CN211339531U - Instrument device for anhydrous dry-type resuscitation of cells - Google Patents
Instrument device for anhydrous dry-type resuscitation of cells Download PDFInfo
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- CN211339531U CN211339531U CN201920977119.2U CN201920977119U CN211339531U CN 211339531 U CN211339531 U CN 211339531U CN 201920977119 U CN201920977119 U CN 201920977119U CN 211339531 U CN211339531 U CN 211339531U
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Abstract
The utility model discloses an instrument device for anhydrous dry-type recovery of cell, including recovery cabin, base and collet formula mixing device, recovery cabin is located the base top, and recovery cabin includes the recovery cabin body and swing joint in the recovery cabin body's recovery cabin lid, and recovery cabin lid can be covered and fits the recovery cabin body, forms inclosed recovery space, and recovery cabin internal is equipped with the compound temperature tray of duplex, is equipped with the compound temperature lid of duplex in the recovery cabin lid, and in the base was located according to the power supply of kneading even device to the collet formula, through power support and the compound temperature tray swing joint of duplex. The utility model is suitable for an anhydrous dry-type of cell cryopreserving bag or cell cryopreserving pipe is resumeed and is used.
Description
Technical Field
The utility model relates to a cell recovery field, concretely relates to an instrument device that is used for anhydrous dry-type of cell to come back to life or consciousness.
Background
With the development of life technologies, cell therapy technologies are changing day by day, and mainly include stem cell therapies and immune cell therapies typified by CAR-T cells. The preparation of cell preparations involves cryopreservation and thawing of the cells. When the cells are thawed for recovery, the best operation is that the cell freezing bag or the cell freezing tube which is just taken out from the liquid nitrogen tank is quickly heated to 37 ℃, so that the cells can quickly pass through-5 ℃ to 0 ℃ which is most easily damaged by the cells, and the cells are prevented from being damaged by the recrystallization of the ice crystals, and the cells are prevented from being killed.
Cell cryopreservation containers are usually a cell cryopreservation tube and a cell cryopreservation bag. The freezing capacity of the cell freezing tube is less than 5ml, and more than 5ml of cell freezing bags are selected for cell freezing requirements. The cell cryopreservation bag is more suitable for the commercial application of stem cells and immune cells, such as CAR-T cell therapy. At present, the cell cryopreservation bag can be recovered by adopting a water bath recovery mode or a dry recovery mode, wherein the water bath recovery mode needs manual operation in the whole process, can not carry out standardized operation, easily pollutes cells and is gradually eliminated for use, the dry recovery mode is limited in products at present, the recovery mode is single, the recovery efficiency is not satisfactory, and the improvement and development requirements are great.
In any heating device, the heat source transfers heat energy to the heated object in three forms of convection, radiation and conduction. Heat conduction refers to the phenomenon of heat transfer that occurs when there is no relative displacement between parts of an object or when different objects are in direct contact, depending on the thermal motion of microscopic particles, such as molecules, atoms, and free electrons of a substance. Convection is a phenomenon that relies on the movement of a fluid to transfer heat from one place to another. Whether conductive or convective, heat must be transferred by direct contact of hot and cold objects or by means of conventional substances. The principle of thermal radiation is quite different, however, and it relies on the object surface emitting visible and invisible radiation to the outside to transfer heat. The radiation heating has high transfer speed and does not pass through any medium, thereby greatly reducing the loss in the heat energy transfer process and improving the heat energy utilization rate.
The far infrared heating technology mainly emphasizes radiation heating. When far infrared rays are irradiated to an object to be heated, a part of the rays are reflected and a part of the rays are transmitted. When the wavelength of the emitted far infrared ray is consistent with the absorption wavelength of the heated object, the heated object absorbs a large amount of far infrared ray, so that partial molecules and atoms in the object generate resonance to generate strong vibration and rotation, and the vibration and the rotation increase the temperature of the object, thereby achieving the heating purpose.
Far infrared is a light invisible to the naked eye, which is different from general infrared (near infrared) which has only a thermotherapy effect on the human body; it converts the ambient heat energy into far infrared radiation efficiently through specific infrared material; the far infrared rays with a wavelength of 3-10 μm can be absorbed by water molecules and organic molecules in the living body, so that energy can be transmitted into the living body to induce a series of physiological actions beneficial to human health. By using this mechanism, far infrared rays can be widely used in medical care, which is called "far infrared therapy".
The energy emission mode of the carbon crystal electric heating plate or the carbon fiber electric heating plate is mainly far infrared radiation, and infrared energy capable of heating substances can be radiated from the carbon crystal electric heating plate or the carbon fiber electric heating plate after the carbon crystal electric heating plate or the carbon fiber electric heating plate is electrified. The wavelength of the infrared radiation is 2.3 to 14 μm, and the far infrared spectrum of this wavelength is called "life light" and accounts for more than 80% of the total wavelength. It can be absorbed by water molecule to generate resonance friction heat effect, and can quickly raise the temp. of heated material. Especially has the health care effects of effectively activating human tissue cells, promoting blood circulation, accelerating new metabolism, enhancing immunity and the like.
At present, no anhydrous dry resuscitation application for simultaneously performing cell cryopreservation bags or cell cryopreservation tubes by utilizing warm plate conduction heating and far infrared radiation heating is available. The utility model discloses consequently come.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing an instrument device for anhydrous dry-type resuscitation of cell solves one or more among the above-mentioned prior art problem.
The instrument device for the anhydrous dry-type cell resuscitation comprises a resuscitation cabin, a base and a bottom-mounted mixing device, wherein the resuscitation cabin is positioned above the base;
the recovery cabin comprises a recovery cabin body and a recovery cabin cover movably connected to the recovery cabin body, the recovery cabin cover can cover the recovery cabin body to form a closed recovery space, a double-work rewarming tray is arranged in the recovery cabin body, and a double-work rewarming heat cover is arranged in the recovery cabin cover;
the bottom-supported blending device is arranged in the base and is connected with the double-function rewarming tray in the resuscitation cabin body.
The instrument device for the anhydrous dry recovery of the cells, provided by the utility model, can be used for the anhydrous dry recovery of the cell freezing bag and the anhydrous dry recovery of the high-flux cell freezing tube; meanwhile, two rewarming modes of contact conduction heating and far infrared radiation heating are integrated, so that the rewarming efficiency of the cell cryopreservation bag or the cell cryopreservation tube is higher.
In some embodiments, the double-work rewarming tray is detachable and replaceable, a buffer device and a pressure sensor are arranged between the double-work rewarming cover and the resuscitation cover, the double-work rewarming cover and the resuscitation cover are movably connected through the buffer device and the pressure sensor, the double-work rewarming cover is detachable and replaceable, and an exhaust fan is further arranged in the resuscitation cover.
In some embodiments, air is sucked from the ventilation openings of the bottom, the side walls and the rear wall of the base, ascends along the gap inside the resuscitation chamber cavity and is exhausted by the exhaust fan, and the base is provided with a numerical control device, a communication device and a touch screen.
In some embodiments, the dual-function rewarming tray comprises a tray frame and a first heating body, wherein the tray frame is made of metal, the first heating body is arranged in the tray frame, the first heating body is an electric heating plate which has two heating functions of conduction heating and far infrared radiation heating, the first heating body is fixedly connected with the tray frame, the tray frame is provided with first temperature control temperature sensors, the number of the first temperature control temperature sensors is 1-6, the first heating body is provided with through holes, first rewarming monitoring temperature sensors are arranged in the through holes, and the number of the first rewarming monitoring temperature sensors is 1-6;
the working temperature range of the double-function rewarming tray is 10-70 ℃, and the placing position guide line of the cell cryopreservation bag or the cell cryopreservation tube is drawn on the double-function rewarming tray.
In some embodiments, the temperature control temperature sensor and the rewarming monitoring temperature sensor include a contact temperature sensor and a non-contact temperature sensor, the contact temperature sensor includes a thermistor sensor, a thermocouple string sensor, a digital sensor, a platinum sensor, and the like, and the non-contact temperature sensor includes a thermopile array non-contact temperature sensor, an infrared temperature probe temperature sensor, an infrared thermal imager temperature sensor, and the like.
In some embodiments, the double-work rewarming heat cover includes a heat cover frame and a second heating member, the heat cover frame is made of metal, the second heating member is located in the heat cover frame, the second heating member is an electric heating plate having two heating functions of conduction heating and far infrared radiation heating, the second heating member is fixedly connected with the heat cover frame, the heat cover frame is provided with 1-6 second temperature control temperature sensors, the second heating member is provided with a through hole, a second rewarming monitoring temperature sensor is arranged in the through hole, the number of the second rewarming monitoring temperature sensors is 1-6, the working temperature range of the double-work rewarming heat cover is 10-70 ℃, and a positioning finger lead of a cell cryopreservation bag or a cell cryopreservation tube is drawn on the double-work rewarming heat cover.
In some embodiments, the damping device is a hydraulic damper, a spring damper, or a damping damper, and the damping damper includes an air-based damping damper, a gear-based damping damper, a rack-based damping damper, and a cylindrical damping damper.
In some embodiments, the pressure sensor is a piezoresistive pressure sensor, a ceramic pressure sensor, a diffused silicon pressure sensor, a sapphire pressure sensor, a piezoelectric pressure sensor.
In some embodiments, collet formula mixing device includes power supply and power support, the power supply pass through bolt fixed connection in the bottom in the base, the power supply includes servo motor and motor mount, servo motor's motor shaft end is equipped with the eccentric wheel, servo motor's figure is 2 or 4, the figure of power support is 4, the figure of eccentric wheel is 4, 4 the size specification of eccentric wheel is the same, can adjust 4 respectively during the installation the initial eccentricity of the vertical direction of eccentric wheel, the power support will the eccentric wheel with double-power rewarming tray passes through ball socket structure swing joint, servo motor's operation receives numerical control device's regulation and control, collet formula mixing device can drive double-power rewarming tray realizes that the collet formula is according to rubbing the oscillation mixing motion.
In some embodiments, the bottom-mounted blending device can be an electric fast hydraulic ejector rod, an electric hydraulic push rod, an electric push rod and the like, the number of the electric fast hydraulic ejector rods, the electric hydraulic push rods, the electric push rods and the like is two, the electric fast hydraulic ejector rods, the electric hydraulic push rods, the electric push rods and the like are respectively and movably connected with the double-power rewarming tray, and the operation of the electric fast hydraulic ejector rods, the electric hydraulic push rods, the electric push rods and the like is regulated and controlled by the numerical control device.
In some embodiments, the communication device can perform data exchange, data storage and data query with the internet or a local area network through wired or wireless networking.
A method of operating an instrument device for anhydrous dry resuscitation of cells, comprising the steps of:
(1) starting an instrument, setting the temperature required by recovery, selecting a recovery working mode, starting a preheating function, preheating a double-work rewarming tray and a double-work rewarming hot cover, starting the temperature rise of the double-work rewarming tray and the double-work rewarming hot cover, emitting far infrared rays, after the preheating is finished, placing a cell cryopreservation bag or a cell cryopreservation pipe on the double-work rewarming tray, covering and fixing a recovery cabin cover, clamping the cell cryopreservation bag or the cell cryopreservation pipe by the double-work rewarming tray and the double-work rewarming hot cover under the action of a buffer device between the double-work rewarming hot cover and the recovery cabin cover, starting cell recovery, simultaneously transferring heat to the cell cryopreservation bag or the cell cryopreservation pipe by two heat conduction modes of conduction and radiation by the double-work rewarming tray and the double-work rewarming hot cover, starting a bottom-support type blending device to work, and starting working angles of 4 eccentric wheels are different under the regulation and control of a numerical control device, the operation speeds are different, the double-work rewarming tray, the cell cryopreservation bag or the cell cryopreservation tube and the double-work rewarming hot cover are driven to perform bottom-supported kneading oscillation blending movement together, the force applied by the blending movement can be detected by a pressure sensor between the double-work rewarming hot cover and the resuscitation cabin cover and is transmitted to a numerical control device, the operation of a power source is intelligently fed back and adjusted, and the cell cryopreservation bag or an instrument is prevented from being damaged due to excessive force;
(2) the temperature of the double-power rewarming tray and the double-power rewarming hot cover is monitored at any time through the temperature control temperature sensor, detection data are transmitted to the numerical control device, the numerical control device controls the heating process of the double-power rewarming tray and the double-power rewarming hot cover, and the temperature is intelligently maintained at a set temperature;
(3) the rewarming monitoring temperature sensor detects whether the current temperature of the cell cryopreservation bag or the cell cryopreservation tube is matched with the set rewarming target temperature, the matching stability determination time is not more than 5 seconds, if the current temperature is lower than the rewarming target temperature, the heating rewarming state is started and maintained, and if the current temperature is higher than the rewarming target temperature, the heating is immediately stopped;
(4) when the cell freezing and storing device is in a heating mode, the double-power heating tray and the double-power temperature-restoring hot cover are in a heating state, the temperature of the tray body and the temperature of the hot cover are increased, far infrared rays are emitted, and heat is transferred to the cell freezing and storing bag or the cell freezing and storing pipe through two heat conduction modes of conduction and radiation to restore the temperature;
(5) when the rewarming monitoring temperature sensor detects that the temperature of the cell cryopreservation bag exceeds 0-8 ℃, the heating mode is closed after the cell cryopreservation bag or ice blocks in the cell cryopreservation pipe are completely melted, the double-power rewarming tray and the double-power rewarming heating cover stop working, the exhaust fan is started at the same time, air is sucked from the ventilation openings of the bottom, the side wall and the rear wall of the base, rises along the gap in the resuscitation cavity, is finally exhausted from the exhaust fan, takes away the heat of the double-power rewarming tray and the double-power rewarming heating cover, and the bottom-supported mixing device continuously performs bottom-supported kneading oscillation mixing movement;
(6) the cell thawing work of the cell freezing bag or the cell freezing tube is completed, and the operator is reminded to take away the thawed cell freezing bag or the cell freezing tube in time through acousto-optic signals such as indicator light flickering and loudspeaker sounding.
The utility model has the advantages and beneficial effects:
1. the utility model can be used for anhydrous dry resuscitation of the cell freezing bag and anhydrous dry resuscitation of the high-flux cell freezing tube;
2. the utility model uses an electric heating plate component which has two functions of flat heating and far infrared radiation heating, and integrates two rewarming modes of contact conduction heating and far infrared radiation heating, so that the rewarming efficiency of the cell freezing bag or the cell freezing tube is higher;
3. the double-work rewarming tray is arranged in the resuscitation cabin and can perform bottom-supported oscillation blending movement, the built-in blending working mode is compared with the external blending working mode, a sealing working cabin is not required to be arranged on the double-work rewarming tray, the whole weight of the double-work rewarming tray is light, the structure is simpler, and the technical realization is easy;
4. the number of the servo motors of the bottom-supported type mixing device of the four-point supported type double-work rewarming tray is 4, 4 eccentric wheels and the double-work rewarming tray are movably connected through 4 supporting points by utilizing 4 connecting rods, the operation of the servo motors is regulated and controlled by a numerical control device, and the four-point supported type double-work rewarming tray can realize complex and diversified bottom-supported type oscillating mixing motion by changing the rotating speed of the servo motors and the initial operating angle of the eccentric wheels, and the mixing effect of the four-point supported type double-work rewarming tray is obviously stronger than that of a two-point supported type seesaw type mixing effect of only 1 servo motor;
5. the bottom-support type blending device is matched with the buffer device in the resuscitation cabin cover, so that the cell freezing bag or the cell freezing pipe can be clamped by the double-function rewarming tray and the double-function rewarming hot cover, heat conduction is facilitated, the bottom-support type blending device can extrude the cell freezing bag, flowing and mixing of liquid in the cell freezing bag are facilitated, the liquid with high relative temperature and heated at the periphery can be timely blended with the liquid with low relative temperature and close to ice blocks in the cell freezing bag, cells are protected from being inactivated due to high temperature, heat can be brought to the periphery of the ice blocks rapidly and uniformly, and rapid thawing and thawing are achieved;
6. the recovery cabin cover and the double-work composite heating cover are provided with a buffer device and a pressure sensor, the force applied by the power source of the bottom-mounted blending device can be detected by the pressure sensor between the double-work composite heating cover and the recovery cabin cover and is transmitted to the numerical control device, the operation of the power source is intelligently fed back and adjusted, and the damage of a cell cryopreservation bag or an instrument caused by overlarge applied force is prevented;
7. an exhaust fan is further arranged in the resuscitation cabin cover, air is sucked in from the ventilation openings of the bottom, the side wall and the rear wall of the base, rises along gaps in the resuscitation cabin and is finally exhausted from the exhaust fan, heat of the double-power rewarming tray and the double-power rewarming cover is taken away, and influence of residual heat of the double-power rewarming tray and the double-power rewarming cover on the cell cryopreservation bag or the cell cryopreservation tube is favorably reduced after rewarming is finished.
Drawings
FIG. 1 is a schematic diagram of an instrument for dry cell resuscitation according to an embodiment of the present invention;
fig. 2 is an isometric view of a double-power rewarming tray, a double-power rewarming hot lid, a buffer device and a bottom-supported blending device of an instrument device for dry-type resuscitation of cells according to an embodiment of the present invention;
FIG. 3 is a top view of an instrument device for anhydrous dry resuscitation of cells, in accordance with an embodiment of the present invention;
fig. 4 is a right side view of a dual function rewarming tray, a dual function rewarming lid and a buffer device of an instrument device for anhydrous dry resuscitation of cells according to an embodiment of the present invention;
fig. 5 is a bottom view of the dual-function rewarming tray, the dual-function rewarming thermal cover, the buffering device and the bottom-supported mixing device of the apparatus for dry cell resuscitation according to an embodiment of the present invention.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings.
Example 1:
as shown in fig. 1, an instrument device for anhydrous dry cell resuscitation comprises a resuscitation chamber 1, a base 2 and a bottom-mounted blending device 3, wherein the resuscitation chamber 1 is installed above the base 2, the resuscitation chamber 1 comprises a resuscitation chamber body 11 and a resuscitation chamber cover 12 movably connected to the resuscitation chamber body 11, the resuscitation chamber cover 12 can cover the resuscitation chamber body 11 to form a closed resuscitation space, a double-work rewarming tray 111 is arranged in the resuscitation chamber body 11, a double-work rewarming thermal cover 121 is arranged in the resuscitation chamber cover 12, the double-work rewarming tray 111 and the double-work rewarming thermal cover 121 can be covered, and a cell cryopreservation bag or a cell cryopreservation tube can be placed between the double-work rewarming tray 111 and the double-work rewarming thermal cover 121 for resuscitation.
The bottom-supported mixing device 3 is a bottom-supported kneading and mixing device, the bottom-supported kneading and mixing device is installed in the base 2 and is connected with the double-work rewarming tray 111 in the resuscitation cabin body 11, the resuscitation cabin 1 is fixed in the embodiment, and the bottom-supported kneading and mixing device drives the double-work rewarming tray 111 to perform bottom-supported kneading and oscillating mixing movement.
The instrument device for the anhydrous dry recovery of the cells, provided by the utility model, can be used for the anhydrous dry recovery of the cell freezing bag and the anhydrous dry recovery of the high-flux cell freezing tube; meanwhile, two rewarming modes of contact conduction heating and far infrared radiation heating are integrated, so that the rewarming efficiency of the cell cryopreservation bag or the cell cryopreservation tube is higher.
In the above, as shown in fig. 2 to 4, the double-work rewarming tray 111 can be detached and replaced, a buffer device 122 and a pressure sensor 124 are installed between the double-work rewarming thermal cover 121 and the resuscitation flap 12, the double-work rewarming thermal cover 121 and the resuscitation flap 12 are movably connected through the buffer device 122 and the pressure sensor 124, the pressure sensor 124 is installed in the middle of or beside the buffer device 122, the buffer device 122 is a spring in this embodiment, one end of the buffer device is fixedly installed at four corners of the double-work rewarming thermal cover 121 through bolts, the other end of the buffer device is fixedly installed on the resuscitation flap 12 through bolts, the double-work rewarming thermal cover 121 can also be detached and replaced, and an exhaust fan 123 is further arranged. The double-work rewarming tray 111 and the double-work rewarming heat cover 121 simultaneously integrate two rewarming modes of contact conduction heating and far infrared radiation heating, and the rewarming efficiency is higher.
Air is sucked from the ventilation openings of the bottom, the side wall and the rear wall of the base 2, rises along the gap inside the resuscitation chamber 1 cavity and is exhausted by the exhaust fan 123, and the base 2 is provided with the numerical control device 21, the communication device 22 and the touch screen 23.
The double-power rewarming tray 111 comprises a tray frame 1111 and a first heating body 1112, the tray frame 1111 is made of metal, the first heating body 1112 is installed in the tray frame 1111, the first heating body 1112 is an electric heating plate with two heating functions of conduction heating and far infrared radiation heating, the electric heating plate comprises a carbon crystal electric heating plate, a carbon fiber electric heating plate and the like, the first heating body 1112 is fixedly connected with the tray frame 1111 through a bolt, a first temperature control temperature sensor is arranged in the tray frame 1111 and can monitor the temperature of the first heating body 1112 in real time, the number of the first temperature control temperature sensors is 1-6, a through hole is formed in the first heating body 1112, a first rewarming monitoring temperature sensor 1114 is arranged in the through hole and can monitor the temperature of a cell freezing bag in real time, the number of the first rewarming monitoring temperature sensors 1114 is 1-6, and the number is 2 in the embodiment.
The working temperature range of the double-function rewarming tray 111 is 10-70 ℃, the working temperature range can be set through the touch screen 23, the finger guide lines for placing the cell freezing bags or the cell freezing tubes are drawn on the double-function rewarming tray 111, and the double-function rewarming tray 111 can be a flat tray and is suitable for dry recovery of the cell freezing bags.
The double-function rewarming tray 111 can also be provided with a plurality of cryopreservation pipe groove cavities, the cryopreservation pipe groove cavities are long rectangular, the cross sections of the cryopreservation pipe groove cavities are semicircular, and the double-function rewarming tray 111 is suitable for dry recovery of the high-flux cell cryopreservation pipe and is electrically connected with the numerical control device 21 through a connector device.
The temperature control temperature sensor 1113 and the rewarming monitoring temperature sensor 1114 comprise a contact temperature sensor and a non-contact temperature sensor, the contact temperature sensor comprises a thermistor sensor, a thermocouple string sensor, a digital sensor, a platinum sensor and the like, and the non-contact temperature sensor comprises a thermopile array non-contact temperature sensor, an infrared temperature measuring probe temperature sensor, an infrared thermal imager temperature sensor and the like.
The double-work-recovery thermal cover 121 comprises a thermal cover frame 1211 and a second heating body 1212, the thermal cover frame 1211 is made of metal, the second heating body 1212 is located in the thermal cover frame 1211, the second heating body 1212 is an electric heating plate having two heating functions of conduction heating and far infrared radiation heating, the electric heating plate includes a carbon crystal electric heating plate, a carbon fiber electric heating plate, and the like, the second heating body 1212 is fixedly connected with the thermal cover frame 1211, the thermal cover frame 1211 is provided with a second temperature control temperature sensor 1213 capable of monitoring the temperature of the second heating body 1212 in real time, the number of the second temperature control temperature sensors 1213 is 1-6, the second heating body 1212 is provided with a through hole, a second temperature recovery monitoring temperature sensor 1214 is arranged in the through hole, the temperature of the cell cryopreservation bag can be monitored in real time, the number of the second temperature recovery monitoring temperature sensors 1214 is 1-6, the working temperature range of the double-work-recovery thermal cover 121 is 10 ℃ -70 ℃, and can be, the double-function heat recovery cover 121 is painted with a cell freezing bag or a cell freezing tube placing position guide line.
The double-work rewarming thermal cover 121 can be flat and is suitable for dry recovery of the cell cryopreservation bag, the double-work rewarming thermal cover 121 can also be provided with a plurality of cryopreservation pipe groove cavities, the cryopreservation pipe groove cavities are long and rectangular, the cross sections of the cryopreservation pipe groove cavities are semicircular and are suitable for dry recovery of a high-flux cell cryopreservation pipe, the cryopreservation pipe groove cavities of the double-work rewarming thermal cover 121 and the cryopreservation pipe groove cavities of the double-work rewarming tray 111 are correspondingly arranged, and the double-work rewarming thermal cover 121 is electrically connected with the numerical control device 21 through a connector device.
The buffer device 122 is a hydraulic buffer, a spring buffer or a damping buffer, and the damping buffer includes an air damping buffer, a gear damping buffer, a rack damping buffer, and a cylindrical damping buffer.
As shown in fig. 5, the bottom-supported kneading and mixing device includes a power source 31 and a power bracket 32, the power source 31 is fixedly connected to the bottom of the base 2, the power source 31 includes a servo motor 311 and a motor fixing frame 312, an eccentric wheel 313 is arranged at the motor shaft end of the servo motor 311, the number of the servo motor 311 is 2 or 4, the number of the power bracket 32 is 4, the number of the eccentric wheels 313 is 4, the size and the specification of the 4 eccentric wheels 313 are the same, the initial eccentric distance of the 4 eccentric wheels 313 in the vertical direction can be respectively adjusted during installation, the power bracket 32 movably connects the eccentric wheels 313 and the double-function double-temperature tray 111 through a ball-and-socket structure, the operation of the servo motor 311 is controlled by the numerical control device 21, and the bottom-supported kneading and mixing device 3 can drive the double-function double-temperature tray 111 to realize bottom-.
In the above, the bottom-supported kneading and mixing device may further comprise 4 electric fast hydraulic push rods, electric push rods, etc., which are movably connected to the dual-power rewarming tray 111, and the operation of the electric fast hydraulic push rods, the electric push rods, etc. is controlled by the numerical control device 21.
The communication device 22 can perform data exchange, data storage and data query with the internet or a local area network through wired or wireless networking.
The instrument device for the anhydrous dry-type cell resuscitation of embodiment 1 is characterized in that the resuscitation chamber and the base are fixedly connected integrally, the double-work rewarming tray is arranged in the resuscitation chamber, the bottom-supported kneading and mixing device can drive the double-work rewarming tray to perform bottom-supported kneading, oscillating and mixing movement, and the double-work rewarming tray moves in the resuscitation chamber and is a built-in mixing working mode.
The method of operation of an apparatus for the anhydrous dry resuscitation of cells of example 1, comprising the steps of:
(1) starting an instrument, setting the temperature required by recovery, selecting a recovery working mode, starting a preheating function, preheating a double-work rewarming tray and a double-work rewarming hot cover, starting to rise the temperature of the double-work rewarming tray and the double-work rewarming hot cover, emitting far infrared rays, after preheating, placing a cell cryopreservation bag or a cell cryopreservation pipe on the double-work rewarming tray, covering and fixing a recovery cabin cover, clamping the cell cryopreservation bag or the cell cryopreservation pipe by the double-work rewarming tray and the double-work rewarming hot cover under the action of a buffer device between the double-work rewarming hot cover and the recovery cabin cover, starting cell recovery, transferring heat to the cell cryopreservation bag or the cell cryopreservation pipe by two heat conduction modes of conduction and radiation at the same time by the double-work rewarming tray and the double-work rewarming hot cover, starting to work by a bottom support type mixing device, and starting to work by 4 eccentric wheels under the regulation and control of a numerical control device, the operation speeds are different, the double-work rewarming tray, the cell cryopreservation bag or the cell cryopreservation tube and the double-work rewarming hot cover are driven to perform bottom-supported kneading oscillation blending movement together, the force applied by the blending movement can be detected by a pressure sensor between the double-work rewarming hot cover and the resuscitation cabin cover and is transmitted to a numerical control device, the operation of a power source is intelligently fed back and adjusted, and the cell cryopreservation bag or an instrument is prevented from being damaged due to excessive force;
(2) the temperature of the double-power rewarming tray and the double-power rewarming hot cover is monitored at any time through the temperature control temperature sensor, detection data are transmitted to the numerical control device, the numerical control device controls the heating process of the double-power rewarming tray and the double-power rewarming hot cover, and the temperature is intelligently maintained at a set temperature;
(3) the rewarming monitoring temperature sensor detects whether the current temperature of the cell cryopreservation bag or the cell cryopreservation tube is matched with the set rewarming target temperature, the matching stability determination time is not more than 5 seconds, if the current temperature is lower than the rewarming target temperature, the heating rewarming state is started and maintained, and if the current temperature is higher than the rewarming target temperature, the heating is immediately stopped;
(4) when the cell freezing and storing device is in a heating mode, the double-power heating tray and the double-power temperature-restoring hot cover are in a heating state, the temperature of the tray body and the temperature of the hot cover are increased, far infrared rays are emitted, and heat is transferred to the cell freezing and storing bag or the cell freezing and storing pipe through two heat conduction modes of conduction and radiation to restore the temperature;
(5) when the rewarming monitoring temperature sensor detects that the temperature of the cell cryopreservation bag exceeds 0-8 ℃, the heating mode is closed after the cell cryopreservation bag or ice blocks in the cell cryopreservation pipe are completely melted, the double-power rewarming tray and the double-power rewarming heating cover stop working, the exhaust fan is started at the same time, air is sucked from the ventilation openings of the bottom, the side wall and the rear wall of the base, rises along the gap in the resuscitation chamber, is finally exhausted from the exhaust fan, takes away the heat of the double-power rewarming tray and the double-power rewarming heating cover, and the bottom-supported kneading and mixing device continuously carries out bottom-supported kneading oscillation and mixing movement;
(6) the cell thawing work of the cell freezing bag or the cell freezing tube is completed, and the operator is reminded to take away the thawed cell freezing bag or the cell freezing tube in time through acousto-optic signals such as indicator light flickering and loudspeaker sounding.
Example 2:
the utility model provides an instrument device for anhydrous dry-type of cell is come back to life or consciousness, includes and comes back to life or consciousness cabin 1, base 2 and collet formula mixing device 3, collet formula mixing device 3 is the three-dimensional mixing device of collet formula, comes back to life or consciousness cabin 1 and the split type design of base 2, and the three-dimensional mixing device of collet formula is installed in base 2, and it is back to life or consciousness cabin 1 and base 2 through the power support 32 swing joint of the three-dimensional mixing device of collet formula, and the three-dimensional mixing device of collet formula has supported whole room 1 that comes back to life or consciousness through.
The resuscitation cabin 1 comprises a resuscitation cabin body 11 and a resuscitation cabin cover 12, the resuscitation cabin body 11 is movably connected with the resuscitation cabin cover 12, the resuscitation cabin cover 12 can cover the resuscitation cabin body 11 to form a closed resuscitation space, a double-work rewarming tray 111 is arranged in the resuscitation cabin body 11, the double-work rewarming tray 111 can be detached and replaced, a double-work rewarming thermal cover 121 is arranged in the resuscitation cabin cover 12, the double-work rewarming thermal cover 121 is movably connected with the resuscitation cabin cover 12, a buffer device 122 and a pressure sensing device 124 are arranged between the double-work rewarming thermal cover 121 and the resuscitation cabin cover 12, and the double-work rewarming thermal cover 121 can be detached and replaced.
And a numerical control device, a communication device and a touch screen are also arranged in the base 2.
The double-work rewarming tray 111 comprises a tray frame 1111 and a first heating body 1112, the tray frame 1111 is made of metal, the first heating body 1112 is positioned in the tray frame 1111, the first heating body 1112 is an electric heating plate with two heating functions of conduction heating and far infrared radiation heating, the electric heating plate comprises a carbon crystal electric heating plate, a carbon fiber electric heating plate and the like, the first heating body 1112 is fixedly connected with the tray frame 1111, a temperature control temperature sensor is arranged in the tray frame 1111 and can monitor the temperature of the first heating body 1112 in real time, the number of the temperature control temperature sensors is 1-6, preferably 2, a through hole is formed in the center of the first heating body 1112, a rewarming monitoring temperature sensor 1114 is arranged in the through hole and can monitor the temperature of the cell cryopreservation bag in real time, the number of the rewarming monitoring temperature sensors 1114 is 1-6, preferably 2, the working temperature range of the double-work rewarming tray 111 is 10-, the setting can be carried out through the touch screen, and the placing position guide line of the cell cryopreservation bag or the cell cryopreservation tube is drawn on the double-function rewarming tray 111.
The double-function rewarming tray 111 can be a flat tray and is suitable for dry recovery of cell cryopreservation bags, a plurality of cryopreservation pipe groove cavities can be further formed in the double-function rewarming tray 111, the cryopreservation pipe groove cavities are long and rectangular, the cross sections of the cryopreservation pipe groove cavities are semicircular and are suitable for dry recovery of high-flux cell cryopreservation pipes, and the double-function rewarming tray 111 is electrically connected with the recovery cabin body 11 through a connector device.
The temperature control temperature sensor and the rewarming monitoring temperature sensor comprise a contact temperature sensor and a non-contact temperature sensor, the contact temperature sensor comprises a thermistor sensor, a thermocouple string sensor, a digital sensor, a platinum sensor and the like, and the non-contact temperature sensor comprises a thermopile array non-contact temperature sensor, an infrared temperature measuring probe temperature sensor, an infrared thermal imager temperature sensor and the like.
The double-work heat recovery cover 112 comprises a heat cover frame 1121 and a second heating body 1122, the heat cover frame 1121 is made of metal, the second heating body 1122 is located in the heat cover frame 1121, the second heating body 1122 is an electric heating plate having two heating functions of conduction heating and far infrared radiation heating, the electric heating plate comprises a carbon crystal electric heating plate, a carbon fiber electric heating plate and the like, the second heating body 1122 is fixedly connected with the heat cover frame 1121, the heat cover frame 1121 is provided with a temperature control temperature sensor 1123 which can monitor the temperature of the second heating body 1122 in real time, the number of the temperature control temperature sensors 1123 is 1-6, preferably 2, the working temperature range of the double-work heat recovery cover 112 is 10-70 ℃, the setting can be carried out through a touch screen, and a placing position finger lead of a cell cryopreservation bag or a cell cryopreservation tube is drawn on the double-work heat recovery cover 112.
The double-work rewarming hot cover 112 can be flat and is suitable for dry recovery of the cell cryopreservation bag, the double-work rewarming hot cover 112 can also be provided with a plurality of cryopreservation pipe groove cavities, the cryopreservation pipe groove cavities are long rectangular, the cross sections of the cryopreservation pipe groove cavities are semicircular and are suitable for dry recovery of a high-flux cell cryopreservation pipe, the cryopreservation pipe groove cavities of the double-work rewarming hot cover 112 correspond to the cryopreservation pipe groove cavities of the double-work rewarming tray 111 one by one, and the double-work rewarming hot cover 112 is electrically connected with the recovery cabin body 11 through a connector device.
The buffer device 122 includes a hydraulic buffer, a spring buffer, and a damping buffer, and the damping buffer includes an air damping buffer, a gear damping buffer, a rack damping buffer, and a cylindrical damping buffer.
The pressure sensor is a piezoresistive pressure sensor, a ceramic pressure sensor, a diffused silicon pressure sensor, a sapphire pressure sensor or a piezoelectric pressure sensor.
The bottom-supported three-dimensional blending device comprises a power source 31 and a power support 32, the power source 31 is arranged in a base 2 and is fixedly connected to the bottom in the base 2, the power source 31 comprises a servo motor 311, a motor fixing frame 312 and eccentric wheels 313, the eccentric wheels 313 are arranged at one end or two ends of the servo motor 311, the number of the servo motor 311 is 2 or 4, the number of the power support 32 is 4, the number of the eccentric wheels 313 is 4, the power support 32 movably connects the eccentric wheels 313 and the resuscitation cabin 1 through 4 ball-and-socket type connection points, the operation of the servo motor 311 is controlled by a numerical control device, the initial working angles of the 4 eccentric wheels 313 are different under the control of the numerical control device, the operation speeds are different, and the bottom-supported three-dimensional blending device can drive the resuscitation cabin 1 to integrally realize complex three-dimensional bumpy.
The bottom-supported three-dimensional mixing device can also be an electric quick hydraulic ejector rod, an electric hydraulic push rod, an electric push rod and the like, the number of the electric quick hydraulic ejector rod, the electric hydraulic push rod, the electric push rod and the like is 4, the electric quick hydraulic ejector rod, the electric hydraulic push rod, the electric push rod and the like are respectively movably connected with the resuscitation cabin through 4 connecting points, the operation of the electric quick hydraulic ejector rod, the electric hydraulic push rod, the electric push rod and the like is regulated and controlled by a numerical control device, the operation speed and the operation height are different, and the bottom-supported three-dimensional mixing device can drive.
The communication device can be connected with the internet or a local area network through a wired network or a wireless network to perform data exchange, data storage and data query.
The instrument device for the anhydrous dry cell resuscitation of embodiment 2 is characterized in that the resuscitation cabin and the base are arranged in a split mode, the base-mounted three-dimensional mixing device body is arranged in the base, the resuscitation cabin and the base are movably connected through a power support of the base-mounted three-dimensional mixing device, the base-mounted three-dimensional mixing device can drive the resuscitation cabin to integrally perform complex three-dimensional bumping oscillation type mixing motion, the resuscitation cabin moves to drive the double-function rewarming tray inside the resuscitation cabin to move, and the instrument device is an external mixing working mode.
The method of operation of an apparatus for the anhydrous dry resuscitation of cells of embodiment 2, comprising the steps of:
(1) starting an instrument, selecting a recovery mode, starting a preheating function, starting preheating by the aid of the double-work rewarming tray and the double-work rewarming hot cover, starting temperature rise of the double-work rewarming tray and the double-work rewarming hot cover, emitting far infrared rays, after preheating is finished, placing the cell cryopreservation bag or the cell cryopreservation pipe on the double-work rewarming tray, covering and fixing a recovery cabin cover, starting cell recovery, transmitting heat to the cell cryopreservation bag or the cell cryopreservation pipe by the aid of the double-work rewarming tray and the double-work rewarming hot cover in a conduction and radiation mode, starting a bottom-mounted three-dimensional blending device to work, and driving the whole recovery cabin to perform complex three-dimensional bumping oscillation type blending movement;
(2) the temperature of the double-power rewarming tray and the double-power rewarming hot cover is monitored at any time through the temperature control temperature sensor, detection data are transmitted to the numerical control device, the numerical control device controls the heating process of the double-power rewarming tray and the double-power rewarming hot cover, and the temperature is intelligently maintained at a set temperature;
(3) the rewarming monitoring temperature sensor monitors whether the current temperature of the cell cryopreservation bag or the cell cryopreservation tube is matched with the set rewarming target temperature or not in real time, the matching stability determination time is not more than 5 seconds, if the current temperature is lower than the rewarming target temperature, a heating rewarming mode is started and maintained, and if the current temperature is higher than the rewarming target temperature, heating is stopped immediately;
(4) when the cell freezing and storing device is in a heating mode, the double-power heating tray and the double-power temperature-restoring heating cover are in a heating state, the temperature of the tray body and the temperature of the heating cover are increased, far infrared rays are emitted, and heat is transferred to the cell freezing and storing bag or the cell freezing and storing pipe in a conduction mode and a radiation mode to restore the temperature;
(5) when the rewarming monitoring temperature sensor detects that the temperature of the cell cryopreservation bag exceeds 0-8 ℃, the heating mode is closed after the ice blocks in the cell cryopreservation bag or the cell cryopreservation tube are completely melted, the double-function rewarming tray and the double-function rewarming heating cover stop working, the cell recovery work of the cell cryopreservation bag or the cell cryopreservation tube is completed, the recovery cabin cover is automatically opened, and an operator is reminded to take away the recovered cell cryopreservation bag or the cell cryopreservation tube in time through acousto-optic signals such as indicator light flickering, loudspeaker sounding and the like.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, similar modifications and improvements can be made without departing from the inventive concept of the present invention, and these are also considered to be within the protection of the present invention.
Claims (8)
1. An instrument device for anhydrous dry resuscitation of cells, characterized by: the recovery device comprises a recovery cabin (1), a base (2) and a bottom-mounted blending device (3), wherein the recovery cabin (1) is located above the base (2), the recovery cabin (1) comprises a recovery cabin body (11) and a recovery cabin cover (12) movably connected to the recovery cabin body (11), the recovery cabin cover (12) can cover the recovery cabin body (11) to form a closed recovery space, a double-work temperature-recovery tray (111) is arranged in the recovery cabin body (11), and a double-work temperature-recovery heat cover (121) is arranged in the recovery cabin cover (12); the bottom-supported blending device (3) is arranged in the base (2) and is connected with the double-work rewarming tray (111) in the resuscitation cabin body (11).
2. An instrument device for anhydrous dry resuscitation of cells according to claim 1, wherein: the double-work rewarming tray (111) is detachable and replaceable, a buffer device (122) and a pressure sensor (124) are arranged between the double-work rewarming thermal cover (121) and the resuscitation cabin cover (12), the double-work rewarming thermal cover (121) is movably connected with the resuscitation cabin cover (12) through the buffer device (122) and the pressure sensor (124), the double-work rewarming thermal cover (121) is detachable and replaceable, and an exhaust fan (123) is further arranged in the resuscitation cabin cover (12).
3. An instrument device for anhydrous dry resuscitation of cells according to claim 2, wherein: air is followed the vent of base (2) bottom, lateral wall and back wall inhales, rises along the inside space of recovery cabin (1) cavity, by exhaust fan (123) discharges, base (2) are equipped with numerical control device (21), communication device (22) and touch screen (23).
4. An instrument device for anhydrous dry resuscitation of cells according to claim 2, wherein: the double-power rewarming tray (111) comprises a tray frame (1111) and a first heating body (1112), wherein the tray frame (1111) is made of metal, the first heating body (1112) is arranged in the tray frame (1111), the first heating body (1112) is an electric heating plate with two heating functions of conduction heating and far infrared radiation heating, the first heating body (1112) is fixedly connected with the tray frame (1111), the tray frame (1111) is provided with a first temperature control temperature sensor (1113), the number of the first temperature control temperature sensors (1113) is 1-6, a through hole is formed in the first heating body (1112), a first rewarming monitoring temperature sensor (1114) is arranged in the through hole, the temperature of a cell cryopreservation bag can be monitored in real time, and the number of the first rewarming monitoring temperature sensors (1114) is 1-6;
the working temperature range of the double-function rewarming tray (111) is 10-70 ℃, and a guide line for placing the cell cryopreservation bag or the cell cryopreservation tube is drawn on the double-function rewarming tray (111).
5. An instrument device for anhydrous dry resuscitation of cells according to claim 2, wherein: the double-work heat recovery cover (121) comprises a heat cover frame (1211) and a second heating body (1212), the heat cover frame (1211) is made of metal, the second heating body (1212) is located in the heat cover frame (1211), the second heating body (1212) is an electric heating plate which has two heating functions of conduction heating and far infrared radiation heating, the second heating body (1212) is fixedly connected with the heat cover frame (1211), the heat cover frame (1211) is provided with second temperature control temperature sensors (1213), the number of the second temperature control temperature sensors (1213) is 1-6, the second heating body (1212) is provided with through holes, second heat recovery monitoring temperature sensors (1214) are arranged in the through holes, the number of the second heat recovery monitoring temperature sensors (1214) is 1-6, the working temperature range of the double-work heat recovery cover (121) is 10-70 ℃, the double-function heat recovery cover (121) is painted with a cell freezing bag or a cell freezing tube placing position guide line.
6. An instrument device for anhydrous dry resuscitation of cells according to claim 3, wherein: bottom-supported mixing device (3) includes power supply (31) and power support (32), power supply (31) fixed connection in bottom in base (2), power supply (31) are including servo motor (311) and motor mount (312), the motor shaft end of servo motor (311) is equipped with eccentric wheel (313), the figure of servo motor (311) is 2 or 4, the figure of power support (32) is 4, the figure of eccentric wheel (313) is 4, 4 the size specification of eccentric wheel (313) is the same, can adjust 4 respectively during the installation the initial eccentricity of eccentric wheel (313) vertical direction, power support (32) will eccentric wheel (313) with double work rewarming tray (111) swing joint, the operation of servo motor (311) is received the regulation and control of numerical control device (21), the bottom-supported type blending device (3) can drive the double-function rewarming tray (111) to realize bottom-supported type kneading, oscillating and blending movement.
7. An instrument device for anhydrous dry resuscitation of cells according to claim 3, wherein: the bottom-supporting type blending device (3) can also be an electric quick hydraulic ejector rod, an electric hydraulic push rod or an electric push rod, the number of the electric quick hydraulic ejector rod, the electric hydraulic push rod, the electric push rod or the like is 4, the electric quick hydraulic ejector rod, the electric hydraulic push rod, the electric push rod or the like are respectively movably connected with the double-power rewarming tray (111), and the operation of the electric quick hydraulic ejector rod, the electric hydraulic push rod, the electric push rod or the like is controlled by the numerical control device (21).
8. An instrument device for anhydrous dry resuscitation of cells according to claim 3, wherein: the communication device (22) can perform data exchange, data storage and data query with the Internet or a local area network through wired or wireless networking.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110172400A (en) * | 2019-06-26 | 2019-08-27 | 赛慕特生物工程（上海）有限公司 | A kind of apparatus for the anhydrous dry type recovery of cell |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110172400A (en) * | 2019-06-26 | 2019-08-27 | 赛慕特生物工程（上海）有限公司 | A kind of apparatus for the anhydrous dry type recovery of cell |
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Effective date of registration: 20240906 Address after: 236000 Factory Building A-19, Intelligent Manufacturing Industrial Park, Yingquan District Economic Development Zone, Fuyang City, Anhui Province Patentee after: Anhui Bairuinuokang Pharmaceutical Technology Co.,Ltd. Country or region after: China Address before: 200000 Room 209, 2nd Floor, Building 2, No. 58 Xiangke Road, Pudong New Area Pilot Free Trade Zone, Shanghai Patentee before: THERMBEST BIOENGINEERING (SHANGHAI) CO.,LTD. Country or region before: China |