CN110157599A - A kind of culture device of pilot scale culture umbilical cord mesenchymal stem cells - Google Patents
A kind of culture device of pilot scale culture umbilical cord mesenchymal stem cells Download PDFInfo
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- CN110157599A CN110157599A CN201910618105.6A CN201910618105A CN110157599A CN 110157599 A CN110157599 A CN 110157599A CN 201910618105 A CN201910618105 A CN 201910618105A CN 110157599 A CN110157599 A CN 110157599A
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- working chamber
- holding tray
- putting hole
- sliding
- block
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/24—Heat exchange systems, e.g. heat jackets or outer envelopes inside the vessel
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Abstract
The invention discloses a kind of culture devices of pilot scale culture umbilical cord mesenchymal stem cells, including shell and it is set to working chamber in the shell and to open front, holding tray moving up and down is equipped in the working chamber, upper and lower perforative putting hole is equipped in the holding tray, it will be in cryopreservation tube placement and the putting hole when use, closing cap is set to be located at the holding tray upper surface, the heat block being located on the downside of the holding tray is equipped in the working chamber, it is symmetrical arranged before and after the heat block, the heat block can move forward and backward, thawing vat is equipped in the heat block, stem cell can be carried out batch and thawed simultaneously by the present invention, defrosting mechanism is separated with cryopreservation tube automatically after reaching setting time, to improve defrosting efficiency and defrosting quality, advantage is created for culture later, and, after the completion of defrosting, the present invention Cryopreservation tube can be opened automatically in batches, subsequent step is facilitated to carry out, shorten culture time.
Description
Technical field
The present invention relates to stem cell culture device technical field, specially a kind of pilot scale culture umbilical cord mesenchymal stem cells
Culture device.
Background technique
Stem cell has huge on cell therapy, organizational engineering and regenerative medicine and drug and biotechnology applications
Prospect, stem cell has the ability of self-renewing, and can be divided into specific cell type, however, stem cell is applied
Clinically need to use the stem cell of high quality and high quantity, this just needs the large-scale culture of stem cell, then effectively and equal
Even is divided into functional derivatives, and stem cell is generally stored in liquid nitrogen, needs to carry out quick-thawing before culture, and
Thawing time is unsuitable too long, and to be accomplished manually as this general step 1 in tradition, efficiency is lower, it is difficult to which batch thaws, Er Qienan
With accurate perception thawing time, keep subsequent culture effect bad, after the completion of stem cell is thawed, needs to open cryopreservation tube addition
Stem cell media, also to be accomplished manually as this step 1, it is difficult to realize that batch is opened, for disadvantages mentioned above, the present invention is carried out
It improves.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of culture device of pilot scale culture umbilical cord mesenchymal stem cells,
Overcome above-mentioned problem.
The present invention is achieved through the following technical solutions.
A kind of culture device of pilot scale culture umbilical cord mesenchymal stem cells of the invention, including shell and it is set to institute
The working chamber in shell and to open front is stated, holding tray moving up and down is equipped in the working chamber, is set in the holding tray
There is perforative putting hole up and down, cryopreservation tube is placed with the putting hole when use, be located at closing cap on the holding tray
Surface；
It is equipped with the heat block being located on the downside of the holding tray in the working chamber, is symmetrical arranged before and after the heat block, it is described to add
Heat block can move forward and backward, and thawing vat is equipped in the heat block, is fixed with constant temperature water pipe, the perseverance on the thawing vat inner wall
37 degree of warm water is housed in warm water tube, after the front and back heat block offsets, the cryopreservation tube is wrapped in the thawing vat
In, by the umbilical cord mesenchyma quick-thawing in the cryopreservation tube under constant temperature water pipe effect；
It is equipped with the lower holding tray being located on the downside of the holding tray in the working chamber, is equipped in the lower holding tray perforative up and down
Lower putting hole, the lower placement hole location is on the downside of the putting hole and concentric with the putting hole, is equipped in the lower putting hole
Annular chamber, rotation is equipped with swivel becket in the annular chamber bottom wall, and the swivel becket periphery is fixed with convex block, the annular chamber
It is connected to the lower putting hole, the annular chamber bottom wall is equipped with guide groove, and sliding is equipped with guide pad in the guide groove, described to lead
To connecting spring is connected between block and the guide groove inner wall, the guide pad upper end is fixed with compression bar, the press
Bar one end is fixed with the gripping block that may extend in the lower putting hole, and after the completion of defrosting, the holding tray drives the jelly
It deposits cryopreservation tube described in general who has surrendered under pipe to be inserted into the lower putting hole, the swivel becket rotation presses the top by the convex block
Compression bar extends to the gripping block in the lower putting hole to clamp the cryopreservation tube, and the holding tray, which rises, to be resetted, thus
The closing cap is separated with the cryopreservation tube.
Further, described working chamber or so wall is symmetrically arranged with upper hopper chute, and sliding is equipped with top shoe, institute in the upper hopper chute
It states and is connected with upper connecting rod between holding tray and the top shoe, the working chamber roof is equipped with upper motor, the upper motor
Downside has been connected by power screw shell, and threaded rod, the upper threaded rod lower end and institute are threaded in the screw shell
Holding tray upper surface is stated to be fixedly connected.
Further, the working chamber bottom wall rotation is equipped with gear shaft, and the gear shaft is fixedly connected with the swivel becket,
It is fixed with gear on the gear shaft, rack gear that is capable of sliding back and forth and engaging with the gear is equipped in the working chamber.
Further, the working chamber front and rear wall, which symmetrically rotates, is equipped with lower threaded rod, and the working chamber rear wall is fixed with
Lower motor, the lower motor are connected by power with the rear side lower threaded rod, pass through belt wheel mechanism between the lower threaded rod of front and back
Transmission connection, the front and back heat block are located remotely from each other end face and are fixed with mounting rack, the mounting rack and the lower threaded rod spiral shell
Line connection, the heat block left side is fixed with lower connecting rod, is symmetrically arranged with gliding groove before and after the working chamber left wall, described
Sliding is equipped with sliding block in gliding groove, and the lower connecting rod is fixedly connected with the sliding block.
Further, the working chamber left wall is equipped with first sliding groove, and sliding is equipped with the first sliding block, institute in the first sliding groove
It states and is connected with reset spring between the first sliding block and the first sliding groove antetheca, first sliding block right side is equipped with spring groove,
Sliding is equipped with first connecting rod in the spring groove, is connected with press bullet between the first connecting rod and the spring groove bottom wall
Spring, the first connecting rod right end are fixed with intermediate plate, are fixedly connected with intermediate bar between the intermediate plate and the rack gear,
The first connecting rod right side is fixed with driver plate, and sliding is equipped with positioning plate, the positioning plate lower end in the upper hopper chute
Face is fixed with the drive rod for extending downward into the working chamber and offseting with the driver plate upper surface, in the intermediate plate
Accommodating groove equipped with upward opening, sliding is equipped with ramp blocks in the accommodating groove, the ramp blocks and the storage groove bottom wall it
Between be connected with buffer spring.
Further, the gripping block is arc, and the gripping block is rubber material.
Further, there are four the putting hole is set, there are four the lower putting hole is set.
Beneficial effects of the present invention: it after umbilical cord mesenchymal stem cells are removed from liquid nitrogen, is placed in the present invention in batches
Stem cell can be carried out batch and thawed simultaneously by designated position, the present invention, reach after setting time defrosting mechanism automatically with freeze
Pipe is separated, to improve defrosting efficiency and defrosting quality, the culture after being creates advantage, also, is solving
After the completion of jelly, the present invention can in batches automatically be opened cryopreservation tube, and subsequent step is facilitated to carry out, when shortening culture preparation
Between.
Detailed description of the invention
It, below will be to embodiment or existing in order to illustrate more clearly of inventive embodiments or technical solution in the prior art
Attached drawing needed in technical description is briefly described, it should be apparent that, the accompanying drawings in the following description is only to invent
Some embodiments for those of ordinary skill in the art without creative efforts, can also basis
These attached drawings obtain other attached drawings.
Fig. 1 is overall structure of the present invention；
Fig. 2 is structural schematic diagram at A-A in Fig. 1；
Fig. 3 is structural schematic diagram at B-B in Fig. 1；
Fig. 4 is that C-C locates structural schematic diagram in Fig. 1；
Fig. 5 is structural schematic diagram at D-D in Fig. 1；
Fig. 6 is structural schematic diagram at E in Fig. 1.
Specific embodiment
Below with reference to Fig. 1-6, the present invention is described in detail, wherein for sake of convenience, now to hereafter described orientation
Provide as follows: hereafter described front-rear direction up and down is consistent with the front-rear direction up and down of Fig. 1 projection relation itself.
A kind of culture device of pilot scale culture umbilical cord mesenchymal stem cells in conjunction with described in attached drawing 1-6 mainly includes shell
It body 10 and is set in the shell 10 and is equipped with to the working chamber of open front 11, in the working chamber 11 moving up and down
Holding tray 26, is equipped with upper and lower perforative putting hole 18 in the holding tray 26, and when use places cryopreservation tube 27 and the placement
In hole 18, closing cap 17 is made to be located at 26 upper surface of holding tray；
The heat block 25 for being located at 26 downside of holding tray is equipped in the working chamber 11,25 front and back of heat block is symmetrically set
It sets, the heat block 25 can move forward and backward, and thawing vat 29 is equipped in the heat block 25, and fixation is set on 29 inner wall of thawing vat
There is constant temperature water pipe 41,37 degree of warm water is housed in the constant temperature water pipe 41, it, will be described after the front and back heat block 25 offsets
Cryopreservation tube 27 is wrapped in the thawing vat 29, will be filled between the umbilical cord in the cryopreservation tube 27 under the constant temperature water pipe 41 effect
Matter quick-thawing；
It is equipped with the lower holding tray 30 for being located at 26 downside of holding tray in the working chamber 11, is equipped in the lower holding tray 30 upper
Under perforative lower putting hole 31, the lower putting hole 31 is located at 18 downside of the putting hole and, institute concentric with the putting hole 18
It states and is equipped with annular chamber 55 in lower putting hole 31, rotated in 55 bottom wall of annular chamber and be equipped with swivel becket 32, outside the swivel becket 32
Disc is fixed with convex block 54, and the annular chamber 55 is connected to the lower putting hole 31, and 55 bottom wall of annular chamber is equipped with guiding
Slot 57, sliding is equipped with guide pad 58 in the guide groove 57, is connected between 57 inner wall of the guide pad 58 and the guide groove
Connecting spring 59,58 upper end of guide pad are fixed with compression bar 53, and described 53 one end of compression bar, which is fixed with, may extend to
Gripping block 56 in the lower putting hole 31, after the completion of defrosting, the holding tray 26 is driven under the cryopreservation tube 27 described in general who has surrendered
Cryopreservation tube 27 is inserted into the lower putting hole 31, and the swivel becket 32 rotates, and presses the compression bar 53 by the convex block 54
The gripping block 56 is extended in the lower putting hole 31 to clamp the cryopreservation tube 27, the holding tray 26, which rises, to be resetted,
To which the closing cap 17 be separated with the cryopreservation tube 27.
11 or so the wall of working chamber is symmetrically arranged with upper hopper chute 15, and sliding is equipped with top shoe 16, institute in the upper hopper chute 15
It states and is connected with upper connecting rod 40 between holding tray 26 and the top shoe 16,11 roof of working chamber is equipped with upper motor 12,
It has been connected by power screw shell 13 on the downside of the upper motor 12, threaded rod 14, institute are threaded in the screw shell 13
14 lower end of threaded rod is stated to be fixedly connected with 26 upper surface of holding tray.
11 bottom wall of the working chamber rotation is equipped with gear shaft 34, and the gear shaft 34 is fixedly connected with the swivel becket 32,
It is fixed with gear 33 on the gear shaft 34, be equipped with capable of sliding back and forth in the working chamber 11 and is engaged with the gear 33
Rack gear 35.
11 front and rear wall of working chamber, which symmetrically rotates, is equipped with lower threaded rod 43, and 11 rear wall of working chamber is fixed with lower electricity
Machine 45, the lower motor 45 are connected by power with the rear side lower threaded rod 43, pass through belt wheel between the lower threaded rod 43 of front and back
Mechanism 44 is sequentially connected, and the front and back heat block 25 is located remotely from each other end face and is fixed with mounting rack 42, the mounting rack 42 and institute
It states lower threaded rod 43 to be threadedly coupled, 25 left side of heat block is fixed with lower connecting rod 21, before 11 left wall of working chamber
After be symmetrically arranged with gliding groove 39, sliding is equipped with sliding block 38, the lower connecting rod 21 and the sliding block in the gliding groove 39
38 are fixedly connected.
11 left wall of working chamber is equipped with first sliding groove 46, and sliding is equipped with the first sliding block 48, institute in the first sliding groove 46
It states and is connected with reset spring 47 between 46 antetheca of the first sliding block 48 and the first sliding groove, 48 right side of the first sliding block is equipped with
Spring groove 49, sliding is equipped with first connecting rod 24, the first connecting rod 24 and 49 bottom of spring groove in the spring groove 49
Top pressure spring 50 is connected between wall, 24 right end of first connecting rod is fixed with intermediate plate 23, the intermediate plate 23 and institute
State and be fixedly connected with intermediate bar 36 between rack gear 35,24 right side of first connecting rod is fixed with driver plate 22, it is described on
In sliding slot 15 sliding be equipped with positioning plate 19,19 lower end surface of positioning plate be fixed with extend downward into the working chamber 11 and
The drive rod 20 to offset with 22 upper surface of driver plate is equipped with the accommodating groove 52 of upward opening in the intermediate plate 23, described
Sliding is equipped with ramp blocks 37 in accommodating groove 52, is connected with buffer spring between 52 bottom wall of the ramp blocks 37 and the accommodating groove
51。
The gripping block 56 is arc, and the gripping block 56 is rubber material.
There are four the putting hole 18 is set, there are four the lower putting hole 31 is set.
The sequence of the mechanical action of whole device:
1. four cryopreservation tubes 27 are placed in putting hole 18, closing cap 17 is located at 18 upper surface of putting hole；
2. the lower starting of motor 45 rotates the lower threaded rod 43 in front and back by the transmission of belt wheel mechanism 44, front and back mounting rack 42 is mutually leaned on
Closely, front and back heat block 25 is driven to offset, so that thawing vat 29 wraps up cryopreservation tube 27, rear side lower connecting rod 21 is moved at this time
To 37 front side of ramp blocks；
3. the umbilical cord mesenchymal stem cells fast melt frozen under the effect of constant temperature water pipe 41, in cryopreservation tube 27；
4. after reaching specified thawing time, the lower starting of motor 45 reversion makes heat block 25 be back to initial position, connected under rear side
Bar 21 drives intermediate plate 23 to move backward by ramp blocks 37；
5. intermediate plate 23 is moved by intermediate bar 36 with carry-over bar 35 backward, to rotate with moving gear 33, pass through gear shaft 34
Drive swivel becket 32 rotate, so that convex block 54 is no longer offseted with compression bar 53, connecting spring 59 effect under, by guide pad 58,
Compression bar 53 drives gripping block 56 to exit from lower putting hole 31；
6. the starting of motor 12 drives screw shell 13 to rotate on, and upper threaded rod 14 drives holding tray 26 to move downward, by cryopreservation tube
27 are inserted into lower putting hole 31, and when holding tray 26 drops to designated position, top shoe 16 pushes positioning plate 19, at this time upper motor
12 stop, and positioning plate 19 pushes driver plate 22 and intermediate plate 23 is driven to decline, ramp blocks 37 move to the downside of lower connecting rod 21 and with
The disengaging of rear side lower connecting rod 21 offsets, and is driven under 47 reset response of reset spring by the first sliding block 48, first connecting rod 24
Intermediate plate 23 is back to initial position, so that rack gear 35 be made to be back to initial position and rotate with moving gear 33, convex block 54 is pressed
Compression bar 53 drives gripping block 56 to protrude into lower putting hole 31 and clamps cryopreservation tube 27；
The starting of motor 12 reversion drives holding tray 26 to rise and resets, make closing cap 17 and cryopreservation tube 27 de- in uphill process on 7.
From to complete the unlatching of umbilical cord mesenchymal stem cells fast melt Yu cryopreservation tube 27.
The above embodiments merely illustrate the technical concept and features of the present invention, and its object is to allow be familiar with this field technology
Personage can understand the content of present invention and be implemented, and it is not intended to limit the scope of the present invention.It is all according to the present invention
Equivalent change or modification made by Spirit Essence, should be covered by the scope of protection of the present invention.
Claims (7)
1. a kind of culture device of pilot scale culture umbilical cord mesenchymal stem cells, including shell and be set in the shell and
To the working chamber of open front, it is characterised in that: be equipped with holding tray moving up and down in the working chamber, set in the holding tray
There is perforative putting hole up and down, cryopreservation tube is placed with the putting hole when use, be located at closing cap on the holding tray
Surface；
It is equipped with the heat block being located on the downside of the holding tray in the working chamber, is symmetrical arranged before and after the heat block, it is described to add
Heat block can move forward and backward, and thawing vat is equipped in the heat block, is fixed with constant temperature water pipe, the perseverance on the thawing vat inner wall
37 degree of warm water is housed in warm water tube, after the front and back heat block offsets, the cryopreservation tube is wrapped in the thawing vat
In, by the umbilical cord mesenchyma quick-thawing in the cryopreservation tube under constant temperature water pipe effect；
It is equipped with the lower holding tray being located on the downside of the holding tray in the working chamber, is equipped in the lower holding tray perforative up and down
Lower putting hole, the lower placement hole location is on the downside of the putting hole and concentric with the putting hole, is equipped in the lower putting hole
Annular chamber, rotation is equipped with swivel becket in the annular chamber bottom wall, and the swivel becket periphery is fixed with convex block, the annular chamber
It is connected to the lower putting hole, the annular chamber bottom wall is equipped with guide groove, and sliding is equipped with guide pad in the guide groove, described to lead
To connecting spring is connected between block and the guide groove inner wall, the guide pad upper end is fixed with compression bar, the press
Bar one end is fixed with the gripping block that may extend in the lower putting hole, and after the completion of defrosting, the holding tray drives the jelly
It deposits cryopreservation tube described in general who has surrendered under pipe to be inserted into the lower putting hole, the swivel becket rotation presses the top by the convex block
Compression bar extends to the gripping block in the lower putting hole to clamp the cryopreservation tube, and the holding tray, which rises, to be resetted, thus
The closing cap is separated with the cryopreservation tube.
2. a kind of culture device of pilot scale culture umbilical cord mesenchymal stem cells, feature exist according to claim 1
Be symmetrically arranged with upper hopper chute in: described working chamber or so wall, sliding is equipped with top shoe in the upper hopper chute, the holding tray with it is described
Upper connecting rod is connected between top shoe, the working chamber roof is equipped with upper motor, has been connected by power on the downside of the upper motor
Screw shell is threaded with threaded rod, the upper threaded rod lower end and the holding tray upper surface in the screw shell
It is fixedly connected.
3. a kind of culture device of pilot scale culture umbilical cord mesenchymal stem cells, feature according to claim 2 exist
In: the working chamber bottom wall rotation is equipped with gear shaft, and the gear shaft is fixedly connected with the swivel becket, solid on the gear shaft
Surely it is equipped with gear, rack gear that is capable of sliding back and forth and engaging with the gear is equipped in the working chamber.
4. a kind of culture device of pilot scale culture umbilical cord mesenchymal stem cells, feature according to claim 3 exist
In: the working chamber front and rear wall symmetrically rotates and is equipped with lower threaded rod, and the working chamber rear wall is fixed with lower motor, the lower electricity
Machine is connected by power with the rear side lower threaded rod, is sequentially connected between the lower threaded rod of front and back by belt wheel mechanism, front and back institute
It states heat block and is located remotely from each other end face and be fixed with mounting rack, the mounting rack is threadedly coupled with the lower threaded rod, the heating
Block left side is fixed with lower connecting rod, is symmetrically arranged with gliding groove before and after the working chamber left wall, slides and set in the gliding groove
There is sliding block, the lower connecting rod is fixedly connected with the sliding block.
5. a kind of culture device of pilot scale culture umbilical cord mesenchymal stem cells, feature according to claim 4 exist
In: the working chamber left wall is equipped with first sliding groove, and sliding is equipped with the first sliding block, first sliding block and institute in the first sliding groove
It states and is connected with reset spring between first sliding groove antetheca, first sliding block right side is equipped with spring groove, slides in the spring groove
It is dynamic to be equipped with first connecting rod, top pressure spring is connected between the first connecting rod and the spring groove bottom wall, described first connects
Extension bar right end is fixed with intermediate plate, and intermediate bar, first connection are fixedly connected between the intermediate plate and the rack gear
Bar right side is fixed with driver plate, and sliding is equipped with positioning plate in the upper hopper chute, the positioning plate lower face be fixed with to
Under the drive rod that extends in the working chamber and offset with the driver plate upper surface, be equipped with upward opening in the intermediate plate
Accommodating groove, sliding is equipped with ramp blocks in the accommodating groove, is connected with buffering between the ramp blocks and the storage groove bottom wall
Spring.
6. a kind of culture device of pilot scale culture umbilical cord mesenchymal stem cells, feature exist according to claim 1
In: the gripping block is arc, and the gripping block is rubber material.
7. a kind of culture device of pilot scale culture umbilical cord mesenchymal stem cells, feature exist according to claim 1
In: there are four the putting hole is set, there are four the lower putting hole is set.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910618105.6A CN110157599A (en) | 2019-07-10 | 2019-07-10 | A kind of culture device of pilot scale culture umbilical cord mesenchymal stem cells |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910618105.6A CN110157599A (en) | 2019-07-10 | 2019-07-10 | A kind of culture device of pilot scale culture umbilical cord mesenchymal stem cells |
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Cited By (6)
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| CN112322484A (en) * | 2020-10-31 | 2021-02-05 | 青岛领生生物科技有限公司 | Automatic thawing system for biological samples |
| CN112608846A (en) * | 2021-01-15 | 2021-04-06 | 吉林大学中日联谊医院 | Multifunctional cell culture device for improving premature ovarian failure stem cell exosomes |
| CN113446804A (en) * | 2020-03-26 | 2021-09-28 | 成都科莱斯液氮容器有限公司 | Liquid nitrogen bio-container is with square frame of depositing that freezes |
| CN113667602A (en) * | 2021-08-03 | 2021-11-19 | 广东省人民医院 | High-purity stem cell culture automation equipment |
| CN114703137A (en) * | 2021-12-31 | 2022-07-05 | 浙江金时代生物技术有限公司 | Method for differentiating umbilical cord mesenchymal stem cells into neural stem cells by utilizing cryopreserved umbilical cord mesenchymal stem cells |
| CN113667602B (en) * | 2021-08-03 | 2024-05-28 | 广东省人民医院 | High-purity stem cell culture automation equipment |
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