CN112179738B - Novel cell pelleter - Google Patents
Novel cell pelleter Download PDFInfo
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- CN112179738B CN112179738B CN202011070333.3A CN202011070333A CN112179738B CN 112179738 B CN112179738 B CN 112179738B CN 202011070333 A CN202011070333 A CN 202011070333A CN 112179738 B CN112179738 B CN 112179738B
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- 230000007246 mechanism Effects 0.000 claims abstract description 77
- 238000004043 dyeing Methods 0.000 claims description 51
- 238000003860 storage Methods 0.000 claims description 47
- 239000002699 waste material Substances 0.000 claims description 38
- 238000005070 sampling Methods 0.000 claims description 29
- 239000007788 liquid Substances 0.000 claims description 25
- 238000003825 pressing Methods 0.000 claims description 22
- 238000005119 centrifugation Methods 0.000 claims description 14
- 238000004519 manufacturing process Methods 0.000 claims description 13
- 238000002347 injection Methods 0.000 claims description 9
- 239000007924 injection Substances 0.000 claims description 9
- 230000009471 action Effects 0.000 claims description 7
- 230000000712 assembly Effects 0.000 claims description 7
- 238000000429 assembly Methods 0.000 claims description 7
- 230000005484 gravity Effects 0.000 claims description 5
- 238000012546 transfer Methods 0.000 claims description 4
- 238000004064 recycling Methods 0.000 claims description 2
- 230000006835 compression Effects 0.000 claims 1
- 238000007906 compression Methods 0.000 claims 1
- 238000010186 staining Methods 0.000 description 18
- 230000001360 synchronised effect Effects 0.000 description 9
- 238000000034 method Methods 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 239000012192 staining solution Substances 0.000 description 4
- 230000005540 biological transmission Effects 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 230000010355 oscillation Effects 0.000 description 2
- 239000010902 straw Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000006727 cell loss Effects 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 231100000915 pathological change Toxicity 0.000 description 1
- 230000036285 pathological change Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
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- Health & Medical Sciences (AREA)
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- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
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- Pathology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a novel cell slide making machine which comprises a rack, a suction nozzle mechanism, a moving mechanism, a cell slide making table and a pressure pump set, wherein the suction nozzle mechanism is arranged at the top of the rack through the moving mechanism, the cell slide making table is arranged at the bottom of the rack, and the pressure pump set is arranged on the side wall of the rack and is connected with the suction nozzle mechanism. The cell slide making machine can finish the slide making of cells on the same workbench, effectively shortens the slide making time, saves the labor and improves the slide making efficiency.
Description
Technical Field
The invention relates to the technical field of cell pelleters, in particular to a novel cell pelleter.
Background
In the field of clinical medicine, the existence of pathological changes in a human body is often judged by observing cell slices of various tissues of the human body, and the cell slices are prepared by the following steps: sampling, centrifuging, smearing and dyeing, wherein each step is respectively carried out in a work area separated from each other, so that a cell sample needs to be transferred among a plurality of areas, and the time and the labor are consumed; at present, a cell slide manufacturing device which can complete the steps of temporary storage, centrifugation, smear and staining of a sample in sequence does not appear on the market.
Accordingly, the prior art is deficient and needs improvement.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a novel cell pelleter.
The technical scheme of the invention is as follows:
the utility model provides a novel cell pelleter, includes frame, suction nozzle mechanism, moving mechanism, cell pelleter manufacturing station and force pump group, suction nozzle mechanism passes through the moving mechanism and installs in the frame top, cell pelleter manufacturing station installs in the frame bottom, force pump group installs in the frame lateral wall and is connected with suction nozzle mechanism.
The suction nozzle mechanism comprises a suction nozzle component, the suction nozzle component comprises a group of large tube intake heads which can be sleeved and combined with a suction pipe for use, the package of a sample to be detected can be punctured after the large tube intake heads are sleeved with the suction pipe, injection operation and suction operation are carried out on the sample, injection objects in the injection operation pass through a channel of the large tube intake heads, and the suction objects in the suction operation are only contained in the suction pipe without passing through the channel of the large tube intake heads.
Preferably, the rack comprises a first base, a side vertical plate and a cover frame, the first base is horizontally arranged, a cell slide manufacturing table is arranged on the first base, the side vertical plate is arranged on the first base, a moving mechanism and a pressure pump set are arranged on the side vertical plate, and the cover frame is arranged on the side vertical plate.
Preferably, the suction nozzle mechanism comprises a fixing block, a dyeing tube fixing block and a plurality of suction pipes, the fixing block is mounted on the moving mechanism, the dyeing tube fixing block is mounted on the fixing block, the dyeing tube fixing block is mounted on the dyeing tube fixing block, the dyeing tube fixing block is provided with the suction pipes and connected to a pressure pump set, each suction pipe is connected with a pressure pump, and the large tube intake head is mounted on the fixing block and connected with the pressure pump set and located above the dyeing tube fixing block.
Preferably, the moving mechanism includes an X-axis moving structure, a Y-axis moving structure and a Z-axis moving structure, which are respectively used for controlling the nozzle mechanism to move in the X-axis direction, the Y-axis direction and the Z-axis direction.
Preferably, the cell slide manufacturing station comprises a first temporary storage device, a centrifugation device, a slide staining device, a second temporary storage device, a third temporary storage device, a first waste bucket, a second waste bucket, a first waste liquid groove and a second waste liquid groove; the first temporary storage device, the centrifugal device and the slide dyeing device are sequentially arranged on the second base from left to right, the first temporary storage device is used for temporarily storing a sample, the centrifugal device is used for realizing cell centrifugation, and the slide dyeing device is used for realizing slide dyeing; the second temporary storage device is arranged on the second base at the front side of the first temporary storage device and is used for temporarily storing the sampling tube; the third temporary storage device is arranged on the second base at the front side of the slide dyeing device and is used for temporarily storing the centrifuge tube; the first waste barrel is arranged on the second base between the second temporary storage device and the third temporary storage device and is used for recovering waste sampling tubes; the second waste barrel is arranged on the second base on the right side of the third temporary storage device and is used for recycling waste centrifuge tubes; the first waste liquid groove is arranged on the second base between the centrifugal device and the second temporary storage device and is used for recovering sample waste liquid; and the second waste liquid groove is arranged on the second base at the rear side of the slide dyeing device and is used for recovering dyeing waste liquid.
Preferably, the rotating mechanism further comprises a rotating shaft, a rotating shaft tube, a rotating shaft pressing ring, a fixing plate and a rotating plate; the rotating shaft is arranged in the centrifugal cavity, the rotating plate is arranged at the top of the rotating shaft, the rotating plate is uniformly provided with a plurality of mounting bins matched with the number of the centrifuge tube racks, and the centrifuge tube racks are rotatably mounted in the mounting bins; the rotating shaft pressing ring is arranged on the rotating shaft below the rotating plate; the rotating shaft tube is arranged on the rotating shaft below the rotating shaft pressing ring, and the rotating shaft tube is provided with the push-pull assembly; the fixing plate is arranged on the rotating shaft below the rotating shaft tube and is also connected with the shell to play a role of fixing and supporting; the bottom of the rotating shaft penetrates out of the shell downwards and is connected with the output end of the power mechanism;
the rotating plate is provided with a pressure pad above each centrifuge tube rack respectively, and plays a role in limiting the centrifuge tube racks; when in use, the pressure pad realizes the control of the swing direction and angle of the centrifugal tube rack in the centrifugal process.
Preferably, the push-pull assembly comprises two push-pull devices arranged on two opposite sides of the rotating shaft pipe, a push block connected with output ends of the two push-pull devices, and a push plate arranged on the push block; when the push plate is used, the push block pulls the push plate to move through the action of the push-pull device connected with the push block.
Preferably, the power mechanism comprises a servo motor provided with an encoder, a driving wheel, a synchronous belt and a driven wheel; the driven wheel is fixedly arranged at the bottom of the rotating shaft, the servo motor is arranged on one side of the driven wheel, the driving wheel is connected to the output end of the servo motor, and the synchronous belt is arranged on the driving wheel and the driven wheel; when the synchronous belt type electric power transmission device is used, the servo motor pulls the driving wheel connected with the servo motor to rotate, the driving wheel pulls the driven wheel to rotate through the synchronous belt, and therefore the rotating shaft connected with the driven wheel is pulled by the driven wheel to rotate around the axis of the rotating shaft; furthermore, the power mechanism also comprises two fastening devices; two tight setting device respectively in the both sides of hold-in range install in the below of casing, and two tight setting device's position is adjustable, is used for adjusting the elasticity of hold-in range.
Preferably, the slide staining apparatus comprises a plurality of slide staining assemblies;
each of the slide staining assemblies includes:
the dyeing bottle is hollow, the bottom of the dyeing bottle is provided with an annular bulge and a rubber ring, and two opposite sides of the annular bulge are respectively extended with a lug;
the mounting seat is arranged on the second base on the right side of the centrifugal device and used for placing a carrier plate;
the glass slide dyeing device comprises a carrier plate, a plurality of dyeing bottles and a plurality of dyeing pieces, wherein the carrier plate is provided with grooves which are arranged in an array manner and are used for accommodating glass slides, and two inner sides of each groove are provided with buckling positions for buckling annular raised bumps at the bottom of each dyeing bottle;
the needle-shaped suction nozzle is inserted into the bottom of the staining bottle and used for sucking staining solution on the slide;
wherein, the two sides of the carrier plate are provided with handles for transferring the carrier plate.
Preferably, the second temporary storage device comprises a second mounting frame arranged on the second base at the front side of the first mounting frame and a sampling pipe frame detachably arranged on the second mounting frame; evenly be equipped with a plurality of sample tube holes on the sample tube support, each sample tube hole all is used for keeping in the sampling tube, just be equipped with the handle on the left and right sides of sample tube support respectively, the sample tube support's of being convenient for transfer.
Preferably, the third temporary storage device comprises a mounting seat, a third mounting frame with the front side arranged on the second base and a temporary storage pipe frame arranged on the third mounting frame; the pipe support of keeping in is equipped with a plurality of pipe holes of keeping in, each the pipe hole of keeping in all is used for keeping in the centrifuging tube.
By adopting the technical scheme of the invention, the invention has the following beneficial effects:
1. the combined design of the invention realizes the cell slide of the steps of temporary storage of samples, centrifugation, smear and staining on the same workbench in sequence, effectively shortens the slide time, saves the labor and improves the slide efficiency;
2. the centrifugal tube is kept in an inclined state in the feeding process, so that liquid injected into the centrifugal tube can flow into the tube along the tube wall, the feeding is smooth, the liquid oscillation is not easy to cause, cells are effectively prevented from being dispersed in the upper layer liquid, the cell loss after centrifugation is effectively reduced, and the use experience is good;
3. the invention realizes the control of the swing direction and angle of the centrifugal tube in the centrifugal process, effectively avoids the damage of the centrifugal tube caused by collision, ensures the integrity of the centrifugal tube and has good centrifugal effect;
4. the encoder in the optimized scheme effectively ensures that the number of turns of the servo motor rotating during each operation is a fixed value, realizes the positioning function of the centrifuge tube rack, namely ensures that the centrifuge tube rack returns to the original position after each centrifugation, and realizes good positioning;
5. the application of the tightening device in the preferred scheme can effectively regulate and control the tightness of the synchronous belt, and ensure the transmission accuracy;
6. the application of pressure pad in the preferred scheme can guarantee that after the centrifugation, each centrifuge tube rack all is in the coplanar, when the centrifugation, does not harm each centrifuge tube rack when the centrifugation, and the face leanin for the synchronous slope of centrifuging tube, the bottom that is in the centrifuging tube behind the cell centrifugation.
Drawings
FIG. 1 is a perspective view of the present invention without the cell slide manufacturing stage mechanism;
FIG. 2 is a schematic view of the nozzle mechanism of the present invention;
FIG. 3 is a perspective view of the cell slide manufacturing stand according to the present invention;
FIG. 4 is an enlarged view taken at A in FIG. 3;
FIG. 5 is a perspective view of the centrifuge of the present invention;
FIG. 6 is a perspective view of the centrifuge with the housing and the power mechanism removed;
FIG. 7 is a side view of the centrifuge with the housing and power mechanism removed in accordance with the present invention;
FIG. 8 is a cross-sectional view taken at view A-A of FIG. 7;
FIG. 9 is a perspective view of the centrifuge apparatus of the present invention with the housing, centrifuge tube rack, centrifuge tube and power mechanism removed;
fig. 10 is a perspective view of a power mechanism in the centrifugal device according to the present invention.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings and examples. It is to be understood that the specific embodiments described herein are merely illustrative of the invention and are not limiting of the invention. It should be further noted that, for the convenience of description, only some of the structures related to the present invention are shown in the drawings, not all of the structures.
In the description of the present invention, unless expressly stated or limited otherwise, the terms "connected," "connected," and "fixed" are to be construed broadly, e.g., as meaning permanently connected, removably connected, or integral to one another; can be mechanically or electrically connected; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
In the present invention, unless otherwise expressly stated or limited, "above" or "below" a first feature means that the first and second features are in direct contact, or that the first and second features are not in direct contact but are in contact with each other via another feature therebetween. Also, the first feature being "on," "above" and "over" the second feature includes the first feature being directly on and obliquely above the second feature, or merely indicating that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature includes the first feature being directly under and obliquely below the second feature, or simply meaning that the first feature is at a lesser elevation than the second feature.
In the description of the present embodiment, the terms "upper", "lower", "left", "right", "front", "rear", and the like are used in the orientations and positional relationships shown in the drawings only for convenience of description and simplicity of operation, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus should not be construed as limiting the present invention. Furthermore, the terms "first", "second", and "third" are used only for descriptive purposes and are not intended to have any special meaning.
Referring to fig. 1 to 10, the invention provides a novel cell slide making machine, which comprises a frame 1, a suction nozzle mechanism 2, a moving mechanism 3, a cell slide making table 4 and a pressure pump set 5, wherein the suction nozzle mechanism 2 is installed at the top of the frame 1 through the moving mechanism 3, the cell slide making table is installed at the bottom of the frame 1, and the pressure pump set 5 is installed on the side wall of the frame and connected with the suction nozzle mechanism 2.
The suction nozzle mechanism comprises a suction nozzle component, the suction nozzle component comprises a group of large tube intake heads which can be sleeved and combined with a suction pipe for use, the package of a sample to be detected can be punctured after the large tube intake heads are sleeved with the suction pipe, injection operation and suction operation are carried out on the sample, injection objects in the injection operation pass through a channel of the large tube intake heads, and the suction objects in the suction operation are only contained in the suction pipe without passing through the channel of the large tube intake heads.
The frame 1 comprises a first base 11, a side vertical plate 12 and a cover frame 13, wherein the first base 11 is horizontally placed and is provided with a cell slide manufacturing platform 4, the side vertical plate 12 is installed on the first base 11 and is provided with a moving mechanism 3 and a pressure pump set 5, and the cover frame 13 is installed on the side vertical plate 12 and is used for protecting a suction nozzle mechanism and the moving mechanism.
The suction nozzle mechanism 2 comprises a fixing block 21, a dyeing tube fixing plate 22, a dyeing tube fixing block 23 and a plurality of suction pipes 24, wherein the fixing block 21 is installed on the moving mechanism 3, the dyeing tube fixing plate 22 is installed on the fixing block 21 through a guide post, the dyeing tube fixing block 23 is installed on the dyeing tube fixing plate 22, the dyeing tube fixing block 23 is provided with the suction pipes 24 and is connected to a pressure pump set 5, each suction pipe is connected with a pressure pump, a large tube intake head 25 is installed on the fixing block 21 and is connected with the pressure pump set 5, and the large tube intake head is located above the dyeing tube fixing plate 22. The plurality of straws comprise a plurality of dyeing needle tubes and a waste liquid straw, wherein the number of the dyeing needle tubes is generally 6, and the number of the large tube taking heads is 3.
The moving mechanism 3 comprises an X-axis moving structure, a Y-axis moving structure and a Z-axis moving structure, and is respectively used for controlling the suction nozzle mechanism to move in the X-axis direction, the Y-axis direction and the Z-axis direction. The X-axis moving structure is mounted at the top of the side vertical plate of the rack, the Y-axis moving structure is mounted on the X-axis moving structure, and the Z-axis moving structure is mounted on the Y-axis moving structure and connected with the fixed block of the suction nozzle mechanism.
The cell slide manufacturing station comprises a second base 41, a first temporary storage device, a centrifugal device, a slide staining device, a second temporary storage device, a third temporary storage device, a first waste barrel 42, a second waste barrel 43, a first waste liquid groove 44 and a second waste liquid groove 45; the first temporary storage device, the centrifugal device and the slide dyeing device are sequentially arranged on the second base 41 from left to right, the first temporary storage device is used for temporarily storing a sample, the centrifugal device is used for realizing cell centrifugation, and the slide dyeing device is used for realizing slide dyeing; the second temporary storage device is arranged on the second base 41 at the front side of the first temporary storage device and is used for temporarily storing the sampling tube; the third temporary storage device is arranged on the second base 41 at the front side of the slide dyeing device and is used for temporarily storing the centrifuge tube; the first waste bucket 42 is arranged on the second base 41 between the second temporary storage device and the third temporary storage device and is used for recovering waste sampling tubes; the second waste barrel 43 is mounted on the second base 41 at the right side of the third temporary storage device and is used for recovering waste centrifuge tubes; the first waste liquid tank 44 is arranged on the second base 41 between the centrifugal device and the second temporary storage device and is used for recovering sample waste liquid; the second waste liquid tank 45 is mounted on the second base 41 at the rear side of the slide dyeing apparatus, and is used for recovering dyeing waste liquid.
In this embodiment, the first buffer device includes a first mounting rack 46 disposed on the second base 41, a sample tube rack 47 detachably disposed on the first mounting rack 46, and a pressing plate 48 rotatably disposed on the first mounting rack 46 above the sample tube rack 47; a plurality of sample tube holes are uniformly formed in the sample tube frame 47, each sample tube hole is used for placing a sample bottle containing a sample, and handles are respectively arranged on the left side and the right side of the sample tube frame 47, so that the sample tube frame 47 can be conveniently transferred; and sampling holes are formed in the positions, corresponding to the sample tube holes, of the pressing plate 48 and used for matching with a sample moving device to realize positioning sampling.
Furthermore, the first mounting bracket 46 is provided with limiting devices 49 on the left and right sides of the pressing plate 48, each limiting device 49 is composed of a knob fixing block mounted on the first mounting bracket 46 and a knob rotatably mounted on the knob fixing block, and the pressing plate 48 is pressed or released by twisting the knob during use, so that the limiting operation of the pressing plate 48 is realized.
In this embodiment, the centrifuge device includes a housing 410, a power mechanism, a rotating mechanism, and a plurality of centrifuge tube racks 411; the shell 410 is arranged on the second base 41 on the right side of the first mounting frame 46, a centrifugal cavity is arranged in the shell 410, the rotating mechanism is arranged in the centrifugal cavity, a plurality of centrifuge tube racks 411 are rotatably arranged on the top of the rotating mechanism, a plurality of centrifuge tube holes are uniformly arranged on each centrifuge tube rack 411, each centrifuge tube hole is used for placing a centrifuge tube, and the bottom of the rotating mechanism penetrates out of the shell 410 downwards; the power mechanism is arranged below the shell 410, and the output end of the power mechanism is connected with the end part of the rotating mechanism, which is positioned outside the shell 410; the rotating mechanism comprises a push-pull assembly, a push-pull plate 412 is arranged on the inner edge of each centrifuge tube rack 411, and the push-pull assembly is used for pushing the push-pull plate 412; during use, one of the push-pull pieces 412 is pushed through the action of the push-pull assembly, so that the push-pull pieces 412 can drive the centrifuge tube rack 411 connected with the push-pull pieces to incline towards the inner side of the rotating mechanism, liquid injected into the centrifuge tube can slowly flow into the centrifuge tube along the tube wall of the centrifuge tube, feeding is smooth, liquid oscillation is not easily caused, cells are prevented from being dispersed in each liquid layer, the loss of the centrifuged cells is reduced, and the practical use experience is good.
The rotating mechanism further comprises a rotating shaft 413, a rotating shaft pipe 414, a rotating shaft pressing ring 415, a fixing plate 416 and a rotating plate 417; the rotating shaft 413 is arranged in the centrifugal cavity, the rotating plate 417 is arranged at the top of the rotating shaft 413, the rotating plate 417 is uniformly provided with mounting bins matched with the centrifuge tube racks 411 in number, and each mounting bin is rotatably provided with the centrifuge tube racks 411; the rotating shaft pressing ring 415 is arranged on the rotating shaft 413 below the rotating plate 417; the rotating shaft tube 414 is arranged on the rotating shaft 413 below the rotating shaft pressing ring 415, and the pushing and pulling assembly is arranged on the rotating shaft tube 414; the fixing plate 416 is disposed on the rotating shaft 413 below the rotating shaft tube 414, and the fixing plate 416 is further connected to the housing 410 to function as a fixing support; the bottom of the rotating shaft 413 passes through the housing 410 downward and is connected with the output end of the power mechanism.
The rotating plate 417 is provided with a pressure pad 418 above each centrifuge tube rack 411, so as to limit the centrifuge tube racks 411; pass through during the use the pressure pad 418 realizes the control to centrifuge tube rack 411 swing direction and angle in centrifugal process, effectively avoids bumping between the centrifuging tube on the centrifuge tube rack 411 of two relative settings, ensures the integrality of centrifuging tube.
The push-pull assembly comprises two push-pull devices 419 arranged on two opposite sides of the rotating shaft tube 414, a push block 420 connected with the output ends of the two push-pull devices 419, and a push plate 421 arranged on the push block 420; when in use, the push block 420 pulls the push plate 421 to move through the action of the push-pull device 419 connected with the push block, so that the push plate 421 can push and push the pull tab 412; specifically, the push-pull device 419 is a push-pull electromagnet.
The power mechanism comprises a servo motor 422 provided with an encoder, a driving wheel 423, a synchronous belt 424 and a driven wheel 425; the driven wheel 425 is fixedly arranged at the bottom of the rotating shaft 413, the servo motor 422 is arranged at one side of the driven wheel 425, the driving wheel 423 is connected to the output end of the servo motor 422, and the synchronous belt 424 is arranged on the driving wheel 423 and the driven wheel 425; when in use, the servo motor 422 pulls the driving wheel 423 connected with the servo motor to rotate, the driving wheel 423 pulls the driven wheel 425 to rotate through the synchronous belt 424, and therefore the driven wheel 425 pulls the rotating shaft 413 connected with the driven wheel to rotate around the axis of the driven wheel 425; further, the power mechanism further comprises two fastening devices 426; two the tight setting device 426 respectively in the both sides of hold-in range 424 install in the below of casing 410, and two the position of tight setting device 426 is adjustable, is used for adjusting hold-in range 424's elasticity has effectively ensured driven precision.
It is worth mentioning that the application of the encoder effectively ensures that the number of turns of the servo motor 422 during each working rotation is a fixed value, thereby ensuring that the initial position of each centrifuge tube rack 411 is reset after the centrifugal processing is completed, and realizing the positioning function of the centrifuge tube rack 411.
The outer edge of the bottom of each centrifuge tube rack 411 is provided with a gravity pressing block 427, and when the centrifugation work is completed, each centrifuge tube rack 411 can automatically reset under the gravity action of the gravity pressing block 427;
a cover plate accommodating groove is formed in the shell 410, and a cover plate 428 is arranged on the cover plate accommodating groove; the cover plate 428 is provided with a grabbing groove 429 for grabbing the cover plate 428; the cover plate 428 is further provided with an observation window 430 at one side of the grabbing groove 429, so that an operator can observe the working state in the centrifugal chamber conveniently.
The quantity of centrifuge tube rack 411 is four, and each evenly be equipped with twelve pipe holes on the centrifuge tube rack 411, it is corresponding, the quantity of installation storehouse also is four.
In the present embodiment, the slide staining apparatus includes several slide staining assemblies;
each of the slide staining assemblies includes:
the dyeing bottle is hollow, the bottom of the dyeing bottle is provided with an annular bulge and a rubber ring, and two opposite sides of the annular bulge are respectively extended with a lug;
a mounting seat 431 disposed on the second base 41 at the right side of the centrifuge for receiving a carrier plate 432;
the carrier plate 432 is provided with grooves 433 arranged in an array, the grooves 433 are used for accommodating slides, and two inner sides of each groove 433 are provided with buckling positions 434 for buckling annular raised bumps at the bottom of the dyeing bottle;
the needle-shaped suction nozzle is inserted into the bottom of the staining bottle and used for sucking staining solution on the slide;
wherein, handles 435 are arranged on two sides of the carrier plate 432 for transferring the carrier plate 432;
preferably, the number of slide staining assemblies is two.
In this embodiment, the second buffer device comprises a second mounting rack 436 disposed on the second base 41 at the front side of the first mounting rack 46 and a sampling tube rack 437 detachably disposed on the second mounting rack 436; evenly be equipped with a plurality of sample tube holes on the sample tube support 437, each sample tube hole all is used for laying the sampling tube to the realization is to the temporary storage of sampling tube, just be equipped with the handle on the left and right sides of sample tube support 437 respectively, the sample tube support 437's of being convenient for transfer.
In this embodiment, the third buffer device comprises a mounting base 431, a third mounting rack 438 arranged on the front side of the second base 41, and a buffer pipe rack 439 arranged on the third mounting rack 438; be equipped with a plurality of temporary storage tube holes on the pipe support 439 of keeping in, each the temporary storage tube hole all is used for laying the centrifuging tube to the realization is to the temporary storage of centrifuging tube.
The working principle of the invention is as follows: firstly, the cover plate 428 is opened, then the centrifuge tube temporarily stored on the temporary storage tube rack 439 is placed in the centrifuge tube hole of the centrifuge tube rack 411, and after the centrifuge tube is placed, the external sample moving device is inserted into the sampling tube, so that the combination of the sample moving device and the sampling tube is completed; then, the sample moving device is used for driving the sampling tube to move to the upper part of the sample tube rack 47, and the sampling tube is inserted into the sample bottle on the sample tube rack 47 for sampling; then, the sample moving device is used for driving the sampling tube to move above the centrifuge tube rack 411, and the sampling tube is inserted into the centrifuge tube on the centrifuge tube rack 411, so that the sample in the sampling tube can be injected into the centrifuge tube; then, the used sampling tube on the sample moving device is discarded into the first waste material barrel 42, the sample moving device is replaced by a new sampling tube, and the above operations of sampling and injecting samples are repeated until all the centrifuge tubes are injected with samples, at this time, the cover plate 428 can be covered; starting the servo motor 422, rotating the centrifuge tube rack 411 under the driving of the servo motor 422, and rotating the centrifuge tube along with the rotation of the centrifuge tube rack 411; the servo motor 422 rotates to the preset number of turns of the encoder and stops to finish the centrifugal treatment of the centrifugal tube, and at the moment, the cover plate 428 is opened and the centrifugal tube is taken out in sequence; then, the cell sample in the centrifugal tube is transferred to a slide, the slide is placed in the groove 433, the staining bottle is inserted into the groove 433, the projection is clamped into the clamping position 434 of the groove 433 in a rotating mode, the rubber ring seals the space between the staining bottle and the slide, so that staining solution can be dripped into the slide, after sufficient staining is conducted, redundant staining solution is sucked away through the needle-shaped suction nozzle, and after multiple times of staining (staining with different colors), cell slide can be obtained.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the scope of the present invention, and all modifications and equivalents of the present invention, which are made by the contents of the present specification and the accompanying drawings, or directly/indirectly applied to other related technical fields, are included in the scope of the present invention.
Claims (7)
1. A cell pelleter, its characterized in that: the cell slide production device comprises a rack, a suction nozzle mechanism, a moving mechanism, a cell slide production platform and a pressure pump set, wherein the suction nozzle mechanism is arranged at the top of the rack through the moving mechanism, the cell slide production platform is arranged at the bottom of the rack, and the pressure pump set is arranged on the side wall of the rack and connected with the suction nozzle mechanism;
the cell slide making machine comprises a rack, a cell slide making mechanism, a cell slide making;
the cell slide manufacturing platform comprises a second base, a first temporary storage device, a centrifugal device, a slide dyeing device, a second temporary storage device, a third temporary storage device, a first waste barrel, a second waste barrel, a first waste liquid groove and a second waste liquid groove; the first temporary storage device, the centrifugal device and the slide dyeing device are sequentially arranged on the second base from left to right, the first temporary storage device is used for temporarily storing a sample, the centrifugal device is used for realizing cell centrifugation, and the slide dyeing device is used for realizing slide dyeing; the second temporary storage device is arranged on the second base at the front side of the first temporary storage device and is used for temporarily storing the sampling tube; the third temporary storage device is arranged on the second base at the front side of the slide dyeing device and is used for temporarily storing the centrifuge tube; the first waste barrel is arranged on the second base between the second temporary storage device and the third temporary storage device and is used for recovering waste sampling tubes; the second waste barrel is arranged on the second base on the right side of the third temporary storage device and is used for recycling waste centrifuge tubes; the first waste liquid groove is arranged on the second base between the centrifugal device and the second temporary storage device and is used for recovering sample waste liquid; the second waste liquid groove is arranged on the second base at the rear side of the slide dyeing device and is used for recovering dyeing waste liquid;
the first temporary storage device comprises a first mounting frame arranged on the second base, a sample tube rack detachably arranged on the first mounting frame, and a pressing plate rotatably arranged on the first mounting frame above the sample tube rack; the sample tube rack is provided with a plurality of sample tube holes, each sample tube hole is used for placing a sample bottle containing the sample, and the left side and the right side of the sample tube rack are respectively provided with a handle, so that the sample tube rack is convenient to transfer; sampling holes are formed in the positions, corresponding to the sample tube holes, of the pressing plate and used for being matched with a sample moving device to achieve positioning sampling; the first mounting frame is provided with limiting devices on the left side and the right side of the pressing plate respectively, and each limiting device consists of a knob fixing block arranged on the first mounting frame and a knob rotatably arranged on the knob fixing block; when in use, the knob is twisted to realize the operation of pressing or releasing the pressing plate; the centrifugal device comprises a shell, a power mechanism, a rotating mechanism and a plurality of centrifugal pipe frames; the shell is positioned on the right side of the first mounting frame and is arranged on the second base, a centrifugal cavity is formed in the shell, the rotating mechanism is arranged in the centrifugal cavity, a plurality of centrifuge tube frames are rotatably arranged on the top of the rotating mechanism, a plurality of centrifuge tube holes are uniformly formed in each centrifuge tube frame, each centrifuge tube hole is used for placing a centrifuge tube, and the bottom of the rotating mechanism penetrates out of the shell downwards; the power mechanism is arranged below the shell, and the output end of the power mechanism is connected with the end part of the rotating mechanism, which is positioned outside the shell;
the rotating mechanism comprises push-pull assemblies, push-pull pieces are arranged on the inner edge of each centrifuge tube rack, and the push-pull assemblies are used for pushing the push-pull pieces; when the centrifugal tube pushing device is used, the operation of pushing one of the push-pull sheets is realized through the action of the push-pull assembly, so that the push-pull sheet can drive the centrifugal tube rack connected with the push-pull sheet to incline towards the inner side of the rotating mechanism;
the rotating mechanism also comprises a rotating shaft, a rotating shaft pipe, a rotating shaft compression ring, a fixed plate and a rotating plate; the rotating shaft is arranged in the centrifugal cavity, the rotating plate is arranged at the top of the rotating shaft, the rotating plate is uniformly provided with a plurality of mounting bins matched with the number of the centrifuge tube racks, and the centrifuge tube racks are rotatably mounted in the mounting bins; the rotating shaft pressing ring is arranged on the rotating shaft below the rotating plate; the rotating shaft tube is arranged on the rotating shaft below the rotating shaft pressing ring, and the rotating shaft tube is provided with the push-pull assembly; the fixing plate is arranged on the rotating shaft below the rotating shaft tube and is also connected with the shell to play a role of fixing and supporting; the bottom of the rotating shaft penetrates out of the shell downwards and is connected with the output end of the power mechanism;
the push-pull assembly comprises two push-pull devices arranged on two opposite sides of the rotating shaft pipe, a push block connected with the output ends of the two push-pull devices, and a push plate arranged on the push block; when the push plate is used, the push block pulls the push plate to move through the action of the push-pull device connected with the push block, so that the push plate can push and push the pull piece.
2. A cell pelleter as claimed in claim 1, in which: the suction nozzle mechanism comprises a suction nozzle component, the suction nozzle component comprises a group of large tube intake heads which can be sleeved and combined with a suction pipe for use, the package of a sample to be detected can be punctured after the large tube intake heads are sleeved with the suction pipe, injection operation and suction operation are carried out on the sample, injection objects in the injection operation pass through a channel of the large tube intake heads, and the suction objects in the suction operation are only contained in the suction pipe without passing through the channel of the large tube intake heads.
3. A cell pelleter as claimed in claim 2, in which: the suction nozzle mechanism comprises a fixing block, a dyeing tube fixing plate, a dyeing tube fixing block, a plurality of suction tubes and a large tube sucking head, wherein the fixing block is installed on the moving mechanism, the dyeing tube fixing plate is installed on the fixing block, the dyeing tube fixing block is installed on the dyeing tube fixing plate, the dyeing tube fixing block is provided with the suction tubes and connected to a pressure pump set, each suction tube is connected with a pressure pump, the large tube sucking head is installed on the fixing block and connected with the pressure pump set, and the large tube sucking head is located above the dyeing tube fixing plate.
4. A cell pelleter as claimed in claim 2, in which: the moving mechanism comprises an X-axis moving structure, a Y-axis moving structure and a Z-axis moving structure which are respectively used for controlling the suction nozzle mechanism to move in the directions of the X-axis, the Y-axis and the Z-axis.
5. A cell pelleter as claimed in claim 1, in which: the outer edge of the bottom of each centrifuge tube rack is provided with a gravity pressing block, and each centrifuge tube rack can automatically reset under the action of the gravity pressing block when the centrifugation work is finished; the shell is provided with a cover plate accommodating groove, and a cover plate is arranged on the cover plate accommodating groove; the cover plate is provided with a grabbing groove for grabbing the cover plate; the apron in snatch groove one side and still be equipped with the observation window, the operating condition of being convenient for operator observation centrifugal chamber.
6. The cell pelleter of claim 1, wherein the second staging device includes a second mounting bracket mounted to the second base at a front side of the first mounting bracket and a sampling tube holder detachably mounted to the second mounting bracket; evenly be equipped with a plurality of sample tube holes on the sample tube support, each sample tube hole all is used for keeping in the sampling tube, just be equipped with the handle on the left and right sides of sample tube support respectively, the sample tube support's of being convenient for transfer.
7. The cell pelleter of claim 1, wherein the third buffer device comprises a mounting base, a third mounting frame with a front side disposed on the second base, and a buffer tube rack disposed on the third mounting frame; the pipe support of keeping in is equipped with a plurality of pipe holes of keeping in, each the pipe hole of keeping in all is used for keeping in the centrifuging tube.
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| CN202011070333.3A CN112179738B (en) | 2020-09-30 | 2020-09-30 | Novel cell pelleter |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011070333.3A CN112179738B (en) | 2020-09-30 | 2020-09-30 | Novel cell pelleter |
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| CN112179738A CN112179738A (en) | 2021-01-05 |
| CN112179738B true CN112179738B (en) | 2021-07-13 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113267396B (en) * | 2021-07-16 | 2021-09-28 | 易普森生物科技(深圳)有限公司 | Cell film-making dyeing machine |
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