CN114717113A - Cell culture bag for constant-current fluid infusion - Google Patents
Cell culture bag for constant-current fluid infusion Download PDFInfo
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- CN114717113A CN114717113A CN202210375342.6A CN202210375342A CN114717113A CN 114717113 A CN114717113 A CN 114717113A CN 202210375342 A CN202210375342 A CN 202210375342A CN 114717113 A CN114717113 A CN 114717113A
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
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- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
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Abstract
A cell culture bag for constant-current fluid infusion comprises a supporting plate, wherein one side of the supporting plate is connected with a sucker, the upper part of the supporting plate is provided with a first through hole, one side of the supporting plate is connected with a supporting rod, one end of the supporting rod is connected with an injection body, the upper end of the injection body is connected with a clamping mechanism, the interior of the injection body is fixedly connected with a rotating mechanism, and the lower end face of the rotating mechanism is connected with a pushing mechanism; the lower end face of the injection body is connected with a cell culture bag, a sucker on the supporting plate is firstly selected to be adsorbed on a wall body or hung on a hook through a first through hole, the two fixing modes can be used simultaneously, and after the sucker and the first through hole are fixed, the cell culture bag is placed in a supporting groove in advance to avoid dropping of the cell culture bag.
Description
Technical Field
The invention relates to the technical field of biological cell culture, in particular to a cell culture bag for constant-current fluid infusion.
Background
In the fields of immune cell therapy of tumors and scientific researches of biology, immunity, medicine and the like, a cell culture technology is often used and is also the basis of almost all cell biology experiments. During the cell culture period, the cells can be continuously grown and reach a certain number by passage and fluid replacement. To achieve higher numbers of cells, larger cell culture bags are often used. When a cell culture bag is used for culturing cells for passage or fluid replacement, a syringe of a 50ml syringe is generally used as a funnel and connected with the cell culture bag, and then culture fluid is poured into the syringe, and the culture fluid flows into the cell culture bag through the syringe to complete the passage or fluid replacement process.
Because the current injection body is only suitable for the culture solution bottle to install, but the culture solution bottle of installation can only be with suitable model, it is too single, can not install the fluid infusion by a plurality of different models, and also can not install bagged culture solution, cause the suitability that reduces the injection body, reduce work efficiency, the culture solution in the injection body can solidify along with the change of time, cause the obstructed phenomenon of culture solution runner or flow slowly, and the culture solution is in the pipe guide flow, the pipe guide can appear crooked great sometimes, cause the culture solution to flow slowly or jam phenomenon.
In order to solve the problems, the invention provides a cell culture bag for constant-current fluid infusion.
Disclosure of Invention
(1) Technical problem to be solved
The invention aims to overcome the problems in the prior art, adapt to the practical needs, and provide a cell culture bag for constant-current fluid infusion so as to solve the technical problems.
(2) Technical scheme
In order to realize the purpose of the invention, the technical scheme adopted by the invention is as follows: a cell culture bag for constant-current fluid infusion comprises a supporting plate, wherein one side of the supporting plate is connected with a sucker, the upper part of the supporting plate is provided with a first through hole, one side of the supporting plate is connected with a supporting rod, one end of the supporting rod is connected with an injection body, the upper end of the injection body is connected with a clamping mechanism, the interior of the injection body is fixedly connected with a rotating mechanism, and the lower end face of the rotating mechanism is connected with a pushing mechanism; the lower end face of the injection body is connected with a cell culture bag.
Preferentially, the injection body comprises a first accommodating shell, one side of the outer surface of the first accommodating shell is connected to one end of the supporting rod, the upper end face of the accommodating shell is connected with the access port, the lower end face of the access port is connected with the receiving port, the lower end face of the receiving port is connected with the second accommodating shell, a filter screen is installed at the joint of the receiving port and the second accommodating shell, the middle of the lower end face of the second accommodating shell is connected with a connecting plate, the lower end face of the outer surface of the second accommodating shell is connected to the inner surface of the lower end of the first accommodating shell, two sides of the connecting plate are connected to two sides of the inner surface of the second accommodating shell, the lower end face of the second accommodating shell is connected with the discharge port, the discharge port is right opposite to the lower end face of the second accommodating shell, the discharge port is right opposite to the lower end face of the connecting plate, and the discharge port is connected with a guide pipe.
Preferentially, fixture includes the supporting ring board, end connection is in the first surface upper end that holds the shell under this supporting ring board, and supporting ring inboard surface equipartition has first spring body around encircling, this first spring one end is connected with first grip block, this first grip block up end is connected with the fixed plate, the slide groove has been seted up at this fixed plate up end middle part, this slide inslot portion sliding connection has the sliding part, this sliding part up end is connected with the second grip block, this second grip block one side surface connection has the grip block, and the second grip block passes through the elastic cord and connects a plurality of second grip blocks, and grip block one side surface butt has the culture solution bottle.
Preferably, the rotating mechanism comprises a flow rod, the upper end of the flow rod is connected to the inner upper part of the second accommodating shell, the lower part of the flow rod is connected with a bottom shell, a motor is connected to the bottom shell, the output end of the motor is connected with a rotating shaft, one end of the rotating shaft is connected to the lower end face of the flow rod, the flow rod is in rotating connection and matching with the bottom shell, the upper end face of the flow rod is provided with a tip, the outer surface of the upper end of the flow rod is connected with a first rotating plate, second through holes are uniformly distributed in the first rotating plate, the first rotating plate is in rotating connection with a first rotating groove, the first rotating groove is formed in the inner surface of the upper end of the second accommodating shell, the outer surface of the lower end of the flow rod is connected with a second rotating plate, a third through hole is formed in the second rotating plate, the outer surface of the second rotating plate is connected with a protection plate, and the outer surface of one side of the protection plate is abutted against the inner surface of the second accommodating shell, the outer surface of the protection plate is sleeved with a transmission circular plate, the transmission circular plate is connected with a second rotation groove in a rotating mode, the second rotation groove is formed in the middle of the second containing shell, the outer surface of the transmission circular plate is connected with a rotating disc, the rotating disc is connected with a third rotation groove in a rotating mode, and the third rotation groove is formed in the lower end of the first containing shell.
Preferentially, pushing mechanism includes the transfer line, and this transfer line one end is connected at the lower terminal surface of rolling disc, and the transfer line lower terminal surface is connected with the rotation portion, and the terminal surface is connected with the ball under this rotation portion, and this ball surface butt has the catch bar, and the terminal surface is connected with the slurcam under this catch bar, and the terminal surface is connected with first fixed plate under this slurcam, and is connected with the heating pipe under the slurcam in the middle part of the terminal surface, and the slurcam runs through on the surface of stand pipe with the heating pipe, the terminal surface is connected with the second spring body under the first fixed plate, and this second spring body lower extreme is connected with the second fixed plate, and the lower extreme surface at the stand pipe is fixed to this second fixed plate.
Preferably, the cell culture bag is connected to a lower end surface of the guide tube, the support plate is connected to a lower end surface of the support plate, the support groove is V-shaped, and an outer surface of the cell culture bag abuts against an inner surface of the support groove.
Preferably, the plurality of push rods are arranged in a low-to-high circumference around the upper end surface of the push plate, and the upper end surface of the second fixing plate is disposed opposite to the lower end surface of the push plate.
(3) Has the advantages that:
A. firstly, selecting a sucker on a supporting plate to be adsorbed on a wall body or hanging the sucker on a hook by using a first through hole, and simultaneously using the two fixing modes, wherein after the sucker and the first through hole are fixed, the cell culture bag is placed in a supporting groove in advance to prevent the cell culture bag from falling;
B. then, the culture solution bottle is placed on the outer surface of the clamping block on the clamping mechanism, the culture solution bottle is vertically clamped by a person downwards, the outer surface of the culture solution bottle slides on the outer surfaces of the clamping blocks, the clamping blocks can pass through the volume of the culture solution bottle and the clamping force given by the person, and then the second clamping plates slide in the slide channel groove by using the sliding parts through the elasticity of the elastic band, the volume of the culture solution bottle can be outwards expanded and wrapped and clamped, the culture solution bottle continues to vertically downwards and can be abutted against the outer surfaces of the first clamping plates, the first spring bodies on the first clamping plates can stretch and retract to clamp the outer surface of the culture solution bottle until the culture solution bottle is abutted against the access port, so that the safe fixation of the culture solution bottle is completed, if the culture solution bag is a cylindrical shape, two sides of the culture solution bag can be rolled up by an operator, the nutrient solution bottle is placed on the outer surface of the clamping block, and the working process of placing the nutrient solution bottle is utilized, so that nutrient solution bottles of different types can be installed and replenished with the nutrient solution, the applicability of an injection body is improved, and the working efficiency is improved;
C. after the culture solution bottle is placed, the culture solution flows into the access port from the bottle mouth and flows into the access port through the guide of the access port, and then flows into the access port, in the process of the access port, the culture solution can be filtered through the filter screen and flows into the second accommodating shell, the culture solution is just opposite to the tip on the contact rotating mechanism, the tip can enable the culture solution to vertically flow downwards along the flowing rod, the motor starts to start and drives the rotating shaft to enable the flowing rod to rotate, the first rotating plate can rotate in the first rotating groove, the culture solution can rapidly flow under the rotation of the flowing rod and can be in contact with the outer surfaces of the first rotating plate and the second through hole to be stirred and circulated, the culture solution is prevented from being solidified, after the culture solution is stirred and circulated, the culture solution can flow to the outer surface of the second rotating plate along with the rotation of the flowing rod, and the culture solution is stirred and circulated again by the rotation of the second rotating plate and the third through hole, the second rotating plate can drive the protection plate to abut against the inner surface of the second containing shell, so that the culture solution is prevented from flowing out of the second rotating groove, the culture solution can continuously keep a liquid state through stirring and circulation of the second rotating plate and the third through hole, the culture solution can flow downwards along with the outer surface of the bottom shell after passing through the third through hole in the second rotating plate until flowing into the discharge port, and the discharge port is guided into the guide pipe, so that the culture solution in the injection body is prevented from being solidified due to time change, the phenomenon that a culture solution flow channel is blocked or the culture solution flows slowly is avoided, the flowability of the culture solution is improved, and the solidification of the culture solution is prevented;
D. when the rotating mechanism is started, the pushing mechanism can rotate along with the rotating mechanism, the transmission circular plate is driven to rotate through the protection plate, the transmission circular plate is driven to rotate in the second rotating groove, the transmission circular plate drives the rotating disc, the rotating disc is driven to rotate in the third rotating groove, the lower end of the outer side of the circle of the rotating disc can transmit a transmission rod on the pushing mechanism, the transmission rod is driven by the rotating disc to enable the round balls on the lower end face of the rotating part to be abutted against the push rods, the push rods are arranged in a low-to-high circumference, each time the round balls contact one push rod, the second spring body between the first fixing plate and the second fixing plate can be pressed, the second spring body can stretch downwards, the push plates on the lower end faces of the push rods can slide downwards together with the second spring body at the same time when the second spring body is pressed, and the push plates drive the heating pipe to slide on the outer surface of the guide pipe, this heating pipe not only can give the culture solution heating, still prevents the stand pipe bending, and the circulation of heating pipe accessible catch bar slides back and forth, and the culture solution in this stand pipe is along with the stable and quick flow of heating, lets in the smooth entering cell culture bag of culture solution, is favorable to avoiding the direction to appear crooked great, improves the culture solution mobility, lets the culture solution avoid solidifying and blocking phenomenon through heating methods.
Drawings
FIG. 1 is a schematic perspective view of a cell culture bag for constant flow fluid infusion according to the present invention;
FIG. 2 is a partially enlarged view of an injection body of a cell culture bag for constant flow fluid infusion according to the present invention;
FIG. 3 is a partially enlarged view of a clamping mechanism of a cell culture bag for constant flow fluid infusion according to the present invention;
FIG. 4 is a partially enlarged view of a rotating mechanism of a cell culture bag for constant flow fluid infusion according to the present invention;
FIG. 5 is a schematic diagram of the pushing mechanism of the cell culture bag for constant flow fluid infusion according to the present invention;
FIG. 6 is a schematic diagram of a cell culture bag for a constant flow fluid infusion cell culture bag according to the present invention.
The reference numbers are as follows:
1-support plate, 11-suction cup, 12-first through hole, 13-support bar, 14-support groove, 2-injection body, 21-first containing case, 221-third rotating groove, 22-inlet, 23-inlet, 24-second containing case, 241-first rotating groove, 242-second rotating groove, 25-filter screen, 26-connecting plate, 27-outlet, 28-guide tube, 3-clamping mechanism, 31-support ring plate, 32-first spring body, 33-first clamping plate, 34-fixing plate, 35-slide groove, 36-slide part, 37-second clamping plate, 38-clamping block, 381-elastic band, 39-culture solution bottle, 4-rotating mechanism, 41-flow bar, 411-bottom shell, 412-motor, 42-rotating shaft, 43-tip, 431-first rotating plate, 432-second through hole, 44-second rotating plate, 441-third through hole, 45-protection plate, 46-transmission circular plate, 47-rotating disc, 5-pushing mechanism, 51-transmission rod, 52-rotating part, 53-round ball, 54-pushing rod, 55-pushing plate, 56-first fixing plate, 57-heating pipe, 58-second spring body, 59-second fixing plate and 6-cell culture bag.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
The invention will be further illustrated with reference to the following figures 1-6 and examples:
in this embodiment, as shown in fig. 1-6, a cell culture bag for constant-current fluid infusion includes a support plate 1, a suction cup 11 is connected to one side of the support plate 1, a first through hole 12 is formed in an upper portion of the support plate 1, a support rod 13 is connected to one side of the support plate 1, an injection body 2 is connected to one end of the support rod 13, a clamping mechanism 3 is connected to an upper end of the injection body 2, a rotating mechanism 4 is fixedly connected to an inside of the injection body 2, and a pushing mechanism 5 is connected to a lower end surface of the rotating mechanism 4; the lower end surface of the injection body 2 is connected with a cell culture bag 6. Firstly, the suction cup 11 on the support plate 1 is selected to be adsorbed on the wall body or hung on the hook by the first through hole 12, and the two fixing modes can also be used simultaneously.
In this embodiment, the injection body 2 includes a first housing case 21, one side of the outer surface of the first housing case 21 is connected to one end of the support rod 13, and the upper end surface of the housing case 21 is connected to the inlet 22, the lower end surface of the inlet 22 is connected with an inlet 23, the lower end surface of the inlet 23 is connected with a second containing shell 24, a filter screen 25 is arranged at the joint of the receiving opening 23 and the second containing shell 24, a connecting plate 26 is connected with the middle part of the lower end surface of the second containing shell 24, and the lower end of the outer surface of the second receiving case 24 is coupled to the inner surface of the lower end of the first receiving case 21, the connecting plate 26 is connected at both sides to the inner surface of the second receiving case 24, the lower end surface of the second receiving case 24 is connected to the discharge port 27, the discharge inlet 27 is aligned with the lower end port of the second accommodation case 24, and the discharge inlet 27 is aligned with the lower end face of the connection plate 26, and a guide pipe 28 is connected to the lower end face of the discharge inlet 27. After placing culture solution bottle 39, the culture solution flows out of the bottle mouth to access port 22, flows into receiving port 23 through the guide flow of access port 22, and then flows into receiving port 23, and the culture solution can be filtered through filter screen 25 and flows into second containing shell 24, and the culture solution can flow downwards along with flow rod 41 and the outer surface of bottom shell 411 until flowing into discharging port 27, and the discharging port 27 is guided into guide pipe 28, so that impurities can be filtered, and the flow rate of the culture solution can be improved.
In this embodiment, the clamping mechanism 3 includes a supporting ring plate 31, the lower end surface of the supporting ring plate 31 is connected to the upper end of the outer surface of the first accommodating case 21, the inner surface of the supporting ring plate 31 is uniformly surrounded by a first spring body 32, one end of the first spring body 32 is connected to a first clamping plate 33, the upper end surface of the first clamping plate 33 is connected to a fixing plate 34, a chute 35 is formed in the middle of the upper end surface of the fixing plate 34, a sliding portion 36 is slidably connected to the inside of the chute 35, the upper end surface of the sliding portion 36 is connected to a second clamping plate 37, a clamping block 38 is connected to the outer surface of one side of the second clamping plate 37, the second clamping plate 37 is connected to a plurality of second clamping plates 37 through an elastic band 381, and a culture solution bottle 39 is abutted to the outer surface of one side of the clamping block 38. Then, the culture solution bottle 39 is placed on the outer surface of the holding block 38 on the holding mechanism 3, the culture solution bottle 39 is vertically held by a person downward, the outer surface of the culture solution bottle 39 is slid on the outer surface of the holding blocks 38, the holding blocks 38 are held by the volume of the culture solution bottle 39 and the holding force of the person, the second holding plates 37 are slid inside the slide groove 35 by the sliding part 36 due to the elasticity of the elastic band 381, the volume of the culture solution bottle 39 is expanded outward and held, the culture solution bottle 39 is continued to be vertically downward and is abutted against the outer surface of the first holding plates 33, the first spring bodies 32 on the first holding plates 33 are extended and contracted to hold the outer surface of the culture solution bottle 39 until the culture solution bottle 39 is abutted against the access port 22, thereby completing the safe fixation of the culture solution bottle 39, and the working process of placing the culture solution bottle 39 is utilized, the device is beneficial to installing and supplementing liquid for nutrient bottles of different types, improves the applicability of the injection body and improves the working efficiency.
In this embodiment, the rotating mechanism 4 includes a flow rod 41, an upper end of the flow rod 41 is connected to an inner upper portion of the second accommodating case 24, a bottom case 411 is connected to a lower portion of the flow rod 41, a motor 412 is connected to the bottom case 411, an output end of the motor 412 is connected to a rotating shaft 42, one end of the rotating shaft 42 is connected to a lower end surface of the flow rod 41, the flow rod 41 is rotatably connected and matched with the bottom case 411, a tip 43 is formed on an upper end surface of the flow rod 41, a first rotating plate 431 is connected to an outer surface of an upper end of the flow rod 41, second through holes 432 are uniformly formed in the first rotating plate 431, a first rotating groove 241 is rotatably connected to the first rotating plate 431, the first rotating groove 241 is formed in an inner surface of an upper end of the second accommodating case 24, a second rotating plate 44 is connected to an outer surface of a lower end of the flow rod 41, a third through hole 441 is formed in the second rotating plate 44, and a shielding plate 45 is connected to an outer surface of the second rotating plate 44, the outer surface of one side of the protection plate 45 abuts against the inner surface of the second accommodating case 24, a transmission circular plate 46 is sleeved on the outer surface of the protection plate 45, the transmission circular plate 46 is rotatably connected with a second rotating groove 242, the second rotating groove 242 is arranged in the middle of the second accommodating case 24, the outer surface of the transmission circular plate 46 is connected with a rotating disc 47, the rotating disc 47 is rotatably connected with a third rotating groove 211, and the third rotating groove 211 is arranged at the lower end of the first accommodating case 21. The culture solution is filtered by the filter screen 25 and flows into the second containing shell 24, and the culture solution is opposite to the top 43 on the contact rotating mechanism 4, the top 43 allows the culture solution to flow vertically downwards along the flow rod 41, at this time, the motor 412 starts to be started, and drives the rotating shaft 42 to rotate the flow rod 41, the first rotating plate 431 is rotated in the first rotating groove 241, and the culture solution is rapidly flowed by the rotation of the flow rod 41, and is contacted with the outer surfaces of the first rotating plate 431 and the second through hole 432 for stirring and flowing, so as to prevent the culture solution from solidifying, and after the culture solution is further circulated by stirring, the culture solution flows to the outer surface of the second rotating plate 44 along with the rotation of the flow rod 41, the culture solution is further stirred and circulated by the rotation of the second rotating plate 44 and the third through hole 441, and the second rotating plate 44 drives the protection plate 45 to abut against the inner surface of the second containing shell 24, the culture solution is prevented from flowing out of the second rotating tub 242, and the culture solution is continuously maintained in a liquid state by the agitation and circulation of the second rotating plate 44 and the third through-hole 441, after passing through the third through-hole 441 of the second rotating plate 44, the culture solution flows downward along the outer surface of the bottom case 411 until it flows into the discharge port 27, the discharge port 27 is guided into the guide tube 28, and when the rotation mechanism 4 is activated, the pushing mechanism 5 rotates along with the rotation, and the protection plate 45 drives the transmission circular plate 46 to rotate, so that the transmission circular plate 46 rotates in the second rotation groove 242, and the transmission circular plate 46 drives the rotating disc 47 to make the rotating disc 47 rotate in the third rotating groove 211, which is beneficial to preventing the culture solution in the injection body from solidifying due to time change, avoiding the phenomenon of obstructed culture solution flow channels or slow flowing, improving the fluidity of the culture solution and preventing the culture solution from solidifying.
In this embodiment, the pushing mechanism 5 includes a transmission rod 51, one end of the transmission rod 51 is connected to the lower end surface of the rotating disc 47, the lower end surface of the transmission rod 51 is connected to a rotating portion 52, the lower end surface of the rotating portion 52 is connected to a round ball 53, the outer surface of the round ball 53 is abutted to a pushing rod 54, the lower end surface of the pushing rod 54 is connected to a pushing plate 55, the lower end surface of the pushing plate 55 is connected to a first fixing plate 56, the middle portion of the lower end surface of the pushing plate 55 is connected to a heating pipe 57, an electric heating wire is wound around the inside of the heating pipe 57, the pushing plate 55 and the heating pipe 57 penetrate through the outer surface of the guide tube 28, the lower end surface of the first fixing plate 56 is connected to a second spring body 58, the lower end of the second spring body 58 is connected to a second fixing plate 59, and the second fixing plate 59 is fixed to the outer surface of the lower end of the guide tube 28. And the lower end of the round outer side of the rotating disc 47 will be transmitted to the transmission rod 51 on the pushing mechanism 5, so that the transmission rod 51 will make the round ball 53 on the lower end surface of the rotating part 52 abut against the pushing rod 54 under the rotation driving of the rotating disc 47, the pushing rod 54 is arranged in a circle with a height from low to high, and each time the round ball 53 contacts one pushing rod 54, the second spring 58 between the first fixing plate 56 and the second fixing plate 59 will be pressed, the second spring 58 will be stretched downwards, and at the same time of pressing the second spring 58, the pushing plate 55 on the lower end surface of the pushing rod 54 will slide downwards with the second spring 58, and the pushing plate 55 will drive the heating pipe 57 to slide on the outer surface of the guiding pipe 28, the heating pipe 57 will not only heat the culture fluid, but also prevent the guiding pipe 28 from bending, the heating pipe 57 can slide back and forth through the circulation of the pushing rod 54, the culture fluid in the guiding pipe 28 will flow stably and quickly along with the heating, let in the smooth entering cell culture bag 6 of culture solution, be favorable to avoiding the direction to appear crooked great, improve the culture solution mobility, let the culture solution avoid solidifying and blocking phenomenon through the heating methods.
In this embodiment, the cell culture bag 6 is connected to the lower end surface of the guide tube 28, the support groove 14 is connected to the lower end surface of the support plate 1, the support groove 14 is formed in a V-shape, and the outer surface of the cell culture bag 6 abuts against the inner surface of the support groove 14. The cell culture bag 6 is placed in the support groove 14 in advance to prevent the cell culture bag 6 from falling.
In this embodiment, a plurality of pushing rods 54 are arranged in a low-to-high circumferential arrangement around the upper end surface of the pushing plate 55, and the upper end surface of the second fixing plate 59 is disposed opposite to the lower end surface of the pushing plate 55. The round balls 53 on the lower end surface of the rotating part 52 are abutted against the pushing rods 54, the pushing rods 54 are arranged in a circle from low to high, each time the round balls 53 contact one pushing rod 54, the second spring body 58 between the first fixing plate 56 and the second fixing plate 59 is pressed, the second spring body 58 stretches downwards, the pushing plate 55 on the lower end surface of the pushing rod 54 slides downwards together with the second spring body 58 while pressing the second spring body 58, and the pushing plate 55 drives the heating pipe 57 to slide on the outer surface of the guide pipe 28, so that the situation that the guide is bent greatly is avoided, and the fluidity of the culture solution is improved.
The invention has the beneficial effects that:
firstly, the sucking disc 11 on the supporting plate 1 is selected to be adsorbed on the wall body or hung on a hook by the first through hole 12, the two fixing modes can also be used simultaneously, after the sucking disc 11 and the first through hole 12 are fixed, the cell culture bag 6 is placed in the supporting groove 14 in advance to avoid the cell culture bag 6 from falling off, then, the culture solution bottle 39 is placed on the outer surface of the clamping block 38 on the clamping mechanism 3, the culture solution bottle 39 is vertically clamped by people, the outer surface of the culture solution bottle 39 slides on the outer surfaces of the clamping blocks 38, the clamping blocks 38 can slide in the sliding groove 35 by the sliding part 36 through the volume of the culture solution bottle 39 and the clamping force given by people, the second clamping plates 37 can slide in the sliding groove 35 by the elasticity of the elastic band 381, the volume of the culture solution bottle 39 can be outwards expanded and wrapped, and the culture solution bottle 39 continues to vertically downwards, the culture solution bottle can be abutted to the outer surfaces of the first clamping plates 33, the first spring bodies 32 on the first clamping plates 33 stretch to clamp the outer surface of the culture solution bottle 39, the culture solution bottle 39 is connected to the connecting port 22, the culture solution bottle 39 is safely fixed, the working process of placing the culture solution bottle 39 is utilized, the culture solution bottles with different models can be installed and replenished with the culture solution, the applicability of an injection body is improved, and the working efficiency is improved.
After the culture solution bottle 39 is placed, the culture solution flows out of the bottle mouth into the inlet 22, flows into the inlet 23 through the guide of the inlet 22, and then flows into the inlet 23, and then flows through the inlet 23, the culture solution is filtered by the filter screen 25 and flows into the second housing 24, and the culture solution is opposite to the tip 43 on the contact rotation mechanism 4, the tip 43 allows the culture solution to flow vertically downward along the flow rod 41, at this time, the motor 412 starts to drive the rotation shaft 42 to rotate the flow rod 41, the first rotation plate 431 is rotated in the first rotation groove 241, and the culture solution under the rotation of the flow rod 41 flows rapidly and contacts the outer surfaces of the first rotation plate 431 and the second through hole 432 to stir and circulate, so as to prevent the culture solution from solidifying, and after the culture solution is stirred and circulated, the culture solution flows to the outer surface of the second rotation plate 44 along with the rotation of the flow rod 41, the culture solution is stirred and circulated again by the rotation of the second rotating plate 44 and the third through hole 441, the second rotating plate 44 drives the protection plate 45 to abut against the inner surface of the second accommodating shell 24, the culture solution is prevented from flowing out of the second rotating groove 242, the culture solution can be continuously kept in a liquid state by the stirring and circulation of the second rotating plate 44 and the third through hole 441, after passing through the third through hole 441 on the second rotating plate 44, the culture solution can flow downwards along with the outer surface of the bottom shell 411 until flowing into the inlet 27, the inlet 27 is guided into the guide pipe 28, the culture solution in the injection body is prevented from being solidified due to time change, the phenomenon that the culture solution channel is blocked or flows slowly is avoided, the fluidity of the culture solution is improved, and the culture solution is prevented from being solidified.
When the rotating mechanism 4 is started, the pushing mechanism 5 rotates together, the protective plate 45 drives the transmission circular plate 46 to rotate, the transmission circular plate 46 rotates in the second rotating groove 242, the transmission circular plate 46 drives the rotating disc 47, the rotating disc 47 rotates in the third rotating groove 211, the lower end of the outer side of the rotating disc 47 is transmitted to the transmission rod 51 on the pushing mechanism 5, the transmission rod 51 is driven by the rotation of the rotating disc 47, the round ball 53 on the lower end surface of the rotating part 52 abuts against the pushing rod 54, the pushing rods 54 are arranged in a circle from low to high, and each time the round ball 53 contacts one pushing rod 54, the second spring body 58 between the first fixing plate 56 and the second fixing plate 59 is pressed, the second spring body 58 stretches downwards, and when the second spring body 58 is pressed, the pushing plate 55 on the lower end surface of the pushing rod 54 slides downwards together with the second spring body 58, and the pushing plate 55 drives the heating pipe 57 to slide on the outer surface of the guide pipe 28 again, this heating pipe 57 not only can heat the culture solution, but also prevents the guide pipe 28 from bending, the circulation of the heating pipe 57 accessible pushing ram 54 slides back and forth, the culture solution in this guide pipe 28 is along with the stable and quick flow of heating, let the smooth entering cell culture bag 6 of culture solution in, it is great to be favorable to avoiding the direction to appear crooked, improve the culture solution mobility, let the culture solution avoid solidifying and blocking phenomenon through the heating mode.
The working principle is as follows:
firstly, the sucking disc 11 on the supporting plate 1 is selected to be adsorbed on the wall body or hung on the hook by the first through hole 12, or the two fixing modes can be simultaneously used, after the sucking disc 11 and the first through hole 12 are fixed, the cell culture bag 6 is placed in the supporting groove 14 in advance to avoid the cell culture bag 6 from falling off, then, the culture solution bottle 39 is placed on the outer surface of the clamping block 38 on the clamping mechanism 3, the culture solution bottle 39 is vertically clamped by a person downwards, the outer surface of the culture solution bottle 39 slides on the outer surfaces of the clamping blocks 38, the clamping blocks 38 can slide in the slide channel 35 by the volume of the culture solution bottle 39 and the clamping force of the person, then, the elastic band 381 can lead the second clamping plates 37 to slide in the slide channel 35 by the sliding part 36, the volume of the culture solution bottle 39 can be outwards expanded and clamped, and the culture solution bottle 39 continues to vertically downwards, the culture solution bottle can be abutted to the outer surfaces of the first clamping plates 33, the first spring bodies 32 on the first clamping plates 33 stretch to clamp the outer surface of the culture solution bottle 39, the culture solution bottle 39 is connected to the connecting port 22, the culture solution bottle 39 is safely fixed, the working process of placing the culture solution bottle 39 is utilized, the culture solution bottles with different models can be installed and replenished with the culture solution, the applicability of an injection body is improved, and the working efficiency is improved.
After the culture solution bottle 39 is placed, the culture solution flows out of the bottle mouth into the inlet 22, flows into the inlet 23 through the guide of the inlet 22, and then flows into the inlet 23, and then flows through the inlet 23, the culture solution is filtered by the filter screen 25 and flows into the second housing 24, and the culture solution is opposite to the tip 43 on the contact rotation mechanism 4, the tip 43 allows the culture solution to flow vertically downward along the flow rod 41, at this time, the motor 412 starts to drive the rotation shaft 42 to rotate the flow rod 41, the first rotation plate 431 is rotated in the first rotation groove 241, and the culture solution under the rotation of the flow rod 41 flows rapidly and contacts the outer surfaces of the first rotation plate 431 and the second through hole 432 to stir and circulate, so as to prevent the culture solution from solidifying, and after the culture solution is stirred and circulated, the culture solution flows to the outer surface of the second rotation plate 44 along with the rotation of the flow rod 41, the culture solution is stirred and circulated again by the rotation of the second rotating plate 44 and the third through hole 441, the second rotating plate 44 drives the protection plate 45 to abut against the inner surface of the second accommodating shell 24, the culture solution is prevented from flowing out of the second rotating groove 242, the culture solution can be continuously kept in a liquid state by the stirring and circulation of the second rotating plate 44 and the third through hole 441, after passing through the third through hole 441 on the second rotating plate 44, the culture solution can flow downwards along with the outer surface of the bottom shell 411 until flowing into the inlet 27, the inlet 27 is guided into the guide pipe 28, the culture solution in the injection body is prevented from being solidified due to time change, the phenomenon that the culture solution channel is blocked or flows slowly is avoided, the fluidity of the culture solution is improved, and the culture solution is prevented from being solidified.
When the rotating mechanism 4 is started, the pushing mechanism 5 rotates together, the protective plate 45 drives the transmission circular plate 46 to rotate, the transmission circular plate 46 rotates in the second rotating groove 242, the transmission circular plate 46 drives the rotating disc 47, the rotating disc 47 rotates in the third rotating groove 211, the lower end of the outer side of the rotating disc 47 is transmitted to the transmission rod 51 on the pushing mechanism 5, the transmission rod 51 is driven by the rotation of the rotating disc 47, the round ball 53 on the lower end surface of the rotating part 52 abuts against the pushing rod 54, the pushing rods 54 are arranged in a circle from low to high, and each time the round ball 53 contacts one pushing rod 54, the second spring body 58 between the first fixing plate 56 and the second fixing plate 59 is pressed, the second spring body 58 stretches downwards, and when the second spring body 58 is pressed, the pushing plate 55 on the lower end surface of the pushing rod 54 slides downwards together with the second spring body 58, and the catch bar 55 drives the heating pipe 57 again and slides on the surface of stand pipe 28, this heating pipe 57 not only can give the culture solution heating, still prevent that stand pipe 28 is crooked, the circulation of heating pipe 57 accessible catch bar 54 makes a round trip to slide, the culture solution in this stand pipe 28 is along with the stable and quick flow of heating, let in the smooth entering cell culture bag 6 of culture solution, it is great to be favorable to avoiding the direction bending to appear, improve the culture solution mobility, let the culture solution avoid solidifying and blocking phenomenon through the heating mode.
The embodiments of the present invention are disclosed as the preferred embodiments, but not limited thereto, and those skilled in the art can easily understand the spirit of the present invention and make various extensions and changes without departing from the spirit of the present invention.
Claims (7)
1. A cell culture bag for constant-current fluid infusion comprises a supporting plate (1) and is characterized in that one side of the supporting plate (1) is connected with a sucker (11), a first through hole (12) is formed in the upper portion of the supporting plate (1), one side of the supporting plate (1) is connected with a supporting rod (13), one end of the supporting rod (13) is connected with an injection body (2), the upper end of the injection body (2) is connected with a clamping mechanism (3), a rotating mechanism (4) is fixedly connected inside the injection body (2), and the lower end face of the rotating mechanism (4) is connected with a pushing mechanism (5); the lower end surface of the injection body (2) is connected with a cell culture bag (6).
2. A cell culture bag for constant flow apheresis as in claim 1, wherein: the injection body (2) comprises a first accommodating shell (21), one side of the outer surface of the first accommodating shell (21) is connected to one end of the supporting rod (13), the upper end face of the accommodating shell (21) is connected with an access port (22), the lower end face of the access port (22) is connected with a receiving port (23), the lower end face of the receiving port (23) is connected with a second accommodating shell (24), a filter screen (25) is arranged at the joint of the receiving port (23) and the second accommodating shell (24), the middle part of the lower end face of the second accommodating shell (24) is connected with a connecting plate (26), the lower end of the outer surface of the second accommodating shell (24) is connected to the inner surface of the lower end of the first accommodating shell (21), two sides of the connecting plate (26) are connected to two sides of the inner surface of the second accommodating shell (24), the lower end face of the second accommodating shell (24) is connected with a discharge port (27), and the discharge port (27) is opposite to the lower end port of the second accommodating shell (24), the discharge inlet (27) is opposite to the lower end surface of the connecting plate (26), and the lower end surface of the discharge inlet (27) is connected with a guide pipe (28).
3. A cell culture bag for constant flow apheresis as in claim 2, wherein: the clamping mechanism (3) comprises a supporting ring plate (31), the lower end surface of the supporting ring plate (31) is connected with the upper end of the outer surface of the first containing shell (21), first spring bodies (32) are uniformly distributed and surrounded on the inner surface of the supporting ring plate (31), one end of the first spring body (32) is connected with a first clamping plate (33), the upper end surface of the first clamping plate (33) is connected with a fixed plate (34), a slide way groove (35) is arranged in the middle of the upper end surface of the fixing plate (34), a sliding part (36) is connected in the slide way groove (35) in a sliding way, the upper end surface of the sliding part (36) is connected with a second clamping plate (37), the outer surface of one side of the second clamping plate (37) is connected with a clamping block (38), the second clamping plates (37) are connected with the plurality of second clamping plates (37) through elastic bands (381), and the outer surface of one side of the clamping block (38) is abutted with a culture solution bottle (39).
4. A cell culture bag for constant flow rehydration according to claim 3, wherein: the rotating mechanism (4) comprises a moving rod (41), the upper end of the moving rod (41) is connected to the inner upper portion of the second accommodating shell (24), the lower portion of the moving rod (41) is connected with a bottom shell (411), a motor (412) is connected to the bottom shell (411), the output end of the motor (412) is connected with a rotating shaft (42), one end of the rotating shaft (42) is connected to the lower end face of the moving rod (41), the moving rod (41) is in rotating connection and matching with the bottom shell (411), a tip (43) is arranged on the upper end face of the moving rod (41), a first rotating plate (431) is connected to the outer surface of the upper end of the moving rod (41), second through holes (432) are uniformly distributed in the first rotating plate (431), a first rotating groove (241) is rotatably connected to the first rotating plate (431), the inner surface of the upper end of the second accommodating shell (24) is opened in the first rotating groove (241), a second rotating plate (44) is connected to the outer surface of the lower end of the moving rod (41), third through holes (441) are formed in the second rotating plate (44), the outer surface of the second rotating plate (44) is connected with a protection plate (45), the outer surface of one side of the protection plate (45) abuts against the inner surface of the second accommodating shell (24), a transmission circular plate (46) is sleeved on the outer surface of the protection plate (45), the transmission circular plate (46) is rotatably connected with a second rotating groove (242), the second rotating groove (242) is formed in the middle of the second accommodating shell (24), the outer surface of the transmission circular plate (46) is connected with a rotating disc (47), the rotating disc (47) is rotatably connected with a third rotating groove (211), and the third rotating groove (211) is formed in the lower end of the first accommodating shell (21).
5. A cell culture bag for constant flow rehydration according to claim 4, wherein: the pushing mechanism (5) comprises a transmission rod (51), one end of the transmission rod (51) is connected to the lower end face of the rotating disc (47), the lower end face of the transmission rod (51) is connected with a rotating part (52), the lower end surface of the rotating part (52) is connected with a round ball (53), the outer surface of the round ball (53) is abutted with a push rod (54), the lower end surface of the push rod (54) is connected with a push plate (55), the lower end surface of the push plate (55) is connected with a first fixing plate (56), the middle part of the lower end surface of the pushing plate (55) is connected with a heating pipe (57), the pushing plate (55) and the heating pipe (57) penetrate through the outer surface of the guide pipe (28), the lower end surface of the first fixing plate (56) is connected with a second spring body (58), the lower end of the second spring body (58) is connected with a second fixing plate (59), and the second fixing plate (59) is fixed on the outer surface of the lower end of the guide pipe (28).
6. A cell culture bag for constant flow rehydration according to claim 5, wherein: the cell culture bag (6) is connected to the lower end face of the guide tube (28), the lower end face of the support plate (1) is connected with a support groove (14), the support groove (14) is V-shaped, and the outer surface of the cell culture bag (6) is abutted to the inner surface of the support groove (14).
7. A cell culture bag for constant flow rehydration according to claim 6, wherein: a plurality of pushing rods (54) are arranged in a low-to-high circumferential manner around the upper end surface of the pushing plate (55), and the upper end surface of the second fixing plate (59) is arranged opposite to the lower end surface of the pushing plate (55).
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| CN202210375342.6A CN114717113B (en) | 2022-04-11 | 2022-04-11 | Cell culture bag for constant-current fluid infusion |
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| CN202210375342.6A CN114717113B (en) | 2022-04-11 | 2022-04-11 | Cell culture bag for constant-current fluid infusion |
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| CN114717113B CN114717113B (en) | 2023-03-24 |
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| CN114958602A (en) * | 2022-04-21 | 2022-08-30 | 上海乐纯生物技术有限公司 | Cell therapy culture bag with filtering function |
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| US20190017009A1 (en) * | 2015-12-23 | 2019-01-17 | Shanghai GenBase Biotechnology Co., Ltd. | Fully automated continuous cell culture system |
| CN107586717A (en) * | 2017-10-31 | 2018-01-16 | 河南省肿瘤医院 | A kind of attachment means of cell culture bags and culture jar |
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| CN114717113B (en) | 2023-03-24 |
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