synchrometer according to claim 1 is characterized in that described nutritional solution is oxygen-saturated K-H liquid or locke solution.
4. isolated heart physiology characteristic synchrometer according to claim 1, the structure that it is characterized in that described temperature chamber (1) is: have cylinder shape isothermal liquid container (104), cylinder shape isothermal liquid container (104) is provided with liquid and advances (101) and liquid outlet (102), and liquid advances between (101) and liquid outlet (102) to be provided with the constant temperature pump.
5. isolated heart physiology characteristic synchrometer according to claim 4 is characterized in that described guide wire (2) multi-turn is wrapped on the interior annular wall of cylinder shape isothermal liquid container (1).
6. isolated heart physiology characteristic synchrometer according to claim 1, the structure that it is characterized in that described coronary flow metering device is: for meter drips formula, the discharge flow-guiding structure that specimen bath (3) is provided with is air deflector (9), the below of air deflector (9) is provided with funneling minim pipette (10), the below of funneling minim pipette (10) is provided with waste liquid tank (13), and the below of funneling minim pipette (10) is provided with the liquid droplet counting device.
7. isolated heart physiology characteristic synchrometer according to claim 6 is characterized in that described liquid droplet counting device is memory electrode formula liquid droplet counting device or photo-electric liquid droplet counting device.
8. isolated heart physiology characteristic synchrometer according to claim 1 is characterized in that the electrode insertion mechanism in the described electrocardiogram test set is 360 ° of rotatable cover formulas, has covered rotating handle (401).
9. according to claim 1,2,3,4,5,6,7 or 8 described isolated heart physiology characteristic synchrometers, it is characterized in that described guide wire (2) exit portion is fixed on rotation and the lift support (5).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200920103299 CN201727504U (en) | 2009-06-17 | 2009-06-17 | Synchronous tester for isolated heart physiological characteristics |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200920103299 CN201727504U (en) | 2009-06-17 | 2009-06-17 | Synchronous tester for isolated heart physiological characteristics |
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| CN201727504U true CN201727504U (en) | 2011-02-02 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102274078A (en) * | 2011-05-13 | 2011-12-14 | 沈阳医学院 | Experimental device for changing toad heart afterload |
| CN104897886A (en) * | 2015-05-27 | 2015-09-09 | 广东省中医院 | Constant-temperature incubator for isolated perfused heart |
| CN106215317A (en) * | 2016-07-18 | 2016-12-14 | 安徽师范大学 | Stimulating electrode device |
| CN112707017A (en) * | 2021-01-07 | 2021-04-27 | 首都医科大学 | Insulation can for separation heart experiments |
-
2009
- 2009-06-17 CN CN 200920103299 patent/CN201727504U/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102274078A (en) * | 2011-05-13 | 2011-12-14 | 沈阳医学院 | Experimental device for changing toad heart afterload |
| CN104897886A (en) * | 2015-05-27 | 2015-09-09 | 广东省中医院 | Constant-temperature incubator for isolated perfused heart |
| CN104897886B (en) * | 2015-05-27 | 2017-02-22 | 广东省中医院 | Constant-temperature incubator for isolated perfused heart |
| CN106215317A (en) * | 2016-07-18 | 2016-12-14 | 安徽师范大学 | Stimulating electrode device |
| CN112707017A (en) * | 2021-01-07 | 2021-04-27 | 首都医科大学 | Insulation can for separation heart experiments |
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| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110202 Termination date: 20110617 |