CN201276574Y - Cell record slot suitable for diaphragm plier recording technique - Google Patents
Cell record slot suitable for diaphragm plier recording technique Download PDFInfo
- Publication number
- CN201276574Y CN201276574Y CNU2008201914039U CN200820191403U CN201276574Y CN 201276574 Y CN201276574 Y CN 201276574Y CN U2008201914039 U CNU2008201914039 U CN U2008201914039U CN 200820191403 U CN200820191403 U CN 200820191403U CN 201276574 Y CN201276574 Y CN 201276574Y
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- groove
- cell
- weir
- chamber
- salt bridge
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Abstract
The utility model relates to a cell recording chamber for patch clamp recording technique; the cell recording chamber comprises a chamber body, a cell chamber, a glass slide, a falf groove, a salt bridge placing hole, a silver column, a silver plate, a drainage pore channel, a drug feeding channel and a drug-adding groove; the cell chamber is arranged at the center of the chamber body, and the glass slide is used as a chamber bottom, and the falf groove is expanded at the circumstance of the upper part of the cell chamber; the drainage pore channel at the chamber bottom is arranged at one end of the falf groove, and the drug feeding channel and the drug-adding groove are arranged at the other end thereof; perfusion liquid is filled from the drug-adding groove and flows into the cell chamber along the falf groove wall by the drug feeding channel; the salt bridge placing holes are respectively arranged inside and outside the falf groove, and the salt bridge placing hole outside the falf groove penetrates through the chamber body up and down, and an L-shaped silver plate is pasted on the side surface and bottom surface, and a lower end opening of the salt bridge placing hole outside the falf groove is sealed. The cell recording chamber has the advantages of scientific design, simple structure, small noise disturbance, good transmittance and heat insulating ability, convenient liquid flowing and exchange, easy deslagging, easy cleaning and good bargain, therefore, the chamber is suitable for being popularized and applied in the research area of the patch clamp.
Description
Technical field
The utility model relates to a kind of cell record groove that is applicable to patch clamp recording.
Background technology
The cell record groove is to manufacture and design for carrying out of matching film plate tongs technology, its purposes provides after viable cell to acute isolation, culturing cell, the instantaneous tissue slice of live body etc. be placed in one, can continue to keep the place of a long-time survival, make patch clamp clamp down on cell processes and finished smoothly.And, the cell record groove still is provided as to be kept the long-time survival of active somatic cell and is added on the place that extracellular fluid exchanges, this exchange is to close the different requirements of symbol by the active somatic cell or the section injection of attached chemicals dosing plant in the cell record groove, through oxygen-saturated extracellular fluid, perhaps inject the extracellular fluid of the pharmaceutical cpd that is mixed with different concns, after abundant function cells, by the accessory vacuum pump extracellular fluid sucking-off too much in the cell record groove, but keep a certain amount of extracellular fluid again in track, allow cell or section are heavy to be attached to the cell record trench bottom and not to be washed away by perfusate, be convenient to experimenter's pair cell or section and carry out patch clamp experiments.The cell record groove also should become suitable accessory heating system track is carried out heat tracing, keeps the biography temperature conductor of the psychological need of active somatic cell in the cell record groove.The cell record groove of selling on the market although seem simple, mostly is imported product at present, costs an arm and a leg, and a cell record groove adds that the adapter that matches needs the hundreds of U.S. dollar; On the other hand, that these import cell record grooves exist is big as noise jamming, liquid flow and exchange is inconvenient, different separately defective such as easy cleaning not, can not reach the above-mentioned various requirement of mentioning fully.For overcoming defective and the deficiency that present commercially available cell record groove exists, need a kind of new cell record groove that is applicable to patch clamp recording of research and development.
Summary of the invention
The purpose of this utility model provides a kind of simplicity of design, random with material, convenient, the easy removal of impurity of volume size mobile and exchange suitable, that can significantly reduce noise jamming, transparence and high insulating effect, liquid, the easy cleaned cheap and good-quality cell record groove that is applicable to patch clamp recording.
In order to realize the purpose of this utility model, a kind of cell record groove that is applicable to patch clamp recording is provided, comprise cell body (1), cell groove (2) and dosing tank (10), there is the cell groove in central authorities at cell body, the cell groove is a bottom land with slide glass (3), expanding around on cell groove top has a weir to enclose groove (4), there is drainage duct (8) on the weir at the bottom of enclosing an end slot of groove, the other end that encloses groove on the weir has into medicine passage (9), advance the medicine passage and be communicated with dosing tank (10), perfusate is injected into dosing tank and encloses cell wall along the weir and flow in the cell groove through advancing the medicine passage; In groove is enclosed on the weir with the cell groove outside one jiao a salt bridge holding hole (5) is arranged, the cell body limit also has a salt bridge holding hole (5) that runs through cell body outside groove is enclosed on the weir, silver plate (7) is L shaped side and the bottom surface of pasting at cell body, and the salt bridge holding hole lower port on the outer cell body limit of groove is enclosed on the sealing weir.
The utility model is applicable to the cell record groove of patch clamp recording, and described cell body (1) adopts polyethylene clear plastics plate to make.For with the inverted microscope Stage microscope on the clamping adapter supporting, be convenient to the clamping, fixing of clamper pair cell track, and the size requirements of track is consistent with the heating adapter of cell radiator valve, therefore selects the long 70mm of cell body, wide 50mm, thick 7mm; If track is too dark, will stop moving of patch clamp recording electrode, because the miles of relative movement of patch clamp recording electrode is to move under the control of three-dimensional micromanipulator, each only is that 20 μ m left and right sides modification scopes are very little for the top to bottom, left and right, front and rear miles of relative movement of three-dimensional narishige, no matter be all around, move up and down electrode, miles of relative movement is very limited.
The utility model is applicable to the cell record groove of patch clamp recording, and described cell groove (2) is to be central point with cell body (1) central authorities, and the rectangular parallelepiped that cuts out long 20mm, wide 15mm, dark 7mm forms cell wall, is the groove that bottom land surrounds with slide glass (3) again.Dig out a shallow slot in the bottom surface of cell body, shallow slot is polished into frosting, and slide glass is pasted in shallow slot by seal gum or medical ventolin; The length of shallow slot and width exceed cell bottom land length and width, and the long limit of shallow slot extends to the limit portion of cell body, make the cell groove neither can seepage liquid, and slide glass can not protrude in the cell body bottom surface again, helps track and lies on the microscope stage.Adopt slide glass to make the bottom land of cell groove, finish experiment at every turn after, remove slide glass, track is cleaned, change a new slide glass again when doing experiment and get final product next time, and such design not only makes things convenient for track to clean but also helps can also guaranteeing transparence to the track heat tracing.The used slide glass of the utility model is commercially available medical slide glass.
The utility model is applicable to the cell record groove of patch clamp recording, described weir enclose groove (4) be on cell groove top to around expand out a long 30mm, wide 25mm, apart from the groove of the dark 3.5mm in cell body surface.At the bottom of groove one end slot is enclosed on the weir, the drainage duct (8) that to bore a diameter be 2mm, the drainage channel diameter can just inject the vapor pipe syringe needle of infusing that is used to that uses clinically, and purpose is to discharge the weir to enclose perfusate in the groove; Enclose the other end of groove on the weir, into medicine passage (9) is arranged, advance medicine passage lower-most point and enclose bottom land 1mm from the weir, advancing the medicine passage is communicated with dosing tank (10), dosing flute length 10mm, the dark 2mm of wide 5mm, perfusate is injected into dosing tank by attached chemicals dosing plant, and advancing the medicine passage is narrow irrigation canals and ditches, the perfusate of dosing tank is guided to the weir enclose groove and flow in the cell groove along cell wall.Allow perfusate very slowly flow in the cell groove like this, can avoid perfusate directly to inject the cell groove and cause the disadvantage that the viable cell that is attached to the cell trench bottom is washed away.
The utility model is applicable to the cell record groove of patch clamp recording, described salt bridge holding hole (5) is a hole of putting salt bridge, outside the cell groove and the weir enclose corner in the groove circular hole that to be drilled with a diameter be 5mm, another weir in its same side encloses outside the groove, from 2mm place, cell body edge, diameter 5mm, run through cell body hole up and down, two holes are the salt bridge holding hole, and salt bridge straddles in these two holes.
The utility model is applicable to the cell record groove of patch clamp recording, and described silver plate (7) is L shaped, is welded with a silver-colored post (6) on silver plate; Polish a little shallow slot around the salt bridge holding hole of cell body bottom surface, through frosted, and paste thin silver plate (7), the silver plate size covered the lower port of salt bridge holding hole fully, and silver plate had both played ground connection acting on, and did not allow salt bridge holding hole seepage liquid again.When using the cell record groove, earthing rod links to each other by the metal filamentary silver on silver post and the patch clamp electrode jaw, inverted U-shaped salt bridge straddles in two salt bridge holding holes, form the good conducting system that salts solution is cascaded in a ground wire by electrode jaw, silver plate, salt bridge, the cell record groove, can significantly reduce of the interference of the noise of salts solution generation in the track patch clamp record result.
The utility model encloses cell wall by the weir, cell cell wall and slide glass are encircled into the bath of receivability solution, bath lower floor volume is effective track, the upper strata is that groove is enclosed on the weir, into medicine passage (9) and drainage duct (8) are arranged on the upper strata, when contain the viable cell suspension join bath and do of short duration static after, with the accessory chemicals dosing plant extracellular fluid is passed through dosing tank (10), be injected into the weir and enclose groove through advancing the medicine passage, enclose cell wall along the weir and slowly flow to effective track, after filling with effective track and weir and enclose groove Deng liquid, because being easy attached wall, myocardial cell's characteristic of living inhales the end, static wait viable cell to attach to lower floor effective track bottom for a moment after, the weir is enclosed that mixed solution aspirates away the groove in the drainage duct of enclosing groove from the weir with constant flow pump or vacuum pump, up to keeping the effective solution amount of lower floor, this process is through carrying out repeatedly, by injecting extracellular fluid, process to the outer liquid one-in-and-one-out of suction of cells, just can wash away solution impurities in the bath, the clean degree that keeps solution in the bath helps pair cell or section and carries out patch clamp and clamp down on.
The cell record groove bath volume of the utility model design is 1.0～1.5ml, and volume size is suitable.If the bath volume is too big, the electrolyte solution that is contained in the cell record groove will produce very big capacitance resistance, and capacitance resistance is excessive, will produce noise jamming, thereby has influenced the command potential of patch clamp.Because carry out patch clamp when clamping down on when pair cell or section, produce very big liquid junction potential in the bath between electrolyte solution and recording electrode, this big liquid junction potential has often surpassed the compensation range of patch clamp amplifier, so that patch clamp can't be carried out.Certainly, the volume of bath can not be too little, otherwise the liquid in the bath is very easy to be evaporated, and makes heavy to be attached to the cell of effective track bottom or cut into slices nutrition for want of and oxygen and death.
The utility model is applicable to that the cell record groove of patch clamp recording has following advantage:
1, the utility model simplicity of design is random with material, compares with the import cell record groove of selling in the market, and price is very low.
2, the cell body thickness of the utility model design is suitable, and the volume size strictness is designed to the specification of 1.0～1.5ml by requirement of experiment, has increased the operability of patch clamp recording electrode greatly, and has reduced the noise jamming that liquid junction potential produces.
3, the design of the utility model salt bridge holding hole is ingenious, reaches salt bridge holding hole lower port and pastes a thin silver plate, and the ground connection effect is very good, and the noise jamming of generation is especially little.
4, the utility model is expanded the weir to enclose groove is very reasonable and necessary on cell groove top, can exchange mobile perfusate in the bath, siphon away liquid impurity too much in the bath, can not irritate liquid because of exchange again influences the viable cell that is attached to effective track bottom.
5, the utility model adopts slide glass to paste at the cell groove back side as bottom land, like this can very convenient cleaning cell record groove, each only the need thoroughly be cleaned cell body, change clean slide glass then and get final product, and the printing opacity of slide glass and temperature conduction effect is fine.
Description of drawings
The utility model is described further with accompanying drawing:
Fig. 1 is the schematic top plan view of the utility model cell record groove overall structure.
Among the figure, (1) cell body, (2) cell groove, (3) slide glass, (4) weir are enclosed groove, (5) salt bridge holding hole, (6) silver-colored post, (7) silver plate, (8) drainage duct, (9) and are advanced medicine passage, (10) dosing tank.
Fig. 2 looks diagrammatic cross-section for the master of the utility model cell record groove structure.
Fig. 3 is the utility model cell record groove rear side viewing synoptic diagram.
Embodiment
Embodiment 1: the utility model be applicable to patch clamp recording the cell record groove structure as shown in Figure 1.Include cell body (1), slide glass (3), silver-colored post (6) and silver plate (7), have cell groove (2), weir to enclose groove (4), two salt bridge holding holes (5), drainage duct (8) on the cell body, advance medicine passage (9) and dosing tank (10).
The volume of the utility model cell record groove receivability solution is 1.5ml, and making step is as follows:
1. cell body is selected polyethylene clear plastics plate for use, makes the cell body of long 70mm, wide 50mm, thick 7mm;
2. cut out the rectangular parallelepiped of long 20mm * 15mm * 7mm earlier in cell body central authorities,, expand out a long 30mm, wide 25mm all around, enclose groove from the weir of the dark 3.5mm in cell body surface from mediad on the cell body top that cuts out rectangular parallelepiped;
3. enclose the mid point on groove one end slot base on the weir, the drainage duct (8) that to bore a diameter be 2mm; The other end that encloses groove on the weir digs the dosing tank (10) of a long 10mm, wide 5mm, dark 2mm, opens one again from the cell body surface and advances medicine passage (9) from what dosing tank led to that the weir encloses groove, advances medicine passage lower-most point and encloses groove bottom land 1mm from the weir; Be a narrow open drain canal, can guide to the weir to the liquid that advances the medicine groove and enclose in the groove.
4. at the cell body back side, dig one with the commercially available the same thick and wide shallow slot of medical slide glass, shallow slot length and width exceed the length and the width of the rectangular parallelepiped that cuts out, the long limit of shallow slot extends to the cell body limit always, shallow slot is polished into frosting;
5. bore two salt bridge holding holes (5): outside the cell groove and the weir enclose 5mm circular hole of an angular bit in the groove, a 5mm hole is also bored from 2mm place, cell body edge in the same side outside groove is enclosed on the weir, hole runs through about the cell body;
6. enclose the shallow slot that digs a 20mm * 12mm around the salt bridge holding hole outside the groove on the cell body back side, weir, shallow slot is polished into frosting, paste in the side and bottom surface shallow slot of cell body with L shaped silver plate, make silver plate closed allround cell body bottom surface salt bridge holding hole, guarantee the non-leakage liquid of salt bridge holding hole; Weldering one silver medal post (6) on silver plate;
7. with commercially available medical slide glass (3) with seal gum paste the shallow slot at the cell body back side in, and guarantee the non-leakage liquid of cell groove.The utility model cell record groove has just completed.
Embodiment 2: make the cell record groove that the utility model is suitable for patch clamp recording, and its basic structure such as embodiment 1, difference is: volume is 1.0ml.Its two drainages duct (8) is not located at the weir and encloses center, bottom land limit, encloses the nearly corner in bottom land limit and be located at the weir; Its three slide glass (3) with medical ventolin directly paste the shallow slot at the cell body back side in.
The utility model cell record groove that the foregoing description 1 and embodiment 2 make, good and cheap, volume size suits, when transparence and good heat preservation performance, use liquid flow and exchange is convenient, the easily removal of impurity, be easy to clean, can significantly reduce noise jamming, increase the operability of patch clamp recording electrode greatly.
The utility model is applicable to advantages such as the cell record groove of patch clamp recording has design science, simple in structure, and is easy to use and good and cheap, is suitable for the research field promotion and application at patch clamp technique.
Claims (4)
1, a kind of cell record groove that is applicable to patch clamp recording, comprise cell body, cell groove and dosing tank, it is characterized in that the cell groove being arranged in the central authorities of cell body, the cell groove is bottom land with the slide glass, expanding around on cell groove top has a weir to enclose groove, and there is the drainage duct on the weir at the bottom of enclosing an end slot of groove, and the other end that encloses groove on the weir has into medicine passage, advance the medicine passage and be communicated with dosing tank, perfusate is injected into dosing tank and encloses cell wall along the weir and flow in the cell groove through advancing the medicine passage; In groove is enclosed on the weir with the cell groove outside one jiao a salt bridge holding hole is arranged, the cell body limit also has a salt bridge holding hole that runs through cell body outside groove is enclosed on the weir, silver plate is L shaped side and the bottom surface of pasting at cell body, and the salt bridge holding hole lower port on the outer cell body limit of groove is enclosed on the sealing weir.
2, the cell record groove that is applicable to patch clamp recording according to claim 1 is characterized in that digging a shallow slot in the bottom surface of cell body, and shallow slot is polished into frosting, and slide glass is pasted in shallow slot by seal gum or medical ventolin.
3, the cell record groove that is applicable to patch clamp recording according to claim 1 is characterized in that silver plate is L shaped, is welded with a silver-colored post on silver plate.
4, the cell record groove that is applicable to patch clamp recording according to claim 1, the volume that it is characterized in that bath is 1.0～1.5ml.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008201914039U CN201276574Y (en) | 2008-10-13 | 2008-10-13 | Cell record slot suitable for diaphragm plier recording technique |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008201914039U CN201276574Y (en) | 2008-10-13 | 2008-10-13 | Cell record slot suitable for diaphragm plier recording technique |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201276574Y true CN201276574Y (en) | 2009-07-22 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNU2008201914039U Expired - Fee Related CN201276574Y (en) | 2008-10-13 | 2008-10-13 | Cell record slot suitable for diaphragm plier recording technique |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201276574Y (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108490165A (en) * | 2018-03-21 | 2018-09-04 | 厦门大学附属第医院 | Constant temperature patch-clamp cell record system |
| CN111321052A (en) * | 2018-12-14 | 2020-06-23 | 中国科学院深圳先进技术研究院 | Cell recording groove device and cell recording method |
-
2008
- 2008-10-13 CN CNU2008201914039U patent/CN201276574Y/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108490165A (en) * | 2018-03-21 | 2018-09-04 | 厦门大学附属第医院 | Constant temperature patch-clamp cell record system |
| CN111321052A (en) * | 2018-12-14 | 2020-06-23 | 中国科学院深圳先进技术研究院 | Cell recording groove device and cell recording method |
| CN111321052B (en) * | 2018-12-14 | 2023-09-26 | 中国科学院深圳先进技术研究院 | Cell recording groove device and cell recording method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20090722 Termination date: 20111013 |