CN211407436U - Mechanical perfusion preservation device for organism organs - Google Patents
Mechanical perfusion preservation device for organism organs Download PDFInfo
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- CN211407436U CN211407436U CN201921442950.4U CN201921442950U CN211407436U CN 211407436 U CN211407436 U CN 211407436U CN 201921442950 U CN201921442950 U CN 201921442950U CN 211407436 U CN211407436 U CN 211407436U
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
- A01N1/0247—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components for perfusion, i.e. for circulating fluid through organs, blood vessels or other living parts
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Abstract
The utility model relates to a medical equipment provides a mechanical perfusion preservation device of organism organ, include: organ cartridge (2): for receiving organs and collateral vessels of a living body; liquid storage bin (3): the device is positioned below the organ bin (2) and used for storing circulating perfusate; temperature maintaining device (4): for maintaining the temperature of the organism organ and the circulating fluid; oxygenator (5): for continuously oxygenating the circulating perfusate; thrombus filter (6): for filtering the circulating perfusate; pump (7): pressurizing the circulating perfusion fluid; priming line (8): the liquid storage bin (3), the thrombus filter (6) and the oxygenator (5) are sequentially connected, and the organism organ is pressurized and circularly perfused under the driving of the pump (7); the mechanical perfusion preservation device can pressurize and circularly perfuse the aorta system of a living body, and can perfuse single or multiple organs of the living body simultaneously.
Description
Technical Field
The present invention relates to medical devices, and more particularly, to a mechanical perfusion preservation device for biological organs.
Background
With the rapid development of organ transplantation technology, the requirement for organ preservation technology is higher and higher, and the traditional UW liquid cryopreservation can not meet the clinical requirement, so that the novel mechanical perfusion preservation technology is gradually accepted by the organ transplantation center worldwide. The best mechanical perfusion preservation device should satisfy the following characteristics: 1) The device is portable, and is convenient to transport in the storage process; 2) synchronously performing real-time and dynamic evaluation on organ vitality during preservation; 3) repairing the damaged organ; 4) in the preservation process, medicines, gene editing or stem cell infusion and other treatments are synchronously carried out; 5) storing multiple organs simultaneously; 6) creating an in vitro environment closest to human physiology.
Current mechanical perfusion preservation techniques can only preserve a single Organ, for example Organ-OX and Liver-assist can only preserve the Liver alone, Kidney-assist and Life-Port can only preserve the Kidney alone, and Trans medicine can only preserve the heart alone. And when organ donation donor organ acquirement process, every donor need completely acquire 1 liver, 2 kidneys, 1 pancreas, 1 heart, 2 lungs, 1 small intestine simultaneously, 8 organs in total, then need a plurality of different organ preservation equipment, not only complicated, bulky but also difficult simultaneous operation, maintain simultaneously and transport, need to preserve these organs simultaneously or general type save set. Moreover, human organs supplement each other, and the simultaneous preservation of multiple organs is helpful for the mutual protection among organs.
In addition, in the medical training process, in order to make the training effects of surgical laparoscopes, natural orifice endoscopes, surgical robots, medical digestive endoscopes and the like more realistic, large animals need to be used for surgical operation training. However, with the progress of social civilization and the development of medical ethics, the factors of expensive cost for establishing a large animal operating room, expensive cost for a single large animal experiment and the like limit the operation training by using large animals. At present, medical training mostly uses edible large animal organs purchased in the market for training, but the organs are isolated dead organs without vitality and are single organs, so that the vitality is lacked, the difference between the survival state of the organs and the survival state of the body is large, bleeding cannot be accurately simulated, intestines cannot creep, livers cannot produce bile, kidneys cannot produce urine, and hearts cannot jump, so the simulation effect is poor; moreover, the whole anatomical structure of a single organ is destroyed, and the system anatomical training cannot be carried out, so that the training effect is poor. The virtual reality training devices appearing in recent years also have many defects, such as lack of anatomical variation, lack of authenticity of operation, high selling price and the like.
Chinese invention patent 201380046850.9 discloses an organ perfusion apparatus with downstream flow control, which mentions: a method of perfusing an organ or tissue, the method comprising: connecting a catheter to an organ or tissue, the catheter comprising a plurality of tubes, and a first end of each of at least two of the tubes being connected to the organ or tissue; applying a fluid motive force to the perfusion fluid in the two tubes to force the fluid through the two tubes into the organ or tissue to perfuse the organ or tissue; allowing a flow equilibrium to be established between the two tubes by the fluid motive force and the back pressure generated by the perfused organ or tissue; and then changing the flow balance between the two tubes without changing the applied fluid motive force. The invention patent is obviously different from the basic structure of the utility model, the invention patent is a multi-tube structure, does not comprise an oxygenator and a thrombus filter, and has different main parts.
Chinese patent application 201580002264.3 discloses a human body simulation device, comprising: an organ from an animal having a biological tissue and a blood vessel extending from the biological tissue; a blood reservoir portion for storing blood to be sent to the blood vessel; a tube connecting the blood vessel and the blood reservoir; a pump section provided in the middle of the tube and configured to transport blood from the blood reservoir section to the blood vessel; and a storage unit that stores the organ, humidifies the organ by supplying vapor having a temperature higher than room temperature to the organ, and sets the organ to a temperature of 20-50 ℃. The basic structure of the invention does not comprise an oxygenator and a thrombus filter, and a single organ is used, so that the simultaneous perfusion of multiple organs cannot be realized. Furthermore, this prior art technique achieves a unidirectional perfusion, whereas the organs of the body require a blood return circulation. Therefore, the organ mechanical perfusion device in the prior art is complex and heavy, and cannot realize multi-organ preservation, and multi-organ preservation cannot be realized.
SUMMERY OF THE UTILITY MODEL
In order to realize the clinical multi-organ isolated preservation, a multi-organ perfusion system taking the abdominal aorta or the thoracic aorta as the core must be established. In order to simplify the operation, the device of the utility model can simultaneously perfuse a plurality of organs or a single organ through the abdominal aorta or the thoracic aorta system through a single pump and a perfusion pipeline, wherein the portal vein of the liver does not need to be inserted with a tube, and the reflux perfusion is carried out by depending on the circulating perfusate through the intestinal artery system and the splenic artery; multiple thoracic organs can be perfused simultaneously through the thoracic aortic system, where the pulmonary arteries are perfused without cannulation with circulating perfusate from the right heart. In the case of preservation of multiple organs, only a single cannula is required, a significant simplification, which is a completely different basic principle than the prior art.
The utility model discloses a mechanical perfusion preservation device of organism organ, include:
organ storage: for receiving organs and collateral vessels of a living body;
a liquid storage bin: the organ bin is positioned below the organ bin and used for storing circulating perfusate;
temperature maintaining device: for maintaining the temperature of the organism organ and the circulating fluid;
an oxygenator: for continuously oxygenating the circulating perfusate;
a thrombus filter: for filtering the circulating perfusate;
a pump: pressurizing the circulating perfusion fluid;
filling a pipeline: the liquid storage bin, the thrombus filter and the oxygenator are sequentially connected, and the organism organ is pressurized and circularly perfused under the driving of the pump;
the mechanical perfusion preservation device can pressurize and circularly perfuse the aorta system of a living body, and can perfuse single or multiple organs of the living body simultaneously.
Further, the mechanical perfusion preservation device can perfuse a plurality of abdominal organs simultaneously through the abdominal aorta system, wherein the portal vein of the liver does not need to be cannulated, and perfusion is performed through the circulating perfusate returned by the portal vein system.
Further, the mechanical perfusion preservation device is capable of perfusing a plurality of thoracic organs simultaneously through the thoracic aortic system, wherein the pulmonary artery is perfused without cannulation with the circulating perfusate of the right heart.
Further, the perfusion preservation device further comprises: the blood vessel intubation is positioned at the tail end of the perfusion pipeline and is used for connecting the affiliated blood vessels of the organism organ for perfusion.
Further, the vascular cannula has a corresponding side hole with an inner diameter matching the position and inner diameter of the vascular cannula corresponding to the different branches within the aortic system.
Further, the temperature maintenance device is a water bath, and the organ bin and the liquid storage bin are placed in the water bath.
Further, the perfusion preservation device further comprises: and the oxygenator heating circulating pump is used for pumping out the warm water in the water bath box so as to circularly heat the oxygenator.
Further, the perfusion preservation device further comprises: the temperature maintaining device comprises a temperature controller which can control the temperature of the water bath tank.
Further, the perfusion preservation device further comprises: the temperature maintaining device is a compressor, ice blocks or an ultralow temperature agent and is used for maintaining the low temperature of the liquid storage bin, the organ bin and the organism organ.
Further, the perfusion preservation device further comprises: the temperature sensor is used for monitoring the temperature of the circulating perfusate and the organism organ in the liquid storage bin;
the flow sensor is used for monitoring the flow of the circulating perfusion liquid in the perfusion pipeline;
the pressure sensor is used for monitoring the pressure of the circulating perfusion liquid in the perfusion pipeline;
and the bubble sensor is used for monitoring whether bubbles exist in the filling pipeline or not.
Further, the perfusion preservation device further comprises: a controller that collects signals of the temperature sensor and controls the temperature maintenance device based on the signals.
Further, the perfusion preservation device further comprises: a controller that collects signals of the flow sensor and the pressure sensor and controls the pump based on the signals.
Further, the perfusion preservation device further comprises: a controller that collects signals of the flow sensor, the pressure sensor, and the bubble sensor, and controls the filling line clamp valve based on the signals, the filling line clamp valve being capable of stopping filling of the filling line.
Further, organ storehouse bottom has organ storehouse shelf, organ storehouse shelf have the through-hole with the stock solution storehouse communicates with each other.
Further, the perfusion preservation device further comprises: the device comprises a bile collecting and metering device for collecting bile and a urine collecting and metering device for collecting urine so as to dynamically observe the vitality of organism organs.
Compared with the prior art of the chinese patent application 201580002264.3, the utility model has the following differences: 1) the basic structure is different, the utility model discloses a device not only includes: organs and their connecting vessels, pump parts, tubes, heating devices, organ bins; also comprises an oxygenator, a thrombus filter and the like; 2) the pipeline structure of the device of the utility model is different from the Chinese patent application 201580002264.3, the utility model simultaneously fills abdominal cavity or thoracic cavity multiple organs through abdominal aorta or thoracic aorta system according to single pump and single blood vessel intubation, liver portal vein does not need intubation and depends on intestinal tract and spleen reflux perfusate for filling; the device can perfuse a plurality of thoracic organs simultaneously through a thoracic aorta system, wherein the pulmonary artery does not need to be cannulated and perfuses through the circulating perfusate of the right heart; 3) the utility model realizes blood reflux circulation, and Chinese patent application 201580002264.3 only adopts one-way perfusion, and does not mention circulation perfusion;
additionally, the beneficial effects of the utility model also include:
1) the device of the utility model is suitable for clinical organ preservation, restoration and vitality evaluation, is inserted into abdominal aorta or thoracic aorta and branches thereof through a single tube, and is used for simultaneously perfusing and preserving a plurality of organs, wherein the portal vein of the liver does not need to be inserted into a tube and depends on blood reflux perfusion perfused through an intestinal artery system and a spleen artery; wherein the pulmonary artery is perfused with blood from the right heart without cannulation. Can fully exert the mutual protection of multiple organs, such as the functions of discharging excessive water by the kidney, maintaining acid-base balance, liver metabolism function, immunity function and the like, the functions of absorbing water and nutrient substances by intestinal tracts, promoting liver repair and the like, and the functions of regulating blood sugar and promoting digestion by pancreas and the like. Multiple organs supplement each other and jointly reduce the risk of poor function or dysfunction of the graft after the organ transplantation.
2) The utility model discloses a device still is applicable to medical science minimal access training (peritoneoscope, natural cavity way scope, operation robot, digestion scope etc.), utilizes the simple and convenient structure of single intubate aorta system perfusion multiorgan, the discarded large animal organ that utilizes the pig farm to acquire clusters, it preserves to fill in vitro, utilize blood or cell-free type circulation perfusate, the circulation is filled and is supplemented the energy substrate and take oxygen, maintain the organ vigor, and do not destroy the anatomical structure of abdominal cavity or thorax organ, help the doctor to master conventional operation or minimal access technology basic operation. The method has the advantages of obviously reducing the cost, avoiding ethical problems, having anatomical variation, keeping an anatomical structure in vivo, keeping the vitality of organs, bleeding after injury and the like while approaching the effect of a large animal experiment to the maximum extent.
Drawings
Fig. 1 is a plan view schematically illustrating the basic structure of a first embodiment of the device of the present invention.
Fig. 2 is a perspective view of the basic structure of the first embodiment of the device of the present invention.
Fig. 3 is a schematic diagram of the basic principle of the first embodiment of the device of the present invention.
Fig. 4 is a top view of a second embodiment organ cartridge of the device of the present invention.
Fig. 5 is a top view of a core component of a second embodiment of the device of the present invention.
Fig. 6 is a side view of a second embodiment core component of the device of the present invention.
Fig. 7 is an exploded view of the core components of a second embodiment of the device of the present invention.
Fig. 8 is an overall appearance of a second embodiment oxygenator and filter plug assembly of the device of the present invention.
Fig. 9 is a cross-sectional view of a second embodiment oxygenator and plug assembly of the device of the present invention.
Fig. 10 is a schematic diagram of an electronic control system of a second embodiment of the apparatus of the present invention.
Fig. 11 is a schematic view of a bile and urine collection device according to a second embodiment of the device of the present invention.
Reference numerals
1-organism organ, 2-organ chamber, 3-liquid storage chamber, 4-temperature maintaining device, 5-oxygenator, 6-thrombus filtering device, 7-pump, 8-perfusion pipeline, 9-blood vessel intubation, 10-water bath tank, 14-diaphragm suspension, 16-bile collecting and metering device, 17-urine collecting and metering device, 22-diaphragm, 23-proximal abdominal aorta blocking ligature, 24-abdominal trunk, 25-splenic artery, 26-superior mesenteric artery, 27-distal abdominal aorta intubation ligature, 28-abdominal aorta, 29-superior and inferior hepatic vena cava, 30-liver, 31-intrinsic hepatic artery, 32-splenic vein, superior mesenteric vein junction with portal vein, 33-renal artery, 34-inferior hepatic vena cava, 42-temperature controller, 43-lifting column, 46-oxygen bottle, 54-oxygenator and thrombus filter shell, 58-organ bin shelf, 60-reflux pipeline, 63-oxygenator heating circulation interface, 64-blood vessel cannula connecting part, 65-oxygenator heater, 66-flow sensor, 67-controller, 68-temperature sensor, 69-pressure sensor, 70-perfusion pipeline clamping valve, 71-oxygenator heating circulation pump, 72-spleen, 73-stomach, 74-intestinal tract, 75-kidney, 76-portal vein, 77-splenic vein, 78-superior mesenteric vein, 79-inferior mesenteric vein, 80-right gastroretinal vein, 81-inferior mesenteric artery, 82-low-voltage direct current power supply, 83-control panel and display screen, 84-alarm device, 85-bubble sensor.
Detailed Description
Embodiments of the present invention will now be described with reference to the drawings, wherein like parts are designated by like reference numerals. The embodiments described below and the technical features of the embodiments may be combined with each other without conflict.
First embodiment
As shown in fig. 1 to 3, the device of the present invention is used for a living organism organ 1, the living organism organ 1 includes a living organism organ and a blood vessel attached from the living organism organ, and the living organism organ 1 may be an organ cluster or a single organ.
The device of the utility model is used for the mechanical perfusion to preserve organism organ 1, include: organ chamber 2, stock solution storehouse 3, temperature maintenance device 4, oxygenator 5, filter 6, pump 7 and perfusion circuit 8. Preferably, a vascular cannula 9 is included at the end of the perfusion circuit 8.
The organ container 2 is used for accommodating a living organ 1 and blood vessels attached from the living organ; the liquid storage bin 3 is positioned at the lower part of the organ bin 2 and is used for collecting circulating perfusate which flows back from the vein end of the organism organ; the temperature maintaining device 4 is used for maintaining the temperature of the organism organ 1, the organ bin 2, the liquid storage bin 3 and the whole device; the oxygenator 5 is used for oxygenating the circulating perfusate, and the oxygenator 5 is connected with an oxygen bottle 46; the thrombus filter 6 is used for filtering thrombus, bubbles and other impurities in the circulating perfusate; the pump 7 is used for maintaining the perfusion power of the device, pressurizing perfusion and circulating perfusion; the filling pipeline 8 is used for connecting each key component; the vessel cannula 9 is positioned at the end of the perfusion pipeline 8 and is connected with the aorta system of the organism organ 1 for pressurized perfusion and circulating perfusion. Preferably, the vascular cannula 9 has corresponding side holes in the aortic system at corresponding positions of the different branches, with an internal diameter matching it, allowing a more accurate perfusion of the corresponding organs. The vessel cannula 9 may also have multiple branches.
The portal vein 76 is perfused without cannulation, relying on the return perfusate from the splenic artery 25 to the spleen 72, and the return perfusate from the superior mesenteric artery 26 to the intestinal tract 74, after the splenic vein, superior mesenteric vein and portal vein junction 32 have merged.
As shown in fig. 2, the basic working principle of the device is as follows: the biological organ 1 is placed in the organ chamber 2, the temperature is maintained by the temperature maintaining device 4 outside the organ chamber 2 (the organ chamber 2 is placed in the temperature maintaining device 4), the circulating perfusate (including blood or non-cell type circulating fluid) flows back and is stored in the liquid storage chamber 3, and the liquid storage chamber 3 is positioned below the organ chamber 2. The organ chamber shelf 58 of the organ chamber 2 is provided with through holes, so that the organ chamber 2 and the liquid storage chamber 3 are physically communicated, and perfusate conveniently flows back to the liquid storage chamber 3 at the lower layer of the organ chamber after flowing out of the biological organ 1. In one embodiment (as shown in fig. 6 and 7), the temperature maintaining device 4 is in the form of a water bath 10, and not only can directly heat the organ chamber 2 immersed in the water bath 10, but also an oxygenator heating circulating pump 71 (disposed inside or outside the water bath 10) can pump out hot water in the water bath 10 to circularly heat the oxygenator 5, thereby improving heating efficiency. In another embodiment, the temperature maintaining device 4 is in the form of a compressor, ice, or ultra-low temperature agent, and maintains the low temperature of the liquid storage 3, the organ storage 2, and the living body organ 1 therein.
Under the drive of the pump 7, the circulating perfusion fluid in the liquid storage bin 3 enters the thrombus filter 6 to filter impurities and bubbles in the perfusion fluid; then oxygenated by an oxygenator 5; then enters the aorta system of the organism organ 1 in the organ chamber 2 through the circulation pipeline 8 and the blood vessel intubation tube 9, and then perfuses a plurality of organs simultaneously by utilizing different branches of the aorta; finally, circulating perfusate and the like flow out through the vein end of the organism organ 1, are collected and refluxed and concentrated in the liquid storage bin 3, and thus form circulating perfusion in a reciprocating mode.
The circulation sequence is sequentially organism organ 1 in the organ chamber 2, liquid storage chamber 3, embolism filtering device 6, pump 7, oxygenator 5, perfusion pipeline 8 (blood vessel intubation tube 9) and organism organ 1 in the organ chamber 2. The filling line 8 connects the main components.
The utility model discloses an organ preservation device perfuses a plurality of organs simultaneously through abdominal aorta or thoracic aorta system, and wherein the portal vein 76 of liver need not the intubate, and the circulation perfusate of through portal vein system backward flow perfuses. Wherein the pulmonary artery is perfused by the circulating perfusate of the right heart without cannulation.
Optionally, a vessel cannula 9 is positioned at the end of the perfusion circuit 8 to connect to a collateral vessel of the biological organ 1 for perfusion.
Optionally, the vessel cannula 9 has corresponding side holes at corresponding positions of different branches in the aortic system, and the inner diameter is matched with the side holes, so that the corresponding organs can be perfused more accurately.
As shown in FIG. 3, in using the present device, first, a living organ 1 and its collateral vessels are placed in an organ chamber 2, and all organs are arranged in a normal anatomical configuration. Then, both ends of the abdominal aorta 28 are respectively ligated by the proximal abdominal aorta occlusion ligature 23 and the distal abdominal aorta occlusion ligature 27, and the circulation line 8 (optionally, the distal end connected to the blood vessel cannula 9) is inserted into the abdominal aorta 28 and fixed by the ligatures. The circulating perfusate is pressurized by the pump 7, injected into the abdominal aorta 28 through the circulating line 8 (optionally, connected at the end to the vessel cannula 9), and then perfused through the organs through the abdominal aorta branches. Wherein bilateral kidneys 75 are perfused through renal artery 33; arterial perfusion of the liver 30 is performed through the celiac trunk 24 branching hepatic artery 31; and spleen 72 is perfused through celiac trunk 24 branch splenic artery 25; arterial infusion of the intestinal tract 74 through the superior mesenteric artery 26, the inferior mesenteric artery 81; the vein of the intestinal tract 74 returns to the superior mesenteric vein 78 and the inferior mesenteric vein 79, the vein of the spleen 72 returns to the splenic vein 77, and the vein of the stomach 73 returns to the right gastroomentum vein 80, and the splenic vein, the superior mesenteric vein and the portal vein junction 32 are converged together, and the liver is perfused through the portal vein 76, so the portal vein 76 can realize perfusion without a cannula. The device can complete perfusion of the abdominal cavity viscera only by perfusing the abdominal aorta 28 and the branches thereof through a single pipe. All the circulating perfusate flows back to the organ bin 2 and the liquid storage bin 3 through the suprahepatic vena cava 29 and the infrahepatic vena cava 34 of the inferior vena cava system, and then the circulating perfusate in the liquid storage bin 3 enters the thrombus filtering device 6 under the driving of the pump 7 to filter impurities and bubbles in the circulating perfusate; then oxygenated by an oxygenator 5; then enters the aorta system of the organism organ 1 in the organ chamber 2 through a circulating pipeline 8 (optionally, the tail end is connected with a blood vessel cannula 9), and then a plurality of organs are perfused simultaneously by using different branches of the aorta, and the circulation is repeated;
the device can simultaneously perfuse a plurality of thoracic organs through a thoracic aortic system, wherein the pulmonary artery is perfused through the circulating perfusate of the right heart without intubation.
Optionally, a vessel cannula 9 is positioned at the end of the perfusion circuit 8 to connect to a collateral vessel of the biological organ 1 for perfusion.
Optionally, the vessel cannula 9 has corresponding side holes at corresponding positions of different branches in the aortic system, and the inner diameter is matched with the side holes, so that the corresponding organs can be perfused more accurately.
Optionally, the utility model discloses a device can be used to external machinery and pours into human organ of preserving for many organs are preserved, play its key mutual guard action, preserve and restore simultaneously, aassessment organ vigor.
Optionally, the utility model discloses an animal organs such as pig or sheep are used to the device, and external machinery is perfused, resumes organ vigor, and human organ is simulated to the at utmost, carries out medical training.
Second embodiment
A second embodiment of the present invention will be described with reference to fig. 4-10, further detailing the structure and principles of the components of the organ chamber 2, reservoir 3, temperature maintenance device 4, oxygenator 5 and embolectomy filter 6.
As shown in fig. 4, the bottom of the organ chamber 2 is provided with an organ chamber shelf 58, which is provided with a plurality of holes and is communicated with the lower liquid storage chamber 3 (see fig. 2) to facilitate the perfusion circulation liquid to flow back to the liquid storage chamber 3. The inner side of the organ chamber 2 is provided with a return pipeline 60, one end of the return pipeline 60 is communicated with the liquid storage chamber 3, and the other end is connected with the circulating pipeline 8. Preferably, the bottom surface of the reservoir 3 is inclined toward the connection of the return line 60, so as to maintain a high liquid level, thereby facilitating collection of the circulating liquid and preventing the return line 60 from sucking air. The outer wall of the organ chamber 2 is provided with an oxygenator heating circulation interface 63 for pumping warm water in the liquid storage chamber 3 below the circulation organ chamber 2 and heating the circulation perfusate through the oxygenator 5. The oxygenator heating cycle port 63 is not in communication with the organ chamber 2 and is in communication with the water bath 10 (described in detail below).
The side wall of the organ cabin 2 is provided with a diaphragm suspension 14 for suspending a diaphragm 22 (see fig. 1), the diaphragm suspension 14 is a protrusion protruding inwards from the inner wall of the organ cabin 2, and a plurality of diaphragm suspensions 14 can be arranged. The diaphragm hook 14 is used for fixing and hanging the diaphragm part of the organism organ 1, and restoring the anatomical position of the liver in the abdominal cavity to the maximum extent.
As shown in fig. 5-7, the device of the present invention comprises: organ chamber 2, water bath 10 (optional as temperature maintenance device 4), pump 7, oxygenator 5, embolectomy filter 6, return line 60, vessel cannula connection 64, and vessel cannula 9. Wherein, the return end of the perfusion pipeline 8 is connected with the return pipeline 60, the perfusion end is connected with the blood vessel intubation connecting part 64 and the blood vessel intubation 9, and the middle of the perfusion pipeline 8 is connected with the pump 7, the oxygenator 5, the thrombus filter 6 and other parts. The organism organ 1 is placed on an organ chamber shelf 58 in the organ chamber 2, and is immersed in the water bath box 10 together to maintain the body temperature environment, and the diaphragm hook 14 is used for fixing and hanging the diaphragm part of the organism organ 1, thereby reducing the anatomical position in the abdominal cavity to the maximum extent. Alternatively, the organ shelf 58 of the organ bin 2 may be made of metal as a negative plate of the high-frequency electric knife 15 in order to use the high-frequency electric knife 15.
The circulating perfusate enters the abdominal aorta or thoracic aorta system of the organism organ 1 through the blood vessel cannula 9 under the pressurization driving of the pump 7, and perfuses a plurality of organs in the abdominal cavity or the thoracic cavity simultaneously, the portal vein 76 does not need to be cannulated, and depends on the reflux perfusate from the spleen artery 25 to the spleen 72, the superior mesenteric artery 26 perfusate the reflux perfusate of the intestinal tract 74, and the perfusion is performed after the spleen vein and the superior mesenteric vein are merged with the portal vein junction 32. The pulmonary artery is perfused with the circulating perfusate from the right heart without cannulation. Then the circulating perfusate flows out through the inferior vena cava of the organism organ 1 and intensively flows back to the liquid storage bin 3 at the lower part of the organ bin 2 through the porous structure of the organ bin shelf 58; then, under the drive of the pump 7, the circulating liquid in the liquid storage bin 3 is pumped out through the return pipeline 60 and flows to the thrombus filter 6, and thrombus and air embolism mixed in the circulating liquid are filtered; then passes through a pump 7 through a perfusion pipeline 8 through an outlet of the filter suppository device 6, and then enters the oxygenator 5 through the perfusion pipeline 8 to oxygenate the circulating liquid; then enters the blood vessel intubation connecting part 64 from the outlet of the oxygenator 5, and enters the blood vessel intubation 9 through the blood vessel intubation connecting part 64, thus completing the circulation perfusion process.
As shown in fig. 6 to 9, the organ chamber 2 and the reservoir chamber 3 are placed in the temperature maintenance apparatus 4, and the temperature maintenance apparatus 4 includes a water bath 10 and a temperature controller 42. The temperature is maintained through two ways, namely, the first way, warm water is contained in the water bath box 10 to directly heat the organ bin 2, the liquid storage bin 3 and the organism organ 1. In the second approach, the oxygenator heating circulation pump 71 is arranged to pump the warm water in the water bath 10 through the oxygenator heating circulation port 63 into the oxygenator heater 65, and the oxygenator heater 65 is located below the oxygenator 5 to heat the circulation liquid in the oxygenator 5.
The oxygenator 5 and the thrombus filter 6 are intensively arranged in the oxygenator and thrombus filter shell 54, and the corresponding circulating pipelines 8 are regularly distributed in the shell 54, so that the oxygenator and thrombus filter is convenient to install and replace quickly; optionally, the components of the organ container 2, the reservoir 3, the oxygenator 5, the embolus filter 6, the perfusion line 8, the blood vessel cannula 9, the return line 60, etc. other than the pump 7 can be disposable, facilitating quick replacement.
Referring again to fig. 6, a temperature sensor 68 is disposed inside the organ cartridge 2 for monitoring the temperature of the living organism organ 1 inside the organ cartridge 2. Flow sensors 66 are disposed on the return line 60 and the vessel cannula connection 64 for monitoring the flow of the perfusion fluid circulating through the return line 60 and the vessel cannula connection 64. The pressure sensor 69 is arranged on the blood vessel intubation connecting part 64, wherein the blood vessel intubation 9 is connected with the tail end of the blood vessel intubation connecting part 64. The pressure sensor 69 is used to monitor the pressure at the vascular cannula connection 64, i.e. the pressure of the circulating perfusion fluid to be delivered to the vascular cannula 9. The bubble sensor 85 is used to monitor bubbles in the irrigation line 8.
Referring again to fig. 6, the organ perfusion preservation device of the present invention further includes a perfusion line clamp valve 70, which is disposed on the perfusion line 8, and is shown as being disposed near the input end of the pump 7, the perfusion line clamp valve 70 closes the circulation perfusion that can stop the perfusion line 8 quickly, and the organism organ 1 is prevented from being damaged when abnormal conditions occur.
As shown in fig. 10, the organ perfusion preservation apparatus of the present invention further includes an electronic control system, which is powered by a low voltage dc power supply 82. The electronic control system includes a controller 67 and a control panel and display screen 83. Fig. 10 shows a schematic circuit diagram of the electronic control system. Wherein, the utility model discloses a device can be furnished with lift post 43, and lift post 43 is installed in stock solution storehouse 3 below, can the height in stock solution storehouse 3 (together with organ storehouse 2) of lifting. The utility model discloses a device still includes temperature sensor 68, flow sensor 66, pressure sensor 69 and bubble sensor 85, the signal of above-mentioned sensor spreads into controller 67 analysis processes back into, through controller 67 control temperature maintenance device 4's temperature, the rotational speed of control pump 7 is unusual in order to prevent to fill pressure or flow, the control is filled the pipeline and is clamped the valve 70 state of opening and shutting, carry out timely alarm through alarm device 84 when the device takes place the abnormal conditions, the alarm can show on control panel and display screen 83.
As shown in fig. 11, the system of the present invention further has a bile collecting and metering device 16 and a urine collecting and metering device 17 for collecting bile and urine and dynamically observing the vitality of organs.
The above-mentioned embodiments are only preferred embodiments of the present invention, and the ordinary changes and substitutions performed by those skilled in the art within the technical scope of the present invention should be included in the protection scope of the present invention.
Claims (15)
1. A device for mechanical perfusion preservation of an organ of a living being, comprising:
organ cartridge (2): for receiving organs and collateral vessels of a living body;
liquid storage bin (3): the device is positioned below the organ bin (2) and used for storing circulating perfusate;
temperature maintaining device (4): for maintaining the temperature of the organism organ and the circulating fluid;
oxygenator (5): for continuously oxygenating the circulating perfusate;
thrombus filter (6): for filtering the circulating perfusate;
pump (7): pressurizing the circulating perfusion fluid;
priming line (8): the liquid storage bin (3), the thrombus filter (6) and the oxygenator (5) are sequentially connected, and the organism organ is pressurized and circularly perfused under the driving of the pump (7);
the mechanical perfusion preservation device can pressurize and circularly perfuse the aorta system of a living body, and can perfuse single or multiple organs of the living body simultaneously.
2. The perfusion preservation device of claim 1,
the mechanical perfusion preservation device is capable of simultaneously perfusing a plurality of abdominal organs through the abdominal aortic system, wherein the portal vein (76) of the liver is perfused without cannulation by a circulating perfusate that is returned by the portal vein system.
3. The perfusion preservation device of claim 1,
the mechanical perfusion preservation device can simultaneously perfuse a plurality of thoracic organs through a thoracic aortic system, wherein the pulmonary artery is perfused without cannulation by a circulating perfusate of the right heart.
4. The perfusion preservation device of claim 1, further comprising:
the blood vessel intubation tube (9), the blood vessel intubation tube (9) is positioned at the tail end of the perfusion pipeline (8) and is used for connecting the subsidiary blood vessel of the organism organ (1) for perfusion.
5. The perfusion preservation device of claim 4,
the vascular cannula (9) has respective side holes with an internal diameter matching the position and internal diameter of the vascular cannula (9) corresponding to the different branches of the aortic system.
6. The perfusion preservation device of claim 1,
the temperature maintaining device (4) is a water bath box (10), and the organ bin (2) and the liquid storage bin (3) are placed in the water bath box (10).
7. The perfusion preservation device of claim 6, further comprising:
and an oxygenator heating circulation pump (71) for circulating and heating the oxygenator (5) by pumping out the warm water in the water bath (10).
8. The perfusion preservation device of claim 6, further comprising:
the temperature maintenance device (4) comprises a temperature controller (42), the temperature controller (42) being capable of controlling the temperature of the water bath tank (10).
9. The perfusion preservation device of claim 1, further comprising:
the temperature maintaining device (4) is a compressor, ice blocks or an ultralow temperature agent and is used for maintaining the low temperature of the liquid storage bin (3), the organ bin (2) and the organism organ.
10. The perfusion preservation device of claim 6, further comprising:
the temperature sensor (68) is used for monitoring the temperature of the circulating perfusate and the organism organ in the liquid storage bin (3);
a flow sensor (66) for monitoring the flow of the circulating perfusion fluid in the perfusion line (8);
a pressure sensor (69) for monitoring the pressure of the circulating perfusion fluid in the perfusion line (8);
and the air bubble sensor (85) is used for monitoring whether air bubbles exist in the perfusion pipeline (8).
11. The perfusion preservation device of claim 10, further comprising:
a controller (67) that collects signals of a temperature sensor (68) and controls the temperature maintenance device (4) based on the signals.
12. The perfusion preservation device of claim 10, further comprising:
a controller (67) that collects signals of a flow sensor (66) and a pressure sensor (69) and controls the pump (7) based on the signals.
13. The perfusion preservation device of claim 10, further comprising:
a controller (67) that collects signals of the flow sensor (66), the pressure sensor (69), and the bubble sensor (85), and controls a perfusion line clamp valve (70) based on the signals, the perfusion line clamp valve (70) being capable of stopping perfusion of the perfusion line (8).
14. The perfusion preservation device of claim 1,
organ storehouse shelf (58) have in organ storehouse (2) bottom, organ storehouse shelf (58) have the through-hole with stock solution storehouse (3) communicate with each other.
15. The perfusion preservation device of claim 1, further comprising:
a bile collecting and metering device (16) for collecting bile and a urine collecting and metering device (17) for collecting urine so as to dynamically observe the vitality of the organism organ (1).
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| CN201921442950.4U Active CN211407436U (en) | 2019-03-22 | 2019-09-02 | Mechanical perfusion preservation device for organism organs |
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| CN110432260A (en) * | 2019-03-22 | 2019-11-12 | 天一阁医疗科技(广州)有限公司 | A kind of machine perfusion save set of organism organ |
| CN110459113A (en) * | 2019-03-22 | 2019-11-15 | 天一阁医疗科技(广州)有限公司 | A kind of organ perfusion's teaching and training system |
| US20230069897A1 (en) * | 2020-02-12 | 2023-03-09 | University Of Newcastle Upon Tyne | Apparatus for storing an organ or body tissue, and corresponding method |
| EP3977854A1 (en) * | 2020-10-02 | 2022-04-06 | Aferetica S.r.l. | Thermoregulation system for an ex vivo organ perfusion system and corresponding ex vivo organ perfusion system |
| CN113016784A (en) * | 2021-03-22 | 2021-06-25 | 上海长征医院 | Isolated limb machinery fills preservation case |
| CN113142192B (en) * | 2021-05-19 | 2024-05-17 | 北京盖兰德生物科技有限公司 | Isolated liver perfusion activity maintaining device and use method thereof |
| CN113303325A (en) * | 2021-05-27 | 2021-08-27 | 南方医科大学南方医院 | Perfusate and normal-temperature blood mechanical perfusion system suitable for rat donor heart |
| CN113412834B (en) * | 2021-08-25 | 2021-11-09 | 中山大学附属第一医院 | Multi-organ repairing and transferring device without interrupting blood flow |
| FR3126283B1 (en) | 2021-08-30 | 2024-06-21 | Stella Surgical | DEVICE AND METHOD FOR GRAFT MONITORING |
| CN113892483A (en) * | 2021-09-07 | 2022-01-07 | 奇点医疗科技(广州)有限公司 | Anti-layering filling structure and filling device |
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| CN115885972B (en) * | 2022-10-24 | 2024-09-03 | 中国科学院理化技术研究所 | Organ perfusion preservation apparatus |
| CN117898283A (en) * | 2023-12-28 | 2024-04-19 | 奇点医疗科技(广州)有限公司 | Device for keeping heart beat free donor organ activity |
| CN117547672B (en) * | 2024-01-02 | 2024-04-05 | 中山大学附属第一医院 | DCD-based in-vitro abdominal viscera perfusion and transplantation system |
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| WO2020192513A1 (en) | 2020-10-01 |
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