WO2020192513A1

WO2020192513A1 - Mechanical perfusion preservation device for living body's organs

Info

Publication number: WO2020192513A1
Application number: PCT/CN2020/079874
Authority: WO
Inventors: 何椰
Original assignee: 天一阁医疗科技(广州)有限公司
Priority date: 2019-03-22
Filing date: 2020-03-18
Publication date: 2020-10-01
Also published as: CN110432260A; CN211407436U

Abstract

The present invention relates to a piece of medical equipment. Provided is a mechanical perfusion preservation device for a living body's organ. The device comprises: an organ bin (2) for accommodating the living body's organ and accessory vessels thereof; a liquid storage bin (3) located below the organ bin (2) and used for storing circulation perfusate; a temperature maintaining device (4) for maintaining the temperatures of the living body's organ and the circulation perfusate; an oxygenator (5) for continuously oxygenating the circulation perfusate; a thrombus filter (6) for filtering the circulation perfusate; a pump (7) for pressurizing the circulation perfusate; and a perfusion pipeline (8) for sequentially connecting the liquid storage bin (3), the thrombus filter (6) and the oxygenator (5) and pressurizing and circularly perfusing the living body's organ under the drive of the pump (7). The mechanical perfusion preservation device may pressurize and circularly perfuse an aorta system of a living body and may perfuse one or more living body organs simultaneously. On the basis of the principles for the mechanical perfusion of organs, the device of the present invention may simultaneously perfuse a plurality of organs by using oxygenated blood or a cell component-free circulation perfusate.

Description

Mechanical perfusion preservation device for biological organs

Technical field

The present invention relates to a medical device, and more specifically, to a mechanical perfusion preservation device for biological organs.

Background technique

With the rapid development of organ transplantation technology, the requirements for organ preservation technology are getting higher and higher. The traditional UW liquid cryopreservation can no longer meet the clinical needs, so the new mechanical perfusion preservation technology has gradually been accepted by organ transplant centers worldwide. . The best mechanical perfusion preservation device should meet the following characteristics: 1) The device is portable and easy to transport during the preservation process; 2) Real-time and dynamic assessment of organ vitality is performed simultaneously during preservation; 3) The damaged organ is repaired; 4) During the preservation process, treatments such as drugs, gene editing or stem cell infusion are carried out simultaneously; 5) simultaneous preservation of multiple organs; 6) creation of an in vitro environment closest to human physiology.

The current mechanical perfusion preservation technology can only save a single organ. For example, Organ-OX and Liver-assist can only save the liver, Kidney-assist and Life-Port can only save the kidney, and TransMedics can only save the heart. In the process of organ donation from a donor organ, each donor needs to obtain 1 liver, 2 kidneys, 1 pancreas, 1 heart, 2 lungs, and 1 small intestine at the same time, a total of 8 organs. Many different organ preservation devices are not only complicated and large, but also difficult to operate, maintain, and transport at the same time. Therefore, a universal preservation device that can store these organs at the same time is required. Moreover, human organs complement each other, and the simultaneous preservation of multiple organs helps to play the role of mutual protection between organs.

In addition, in the process of medical training, in order to make the training effect of surgical laparoscopy, natural cavity endoscopy, surgical robot, and internal digestive endoscopy more realistic, it is necessary to use large animals for surgical operation training. However, with the progress of social civilization and the development of medical ethics, coupled with the high cost of establishing a large animal operating room, and the high cost of a single large animal experiment, all factors have restricted the use of large animals for surgical training. At present, most medical training uses the large edible animal organs bought on the market for training. However, these organs are often dead organs in vitro without vitality, and are single organs. Not only are they lacking vitality, but the organs are different from in vivo survival. It is too large and cannot accurately simulate bleeding, the intestines cannot move, the liver cannot produce bile, the kidneys cannot produce urine, and the heart cannot beat, so the simulation effect is poor; and the overall anatomy of a single organ has been destroyed, and systematic anatomy training and training cannot be performed. The effect is poor. Virtual reality training devices that have emerged in recent years also have many defects, such as lack of anatomical variation, lack of authenticity in surgical operations, and high prices.

Chinese invention patent 201380046850.9 discloses an organ perfusion device with downstream flow control. Claim 1 of the invention mentions: a method for perfusing an organ or tissue, the method comprising: connecting a catheter to the organ or tissue, the catheter including multiple Root canal, and at least the first end of each of the two tubes is connected to the organ or tissue; fluid motive force is applied to the perfusion fluid in the two tubes to force the fluid through the The two tubes enter the organ or tissue to perfuse the organ or tissue; allowing the fluid motive force and the back pressure generated by the perfused organ or tissue to establish a flow balance between the two tubes; And then change the flow balance between the two tubes without changing the fluid motive force applied. This invention patent is obviously different from the basic structure of the present invention. The invention patent is a multi-tube structure, excluding the oxygenator and the plug filter, and the main components are different.

Chinese invention patent application 201580002264.3 discloses a device that simulates the human body, including: an animal-derived organ, which has biological tissue and blood vessels extending from the biological tissue; and a blood reservoir, which stores blood delivered to the blood vessel A tube that connects the blood vessel and the blood reservoir; a pump portion that is provided in the middle of the tube and transports blood from the blood reservoir to the blood vessel; and a storage portion that accommodates the organ, The steam at a temperature higher than room temperature is delivered to the organ to humidify the organ, and the organ is set to a temperature of 20-50°C. The basic structure of the invention does not include an oxygenator and a thrombus filter, and uses a single organ, which cannot achieve simultaneous perfusion of multiple organs. Moreover, the prior art realizes unidirectional perfusion, and human organs need blood to return and circulate. It can be seen that the prior art mechanical perfusion device for organs is complex, heavy, and does not realize multi-organ preservation, nor can it realize multi-organ preservation.

Summary of the invention

In order to achieve clinical preservation of multiple organs simultaneously in vitro, a multi-organ perfusion system with abdominal aorta or thoracic aorta as the core must be established. In order to simplify the operation, the device of the present invention uses a single pump and a perfusion line to simultaneously perfuse multiple organs or a single organ through the abdominal aorta or thoracic aorta system. The liver and portal vein do not need to be intubated, and rely on the intestinal artery system and splenic artery. The circulatory perfusate is used for reflux perfusion; multiple thoracic organs can be perfused at the same time through the thoracic aortic system, in which the pulmonary artery does not need to be intubated, and the circulatory perfusate of the right heart is used for perfusion. In the case of preserving multiple organs, only a single cannula is required, which is significantly simplified, which is a fundamental principle completely different from the prior art.

The invention discloses a mechanical perfusion preservation device for biological organs, including:

Organ warehouse: used to contain the organs and accessory blood vessels of the organism;

Liquid storage bin: located below the organ bin for storing circulating perfusion fluid;

Temperature maintenance device: used to maintain the temperature of biological organs and circulating fluid;

Oxygenator: used to continuously oxygenate the circulating perfusate;

Plug filter: used to filter circulating perfusate;

Pump: pressurize the circulating perfusate;

Perfusion pipeline: connect the reservoir, the thrombus filter and the oxygenator in sequence, and pressurize and circulate the organs under the drive of the pump;

The mechanical perfusion preservation device can pressurize and circulate the aortic system of an organism, and can perfuse a single or multiple organism organs at the same time.

Further, the mechanical perfusion preservation device can simultaneously perfuse multiple abdominal organs through the abdominal aortic system, wherein the liver portal vein does not need to be cannulated, and the perfusion is performed through the circulating perfusate returned from the portal vein system.

Further, the mechanical perfusion preservation device can simultaneously perfuse multiple thoracic organs through the thoracic aortic system, wherein the pulmonary artery does not need to be intubated and is perfused with the circulating perfusion fluid of the right heart.

Further, the perfusion preservation device further includes: a blood vessel cannula, the blood vessel cannula is located at the end of the perfusion pipeline, and is used to connect the accessory blood vessels of the biological organs for perfusion.

Further, the blood vessel cannula has a corresponding side hole, and the inner diameter of the side hole matches the corresponding positions and inner diameters of the different branches of the blood vessel cannula in the aortic system.

Further, the temperature maintaining device is a water bath box, and the organ bin and the liquid storage bin are placed in the water bath box.

Further, the perfusion preservation device further includes: an oxygenator heating circulation pump, which is used to pump out warm water from the water bath to heat the oxygenator in circulation.

Further, the perfusion preservation device further includes: the temperature maintaining device includes a temperature controller, and the temperature controller can control the temperature of the water bath.

Further, the perfusion preservation device further includes: the temperature maintaining device is a compressor, ice cubes or ultra-low temperature agent, which is used to maintain the low temperature of the liquid storage tank, the organ tank, and the biological organs.

Further, the perfusion preservation device further includes a temperature sensor for monitoring the temperature of the circulating perfusion fluid and the biological organs in the liquid storage tank;

Flow sensor, used to monitor the flow of circulating perfusion fluid in the perfusion pipeline;

Pressure sensor, used to monitor the pressure of the circulating perfusion fluid in the perfusion pipeline;

The bubble sensor is used to monitor whether there are bubbles in the perfusion pipeline.

Further, the perfusion preservation device further includes a controller, which collects the signal of the temperature sensor and controls the temperature maintenance device based on the signal.

Further, the perfusion preservation device further includes a controller, which collects signals from the flow sensor and the pressure sensor, and controls the pump based on the signals.

Further, the perfusion preservation device further includes: a controller that collects the signals of the flow sensor, the pressure sensor and the air bubble sensor, and controls the perfusion pipeline clamp valve based on the signals, and the perfusion pipeline clamp valve can stop the perfusion pipe Road perfusion.

Further, the bottom of the organ bin has an organ bin shelf, and the organ bin shelf has a through hole that communicates with the liquid storage bin.

Further, the perfusion preservation device further includes: a bile collecting and measuring device for collecting bile and a urine collecting and measuring device for collecting urine, so as to dynamically observe the vitality of biological organs.

Compared with the prior art of Chinese patent application 201580002264.3, the present invention has the following differences: 1) The basic structure is different. The device of the present invention not only includes: organs and their connecting blood vessels, pumps, tubes, heating devices, organ bins ; It also includes oxygenators, thrombus filters, etc.; 2) The pipeline structure of the device of the present invention is different from Chinese patent application 201580002264.3. The present invention is based on a single pump and a single vessel cannula through the abdominal aorta or thoracic aortic system , Perfuse multiple organs in the abdominal or thoracic cavity at the same time. The portal vein of the liver does not need to be intubated and relies on the intestinal and splenic return perfusate for perfusion; this device can simultaneously perfuse multiple thoracic organs through the thoracic aortic system. The circulatory perfusion solution of the heart is perfused; 3) The present invention realizes the blood return circulation, and the Chinese patent application 201580002264.3 is only one-way perfusion without mentioning circulatory perfusion;

In addition, the beneficial effects of the present invention also include:

1) The device of the present invention is suitable for clinical organ preservation, repair and vitality assessment. A single tube is inserted into the abdominal aorta or thoracic aorta and its branches, and multiple organs are perfused at the same time. Among them, the liver portal vein does not need to be intubated and depends on passing through the intestine The arterial system and the splenic artery are perfused by the backflow of blood; the pulmonary artery does not need to be cannulated and is perfused through the right heart. Can give full play to the mutual protection of multiple organs, such as the kidneys excrete excess water and maintain acid-base balance, liver metabolism and immune function, etc., the intestine absorbs water and nutrients and promotes liver repair functions, and the pancreas regulates blood sugar and promotes digestion. Features. Multiple organs complement each other and jointly reduce the risk of graft dysfunction or loss of function after organ transplantation.

2) The device of the present invention is also suitable for medical minimally invasive training (laparoscopy, natural cavity endoscopy, surgical robots, digestive endoscopy, etc.), using a simple structure of single-intubation aortic system to infuse multiple organs, and using pig farms Obtained discarded large animal organ clusters are perfused and preserved in vitro, using blood or cell-free circulating perfusion fluid to circulate perfusion to supplement energy substrates and carry oxygen to maintain organ vitality without destroying the anatomical structure of abdominal or thoracic organs, helping doctors Master the basic operations of conventional surgery or minimally invasive techniques. While it is close to the effect of large animal experiments to the greatest extent, it has obvious advantages such as cost reduction, ethical problems avoided, anatomical variation, maintenance of the anatomical structure in the body, vitality of organs, and bleeding after injury.

Description of the drawings

Fig. 1 is a schematic plan view of the basic structure of the first embodiment of the device of the present invention.

Fig. 2 is a three-dimensional schematic diagram of the basic structure of the first embodiment of the device of the present invention.

Fig. 3 is a schematic diagram of the basic principle of the first embodiment of the device of the present invention.

Figure 4 is a top view of the organ compartment of the second embodiment of the device of the present invention.

Fig. 5 is a top view of the core components of the second embodiment of the device of the present invention.

Fig. 6 is a side view of the core components of the second embodiment of the device of the present invention.

Figure 7 is an exploded view of the core components of the second embodiment of the device of the present invention.

Figure 8 is the overall appearance of the oxygenator and plug filter assembly of the second embodiment of the device of the present invention.

Figure 9 is a cross-sectional view of the oxygenator and plug filter assembly of the second embodiment of the device of the present invention.

Fig. 10 is a schematic diagram of the electronic control system of the second embodiment of the device of the present invention.

Fig. 11 is a schematic diagram of a bile and urine collection device of the second embodiment of the device of the present invention.

Reference signs:

1-Organism, 2-Organ storage, 3-Reservoir, 4-Temperature maintenance device, 5-Oxygenator, 6-Thrombus filter, 7-Pump, 8-Perfusion line, 9-Vascular cannula , 10-water bath, 14-diaphragm suspension, 16-bile collection and measurement device, 17-urine collection and measurement device, 22-diaphragm, 23-proximal abdominal aorta blocking ligature, 24-abdominal trunk, 25-spleen Artery, 26-superior mesenteric artery, 27-distal abdominal aorta cannulation ligature, 28-abdominal aorta, 29-superior and superior hepatic vena cava, 30-liver, 31-proper hepatic artery, 32-splenic vein, supra mesenteric Confluence of vein and portal vein, 33-renal artery, 34-inferior vena cava, 42-temperature controller, 43-lifting column, 46-oxygen cylinder, 54-oxygenator and thrombus filter housing, 58-organ compartment Shelf, 60- return line, 63- oxygenator heating circulation interface, 64- vascular cannula connection, 65- oxygenator heater, 66- flow sensor, 67- controller, 68- temperature sensor, 69 -Pressure sensor, 70-perfusion pipeline clamp valve, 71-oxygenator heating circulation pump, 72-spleen, 73-stomach, 74-intestine, 75-kidney, 76-portal vein, 77-splenic vein, 78-mesentery Superior vein, 79-inferior mesenteric vein, 80-right gastroepiploic vein, 81-inferior mesenteric artery, 82-low voltage DC power supply, 83-control panel and display screen, 84-alarm device, 85-bubble sensor.

detailed description

The embodiments of the present invention are described below with reference to the drawings, in which the same components are denoted by the same reference numerals. In the case of no conflict, the following embodiments and technical features in the embodiments can be combined with each other.

The first embodiment

As shown in Figures 1-3, the device of the present invention is used in a biological organ 1. The biological organ 1 includes a biological organ and blood vessels attached to the biological organ. The biological organ 1 can be an organ cluster or a single organ .

The device of the present invention is used for mechanical perfusion and preservation of biological organs 1, and includes: organ bin 2, liquid storage bin 3, temperature maintaining device 4, oxygenator 5, plug filter 6, pump 7 and perfusion pipeline 8. Preferably, a blood vessel cannula 9 at the end of the perfusion line 8 is included.

The organ bin 2 is used to contain the biological organ 1 and the blood vessels attached to the biological organ; the liquid storage bin 3 is located at the lower part of the organ bin 2 and is used to collect the circulating perfusion fluid returning from the venous end of the biological organ; the temperature maintaining device 4 It is used to maintain the temperature of the body organ 1, the organ compartment 2, the liquid storage compartment 3 and the overall device; the oxygenator 5 is used to oxygenate the circulating perfusion liquid, the oxygenator 5 is connected with an oxygen cylinder 46; the plug filter 6 is used for Filters impurities such as thrombus and bubbles in the circulating perfusion solution; pump 7 is used for device perfusion power maintenance, pressurized perfusion and circulating perfusion; perfusion pipeline 8 is used to connect key components; blood vessel cannula 9 is located at the end of perfusion pipeline 8 , Connect the aortic system of the organ 1 for pressurized perfusion and circulatory perfusion. Preferably, the blood vessel cannula 9 has corresponding side holes at corresponding positions of different branches in the aortic system, and the inner diameter is matched with it, so that the corresponding organs can be perfused more accurately. The vascular cannula 9 may also have multiple branches.

The portal vein 76 does not need to be cannulated, and relies on the reflux perfusate perfused from the splenic artery 25 to the spleen 72, and the reflux perfusate perfused from the superior mesenteric artery 26 to the intestine 74. The perfusion is performed after the confluence of the splenic vein, superior mesenteric vein and portal vein 32 .

As shown in Figure 2, the basic working principle of the device is as follows: the biological organ 1 is placed in the organ compartment 2, and the temperature is maintained by the temperature maintenance device 4 outside the organ compartment 2 (the organ compartment 2 is placed in the temperature maintenance device 4), and the cycle The perfusion fluid (including blood or non-cellular circulating fluid, etc.) is refluxed and stored in the liquid storage tank 3, which is located below the organ tank 2. The organ bin shelf 58 of the organ bin 2 has through holes, so that the organ bin 2 and the liquid storage bin 3 are physically connected, so that the perfusion fluid flows out from the biological organ 1 and flows back to the liquid storage bin 3 in the lower layer of the organ bin. In one embodiment (as shown in Figures 6 and 7), the temperature maintaining device 4 is in the form of a water bath box 10, which can not only directly heat the organ compartment 2 immersed in the water bath box 10, but also has an oxygenator heating cycle The pump 71 (installed inside or outside the water bath tank 10) pumps out the warm water in the water bath tank 10, and circulates heating of the oxygenator 5, thereby improving the heating efficiency. In another embodiment, the temperature maintaining device 4 uses compressors, ice cubes or ultra-low temperature agents to maintain the low temperature of the liquid storage tank 3, the organ tank 2 and the biological organs 1 therein.

Driven by the pump 7, the circulating perfusate in the liquid storage tank 3 enters the thrombus filter 6 to filter out impurities and bubbles; then it passes through the oxygenator 5 for oxygenation; and then passes through the circulation line 8, the vascular cannula 9. Enter the aortic system of the organ 1 in the organ warehouse 2, and then use different branches of the aorta to simultaneously perfuse multiple organs; finally, the circulating perfusion fluid flows out through the venous end of the organ 1 and is collected The backflow is concentrated in the liquid storage tank 3, so that cyclic perfusion is formed.

The sequence of circulation is: Organ 1 in organ compartment 2—liquid storage compartment 3—thrombus filter 6—pump 7—oxygenator 5—perfusion line 8 (vascular cannula 9)—organism in organ compartment 2 Organ 1. The perfusion pipeline 8 connects the main components.

The organ preservation device of the present invention simultaneously perfuses multiple organs through the abdominal aorta or thoracic aorta system, in which the liver portal vein 76 does not need to be cannulated, and is perfused with the circulating perfusate returned from the portal vein system. The pulmonary artery does not need to be cannulated and is perfused with the circulating perfusion fluid of the right heart.

Optionally, the vascular cannula 9 is located at the end of the perfusion pipeline 8 and is connected to the accessory blood vessels of the organ 1 for perfusion.

Optionally, the blood vessel cannula 9 has corresponding side holes at corresponding positions of different branches in the aortic system, and the inner diameter is matched with it, so that the corresponding organs can be perfused more accurately.

As shown in Figure 3, when using this device, first, the biological organ 1 and its accessory blood vessels are placed in the organ compartment 2, and all organs are arranged in accordance with the normal anatomical structure. Then, both ends of the abdominal aorta 28 are ligated by the proximal abdominal aorta blocking ligature 23 and the distal abdominal aortic blocking ligature 27, respectively, and the circulation line 8 (optionally, the end is connected to a blood vessel cannula 9) After insertion into the abdominal aorta 28, it is fixed by a ligature. The circulating perfusate is driven and pressurized by the pump 7, and is injected into the abdominal aorta 28 through the circulation line 8 (optionally connected to the vascular cannula 9 at the end), and then perfusion of various organs through the branches of the abdominal aorta. Among them, the bilateral kidney 75 is perfused through the renal arteries 33; the liver 30 is perfused through the 24 branches of the abdominal trunk proper hepatic artery 31; the spleen 72 is perfused through the 24 branches of the abdominal trunk splenic artery 25; and the spleen 72 is perfused through the superior mesenteric artery 26 The inferior mesenteric artery 81 is arterially perfused into the intestine 74; the venous return of the intestine 74 is the superior mesenteric vein 78 and the inferior mesenteric vein 79, the venous return of the spleen 72 is the splenic vein 77, and the venous return of the stomach 73 is the right gastromental vein 80, The splenic vein, superior mesenteric vein, and portal vein converge together at the confluence 32, and the liver is perfused through the portal vein 76, so the portal vein 76 can be perfused without intubation. The device can perfuse the abdominal aorta 28 and its branches only through a single tube to complete the perfusion of the entire abdominal organs. All the circulating perfusate flows back to the organ compartment 2 and the reservoir 3 through the superior and inferior hepatic vena cava 29 and the inferior inferior vena cava 34 of the inferior vena cava system, and then the circulating perfusion solution in the reservoir 3 is driven by the pump 7 Enter the thrombus filter 6 to filter out impurities and bubbles; then pass through the oxygenator 5 for oxygenation; and then pass through the circulation line 8 (optionally connected to the vascular cannula 9 at the end) to enter the organism in the organ compartment 2 The aortic system of body organ 1, then uses different branches of the aorta to simultaneously perfuse multiple organs, and so on;

The device can simultaneously perfuse multiple thoracic organs through the thoracic aortic system, wherein the pulmonary artery does not need to be intubated, and is perfused through the circulating perfusion fluid of the right heart.

Optionally, the device of the present invention can be used for extracorporeal mechanical perfusion to preserve human organs, for multi-organ preservation, to play its key mutual protective effect, to preserve and to repair and evaluate organ vitality.

Optionally, the device of the present invention uses animal organs such as pigs or sheep to be mechanically perfused outside of the body to restore organ vitality, simulate human organs to the greatest extent, and conduct medical training.

Second embodiment

The second embodiment of the present invention will be described below with reference to FIGS. 4-10 to further detail the structure and principle of the organ bin 2, the liquid storage bin 3, the temperature maintaining device 4, the oxygenator 5, and the plug filter 6.

As shown in FIG. 4, the bottom of the organ warehouse 2 is provided with an organ warehouse shelf 58 with a number of holes that communicate with the lower liquid storage warehouse 3 (see FIG. 2) to facilitate the perfusion circulation fluid to flow back to the liquid storage warehouse 3. A return line 60 is provided inside the organ bin 2, one end of the return line 60 leads to the liquid storage bin 3, and the other end is connected to the circulation line 8. Preferably, the bottom surface of the liquid storage tank 3 is inclined toward the inlet of the return line 60 to facilitate maintaining a high liquid level, which not only facilitates the collection of circulating fluid, but also prevents the return line 60 from inhaling air. The outer wall of the organ bin 2 is provided with an oxygenator heating circulation interface 63 for sucking warm water in the liquid storage bin 3 below the circulating organ bin 2 and heating the circulating perfusate through the oxygenator 5. The oxygenator heating circulation interface 63 is not connected to the organ compartment 2 but is connected to the water bath box 10 (detailed below).

The side wall of the organ compartment 2 is provided with a diaphragm suspension 14 for suspending the diaphragm 22 (see FIG. 1). The diaphragm suspension 14 is a protrusion protruding inward from the inner wall of the organ compartment 2, and multiple diaphragm suspensions 14 can be provided. The diaphragm hook 14 is used to fix and hang the diaphragm part of the biological organ 1 to maximize the anatomical position of the liver in the abdominal cavity.

As shown in Figures 5-7, the device of the present invention includes: organ compartment 2, water bath box 10 (as an optional method of temperature maintaining device 4), pump 7, oxygenator 5, filter plug 6, return line 60 , The vascular cannula connecting portion 64 and the vascular cannula 9. Among them, the return end of the perfusion line 8 is connected to the return line 60, the perfusion end is connected to the vascular cannula connecting portion 64 and the vascular cannula 9, and the middle of the perfusion line 8 is connected to components such as pump 7, oxygenator 5, and thrombus filter 6. . The biological organ 1 is placed on the organ compartment shelf 58 in the organ compartment 2 and immersed in the water bath 10 to maintain the body temperature environment. The diaphragm hook 14 is used to fix and hang the diaphragm of the biological organ 1 to maximize the restoration of the abdominal anatomy position. Optionally, the organ shelf 58 of the organ compartment 2 may be made of metal, which serves as the negative plate of the high-frequency electrosurgical knife 15 in order to use the high-frequency electrosurgical knife 15.

The circulating perfusate is driven by the pressure of the pump 7 and enters the abdominal aorta or thoracic aorta system of the organism 1 through the vascular cannula 9, and perfuses multiple organs in the abdominal or thoracic cavity at the same time. The portal vein 76 does not need to be intubated and depends on the spleen The arterial 25 is infused into the reflux perfusion fluid of the spleen 72, and the superior mesenteric artery 26 is perfused into the intestinal intestinal 74. The perfusion is performed after the confluence of the splenic vein, superior mesenteric vein and portal vein 32. The pulmonary artery does not need to be cannulated and is perfused with the circulating perfusion fluid of the right heart. Thereafter, the circulating perfusate flows out through the inferior vena cava of the biological organ 1, and flows back to the reservoir 3 in the lower part of the organ bin 2 through the porous structure of the organ bin shelf 58; then, driven by the pump 7, the reservoir The circulating fluid in 3 is drawn out through the return line 60 to flow to the plug filter 6 to filter out the thrombus and air clots mixed in the circulating fluid; then through the outlet of the plug filter 6 through the perfusion line 8 through the pump 7, and then through the perfusion The pipeline 8 enters the oxygenator 5 to oxygenate the circulating fluid; then enters the vascular cannula connection portion 64 from the outlet of the oxygenator 5, and then enters the vascular cannula 9 through the vascular cannula connection portion 64, thus completing the cycle reciprocally Perfusion process.

As shown in FIGS. 6-9, the organ bin 2 and the liquid storage bin 3 are placed in a temperature maintaining device 4, and the temperature maintaining device 4 includes a water bath box 10 and a temperature controller 42. There are two ways to maintain the temperature. In the first way, warm water is contained in the water bath 10 to directly heat the organ compartment 2, the liquid storage compartment 3, and the biological organ 1. The second way is to set the oxygenator heating circulation pump 71 to suck the warm water in the water bath tank 10 through the oxygenator heating circulation interface 63 into the oxygenator heater 65, which is located in the oxygenator. Below the device 5 to heat the circulating fluid in the oxygenator 5.

The temperature controller 42 can control the temperature of the water bath box 10, and the temperature controller 42 can control the electric heating device to heat the water in the water bath box 10.

The oxygenator 5 and the plug filter 6 are centrally placed in the housing 54 of the oxygenator and plug filter, and the corresponding circulation pipelines 8 are regularly distributed in the housing 54 for quick installation and replacement; optionally, except for the pump 7 , The organ warehouse 2, the liquid storage warehouse 3, the oxygenator 5, the thrombus filter 6, the perfusion pipeline 8, the vascular cannula 9, the return pipeline 60 and other components can be used for one time, which is convenient for quick replacement.

Referring again to FIG. 6, the temperature sensor 68 is arranged inside the organ compartment 2 for monitoring the temperature of the biological organ 1 in the organ compartment 2. The flow sensor 66 is arranged on the return line 60 and the vascular cannula connection portion 64 for monitoring the flow of the perfusion fluid circulating through the return line 60 and the vascular cannula connection portion 64. The pressure sensor 69 is arranged on the blood vessel cannula connection portion 64, wherein the blood vessel cannula 9 is connected to the end of the blood vessel cannula connection portion 64. The pressure sensor 69 is used to monitor the pressure at the vascular cannula connection portion 64, that is, to monitor the pressure of the circulating perfusate to be delivered to the vascular cannula 9. The air bubble sensor 85 is used to monitor air bubbles in the perfusion line 8.

6 again, the organ perfusion preservation device of the present invention also includes a perfusion line clamp valve 70, which is arranged on the perfusion line 8. The figure shows that it is arranged near the input end of the pump 7. The perfusion line clamp valve 70 can be closed. The circulatory perfusion of the perfusion line 8 is quickly stopped to avoid damage to the biological organ 1 when abnormalities occur.

As shown in FIG. 10, the organ perfusion preservation device of the present invention further includes an electronic control system, and the electronic control system is powered by a low-voltage DC power supply 82. The electronic control system includes a controller 67, a control panel and a display screen 83. Figure 10 shows the schematic circuit diagram of the electronic control system. Among them, the device of the present invention may be equipped with a lifting column 43, which is installed under the liquid storage bin 3, and can raise the height of the liquid storage bin 3 (together with the organ bin 2). The device of the present invention also includes a temperature sensor 68, a flow sensor 66, a pressure sensor 69, and a bubble sensor 85. After the signals of the sensors are transmitted to the controller 67 for analysis and processing, the controller 67 controls the temperature of the temperature maintaining device 4 and controls the pump 7. To prevent abnormal perfusion pressure or flow rate, control the opening and closing state of the perfusion pipeline clamp valve 70, and provide timely alarm through the alarm device 84 when an abnormal situation occurs in the device. The alarm can be displayed on the control panel and the display 83.

As shown in Fig. 11, the system of the present invention also has a bile collection and measurement device 16 and a urine collection and measurement device 17 for collecting bile and urine and dynamically observing organ vitality.

The above-mentioned embodiments are only preferred specific implementations of the present invention, and the usual changes and substitutions made by those skilled in the art within the scope of the technical solution of the present invention should be included in the protection scope of the present invention.

Claims

A mechanical perfusion preservation device for biological organs, which is characterized in that it comprises:

Organ warehouse (2): used to house the organs and accessory blood vessels;

Liquid storage bin (3): located below the organ bin (2), used to store circulating perfusion fluid;

Temperature maintenance device (4): used to maintain the temperature of biological organs and circulating fluid;

Oxygenator (5): used to continuously oxygenate the circulating perfusate;

Plug filter (6): used to filter circulating perfusate;

Pump (7): pressurize the circulating perfusate;

Perfusion pipeline (8): connect the reservoir (3), the plug filter (6) and the oxygenator (5) in sequence, and pressurize the biological organs under the drive of the pump (7). Circulation perfusion

The mechanical perfusion preservation device can pressurize and circulate the aortic system of an organism, and can perfuse a single or multiple organism organs at the same time.
The perfusion preservation device according to claim 1, wherein:

The mechanical perfusion preservation device can simultaneously perfuse multiple abdominal organs through the abdominal aortic system, wherein the liver portal vein (76) does not need to be cannulated, and is perfused with the circulating perfusion fluid returned from the portal vein system.
The perfusion preservation device according to claim 1, wherein:

The mechanical perfusion preservation device can simultaneously perfuse multiple thoracic organs through the thoracic aortic system, wherein the pulmonary artery does not need to be intubated, and is perfused with the circulating perfusion fluid of the right heart.
The perfusion preservation device according to claim 1, further comprising:

The blood vessel cannula (9) is located at the end of the perfusion pipeline (8) and is used to connect the accessory blood vessels of the biological organ (1) for perfusion.
The perfusion preservation device according to claim 4, wherein:

The blood vessel cannula (9) has a corresponding side hole, and the inner diameter of the side hole is matched with the corresponding positions and inner diameters of the different branches of the blood vessel cannula (9) in the aortic system.
The perfusion preservation device according to claim 1, wherein:

The temperature maintaining device (4) is a water bath box (10), and the organ warehouse (2) and the liquid storage warehouse (3) are placed in the water bath box (10).
The perfusion preservation device according to claim 6, further comprising:

The oxygenator heating circulation pump (71) is used for pumping out warm water in the water bath (10) to heat the oxygenator (5) in circulation.
The perfusion preservation device according to claim 6, further comprising:

The temperature maintaining device (4) includes a temperature controller (42), and the temperature controller (42) can control the temperature of the water bath (10).
The perfusion preservation device according to claim 1, further comprising:

The temperature maintaining device (4) is a compressor, ice cubes or ultra-low temperature agent, which is used to maintain the low temperature of the liquid storage tank (3), the organ tank (2) and the biological organs.
The perfusion preservation device according to claim 6, further comprising:

The temperature sensor (68) is used to monitor the temperature of the circulating perfusion fluid and the biological organs in the liquid storage tank (3);

A flow sensor (66) for monitoring the flow of the circulating perfusion liquid in the perfusion pipeline (8);

A pressure sensor (69) for monitoring the pressure of the circulating perfusion liquid in the perfusion pipeline (8);

The bubble sensor (85) is used to monitor whether there are bubbles in the perfusion pipeline (8).
The perfusion preservation device according to claim 10, further comprising:

The controller (67) collects the signal of the temperature sensor (68) and controls the temperature maintaining device (4) based on the signal.
The perfusion preservation device according to claim 10, further comprising:

The controller (67) collects the signals of the flow sensor (66) and the pressure sensor (69), and controls the pump (7) based on the signals.
The perfusion preservation device according to claim 10, further comprising:

The controller (67) collects the signals of the flow sensor (66), the pressure sensor (69) and the air bubble sensor (85), and controls the perfusion line clamp valve (70) based on the signals (70) The filling of the filling line (8) can be stopped.
The perfusion preservation device according to claim 1, wherein:

The bottom of the organ bin (2) is provided with an organ bin shelf (58), and the organ bin shelf (58) has a through hole communicating with the liquid storage bin (3).
The perfusion preservation device according to claim 1, further comprising:

The bile collecting and measuring device (16) for collecting bile and the urine collecting and measuring device (17) for collecting urine are used to dynamically observe the vitality of the biological organ (1).