CN215227923U

CN215227923U - Direct implantation type open micro-perfusion probe

Info

Publication number: CN215227923U
Application number: CN202121067251.3U
Authority: CN
Inventors: 王斌; 杨慧; 邰晨旭; 刘硕
Original assignee: Nanjing Drum Tower Hospital
Current assignee: Nanjing Drum Tower Hospital
Priority date: 2021-05-19
Filing date: 2021-05-19
Publication date: 2021-12-21
Anticipated expiration: 2031-05-19

Abstract

The utility model discloses a direct implantation type open micro-perfusion probe, which comprises a micro-perfusion capillary tube and a solid guide needle; one end of the solid guide needle is a pointed end, and the other end of the solid guide needle is detachably connected with the micro-irrigation capillary; the middle section of the micro-irrigation capillary tube is provided with a micropore; the micropores are through holes; the solid guide needle is made of stainless steel material; the micro-irrigation capillary tube is spirally connected with the solid guide needle; at the connecting part of the micro-irrigation capillary tube and the solid guide needle, the micro-irrigation capillary tube is provided with an internal thread, and the solid guide needle is provided with an external thread; the internal thread corresponds to the external thread. The utility model adopts the solid guide needle to draw the micro-perfusion capillary tube to directly implant into the tissue, carries out open micro-perfusion sampling, has simple structure, and improves the sampling efficiency of protein, polypeptide and lipophilic substances; the diameter of the solid guide needle is slightly smaller than that of the micro perfusion capillary, so that the damage to the organism tissue is reduced; can dismantle to set up and make the probe dismouting in a flexible way, used repeatedly reduces the sample cost.

Description

Direct implantation type open micro-perfusion probe

Technical Field

The utility model belongs to the technical field of medical instrument, concretely relates to open probe that fills a little of direct implanted.

Background

The open micro perfusion sampling is carried out on the premise that the probe is implanted into a target tissue, before the open micro perfusion probe is implanted, a stainless steel pointed catheter with a diameter larger than that of the open micro perfusion probe is firstly penetrated into the tissue, then the probe is penetrated into the catheter, and then the catheter is removed to leave the probe in the tissue. In addition, in the existing microdialysis technology, a microdialysis probe inserted into a living tissue is perfused under an unbalanced condition, and substances are diffused along a concentration gradient, so that the analyzed substances are diffused into a dialysis tube through a dialysis membrane and are continuously carried out by perfusion liquid continuously flowing in the dialysis tube, and the purpose of sampling the living tissue is achieved.

However, the micro-dialysis technology employs a dialysis membrane, which can cause the molecular weight cut-off of the dialysis membrane during the dialysis exchange process, and the problems of membrane contamination and blood coagulation cannot be avoided during the dialysis process, so that the sampling result is easily affected. In the process of implanting the probe, the use of the catheter with a larger outer diameter inevitably damages tissues, so that the sampling can be carried out after a longer balance time to obtain reliable concentration information of interstitial fluid components. On the other hand, when the probe is implanted by using the catheter, in order to prevent the port of the probe from being blocked in the implantation process, the two ends of the probe are generally designed to be sealed, and the sealed part is cut off when the probe is implanted into tissues for use, so that the expensive probe can be used only for 1 time, the cost is high, and the popularization and the application of the technology are hindered.

SUMMERY OF THE UTILITY MODEL

In view of this, the utility model discloses it is expected to provide an open little perfusion probe of direct implantation formula, can reduce the damage that the probe implantation process caused organism tissue, simultaneously can also used repeatedly, reduce use cost.

In order to achieve the above purpose, the technical scheme of the utility model is realized like this:

a direct implantation type open micro-perfusion probe comprises a micro-perfusion capillary tube and a solid guide needle; one end of the solid guide needle is a pointed end, and the other end of the solid guide needle is detachably connected with the micro-irrigation capillary; the micro-perfusion capillary tube is provided with a micropore in the middle section, and the micropore is a through hole to form an open micro-perfusion probe.

The micro-irrigation capillary tube and the solid guide needle are detachably connected, so that the probe can be flexibly disassembled and assembled and can be repeatedly used, and the cost is effectively reduced.

The solid guide needle is used for drawing the micro-perfusion capillary to implant into tissues to carry out open micro-perfusion sampling, the structure is simple, the operation is convenient, the diameter, the number or the arrangement range of the micropores can be adjusted, and the open design greatly improves the sampling efficiency of protein, polypeptide, lipophilic substances and the like.

Further, the solid guide needle is made of stainless steel.

Further, the micro perfusion capillary is spirally connected with a solid guide needle; at the connecting part of the micro-irrigation capillary tube and the solid guide needle, the micro-irrigation capillary tube is provided with an internal thread, and the solid guide needle is provided with an external thread; the internal thread corresponds to the external thread.

Furthermore, two ends of the micro-irrigation capillary are communicated with a push-pull pump, and the push-pull pump comprises a pressure side and a suction side.

After the solid guide needle is separated from the micro-irrigation capillary tube, two ends of the micro-irrigation capillary tube are connected with a push-pull pump, and the push-pull pump comprises a pressure side and a suction side; the pressure side and suction side operating efficiencies remain consistent.

Here, the pressure side of the push-pull pump injects perfusion liquid into the micro-perfusion capillary under pressure to perform material exchange at the micro-pores, and the suction side of the push-pull pump collects the perfusion liquid subjected to material exchange through suction force so as to facilitate the subsequent detection work.

Here, the perfusion rate on the pressure side of the push-pull pump and the collection rate on the suction side are substantially or completely consistent, so that the material collection operation is smoothly and orderly performed.

Furthermore, the micropores are through holes with the diameter of 0.05mm, and are uniformly distributed into a micropore array in the middle section of the micro-irrigation capillary tube, wherein the micropore array is a rectangular array or a square array; the axial length of the micro-pore array on the micro-irrigation capillary tube is 13 mm-18 mm; the micropores are formed by laser drilling.

Here, the micropores are provided as through holes, facilitating the exchange of substances between the perfusion fluid and the interstitial fluid.

Furthermore, the number of the micropores is 100, and the micropores are uniformly distributed in the middle section of the micro-irrigation capillary in a 10 × 10 square array.

Here, the micro-wells may be arranged in a 2-component arrangement in a 10 × 5 rectangular array, and the center axis of the micro-irrigation capillary is a symmetry axis, and is axisymmetrically arranged on the micro-irrigation capillary.

Further, the micro-perfusion capillary is a polyimide capillary.

Further, the inner diameter of the micro perfusion capillary tube is 0.35-0.45 mm, the outer diameter is 0.50-0.60 mm, and the length is 18-23 cm; the size of the internal thread of the micro-perfusion capillary tube is 0.125mm in pitch, 0.500-0.600 mm in major diameter, 0.419-0.445 mm in middle diameter and 0.380-0.415 mm in minor diameter.

Here, the micro perfusion capillary may have an inner diameter of 0.40mm and an outer diameter of 0.55 mm.

Here, the internal thread of the micro-irrigation capillary may have a pitch of 0.125mm, a major diameter of 0.500mm, a median diameter of 0.430mm, and a minor diameter of 0.400 mm.

Further, the diameter of the solid guide needle is 0.49mm, and the length of the solid guide needle is 3-5 cm; the size of the external thread of the solid guide needle is 0.125mm in pitch, 0.480-0.500 mm in major diameter, 0.393-0.419 mm in intermediate diameter and 0.332-0.360 mm in minor diameter.

Here, the solid introducer needle external thread may have a pitch of 0.125mm, a major diameter of 0.490mm, a median diameter of 0.410mm, and a minor diameter of 0.360 mm.

The diameter of the solid guide needle is slightly smaller than the outer diameter of the micro-irrigation capillary, so that the probe can be conveniently and directly implanted, the damage to organism tissues when the probe is implanted is reduced, and the balance time before sampling is shortened.

Further, the inner diameter of the micro perfusion capillary is 0.40mm, and the outer diameter is 0.55 mm; the size of the internal thread of the micro-perfusion capillary is 0.125mm, the major diameter is 0.500mm, the middle diameter is 0.430mm, and the minor diameter is 0.400 mm; the external thread of the solid guide needle has the size of 0.125mm of thread pitch, 0.490mm of major diameter, 0.410mm of intermediate diameter and 0.360mm of minor diameter.

Here, the size of the internal thread of the micro-irrigation capillary is referenced to the size of the internal thread S0.5 in small thread limit size (GB/T15054.5-94) in the national standard, and the size of the external thread of the solid guide pin is referenced to the size of the external thread S0.5 in small thread limit size (GB/T15054.5-94) in the national standard.

The operation method comprises the following steps:

1) tightly connecting the micro-irrigation capillary tube with a solid guide needle, inserting the solid guide needle tip into a target tissue, penetrating the target tissue, penetrating out of the body surface, and continuously drawing the solid guide needle to enable the micro-pore array of the micro-irrigation capillary tube to be positioned in the target tissue;

2) separating the solid guide needle from the micro-irrigation capillary tube, respectively connecting the two ends of the micro-irrigation capillary tube with the pressure side and the suction side of a push-pull pump, setting the pump speed, and operating the push-pull pump;

3) the pressure side of the push-pull pump continuously injects perfusion liquid into the micro-injection capillary, the perfusion liquid and interstitial liquid components at the micropores generate convection, and the suction side of the push-pull pump is matched with the speed of the perfusion liquid injected by the pressure side to orderly collect the perfusion liquid which generates material exchange in the micro-injection capillary for the next detection and analysis.

Here, the use of a microporous array for material exchange without dialysis membrane perfusion allows for more reliable collection of high molecular weight proteins, polypeptides and lipophilic materials, avoids entrapment of materials by the dialysis membrane, and also avoids membrane fouling and clotting problems.

The perfusion liquid is buffer liquid or normal saline which is similar to interstitial liquid, and basically does not influence normal life activities of organisms, so that living body sampling is realized.

The direct implantation type open micro-perfusion probe can be applied to various tissues, and the change condition of the interstitial components of the target tissues of interest can be monitored in situ and on line through continuous sampling.

The utility model discloses beneficial effect as follows: 1) the utility model discloses a direct implantation formula open micro perfusion probe draws the micro perfusion capillary by solid guide needle and implants the tissue, carries out open micro perfusion biopsy body sample, simple structure, and the simple operation also can adjust micropore diameter, quantity or range, and this kind of open design has improved the sampling efficiency of protein, polypeptide and lipophilic material etc. greatly; 2) the solid guide needle used by the utility model has a diameter slightly smaller than the outer diameter of the micro-irrigation capillary tube, so that the probe can be conveniently and directly implanted, the damage to organism tissues when the probe is implanted is reduced, and the balance time before sampling is shortened; 3) the utility model discloses fill capillary and solid guide needle a little and can dismantle the setting of connection, make the probe dismouting in a flexible way, used repeatedly, effective reduce cost.

Drawings

Fig. 1 is a schematic perspective view of a direct implantation type open micro perfusion probe according to an embodiment of the present invention;

fig. 2 is an enlarged schematic view of a spiral structure of a direct implantation type open micro-perfusion probe according to an embodiment of the present invention;

fig. 3 is an enlarged schematic view of a microporous structure of a direct implantation type open micro-perfusion probe according to an embodiment of the present invention;

wherein, 1 is a micro-irrigation capillary, 2 is a solid guide needle, 3 is a micropore, 4 is a micro-irrigation capillary internal thread, 5 is a solid guide needle tip, and 6 is a solid guide needle external thread.

Detailed Description

In order to understand the features and technical contents of the present invention in more detail, the following description is given in conjunction with the accompanying drawings, which are only used for reference and not for limiting the present invention.

Fig. 1 is a schematic view of a three-dimensional structure of a direct implantation type open micro-perfusion probe according to an embodiment of the present invention, as shown in fig. 1, the present invention relates to a direct implantation type open micro-perfusion probe, which includes a micro-perfusion capillary 1 and a solid guide needle 2; one end of the solid guide needle 2 is a solid guide needle sharp head 5, and the other end is provided with a solid guide needle external thread 6; one end of the micro perfusion capillary 1 is provided with a micro perfusion capillary internal thread 4; the internal thread 4 of the micro-irrigation capillary corresponds to the external thread 6 of the solid guide needle, so that the solid guide needle 2 is in spiral connection with the micro-irrigation capillary 1; the middle section of the micro-perfusion capillary tube 1 is also provided with 80 micropores which are divided into 2 groups and are distributed on the micro-perfusion capillary tube 1 in an axisymmetric manner by using an 8 multiplied by 5 rectangular array and taking the central axis of the micro-perfusion capillary tube 1 as a symmetric axis.

Further, the micro-perfusion capillary 1 is a polyimide capillary.

Further, the solid introducer needle 2 is a solid stainless steel introducer needle.

Further, the micro-hole 3 is a through hole with a diameter of 0.05mm, forming an open micro-perfusion probe.

Here, the micropores 3 are provided as through holes to facilitate the exchange of substances between the perfusion fluid and the interstitial fluid.

Here, the micro-irrigation capillary 1 and the solid guide needle 2 are in spiral connection, so that the probe can be flexibly disassembled and assembled and can be repeatedly used, and the cost is effectively reduced.

Here, draw the capillary 1 of micro perfusion by the solid guide needle 2 and implant the tissue, sample by the open micro perfusion, simple in construction, easy and fast to operate, can also adjust the diameter of the micropore, quantity, or range of arrangement, the open design has greatly raised the sampling efficiency of protein, polypeptide and lipophilic substance, etc.

Further, after the solid guide needle 2 is separated from the micro-irrigation capillary tube 1, two ends of the micro-irrigation capillary tube 1 are communicated with a push-pull pump; the push-pull pump comprises a pressure side and a suction side; the pressure side and suction side operating efficiencies remain consistent.

Here, the push-pull pump is of the Chemyx Fusion 4000 type.

Further, the axial length of the micro-pore array on the micro-irrigation capillary 1 is 15 mm; the micro-holes 3 are formed by laser drilling.

Further, the micro perfusion capillary 1 may have an inner diameter of 0.40mm, an outer diameter of 0.55mm, and a length of 20 cm.

Furthermore, the thread 4 in the micro perfusion capillary has the pitch of 0.125mm, the major diameter of 0.500mm, the middle diameter of 0.430mm and the minor diameter of 0.400 mm.

Further, the solid introducer needle external thread 6 can have the thread pitch of 0.125mm, the major diameter of 0.490mm, the middle diameter of 0.410mm and the minor diameter of 0.360 mm.

Further, the solid introducer needle 2 has a diameter of 0.49mm and a length of 4 cm.

Further, the inner diameter of the micro perfusion capillary 1 is 0.40mm, and the outer diameter is 0.55 mm.

Here, the diameter of the solid guide needle 2 is slightly smaller than the outer diameter of the micro-irrigation capillary 1, so that the probe can be conveniently and directly implanted, the damage to organism tissues when the probe is implanted is reduced, and the balance time before sampling is shortened.

The operation method of using a direct implantation type open micro-perfusion probe to carry out in-situ open micro-perfusion sampling on the living body of the rat liver comprises the following steps:

1) anesthetizing the rat, fixing the rat in a supine lying mode, shaving hairs, disinfecting the upper abdomen, opening the abdominal cavity from a median incision, and exposing the liver;

2) the micro-perfusion capillary 1 and the solid guide needle 2 are spirally connected through the micro-perfusion capillary internal thread 4 and the solid guide needle external thread 6;

3) the sharp head 5 of the solid guide needle penetrates through the left middle lobe of the rat liver and then penetrates out of the body surface, and the solid guide needle 2 is continuously drawn to enable the micropore array area of the micro-irrigation capillary 1 to be positioned in the rat liver tissue;

4) unscrewing the threaded connection part, and taking down the solid guide needle 2 to separate the solid guide needle from the micro-irrigation capillary 1;

5) two ends of the micro-perfusion capillary 1 are respectively connected with a push-pull pump pressure side and a suction side, the pump speed is set, the perfusion liquid is continuously injected into the probe by the push-pull pump pressure side, the perfusion liquid and interstitial liquid components generate convection at the micropore, suction is generated from the suction side of the push-pull pump at the other end of the micro-perfusion capillary 1, and the perfusion liquid after the substance exchange is generated is collected for the detection and analysis of the next step.

The utility model can directly implant the micro-perfusion capillary into the tissue by the traction of the solid guide needle, carry out open micro-perfusion sampling, has simple structure, can also adjust the size and the distribution of the micro-pore array on the micro-perfusion capillary, and improves the sampling efficiency of protein, polypeptide and lipophilic substances; a solid guide needle with the diameter slightly smaller than the outer diameter of the micro-irrigation capillary is used, and the probe is directly implanted through threaded connection, so that the damage to biological tissues during the implantation of the probe is reduced, and the balance time before sampling is shortened; the detachable setting makes micro-irrigation capillary and solid guide needle can nimble dismouting, used repeatedly, reduce the sample cost.

The specific type of the above-mentioned devices is not limited and detailed, and the deep connection mode of the above-mentioned devices is not detailed, and can be understood by those skilled in the art as the common general knowledge.

The above description is only for the purpose of illustrating the preferred embodiments of the present invention and should not be taken as limiting the scope of the present invention, and any modifications, equivalent replacements, and improvements made within the spirit and principle of the present invention should be included in the scope of the present invention.

Claims

1. A direct implantation type open micro-perfusion probe is characterized by comprising a micro-perfusion capillary tube and a solid guide needle; one end of the solid guide needle is a pointed end, and the other end of the solid guide needle is detachably connected with the micro-irrigation capillary; the middle section of the micro-irrigation capillary tube is provided with a micropore; the micropores are through holes.

2. The direct implantation open micro infusion probe according to claim 1, wherein said solid guiding needle is made of stainless steel material.

3. The direct implantable open micro infusion probe according to claim 1, wherein said micro infusion capillary is helically connected to a solid guide pin; at the connecting part of the micro-irrigation capillary tube and the solid guide needle, the micro-irrigation capillary tube is provided with an internal thread, and the solid guide needle is provided with an external thread; the internal thread corresponds to the external thread.

4. The direct-implantation open micro-perfusion probe of claim 1, wherein the micro-perfusion capillary tube is connected to a push-pull pump at both ends; the push-pull pump includes a pressure side and a suction side.

5. The direct implantation type open micro-perfusion probe of claim 1, wherein the micro-holes are through holes with a diameter of 0.05mm, and are uniformly distributed in a micro-hole array in the middle section of the micro-perfusion capillary tube, wherein the micro-holes are rectangular array or square array; the axial length of the micro-pore array on the micro-irrigation capillary tube is 13 mm-18 mm; the micropores are formed by laser drilling.

6. The direct-implantation open micro-perfusion probe of claim 5, wherein the number of the micro-holes is 100, and the micro-holes are uniformly distributed in a 10 x 10 square array at the middle section of the micro-perfusion capillary.

7. The direct-implantation open micro perfusion probe of claim 1, wherein the micro perfusion capillary is a polyimide capillary.

8. The direct implantation type open micro perfusion probe according to claim 3, wherein the micro perfusion capillary has an inner diameter of 0.35-0.45 mm, an outer diameter of 0.50-0.60 mm, and a length of 18-23 cm; the size of the internal thread of the micro-perfusion capillary tube is 0.125mm in pitch, 0.500-0.600 mm in major diameter, 0.419-0.445 mm in middle diameter and 0.380-0.415 mm in minor diameter.

9. The direct implantation type open micro perfusion probe as claimed in claim 3, wherein the solid guiding needle has a diameter of 0.49mm and a length of 3-5 cm; the size of the external thread of the solid guide needle is 0.125mm in pitch, 0.480-0.500 mm in major diameter, 0.393-0.419 mm in intermediate diameter and 0.332-0.360 mm in minor diameter.

10. A direct implantation open micro perfusion probe according to claim 3, wherein the micro perfusion capillary has an inner diameter of 0.40mm and an outer diameter of 0.55 mm; the size of the internal thread of the micro-perfusion capillary is 0.125mm, the major diameter is 0.500mm, the middle diameter is 0.430mm, and the minor diameter is 0.400 mm; the external thread of the solid guide needle has the size of 0.125mm of thread pitch, 0.490mm of major diameter, 0.410mm of intermediate diameter and 0.360mm of minor diameter.