CN212952102U

CN212952102U - Cell culture bottle placing box

Info

Publication number: CN212952102U
Application number: CN201922443338.5U
Authority: CN
Inventors: 祝让飞
Original assignee: Hangzhou Mugu Technology Co ltd
Current assignee: Hangzhou Mugu Technology Co ltd
Priority date: 2019-12-30
Filing date: 2019-12-30
Publication date: 2021-04-13
Anticipated expiration: 2029-12-30

Abstract

The utility model discloses a cell culture bottle places box, comprises a box bod and a box cover, the lid is located the upside of box body, be equipped with the bottle plug of a plurality of joints on the upper end inside wall of lid, the upside of lid is equipped with rather than fixed connection's label plate, the last lateral wall of box body is equipped with places the hole the same with bottle plug quantity, be equipped with the cavity in the box body, the cavity with place the hole intercommunication, be equipped with the insulation can rather than fixed connection on the preceding lateral wall of box body, be equipped with a plurality of T venturi tubes rather than the intercommunication on the lateral wall that is close to the box body in the insulation can, the other end of T venturi tube passes the preceding lateral wall of box body and communicates with the cavity, the parallel is equipped with two and T venturi tube vertically push pedal and fixed plate on the horizontal direction in the insulation can. The utility model relates to a cell culture equipment technical field can continue to supply heat to the cell culture bottle under the condition that has a power failure, guarantees going on smoothly of cell culture experiment.

Description

Cell culture bottle placing box

Technical Field

The utility model relates to a cell culture equipment technical field especially relates to a box is placed to cell culture bottle.

Background

Cell culture refers to a method for simulating in-vivo environment (asepsis, proper temperature, pH value, certain nutrition condition, etc.) in vitro to enable the in-vivo environment to survive, grow, reproduce and maintain main structure and function, the commonly used cell culture device comprises a culture bottle and a culture plate, the cell culture needs proper temperature, some needs higher than room temperature, at the moment, heat needs to be supplied to cells, the commonly used heat supply mode comprises water bath heating and electric heating, the water bath heating has the function of controlling the temperature in a proper range, the heating is stable, the direct heating is not as violent and easy to control, the heating is uniform, the reaction needing strict temperature control is suitable, when the cells cultured in the cell culture bottle are heated by the water bath, the water is usually heated by a resistance wire to keep the constant temperature to carry out heat preservation treatment on the culture bottle, but the device cannot work under the condition of power failure, failing the cell culture assay.

To this end, we propose a cell culture flask housing box to solve the above problems.

SUMMERY OF THE UTILITY MODEL

The utility model aims at solving the problems existing in the prior art and providing a cell culture bottle placing box.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

a cell culture bottle placing box comprises a box body and a box cover, wherein the box cover is positioned on the upper side of the box body, a plurality of clamped bottle stoppers are arranged on the inner side wall of the upper end of the box cover, a label plate fixedly connected with the box cover is arranged on the upper side of the box cover, placing holes with the same number as the bottle stoppers are arranged on the upper side wall of the box body, a cavity is arranged in the box body and communicated with the placing holes, a heat preservation box fixedly connected with the box body is arranged on the front side wall of the box body, a plurality of T-shaped pipes communicated with the heat preservation box are arranged on the side wall, close to the box body, in the heat preservation box, the other end of each T-shaped pipe penetrates through the front side wall of the box body and is communicated with the cavity, two push plates and a fixed plate which are vertical to the T-shaped pipes in the horizontal direction are arranged, the front side of the heat preservation box is parallelly inserted with a threaded rod and a rotating rod which are rotationally connected with the threaded rod, one end of the threaded rod, which is positioned in the heat preservation box, is sleeved with a first sleeve which is in threaded connection with the threaded rod, the other end of the first sleeve penetrates through the fixed plate and is rotationally connected with one side of the push plate, one end of the threaded rod, which is far away from the first sleeve, is provided with a gear which is fixedly connected with the threaded rod, the other end of the rotating rod is sleeved with a second sleeve which is rotationally connected with the rotating rod, one end of the second sleeve is fixedly connected with the inner side wall of the heat preservation box, one end of the rotating rod, which is positioned in the second sleeve, is sleeved with a clockwork spring, one end of the rotating rod, which is far away from the second sleeve, is provided with a rotating disc which is fixedly connected with the, and a heat insulation material is arranged outside the water tank.

Preferably, handles fixedly connected with the box cover are symmetrically arranged at two ends of the box cover, and anti-skid rubber sleeves are sleeved on the handles.

Preferably, a rotating groove is formed in the front side wall of the heat preservation box, a limiting block fixedly connected with the threaded rod is sleeved on the threaded rod, and the limiting block is rotatably connected with the rotating groove.

Preferably, a blocking plug in sliding connection with the T-shaped pipe is inserted into one end, away from the heat preservation box, of the T-shaped pipe, springs fixedly connected with one side of the blocking plug are symmetrically arranged on one side of the blocking plug, and the other end of each spring is fixedly connected with the inner wall of the T-shaped pipe.

Preferably, the stopper is sleeved with a sealing ring, and the outer diameter of the sealing ring is larger than the inner diameter of the T-shaped pipe.

Compared with the prior art, the beneficial effects of the utility model are that:

1. the cell culture bottle placing box is arranged, the water bath device is additionally arranged in the placing box, hot water with the temperature required by cell culture is filled in the water tank with the heat-insulating material, meanwhile, the hot water in the water tank is injected into the placing box through the water feeding device, meanwhile, the spring is tightened to stress the hot water, the elastic force after the spring is loosened drives the rotating disc connected with the rotating rod to rotate, the rotating disc is provided with straight teeth meshed with the gear to drive the threaded rod connected with the gear to rotate, the threaded rod is in threaded connection with the first sleeve, the threaded rod rotates to drive the first sleeve to move forwards, the push plate is pushed to move in the heat-insulating box, the space on the right side of the push plate in the heat-insulating box is reduced, the pressure of the hot water is increased and is larger than the elastic force of the spring, so that the blocking plug is drawn out of the T-shaped pipe, the hot, the whole process is driven by a mechanical structure, and water is not heated by electric power, so that the problem that the cell culture bottle cannot be heated when power is cut off is solved, and the test is ensured to be carried out;

2. the rotating disc is provided with partial straight teeth, when the straight teeth on the rotating disc are contacted with the gear again for meshing transmission, the actions are repeated to inject hot water into the box body, so that intermittent heat supply is realized, and the water demand of the whole heat supply process is reduced while the temperature of the cell culture bottle in the box body is ensured.

Drawings

FIG. 1 is a schematic structural view of a cell culture bottle storage case according to the present invention;

FIG. 2 is a schematic view of a structure of a lid of a cell culture bottle placing box according to the present invention;

FIG. 3 is a sectional view of an incubator of the cell culture flask holding box according to the present invention;

FIG. 4 is a cross-sectional view of the T-shaped tube of the cell culture bottle placing box of the present invention.

In the figure: the bottle stopper comprises a box body 1, a box cover 2, a bottle stopper 3, a lifting handle 4, a label plate 5, a placing hole 6, an insulation box 7, a water tank 8, a pushing plate 9, a fixing plate 10, a first sleeve 11, a threaded rod 12, a gear 13, a second sleeve 14, a rotating rod 15, a rotating disc 16, a clockwork spring 17, a T-shaped pipe 18, a spring 19 and a stopper plug 20.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments.

In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.

Referring to fig. 1-4, a cell culture bottle placing box comprises a box body 1 and a box cover 2, wherein the box cover 2 is arranged at the upper side of the box body 1, a plurality of clamped bottle stoppers 3 are arranged on the inner side wall at the upper end of the box cover 2, the bottle stoppers 3 are clamped with the box cover to facilitate taking down and cleaning the bottle stoppers 3, handles 4 fixedly connected with the box cover 2 are symmetrically arranged at two ends of the box cover 2, anti-slip rubber sleeves are sleeved on the handles 4, anti-slip handles 4 are additionally arranged to facilitate taking down the box cover 2 from the box body 1, a label plate 5 fixedly connected with the box cover 2 is arranged at the upper side of the box cover 2, placing holes 6 with the same number as the bottle stoppers 3 are arranged on the upper side wall of the box body 1, a cavity,

the front side wall of the box body 1 is provided with an insulation box 7 fixedly connected with the insulation box, the side wall of the insulation box 7 close to the box body 1 is provided with a plurality of T-shaped pipes 18 communicated with the insulation box, the other ends of the T-shaped pipes 18 penetrate through the front side wall of the box body 1 and are communicated with the cavity, one end of the T-shaped pipe 18 far away from the insulation box 7 is inserted with a blocking plug 20 in sliding connection with the insulation box, one side of the blocking plug 20 is symmetrically provided with a spring 19 fixedly connected with the blocking plug, the other end of the spring 19 is fixedly connected with the inner wall of the T-shaped pipe 18, the spring 19 is additionally arranged to ensure that the blocking plug 20 can be pulled out of the T-shaped pipe 18 when the water pressure in the insulation box 7 is larger than the elastic force of the spring 19, when the water pressure is smaller than the elastic force of the spring 19, the blocking plug 20 and the T-shaped pipe, after the stop plug 20 is reset, the sealing performance between the stop plug 20 and the T-shaped pipe 18 can be ensured,

the heat preservation box 7 is internally provided with two push plates 9 and a fixed plate 10 which are vertical to a T-shaped pipe 18 in the horizontal direction in parallel, the push plates 9 are positioned at the rear side of the fixed plate 10, the push plates 9 are connected with the inner side wall of the heat preservation box 7 in a sliding way, the fixed plate 10 is fixedly connected with the inner side wall of the heat preservation box 7, a threaded rod 12 and a rotating rod 15 which are connected with the front side of the heat preservation box 7 in a rotating way are inserted in parallel, one end of the threaded rod 12 positioned in the heat preservation box 7 is sleeved with a first sleeve 11 which is connected with the threaded rod in a threaded way, the other end of the first sleeve 11 penetrates through the fixed plate 10 and is connected with one side of the push plates 9 in a rotating way, one end of the threaded rod 12 far away from the first sleeve 11 is provided with a gear 13 which is fixedly connected with the threaded rod, the degree of freedom of the threaded rod 12 in the horizontal direction is limited, the threaded rod 12 is prevented from rotating and moving, the first sleeve 11 and the threaded rod 12 only rotate and do not move,

the other pot head of dwang 15 is equipped with rather than the second sleeve 14 that rotates the connection, the one end of second sleeve 14 and insulation can 7's inside wall fixed connection, dwang 15 is located one of second sleeve 14 and serves the cover and be equipped with clockwork spring 17, dwang 15 keeps away from second sleeve 14's one end and is equipped with rather than fixed connection's rolling disc 16, the lateral wall top portion of rolling disc 16 is equipped with the straight-tooth of being connected with gear 13 meshing, insulation can 7's upper end is equipped with rather than the water tank 8 that communicates, water tank 8's mouth of a river is located the rear side of push pedal 9, water tank 8 is equipped with insulation material outward, insulation material is established with on water tank 8, guarantee the temperature.

In the utility model, firstly, the culture bottle with cells is placed in the placing hole 6 on the box body 1, then the box cover 2 is covered on the box body 1, so that the bottle cork 3 blocks the opening of the culture bottle, 37 ℃ hot water is injected into the water tank 8, at the moment, the hot water enters the position of the heat preservation box 7 on the right side of the push plate 9 through the water gap, then the rotating rod 15 is rotated, the spiral spring 17 is driven to rotate through the rotation of the rotating rod 15, the spiral spring 17 is tightened, after the spiral spring 17 is loosened, power is generated by utilizing the elastic force of the spiral spring to gradually loosen, the rotating disc 16 connected with the rotating rod 15 is driven to rotate, when the straight tooth on the rotating disc 16 is meshed with the gear 13, the threaded rod 12 connected with the gear 13 is driven to rotate, because the threaded rod 12 is in threaded connection with the first sleeve 11, and the threaded rod 12 only rotates on the heat preservation box 7, the threaded, and then the push plate 9 is pushed to move towards the direction of the T-shaped pipe 18, so that the space on the right side of the push plate 9 in the heat preservation box 7 is reduced, the pressure of hot water is increased and is larger than the elastic force of the spring 19, so that the stop plug 20 is pulled out of the T-shaped pipe 18, the hot water flows into the cavity in the box body 1 through the T-shaped pipe 18, the cell culture bottle is heated and preserved in a water bath, when the straight teeth on the rotating disc 16 are disengaged from the gear 13, the gear 13 stops rotating, the threaded rod 12 connected with the gear 13 stops rotating, the first sleeve 11 in threaded connection with the threaded rod 12 stops rotating, the push plate 9 stops moving, the space on the right side of the push plate 9 in the heat preservation box 7 cannot be reduced, at the moment, under the elastic force of the spring 19, the stop plug 20 moves back to block the T-shaped pipe 18, the hot water injection into the cavity in the box body 1 is, when the straight teeth on the rotating disc 16 are contacted with the gear 13 again for meshing transmission, the above actions are repeated to inject hot water into the box body 1, so that the temperature of the cell culture bottle in the box body 1 is ensured.

The above, only be the concrete implementation of the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is in the technical scope of the present invention, according to the technical solution of the present invention and the utility model, the concept of which is equivalent to replace or change, should be covered within the protection scope of the present invention.

Claims

1. The utility model provides a box is placed to cell culture bottle, includes box body (1) and lid (2), its characterized in that, lid (2) are located the upside of box body (1), be equipped with bottle plug (3) of a plurality of joints on the upper end inside wall of lid (2), the upside of lid (2) is equipped with rather than fixed connection's label plate (5), the last lateral wall of box body (1) is equipped with places hole (6) the same with bottle plug (3) quantity, be equipped with the cavity in box body (1), the cavity with place hole (6) intercommunication, be equipped with on the preceding lateral wall of box body (1) rather than fixed connection's insulation can (7), be equipped with a plurality ofly rather than the T venturi tube (18) of intercommunication on the lateral wall that is close to box body (1) in insulation can (7), the other end of T venturi tube (18) passes the preceding lateral wall of box body (1) and communicates with the cavity, the parallel is equipped with two in insulation can (7) and T venturi tube (18) the vertical (9) And a fixed plate (10), the push plate (9) is positioned at the rear side of the fixed plate (10), the push plate (9) is connected with the inner side wall of the heat preservation box (7) in a sliding manner, the fixed plate (10) is fixedly connected with the inner side wall of the heat preservation box (7), a threaded rod (12) and a rotating rod (15) which are connected with the heat preservation box in a rotating manner are inserted in parallel at the front side of the heat preservation box (7), a first sleeve (11) which is connected with the threaded rod in a threaded manner is sleeved at one end of the threaded rod (12) positioned in the heat preservation box (7), the other end of the first sleeve (11) penetrates through the fixed plate (10) and is connected with one side of the push plate (9) in a rotating manner, a gear (13) which is fixedly connected with the threaded rod is arranged at one end of the threaded rod (12) far away from the first sleeve (11), a second sleeve (14) which is connected with the rotating manner, dwang (15) are located one of second sleeve (14) and serve the cover and be equipped with clockwork spring (17), the one end that second sleeve (14) was kept away from in dwang (15) is equipped with rolling disc (16) rather than fixed connection, the lateral wall top portion of rolling disc (16) is equipped with the straight-tooth of being connected with gear (13) meshing, the upper end of insulation can (7) is equipped with water tank (8) rather than the intercommunication, the mouth of a river of water tank (8) is located the rear side of push pedal (9), water tank (8) are equipped with insulation material outward.

2. The cell culture bottle placing box according to claim 1, wherein handles (4) fixedly connected with the box cover (2) are symmetrically arranged at two ends of the box cover, and anti-skid rubber sleeves are sleeved on the handles (4).

3. The cell culture bottle placing box according to claim 1, wherein a rotating groove is formed on the front side wall of the heat preservation box (7), a limiting block fixedly connected with the threaded rod (12) is sleeved on the threaded rod, and the limiting block is rotatably connected with the rotating groove.

4. The cell culture bottle placing box according to claim 1, wherein a stop plug (20) is inserted into one end of the T-shaped pipe (18) far away from the heat preservation box (7) and is connected with the end in a sliding manner, a spring (19) is symmetrically arranged on one side of the stop plug (20) and is fixedly connected with the stop plug, and the other end of the spring (19) is fixedly connected with the inner wall of the T-shaped pipe (18).

5. The cell culture bottle placing box according to claim 4, wherein the stopper (20) is sleeved with a sealing ring, and the outer diameter of the sealing ring is larger than the inner diameter of the T-shaped pipe (18).