CN114717113B - Cell culture bag for constant-current fluid infusion - Google Patents

Cell culture bag for constant-current fluid infusion Download PDF

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Publication number
CN114717113B
CN114717113B CN202210375342.6A CN202210375342A CN114717113B CN 114717113 B CN114717113 B CN 114717113B CN 202210375342 A CN202210375342 A CN 202210375342A CN 114717113 B CN114717113 B CN 114717113B
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plate
rotating
face
rod
culture solution
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CN114717113A (en
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秦孙星
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Shanghai Lechunle New Material Co ltd
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Shanghai Lechunle New Material Co ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/14—Bags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/08—Flask, bottle or test tube
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/48—Holding appliances; Racks; Supports
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes

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  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
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  • Life Sciences & Earth Sciences (AREA)
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Abstract

A cell culture bag for constant-current fluid infusion comprises a supporting plate, wherein one side of the supporting plate is connected with a sucker, the upper part of the supporting plate is provided with a first through hole, one side of the supporting plate is connected with a supporting rod, one end of the supporting rod is connected with an injection body, the upper end of the injection body is connected with a clamping mechanism, the interior of the injection body is fixedly connected with a rotating mechanism, and the lower end face of the rotating mechanism is connected with a pushing mechanism; the lower end face of the injection body is connected with a cell culture bag, a sucker on the supporting plate is firstly selected to be adsorbed on a wall body or hung on a hook through a first through hole, the two fixing modes can be used simultaneously, and after the sucker and the first through hole are fixed, the cell culture bag is placed in a supporting groove in advance to avoid dropping of the cell culture bag.

Description

Cell culture bag for constant-current fluid infusion
Technical Field
The invention relates to the technical field of biological cell culture, in particular to a cell culture bag for constant-current fluid infusion.
Background
In the fields of immune cell therapy of tumors and scientific researches of biology, immunity, medicine and the like, a cell culture technology is often used and is also the basis of almost all cell biology experiments. During the cell culture period, the cells can be continuously grown and reach a certain number by passage and fluid replacement. To achieve higher numbers of cells, larger cell culture bags are often used. When a cell culture bag is used for culturing cells for passage or fluid replacement, a syringe of a 50ml syringe is generally used as a funnel and connected with the cell culture bag, and then culture fluid is poured into the syringe, and the culture fluid flows into the cell culture bag through the syringe to complete the passage or fluid replacement process.
Because current injection body only is fit for the culture solution bottle and installs, but the culture solution bottle of installation can only be with suitable model, too in singleness, can not install the fluid infusion by a plurality of different models, and also can not install bagged culture solution, cause and reduce the suitability of injection body, reduce work efficiency, culture solution in the injection body can solidify along with the change of time, cause the obstructed phenomenon of culture solution runner or flow slowly, and culture solution is in the pipe guide flow, the crooked great sometimes can appear in the pipe guide, cause the culture solution to flow slowly or blocking phenomenon.
In order to solve the problems, the invention provides a cell culture bag for constant-current fluid infusion.
Disclosure of Invention
(1) Technical problem to be solved
The invention aims to overcome the problems in the prior art, adapt to the practical needs, and provide a cell culture bag for constant-current fluid infusion so as to solve the technical problems.
(2) Technical scheme
In order to realize the purpose of the invention, the technical scheme adopted by the invention is as follows: a cell culture bag for constant-current fluid infusion comprises a supporting plate, wherein one side of the supporting plate is connected with a sucker, the upper part of the supporting plate is provided with a first through hole, one side of the supporting plate is connected with a supporting rod, one end of the supporting rod is connected with an injection body, the upper end of the injection body is connected with a clamping mechanism, the interior of the injection body is fixedly connected with a rotating mechanism, and the lower end face of the rotating mechanism is connected with a pushing mechanism; the lower end face of the injection body is connected with a cell culture bag.
Preferentially, the injection body comprises a first accommodating shell, one side of the outer surface of the first accommodating shell is connected to one end of the supporting rod, the upper end face of the accommodating shell is connected with an access port, the lower end face of the access port is connected with a second accommodating shell, a filter screen is installed at the joint of the access port and the second accommodating shell, the middle of the lower end face of the second accommodating shell is connected with a connecting plate, the lower end face of the outer surface of the second accommodating shell is connected to the inner surface of the lower end of the first accommodating shell, the two sides of the connecting plate are connected to the two sides of the inner surface of the second accommodating shell, the lower end face of the second accommodating shell is connected with a discharge port, the discharge port is opposite to the lower end face of the second accommodating shell, the discharge port is opposite to the lower end face of the connecting plate, and the lower end face of the discharge port is connected with a guide pipe.
Preferentially, fixture includes the supporting ring board, end connection is in the first surface upper end that holds the shell under this supporting ring board, and supporting ring inboard surface equipartition has first spring body around encircling, this first spring one end is connected with first grip block, this first grip block up end is connected with the fixed plate, the slide groove has been seted up at this fixed plate up end middle part, this slide inslot portion sliding connection has the sliding part, this sliding part up end is connected with the second grip block, this second grip block one side surface connection has the grip block, and the second grip block passes through the elastic cord and connects a plurality of second grip blocks, and grip block one side surface butt has the culture solution bottle.
Preferentially, the rotating mechanism comprises a flowing rod, the upper end of the flowing rod is connected to the upper inner portion of the second containing shell, the lower portion of the flowing rod is connected with a bottom shell, a motor is connected to the bottom shell, the output end of the motor is connected with a rotating shaft, one end of the rotating shaft is connected to the lower end face of the flowing rod, the flowing rod is in rotating connection and matching with the bottom shell, the upper end face of the flowing rod is provided with a tip, the outer surface of the upper end of the flowing rod is connected with a first rotating plate, second through holes are evenly distributed in the first rotating plate, a first rotating groove is connected to the outer surface of the first rotating plate in a rotating mode, the inner surface of the upper end of the second containing shell is formed in the first rotating groove, the outer surface of the lower end of the flowing rod is connected with a second rotating plate, a third through hole is formed in the second rotating plate, the outer surface of the second rotating plate is connected with a protection plate, the outer surface of one side of the protection plate abuts against the inner surface of the second containing shell, a transmission circular plate is sleeved with a transmission circular plate, the second rotation groove is formed in the middle of the second containing shell, and the second rotation groove is formed in the middle of the second containing shell.
Preferentially, pushing mechanism includes the transfer line, and this transfer line one end is connected at the lower terminal surface of rolling disc, and the transfer line lower terminal surface is connected with the rotation portion, and the terminal surface is connected with the ball under this rotation portion, and this ball surface butt has the catch bar, and the terminal surface is connected with the slurcam under this catch bar, and the terminal surface is connected with first fixed plate under this slurcam, and is connected with the heating pipe under the slurcam in the middle part of the terminal surface, and the slurcam runs through on the surface of stand pipe with the heating pipe, the terminal surface is connected with the second spring body under the first fixed plate, and this second spring body lower extreme is connected with the second fixed plate, and the lower extreme surface at the stand pipe is fixed to this second fixed plate.
Preferably, the cell culture bag is connected to a lower end surface of the guide tube, the lower end surface of the support plate is connected to a support groove having a V-shape, and an outer surface of the cell culture bag abuts against an inner surface of the support groove.
Preferably, the plurality of push rods are arranged in a low-to-high circumference around the upper end surface of the push plate, and the upper end surface of the second fixing plate is disposed opposite to the lower end surface of the push plate.
(3) Has the advantages that:
A. firstly, selecting a sucker on a supporting plate to be adsorbed on a wall body or hanging the sucker on a hook by using a first through hole, and simultaneously using the two fixing modes, wherein after the sucker and the first through hole are fixed, the cell culture bag is placed in a supporting groove in advance to prevent the cell culture bag from falling;
B. then, a culture solution bottle is placed on the outer surface of a clamping block on a clamping mechanism, a person downwards gives a vertical clamping force to the culture solution bottle, the outer surface of the culture solution bottle slides on the outer surfaces of a plurality of clamping blocks, the plurality of clamping blocks can pass through the volume of the culture solution bottle and the clamping force given by the person, and then the plurality of second clamping plates slide in a slide channel groove by using a sliding part through the elasticity of an elastic cord, so that the second clamping plates can outwards expand and wrap the culture solution bottle and clamp the culture solution bottle, the culture solution bottle continues to vertically downwards and can abut against the outer surfaces of the plurality of first clamping plates, a plurality of first spring bodies on the plurality of first clamping plates stretch and retract to clamp the outer surface of the culture solution bottle until the culture solution bottle abuts against an access port, and further the safe fixation of the culture solution bottle is completed;
C. after the culture solution bottle is placed, the culture solution flows into the access port from the bottle mouth, flows into the access port through the guide of the access port, and then flows into the access port, the culture solution can be filtered through the filter screen and flows into the second containing shell, the culture solution is just opposite to the tip on the contact rotating mechanism, the tip can enable the culture solution to vertically flow downwards along the flowing rod, the motor starts to start and drives the rotating shaft to enable the flowing rod to rotate, the first rotating plate can rotate in the first rotating groove, the culture solution can rapidly flow under the rotation of the flowing rod and can be in contact with the outer surfaces of the first rotating plate and the second through hole to be stirred and circulated, the solidification of the culture solution is prevented, and the culture solution is stirred and circulated again, the culture solution can flow to the outer surface of the second rotating plate along with the rotation of the flow rod, the culture solution is stirred and circulated again by utilizing the rotation of the second rotating plate and the third through hole, the second rotating plate can drive the protection plate to abut against the inner surface of the second containing shell, the culture solution is prevented from flowing out of the second rotating groove, the culture solution can be continuously kept in a liquid state by virtue of the stirring and the circulation of the second rotating plate and the third through hole, after passing through the third through hole in the second rotating plate, the culture solution can flow downwards along with the outer surface of the bottom shell until flowing into the inlet, and the inlet is guided into the guide pipe, so that the culture solution in the injection body can be solidified by avoiding the change of time, the phenomenon that a culture solution flow channel is blocked or slow to flow is avoided, the flowability of the culture solution is improved, and the solidification of the culture solution is prevented;
D. when the rotating mechanism is started, the pushing mechanism can rotate along with the heating of the culture medium, the transmission circular plate is driven to rotate through the protection plate, the transmission circular plate is driven to rotate in the second rotating groove, the transmission circular plate drives the rotating disc, the rotating disc rotates in the third rotating groove, the lower end of the outer side of the circle of the rotating disc can drive the transmission rod on the pushing mechanism, the transmission rod is driven by the rotating disc to rotate, the round balls on the lower end face of the rotating part abut against the push rod, the push rod is arranged in a low-to-high circumference, each round ball contacts one push rod, the second spring between the first fixing plate and the second fixing plate can be pressed, the second spring can stretch downwards, the push plate on the lower end face of the push rod can slide downwards along with the second spring at the same time of pressing of the second spring, the heating pipe can drive the heating pipe to slide on the outer surface of the guide pipe, the heating pipe can not only heat the culture medium, but also prevent the guide pipe from bending, the culture medium can slide downwards along with the smooth and flow of the heating pipe, the culture medium can be prevented from being heated and the culture medium can enter into the guide pipe to be heated, and the culture medium can be prevented from being rapidly and being blocked by the culture medium flowing back and forth, and the culture medium flowing, and the culture medium can be prevented from being heated.
Drawings
FIG. 1 is a schematic perspective view of a cell culture bag for constant flow fluid infusion according to the present invention;
FIG. 2 is a partially enlarged view of an injection body of a cell culture bag for constant flow fluid infusion according to the present invention;
FIG. 3 is a partially enlarged view of a clamping mechanism of a cell culture bag for constant flow fluid infusion according to the present invention;
FIG. 4 is a partially enlarged view of a rotating mechanism of a cell culture bag for constant flow fluid infusion according to the present invention;
FIG. 5 is a schematic diagram of the pushing mechanism of the cell culture bag for constant flow fluid infusion according to the present invention;
FIG. 6 is a schematic diagram of a cell culture bag for a constant flow fluid infusion cell culture bag according to the present invention.
The reference numbers are as follows:
1-support plate, 11-suction cup, 12-first through hole, 13-support rod, 14-support groove, 2-injection body, 21-first containing shell, 221-third rotating groove, 22-inlet, 23-inlet, 24-second containing shell, 241-first rotating groove, 242-second rotating groove, 25-filter screen, 26-connecting plate, 27-outlet, 28-guide tube, 3-clamping mechanism, 31-support ring plate, 32-first spring body, 33-first clamping plate, 34-fixed plate, 35-slide groove, 36-sliding part, 37-second clamping plate, 38-clamping block, 381-elastic band, 39-culture solution bottle, 4-rotating mechanism, 41-flow rod, 411-bottom shell, 412-motor, 42-rotating shaft, 43-tip, 431-first rotating plate, 432-second through hole, 44-second rotating plate, 441-third through hole, 45-protective plate, 46-transmission disk, 5-rotating disk, 5-pushing rotating rod, 53-pushing rod, 58-pushing rod, 55-ball, 53-pushing rod, 58-pushing rod, and 5-pushing rod.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
The invention will be further illustrated with reference to the following figures 1-6 and examples:
in this embodiment, as shown in fig. 1-6, a cell culture bag for constant-current fluid infusion includes a support plate 1, a suction cup 11 is connected to one side of the support plate 1, a first through hole 12 is formed in an upper portion of the support plate 1, a support rod 13 is connected to one side of the support plate 1, an injection body 2 is connected to one end of the support rod 13, a clamping mechanism 3 is connected to an upper end of the injection body 2, a rotating mechanism 4 is fixedly connected to an inside of the injection body 2, and a pushing mechanism 5 is connected to a lower end surface of the rotating mechanism 4; the lower end surface of the injection body 2 is connected with a cell culture bag 6. Firstly, the suction cup 11 on the supporting plate 1 is selected to be adsorbed on the wall body or hung on the hook by the first through hole 12, and the two fixing modes can also be used simultaneously.
In this embodiment, the injection body 2 includes a first accommodating case 21, one side of the outer surface of the first accommodating case 21 is connected to one end of the support rod 13, an inlet 22 is connected to the upper end surface of the accommodating case 21, an inlet 23 is connected to the lower end surface of the inlet 22, a second accommodating case 24 is connected to the lower end surface of the inlet 23, a filter screen 25 is installed at the connection position of the inlet 23 and the second accommodating case 24, a connecting plate 26 is connected to the middle of the lower end surface of the second accommodating case 24, the lower end surface of the outer surface of the second accommodating case 24 is connected to the inner surface of the lower end of the first accommodating case 21, two sides of the connecting plate 26 are connected to two sides of the inner surface of the second accommodating case 24, an outlet 27 is connected to the lower end surface of the second accommodating case 24, the outlet 27 is opposite to the lower end surface of the connecting plate 26, and a guide pipe 28 is connected to the lower end surface of the outlet 27. After placing culture solution bottle 39, the culture solution flows out of the bottle mouth to access port 22, flows into receiving port 23 through the guide flow of access port 22, and then flows into receiving port 23, and the culture solution can be filtered through filter screen 25 and flows into second containing shell 24, and the culture solution can flow downwards along with flow rod 41 and the outer surface of bottom shell 411 until flowing into discharging port 27, and the discharging port 27 is guided into guide pipe 28, so that impurities can be filtered, and the flow rate of the culture solution can be improved.
In this embodiment, the clamping mechanism 3 includes a supporting ring plate 31, the lower end surface of the supporting ring plate 31 is connected to the upper end of the outer surface of the first accommodating case 21, the inner surface of the supporting ring plate 31 is uniformly surrounded by a first spring body 32, one end of the first spring body 32 is connected to a first clamping plate 33, the upper end surface of the first clamping plate 33 is connected to a fixing plate 34, a chute 35 is formed in the middle of the upper end surface of the fixing plate 34, a sliding portion 36 is slidably connected to the inside of the chute 35, the upper end surface of the sliding portion 36 is connected to a second clamping plate 37, a clamping block 38 is connected to the outer surface of one side of the second clamping plate 37, the second clamping plate 37 is connected to a plurality of second clamping plates 37 through an elastic band 381, and a culture solution bottle 39 is abutted to the outer surface of one side of the clamping block 38. Then, the culture solution bottle 39 is placed on the outer surface of the clamping block 38 on the clamping mechanism 3, the culture solution bottle 39 is vertically clamped by a person downwards, the outer surface of the culture solution bottle 39 slides on the outer surfaces of the clamping blocks 38, the clamping blocks 38 can pass through the volume of the culture solution bottle 39 and the clamping force given by the person, and the second clamping plates 37 slide inside the slide channel groove 35 by using the sliding part 36 through the elasticity of the elastic band 381, the volume of the culture solution bottle 39 can be outwards expanded and wrapped and clamped, the culture solution bottle 39 continues to vertically downwards and can be abutted against the outer surfaces of the first clamping plates 33, the first spring bodies 32 on the first clamping plates 33 stretch and retract to clamp the outer surface of the culture solution bottle 39 until the culture solution bottle 39 is abutted to the access port 22, so that the safe fixation of the culture solution bottle 39 is completed, and the working process of placing the culture solution bottle 39 is beneficial to installing and supplementing solutions of different types, the applicability of injection bodies is improved, and the working efficiency is improved.
In this embodiment, the rotating mechanism 4 includes a flow rod 41, an upper end of the flow rod 41 is connected to an upper inner portion of the second accommodating case 24, a bottom case 411 is connected to a lower portion of the flow rod 41, a motor 412 is connected to the bottom case 411, an output end of the motor 412 is connected to a rotating shaft 42, one end of the rotating shaft 42 is connected to a lower end surface of the flow rod 41, the flow rod 41 is rotatably connected to the bottom case 411, a tip 43 is disposed on an upper end surface of the flow rod 41, a first rotating plate 431 is connected to an outer surface of an upper end of the flow rod 41, second through holes 432 are uniformly disposed in the first rotating plate 431, the first rotating plate 431 is rotatably connected to a first rotating groove 241, the first rotating groove 241 is disposed on an inner surface of an upper end of the second accommodating case 24, a second rotating plate 44 is connected to an outer surface of a lower end of the flow rod 41, a third through hole 441 is disposed in the second rotating plate 44, a shielding plate 45 is connected to an outer surface of the second rotating plate 44, an outer surface of one side of the shielding plate 45 abuts against an inner surface of the second accommodating case 24, a transmission disk 46 is sleeved on the outer surface of the shielding plate 45, a transmission disk 47 is connected to a middle portion of the second accommodating case 211, and a middle of the second accommodating case 211 is connected to the transmission disk 211. The culture solution is filtered by the filter screen 25 and flows into the second containing shell 24, and the culture solution is opposite to the top 43 on the contact rotating mechanism 4, the top 43 allows the culture solution to flow vertically downwards along the flow rod 41, at this time, the motor 412 starts to be started, and drives the rotating shaft 42 to rotate the flow rod 41, the first rotating plate 431 is rotated in the first rotating groove 241, and the culture solution is rapidly flowed by the rotation of the flow rod 41, and is contacted with the outer surfaces of the first rotating plate 431 and the second through hole 432 for stirring and flowing, so as to prevent the culture solution from solidifying, and after the culture solution is further circulated by stirring, the culture solution flows to the outer surface of the second rotating plate 44 along with the rotation of the flow rod 41, the culture solution is further stirred and circulated by the rotation of the second rotating plate 44 and the third through hole 441, and the second rotating plate 44 drives the protection plate 45 to abut against the inner surface of the second containing shell 24, the culture solution is prevented from flowing out of the second rotating groove 242, and the culture solution can continuously keep a liquid state by the stirring and circulation of the second rotating plate 44 and the third through hole 441, after passing through the third through hole 441 on the second rotating plate 44, the culture solution can flow downward along with the outer surface of the bottom shell 411 until flowing into the discharging port 27, the discharging port 27 is guided into the guide pipe 28, the pushing mechanism 5 can rotate along with the rotating mechanism 4 when the rotating mechanism 4 is started, the transmission circular plate 46 is driven by the protection plate 45 to rotate, the transmission circular plate 46 rotates in the second rotating groove 242, and the transmission circular plate 46 drives the rotating disc 47 to rotate in the third rotating groove 211, thereby being beneficial to preventing the culture solution in the injection body from being solidified due to time change, avoiding obstruction of a culture solution flow channel or slow flowing, and improving the fluidity of the culture solution, prevent the culture solution from coagulating.
In this embodiment, the pushing mechanism 5 includes a transmission rod 51, one end of the transmission rod 51 is connected to the lower end surface of the rotating disc 47, the lower end surface of the transmission rod 51 is connected to a rotating portion 52, the lower end surface of the rotating portion 52 is connected to a round ball 53, the outer surface of the round ball 53 is abutted to a pushing rod 54, the lower end surface of the pushing rod 54 is connected to a pushing plate 55, the lower end surface of the pushing plate 55 is connected to a first fixing plate 56, the middle portion of the lower end surface of the pushing plate 55 is connected to a heating pipe 57, an electric heating wire is wound around the inside of the heating pipe 57, the pushing plate 55 and the heating pipe 57 penetrate through the outer surface of the guide tube 28, the lower end surface of the first fixing plate 56 is connected to a second spring body 58, the lower end of the second spring body 58 is connected to a second fixing plate 59, and the second fixing plate 59 is fixed to the outer surface of the lower end of the guide tube 28. The lower end of the outer side of the rotating disc 47 is transmitted to the transmission rod 51 on the pushing mechanism 5, the transmission rod 51 is driven by the rotation of the rotating disc 47, the round balls 53 on the lower end surface of the rotating part 52 are abutted to the push rods 54, the push rods 54 are arranged in a low-to-high circle, each time the round balls 53 contact one push rod 54, the second spring body 58 between the first fixing plate 56 and the second fixing plate 59 is pressed, the second spring body 58 stretches downwards, the push plate 55 on the lower end surface of the push rod 54 and the second spring body 58 slide downwards simultaneously while pressing the second spring body 58, the push plate 55 drives the heating pipe 57 to slide on the outer surface of the guide pipe 28, the heating pipe 57 not only heats the culture solution, but also prevents the guide pipe 28 from bending, the heating pipe 57 can slide back and forth through the circulation of the push rods 54, the culture solution in the guide pipe 28 flows stably and quickly along with the heating, so that the culture solution enters the cell culture bag 6 smoothly, the guide bending is prevented, the culture solution is prevented from being heated, and the culture solution is prevented from being solidified and blocked by a heating mode and a heating liquid flow property is prevented from being blocked.
In this embodiment, the cell culture bag 6 is connected to the lower end surface of the guide tube 28, the support groove 14 is connected to the lower end surface of the support plate 1, the support groove 14 is formed in a V-shape, and the outer surface of the cell culture bag 6 abuts against the inner surface of the support groove 14. The cell culture bag 6 is placed in the support tank 14 in advance to prevent the cell culture bag 6 from falling.
In this embodiment, a plurality of pushing rods 54 are arranged in a low-to-high circumferential arrangement around the upper end surface of the pushing plate 55, and the upper end surface of the second fixing plate 59 is disposed opposite to the lower end surface of the pushing plate 55. The round balls 53 on the lower end surface of the rotating part 52 are abutted against the pushing rods 54, the pushing rods 54 are arranged in a circle from low to high, each time the round balls 53 contact one pushing rod 54, the second spring body 58 between the first fixing plate 56 and the second fixing plate 59 is pressed, the second spring body 58 stretches downwards, the pushing plate 55 on the lower end surface of the pushing rod 54 slides downwards together with the second spring body 58 while pressing the second spring body 58, and the pushing plate 55 drives the heating pipe 57 to slide on the outer surface of the guide pipe 28, so that the situation that the guide is bent greatly is avoided, and the fluidity of the culture solution is improved.
The invention has the beneficial effects that:
firstly, the sucking disc 11 on the supporting plate 1 is selected to be adsorbed on a wall body or hung on a hook by using the first through hole 12, and the two fixing modes can be simultaneously used, after the sucking disc 11 and the first through hole 12 are fixed, the cell culture bag 6 is placed in the supporting groove 14 in advance to avoid the cell culture bag 6 from falling off, then, a culture solution bottle 39 is placed on the outer surface of a clamping block 38 on the clamping mechanism 3, the culture solution bottle 39 is vertically clamped by a person downwards, the outer surface of the culture solution bottle 39 slides on the outer surfaces of a plurality of clamping blocks 38, the plurality of clamping blocks 38 can abut against the outer surfaces of a plurality of first clamping plates 33 through the volume of the culture solution bottle 39 and the clamping force given by the person through the elasticity of an elastic band 381, a plurality of second clamping plates 37 slide in a sliding channel groove 35 by using a sliding part 36, the volume of the culture solution bottle 39 can be outwards expanded and wrapped and clamped, the culture solution bottle 39 continues to vertically downwards, the outer surfaces of a plurality of first spring bodies 32 on the plurality of first clamping plates 33 can be clamped until the outer surfaces of the culture solution bottle 39 is stretched, the culture solution bottle is placed on the outer surface, the culture solution bottle 39, the culture solution bottle is beneficial to improve the safety of the working solution bottle 39, and the working efficiency of the culture solution bottle 39 can be improved, and the culture solution bottle can be fixed by using the safety of the culture solution bottle 39.
After the culture solution bottle 39 is placed, the culture solution flows out of the bottle mouth into the inlet 22, flows into the inlet 23 through the guide of the inlet 22, and then flows into the inlet 23, the culture solution is filtered by the filter screen 25 and flows into the second housing 24, the culture solution is opposite to the tip 43 on the contact rotation mechanism 4, the tip 43 allows the culture solution to vertically flow downwards along the flow rod 41, at this time, the motor 412 starts to start, the rotation shaft 42 is driven to rotate the flow rod 41, the first rotation plate 431 is rotated in the first rotation groove 241, the culture solution is rapidly flowed by the rotation of the flow rod 41, and is contacted with the outer surfaces of the first rotation plate 431 and the second through hole 432 to be stirred and circulated, the culture solution is prevented from being solidified, and the culture solution is stirred and circulated, the culture solution flows to the outer surface of the second rotating plate 44 along with the rotation of the flow rod 41, the culture solution is stirred and circulated again by the rotation of the second rotating plate 44 and the third through hole 441, the second rotating plate 44 drives the protection plate 45 to abut against the inner surface of the second accommodating shell 24, the culture solution is prevented from flowing out of the second rotating groove 242, the culture solution can continuously keep a liquid state by the stirring and circulation of the second rotating plate 44 and the third through hole 441, the culture solution flows downwards along with the outer surface of the bottom shell 411 after passing through the third through hole 441 on the second rotating plate 44 until flowing into the discharge port 27, and the discharge port 27 is guided into the guide tube 28, so that the culture solution in the injection body is prevented from being solidified due to the change of time, the phenomenon that the culture solution does not flow in a flow channel or flows slowly, the fluidity of the culture solution is improved, and the solidification of the culture solution is prevented.
When the rotating mechanism 4 is started, the pushing mechanism 5 rotates along with the rotating mechanism, the protective plate 45 drives the transmission circular plate 46 to rotate, the transmission circular plate 46 rotates in the second rotating groove 242, the transmission circular plate 46 drives the rotating disc 47, the rotating disc 47 rotates in the third rotating groove 211, the lower end of the outer side of the rotating disc 47 is driven to transmit the transmission rod 51 on the pushing mechanism 5, the transmission rod 51 is driven by the rotation of the rotating disc 47, the round ball 53 on the lower end face of the rotating part 52 abuts against the pushing rod 54, the pushing rods 54 are arranged in a low-to-high circumference, and each time the round ball 53 contacts one pushing rod 54, the second spring 58 between the first fixing plate 56 and the second fixing plate 59 is pressed, the second spring 58 stretches downward, the pushing plate 55 simultaneously slides downward with the second spring 58 while pressing the second spring 58, the pushing plate 55 drives the heating pipe 57 to slide on the outer surface of the guiding pipe 28, the heating pipe 57 not only provides the heating liquid to the heating pipe 28, but also prevents the heating liquid from flowing back and forth, the heating liquid from flowing through the guiding pipe 28, and prevents the heating liquid from flowing and flowing back and forth, and flowing through the guiding pipe 6, and the culture medium from being blocked.
The working principle is as follows:
firstly, the sucking disc 11 on the supporting plate 1 is selected to be adsorbed on the wall body or hung on the hook by the first through hole 12, and the two fixing modes can also be used simultaneously, after the sucking disc 11 and the first through hole 12 are fixed, the cell culture bag 6 is placed in the supporting groove 14 in advance to avoid the cell culture bag 6 from falling off, then, the culture solution bottle 39 is placed on the outer surface of the clamping block 38 on the clamping mechanism 3, the culture solution bottle 39 is vertically clamped by a person downwards, the outer surface of the culture solution bottle 39 slides on the outer surfaces of the clamping blocks 38, the clamping blocks 38 pass through the volume of the culture solution bottle 39 and the clamping force given by the person, and then the tightness of the elastic band 381, let a plurality of second grip blocks 37 utilize the slider 36 to slide inside the chute 35, can outwards expand the volume and the centre gripping of parcel in the culture solution bottle 39, and the culture solution bottle 39 continues perpendicularly downwards, can butt in the surface of a plurality of first grip blocks 33, let a plurality of first spring bodies 32 on a plurality of first grip blocks 33 stretch out and draw back and carry out the surface of centre gripping culture solution bottle 39, until culture solution bottle 39 connects to on connecing the mouth 22, and then accomplish culture solution bottle 39 safety fastening, utilize the course of work of placing culture solution bottle 39, be favorable to letting the nutrient solution bottle of different models install the fluid infusion, and improve the suitability of injection body, improve work efficiency.
After the culture solution bottle 39 is placed, the culture solution flows out of the bottle mouth into the inlet 22, flows into the inlet 23 through the guide of the inlet 22, and then flows into the inlet 23, the culture solution is filtered by the filter screen 25 and flows into the second housing 24, the culture solution is opposite to the tip 43 on the contact rotation mechanism 4, the tip 43 allows the culture solution to vertically flow downwards along the flow rod 41, at this time, the motor 412 starts to start, the rotation shaft 42 is driven to rotate the flow rod 41, the first rotation plate 431 is rotated in the first rotation groove 241, the culture solution is rapidly flowed by the rotation of the flow rod 41, and is contacted with the outer surfaces of the first rotation plate 431 and the second through hole 432 to be stirred and circulated, the culture solution is prevented from being solidified, and the culture solution is stirred and circulated, the culture solution flows to the outer surface of the second rotating plate 44 along with the rotation of the flow rod 41, the culture solution is stirred and circulated again by the rotation of the second rotating plate 44 and the third through hole 441, the second rotating plate 44 drives the protection plate 45 to abut against the inner surface of the second accommodating shell 24, the culture solution is prevented from flowing out of the second rotating groove 242, the culture solution can continuously keep a liquid state by the stirring and circulation of the second rotating plate 44 and the third through hole 441, the culture solution flows downwards along with the outer surface of the bottom shell 411 after passing through the third through hole 441 on the second rotating plate 44 until flowing into the discharge port 27, and the discharge port 27 is guided into the guide tube 28, so that the culture solution in the injection body is prevented from being solidified due to the change of time, the phenomenon that the culture solution does not flow in a flow channel or flows slowly, the fluidity of the culture solution is improved, and the solidification of the culture solution is prevented.
When the rotating mechanism 4 is started, the pushing mechanism 5 rotates along with the rotating mechanism, the protective plate 45 drives the transmission circular plate 46 to rotate, the transmission circular plate 46 rotates in the second rotating groove 242, the transmission circular plate 46 drives the rotating disc 47, the rotating disc 47 rotates in the third rotating groove 211, the lower end of the outer side of the rotating disc 47 is driven to transmit the transmission rod 51 on the pushing mechanism 5, the transmission rod 51 is driven by the rotation of the rotating disc 47, the round ball 53 on the lower end face of the rotating part 52 abuts against the pushing rod 54, the pushing rods 54 are arranged in a low-to-high circumference, and each time the round ball 53 contacts one pushing rod 54, the second spring 58 between the first fixing plate 56 and the second fixing plate 59 is pressed, the second spring 58 stretches downward, the pushing plate 55 simultaneously slides downward with the second spring 58 while pressing the second spring 58, the pushing plate 55 drives the heating pipe 57 to slide on the outer surface of the guiding pipe 28, the heating pipe 57 not only provides the heating liquid to the heating pipe 28, but also prevents the heating liquid from flowing back and forth, the heating liquid from flowing through the guiding pipe 28, and prevents the heating liquid from flowing and flowing back and forth, and flowing through the guiding pipe 6, and the culture medium from being blocked.
The embodiments of the present invention are disclosed as the preferred embodiments, but not limited thereto, and those skilled in the art can easily understand the spirit of the present invention and make various extensions and changes without departing from the spirit of the present invention.

Claims (1)

1. A cell culture bag for constant-current fluid infusion comprises a supporting plate (1) and is characterized in that one side of the supporting plate (1) is connected with a sucker (11), a first through hole (12) is formed in the upper portion of the supporting plate (1), one side of the supporting plate (1) is connected with a supporting rod (13), one end of the supporting rod (13) is connected with an injection body (2), the upper end of the injection body (2) is connected with a clamping mechanism (3), a rotating mechanism (4) is fixedly connected inside the injection body (2), and the lower end face of the rotating mechanism (4) is connected with a pushing mechanism (5); the lower end surface of the injection body (2) is connected with a cell culture bag (6); the injection body (2) comprises a first accommodating shell (21), one side of the outer surface of the first accommodating shell (21) is connected to one end of the supporting rod (13), the upper end face of the accommodating shell (21) is connected with an access port (22), the lower end face of the access port (22) is connected with a receiving port (23), the lower end face of the receiving port (23) is connected with a second accommodating shell (24), a filter screen (25) is installed at the joint of the receiving port (23) and the second accommodating shell (24), the middle part of the lower end face of the second accommodating shell (24) is connected with a connecting plate (26), the lower end of the outer surface of the second accommodating shell (24) is connected to the inner surface of the lower end of the first accommodating shell (21), two sides of the connecting plate (26) are connected to two sides of the inner surface of the second accommodating shell (24), the lower end face of the second accommodating shell (24) is connected with a discharge port (27), the discharge port (27) is right opposite to the lower end face of the second accommodating shell (24), the discharge port (27) is right opposite to the lower end face of the connecting plate (26), and the discharge port (27) is connected with a guide pipe (28); the clamping mechanism (3) comprises a supporting ring plate (31), the lower end face of the supporting ring plate (31) is connected to the upper end of the outer surface of the first accommodating shell (21), first spring bodies (32) are uniformly distributed on the inner surface of the supporting ring plate (31) in a surrounding mode, one end of each first spring body (32) is connected with a first clamping plate (33), the upper end face of each first clamping plate (33) is connected with a fixing plate (34), the middle of the upper end face of each fixing plate (34) is provided with a chute groove (35), a sliding part (36) is slidably connected inside each chute groove (35), the upper end face of each sliding part (36) is connected with a second clamping plate (37), the outer surface of one side of each second clamping plate (37) is connected with a clamping block (38), each second clamping plate (37) is connected with a plurality of second clamping plates (37) through elastic bands (381), and the outer surface of one side of each clamping block (38) is abutted to a culture solution bottle (39); the rotating mechanism (4) comprises a flowing rod (41), the upper end of the flowing rod (41) is connected to the inner upper part of the second accommodating shell (24), the lower part of the flowing rod (41) is connected with a bottom shell (411), a motor (412) is connected to the bottom shell (411), the output end of the motor (412) is connected with a rotating shaft (42), one end of the rotating shaft (42) is connected to the lower end face of the flowing rod (41), the flowing rod (41) is in rotating connection and matching with the bottom shell (411), the upper end face of the flowing rod (41) is provided with a top (43), the outer surface of the upper end of the flowing rod (41) is connected with a first rotating plate (431), second through holes (432) are uniformly distributed in the first rotating plate (431), the first rotating plate (431) is rotatably connected with a first rotating groove (241), the first rotating groove (241) is arranged on the inner surface of the upper end of the second accommodating shell (24), the outer surface of the lower end of the flowing rod (41) is connected with a second rotating plate (44), a second rotating plate (44) is connected with a second rotating protection plate (45), and a transmission plate (46) is sleeved on one side of the outer surface of the second rotating plate (24), the second rotating groove (242) is arranged in the middle of the second accommodating shell (24), the outer surface of the transmission circular plate (46) is connected with a rotating disc (47), the rotating disc (47) is rotatably connected with a third rotating groove (211), and the third rotating groove (211) is arranged at the lower end of the first accommodating shell (21); the pushing mechanism (5) comprises a transmission rod (51), one end of the transmission rod (51) is connected to the lower end face of the rotating disc (47), the lower end face of the transmission rod (51) is connected with a rotating part (52), the lower end face of the rotating part (52) is connected with a round ball (53), the outer surface of the round ball (53) is abutted with a pushing rod (54), the lower end face of the pushing rod (54) is connected with a pushing plate (55), the lower end face of the pushing plate (55) is connected with a first fixing plate (56), the middle part of the lower end face of the pushing plate (55) is connected with a heating pipe (57), the pushing plate (55) and the heating pipe (57) penetrate through the outer surface of the guide pipe (28), the lower end face of the first fixing plate (56) is connected with a second spring body (58), the lower end of the second spring body (58) is connected with a second fixing plate (59), and the second fixing plate (59) is fixed on the outer surface of the lower end of the guide pipe (28);
the cell culture bag (6) is connected to the lower end face of the guide tube (28), the lower end face of the support plate (1) is connected with a support groove (14), the support groove (14) is V-shaped, and the outer surface of the cell culture bag (6) is abutted against the inner surface of the support groove (14); a plurality of pushing rods (54) are arranged in a low-to-high circumferential manner around the upper end surface of the pushing plate (55), and the upper end surface of the second fixing plate (59) is arranged opposite to the lower end surface of the pushing plate (55).
CN202210375342.6A 2022-04-11 2022-04-11 Cell culture bag for constant-current fluid infusion Active CN114717113B (en)

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WO2017107837A1 (en) * 2015-12-23 2017-06-29 上海吉凯基因科技有限公司 Fully automated continuous cell culture system
CN107586717B (en) * 2017-10-31 2024-03-26 河南省肿瘤医院 Connecting device for cell culture bag and culture solution bottle
CN210596094U (en) * 2019-07-09 2020-05-22 武汉汉密顿生物科技股份有限公司 Cell fluid infusion device
CN214457881U (en) * 2020-12-08 2021-10-22 厦门三一造血技术有限公司 Cell fluid infusion and fluid taking bracket
CN214422611U (en) * 2021-01-18 2021-10-19 山东省齐鲁干细胞工程有限公司 Quantitative liquid supplementing and taking device for cell culture bag

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