CN202362187U - Liquid-based cell slide preparation and dyeing machine based on injection pipetting principle - Google Patents
Liquid-based cell slide preparation and dyeing machine based on injection pipetting principle Download PDFInfo
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- CN202362187U CN202362187U CN201120430044XU CN201120430044U CN202362187U CN 202362187 U CN202362187 U CN 202362187U CN 201120430044X U CN201120430044X U CN 201120430044XU CN 201120430044 U CN201120430044 U CN 201120430044U CN 202362187 U CN202362187 U CN 202362187U
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- 238000004043 dyeing Methods 0.000 title claims abstract description 40
- 238000002347 injection Methods 0.000 title claims abstract description 11
- 239000007924 injection Substances 0.000 title claims abstract description 11
- 238000002360 preparation method Methods 0.000 title abstract 6
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 21
- 238000004062 sedimentation Methods 0.000 claims abstract description 13
- 238000001914 filtration Methods 0.000 claims abstract description 7
- 210000000270 basal cell Anatomy 0.000 claims description 22
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- 238000002386 leaching Methods 0.000 claims description 3
- 229920001778 nylon Polymers 0.000 claims description 3
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Abstract
The utility model relates to a liquid-based cell slide preparation and dyeing machine based on an injection pipetting principle. The liquid-based cell slide preparation and dyeing machine is characterized by comprising a machine base, a machine body, a workbench, a horizontal sliding rail, a moving arm, a horizontal motor moving device, a longitudinal motor moving device and a microcomputer moving control system, wherein four groups of slide preparation and dyeing units and a kit are arranged on the workbench; and the machine can automatically prepare and dye 24 samples at one time. Complicated liquid-based cytologic test (LCT) proportion liquid gradient centrifugation steps and a micro tube type liquid extraction system are eliminated, and a vacuum negative pressure extraction process and a microhole filtration film positive pressure cell transferring process of a temperature cycle test are eliminated. A pipetting injector is used for extracting, uniformly mixing and scattering cell samples and transferring sample reagents according to functions of precisely absorbing liquid quantitatively and injecting the liquid. Cells in a sedimentation slide preparation bin naturally sedimentate on an adhering glass slide under the gravity and are dyed automatically. The utility model has the characteristics that the liquid-based cell slide preparation and dyeing machine is easy to operate and relatively low in cost and can quickly prepare a liquid-based cell slide with high quality.
Description
Technical field
The utility model relates to and a kind ofly uses injection to move the liquid basal cell film-making overflow dyeing machine of liquid principle; Specifically a kind of simple efficient, film-making high-quality and high liquid based cytology film-making and the dyeing integration instrument and equipment of automaticity are applied in clinical medicine pathology, cytology check and biological study field.
Background technology
The traditional-handwork smear technique has almost been continued to use last 100 years unalterablely.The eighties is noticed the two big reasons that mainly contain of false negative case in the self-examination to the traditional-handwork smear technique.At first be to have cell more than 80% still to remain on the sampler in the smear process and thrown away; Next is the smear poor quality, and the abnormal cell on the microslide possibly overlap each other or covered and be difficult to discern by inflammatory cell, red blood cell; Smear also possibly diagnosed because of factor affecting such as fixing untimely, cell dryings.Problem to the existence of traditional-handwork smear; Be accompanied by the high speed development of computer information technology and automated control technology; From the later stage 1980s; Sample collection and tabletting technology that people's pair cell is learned constantly improve, and a series of liquid based cytology film-makings are arisen at the historic moment with the dyeing new technology.At present, mainly be that following two quasi-instruments are representative on the market.
The automatic liquid basal cell pelleter of film pipe filtering type is commonly called as TCT.Its uses filtering membrane tube high speed rotating to stir cell, relies on the negative pressure suction to preserve the liquid entrapped cell to the miillpore filter of film pipe, shifts cell on the filter membrane to microslide through malleation again.The mechanical factor of filtering membrane tube high speed rotating may cause destruction to a certain degree by pair cell; The miillpore filter of consumptive material assembly needs energetic ion or isotope technology to produce micropore; This just caused should technology adapted consumptive material expensive, satisfied inadequately to the production effect of courage and uprightness or mucus sample.In addition, this kind equipment can only be used for smear to be made, and can not realize the robotization dyeing of smear.
Natural subsidence formula liquid based cytology pelleter is commonly called as LCT.The cell collection of natural subsidence formula LCT is roughly similar with TCT with the preservation process, because whole film-making process adopts the specific gravity liquid gradient centrifugation twice, and then carries out film-making and dyeing, makes whole technique process redundant and complicated.And, to operating personnel's experience require high and labour intensity greatly, defectives such as consumptive material assembly complicacy and device fabrication cost height.Therefore, the liquid based cytology film-making dyeing integration equipment that a kind of simple efficient, film-making high-quality of invention and automaticity are high is very necessary concerning the application of the development of current cell pathology subject and clinical cytology inspection.
The utility model content
The technical matters that the utility model solved is to provide a kind of and uses injection to move the liquid based cytology film-making of liquid principle and the robot of dyeing integration; Can be as the liquid based cytology film-making dyeing of quick and high-quality, to solve the shortcomings and deficiencies in the above-mentioned background technology.
The technical matters that the utility model solved adopts following technical scheme to realize:
The feature structure of the utility model liquid basal cell film-making overflow dyeing machine comprises machine base; Worktable; Sample moves liquid syringe and moves the liquid syringe seat; Cell-preservation liquid bottle and preservation liquid bottle seat; The film-making plate of may command upset; Reagent moves liquid syringe and moves the liquid syringe seat; Kit; The micro computer kinetic control system; Fuselage; Kit has port lid; Forearm; Horizontal slide rail; Movement arm; Horizontal motor movement device; Horizontal motor; Motor telecontrol equipment up and down; Move liquid syringe operation paw; Top board and side arm.
The fuselage and the forearm of the utility model liquid basal cell film-making overflow dyeing machine support one group of horizontal slide rail; Movement arm is across between two tracks of horizontal slide rail, and horizontal motor movement device and motor telecontrol equipment and move liquid syringe operation paw up and down are housed on the movement arm.
Four groups of film-makings dyeing unit are installed on the worktable of the utility model liquid basal cell film-making overflow dyeing machine, and every group of film-making dyeing unit moves liquid syringe by 6 samples of a row and moves liquid syringe seat, 6 cell-preservation liquid bottles of a row and preserve liquid bottle seat and film-making plate that a row can adorn the may command upset in 6 sedimentation film-making storehouses is formed.
On the worktable of the utility model liquid basal cell film-making overflow dyeing machine kit is installed; Having 5 reagent troughs, kits to have port lid, kit not to have port lid, kit left side on the kit is that 6 reagent of a row move liquid syringe and move the liquid syringe seat, and the volume of each reagent trough is 250 to 300 milliliters.
The cell-preservation liquid bottle of installing on the worktable of the utility model liquid basal cell film-making overflow dyeing machine has bottle cap; The top of bottle is cylindric and is coniform with the bottom; Be built-in with and filter inner core and sample collection brush, the bottom of filtering inner core is welded with the nylon leaching net of mesh size between 50 to 200 orders.
Beneficial effect: the utility model liquid basal cell film-making overflow dyeing machine neither needs loaded down with trivial details LCT specific gravity liquid gradient centrifugation step and micro-tubular drainage system, does not also need the negative pressure of vacuum extraction of TCT and the process of miillpore filter malleation transitional cell.Utilization moves liquid syringe suction, mixing, cell dispersion sample and its accurate quantification imbibition and the fluid injection function realizes fluid sample and agent transfer operation, does not need the additional configuration oscillator.Cell in the sedimentation film-making storehouse relies on its gravity natural subsidence to having on the adhering microslide, carries out robotization dyeing then.Have simple to operately, cost is lower and make the characteristics of the quick high-quality of liquid based cytology smear.
Description of drawings
Fig. 1 is the contour structures synoptic diagram of liquid basal cell film-making overflow dyeing machine.
Fig. 2 is the planar structure synoptic diagram of liquid basal cell film-making overflow dyeing machine worktable.
Fig. 3 is the structural representation of facing of liquid basal cell film-making overflow dyeing machine.
Fig. 4 is the side-looking structural representation of liquid basal cell film-making overflow dyeing machine.
Fig. 5 is the structural representation of liquid basal cell film-making overflow dyeing machine kit.
Fig. 6 is the structural representation of cell-preservation liquid bottle.
Fig. 7 is the structural representation in film-making plate and sedimentation film-making storehouse.
Embodiment
For concrete structure and the content of understanding the utility model more carefully, for example the utility model is described further below in conjunction with accompanying drawing.
The structural representation of the liquid basal cell film-making overflow dyeing machine of Fig. 1, Fig. 2, Fig. 3 and different visual angles shown in Figure 4, its feature structure comprise machine base 1, worktable 2,6 samples of a row move liquid syringe and move liquid syringe seat 3,6 cell-preservation liquid bottles of a row and preserve liquid bottle seat 4, a row can adorn film-making plate 5, the reagent of the may command upset in 6 sedimentation film-making storehouses move liquid syringe and move liquid syringe seat 6, kit 7, micro computer kinetic control system 8, fuselage 9, kit have port lid 10, forearm 11, horizontal slide rail 12, movement arm 13, horizontal motor movement device 14, horizontal motor 15, up and down motor telecontrol equipment 16, move liquid syringe operation paw 17, top board 18 and side arm 19.
Fig. 5 is the structural representation of liquid basal cell film-making overflow dyeing machine kit.Having 5 reagent trough 20-24, kits to have port lid 10, kit not to have port lid 25, kit left side on the kit 7 is that 6 reagent of a row move liquid syringe and move liquid syringe seat 6, and the volume of each reagent trough is 250 to 300 milliliters.
Fig. 6 is the structural representation of cell-preservation liquid bottle.Comprise and filter inner core 26, the bottom of filtering inner core is welded with the nylon leaching net 27 of mesh size between 50 to 200 orders.To filter inner core 26 and insert in the cell-preservation liquid bottle 4, the characteristic of preserving the liquid bottle is that top is cylindric and bottom and is coniform 28, fills 10 to 15 ml cells and preserves liquid, puts into bottle to the brush that collects sample 30, builds bottle cap 29.
Fig. 7 is the structural representation in settlement plate and sedimentation film-making storehouse.A row can install 6 microslides and sedimentation film-making storehouse on the film-making plate 5.The microslide 34 of numbering is placed on the film-making plate 6 exactly, and each microslide 34 is put into corresponding microslide groove 35, the symmetrical ear in sedimentation film-making storehouse 32 is picked up the ears two semi-rings on 33 screw-in film-making plate slide groove both sides in 36, and fix.
Further specify below in conjunction with the structure accompanying drawing of liquid basal cell film-making overflow dyeing machine the course of work and principle the utility model.
Like Fig. 6 and shown in Figure 7, there is the brush 30 of official's neck brush of cell sample to be retained in the cell-preservation liquid bottle 4 collection, build bottle cap, be sent to the laboratory.When the laboratory operation personnel receive preservation liquid bottle; Jolt preservation liquid bottle for several times with hand; After 5 minutes, screw the cap off a bottle 29 with the centrifugal preservation liquid of 2000 rev/mins speed bottle, will filter inner core 26 and abandon from preserving to take out in the liquid bottle 4 in the lump with brush 30; Carefully pour out upper liquid lightly, observing bottle point bottom has cell precipitation 31.Then, with preserving in the preservation liquid bottle seat that liquid bottle 4 is placed on the worktable 2 in order, and with film-making plate 5 on the sample number position of microslide corresponding.
Start energized, computer are according to the programming automation complete operation of writing input, and the operation of machine single can be accomplished the film-making and the dyeing of 24 routine samples at most automatically.As shown in Figure 1; Movement arm 13 drives move liquid syringe operation paw 17 and are moved horizontally to the top position that reagent moves liquid syringe 6; Grasp reagent and move liquid syringe 6; From the buffering liquid groove 20 of kit 7, extract damping fluid, horizontal left is to the top position that every row preserves liquid bottle 4, and syringe injects 1 milliliter of damping fluid in every row's preservation liquid bottle.Then, movement arm 13 drives and moves liquid syringe operation paw 17 levels and move to right and reagent is moved liquid syringe be put back into original position 6.Movement arm 13 drives once more and moves the top position that the moving paraphase of liquid syringe operation paw 17 horizontal left should every stock layout originally moves liquid syringe 3; Grasp sample and move liquid syringe 3; Be inserted in the cell-preservation liquid bottle 4, and liquid is for several times drawn and is shifted about 0.3 to 0.6 milliliters of liquid to the film-making plate 5 in the corresponding sedimentation film-making storehouse in the suction bottle; Wait for the cell natural subsidence after 10 to 20 minutes, film-making plate upset is automatically outwelled Reversion Level original position behind the liquid in the storehouse.Movement arm 13 drive move liquid syringe operation paw 17 again sub-level be shifted to the right to the top position that reagent moves liquid syringe 6, grasp reagent downwards and move liquid syringe 6.Then, repeat from each reagent trough 20-24 of kit 7, to extract respectively the dye liquor and the reagent of aequum, join again in the sedimentation film-making storehouse on every row's film-making plate, finally accomplish the dyeing of cell smear.Behind reagent or water flushing sedimentation film-making storehouse inner room, take off sedimentation film-making storehouse and slide from the film-making plate, carry out mounting and microscopy again.
Claims (5)
1. one kind is used injection to move the liquid basal cell film-making overflow dyeing machine of liquid principle, its feature structure comprise that machine base (1), worktable (2), sample move liquid syringe and the film-making plate (5), the reagent that move liquid syringe seat (3), cell-preservation liquid bottle and preserve liquid bottle seat (4), may command upset move liquid syringe and move liquid syringe seat (6), kit (7), micro computer kinetic control system (8), fuselage (9), kit have port lid (10), forearm (11), horizontal slide rail (12), movement arm (13), horizontal motor movement device (14), horizontal motor (15), up and down motor telecontrol equipment (16), move liquid syringe operation paw (17), top board (18) and side arm (19).
2. as claimed in claim 1ly a kind ofly use injection to move the liquid basal cell film-making overflow dyeing machine of liquid principle; The fuselage (9) and the forearm (11) that it is characterized in that it support one group of horizontal slide rail (12); Movement arm (13) is across between two tracks of horizontal slide rail, and horizontal motor movement device (14) and motor telecontrol equipment (16) and move liquid syringe operation paw (17) up and down are housed on the movement arm.
3. as claimed in claim 1ly a kind ofly use injection to move the liquid basal cell film-making overflow dyeing machine of liquid principle; It is characterized in that being equipped with on its worktable (2) four groups of film-makings dyeing unit, every group of film-making dyeing unit moves liquid syringe by 6 samples of a row and moves liquid syringe seat, 6 cell-preservation liquid bottles of a row and preserve liquid bottle seat and film-making plate that a row can adorn the may command upset in 6 sedimentation film-making storehouses is formed.
4. as claimed in claim 1ly a kind ofly use injection to move the liquid basal cell film-making overflow dyeing machine of liquid principle; It is characterized in that on its worktable (2) kit (7) being installed; Having 5 reagent troughs (20-24), kit to have port lid (10), kit not to have port lid (25), kit left side on the kit is that 6 reagent of a row move liquid syringe and move liquid syringe seat (6), and the volume of each reagent trough is 250 to 300 milliliters.
5. as claimed in claim 1ly a kind ofly use injection to move the liquid basal cell film-making overflow dyeing machine of liquid principle; The last cell-preservation liquid bottle of installing (4) of worktable (2) that it is characterized in that it has bottle cap (29); The top of bottle is cylindric and is coniform (28) with the bottom; Be built-in with and filter inner core (26) and sample collection brush (30), the bottom of filtering inner core (26) is welded with the nylon leaching net (27) of mesh size between 50 to 200 orders.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201120430044XU CN202362187U (en) | 2011-11-03 | 2011-11-03 | Liquid-based cell slide preparation and dyeing machine based on injection pipetting principle |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201120430044XU CN202362187U (en) | 2011-11-03 | 2011-11-03 | Liquid-based cell slide preparation and dyeing machine based on injection pipetting principle |
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| CN202362187U true CN202362187U (en) | 2012-08-01 |
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| CN201120430044XU Expired - Fee Related CN202362187U (en) | 2011-11-03 | 2011-11-03 | Liquid-based cell slide preparation and dyeing machine based on injection pipetting principle |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105132274A (en) * | 2015-09-22 | 2015-12-09 | 陕西延长石油(集团)有限责任公司研究院 | Full-automatic bacteria disappearing experimental instrument |
| CN105866123A (en) * | 2016-04-29 | 2016-08-17 | 李少军 | Biological cell sap test box provided with filter membrane |
| CN109297796A (en) * | 2018-12-19 | 2019-02-01 | 孝感市森茂激光数控设备有限公司 | A kind of sampling of automatic sedimentation pelleter and dye liquor injection device and method |
| CN109520805A (en) * | 2018-11-27 | 2019-03-26 | 武汉医尔特科技有限公司 | A kind of full-automatic liquid-based cell sample manufacturing dyeing all-in-one machine |
| CN110940568A (en) * | 2019-12-17 | 2020-03-31 | 武汉友芝友医疗科技股份有限公司 | Integrated system for cell enrichment, separation, dyeing and flaking |
| CN111337321A (en) * | 2014-04-28 | 2020-06-26 | 深圳迈瑞生物医疗电子股份有限公司 | Smear preparation device and smear preparation method |
| CN111751193A (en) * | 2020-07-02 | 2020-10-09 | 郑州中普医疗器械有限公司 | Glass slide upright semi-immersion fixed type constant-temperature dyeing bin unit and dyeing machine |
| CN111855333A (en) * | 2019-04-24 | 2020-10-30 | 青岛北大新世纪言鼎生物医学科技有限公司 | Integrated reaction device and method for absorbing, printing, enriching and absorbing, printing and dyeing nucleated cells in body fluid |
| CN112179738A (en) * | 2020-09-30 | 2021-01-05 | 易普森生物科技(深圳)有限公司 | Novel cell pelleter |
| CN112557150A (en) * | 2020-12-11 | 2021-03-26 | 兴宏业（武汉）科技有限公司 | Biological cell dyeing machine |
| CN113533005A (en) * | 2021-07-27 | 2021-10-22 | 湖北泰康医疗设备有限公司 | A fast tablet dyeing machine |
| CN118913846A (en) * | 2024-10-09 | 2024-11-08 | 南通优耐特实验器材有限公司 | Slide glass dyeing device for biological analysis |
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2011
- 2011-11-03 CN CN201120430044XU patent/CN202362187U/en not_active Expired - Fee Related
Cited By (16)
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| CN111337321A (en) * | 2014-04-28 | 2020-06-26 | 深圳迈瑞生物医疗电子股份有限公司 | Smear preparation device and smear preparation method |
| CN105132274A (en) * | 2015-09-22 | 2015-12-09 | 陕西延长石油(集团)有限责任公司研究院 | Full-automatic bacteria disappearing experimental instrument |
| CN105866123A (en) * | 2016-04-29 | 2016-08-17 | 李少军 | Biological cell sap test box provided with filter membrane |
| CN109520805A (en) * | 2018-11-27 | 2019-03-26 | 武汉医尔特科技有限公司 | A kind of full-automatic liquid-based cell sample manufacturing dyeing all-in-one machine |
| CN109297796A (en) * | 2018-12-19 | 2019-02-01 | 孝感市森茂激光数控设备有限公司 | A kind of sampling of automatic sedimentation pelleter and dye liquor injection device and method |
| CN111855333A (en) * | 2019-04-24 | 2020-10-30 | 青岛北大新世纪言鼎生物医学科技有限公司 | Integrated reaction device and method for absorbing, printing, enriching and absorbing, printing and dyeing nucleated cells in body fluid |
| CN111855333B (en) * | 2019-04-24 | 2023-10-03 | 青岛言鼎生物医疗科技有限公司 | Device and method for integrating enrichment and dye-printing absorption and dyeing of nucleated cells in body fluid |
| CN110940568A (en) * | 2019-12-17 | 2020-03-31 | 武汉友芝友医疗科技股份有限公司 | Integrated system for cell enrichment, separation, dyeing and flaking |
| CN110940568B (en) * | 2019-12-17 | 2022-02-18 | 武汉友芝友医疗科技股份有限公司 | Integrated system for cell enrichment, separation, dyeing and flaking |
| CN111751193B (en) * | 2020-07-02 | 2021-09-28 | 郑州中普医疗器械有限公司 | Glass slide upright semi-immersion fixed type constant-temperature dyeing bin unit and dyeing machine |
| CN111751193A (en) * | 2020-07-02 | 2020-10-09 | 郑州中普医疗器械有限公司 | Glass slide upright semi-immersion fixed type constant-temperature dyeing bin unit and dyeing machine |
| CN112179738A (en) * | 2020-09-30 | 2021-01-05 | 易普森生物科技(深圳)有限公司 | Novel cell pelleter |
| CN112179738B (en) * | 2020-09-30 | 2021-07-13 | 易普森生物科技(深圳)有限公司 | Novel cell pelleter |
| CN112557150A (en) * | 2020-12-11 | 2021-03-26 | 兴宏业（武汉）科技有限公司 | Biological cell dyeing machine |
| CN113533005A (en) * | 2021-07-27 | 2021-10-22 | 湖北泰康医疗设备有限公司 | A fast tablet dyeing machine |
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120801 Termination date: 20181103 |
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| CF01 | Termination of patent right due to non-payment of annual fee |