CN107746799B - Handheld dry cell resuscitator - Google Patents
Handheld dry cell resuscitator Download PDFInfo
- Publication number
- CN107746799B CN107746799B CN201711165622.XA CN201711165622A CN107746799B CN 107746799 B CN107746799 B CN 107746799B CN 201711165622 A CN201711165622 A CN 201711165622A CN 107746799 B CN107746799 B CN 107746799B
- Authority
- CN
- China
- Prior art keywords
- handle
- heating body
- heating member
- dry cell
- heating
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 238000010438 heat treatment Methods 0.000 claims abstract description 72
- 238000007710 freezing Methods 0.000 claims abstract description 36
- 230000008014 freezing Effects 0.000 claims abstract description 36
- 239000004020 conductor Substances 0.000 claims abstract description 11
- 239000002470 thermal conductor Substances 0.000 claims description 6
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 5
- 239000000741 silica gel Substances 0.000 claims description 5
- 229910002027 silica gel Inorganic materials 0.000 claims description 5
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 3
- 239000000919 ceramic Substances 0.000 claims description 3
- 229910021389 graphene Inorganic materials 0.000 claims description 3
- 230000002093 peripheral effect Effects 0.000 claims description 3
- 230000000694 effects Effects 0.000 abstract description 8
- 238000005138 cryopreservation Methods 0.000 description 12
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- 239000002184 metal Substances 0.000 description 5
- 239000007788 liquid Substances 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 238000003339 best practice Methods 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
Abstract
The invention discloses a handheld dry cell resuscitator, which comprises a handle and a heating body, wherein the handle comprises a handle fixed part and a handle moving part, the heating body comprises a heating body supporting part and a heating body cover part, the handle fixed part is hinged with the handle moving part through a handle shaft, the handle moving part can be closed or separated on the handle fixed part around the handle shaft, the handle fixed part is forwards extended and provided with the heating body supporting part, the handle moving part is forwards extended and provided with the heating body cover part, the heating body supporting part and the heating body cover part are both provided with heat conductors, and the contact surfaces of the heating body supporting part and the heating body cover part are provided with freezing storage tube groove cavities. The handheld dry cell resuscitator provided by the invention has the advantages of simple structure, convenience in handheld operation, tight contact between the freezing storage tube and the heating body, no seam, good heating effect and good cell resuscitator effect of the freezing storage tube.
Description
Technical Field
The invention relates to the technical field of bioscience equipment, in particular to a handheld dry cell resuscitator.
Background
In the field of cell biology, an important way to preserve cell biological resources is to freeze in liquid nitrogen, namely, freeze cells in a liquid nitrogen tank below-170 ℃, and when the experiment is needed, take out the frozen cells for resuscitation. However, in the case of cell resuscitation, the best practice is to rapidly heat up the cell freezer tube just removed from the liquid nitrogen tank to 37 ℃ to reduce damage to the cells. In the resuscitation procedure, the cell freezer tube was held in the hand and heated in a 37 ℃ water bath.
In the field of cell biology, an important way to preserve cellular biological resources is low-temperature freezing, and when the experiment needs, the frozen cells are taken out for resuscitation. When the cells are recovered, the best operation is to quickly heat the cell freezing tube to 37 ℃ so as to reduce damage to the cells. In actual work, cell resuscitating is mostly artificial water bath resuscitating, the operation flow is not easy to be standardized, and pollution is easy to be caused. There is a dry resuscitator for cell cryopreservation tubes on the market, but it is only suitable for dry resuscitating a cell cryopreservation tube of 2ml capacity. Currently, there is a lack of dry cell resuscitators on the market that are specifically adapted to cell cryopreservation tubes of greater than 2ml volume.
Disclosure of Invention
The present invention is directed to a hand-held dry cell resuscitator that overcomes one or more of the problems of the prior art described above.
The invention relates to a handheld dry cell resuscitator, which comprises a handle and a heating body, wherein the handle comprises a handle fixed part (1) and a handle moving part (2), the heating body comprises a heating body supporting part (3) and a heating body cover part (4), the handle fixed part (1) is hinged with the handle moving part (2) through a handle shaft (5), the handle moving part (2) can be closed or separated on the handle fixed part (1) around the handle shaft (5), the handle fixed part (1) is forwards extended and provided with the heating body supporting part (3), the heating body moving part (2) is forwards extended and provided with the heating body cover part (4), the heating body supporting part (3) and the heating body cover part (4) are both provided with heat conductors (6), and the contact surfaces of the heating body supporting part (3) and the heating body cover part (4) are provided with freezing storage tube groove cavities (7).
The handheld dry cell resuscitator provided by the invention has the advantages of simple structure, convenience in handheld operation, tight contact between the freezing storage tube and the heating body, no seam, good heating effect and good cell resuscitator effect of the freezing storage tube.
In some embodiments, the appearance of the freezing tube groove cavity (7) is long rectangular, the cross section of the freezing tube groove cavity is semicircular, and the inner peripheral wall surface of the freezing tube groove cavity (7) is lined with a heat conducting silica gel layer (71). The cell freezing tube is convenient to use and easy to disinfect, can be used for accommodating 1 cell freezing tube with the volume of 5ml for resuscitation, can also accommodate 2 cell freezing tubes with the volume of 2ml for resuscitation, and avoids pollution risks caused by water bath cell resuscitation.
In some embodiments, at least one temperature sensor (72) is embedded in the bottom of the freezing tube groove cavity (7). The instant temperature of the cell freezing tube in the thawing tube groove cavity (7) can be timely obtained through the temperature sensor.
In some embodiments, a control button (11) and a display screen (12) are arranged on the handle fixing part (1), the control button (11) and the display screen (12) are connected with the temperature sensor (72), and a power line (13) is arranged at the tail part of the handle fixing part (1). The thermal conductor can be regulated by a control button (11), and the real-time temperature detected by the temperature sensor (72) can be displayed on the display screen (12).
In some embodiments, a return spring (8) is arranged between the extending part of the handle fixed part (1) and the extending part of the handle moving part (2), one end of the return spring (8) is fixedly connected with the handle fixed part (1), and the other end of the return spring is fixedly connected with the handle moving part (2). Through reset spring's setting, can make the operation between handle fixed part and the handle movable part more convenient, the resilience force is good.
In some embodiments, the thermal conductor (6) is a peltier, graphene electrothermal film or ceramic heating plate. The design can provide dry heating, does not bring pollution risk to cell resuscitation, and has good heat conduction effect.
In some embodiments, the handle moving part (2) and the heating body cover part (4) are fixedly connected and naturally transited, and the heating body cover part (4) can be tightly closed with the heating body supporting part (3), so that the sealing tightness is good.
Drawings
FIG. 1 is a schematic diagram of a hand-held dry cell resuscitation apparatus, according to an embodiment of the present invention;
FIG. 2 is a schematic cross-sectional view of a thermal conductor of a handheld dry cytoresuscitation instrument of an embodiment of the present invention, separated;
FIG. 3 is a schematic cross-sectional view of a combination of thermal conductors of a handheld dry cytoresuscitation device of an embodiment of the present invention;
fig. 4 is a schematic top view of a cryopreservation vessel cavity of a handheld dry cell resuscitator according to an embodiment of the invention.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings.
As shown in fig. 1, a hand-held dry cell resuscitator comprises a handle and a heating body, wherein the handle comprises a handle fixed part (1) and a handle movable part (2), the heating body comprises a heating body supporting part (3) and a heating body cover part (4), and the heating body cover part (4) can be covered on the heating body supporting part (3); the handle fixed part (1) is hinged with the handle moving part (2) through a handle shaft (5), and the handle moving part (2) can perform closing or opening actions on the handle fixed part (1) around the handle shaft (5); the handle fixed part (1) extends forward and is connected with the heating body support part (3), the handle movable part (2) extends forward and is provided with the heating body cover part (4), the heating body support part (3) and the heating body cover part (4) are both provided with the heat conductor (6), the heat conductor (6) is a Peltier, a graphene electrothermal film or a ceramic heating plate, the design can provide dry heating, pollution risks can not be brought to cell resuscitating, and the heat conduction effect is good.
As shown in fig. 2 to 4, the contact surfaces of the heating body supporting part (3) and the heating body cover part (4) are provided with the freezing tube groove cavity (7), the appearance of the freezing tube groove cavity (7) is long rectangular, the cross section of the freezing tube groove cavity is semicircular, after the heating body supporting part (3) and the heating body cover part (4) are closed, the freezing tube groove cavity (7) forms a cylindrical inner cavity, the surface of the inner peripheral wall of the freezing tube groove cavity (7) is lined with the heat conducting silica gel layer (71), the structure is simple, the use is convenient, the sterilization is easy, the freezing tube groove cavity (7) can be used for accommodating 1 cell freezing tube with the volume of 5ml for resuscitation, 2 cell freezing tubes with the volume of 2ml for resuscitation can be simultaneously accommodated, and the pollution risk caused by water bath cell resuscitation is avoided.
At least one temperature sensor (72) is embedded in the bottom of the freezing tube groove cavity (7), in the embodiment, two temperature sensors (72) are installed, the temperature in the freezing tube groove cavity (7) can be timely detected through a stable sensor (), the temperature sensors (72) in the heating body supporting part (3) and the temperature sensors (72) of the heating body cover part (4) are arranged in a vertically corresponding or mutually staggered mode, and the instant temperature of the cell freezing tube of the freezing tube groove cavity (7) can be better detected.
A control button (11) and a display screen (12) are arranged on the handle fixed part (1), the control button (11) and the display screen (12) are connected with a temperature sensor (72), and a power line (13) is arranged at the tail part of the handle fixed part (1). The thermal conductor can be regulated by a control button (11), and the real-time temperature detected by the temperature sensor (72) can be displayed on the display screen (12).
A reset spring (8) is arranged between the extending part of the handle fixed part (1) and the extending part of the handle movable part (2), one end of the reset spring (8) is fixedly connected with the handle fixed part (1), the other end is fixedly connected with the handle movable part (2), and the handle fixed part (1) and the handle movable part (2) are more convenient to operate and good in resilience through the arrangement of the reset spring (8). The handle moving part (2) is fixedly connected with the heating body cover part (4), the heating body cover part (4) can be tightly closed with the heating body supporting part (3) in a natural transition way, and the sealing tightness is good.
The handheld dry cell resuscitator provided by the invention has the advantages of simple structure, convenience in handheld operation, tight contact between the freezing storage tube and the heating body, no seam, good heating effect and good cell resuscitator effect of the freezing storage tube.
Example 1:
in this embodiment, when the cell cryopreservation tube is required to be resuscitated, the handheld dry cell resuscitator is powered on, a preheating program is selected, the metal heat conductor (6) of the supporting part and the metal heat conductor (6) of the cover part are preheated to a set temperature, the cell cryopreservation bag is placed in the cryopreservation tube groove cavity (7) of the metal heat conductor (6) of the supporting part, the handle fixing part (1) and the handle moving part (2) are held, the handle fixing part (1) and the handle moving part (2) are made to move in opposite directions, the heating body cover part (4) and the heating body supporting part (3) are driven to be closed, and the heat conducting silica gel layer (71) on the surface of the cryopreservation tube groove cavity (7) is self-adaptive to the shape of the cell cryopreservation tube, so that an air gap is completely absent between the heating body cover part (4) and the heating body supporting part (3), and the heat conduction is facilitated.
After the cell cryopreservation tube is placed in the resuscitation groove cavity, the handle part of the cell cryopreservation tube is immediately held by hands to shake with proper force and amplitude, so that melted liquid is promoted to be evenly mixed in time, the cell cryopreservation tube is heated evenly, and the cell resuscitation survival rate is improved.
Example 2:
in this embodiment, the structures of the supporting metal heat conductor (6) and the cover metal heat conductor (6) of the heating body part described in the foregoing embodiment 1 are replaced by flat-bottom shallow square plate-shaped heat conductors, and a layer of heat-conducting silica gel layer (71) is lined on the surface, so that the cell freezing bag can be used for resuscitation, and the use method is the same as that of embodiment 1.
The foregoing is merely a preferred form of the invention, and it should be noted that several similar variations and modifications could be made by those skilled in the art without departing from the inventive concept, which are also considered to be within the scope of the invention.
Claims (7)
1. The utility model provides a handheld dry-type cyto-resuscitation appearance, its characterized in that, includes handle and heating member, the handle includes handle fixed part (1) and handle movable part (2), the heating member includes heating member support portion (3) and heating member lid (4), handle fixed part (1) with it is articulated through handle axle (5) between handle movable part (2), handle movable part (2) can be in on handle fixed part (1) around handle axle (5) carries out closure or opens the action, handle fixed part (1) forward extension is equipped with heating member support portion (3), handle movable part (2) forward extension is equipped with heating member lid (4), heating member support portion (3) with all be equipped with heat conductor (6) on heating member lid (4), freezing storage tube chamber (7) have all been seted up to the contact surface of heating member support portion (3) with heating member lid (4).
2. The handheld dry cell resuscitator according to claim 1, wherein the freezing tube groove cavity (7) is long rectangular, the cross section of the freezing tube groove cavity is semicircular, and the surface of the inner peripheral wall of the freezing tube groove cavity (7) is lined with a heat conducting silica gel layer (71).
3. The hand-held dry cell resuscitator according to claim 2, wherein at least one temperature sensor (72) is embedded in the bottom of the freezing tube cavity (7).
4. A hand-held dry cell resuscitation apparatus according to claim 3, wherein said handle portion (1) is provided with a control button (11) and a display screen (12), said control button (11) and said display screen (12) are connected to said temperature sensor (72), and said handle portion (1) is provided with a power cord (13) at the tail thereof.
5. The hand-held dry cell resuscitation apparatus according to claim 1, wherein a return spring (8) is disposed between the extension portion of the handle fixing portion (1) and the extension portion of the handle moving portion (2), and one end of the return spring (8) is fixedly connected with the handle fixing portion (1), and the other end is fixedly connected with the handle moving portion (2).
6. A hand-held dry cytoresuscitation instrument according to claim 1, characterized in that said thermal conductor (6) is a peltier, graphene electrothermal film or ceramic heating plate.
7. The hand-held dry cell resuscitator according to claim 1, wherein the handle moving part (2) is fixedly connected with the heating body cover part (4), and the heating body cover part (4) can be tightly closed with the heating body supporting part (3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711165622.XA CN107746799B (en) | 2017-11-21 | 2017-11-21 | Handheld dry cell resuscitator |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711165622.XA CN107746799B (en) | 2017-11-21 | 2017-11-21 | Handheld dry cell resuscitator |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107746799A CN107746799A (en) | 2018-03-02 |
| CN107746799B true CN107746799B (en) | 2023-10-13 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711165622.XA Active CN107746799B (en) | 2017-11-21 | 2017-11-21 | Handheld dry cell resuscitator |
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| Country | Link |
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| CN (1) | CN107746799B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110305786A (en) * | 2019-06-20 | 2019-10-08 | 大连理工大学 | A kind of hand-held, can be from the dry type cell resuscitator of disinfection |
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| CN102586104A (en) * | 2011-12-23 | 2012-07-18 | 北京瑞建天行生物技术有限公司 | Multifunctional container for freezing cells and resurrecting frozen cells |
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| CN107746799A (en) | 2018-03-02 |
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Effective date of registration: 20230908 Address after: 210000 Room A249, 2nd Floor, C Creation Space, No. 7 Nanyou Road, Jiangning District, Nanjing City, Jiangsu Province (Jiangning Development Zone) Applicant after: New key (Nanjing) Biotechnology Co.,Ltd. Address before: Room 209, area B, 2 / F, building 2, No.58 Xiangke Road, pilot Free Trade Zone, Xuhui District, Shanghai Applicant before: THERMBEST BIOENGINEERING (SHANGHAI) CO.,LTD. |
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Effective date of registration: 20240205 Address after: Room 308, No. 42, Guangzhou Road, Zhongxing Street, Development Zone, Nantong City, Jiangsu Province, 226000 Patentee after: Mingchang Biopharmaceutical Nantong Co.,Ltd. Country or region after: China Address before: 210000 Room A249, 2nd Floor, C Creation Space, No. 7 Nanyou Road, Jiangning District, Nanjing City, Jiangsu Province (Jiangning Development Zone) Patentee before: New key (Nanjing) Biotechnology Co.,Ltd. Country or region before: China |