CN207923885U - Amphibian Perfused isolated heart device - Google Patents
Amphibian Perfused isolated heart device Download PDFInfo
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- CN207923885U CN207923885U CN201820357075.9U CN201820357075U CN207923885U CN 207923885 U CN207923885 U CN 207923885U CN 201820357075 U CN201820357075 U CN 201820357075U CN 207923885 U CN207923885 U CN 207923885U
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- electrocardio
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Abstract
The utility model is related to a kind of amphibian Perfused isolated heart devices, it includes arterial cannulation, venous cannula, note dropping electrode and electrocardio leading electrode, the arterial cannulation connects arterial cannulation trunk, aorta insertion end and drain pipe by a triple valve and is composed, the venous cannula connects liquid storage tank, posterior vena cava insertion end and liquid-feeding tube by a triple valve and is composed, and each component grafting of arterial cannulation and venous cannula is combined into one.The note dropping electrode includes that note dropping electrode head, note dropping electrode bar and signal input part, the electrocardio leading electrode include electrocardio leading electrode head, electrocardio leading electrode bar, signal input part, heart outer wall liquid-feeding tube and triple valve.The multi-functional amphibian Perfused isolated heart device is suitable for one man operation, is conveniently replaceable perfusate, is suitable for the dynamic change of a variety of parameters of left ventricular function such as synchronous dynamic detection cardiac output, myocardial contraction tension and electrocardio.
Description
Technical field
The utility model is related to a kind of life science related scientific research and experimental teaching experimental provisions, and in particular to one kind two
It dwells animal Perfused isolated heart device.
Background technology
Can intuitively observe the activity of heart using Perfused isolated heart system, study heart systolic and diastolic function, heart rate and
The influence of rhythm and pace of moving things variation and various humoral factors to cardiac function, is one of the important means of research cardiac function, answers extensively
For teaching and scientific researches such as medicine, biology, pharmacy.The tradition carried out using the amphibians isolated heart such as toad or batrachia is double
Tube method（Yagi spark gap-Hartung methods）Cardiac perfusion device can intuitively observe whole heart contraction, have and be not required to constant temperature and oxygen supply
The advantages that equipment, cardiomotility is not adjusted by neurohumor.The flowing of perfusate one-way circulation meets heart working in double-tube method
Physiological characteristic is suitable for studying variation and influence of the various processing factors to cardiac systolic function of front and back load.But it is traditional
For double-tube method due to the design feature of perfusion device itself, perfusion preparation of specimen is more complex, and one man operation is difficult；Suction pipe liquid feeding need to be used
Or drawing liquid, rinse or change liquid inconvenience；It is sequential to apply different perfusates（Processing factor）When, easily touch device with suction pipe drawing liquid
Inner wall influences the experimental results such as heart contraction tension measurement；Cardiac output can only be detected, Testing index is single, is unfavorable for polygonal
Spend analysis of cardiac physiological function.
Invention content
Purpose of the utility model is to overcome the above-mentioned shortcomings and provide a kind of preparation of specimen simply, convenient for changing liquid, is suitable for
Synchronous detection cardiac mechanical shrinks experimental teaching and scientific research amphibian Perfused isolated heart device with electrophysiological function.
To achieve the goals above, the utility model adopts the following technical scheme that.
The utility model amphibian Perfused isolated heart device, pedestal are equipped with holder A and holder B, including artery is inserted
Pipe and venous cannula.The arterial cannulation combined by triple valve A connection arterial cannulations trunk, aorta insertion piece and drain pipe and
At the outlet end of the arterial cannulation trunk is connected with bend pipe and liquid droping port in turn, and the end of the drain pipe is connected with three
Port valve B；The venous cannula is composed of triple valve C connections liquid storage tank, posterior vena cava insertion piece and liquid-feeding tube, the storage
The side of the outer wall of liquid pool is equipped with side pipe, and the other side is equipped with the fixed link being fixed on holder A, the end connection of the liquid-feeding tube
There is triple valve D；The triple valve A and triple valve C are connected with each other, and the arterial cannulation trunk passes through side pipe, and the artery is made to insert
Pipe and venous cannula are combined into one.
The utility model amphibian Perfused isolated heart device further includes being arranged on the holder A, positioned at described solid
Note dropping electrode above fixed pole, the note dropping electrode include the note dropping electrode bar being fixed on the holder A, are separately positioned on institute
The note dropping electrode head and signal input part at note dropping electrode bar both ends are stated, the note dropping electrode head is located at the top of the liquid storage tank.
The utility model amphibian Perfused isolated heart device further includes the electrocardio leading electrode being arranged on holder B,
The electrocardio leading electrode includes electrocardio leading electrode bar, the electrocardio leading electrode in described electrocardio leading electrode bar one end is arranged
Signal input part in the electrocardio leading electrode bar other end is arranged in head and heart outer wall liquid-feeding tube, and the heart outer wall adds
Liquid pipe one end is equipped with triple valve E.
The end of the tapping side of the bend pipe is in closed, and the side wall of end is equipped with fluid hole.
The end of the aorta insertion piece and posterior vena cava insertion piece is equipped with the groove for fixing ligature.
The internal diameter of the bend pipe, aorta insertion piece and posterior vena cava insertion piece is all higher than 1.1 mm.
The beneficial effects of the utility model, when due to each component of perfusion device main body by insert section formula connect, make from
The aorta insertion piece and posterior vena cava insertion piece in device need to be only unloaded when systemic heart perfusion sample, be inserted into corresponding blood vessel, then
It is combined in device entirety, to make intubation become simple, is convenient for one man operation.Second is that being inserted with the drain pipe and vein of arterial cannulation
The liquid-feeding tube of pipe is conveniently replaceable perfusate, avoids being occurred with the phenomenon that influencing detection data stability when suction pipe liquid feeding and drain.
Three are provided with note dropping electrode, and note dropping electrode is matched with PowerLab or BL-420 biological functional systems, convenient for calculating
The cardiac output of random time point removes the inconvenience that conventional method visually remembers drop from.Fourth, electrocardio leading electrode is equipped with outside heart
Wall liquid-feeding tube can soak heart outer wall at any time, and not influence to detect electrocardio.Five are adapted for synchronous dynamic observation cardiac output, the heart
The dynamic change of a variety of parameters of left ventricular function such as flesh shrink tension and electrocardio.
Description of the drawings
Fig. 1 is the utility model amphibian Perfused isolated heart apparatus structure schematic diagram.
Fig. 2 is the utility model amphibian Perfused isolated heart device use state diagram.
In figure：1- tonotransducers, 2- tension lines, 3- frog heart clips, 4- isolated hearts, 5- aorta insertion pieces, 6- threeways
Valve A, 7- side pipe, 8- arterial cannulation trunks, 9- bend pipes, 10- liquid droping ports, 11- remember that dropping electrode head, 12- remember that dropping electrode bar, 13- are bis-
Recessed folder A, 14- double concave clips B, 15- remember dropping electrode signal input part, 16- crocodile clips A, 17- double concave clip C, 18- fixed link, 19- storages
Liquid pool, 20- triple valves C, 21- holder A, 22- liquid-feeding tube, 23- triple valves D, 24- syringe A, 25- syringe B, 26- triple valve
B, 27- drain pipe, 28- posterior vena cava insertion pieces, 29- electrocardio leading electrode heads, 30- heart outer wall liquid-feeding tubes, 31- triple valve E,
32- syringes C, 33- holder B, 34- double concave clip D, 35- electrocardio leading electrode bar, 36- electrocardio leading electrode signal input parts,
37- crocodile clips B, 38- pedestal.
Specific implementation mode
It elaborates below in conjunction with the accompanying drawings to the preferred embodiments of the present invention.
As shown in Figure 1, the utility model amphibian Perfused isolated heart device, pedestal carries holder A21 and holder
B33.The utility model includes arterial cannulation and venous cannula, the arterial cannulation by triple valve A6 connection arterial cannulations trunk 8,
Aorta insertion piece 5 and drain pipe 27 are composed, and the outlet end of the arterial cannulation trunk 8 is connected with bend pipe 9 and drop in turn
The end of liquid mouth 10, the drain pipe 27 is connected with triple valve B26.The end of the tapping side of the bend pipe 9 is in closed,
The side wall of end is equipped with fluid hole, and liquid is made to flow on 10 madial wall of liquid droping port.The venous cannula is connected by triple valve C20
Liquid storage tank 19, posterior vena cava insertion piece 28 and liquid-feeding tube 22 are composed, and the side of the outer wall of the liquid storage tank 19 is equipped with side pipe
7, the other side is equipped with the fixed link 18 being fixed on holder A21, and the end of the liquid-feeding tube 22 is connected with triple valve D23；It is described
Triple valve A6 and triple valve C20 are connected with each other, and the arterial cannulation trunk 8 passes through side pipe 7.Due to each component of perfusion device main body
It is connected by inserting section formula, the aorta insertion piece and posterior vena cava that need to be only unloaded when making Perfused isolated heart sample in device are inserted
Enter part, be inserted into corresponding blood vessel, in recombinant to device entirety, to make intubation become simple, is convenient for one man operation.Meanwhile with dynamic
The drain pipe of arteries and veins intubation and the liquid-feeding tube of venous cannula are conveniently replaceable perfusate, avoid with influence detection when suction pipe liquid feeding and drain
The phenomenon that data stability, occurs.
The utility model amphibian Perfused isolated heart device further includes being arranged on the holder A21 positioned at described
The note dropping electrode of 18 top of fixed link.The note dropping electrode includes the note dropping electrode bar 12 being fixed on the holder A21, difference
Note dropping electrode head 11 and signal input part 15 at 12 both ends of note dropping electrode bar is set.Remember dropping electrode and PowerLab or
BL-420 biological functional systems match, and convenient for calculating the cardiac output of random time point, it is visual to remove conventional method from
Remember the inconvenience of drop.
The utility model amphibian Perfused isolated heart device includes the electrocardio guiding electricity being arranged on holder B33 again
Pole.The electrocardio leading electrode includes electrocardio leading electrode bar 35, the electrocardio in described 35 one end of electrocardio leading electrode bar is arranged
The signal input part in 35 other end of electrocardio leading electrode bar is arranged in leading electrode head 29 and heart outer wall liquid-feeding tube 30
36, described 30 one end of heart outer wall liquid-feeding tube is equipped with triple valve E31.Since electrocardio leading electrode is equipped with heart outer wall liquid feeding
Pipe, can soak heart outer wall at any time, and not influence to detect electrocardio.
The end of the aorta insertion piece 5 and posterior vena cava insertion piece 28 is equipped with the groove for fixing ligature.
The internal diameter of the bend pipe 9, aorta insertion piece 5 and posterior vena cava insertion piece 28 is all higher than 1.1 mm.
The operation principle of the utility model is：
As shown in Fig. 2, by after arterial cannulation and venous cannula each component grafting combination, with double concave clip C17 by fixed link 18
It is fixed on holder A21, opens triple valve A6 and triple valve C20, close triple valve D23 and triple valve B26, add to liquid storage tank 19
Enter ringer solution, and closes triple valve C20 when liquid is flowed out by triple valve C20 from posterior vena cava insertion piece 28.
An amphibian toad or the frog are taken, brain is ruined and ruins marrow, frog board is fixed, and exposure heart takes from the perfusion device of combination
Lower posterior vena cava insertion piece 28, and it is inserted into atrium dextrum through posterior vena cava, perfusion ringer solution to liquid is smooth in intracardiac outflow
When heart bleaches, aorta insertion piece 5 is removed, and it is inserted into arterious cone through aorta sinistra, again perfusion ringer solution to stream
Go out smooth, heart is in vitro, and aorta insertion piece 5 and posterior vena cava insertion piece 28 are connected respectively to triple valve A6 and triple valve
On C20, triple valve C20 is opened, so that the ringer solution of liquid storage tank 19 is flowed into isolated heart 4 through posterior vena cava insertion piece 28, adjusts master
The angle of artery insertion piece 5 and posterior vena cava insertion piece 28 makes in isolated heart 4 liquid through aorta insertion piece 5, arterial cannulation
Trunk 8, bend pipe 9 and liquid droping port 10 flow into liquid storage tank 19, form one-way circulation flowing.
Tonotransducer 1 is fixed on to the top of holder A21 with double concave clip B14,1 signal input part of tonotransducer is connected
Tension is recorded on to the respective channel of PowerLab or BL-420 biological functional systems, isolated heart is clamped with frog heart clip 3
At 4 apex of the heart, adjustment aorta insertion piece 5 and posterior vena cava insertion piece 28 keep the apex of the heart upward, adjust the tension of tension lines 2, make
Isolated heart 4 keeps the easypro contracting state of nature.
Note dropping electrode bar 12 is fixed on double concave clip A13 between the tonotransducer 1 of holder A21 and fixed link 18, makes note
Dropping electrode head 11 is between liquid droping port 10 and liquid storage tank 19, and drips are on note dropping electrode head 11, note dropping electrode signal input
After end 15 is clamped with crocodile clip A16, it is connected in the respective channel of PowerLab or BL-420 biological functional systems and records drop
Number.
Electrocardio leading electrode bar 35 is fixed on double concave clip D34 on holder B33, makes the positive and negative anodes of electrocardio leading electrode head 29
Contact the apex of the heart and atrium dextrum lateral wall respectively, electrocardio leading electrode signal input part 36 with crocodile clip B37 be connected to PowerLab or
Electrocardio is recorded in the respective channel of BL-420 biological functional systems, is opened triple valve E31, is passed through liquid feeding with syringe C32
The outer wall of 30 liquid injection and humidity isolated heart 4 of pipe.
When replacing perfusate, triple valve D23 is opened, with the liquid in syringe A24 extraction liquid storage tanks 19, opens triple valve
B26 closes triple valve B26, injects fresh Ren Shi from triple valve D23 after 8 liquid of syringe B25 extraction arterial cannulations trunk
Liquid or liquid are complete to liquid circulation, and the liquid level in liquid storage tank 19 closes triple valve D23 when reaching specified altitude assignment.
The above is only the explanation to the preferred embodiments of the present invention, not makees any form to the utility model
On limitation, every according to the technical essence of the utility model any simple modification made to the above embodiment, equivalent variations
With modification, technical solutions of the utility model range is belonged to.
Claims (6)
1. a kind of amphibian Perfused isolated heart device, pedestal（38）It is equipped with holder A（21）With holder B（33）, feature
It is, including arterial cannulation and venous cannula, the arterial cannulation is by triple valve A（6）Connect arterial cannulation trunk（8）, actively
Arteries and veins insertion piece（5）And drain pipe（27）It is composed, the arterial cannulation trunk（8）Outlet end be connected with bend pipe in turn（9）
And liquid droping port（10）, the drain pipe（27）End be connected with triple valve B（26）；The venous cannula is by triple valve C
（20）Connect liquid storage tank（19）, posterior vena cava insertion piece（28）And liquid-feeding tube（22）It is composed, the liquid storage tank（19）It is outer
The side of wall is equipped with side pipe（7）, the other side is equipped with and is fixed on holder A（21）On fixed link（18）, the liquid-feeding tube（22）'s
End is connected with triple valve D（23）；The triple valve A（6）With triple valve C（20）It is connected with each other, the arterial cannulation trunk（8）
Across side pipe（7）.
2. amphibian Perfused isolated heart device according to claim 1, it is characterised in that：Further include being arranged described
Holder A（21）Above, it is located at the fixed link（18）The note dropping electrode of top, the note dropping electrode include being fixed on the holder A
（21）On note dropping electrode bar（12）, be separately positioned on the note dropping electrode bar（12）The note dropping electrode head at both ends（11）And signal
Input terminal（15）, the note dropping electrode head（11）Positioned at the liquid storage tank（19）Top.
3. amphibian Perfused isolated heart device according to claim 1, it is characterised in that：Further include being arranged described
Holder B（33）On electrocardio leading electrode, the electrocardio leading electrode includes electrocardio leading electrode bar（35）, be arranged in the heart
Electric leading electrode bar（35）The electrocardio leading electrode head of one end（29）With heart outer wall liquid-feeding tube（30）, setting draw in the electrocardio
Conductive pole bar（35）The signal input part of the other end（36）, the heart outer wall liquid-feeding tube（30）One end is equipped with triple valve E（31）.
4. amphibian Perfused isolated heart device according to claim 1, it is characterised in that：The bend pipe（9）Go out
The end of liquid side is in closed, and the side wall of end is equipped with fluid hole.
5. amphibian Perfused isolated heart device according to claim 1, it is characterised in that：The aorta insertion piece
（5）With posterior vena cava insertion piece（28）End be equipped with the groove for fixing ligature.
6. amphibian Perfused isolated heart device according to claim 1 or 5, it is characterised in that：The bend pipe（9）、
Aorta insertion piece（5）With posterior vena cava insertion piece（28）Internal diameter be all higher than 1.1 mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820357075.9U CN207923885U (en) | 2018-03-16 | 2018-03-16 | Amphibian Perfused isolated heart device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820357075.9U CN207923885U (en) | 2018-03-16 | 2018-03-16 | Amphibian Perfused isolated heart device |
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| Publication Number | Publication Date |
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| CN207923885U true CN207923885U (en) | 2018-09-28 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201820357075.9U Active CN207923885U (en) | 2018-03-16 | 2018-03-16 | Amphibian Perfused isolated heart device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108196042A (en) * | 2018-03-16 | 2018-06-22 | 延边大学 | Amphibian Perfused isolated heart device |
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2018
- 2018-03-16 CN CN201820357075.9U patent/CN207923885U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108196042A (en) * | 2018-03-16 | 2018-06-22 | 延边大学 | Amphibian Perfused isolated heart device |
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