CN110320351A - Isolated frog heart perfusion experiment instructional device - Google Patents
Isolated frog heart perfusion experiment instructional device Download PDFInfo
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- CN110320351A CN110320351A CN201910772534.9A CN201910772534A CN110320351A CN 110320351 A CN110320351 A CN 110320351A CN 201910772534 A CN201910772534 A CN 201910772534A CN 110320351 A CN110320351 A CN 110320351A
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- head
- storage tank
- liquid storage
- arterial cannulation
- liquid
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- 238000002474 experimental method Methods 0.000 title claims abstract description 15
- 230000010412 perfusion Effects 0.000 title claims abstract description 14
- 239000007788 liquid Substances 0.000 claims abstract description 30
- 238000002627 tracheal intubation Methods 0.000 abstract description 7
- 230000000747 cardiac effect Effects 0.000 abstract description 3
- 238000002360 preparation method Methods 0.000 abstract description 3
- 239000003146 anticoagulant agent Substances 0.000 abstract description 2
- 229940127219 anticoagulant drug Drugs 0.000 abstract description 2
- 238000000034 method Methods 0.000 description 8
- XEEYBQQBJWHFJM-UHFFFAOYSA-N iron Substances [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 7
- 210000000709 aorta Anatomy 0.000 description 5
- 239000008280 blood Substances 0.000 description 5
- 210000004369 blood Anatomy 0.000 description 5
- 239000012891 Ringer solution Substances 0.000 description 4
- 229910052742 iron Inorganic materials 0.000 description 4
- 241000132931 Batrachia Species 0.000 description 2
- 210000001367 artery Anatomy 0.000 description 2
- 230000023555 blood coagulation Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 230000004217 heart function Effects 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 238000009833 condensation Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000003205 diastolic effect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 230000010247 heart contraction Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000033764 rhythmic process Effects 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
-
- G—PHYSICS
- G09—EDUCATION; CRYPTOGRAPHY; DISPLAY; ADVERTISING; SEALS
- G09B—EDUCATIONAL OR DEMONSTRATION APPLIANCES; APPLIANCES FOR TEACHING, OR COMMUNICATING WITH, THE BLIND, DEAF OR MUTE; MODELS; PLANETARIA; GLOBES; MAPS; DIAGRAMS
- G09B23/00—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes
- G09B23/28—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes for medicine
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- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Business, Economics & Management (AREA)
- Biomedical Technology (AREA)
- Mathematical Analysis (AREA)
- Mathematical Optimization (AREA)
- Mathematical Physics (AREA)
- Pure & Applied Mathematics (AREA)
- Algebra (AREA)
- Educational Administration (AREA)
- Educational Technology (AREA)
- Theoretical Computer Science (AREA)
- Medical Informatics (AREA)
- Computational Mathematics (AREA)
- Hematology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a kind of isolated frog heart perfusion experiment instructional devices, including liquid storage tank, arterial cannulation head and fixed link, a side pipe is arranged in the apical lateral of the liquid storage tank, one end of bar is plugged and fixed in the side pipe, the lower end of the liquid storage tank is plugged into arterial cannulation head, and a side hook and a liquid-feeding tube is arranged on the side wall of the arterial cannulation head.The present invention has the characteristics that easy intubation, anticoagulant, is fixed easily, convenient for changing liquid, and the stability and reliability of Perfused isolated heart preparation of specimen success rate and cardiac systolic function test experience data can be greatly improved.
Description
Technical field
A kind of of the invention and a kind of life science related experiment teaching device, and in particular to isolated frog heart perfusion experiment religion
Learn device.
Background technique
Can intuitively observe the activity of heart using Perfused isolated heart system, study heart systolic and diastolic function, heart rate and
The influence of rhythm and pace of moving things variation and various humoral factors to cardiac function, is one of the important means of research cardiac function, answers extensively
For teaching and scientific researches such as medicine, biology, pharmacy.Single pipe method (Straub ' s method) heart carried out using batrachia isolated heart
Perfusion experiment requires simply experimental situation, and whole experiment process is not required to constant temperature and oxygen supply equipment, is widely used in life science
Experimental teaching.But traditional single pipe method has more in practical applications due to the design feature of frog heart cannula and frog heart pipe clamp
Drawback.Because frog heart cannula is glassware, it is easily damaged in experimentation；Opposite batrachia aorta, frog heart cannula tip compared with
It is thick blunt, it is not easy to be inserted into ventricle, keeps cardiac perfusion preparation of specimen difficult；Blood is inconvenient in flushing pipe when intubation, is easy to cause blood coagulation
Obstruction increases intubation defeat probability；Since frog heart pipe clamp clamps not strongly, heart contraction tension detection is easily influenced in experimentation；
It needs to be added or extract the liquid in arterial cannulation with suction pipe in experimentation, is easy touching intubation inner wall, influences experimental data
Accuracy and reliability.
Summary of the invention
The object of the present invention is to overcome the deficiencies of the prior and provides a kind of easily intubation, anticoagulant, Yi Gu
Determine, convenient for changing liquid, can be adapted to the isolated frog heart perfusion experiment instructional device of science detection cardiac systolic function.
In order to solve the above technical problem, the present invention provides following technical solutions:
A kind of isolated frog heart perfusion experiment instructional device, including liquid storage tank, arterial cannulation head and fixed link, the liquid storage tank
A side pipe is arranged in apical lateral, and one end of bar is plugged and fixed in the side pipe, and the lower end of the liquid storage tank is plugged into artery
It is intubated head, a side hook and a liquid-feeding tube are set on the side wall of the arterial cannulation head.
Further, the internal diameter of the liquid storage tank is 9.0-12.0 mm.
Further, the tip internal diameter of the arterial cannulation head is 1.0 mm or more, and outer diameter is 1.6 mm or less.
Compared with prior art, the beneficial effects of the present invention are: 1. arterial cannulation head internal diameter becomes larger, outer diameter becomes smaller, and holds very much
Easily by aorta petal, even beginner is also easy to grasp intubation technique.2. can be with being connected on liquid-feeding tube in catheterization procedure
Syringe rinses the blood flowed into arterial cannulation head from ventricle at any time, can effectively prevent blood coagulation.3. due to each portion of apparatus main body
Part socket connection only need to will be connected with the arterial cannulation head of liquid storage tank through aorta sinistra when making Perfused isolated heart sample
It is inserted into intra-ventricle, then is inserted into fixed link by the side pipe on liquid storage tank and can firmly fix heart lung preparation, side easy to operate
Just.4. suction pipe liquid feeding and drain can be removed from liquid-feeding tube, it is easily changed perfusate.5. isolated frog heart perfusion experiment instructional device makes
With tolerance external force material, it is hardly damaged.
Detailed description of the invention
Fig. 1 is structural schematic diagram of the invention.
Fig. 2 is use state diagram of the invention.
In Fig. 1, Fig. 2: 1- tonotransducer, 2- tension lines, 3- liquid storage tank, 4- arterial cannulation head, the side 5- hook, the in vitro frog of 6-
The heart, 7- frog heart clip, 8- pulley, 9- double concave clip, 10- side pipe, 11- double concave clip, 12- fixed link, 13- liquid-feeding tube, 14- double concave clip,
15- iron stand, 16- syringe.
Specific embodiment
Invention is further described in detail with reference to the accompanying drawings and detailed description.
As shown in Figure 1, a kind of isolated frog heart perfusion experiment instructional device, including liquid storage tank 3, arterial cannulation head 4 and fixation
Bar 12, the internal diameter of the liquid storage tank 3 are 9.0-12.0 mm, and a side pipe 10 is arranged in the apical lateral of liquid storage tank 3, described
One end of bar 12 is plugged and fixed in side pipe 10, and the lower end of the liquid storage tank 3 is plugged into arterial cannulation head 4, the point of arterial cannulation head 4
End internal diameter is 1.0 mm or more, outer diameter be 1.6 mm hereinafter, be arranged on the side wall of arterial cannulation head 4 a side hook 5 and one plus
Liquid pipe 13.This isolated frog heart perfusion experiment instructional device is made using breakage resistant luer material.
The working principle of the invention: as shown in Fig. 2, tonotransducer 1 is fixed on the top of iron stand 15 with double concave clip 9,
Fixed link 12 is fixed on the middle section of iron stand 15 with double concave clip 11, and pulley 8 is fixed on the lower section of iron stand 15 with double concave clip 14；It will
Arterial cannulation head 4 is inserted into 3 lower end of liquid storage tank, after extracting ringer solution with syringe 16, from liquid-feeding tube 13, and through arterial cannulation head 4, to
Injection is appropriate in liquid storage tank 3；A toad or the frog are taken, brain is ruined and ruins marrow, frog board is fixed, and exposure heart will be connected with the artery of liquid storage tank 3
It is intubated head 4 and is inserted into ventricle through aorta sinistra and arterious cone, left and right aorta is pricked with knot, and after side hook 5 is fixed, by heart
In vitro, isolated frog heart sample is made, the blood assembled in intubation head is rinsed with syringe 16 at any time in catheterization procedure, with anti-condensation
Blood after having made sample, topples over liquid storage tank and includes blood ringer solution, inject fresh ringer solution.Isolated frog heart sample is passed through into side pipe
10 are inserted into fixed link 12；By the frog heart clip 7 for connecting tension lines 2 around the apex of the heart for after pulley 8, clamping isolated frog heart 6 at, adjustment
The tension of tension lines 2 makes isolated frog heart 6 keep the easypro contracting state of nature；1 signal input part of tonotransducer be connected to PowerLab or
Tension curve is recorded in the respective channel of BL-420 biological functional system；When replacing perfusate, is extracted out and stored up with syringe 16
Liquid in liquid pool 3 reinjects fresh ringer solution or medical fluid to specified liquid level.
The above is only the preferred embodiments of the present invention, and is not intended to limit the present invention in any form,
Any simple modification made to the above embodiment according to the technical essence of the invention, equivalent variations and modification, belong to
In the range of technical solution of the present invention.
Claims (3)
1. a kind of isolated frog heart perfusion experiment instructional device, it is characterised in that including liquid storage tank, arterial cannulation head and fixed link, institute
The apical lateral of the liquid storage tank stated is arranged a side pipe, and the side pipe is plugged and fixed one end of bar, under the liquid storage tank
End is plugged into arterial cannulation head, and a side hook and a liquid-feeding tube is arranged on the side wall of the arterial cannulation head.
2. the isolated frog heart perfusion experiment instructional device as described in claim 1, it is characterised in that the internal diameter of the liquid storage tank is
9.0-12.0 mm。
3. the isolated frog heart perfusion experiment instructional device as described in claim 1, it is characterised in that the point of the arterial cannulation head
End internal diameter is 1.0 mm or more, and outer diameter is 1.6 mm or less.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910772534.9A CN110320351A (en) | 2019-08-21 | 2019-08-21 | Isolated frog heart perfusion experiment instructional device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910772534.9A CN110320351A (en) | 2019-08-21 | 2019-08-21 | Isolated frog heart perfusion experiment instructional device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110320351A true CN110320351A (en) | 2019-10-11 |
Family
ID=68126360
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910772534.9A Withdrawn CN110320351A (en) | 2019-08-21 | 2019-08-21 | Isolated frog heart perfusion experiment instructional device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110320351A (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2582078Y (en) * | 2002-08-28 | 2003-10-22 | 王庆山 | Apparatus for counting drops of in vitro frog heart perfusion |
| CN104825233A (en) * | 2015-05-13 | 2015-08-12 | 南阳医学高等专科学校 | Improved experimental device for frog heart perfusion |
| CN206271254U (en) * | 2016-10-31 | 2017-06-20 | 重庆三峡医药高等专科学校 | A kind of frog heart cannula |
| CN207238026U (en) * | 2017-06-02 | 2018-04-17 | 长沙医学院 | A kind of frog heart perfusion experiment intubation |
| CN108196042A (en) * | 2018-03-16 | 2018-06-22 | 延边大学 | Amphibian Perfused isolated heart device |
| CN210665754U (en) * | 2019-08-21 | 2020-06-02 | 延边大学 | In-vitro frog heart perfusion experiment teaching device |
-
2019
- 2019-08-21 CN CN201910772534.9A patent/CN110320351A/en not_active Withdrawn
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2582078Y (en) * | 2002-08-28 | 2003-10-22 | 王庆山 | Apparatus for counting drops of in vitro frog heart perfusion |
| CN104825233A (en) * | 2015-05-13 | 2015-08-12 | 南阳医学高等专科学校 | Improved experimental device for frog heart perfusion |
| CN206271254U (en) * | 2016-10-31 | 2017-06-20 | 重庆三峡医药高等专科学校 | A kind of frog heart cannula |
| CN207238026U (en) * | 2017-06-02 | 2018-04-17 | 长沙医学院 | A kind of frog heart perfusion experiment intubation |
| CN108196042A (en) * | 2018-03-16 | 2018-06-22 | 延边大学 | Amphibian Perfused isolated heart device |
| CN210665754U (en) * | 2019-08-21 | 2020-06-02 | 延边大学 | In-vitro frog heart perfusion experiment teaching device |
Non-Patent Citations (1)
| Title |
|---|
| 陶媛媛等: "单管法离体心脏灌流实验教学改进", 实验室研究与探索, vol. 37, no. 12, 31 December 2018 (2018-12-31), pages 201 - 205 * |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20191011 |