CN117402721A - A detection device and detection method for multi-color fluorescence detection - Google Patents
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Abstract
Description
技术领域Technical field
本发明涉及医疗器械技术领域，尤其涉及一种多色荧光检测的检测装置及检测方法。The present invention relates to the technical field of medical devices, and in particular to a detection device and detection method for multi-color fluorescence detection.
背景技术Background technique
分子诊断是体外诊断的重要分支。PCR(Polymerase Chain Reaction聚合酶链式反应)技术是分子诊断技术中心应用最为广泛的技术之一。PCR技术是体外酶促合成特异DNA片段的一种方法，由高温变性、低温退火、适温延伸等反应组成1个周期，循环进行，使目的DNA得以迅速扩增。Molecular diagnostics is an important branch of in vitro diagnostics. PCR (Polymerase Chain Reaction) technology is one of the most widely used technologies in the Molecular Diagnostic Technology Center. PCR technology is a method for enzymatic synthesis of specific DNA fragments in vitro. It consists of a cycle of high-temperature denaturation, low-temperature annealing, and temperature-appropriate extension reactions, which are carried out in cycles to rapidly amplify the target DNA.
PCR反应体系中加入特异性的荧光探针，反应体系的荧光强度与DNA片段的扩增倍数成正比。在每一个PCR循环中实时采集荧光强度，可得到DNA片段的扩增强度，实现PCR检测过程的实时定量。通过多重荧光的方法可同时检测多个被测靶标(样本)，提高DNA片段检测的效率。A specific fluorescent probe is added to the PCR reaction system, and the fluorescence intensity of the reaction system is proportional to the amplification multiple of the DNA fragment. By collecting fluorescence intensity in real time during each PCR cycle, the amplification intensity of DNA fragments can be obtained to achieve real-time quantification of the PCR detection process. Multiple targets (samples) can be detected simultaneously through multiple fluorescence methods, improving the efficiency of DNA fragment detection.
中国发明专利申请CN114181823A公开了一种多温控模块异步可选配通道的PCR仪及其检测方法，多色荧光检测模块采用多个单色荧光检测通道并联，扩增孔板到荧光检测模块之间通过光纤传输，荧光检测模组通过皮带导轨带动做直线运动实现全部光纤的扫描。荧光检测模块采用分色镜的方案，激发滤光片波长为L1,分色镜波长为L2，发射滤光片波长为L3，L1<L2<L3。分色镜对波长小于L2的光反射，对波长大于L2的光透射。发光元件发出的光经激发滤光片过滤，在分色镜上反射，射出检测模块。检测的光透射分色镜，经发射滤光片过滤，照在感光元件上，进行光强检测。每个检测通道到被测试管之间只有一根光纤，光纤内激发光和发射光双向传输。Chinese invention patent application CN114181823A discloses a PCR instrument with multiple temperature control modules and asynchronous optional channels and its detection method. The multi-color fluorescence detection module uses multiple single-color fluorescence detection channels in parallel, and the amplification well plate is connected to the fluorescence detection module. are transmitted through optical fibers, and the fluorescence detection module is driven by a belt guide rail to perform linear motion to scan all optical fibers. The fluorescence detection module adopts a dichroic mirror solution. The excitation filter wavelength is L1, the dichroic mirror wavelength is L2, and the emission filter wavelength is L3, L1<L2<L3. The dichroic mirror reflects light with a wavelength smaller than L2 and transmits light with a wavelength larger than L2. The light emitted by the light-emitting element is filtered by the excitation filter, reflected on the dichroic mirror, and emitted out of the detection module. The detected light is transmitted through the dichroic mirror, filtered by the emission filter, and illuminated on the photosensitive element for light intensity detection. There is only one optical fiber between each detection channel and the tube being tested, and the excitation light and emitted light are transmitted in both directions within the optical fiber.
中国实用新型专利CN209065925U公开了一种多通道荧光定量PCR仪，扩增孔板为96孔，荧光检测模块为多通道，圆形布置，通过电机带动旋转，扫描所有试管孔。激发管和探测管为两个分开的管，每个管到被测试管之间都有一根光纤，因此要检测一个试管的单色荧光通道，需要2根光纤，一根单向走激发光，一根单向走发射光。Chinese utility model patent CN209065925U discloses a multi-channel fluorescence quantitative PCR instrument with a 96-well amplification well plate and a multi-channel fluorescence detection module in a circular arrangement. It is driven by a motor to rotate and scan all test tube holes. The excitation tube and the detection tube are two separate tubes. There is an optical fiber between each tube and the test tube. Therefore, to detect the monochromatic fluorescence channel of a test tube, two optical fibers are needed, one for unidirectional excitation light. A one-way beam emits light.
现有技术中多色荧光检测均需要设置多组光纤，利用多组光纤传输激发光和发射光，光纤传输成本高、光强有损失，并且现有技术中多色荧光检测装置多个单色检测通量并联，体积大。In the existing technology, multi-color fluorescence detection requires multiple sets of optical fibers to transmit excitation light and emitted light. The optical fiber transmission cost is high and the light intensity is lost, and the multi-color fluorescence detection device in the existing technology has multiple single-color The detection flux is connected in parallel and the volume is large.
发明内容Contents of the invention
本发明提供了一种多色荧光检测的检测装置及检测方法，以解决现有多色荧光检测装置光纤传输成本高、光强有损失，多个单色检测通量并联，体积大的技术问题。The invention provides a detection device and a detection method for multi-color fluorescence detection to solve the technical problems of high optical fiber transmission cost, loss of light intensity, multiple single-color detection fluxes in parallel, and large volume of existing multi-color fluorescence detection devices. .
本发明提供的技术方案如下：The technical solutions provided by the invention are as follows:
本发明的一个目的在于提供一种多色荧光检测的检测装置，所述检测装置至少包括温控模块和荧光检测模块；An object of the present invention is to provide a detection device for multi-color fluorescence detection, which detection device at least includes a temperature control module and a fluorescence detection module;
所述温控模块包括阵列的多个试管座，每一个所述试管座用于放置一个试管；每一个所述试管座开设一个检测孔，多个所述试管座的多个所述检测孔处于同一直线；The temperature control module includes a plurality of test tube holders in an array, each test tube holder is used to place a test tube; each test tube holder is provided with a detection hole, and the detection holes of the plurality of test tube holders are at same straight line;
所述荧光检测模块至少包括发光组件、测光组件和出光孔；所述荧光检测模块布置于所述温控模块一侧，所述荧光检测模块被配置为：沿多个所述检测孔所处的直线方向往复运动，使所述出光孔依次对准每一个所述试管座的检测孔；The fluorescence detection module at least includes a light-emitting component, a photometric component and a light outlet; the fluorescence detection module is arranged on one side of the temperature control module, and the fluorescence detection module is configured to: along a plurality of the detection holes Reciprocating movement in the linear direction, so that the light outlet holes are aligned with the detection holes of each of the test tube holders;
所述发光组件至少包括多色发光元件和发光多通滤光片；所述测光组件至少包括测光多通滤光片和测光元件；The light-emitting component at least includes a multi-color light-emitting element and a light-emitting multi-pass filter; the photometric component at least includes a photometric multi-pass filter and a photometric element;
在所述发光多通滤光片和所述测光多通滤光片之间倾斜45度角布置一分色镜；A dichroic mirror is arranged at an angle of 45 degrees between the luminescent multi-pass filter and the photometric multi-pass filter;
所述发光组件和所述测光组件的光轴垂直，并且发光多通滤光片与所述出光孔位于所述分色镜的同一侧，并且所述发光组件的光轴与所述出光孔的轴线处于同一直线；The optical axes of the light-emitting component and the photometric component are perpendicular, and the light-emitting multi-pass filter and the light exit hole are located on the same side of the dichroic mirror, and the optical axis of the light emitting component is aligned with the light exit hole. axes are in the same straight line;
其中，所述多色发光元件的波谱范围数与所述发光多通滤光片的通带数相等，所述多色发光元件的波谱范围数与所述测光多通滤光片的通带数相等；所述测光多通滤光片的通带数与所述分色镜的通带数相等；Wherein, the number of spectral ranges of the multi-color light-emitting element is equal to the number of pass bands of the light-emitting multi-pass filter, and the number of spectral ranges of the multi-color light-emitting element is equal to the pass band number of the photometric multi-pass filter. The numbers are equal; the passband number of the photometric multi-pass filter is equal to the passband number of the dichroic mirror;
其中，所述多色发光元件的一个波谱范围对应所述发光多通滤光片的一个通带；Wherein, a spectrum range of the multi-color light-emitting element corresponds to a pass band of the light-emitting multi-pass filter;
其中，所述发光多通滤光片的每一个通带与所述测光多通滤光片的每一个通带彼此不重叠；Wherein, each passband of the luminescent multipass filter and each passband of the photometric multipass filter do not overlap with each other;
其中，所述发光多通滤光片的所有通带之间彼此不重叠，所述测光多通滤光片的所有通带之间彼此不重叠；Wherein, all passbands of the luminescent multipass filter do not overlap with each other, and all passbands of the photometric multipass filter do not overlap with each other;
其中，所述分色镜的一个通带对应所述测光多通滤光片的一个通带，并且所述发光多通滤光片的每一个通带与所述分色镜的每一个通带彼此不重叠；Wherein, one passband of the dichroic mirror corresponds to one passband of the photometric multipass filter, and each passband of the luminescent multipass filter is connected to each passband of the dichroic mirror. The bands do not overlap each other;
其中，所述分色镜的所有通带之间彼此不重叠。Wherein, all passbands of the dichroic mirror do not overlap with each other.
在一个较佳的实施例中，所述检测装置还包括支架，所述支架上固定导轨、主动轮和从动轮，所述主动轮和所述从动轮之间环绕布置皮带；In a preferred embodiment, the detection device further includes a bracket, a guide rail, a driving wheel and a driven wheel are fixed on the bracket, and a belt is arranged around the driving wheel and the driven wheel;
所述皮带与所述荧光检测模块固定，所述荧光检测模块与所述导轨滑动安装；The belt is fixed to the fluorescence detection module, and the fluorescence detection module is slidably installed on the guide rail;
所述主动轮驱动所述皮带运动，所述皮带带动所述荧光检测模块沿多个所述检测孔所处的直线方向往复运动。The driving wheel drives the belt to move, and the belt drives the fluorescence detection module to reciprocate along the linear direction where the plurality of detection holes are located.
在一个较佳的实施例中，所述温控模块还包括加热板、温控元件和散热器；In a preferred embodiment, the temperature control module also includes a heating plate, a temperature control element and a radiator;
所述加热板置于所述温控元件上，所述温控元件置于所述散热器上，多个所述试管座阵列于所述加热板上。The heating plate is placed on the temperature control element, the temperature control element is placed on the radiator, and a plurality of test tube holders are arrayed on the heating plate.
在一个较佳的实施例中，所述发光组件还包括发光透镜，所述发光透镜置于所述多色发光元件和所述发光多通滤光片之间，用于将所述多色发光元件发出的不同波谱范围的激发光传输至所述发光多通滤光片；In a preferred embodiment, the light-emitting component further includes a light-emitting lens. The light-emitting lens is placed between the multi-color light-emitting element and the light-emitting multi-pass filter for converting the multi-color light-emitting element. The excitation light in different spectral ranges emitted by the element is transmitted to the luminescent multi-pass filter;
所述测光组件还包括测光透镜，所述测光透镜置于所述测光多通滤光片和测光元件之间，用于将所述测光多通滤光片分离的不同波长的荧光传输至所述测光元件；The photometric component also includes a photometric lens, which is placed between the photometric multi-pass filter and the photometric element, and is used to separate different wavelengths of the photometric multi-pass filter. The fluorescence is transmitted to the photometric element;
所述荧光检测模块还包括前压盖，所述前压盖上开设所述出光孔，所述前压盖内安装出光透镜，所述出光透镜正对所述出光孔。The fluorescence detection module also includes a front gland, the light outlet hole is opened on the front gland, a light outlet lens is installed in the front gland, and the light outlet lens faces the light outlet hole.
在一个较佳的实施例中，所述试管内置多种不同染料和被测靶标，每种染料的荧光检测强度与被测靶标的浓度正相关。In a preferred embodiment, the test tube contains a plurality of different dyes and the target to be measured, and the fluorescence detection intensity of each dye is positively related to the concentration of the target to be measured.
在一个较佳的实施例中，发光多通滤光片的一个通带对应一种所述染料的激发波长；测光多通滤光片的一个通带对应一种所述染料的荧光波长。In a preferred embodiment, one passband of the luminescent multipass filter corresponds to one excitation wavelength of the dye; one passband of the photometric multipass filter corresponds to one fluorescence wavelength of the dye.
本发明的另一个方面在于提供一种多色荧光检测的检测方法，所述检测方法包括如下方法步骤：Another aspect of the present invention is to provide a detection method for multi-color fluorescence detection. The detection method includes the following method steps:
多色发光元件发出不同波谱范围的激发光；Multicolor light-emitting elements emit excitation light in different spectral ranges;
发光多通滤光片，将所述多色发光元件的不同波谱范围的激发光，分离出不同波长的激发单色光；A luminescent multi-pass filter, which separates the excitation light in different spectral ranges of the multi-color light-emitting element into excitation monochromatic light of different wavelengths;
分色镜，将不同波长的激发单色光垂直反射至出光孔，并经试管座的检测孔射入试管内，激发所述试管内的多种不同染料产生不同波长的荧光；The dichroic mirror vertically reflects the excitation monochromatic light of different wavelengths to the light outlet hole, and injects it into the test tube through the detection hole of the test tube holder, exciting a variety of different dyes in the test tube to produce fluorescence of different wavelengths;
不同波长的荧光反射至所述分色镜，所述分色镜将不同波长的荧光传输至测光多通滤光片；Fluorescence of different wavelengths is reflected to the dichroic mirror, and the dichroic mirror transmits fluorescence of different wavelengths to the photometric multi-pass filter;
所述测光多通滤光片，分离不同波长的荧光，并将不同波长的荧光传输至测光元件；The photometric multi-pass filter separates fluorescence of different wavelengths and transmits fluorescence of different wavelengths to the photometric element;
其中，所述多色发光元件的波谱范围数与所述发光多通滤光片的通带数相等，所述多色发光元件的波谱范围数与所述测光多通滤光片的通带数相等；所述测光多通滤光片的通带数与所述分色镜的通带数相等；Wherein, the number of spectral ranges of the multi-color light-emitting element is equal to the number of pass bands of the light-emitting multi-pass filter, and the number of spectral ranges of the multi-color light-emitting element is equal to the pass band number of the photometric multi-pass filter. The numbers are equal; the passband number of the photometric multi-pass filter is equal to the passband number of the dichroic mirror;
其中，所述多色发光元件的一个波谱范围对应所述发光多通滤光片的一个通带；Wherein, a spectrum range of the multi-color light-emitting element corresponds to a pass band of the light-emitting multi-pass filter;
其中，所述发光多通滤光片的每一个通带与所述测光多通滤光片的每一个通带彼此不重叠；Wherein, each passband of the luminescent multipass filter and each passband of the photometric multipass filter do not overlap with each other;
其中，所述发光多通滤光片的所有通带之间彼此不重叠，所述测光多通滤光片的所有通带之间彼此不重叠；Wherein, all passbands of the luminescent multipass filter do not overlap with each other, and all passbands of the photometric multipass filter do not overlap with each other;
其中，所述分色镜的一个通带对应所述测光多通滤光片的一个通带，并且所述发光多通滤光片的每一个通带与所述分色镜的每一个通带彼此不重叠；Wherein, one passband of the dichroic mirror corresponds to one passband of the photometric multipass filter, and each passband of the luminescent multipass filter is connected to each passband of the dichroic mirror. The bands do not overlap each other;
其中，所述分色镜的所有通带之间彼此不重叠。Wherein, all passbands of the dichroic mirror do not overlap with each other.
在一个较佳的实施例中，所述多色发光元件和所述发光多通滤光片之间设置发光透镜，用于将所述多色发光元件发出的不同波谱范围的激发光传输至所述发光多通滤光片；In a preferred embodiment, a light-emitting lens is provided between the multi-color light-emitting element and the light-emitting multi-pass filter for transmitting excitation light in different spectral ranges emitted by the multi-color light-emitting element to the The luminescent multi-pass filter;
所述光多通滤光片和测光元件之间设置测光透镜，用于将所述测光多通滤光片分离的不同波长的荧光传输至所述测光元件。A photometric lens is disposed between the photometric multi-pass filter and the photometric element for transmitting fluorescence of different wavelengths separated by the photometric multi-pass filter to the photometric element.
在一个较佳的实施例中，所述试管内置多种不同染料和被测靶标，每种染料的荧光检测强度与被测靶标的浓度正相关。In a preferred embodiment, the test tube contains a plurality of different dyes and the target to be measured, and the fluorescence detection intensity of each dye is positively related to the concentration of the target to be measured.
在一个较佳的实施例中，发光多通滤光片的一个通带对应一种所述染料的激发波长；测光多通滤光片的一个通带对应一种所述染料的荧光波长。In a preferred embodiment, one passband of the luminescent multipass filter corresponds to one excitation wavelength of the dye; one passband of the photometric multipass filter corresponds to one fluorescence wavelength of the dye.
本发明上述技术方案，与现有技术相比至少具有如下有益效果：The above technical solution of the present invention has at least the following beneficial effects compared with the prior art:
本发明提供一种多色荧光检测的检测装置及检测方法，通过多色发光元件、发光多通滤光片、分色镜、测光多通滤光片实现单个荧光检测模块的多色荧光检测，并省去光纤传输，通过单个荧光检测模块进行扫描，布置紧凑，成本低。The invention provides a detection device and detection method for multi-color fluorescence detection, which realizes multi-color fluorescence detection of a single fluorescence detection module through multi-color light-emitting elements, luminescence multi-pass filters, dichroic mirrors and photometric multi-pass filters. , and eliminates the need for optical fiber transmission and scans through a single fluorescence detection module, which has a compact layout and low cost.
附图说明Description of the drawings
为了更清楚地说明本发明实施例中的技术方案，下面将对实施例描述中所需要使用的附图作简单地介绍，显而易见地，下面描述中的附图仅仅是本发明的一些实施例，对于本领域普通技术人员来讲，在不付出创造性劳动的前提下，还可以根据这些附图获得其他的附图。In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the description of the embodiments will be briefly introduced below. Obviously, the drawings in the following description are only some embodiments of the present invention. For those of ordinary skill in the art, other drawings can also be obtained based on these drawings without exerting creative efforts.
图1是本发明一种多色荧光检测的检测装置的整体结构示意图。Figure 1 is a schematic diagram of the overall structure of a detection device for multi-color fluorescence detection according to the present invention.
图2是本发明温控模块的结构示意图。Figure 2 is a schematic structural diagram of the temperature control module of the present invention.
图3是本发明荧光检测模块的结构示意图。Figure 3 is a schematic structural diagram of the fluorescence detection module of the present invention.
图4是本发明温控模块和荧光检测模块布置的截面图。Figure 4 is a cross-sectional view of the arrangement of the temperature control module and the fluorescence detection module of the present invention.
图5是本发明荧光检测模块的截面图。Figure 5 is a cross-sectional view of the fluorescence detection module of the present invention.
图6是本发明一个实施例中多色发光元件的波谱范围、发光多通滤光片的通带、测光多通滤光片的通带，以及分色镜的通带之间的关系示意图。6 is a schematic diagram of the relationship between the spectral range of the multicolor light-emitting element, the passband of the luminescent multipass filter, the passband of the photometric multipass filter, and the passband of the dichroic mirror in one embodiment of the present invention. .
具体实施方式Detailed ways
为使本发明实施例的目的、技术方案和优点更加清楚，下面将结合本发明实施例的附图，对本发明实施例的技术方案进行清楚、完整地描述。显然，所描述的实施例是本发明的一部分实施例，而不是全部的实施例。基于所描述的本发明的实施例，本领域普通技术人员在无需创造性劳动的前提下所获得的所有其他实施例，都属于本发明保护的范围。In order to make the purpose, technical solutions and advantages of the embodiments of the present invention more clear, the technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings of the embodiments of the present invention. Obviously, the described embodiments are some, but not all, of the embodiments of the present invention. Based on the described embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts fall within the scope of protection of the present invention.
除非另外定义，本发明使用的技术术语或者科学术语应当为本发明所属领域内具有一般技能的人士所理解的通常意义。本发明中使用的“第一”、“第二”以及类似的词语并不表示任何顺序、数量或者重要性，而只是用来区分不同的组成部分。同样，“一个”、“一”或者“该”等类似词语也不表示数量限制，而是表示存在至少一个。“包括”或者“包含”等类似的词语意指出现该词前面的元件或者物件涵盖出现在该词后面列举的元件或者物件及其等同，而不排除其他元件或者物件。“连接”或者“相连”等类似的词语并非限定于物理的或者机械的连接，而是可以包括电性的连接，不管是直接的还是间接的。Unless otherwise defined, technical terms or scientific terms used in the present invention shall have the usual meaning understood by a person with ordinary skill in the field to which the present invention belongs. "First", "second" and similar words used in the present invention do not indicate any order, quantity or importance, but are only used to distinguish different components. Likewise, similar words such as "a", "an" or "the" do not indicate a quantitative limitation but rather indicate the presence of at least one. Words such as "include" or "comprising" mean that the elements or things appearing before the word include the elements or things listed after the word and their equivalents, without excluding other elements or things. Words such as "connected" or "connected" are not limited to physical or mechanical connections, but may include electrical connections, whether direct or indirect.
需要说明的是，本发明中使用的“上”、“下”、“左”、“右”“前”“后”等仅用于表示相对位置关系，当被描述对象的绝对位置改变后，则该相对位置关系也可能相应地改变。It should be noted that "up", "down", "left", "right", "front", "back", etc. used in the present invention are only used to express relative positional relationships. When the absolute position of the described object changes, Then the relative position relationship may also change accordingly.
结合图1至图6，根据本发明的实施例，提供一种多色荧光检测的检测装置，包括支架1、温控模块2和荧光检测模块3。With reference to Figures 1 to 6, according to an embodiment of the present invention, a detection device for multi-color fluorescence detection is provided, including a bracket 1, a temperature control module 2 and a fluorescence detection module 3.
支架1上固定导轨4、主动轮6和从动轮7，主动轮6和从动轮7之间环绕布置皮带8。皮带8通过皮带压片9将皮带8与荧光检测模块3固定，荧光检测模块3与导轨4滑动安装。The guide rail 4, the driving wheel 6 and the driven wheel 7 are fixed on the bracket 1, and a belt 8 is arranged around the driving wheel 6 and the driven wheel 7. The belt 8 is fixed to the fluorescence detection module 3 through the belt pressing piece 9, and the fluorescence detection module 3 and the guide rail 4 are slidably installed.
如图2所示，温控模块2包括加热板201、温控元件202和散热器203。加热板201置于温控元件202上，温控元件202置于散热器203上。As shown in FIG. 2 , the temperature control module 2 includes a heating plate 201 , a temperature control element 202 and a radiator 203 . The heating plate 201 is placed on the temperature control element 202, and the temperature control element 202 is placed on the radiator 203.
温控模块2包括阵列的多个试管座2011。具体地，多个试管座2011阵列于加热板201上。每一个试管座2011用于放置一个试管10，每一个试管座2011开设一个检测孔2012，多个试管座2011的多个检测孔2012处于同一直线Z，如图1所示。The temperature control module 2 includes a plurality of test tube holders 2011 in an array. Specifically, a plurality of test tube holders 2011 are arrayed on the heating plate 201 . Each test tube holder 2011 is used to place one test tube 10, and each test tube holder 2011 is provided with a detection hole 2012. The multiple detection holes 2012 of multiple test tube holders 2011 are on the same straight line Z, as shown in Figure 1.
如图3所示，荧光检测模块3包括前压盖301、发光壳体302和测光壳体303。发光壳体302内布置发光组件(下文中详细阐述)，测光壳体303内布置测光组件(下文中详细阐述)，前压盖301上开设出光孔3011。As shown in FIG. 3 , the fluorescence detection module 3 includes a front cover 301 , a light-emitting housing 302 and a photometric housing 303 . A light-emitting component (described in detail below) is arranged in the light-emitting housing 302, a photometric component (described in detail below) is arranged in the photometric housing 303, and a light hole 3011 is opened in the front cover 301.
如图1所示，荧光检测模块3布置于温控模块2一侧，荧光检测模块3被配置为：沿多个检测孔2012所处的直线方向(直线Z的方向)往复运动(如图1箭头a所示)，使出光孔3011依次对准每一个试管座2011的检测孔2012。As shown in Figure 1, the fluorescence detection module 3 is arranged on one side of the temperature control module 2, and the fluorescence detection module 3 is configured to reciprocate along the linear direction (the direction of the straight line Z) where the plurality of detection holes 2012 are located (as shown in Figure 1 (shown by arrow a), the light exit hole 3011 is aligned with the detection hole 2012 of each test tube holder 2011 in sequence.
进一步地，支架1固定步进电机5，步进电机5与主动轮6连接，步进电机5驱动主动轮6旋转，主动轮6驱动皮带8运动，皮带8带动荧光检测模块3沿多个检测孔2012所处的直线方向(直线Z的方向)往复运动，使出光孔3011依次对准每一个试管座2011的检测孔2012。Further, the bracket 1 fixes the stepper motor 5, the stepper motor 5 is connected to the driving wheel 6, the stepper motor 5 drives the driving wheel 6 to rotate, the driving wheel 6 drives the belt 8 to move, the belt 8 drives the fluorescence detection module 3 along multiple detection The straight line direction in which the hole 2012 is located (the direction of the straight line Z) reciprocates, so that the light outlet hole 3011 is aligned with the detection hole 2012 of each test tube holder 2011 in sequence.
结合图3、图4和图5，根据本发明的实施例，发光壳体302内布置发光组件，发光组件包括多色发光元件304、发光电路板305、发光透镜306和发光多通滤光片307。3, 4 and 5, according to an embodiment of the present invention, a light-emitting component is arranged in the light-emitting housing 302. The light-emitting component includes a multi-color light-emitting element 304, a light-emitting circuit board 305, a light-emitting lens 306 and a light-emitting multi-pass filter. 307.
发光电路板305与发光壳体302固定，多色发光元件304与发光电路板305连接，发光透镜306置于多色发光元件304和发光多通滤光片307之间。发光透镜306，用于将多色发光元件304发出的不同波谱范围的激发光传输至发光多通滤光片307。The light-emitting circuit board 305 is fixed to the light-emitting housing 302, the multi-color light-emitting element 304 is connected to the light-emitting circuit board 305, and the light-emitting lens 306 is placed between the multi-color light-emitting element 304 and the light-emitting multi-pass filter 307. The light-emitting lens 306 is used to transmit the excitation light in different spectral ranges emitted by the multi-color light-emitting element 304 to the light-emitting multi-pass filter 307 .
测光壳体303内布置测光组件，测光组件包括测光多通滤光片311、测光透镜310、测光元件308和测光电路板309。A photometric assembly is arranged inside the photometric housing 303 . The photometric assembly includes a photometric multi-pass filter 311 , a photometric lens 310 , a photometric element 308 and a photometric circuit board 309 .
测光电路板309与测光壳体303固定，测光元件308与测光电路板309连接，测光透镜310置于测光多通滤光片311和测光元件308之间。测光透镜310，用于将测光多通滤光片311分离的不同波长的荧光传输至测光元件308。The photometric circuit board 309 is fixed to the photometric housing 303 , the photometric element 308 is connected to the photometric circuit board 309 , and the photometric lens 310 is placed between the photometric multi-pass filter 311 and the photometric element 308 . The photometric lens 310 is used to transmit the fluorescence of different wavelengths separated by the photometric multi-pass filter 311 to the photometric element 308 .
根据本发明的实施例，在发光多通滤光片307和测光多通滤光片311之间倾斜45度角布置一分色镜312。倾斜45度角布置的分色镜312等效于45°入射的滤光片，其通带内的光可以直接透射，止带的光被反射，沿与入射光垂直方向出射。According to an embodiment of the present invention, a dichroic mirror 312 is arranged at an angle of 45 degrees between the luminescent multi-pass filter 307 and the photometric multi-pass filter 311 . The dichroic mirror 312 arranged at an angle of 45 degrees is equivalent to a 45-degree incident filter. The light in its passband can be directly transmitted, and the light in the stopband is reflected and emitted in a direction perpendicular to the incident light.
前压盖301上开设出光孔3011，前压盖301内安装出光透镜313，出光透313镜正对出光孔3011。A light-emitting hole 3011 is opened in the front pressure cover 301. A light-emitting lens 313 is installed in the front pressure cover 301. The light-emitting lens 313 faces the light-emitting hole 3011.
如图5所示，根据本发明的实施例，发光组件和测光组件的光轴垂直，并且发光多通滤光片307与出光孔3011位于分色镜312的同一侧，并且发光组件的光轴与出光孔3011的轴线处于同一直线。As shown in Figure 5, according to the embodiment of the present invention, the optical axes of the light-emitting component and the photometric component are perpendicular, and the light-emitting multi-pass filter 307 and the light exit hole 3011 are located on the same side of the dichroic mirror 312, and the light of the light-emitting component The axis is in the same straight line as the axis of the light outlet 3011.
即多色发光元件304、发光透镜306和发光多通滤光片307的光轴，与光多通滤光片311、测光透镜310和测光元件308的光轴垂直，并且光多通滤光片311、测光透镜310和测光元件308的光轴与出光透镜313、出光孔3011的轴线重合。That is, the optical axes of the multi-color light-emitting element 304, the light-emitting lens 306 and the light-emitting multi-pass filter 307 are perpendicular to the optical axes of the light multi-pass filter 311, the photometric lens 310 and the photometric element 308, and the light multi-pass filter The optical axes of the light sheet 311, the photometric lens 310 and the photometric element 308 coincide with the axes of the light exit lens 313 and the light exit hole 3011.
根据本发明的实施例，多色发光元件304的波谱范围数与发光多通滤光片307的通带数相等；多色发光元件304的波谱范围数与测光多通滤光片311的通带数相等；测光多通滤光片3011的通带数与分色镜312的通带数相等。试管10内置多种不同染料和被测靶标，每种染料的荧光检测强度与被测靶标的浓度正相关。According to an embodiment of the present invention, the number of spectral ranges of the multicolor light-emitting element 304 is equal to the number of passbands of the luminescent multipass filter 307; The band numbers are equal; the pass band number of the photometric multi-pass filter 3011 is equal to the pass band number of the dichroic mirror 312. The test tube 10 contains a variety of different dyes and targets to be measured, and the fluorescence detection intensity of each dye is positively related to the concentration of the target to be measured.
图6所示，本实施例中示例的试管10内置三种不同染料和被测靶标(样本)，每种染料的荧光检测强度与被测靶标(样本)的浓度正相关。As shown in FIG. 6 , the test tube 10 in this embodiment contains three different dyes and the measured target (sample). The fluorescence detection intensity of each dye is positively related to the concentration of the measured target (sample).
多色发光元件304发出三种不同波谱范围的激发光，即多色发光元件304发出三种不同波谱范围的激发光分别为：第一波谱范围P1的激发光、第二波谱范围P2的激发光和第三波谱范围P3的激发光。The multi-color light-emitting element 304 emits excitation light in three different spectrum ranges. That is, the multi-color light-emitting element 304 emits excitation light in three different spectrum ranges: excitation light in the first spectrum range P1 and excitation light in the second spectrum range P2. and excitation light in the third spectral range P3.
发光多通滤光片307具有三个通带，即发光多通滤光片307具有的三个通带分别为：发光多通滤光片第一通带F1、发光多通滤光片第二通带F2和发光多通滤光片第三通带F3。发光多通滤光片307的三个通带将三种不同波谱范围的激发光分离出三种波长的激发单色光。The luminescent multipass filter 307 has three passbands, that is, the three passbands of the luminescent multipass filter 307 are: the first passband F1 of the luminescent multipass filter, and the second passband of the luminescent multipass filter 307 . Passband F2 and the third passband F3 of the luminescent multipass filter. The three passbands of the luminescent multi-pass filter 307 separate the excitation light in three different spectral ranges into three wavelengths of excitation monochromatic light.
测光多通滤光片311具有三个通带，即测光多通滤光片311具有的三个通带分别为：测光多通滤光片第一通带C1、测光多通滤光片第二通带C2和测光多通滤光片第三通带C3。测光多通滤光片311的三个通带将试管10内的三种荧光染料产生三种波长的荧光分离。The photometric multipass filter 311 has three passbands, that is, the three passbands of the photometric multipass filter 311 are: the first passband C1 of the photometric multipass filter, the first passband C1 of the photometric multipass filter, and the first passband C1 of the photometric multipass filter. The second passband C2 of the light sheet and the third passband C3 of the photometric multipass filter. The three passbands of the photometric multi-pass filter 311 separate the three wavelengths of fluorescence produced by the three fluorescent dyes in the test tube 10 .
分色镜312具有三个通带，即分色镜312具有的三个通带分别为：分色镜第一通带D1、分色镜第二通带D2和分色镜第三通带D3。The dichroic mirror 312 has three passbands, that is, the three passbands of the dichroic mirror 312 are respectively: the first passband D1 of the dichroic mirror, the second passband D2 of the dichroic mirror, and the third passband D3 of the dichroic mirror. .
根据本发明的实施例，多色发光元件304的一个波谱范围对应发光多通滤光片307的一个通带。According to an embodiment of the present invention, a spectrum range of the multi-color light-emitting element 304 corresponds to a pass band of the light-emitting multi-pass filter 307 .
本实施例中，多色发光元件304的第一波谱范围P1对应发光多通滤光片307的发光多通滤光片第一通带F1，并且多色发光元件304的第一波谱范围P1与发光多通滤光片307的发光多通滤光片第二通带F2不重叠，多色发光元件304的第一波谱范围P1与发光多通滤光片307的发光多通滤光片第三通带F3不重叠。In this embodiment, the first spectral range P1 of the multi-color light-emitting element 304 corresponds to the first passband F1 of the light-emitting multi-pass filter 307, and the first spectral range P1 of the multi-color light-emitting element 304 is consistent with The second passband F2 of the luminescent multipass filter 307 does not overlap, and the first spectral range P1 of the multicolor light-emitting element 304 is the third passband of the luminescent multipass filter 307 . Passband F3 does not overlap.
同样地，多色发光元件304的第二波谱范围P2对应发光多通滤光片307的发光多通滤光片第二通带F2，并且多色发光元件304的第二波谱范围P2与发光多通滤光片307的发光多通滤光片第一通带F1不重叠，多色发光元件304的第二波谱范围P2与发光多通滤光片307的发光多通滤光片第三通带F3不重叠。Similarly, the second spectrum range P2 of the multi-color light-emitting element 304 corresponds to the second passband F2 of the light-emitting multi-pass filter 307, and the second spectrum range P2 of the multi-color light-emitting element 304 is consistent with the light-emitting multi-pass filter 307. The first passband F1 of the light-emitting multi-pass filter 307 does not overlap, and the second spectral range P2 of the multi-color light-emitting element 304 is the same as the third pass-band of the light-emitting multi-pass filter 307 F3 does not overlap.
同样地，多色发光元件304的第三波谱范围P3对应发光多通滤光片307的发光多通滤光片第三通带F3，并且多色发光元件304的第三波谱范围P3与发光多通滤光片307的发光多通滤光片第一通带F1不重叠，多色发光元件304的第三波谱范围P3与发光多通滤光片307的发光多通滤光片第二通带F3不重叠。Similarly, the third spectrum range P3 of the multi-color light-emitting element 304 corresponds to the third pass band F3 of the light-emitting multi-pass filter 307, and the third spectrum range P3 of the multi-color light-emitting element 304 is consistent with the light-emitting multi-pass filter 307. The first passband F1 of the light-emitting multi-pass filter 307 does not overlap, and the third spectrum range P3 of the multi-color light-emitting element 304 and the second pass-band of the light-emitting multi-pass filter 307 F3 does not overlap.
根据本发明的实施例，发光多通滤光片307的每一个通带与测光多通滤光片311的每一个通带彼此不重叠。According to an embodiment of the present invention, each passband of the luminescent multipass filter 307 and each passband of the photometric multipass filter 311 do not overlap with each other.
本实施例中，发光多通滤光片307的发光多通滤光片第一通带F1与测光多通滤光片311的测光多通滤光片第一通带C1不重叠；发光多通滤光片307的发光多通滤光片第一通带F1与测光多通滤光片311的测光多通滤光片第二通带C2不重叠；发光多通滤光片307的发光多通滤光片第一通带F1与测光多通滤光片311的测光多通滤光片第三通带C3不重叠。In this embodiment, the first passband F1 of the luminescent multipass filter 307 does not overlap with the first passband C1 of the photometric multipass filter 311 of the photometric multipass filter; luminescence The first passband F1 of the luminescent multipass filter of the multipass filter 307 does not overlap with the second passband C2 of the photometric multipass filter of the photometric multipass filter 311; the luminescent multipass filter 307 The first passband F1 of the luminescence multipass filter does not overlap with the third passband C3 of the photometric multipass filter 311 .
并且，发光多通滤光片307的发光多通滤光片第二通带F2与测光多通滤光片311的测光多通滤光片第一通带C1不重叠；发光多通滤光片307的发光多通滤光片第二通带F2与测光多通滤光片311的测光多通滤光片第二通带C2不重叠；发光多通滤光片307的发光多通滤光片第二通带F2与测光多通滤光片311的测光多通滤光片第三通带C3不重叠。Moreover, the second passband F2 of the luminescent multipass filter 307 does not overlap with the first passband C1 of the photometric multipass filter 311 of the photometric multipass filter; the luminescent multipass filter The second passband F2 of the luminescent multipass filter of the light sheet 307 does not overlap with the second passband C2 of the photometric multipass filter 311 of the photometric multipass filter; The second passband F2 of the pass filter does not overlap with the third passband C3 of the photometric multipass filter 311 .
并且，发光多通滤光片307的发光多通滤光片第三通带F3与测光多通滤光片311的测光多通滤光片第一通带C1不重叠；发光多通滤光片307的发光多通滤光片第三通带F3与测光多通滤光片311的测光多通滤光片第二通带C2不重叠；发光多通滤光片307的发光多通滤光片第三通带F3与测光多通滤光片311的测光多通滤光片第三通带C3不重叠。Moreover, the third passband F3 of the luminescent multipass filter 307 does not overlap with the first passband C1 of the photometric multipass filter 311 of the photometric multipass filter; the luminescent multipass filter The third passband F3 of the luminescent multipass filter of the light sheet 307 does not overlap with the second passband C2 of the photometric multipass filter 311 of the photometric multipass filter; The third passband F3 of the pass filter does not overlap with the third passband C3 of the photometric multipass filter 311 .
根据本发明的实施例，发光多通滤光片307的所有通带之间彼此不重叠，测光多通滤光片311的所有通带之间彼此不重叠。According to an embodiment of the present invention, all passbands of the luminescent multipass filter 307 do not overlap with each other, and all passbands of the photometric multipass filter 311 do not overlap with each other.
本实施例中，发光多通滤光片307的发光多通滤光片第一通带F1、发光多通滤光片第二通带F2和发光多通滤光片第三通带F3之间彼此不重叠，并且测光多通滤光片311的测光多通滤光片第一通带C1、测光多通滤光片第二通带C2和测光多通滤光片第三通带C3之间彼此不重叠。In this embodiment, between the first passband F1 of the luminescent multipass filter 307 , the second passband F2 of the luminescent multipass filter and the third passband F3 of the luminescent multipass filter The first passband C1 of the photometric multipass filter, the second passband C2 of the photometric multipass filter, and the third passband of the photometric multipass filter 311 do not overlap each other. Bands C3 do not overlap each other.
根据本发明的实施例，分色镜312的一个通带对应测光多通滤光片311的一个通带，并且发光多通滤光片307的每一个通带与分色镜312的每一个通带彼此不重叠。According to an embodiment of the present invention, a passband of the dichroic mirror 312 corresponds to a passband of the photometric multipass filter 311 , and each passband of the luminescent multipass filter 307 corresponds to each passband of the dichroic mirror 312 The passbands do not overlap each other.
本实施例中，分色镜312的分色镜第一通带D1对应测光多通滤光片311的测光多通滤光片第一通带C1，即色镜312的分色镜第一通带D1包含测光多通滤光片311的测光多通滤光片第一通带C1。In this embodiment, the first passband D1 of the dichroic mirror 312 corresponds to the first passband C1 of the photometric multipass filter 311 , that is, the first passband D1 of the dichroic mirror 312 A passband D1 includes a first passband C1 of the photometric multipass filter 311 .
分色镜312的分色镜第二通带D2对应测光多通滤光片311的测光多通滤光片第二通带C2，即分色镜312的分色镜第二通带D2包含测光多通滤光片311的测光多通滤光片第二通带C2。The second passband D2 of the dichroic mirror 312 corresponds to the second passband C2 of the photometric multipass filter 311 , that is, the second passband D2 of the dichroic mirror 312 The photometric multi-pass filter includes a second passband C2 of the photometric multi-pass filter 311 .
分色镜312的分色镜第三通带D3对应测光多通滤光片311的测光多通滤光片第三通带C3，即分色镜312的分色镜第三通带D3包含测光多通滤光片311的测光多通滤光片第三通带C3。The third passband D3 of the dichroic mirror 312 corresponds to the third passband C3 of the photometric multipass filter 311 , that is, the third passband D3 of the dichroic mirror 312 The photometric multi-pass filter includes a third passband C3 of the photometric multi-pass filter 311 .
并且，发光多通滤光片307的发光多通滤光片第一通带F1与分色镜312的分色镜第一通带D1不重叠；发光多通滤光片307的发光多通滤光片第一通带F1与分色镜312的分色镜第二通带D2不重叠；发光多通滤光片307的发光多通滤光片第一通带F1与分色镜312的分色镜第三通带D3不重叠。Moreover, the first passband F1 of the luminescent multipass filter 307 does not overlap with the first passband D1 of the dichroic mirror 312; The first passband F1 of the light sheet does not overlap with the second passband D2 of the dichroic mirror 312; The third passband D3 of the color mirror does not overlap.
并且，发光多通滤光片307的发光多通滤光片第二通带F2与分色镜312的分色镜第一通带D1不重叠；发光多通滤光片307的发光多通滤光片第二通带F2与分色镜312的分色镜第二通带D2不重叠；发光多通滤光片307的发光多通滤光片第二通带F2与分色镜312的分色镜第三通带D3不重叠。Moreover, the second passband F2 of the luminescent multipass filter 307 does not overlap with the first passband D1 of the dichroic mirror 312; The second passband F2 of the light sheet does not overlap with the second passband D2 of the dichroic mirror 312; The third passband D3 of the color mirror does not overlap.
并且，发光多通滤光片307的发光多通滤光片第三通带F3与分色镜312的分色镜第一通带D1不重叠；发光多通滤光片307的发光多通滤光片第三通带F3与分色镜312的分色镜第二通带D2不重叠；发光多通滤光片307的发光多通滤光片第三通带F3与分色镜312的分色镜第三通带D3不重叠。Moreover, the third passband F3 of the luminescent multipass filter 307 does not overlap with the first passband D1 of the dichroic mirror 312; The third passband F3 of the light sheet does not overlap with the second passband D2 of the dichroic mirror 312; The third passband D3 of the color mirror does not overlap.
根据本发明的实施例，分色镜312的所有通带之间彼此不重叠。According to an embodiment of the present invention, all passbands of the dichroic mirror 312 do not overlap with each other.
本实施例中，分色镜312的分色镜第一通带D1、分色镜312的分色镜第二通带D2和分色镜312的分色镜第三通带D3之间彼此不重叠。In this embodiment, the first passband D1 of the dichroic mirror 312 , the second passband D2 of the dichroic mirror 312 and the third passband D3 of the dichroic mirror 312 are different from each other. overlapping.
根据本发明的实施例，发光多通滤光片307的一个通带对应一种染料的激发波长；测光多通滤光片311的一个通带对应一种染料的荧光波长。According to an embodiment of the present invention, one passband of the luminescent multipass filter 307 corresponds to the excitation wavelength of a dye; one passband of the photometric multipass filter 311 corresponds to the fluorescence wavelength of a dye.
本实施例中，三种不同染料分别为第一种染料、第二种染料和第三种染料。第一种染料的第一种染料激发波长为J1，第二种染料的第二种染料激发波长为J2，第三种染料的第三种染料激发波长为J3。In this embodiment, the three different dyes are the first dye, the second dye and the third dye respectively. The first dye excitation wavelength of the first dye is J1, the second dye excitation wavelength of the second dye is J2, and the third dye excitation wavelength of the third dye is J3.
第一种染料的第一种染料荧光波长为Y1，第二种染料的第二种染料荧光波长为Y2，第三种染料的第三种染料荧光波长为Y3。The fluorescence wavelength of the first dye of the first dye is Y1, the fluorescence wavelength of the second dye of the second dye is Y2, and the fluorescence wavelength of the third dye of the third dye is Y3.
发光多通滤光片307的发光多通滤光片第一通带F1对应第一种染料的第一种染料激发波长J1，即发光多通滤光片307的发光多通滤光片第一通带F1包含第一种染料的第一种染料激发波长J1。The first passband F1 of the luminescent multipass filter 307 corresponds to the first dye excitation wavelength J1 of the first dye, that is, the first passband F1 of the luminescent multipass filter 307 Passband F1 contains the first dye excitation wavelength J1 of the first dye.
发光多通滤光片307的发光多通滤光片第二通带F2对应第二种染料的第二种染料激发波长J2，即发光多通滤光片307的发光多通滤光片第二通带F2包含第二种染料的第二种染料激发波长J2。The second passband F2 of the luminescent multipass filter 307 corresponds to the second dye excitation wavelength J2 of the second dye, that is, the second passband F2 of the luminescent multipass filter 307 Passband F2 contains the second dye excitation wavelength J2 of the second dye.
发光多通滤光片307的发光多通滤光片第三通带F3对应第三种染料的第三种染料激发波长J3，即发光多通滤光片307的发光多通滤光片第三通带F3包含第三种染料的第三种染料激发波长J3。The third passband F3 of the luminescent multipass filter 307 corresponds to the third dye excitation wavelength J3 of the third dye, that is, the third passband F3 of the luminescent multipass filter 307 Passband F3 contains the third dye excitation wavelength J3 of the third dye.
测光多通滤光片311的测光多通滤光片第一通带C1对应第一种染料的第一种染料荧光波长Y1，即测光多通滤光片311的测光多通滤光片第一通带C1包含第一种染料的第一种染料荧光波长Y1。The first passband C1 of the photometric multipass filter 311 corresponds to the first dye fluorescence wavelength Y1 of the first dye, that is, the photometric multipass filter 311 The first passband C1 of the light sheet includes the first dye fluorescence wavelength Y1 of the first dye.
测光多通滤光片311的测光多通滤光片第二通带C2对应第二种染料的第二种染料荧光波长Y2，即测光多通滤光片311测光多通滤光片第二通带C21包含第二种染料的第二种染料荧光波长Y2。The second passband C2 of the photometric multipass filter 311 corresponds to the second dye fluorescence wavelength Y2 of the second dye, that is, the photometric multipass filter 311 photometric multipass filter The second passband C21 of the slice contains the second dye fluorescence wavelength Y2 of the second dye.
测光多通滤光片311的测光多通滤光片第三通带C3对应第三种染料的第三种染料荧光波长Y3，即测光多通滤光片311的测光多通滤光片第三通带C3包含第三种染料的第三种染料荧光波长Y3。The third passband C3 of the photometric multipass filter 311 corresponds to the third dye fluorescence wavelength Y3 of the third dye, that is, the photometric multipass filter 311 The third passband C3 of the light sheet includes the third dye fluorescence wavelength Y3 of the third dye.
根据本发明的实施例，提供一种多色荧光检测的检测方法，所述检测方法包括如下方法步骤：According to an embodiment of the present invention, a detection method for multi-color fluorescence detection is provided. The detection method includes the following method steps:
多色发光元件304发出不同波谱范围的激发光。The multicolor light-emitting element 304 emits excitation light in different spectral ranges.
根据本发明的实施例，多色发光元件304的波谱范围数与发光多通滤光片307的通带数相等，多色发光元件304的波谱范围数与测光多通滤光片311的通带数相等；测光多通滤光片311的通带数与分色镜312的通带数相等。According to the embodiment of the present invention, the number of spectral ranges of the multi-color light-emitting element 304 is equal to the number of pass bands of the luminescence multi-pass filter 307, and the number of spectral ranges of the multi-color light-emitting element 304 is equal to the number of pass bands of the photometric multi-pass filter 311. The band numbers are equal; the pass band number of the photometric multi-pass filter 311 is equal to the pass band number of the dichroic mirror 312 .
多色发光元件304的一个波谱范围对应发光多通滤光片307的一个通带。发光多通滤光片307的每一个通带与测光多通滤光片311的每一个通带彼此不重叠。A spectral range of the multi-color light-emitting element 304 corresponds to a pass band of the light-emitting multi-pass filter 307 . Each passband of the luminescence multipass filter 307 and each passband of the photometric multipass filter 311 do not overlap with each other.
分色镜312的一个通带对应测光多通滤光片311的一个通带，并且发光多通滤光片307的每一个通带与分色镜312的每一个通带彼此不重叠；A passband of the dichroic mirror 312 corresponds to a passband of the photometric multipass filter 311, and each passband of the luminescent multipass filter 307 does not overlap with each passband of the dichroic mirror 312;
发光多通滤光片307的所有通带之间彼此不重叠，测光多通滤光片311的所有通带之间彼此不重叠，分色镜312的所有通带之间彼此不重叠。All passbands of the luminescence multipass filter 307 do not overlap with each other, all passbands of the photometric multipass filter 311 do not overlap with each other, and all passbands of the dichroic mirror 312 do not overlap with each other.
发光多通滤光片307的一个通带对应一种染料的激发波长；测光多通滤光片311的一个通带对应一种染料的荧光波长。One passband of the luminescent multipass filter 307 corresponds to the excitation wavelength of a dye; one passband of the photometric multipass filter 311 corresponds to the fluorescence wavelength of a dye.
本实施例中，以试管10内置三种不同染料和被测靶标(样本)，多色发光元件304发出三种不同波谱范围的激发光为例。In this embodiment, it is taken as an example that the test tube 10 contains three different dyes and a target (sample) to be measured, and the multicolor light-emitting element 304 emits excitation light in three different spectrum ranges.
发光电路板305控制多色发光元件402发出的三种波谱范围(第一波谱范围P1、第二波谱范围P2和第三波谱范围P3)的激发光，并经发光透镜306传输至发光多通滤光片307。The light-emitting circuit board 305 controls the excitation light in three spectrum ranges (the first spectrum range P1, the second spectrum range P2 and the third spectrum range P3) emitted by the multi-color light-emitting element 402, and transmits it to the luminescence multi-pass filter through the luminescence lens 306. Light sheet 307.
发光多通滤光片307，将多色发光元件304的不同波谱范围的激发光，分离出不同波长的激发单色光。The light-emitting multi-pass filter 307 separates the excitation light in different spectral ranges of the multi-color light-emitting element 304 into excitation monochromatic light of different wavelengths.
本实施例中，发光透镜306将多色发光元件304发出的三种波谱范围(第一波谱范围P1、第二波谱范围P2和第三波谱范围P3)的激发光传输至发光多通滤光片307。In this embodiment, the luminescent lens 306 transmits the excitation light in three spectrum ranges (the first spectrum range P1, the second spectrum range P2, and the third spectrum range P3) emitted by the multicolor light-emitting element 304 to the luminescence multi-pass filter. 307.
由于发光多通滤光片307的一个通带对应一种染料的激发波长，发光多通滤光片307的发光多通滤光片第一通带F1将多色发光元件304的第一波谱范围P1的激发光分离出第一种染料激发波长J1的激发单色光；Since one passband of the luminescent multi-pass filter 307 corresponds to the excitation wavelength of a dye, the first passband F1 of the luminescent multi-pass filter 307 covers the first spectral range of the multi-color light-emitting element 304 The excitation light of P1 separates the excitation monochromatic light of the first dye excitation wavelength J1;
发光多通滤光片307的发光多通滤光片第二通带F2将多色发光元件304的第二波谱范围P2的激发光分离出第二种染料激发波长J2的激发单色光；The second passband F2 of the luminescent multipass filter 307 separates the excitation light of the second spectral range P2 of the multicolor light-emitting element 304 into the excitation monochromatic light of the second dye excitation wavelength J2;
发光多通滤光片307的发光多通滤光片第三通带F3将多色发光元件304的第三波谱范围P3的激发光分离出第三种染料激发波长J3的激发单色光。The third passband F3 of the luminescent multipass filter 307 separates the excitation light of the third spectral range P3 of the multicolor light-emitting element 304 into the excitation monochromatic light of the third dye excitation wavelength J3.
分色镜312，将不同波长的激发单色光垂直反射至出光孔3011，并经试管座2011的检测孔2012射入试管10内，激发试管10内的多种不同染料产生不同波长的荧光。The dichroic mirror 312 vertically reflects the excitation monochromatic light of different wavelengths to the light outlet hole 3011, and injects it into the test tube 10 through the detection hole 2012 of the test tube holder 2011, thereby exciting a variety of different dyes in the test tube 10 to produce fluorescence of different wavelengths.
由于发光多通滤光片307的每一个通带与分色镜312的每一个通带彼此不重叠，本实施例中，分色镜312将三种波长的激发单色光(第一种染料激发波长J1的激发单色光、第二种染料激发波长J2的激发单色光和第三种染料激发波长J3的激发单色光)垂直反射至出光透镜313，并经出光孔3011、试管座2011的检测孔2012射入试管10内，激发试管10内的三种不同染料产生三种不同波长的荧光。Since each passband of the luminescent multipass filter 307 and each passband of the dichroic mirror 312 do not overlap with each other, in this embodiment, the dichroic mirror 312 excites the monochromatic light of three wavelengths (the first dye The excitation monochromatic light of the excitation wavelength J1, the excitation monochromatic light of the second dye excitation wavelength J2, and the excitation monochromatic light of the third dye excitation wavelength J3) are vertically reflected to the light exit lens 313, and pass through the light exit hole 3011 and the test tube holder. The detection hole 2012 of 2011 is injected into the test tube 10, and three different dyes in the test tube 10 are excited to produce fluorescence of three different wavelengths.
即，三种染料分别产生第一种染料的第一种染料荧光波长为Y1，第二种染料的第二种染料荧光波长为Y2，第三种染料的第三种染料荧光波长为Y3。That is, the three dyes respectively produce the first dye whose fluorescence wavelength is Y1, the second dye whose fluorescence wavelength is Y2, and the third dye whose fluorescence wavelength is Y3.
不同波长的荧光反射至分色镜312，分色镜312将不同波长的荧光传输至测光多通滤光片311。Fluorescence of different wavelengths is reflected to the dichroic mirror 312 , and the dichroic mirror 312 transmits the fluorescence of different wavelengths to the photometric multi-pass filter 311 .
本实施例中，三种不同波长的荧光(第一种染料的第一种染料荧光波长Y1，第二种染料的第二种染料荧光波长Y2，第三种染料的第三种染料荧光波长Y3)经试管座2011的检测孔2012、出光孔3011和出光透镜313反射至分色镜312。由于分色镜312的一个通带对应测光多通滤光片311的一个通带，测光多通滤光片311的一个通带对应一种染料的荧光波长，三种不同波长的荧光(第一种染料的第一种染料荧光波长Y1的荧光，第二种染料的第二种染料荧光波长Y2的荧光，第三种染料的第三种染料荧光波长Y3的荧光)穿过分色镜312传输至测光多通滤光片311。In this embodiment, three different wavelengths of fluorescence (the first dye fluorescence wavelength Y1 of the first dye, the second dye fluorescence wavelength Y2 of the second dye, and the third dye fluorescence wavelength Y3 of the third dye ) is reflected to the dichroic mirror 312 through the detection hole 2012, the light exit hole 3011 and the light exit lens 313 of the test tube holder 2011. Since a passband of the dichroic mirror 312 corresponds to a passband of the photometric multipass filter 311, and a passband of the photometric multipass filter 311 corresponds to the fluorescence wavelength of one dye, the fluorescence of three different wavelengths ( The fluorescence of the first dye of the first dye fluorescence wavelength Y1, the fluorescence of the second dye of the second dye fluorescence wavelength Y2, the fluorescence of the third dye of the third dye fluorescence wavelength Y3) pass through the dichroic mirror 312 Transmitted to the photometric multi-pass filter 311.
测光多通滤光片311，分离不同波长的荧光，并将不同波长的荧光传输至测光元件。The photometric multi-pass filter 311 separates the fluorescence of different wavelengths and transmits the fluorescence of different wavelengths to the photometric element.
本实施例中，测光多通滤光片311，分离三种波长的荧光(第一种染料荧光波长Y1的荧光、第二种染料荧光波长Y2的荧光和第三种染料荧光波长Y3的荧光)，并将三种波长的荧光(第一种染料荧光波长Y1的荧光、第二种染料荧光波长Y2的荧光和第三种染料荧光波长Y3的荧光)经测光透镜310传输至测光元件308，测光元件308将三种波长的荧光转换为电信号传输至测光电路板309进行多色荧光检测。In this embodiment, the photometric multi-pass filter 311 separates the fluorescence of three wavelengths (the fluorescence of the first dye fluorescence wavelength Y1, the fluorescence of the second dye fluorescence wavelength Y2, and the fluorescence of the third dye fluorescence wavelength Y3 ), and transmit the fluorescence of three wavelengths (the fluorescence of the first dye fluorescence wavelength Y1, the fluorescence of the second dye fluorescence wavelength Y2, and the fluorescence of the third dye fluorescence wavelength Y3) to the photometric element through the photometric lens 310 308. The photometric element 308 converts the fluorescence of three wavelengths into electrical signals and transmits them to the photometric circuit board 309 for multi-color fluorescence detection.
有以下几点需要说明：The following points need to be explained:
(1)本发明实施例附图只涉及到与本发明实施例涉及到的结构，其他结构可参考通常设计。(1) The drawings of the embodiments of the present invention only refer to the structures involved in the embodiments of the present invention, and other structures can refer to the general design.
(2)为了清晰起见，在用于描述本发明的实施例的附图中，层或区域的厚度被放大或缩小，即这些附图并非按照实际的比例绘制。可以理解，当诸如层、膜、区域或基板之类的元件被称作位于另一元件“上”或“下”时，该元件可以“直接”位于另一元件“上”或“下”或者可以存在中间元件。(2) For the sake of clarity, in the drawings used to describe embodiments of the present invention, the thicknesses of layers or regions are exaggerated or reduced, that is, these drawings are not drawn according to actual scale. It will be understood that when an element such as a layer, film, region or substrate is referred to as being "on" or "under" another element, it can be "directly on" or "under" the other element or Intermediate elements may be present.
(3)在不冲突的情况下，本发明的实施例及实施例中的特征可以相互组合以得到新的实施例。(3) Without conflict, the embodiments of the present invention and the features in the embodiments can be combined with each other to obtain new embodiments.
以上，仅为本发明的具体实施方式，但本发明的保护范围并不局限于此，本发明的保护范围应以权利要求的保护范围为准。The above are only specific embodiments of the present invention, but the protection scope of the present invention is not limited thereto. The protection scope of the present invention should be subject to the protection scope of the claims.
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