CN218755693U - Nucleic acid extraction instrument - Google Patents

Abstract

The utility model discloses a nucleic acid extraction instrument relates to nucleic acid extraction equipment technical field. The utility model discloses a base station, be provided with loading mechanism, application of sample mechanism, wiper mechanism and elution mechanism on the base station, rotate on the base station and be connected with the reaction dish, the standing groove has been seted up on the reaction dish. The utility model discloses a put into fixed position with the sample, carry out automatic application of sample by sample application of sample subassembly and reagent application of sample subassembly, save artifical link, reduce intensity of labour, just the utility model discloses utilize hopper, sprocket, load mutually supporting of passageway isotructure, can load reaction cup and automatic uninstallation abandonment reaction cup automatically, avoid the manual work to frequently get the kit and put, further realize automaticly, the utility model discloses can incessantly draw the sample in succession, can shut down at any time midway and add reagent and sample, be convenient for improve nucleic acid extraction efficiency, can define the quantity of test in a flexible way, accomplish the sample along with examining.

Description

Nucleic acid extraction instrument

Technical Field

The utility model relates to a nucleic acid extraction equipment technical field specifically is a nucleic acid extraction instrument

Background

Nucleic acid refers to a biological macromolecular compound formed by polymerizing a plurality of nucleotides, which is one of the most basic substances of life, the magnetic bead method is commonly adopted for extracting nucleic acid at present, the magnetic bead method is a novel nucleic acid extraction technology taking nano biological magnetic beads as carriers, nucleic acid molecules can be specifically identified and combined with silicon hydroxyl on the surfaces of the magnetic beads, and aggregation or dispersion is carried out under the action of an external magnetic field, so that the nucleic acid is separated and purified. At present, for the extraction of DNA, the magnetic bead method nucleic acid extraction generally comprises four main steps of cracking, combining, washing and eluting, each step can be realized by a plurality of different methods singly or jointly, and a nucleic acid extractor is an instrument for automatically finishing the work of extracting sample nucleic acid by using a matched nucleic acid extraction reagent, and is widely applied to various fields of disease control centers, clinical disease diagnosis, blood transfusion safety, forensic medicine identification, environmental microorganism detection, food safety detection, animal husbandry, molecular biology research and the like.

When nucleic acid is extracted by the existing nucleic acid extractor on the market, the nucleic acid can be extracted sequentially according to batches, 8/16/32/64/96 samples are extracted in each batch, and after extraction is finished, a kit, a magnetic rod sleeve and the like need to be replaced manually by stopping the machine, so that the extraction efficiency is low, and the automation degree is poor.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a nucleic acid extraction appearance for draw the sample automatically, avoid the manual work frequently to get the kit and put, further realize automaticly.

In order to achieve the above object, the utility model provides a following technical scheme: a nucleic acid extractor, comprising:

the device comprises a base station, a reaction disc and a positioning device, wherein the base station is rotatably connected with the reaction disc, and the reaction disc is provided with a placing groove;

the loading mechanism is arranged on the base station and is used for automatically loading the reaction cups into the placing grooves on the reaction disc;

a sample adding mechanism arranged on the base station and used for adding a sample solution and a reagent into a reaction cup loaded on a reaction disc;

the cleaning mechanism is arranged on the base station and used for cleaning impurities in the sample solution mixed with the reagent in the reaction cup;

and an elution mechanism provided on the base for separating the magnetic beads and the nucleic acids from each other in the washed sample solution.

Furthermore, a control assembly is arranged on the base station, and the control assembly is electrically connected with the loading mechanism, the sample adding mechanism, the cleaning mechanism and the eluting mechanism.

Furthermore, the loading mechanism comprises a hopper arranged on the base station, a support connected inside the hopper, a chain wheel rotatably connected on the support, a chain movably connected on the chain wheel, a baffle arranged on the chain, a loading channel arranged on the support, a full cup photoelectric switch arranged on one side of the upper end of the loading channel, a positive and negative cup photoelectric switch arranged on the other side of the loading channel close to the upper end position, wherein the full cup photoelectric switch is arranged above the positive and negative cup photoelectric switch, a positive and negative cup assembly arranged on the loading channel close to the middle position, a blocking electromagnet arranged on the side wall of the loading channel, and a releasing electromagnet arranged on the side wall of the loading channel.

Further, application of sample mechanism is including setting up frame on the base station sets up sample application of sample subassembly on the frame, setting are in reagent application of sample subassembly, setting on the base station are in box, setting are placed to sample on the base station and are in box, setting are placed to lysate on the base station and are in magnetic bead mixing subassembly on the base station.

Furthermore, the cleaning mechanism comprises a fixed seat fixedly connected to the base platform, a movable frame movably connected to the side wall of the fixed seat, a cleaning needle fixing plate arranged on the movable frame, a cleaning drawing needle arranged on the cleaning needle fixing plate, and a cleaning punching needle arranged on the base platform.

Further, the elution mechanism comprises a suction needle arranged on the cleaning needle fixing plate, an eluent injection needle supporting frame arranged on the base platform, an eluent injection needle arranged on the eluent injection needle supporting frame, a first vibration assembly arranged on the base platform and a purification recovery box arranged on the base platform.

Furthermore, the sample adding assembly comprises an X arm fixedly connected to the base, a Y arm movably connected to the X arm, a Z arm movably connected to the Y arm, a liquid transfer pump mounting rack movably connected to the Z arm, a liquid transfer pump arranged on the liquid transfer pump mounting rack, and a TIP head arranged on the liquid transfer pump.

Further, the sample loading assembly comprises an X arm fixedly connected to the control assembly, a Y arm movably connected to the X arm, a Z arm movably connected to the Y arm, and a sample needle arranged on the Z arm.

Furthermore, reagent application of sample subassembly includes fixed connection first base on the base station, rotate to connect in pivot on the first base, connect in epaxial reagent application of sample arm, setting are in reagent needle on the reagent application of sample arm.

Further, the magnetic bead mixing subassembly includes fixed connection and is in second base on the base station, setting are in seat, setting are placed to the magnetic bead bottle on the second base action wheel on the second base, setting are in the magnetic bead bottle on the seat is placed to the magnetic bead bottle.

The utility model discloses possess following beneficial effect at least:

1. common nucleic acid extraction appearance on the market needs to manually add the sample into the kit, then put the kit into the instrument for testing, and the utility model discloses a put the sample into fixed position, carry out automatic application of sample by sample application of sample subassembly and reagent application of sample subassembly, save artifical link, reduce intensity of labour;

2. a common nucleic acid extraction instrument on the market usually uses a 96-deep-hole plate or a single test strip, needs to be manually placed and needs to be manually taken out of a kit after the test is finished, and the utility model utilizes the structures such as a hopper, a chain wheel, a loading channel and the like to automatically load a reaction cup and automatically unload a waste reaction cup, thereby avoiding the frequent taking and placing of the kit by manual work and further realizing automation;

3. a common nucleic acid extraction instrument on the market usually adopts a 96-deep-hole plate or a single test strip and a matched magnetic rod sleeve, which all belong to disposable consumables, and the utility model adopts a single Kong Fanying cup, which has small volume and no other solid wastes, thereby greatly saving the consumable cost;

4. a96-deep-hole plate or a single test strip is usually adopted in a common nucleic acid extraction instrument in the market, the temperature control of each reaction hole position is difficult to realize, the instrument adopts an independent single reaction cup mode, each reaction cup moves in different areas in sequence, the temperature control of each area is convenient to realize by using an electric heater, and the nucleic acid purification rate is improved;

5. common nucleic acid extraction appearance on the market adopts 96 deep hole boards or single test strip, need directly encapsulate required reagent in corresponding test hole, and encapsulation equipment is complicated, and the requirement is higher, and the utility model discloses use bottled reagent, the encapsulation is simple.

6. The common nucleic acid extractor on the market can only extract 1-96 samples each time, the extraction time is 30-60min each time, and the manual replacement of the reagent kit, the magnetic rod sleeve and the like is needed after the extraction of all samples is finished, so that the utility model can continuously extract samples, and can be stopped to add reagents and samples in the midway at any time, thereby being convenient for improving the nucleic acid extraction efficiency;

7. the common nucleic acid extractor on the market extracts 8/16/32/64/96 samples in each batch, and the utility model discloses a single reaction cup mode can define the quantity of test in a flexible way, accomplishes the sample and follows along with examining.

Of course, it is not necessary for any particular product to achieve all of the above-described advantages at the same time.

Drawings

Fig. 1 is a first schematic top view of the overall structure of the present invention;

fig. 2 is a second schematic top view of the overall structure of the present invention;

FIG. 3 is a schematic sectional view of the reaction plate structure of the present invention;

fig. 4 is a first perspective view of the overall structure of the present invention;

FIG. 5 is an enlarged view of the structure A of FIG. 4 according to the present invention;

FIG. 6 is an enlarged view of the structure B of FIG. 4 according to the present invention;

fig. 7 is a second perspective view of the overall structure of the present invention;

FIG. 8 is an enlarged view of the structure of FIG. 7;

FIG. 9 is an enlarged view of the structure of FIG. 7;

fig. 10 is a third schematic perspective view of the overall structure of the present invention;

fig. 11 is an enlarged view of the structure of fig. 10 at E;

fig. 12 is a schematic perspective view of a structure of an embodiment of the sample application assembly of the present invention;

fig. 13 is a fourth schematic perspective view of the loading mechanism of the present invention;

fig. 14 is a first schematic perspective view of a second structure of the sample loading assembly according to the present invention;

FIG. 15 is a top view of a second structure of the sample loading assembly of the present invention;

fig. 16 is a second schematic perspective view of a second structure of the sample loading assembly according to the present invention;

fig. 17 is an enlarged view of the structure of fig. 16;

fig. 18 is a third schematic perspective view of a second structure of the sample loading assembly of the present invention.

Reference numerals:

100. a base station; 101. a reaction disc; 102. a placement groove; 103. a control component; a. a loading zone; b. a pyrolysis mixing zone; c. a cleaning zone; d. an elution purification zone;

200. a loading mechanism; 201. a hopper; 202. a support; 203. a sprocket; 204. a chain; 205. a baffle plate; 206. a loading channel; 207. a full cup photoelectric switch; 208. a positive and negative cup photoelectric switch; 209. a positive and negative cup assembly; 210. a blocking electromagnet; 211. releasing the electromagnet;

300. a sample adding mechanism; 301. a frame; 302. a sample application assembly; 3021. an X arm; 3022. a first motor; 3023. a Y arm; 3024. a second motor; 3025. a Z arm; 3026. a third motor; 3027. a sample needle; 302.1, a pipetting pump mounting rack; 302.2, a pipetting pump; 302.3, TIP header; 302.4, gear; 302.5, a rack; 302.6, placing a TIP head box; 302.7, discarding the channel; 303. a reagent sample application assembly; 3031. a first base; 3032. a rotating shaft; 3033. a reagent sample addition arm; 3034. a reagent needle; 3035. a fifth motor; 304. a rinsing station; 305. a sample placement box; 306. a lysate storage box; 307. a magnetic bead uniformly mixing component; 3071. a second base; 3072. a driven wheel; 3073. a driving wheel; 3074. a magnetic bead storage cylinder; 308. a second vibrating assembly;

400. a cleaning mechanism; 401. a fixed seat; 402. a fourth motor; 403. a movable frame; 404. cleaning the needle fixing plate; 405. cleaning the drawing needle; 406. a magnet disc; 407. cleaning the punching needle;

500. an elution mechanism; 501. a suction needle; 502. an eluent injection needle support frame; 503. injecting eluent into the liquid needle; 504. a first vibrating assembly; 505. a purification recovery box; 506. automatically dropping the waste cup assembly; 5061. a waste cup baffle; 5062. an electromagnet; 5063. an electromagnet fixing seat.

Detailed Description

The technical solutions in the embodiments of the present disclosure will be clearly and completely described below with reference to the drawings in the embodiments of the present disclosure, and it is obvious that the described embodiments are only a part of the embodiments of the present disclosure, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments disclosed herein without making any creative effort, shall fall within the protection scope of the present disclosure.

Referring to fig. 1-18, the present invention provides a technical solution: a nucleic acid extractor, comprising:

the device comprises a base table 100, a reaction disc 101 is rotatably connected on the base table 100, a placing groove 102 is formed in the reaction disc 101, and a water storage tank and a waste liquid box are further arranged on the base table 100 and are respectively used for supplying water and collecting waste liquid in the extraction operation; a loading mechanism 200 provided on the base 100 for automatically loading the cuvette into the placing groove 102 on the reaction disk 101; a sample adding mechanism 300 provided on the base 100 and configured to add a sample solution and a reagent into a cuvette mounted on the reaction disk 101; a cleaning mechanism 400, disposed on the base 100, for cleaning impurities in the sample solution mixed with the reagent in the cuvette; and an elution mechanism 500 provided on the base 100 for separating magnetic beads and nucleic acids from each other in the washed sample solution.

It should be noted that the whole device is powered by an external power supply, the control module 103 is disposed on the base 100, and the control module 103 is electrically connected to the loading mechanism 200, the sample adding mechanism 300, the cleaning mechanism 400 and the elution mechanism 500, so that the mechanisms are closely matched to extract nucleic acids.

Further, as shown in fig. 3, the reaction tray 101 is integrally divided into a loading area a, a cracking mixing area b, a cleaning area c and an elution and purification area d, a step motor is disposed at the bottom of the reaction tray 101, and is fixedly connected to the base 100, and the step motor is started to rotate a certain angle each time, so that the reaction tray 101 can be driven to move forward to a position of one placing groove 102.

The loading mechanism 200 comprises a hopper 201 arranged on the base 100, a bracket 202 connected inside the hopper 201, a chain wheel 203 rotatably connected on the bracket 202, a chain 204 movably connected on the chain wheel 203, a baffle 205 arranged on the chain 204, and a loading channel 206 arranged on the bracket 202, wherein the upper end opening of the loading channel 206 is communicated with the side wall of the bracket 202, the lower end opening is positioned right above the placing groove 102, so that reaction cups can be automatically loaded into the placing groove 102 from the chain 204, a full cup photoelectric switch 207 arranged on one side of the upper end of the loading channel 206, a positive and negative cup photoelectric switch 208 arranged on the other side of the loading channel 206 close to the upper end position, the full cup photoelectric switch 207 is arranged above the positive and negative cup photoelectric switch 208, a positive and negative cup assembly 209 arranged on the loading channel 206 close to the middle position, a blocking electromagnet 210 arranged on the side wall of the loading channel 206, a release electromagnet 211 arranged on the side wall of the loading channel 206, the output ends of the blocking electromagnet 210 and the release electromagnet 211 penetrate through one side of the loading channel 206, and the blocking electromagnet 210 and the release electromagnet 211 are arranged between the positive and negative cup photoelectric switch 207 and the full cup photoelectric switch 208.

It should be noted that the number of the chain wheels 203 is two, one side of one of the chain wheels 203 is provided with a sixth motor, and the sixth motor is started to drive the chain wheel 203 to rotate, so that the chain 204 can be driven to rotate.

Further, the baffle plates 205 are obliquely arranged on the chain 204, a conveying groove is formed between the front baffle plate 205 and the rear baffle plate 205, the reaction cups are placed in the conveying groove, and when the reaction cups are conveyed to the loading channel 206 by the chain 204, the reaction cups can fall into the loading channel 206 from the conveying groove.

Further, positive and negative cup subassembly 209 includes eighth motor and regulating part, and electric connection between positive and negative cup photoelectric switch 208 and the positive and negative cup subassembly 209, when positive and negative cup photoelectric switch 208 detected the reaction cup direction inconsistent, the eighth motor started to drive the regulating part and rotates, can adjust the reaction cup that gets into loading channel 206 into same direction then whereabouts.

Specifically, as shown in fig. 6-8, firstly, the reaction cups are placed in the conveying grooves formed on the chain 204, then the sixth motor is started to drive the sprocket 203 to rotate, the chain 204 can be driven to rotate to convey the reaction cups, when the chain 204 conveys the reaction cups to the loading channel 206, the reaction cups can fall into the loading channel 206 from the conveying grooves, the reaction cups fall to the blocking electromagnet 210 and stop, the full-cup photoelectric switch 207 detects the reaction cups, the reaction cups fall to the releasing electromagnet 211 after the blocking electromagnet 210 acts, the releasing electromagnet 211 works after the blocking electromagnet 210 finishes working, the reaction cups can fall into the positive and negative cup assembly 209 one by one, after the positive and negative cup assembly 209 finishes adjusting the direction of the reaction cups, the reaction cups can fall into the placing grooves 102, each time the reaction tray 101 moves forward by one placing groove 102, the chain 204 conveys one reaction cup into the loading channel 206, and the reaction cups fall into the placing groove 102, so that the reaction cups can be automatically loaded, the traditional reagent kit is prevented from being frequently taken and placed manually, and the labor intensity is reduced.

The sample adding mechanism 300 comprises a frame 301 arranged on the base station 100, a sample adding component 302 arranged on the frame 301, a sample placing box 305 arranged on the base station 100, a sample solution storing box 306 arranged on the base station 100 and a lysate mixing component 307 arranged on the base station 100.

It should be noted that, the base platform 100 is fixedly connected with the flushing station 304, flushing heads are obliquely arranged on two sides of the flushing station 304, one end of each flushing head is connected with a flushing pump through a guide pipe, the flushing pump is connected with the water storage tank through a guide pipe, the flushing pump is used for conveying the water storage tank into the flushing heads, the sample adding assembly 302 can be flushed after sample adding, and next operation is convenient to perform.

As shown in fig. 12, the first embodiment of the sample loading assembly 302 is as follows: the sample loading assembly 302 includes an X arm 3021 fixedly connected to the control assembly 103, a first motor 3022 fixedly connected to the X arm 3021, a Y arm 3023 movably connected to the X arm 3021, a second motor 3024 fixedly connected to the Y arm 3023, a Z arm 3025 movably connected to the Y arm 3023, a third motor 3026 fixedly connected to the Z arm 3025, and a sample needle 3027 provided on the Z arm 3025, wherein a first pump and a conduit are provided on the sample needle 3027, and a sample solution can be pumped into the sample needle 3027 by the first pump and then injected into the reaction cup, and after each injection, the sample solution needs to be washed at the washing station 304.

At this time, the output ends of the first motor 3022, the second motor 3024, and the third motor 3026 are respectively connected to a first transmission belt, a second transmission belt, and a third transmission belt, the first transmission belt is connected to the Y arm 3023 by a connection member, the second transmission belt is connected to the Z arm 3025 by a connection member, and when the first motor 3022 is started, the first transmission belt is driven to rotate, so that the Y arm 3023 can be driven to move in the X axis direction; when the second motor 3024 is started, the second driving belt is driven to rotate, so that the Z arm 3025 can move along the Y axis direction; when the third motor 3026 is activated, the third belt is rotated, and the sample needle 3027 can be moved in the Z-axis direction.

As shown in fig. 14-18, the second embodiment of the sample application assembly 302 is: the sample loading assembly 302 includes an X arm 3021 fixedly connected to the control assembly 103, a first motor 3022 fixedly connected to the X arm 3021, a Y arm 3023 movably connected to the X arm 3021, a second motor 3024 fixedly connected to the Y arm 3023, a Z arm 3025 movably connected to the Y arm 3023, a third motor 3026 fixedly connected to the Z arm 3025, a gear 302.4 provided on the third motor, and a pipetting pump mount 302.1 movably connected to the Z arm, wherein a rack 302.5 engaged with the gear 302.4 is provided on a side wall of the pipetting pump mount 302.1, a pipetting pump 302.2 provided on the pipetting pump mount 302.1, and a TIP head 302.3 provided on the pipetting pump 302.2.

At this time, the output ends of the first motor 3022 and the second motor 3024 are respectively connected with a first transmission belt and a second transmission belt, the first transmission belt is connected with the Y arm 3023 through a connecting piece, the second transmission belt is connected with the Z arm 3025 through a connecting piece, and when the first motor 3022 is started, the first transmission belt is driven to rotate, so that the Y arm 3023 can be driven to move along the X axis direction; when the second motor 3024 is started, the second driving belt is driven to rotate, so that the Z arm 3025 can move along the Y axis direction; when the third motor 3026 is activated, the gear 302.4 is driven to rotate in the forward or reverse direction, so that the pipetting pump mounting rack 302.1 drives the pipetting pump 302.2 and the TIP head 302.3 to move along the Z-axis direction.

Further, when the TIP head 302.3 is used to perform a sample loading operation, the pipette pump 302.2 does not need a tube and a washing station 304, so the washing station 304 can be replaced by a TIP head placing box 302.6, a new TIP head 302.3 can be replaced before each operation, and the base 100 is further provided with a discarding channel 302.7 for performing a centralized collection process on the discarded TIP heads 302.3.

Further, when sample addition is required, the X arm 3021, the Y3023 arm, and the Z arm 3025 are moved to move the pipetting pump mounting rack 302.1 to the TIP head placement box 302.6 to pick up the TIP head 302.3, and then moved to the sample placement box 305, at this time, the pipetting pump 302.2 is started to draw a sample, and then the pipetting pump 302.2 is further moved to the cuvette at the sample addition position to perform sample addition, after the sample addition is completed, the X arm 3021, the Y3023 arm, and the Z arm 3025 drive the pipetting pump 302.2 and the TIP head 302.3 to move to the discarding channel 302.7, and the pipetting pump 302.2 discards the TIP head 302.3, and when nucleic acid extraction is required, the X arm 3021, the Y3023 arm, and the Z arm 3025 are moved to drive the pipetting pump 302.2 to move a TIP head 302.3 to the elution product extraction position again, extract the nucleic acid, further purify the recovered sample, and then the TIP head 302.3 can be discarded again after the sample addition to the discarding channel 302.7.

On the other hand, as shown in fig. 10, the reagent sample feeding assembly 303 includes a first base 3031 fixedly connected to the base 100, a rotating shaft 3032 rotatably connected to the first base 3031, a reagent sample feeding arm 3033 connected to the rotating shaft 3032, and a reagent needle 3034 disposed on the reagent sample feeding arm 3033, a ninth motor is disposed below the first base 3031, the ninth motor is in transmission connection with the rotating shaft 3032 through a timing belt, so as to drive the reagent needle 3034 to move up and down for extraction and injection operations, a fifth motor 3035 is further disposed on one side of the first base 3031, and the fifth motor 3035 is in transmission connection with the rotating shaft 3032 through a fourth transmission belt, so as to drive the reagent sample feeding arm 3033 to deflect for reagent sample feeding operations.

Further, the reagent needle 3034 is connected with a fourth pump body and a conduit, and reagent solution can be extracted by the fourth pump body and then injected into the reaction cup.

In addition, as shown in fig. 11, magnetic bead blending subassembly 307 includes fixed connection second base 3071 on the base station 100, sets up seat 3072 is placed to the magnetic bead bottle on the second base 3071, the setting is in action wheel 3073 on the second base 3071, seat 3072 is placed to the magnetic bead bottle is connected on second base 3071 through following the rotation of driving wheel, the setting is in magnetic bead bottle 3074 on seat 3072 is placed to the magnetic bead bottle, fixedly connected with seventh motor on the action wheel 3073, start-up seventh motor and drive action wheel 3073 and rotate, then place seat 3072 through driving the magnetic bead bottle from the driving wheel and rotate, thereby drive magnetic bead bottle 3074 and rotate, be convenient for carry out the mixing to the magnetic bead, prevent the sediment magnetic bead, make at every turn extract magnetic bead quantity unanimously.

Further, be provided with second vibration subassembly 308 on the base station 100, second vibration subassembly 308 includes that heating plate, guide rail, vibrating motor, connecting piece constitute, is provided with heating band and temperature control switch on the heating plate, and the heating band is just the ohmic heating once started, makes the heating plate preferentially reach predetermined heating temperature, realizes the accurate heating to liquid in the reaction cup through the temperature control to the heating plate, guarantees that the high efficiency of nucleic acid is appeared.

Specifically, after the reaction cup is loaded, the reaction tray 101 drives the reaction cup to move forward, at this time, the sample needle 3027 can be inserted into the sample placing box 305 by starting the first motor 3022, the second motor 3024, and the third motor 3026 and matching with the first transmission belt, the second transmission belt, and the third transmission belt, and then the first pump body can be started to draw the sample solution into the sample needle 3027, and then move and inject the sample solution into the reaction cup at the sample loading position, and at the same time, the fifth motor 3035 is started, and the rotating shaft 3032 is driven to rotate by the fourth transmission belt, so that the reagent loading arm 3033 can be moved, and the reagent needle 3034 can be used to respectively draw the lysis solution and the magnetic beads, and then add the lysis solution and the magnetic beads into the reaction cup, then the reaction tray 101 can continue to rotate and drive the reaction cup to move forward, at this time, the second vibration assembly 308 is started to vibrate the liquid in the reaction cup in the whole lysis mixing region b, so that the reaction rate can be increased, and the nucleic acid can be precipitated quickly and efficiently.

The cleaning mechanism 400 comprises a fixed seat 401 fixedly connected to the base 100 and a movable frame 403 movably connected to the side wall of the fixed seat 401, a slide rail is arranged on the fixed seat 401, a slide block is movably connected in the slide rail, the movable frame 403 is fixed on the slide block, a cleaning needle fixing plate 404 is arranged on the movable frame 403, a cleaning drawing needle 405 is arranged on the cleaning needle fixing plate 404, and a cleaning punching needle 407 is arranged on the base 100.

In addition, the base 100 is provided with the magnetic disks 406 at the washing area c and the elution purification area d, and the magnetic disks 406 can attract the magnetic beads having nucleic acids adsorbed on the surfaces thereof to both sides of the reaction cup during washing, thereby preventing the nucleic acids and the magnetic beads from being drawn away during impurity suction.

It should be noted that the number of the cleaning drawing pins 405 is five, the number of the cleaning punching pins 407 is four, the cleaning drawing pins 405 and the cleaning punching pins 407 are sequentially arranged in a staggered manner, the cleaning drawing pins 405 are connected with a second pump body through silica gel hoses, and the second pump body is connected with a waste liquid box through the silica gel hoses; wash towards needle 407 through silica gel hose and third pump body connection, and the third pump body passes through silica gel hose and is connected with the storage water tank, and the quantity of the third pump body is two, and every two wash towards needle 407 and connect on a third pump body.

Further, be connected with fourth motor 402 on the fixing base 401, be provided with the slide rail on the fixing base 401, swing joint has the slider in the slide rail, adjustable shelf 403 is fixed on the slider, swing joint has the fifth drive belt on the fourth motor 402, fixed connection between adjustable shelf 403 and the fifth drive belt, forward or reverse rotation fourth motor 402 drives the fifth drive belt and rotates, can drive adjustable shelf 403 and reciprocate, thereby it takes out needle 405 downstream to drive in the reaction cup with the impurity suction waste liquid box.

Specifically, as shown in fig. 4-5, after the nucleic acid is precipitated, the reaction tray 101 continues to drive the reaction cup to move forward, when the reaction cup moves into the cleaning region c, the reaction cup moves below the cleaning probe 405, at this time, the fourth motor 402 is started to drive the fifth transmission belt to rotate, and the movable frame 403 can be driven to move downward, so as to drive the cleaning probe 405 to move downward into the reaction cup to pump the impurities into the waste liquid box, and then the reaction cup continues to move forward below the cleaning probe 407, and the third pump body is used to inject clean water into the reaction cup again to wash the reaction cup, so that the cleaning is performed repeatedly and continuously for multiple times, and the impurities except the nucleic acid and the magnetic beads can be cleaned.

Elution mechanism 500 is including setting up the suction needle 501 on wasing needle fixed plate 404, suction needle 501 also passes through the second pump body, the pipe is connected with the waste liquid box, utilize suction needle 501, can take out the water in the reaction cup after wasing, make things convenient for add the eluant in the follow-up step, eluant injection needle support frame 502 of setting on base station 100, eluant injection needle 503 of setting on eluant injection needle support frame 502, purification recovery box 505 of setting on base station 100, utilize magnet disc 406 can adsorb the magnetic bead with nucleic acid separation to the reaction cup both sides when retrieving the purification, conveniently extract nucleic acid from the reaction cup.

As shown in fig. 5, an eluent storage cylinder is provided on the base 100, a fifth pump is connected to one end of the eluent injection needle 503, the fifth pump is connected to the eluent storage cylinder through a hard tube, and the fifth pump is activated to inject the eluent into the reaction cup to elute and separate nucleic acids from magnetic beads.

Further, a first vibration component 504 is disposed on the base 100, and the first vibration component 504 is preferably a vibrator capable of vibrating the reaction cup rotated to a position right below the eluent injection needle 503, so as to accelerate the separation rate of the magnetic beads and the nucleic acids.

On the other hand, the base 100 is provided with an automatic waste cup dropping assembly 506, the magnet disc 406 is provided with an automatic waste cup dropping assembly 506, the automatic waste cup dropping assembly 506 is composed of a waste cup baffle 5061, an electromagnet 5062 and an electromagnet fixing seat 5063, when the nucleic acid in the reaction cup is completely extracted, the reaction cup is moved to a waste cup dropping position formed on the automatic waste cup dropping assembly 506, the electromagnet 5062 is electrified at the moment, the waste cup baffle 5061 is brought to move, the waste cup automatically drops into a waste cup recovery box, the electromagnet 5062 is powered off after the completion, and the waste cup baffle 5061 is reset.

Specifically, after impurities in the reaction cup are cleaned, the reaction disc 101 drives the reaction cup to move to a position below the suction needle 501, at this time, the second pump body is started to enable the suction needle 501 to pump excess water in the reaction cup into the waste liquid box, then the reaction cup continues to move forward to a position below the eluent injection needle 503, the fifth pump body is started to convey eluent from the eluent liquid storage cylinder to the eluent injection needle 503 through the conduit, then the eluent injection needle 503 is used to inject the eluent into the reaction cup, so that magnetic beads and nucleic acids are separated conveniently, after the nucleic acids are separated from the magnetic beads, the reaction cup continues to move in the elution and purification area d, at this time, the magnetic beads can be adsorbed to two sides of the reaction cup through the disc 406, then the cleaned sample loading assembly 302 is used to move to a proper position again, the extracted nucleic acids are pumped and moved to the recovery box 505, and subsequent purification treatment is facilitated.

It should be noted that, in this document, relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

The specific meaning of the above terms in the present invention can be understood according to specific situations by those of ordinary skill in the art. When an element is referred to as being "mounted to," "secured to," or "disposed on" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. As used herein, the terms "vertical," "horizontal," "upper," "lower," "left," "right," and the like are for purposes of illustration only and do not denote a single embodiment.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

In the description herein, references to the description of "one embodiment," "an example," "a specific example," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the disclosure. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

Claims (10)

1. A nucleic acid extractor, comprising:

the device comprises a base table (100), wherein a reaction disc (101) is connected to the base table (100) in a rotating mode, and a placing groove (102) is formed in the reaction disc (101);

a loading mechanism (200) which is arranged on the base (100) and is used for automatically loading the reaction cups into the placing grooves (102) on the reaction disc (101);

a sample addition mechanism (300) provided on the base (100) and configured to add a sample solution and a reagent to a cuvette mounted on the reaction disk (101);

a cleaning mechanism (400) which is arranged on the base station (100) and is used for cleaning impurities in the sample solution mixed with the reagent in the reaction cup;

and an elution mechanism (500) provided on the base (100) for separating the magnetic beads and the nucleic acids from each other in the washed sample solution.

2. The nucleic acid extractor of claim 1, wherein: the base station (100) is provided with a control component (103), and the control component (103) is electrically connected with the loading mechanism (200), the sample adding mechanism (300), the cleaning mechanism (400) and the eluting mechanism (500).

3. The nucleic acid extractor of claim 1, wherein: the loading mechanism (200) comprises a hopper (201) arranged on the base station (100), a support (202) connected inside the hopper (201), a chain wheel (203) rotatably connected to the support (202), a chain (204) movably connected to the chain wheel (203), a baffle (205) arranged on the chain (204), a loading channel (206) arranged on the support (202), a full cup photoelectric switch (207) arranged on one side of the upper end of the loading channel (206), a positive and negative cup photoelectric switch (208) arranged on the other side of the loading channel (206) close to the upper end position, wherein the full cup photoelectric switch (207) is arranged above the positive and negative cup photoelectric switch (208), a positive and negative cup component (209) arranged on the loading channel (206) close to the middle position, a blocking electromagnet (210) arranged on the side wall of the loading channel (206), and a releasing electromagnet (211) arranged on the side wall of the loading channel (206).

4. The nucleic acid extractor of claim 1, wherein: application of sample mechanism (300) is including setting up frame (301) on base station (100), sets up sample application of sample subassembly (302) on frame (301), set up and be in reagent application of sample subassembly (303) on base station (100), setting are in box (305), setting are placed to sample on base station (100) lysis solution on base station (100) is placed box (306), is set up bead mixing subassembly (307) on base station (100).

5. The nucleic acid extractor of claim 1, wherein: cleaning mechanism (400) is including fixed connection fixing base (401), swing joint on base station (100) adjustable shelf (403) on fixing base (401) lateral wall, setting are in cleaning needle fixed plate (404) on adjustable shelf (403), setting are in cleaning on the cleaning needle fixed plate (404) takes out needle (405), sets up washing towards needle (407) on base station (100).

6. The nucleic acid extractor of claim 5, wherein: the elution mechanism (500) comprises a suction needle (501) arranged on the cleaning needle fixing plate (404), an eluent liquid injection needle support frame (502) arranged on the base station (100), an eluent liquid injection needle (503) arranged on the eluent liquid injection needle support frame (502), a first vibration component (504) arranged on the base station (100) and a purification recovery box (505) arranged on the base station (100).

7. The nucleic acid extractor of claim 4, wherein: the sample adding assembly (302) comprises an X arm (3021) fixedly connected to the base station (100), a Y arm (3023) movably connected to the X arm (3021), a Z arm (3025) movably connected to the Y arm (3023), and a sample needle (3027) arranged on the Z arm (3025).

8. The nucleic acid extractor of claim 4, wherein: the sample adding assembly (302) comprises an X arm (3021) fixedly connected to the base (100), a Y arm (3023) movably connected to the X arm (3021), a Z arm (3025) movably connected to the Y arm (3023), a pipetting pump mounting rack (302.1) movably connected to the Z arm (3025), a pipetting pump (302.2) arranged on the pipetting pump mounting rack (302.1), and a TIP head (302.3) arranged on the pipetting pump (302.2).

9. The nucleic acid extractor of claim 4, wherein: the reagent sample adding assembly (303) comprises a first base (3031) fixedly connected to the base station (100), a rotating shaft (3032) rotatably connected to the first base (3031), a reagent sample adding arm (3033) connected to the rotating shaft (3032), and a reagent needle (3034) arranged on the reagent sample adding arm (3033).

10. The nucleic acid extractor of claim 4, wherein: bead mixing subassembly (307) is including fixed connection second base (3071), the setting on base station (100) is in bead bottle on second base (3071) is placed seat (3072), is set up action wheel (3073), the setting on second base (3071) are in bead bottle (3074) on placing seat (3072) are placed to the bead bottle.

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