CN211554382U - Multi-wavelength light path selector - Google Patents
Multi-wavelength light path selector Download PDFInfo
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- CN211554382U CN211554382U CN201922001234.9U CN201922001234U CN211554382U CN 211554382 U CN211554382 U CN 211554382U CN 201922001234 U CN201922001234 U CN 201922001234U CN 211554382 U CN211554382 U CN 211554382U
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Abstract
The utility model discloses a multi-wavelength light path selector, multi-wavelength light path selector include the different light path passageway of a plurality of wavelengths, and a plurality of light path passageways correspond a plurality of dichroic mirrors respectively, and a plurality of lights of penetrating into in the light path passageway are passed through and are jetted out from an exit port behind dichroic mirror reflection or transmission. The utility model has the advantages of, use an optic fibre can send into reaction unit, reduce cost, easy to use with the exciting light that just can will be with different light paths. And the light path structure is simple, the volume is small, and the manufacture is easy.
Description
Technical Field
The utility model relates to an optical path selector, concretely relates to optical path selector of multi-wavelength.
Background
The real-time fluorescence PCR technology can realize the quantitative analysis of the DNA template and has important significance for molecular biology research, medical research and the like. The fluorescence device of the real-time fluorescence quantitative PCR instrument is the core of the real-time fluorescence quantitative PCR instrument, the traditional fluorescence detection device has a huge mechanical structure and a complex light path, and only a few powerful major companies, major enterprises and national key laboratories are purchased, so that the development of scientific research and practical application in the field is influenced. The core of the fluorescence device is that excitation light with different wavelengths is sent to the reaction device through different light paths, emission light with longer wavelengths is excited, and the emission light is sent to the photoelectric detector with corresponding wavelengths through different light paths. Like the real-time fluorescence quantitative PCR detecting system of rotary scanning that patent number CN201410811875X discloses, prior art when detecting a plurality of substances that await measuring, needs to use many optic fibre to connect light source excitation device and thermal cycle module, and the optic fibre is connected complicatedly when the substance that awaits measuring is more, and need confirm the optic fibre at the hookup location of light source excitation device and thermal cycle module according to the kind of the substance that awaits measuring, and the structure is complicated, difficult use.
Therefore, there is a high necessity for a light path selector having a simple structure, which can feed excitation lights of different light paths into a reaction device using one optical fiber.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a simple structure uses an optic fibre just can send into the exciting light of different light paths into reaction unit's light path selector
The PCR technique is called polymerase chain reaction, and is a molecular biological technique for amplifying a large amount of a specific DNA fragment in a short time. Under the catalysis of DNA polymerase, taking the mother strand DNA as a template and a specific primer as an extension starting point, and carrying out the steps of denaturation, annealing, extension and the like to copy daughter strand DNA complementary to the mother strand template DNA in vitro. Fluorescent PCR technology is based on the PCR technique of adding fluorescent dyes or fluorescently labeled specific probes to the PCR reaction, each fluorescently labeled specific probe having a respective excitation wavelength, and irradiation with excitation light of a specific wavelength can produce emission light of longer wavelength. And the intensity of the emitted light is in positive correlation with the quantity of the PCR reaction products, and the quantity of the PCR reaction products can be monitored by monitoring the intensity of the emitted light. The PCR reaction can be added with a plurality of specific probes which are fluorescently labeled, exciting light with corresponding wavelength is used for irradiating the PCR reaction, and the intensity of emitted light is monitored by a photoelectric detector, so that the amount of corresponding PCR reaction products can be checked.
Dichroic mirrors are divided into high-pass dichroic mirrors and short-pass dichroic mirrors, and have different cut-off wavelengths due to differences in respective optical properties. When light is incident on the dichroic mirror at an angle of 45 degrees, the high-pass dichroic mirror has high reflectivity for light beams below a cut-off wavelength and high transmittance for light beams above the cut-off wavelength; the short-wave pass dichroic mirror has high reflectivity for light beams above a cut-off wavelength and high transmittance for light beams below the cut-off wavelength.
Filters allow light of a specific wavelength range to pass through, and we use narrow band filters to obtain the desired, specific wavelength of light.
If there are many kinds of substances to be tested in the fluorescence PCR technology, then multiple excitation lights with different wavelengths are needed to irradiate the substances to be tested, and a light path selector capable of passing through the excitation lights with multiple specific wavelengths is needed. The light with various specific wavelengths can be generated by using various light emitting diodes, light beams generated by the light emitting diodes are emitted into a narrow-band filter through a light path pipeline to filter exciting light with the specific wavelengths, the exciting light with the specific wavelengths is emitted into a dichroic mirror through a light path channel, and the dichroic mirror can reflect light beams with the wavelengths lower than the cut-off wavelength and pass light beams with the wavelengths higher than the cut-off wavelength; or to reflect light beams above the cut-off wavelength and pass light beams below the cut-off wavelength. The light beam entering the dichroic mirror may be reflected, or the dichroic mirror may be combined in various ways, and the light beam may be transmitted to the light path selector exit port. The exit port of the light path selector is connected with an optical fiber, and the optical fiber sends exciting light into the PCR reactor.
The optical path channel refers to a channel which is corresponding to the light with different wavelengths, transmits or reflects the light through the dichroic mirror and then emits the light from one exit port, and only allows the light with specific wavelengths to pass through.
On one hand, the multi-wavelength light path selector comprises a plurality of light path channels with different wavelengths, a plurality of dichroic mirrors are arranged in the light path channels, and a plurality of lights entering the light path channels are reflected or transmitted by the dichroic mirrors and then emitted from an exit.
The multiple light path channels with different wavelengths can send the excitation light with multiple specific wavelengths to the same exit port of the light path selector according to the light path channels with the corresponding wavelengths.
In some preferred modes, the dichroic mirror closest to the exit port transmits light in the corresponding optical path to the exit port and reflects light from the other optical path to the exit port.
As shown in FIG. 1, dichroic mirror A, which is closest to the exit port1Transmits the light a in the corresponding optical path channel for the pair to the exit port, and reflects the light B, C, D from the other optical path channel to the exit port.
In some preferred embodiments, the light in the other optical path is reflected to the next dichroic mirror via the corresponding dichroic mirror, and the next dichroic mirror reflects the light reflected by the previous dichroic mirror until entering the dichroic mirror closest to the exit port, and reflects the light from the dichroic mirror closest to the exit port.
As shown in FIG. 1, light B, C, D in the other light path passes through the corresponding dichroic mirror B1、C1、D1Reflected to the next dichroic mirror A1、B1、C1Next dichroic mirror a1、B1、C1The light reflected by the previous dichroic mirror enters the dichroic mirror A closest to the exit port1From dichroic mirror A closest to the exit opening1Reflected to the exit port.
Dichroic mirrors are divided into high-pass dichroic mirrors and short-pass dichroic mirrors, and have different cut-off wavelengths due to differences in respective optical properties. When light is incident on the dichroic mirror at an angle of 45 degrees, the high-pass dichroic mirror has high reflectivity for light beams below a cut-off wavelength and high transmittance for light beams above the cut-off wavelength; the short-wave pass dichroic mirror has high reflectivity for light beams above a cut-off wavelength and high transmittance for light beams below the cut-off wavelength; we use filters to allow light within a specific wavelength range to pass.
The number of dichroic mirrors is the same as the number of optical path channels, and the cutoff wavelength is determined according to the wavelength of the excitation light or emission light passing through the optical path channels, and the combination of dichroic mirrors is various, for example, as shown in fig. 1, the wavelength of the excitation light a is a; the wavelength of the exciting light B is B; the wavelength of the excitation light C is C; the wavelength of the excitation light D is D; a > b > c > d > n.
In the optical path of the excitation light, the excitation light A is filtered by a filter having a wavelength range including a, and then a high-pass dichroic mirror A is used1Filtering the excitation light A, wherein the cut-off frequency of the filter is less than a and more than b, and the high-wave-pass dichroic mirror passes the excitation light A to the exit port; the excitation light B is filtered by a filter having a wavelength range B, and then a short-wave-pass dichroic mirror B is used1Filtering the excitation light B with a cut-off frequency less than B and greater than c, the short-wave-pass dichroic mirror B1Reflecting excitation light B to high-pass dichroic mirror A1High-pass dichroic mirror A1Reflecting the excitation light B to an exit port; the excitation light C is filtered by a filter having a wavelength range C, and then a short-wave-pass dichroic mirror C is used1Filtering the excitation light C with a cut-off frequency smaller than C and greater than d, the short-wave-pass dichroic mirror C1Reflected excitation light C to short-wave pass dichroic mirror B1Short wave pass dichroic mirror B1By excitation light C to high-pass dichroic mirror A1High-pass dichroic mirror A1Reflecting the excitation light C to an exit port; the excitation light D is filtered through a filter having a wavelength range D, and then a short-wave-pass dichroic mirror D is used1Filtering the excitation light D with a cut-off frequency smaller than D, the short-wave-pass dichroic mirror D1Reflected excitation light D to short wave pass dichroic mirror C1Short wave pass dichroic mirror C1By exciting light D to short-wave pass dichroic mirror B1Short wave pass dichroic mirror B1By excitation light D to high-pass dichroic mirror A1High-pass dichroic mirror A1The excitation light D is reflected to the exit port.
Alternatively, as shown in fig. 2, the short-wave pass dichroic mirror is replaced with a high-wave pass dichroic mirror, the high-wave pass dichroic mirror is replaced with a short-wave pass dichroic mirror, and the exit port is provided at the short-wave pass dichroic mirror in the optical path channel of the excitation light.
Through the light path channel, light in different light path channels is emitted from the same exit port and is sent into the PCR reactor through one optical fiber, the light is not required to be sent into the PCR reactor through a plurality of optical fibers respectively, and different light path channels are not required to be selected through rotation of a mechanical device and are sent into the PCR reactor through one optical fiber. The cost is reduced, and the structure of the optical path selector is simplified.
In some preferred modes, a filter is arranged in the light path channel.
The filter allows light in a specific wavelength range to pass through, and a narrow-band filter is used for minimizing the wavelength range of the passing light; the filter is used for filtering the exciting light and removing the stray light. Because the frequency of exciting light in different light path channels is different, the light filter is used for ensuring that only light with corresponding wavelength can pass through the light path channels; the filter can filter the designed wavelength only when it makes an angle of 90 degrees (+ -5 degrees) with the optical path, otherwise the wrong wavelength will be transmitted, the designed incident angle of the dichroic mirror is 45 degrees, and the cut-off light can be reflected from 90 degrees when it makes an angle of 45 degrees with the optical path in the optical path channel.
In some preferred forms, the filters of different light path channels may only pass light in that light path channel.
In some preferred forms, the optical path selector is a housing provided with a duct and an aperture, and the filter and the dichroic mirror are fixed in the optical path selector housing.
Or the light path selector is a box body provided with optical fibers and holes, and the optical filter and the dichroic mirror are fixed on the light path selector box body; the light transmission mode is various, as long as the light in different light path channels can be emitted from one exit port.
In some preferred modes, the light path selector is provided with a light path pipeline, a filter mounting hole and a dichroic mirror mounting hole, the filter is fixedly mounted in the filter mounting hole at an angle of 90 degrees with the light path pipeline, and the dichroic mirror is fixedly mounted in the dichroic mirror mounting hole at an angle of 45 degrees with the light path pipeline; a set of optical conduits, filters and dichroic mirrors correspond to an optical path.
As shown in fig. 3, the red light path channel, 645 ± 10nm narrow band pass filter, 635nm high pass dichroic mirror correspond to the red light path channel.
In some preferred modes, the optical path pipeline of one optical path channel is communicated with the filter mounting hole, the optical path pipeline, the filter mounting hole and the dichroic mirror mounting hole.
The light path selector box body is connected with the LED, the photoelectric detector and the optical fiber.
The LED is connected with the red light path pipeline through an LED interface, and the LED interface is embedded into the red light path pipeline with the same diameter as the LED interface and is fixed through a screw hole; the photoelectric detector is connected with the light path pipeline through a photoelectric detector interface, and the photoelectric detector interface is embedded into the red light path pipeline with the same diameter as the photoelectric detector interface and is fixed through a screw hole; the optical fiber is connected with the incident port or the emergent port through the optical fiber interface, and the optical fiber interface is embedded into the incident port or the emergent port with the same diameter as the optical fiber interface and is fixed through a screw hole.
Utility model's advantage lies in:
1. the exciting light with different light paths can be sent into the reaction device by using one optical fiber, so that the cost is reduced, and the use is easy.
2. The light path has simple structure, small volume and easy manufacture.
Drawings
FIG. 1 is a schematic view of the optical path channel of the present invention
FIG. 2 is another schematic diagram of the optical path channel of the present invention
Fig. 3 is a schematic view of the optical path selector of the present invention, which is a white LED1 in the figure; 2: a white LED; 3: a white LED; 4: a white LED; 5: a red light path conduit; 6: a blue optical path conduit; 7: a green light path pipe; 8: a yellow-green light path pipeline; 9: a filter mounting hole; 10: a 645nm narrow band filter; 11: a 635nm high-pass dichroic mirror; 12: a 470nm narrow band filter; 13: a 485nm high-wave-pass dichroic mirror; 14: a 520nm narrow-band filter; 15: a 535nm high-pass dichroic mirror; 16: a 570nm narrow-band filter; 17: a 585nm high-pass dichroic mirror; 18: an optical fiber; 19: an exit port; 20: an optical fiber interface; 21: an LED interface;
Detailed Description
Example 1:
the light path channels of the exciting light path selector are 4, which are respectively a red channel, a blue channel, a green channel and a yellow channel. The yellow channel, the green channel, the blue channel and the red channel are arranged from top to bottom.
Be equipped with white LED1 in the red passageway, the wavelength range includes 645nm narrowband filter 10, red light path pipeline 5, white LED1 passes through LED interface 31 and connects red light path pipeline 5, and LED interface 31 imbeds in the red light path pipeline 5 the same with LED interface 31 diameter to it is fixed through the screw hole. And light leakage between the LED interface 31 and the red light path pipe 5 is avoided. Filter mounting holes 9 are formed between the red light path pipelines 5, and 645nm narrow-band filters 10 in the wavelength range are fixedly mounted in the filter mounting holes 9 at an angle of 90 degrees with the light path pipelines. The white LED1 emits light in the range of 390nm to 780nm, and after being filtered by the narrow band pass filter 10 having a wavelength range of 645nm, excitation light having a wavelength of 645nm can pass through.
The blue channel is internally provided with a white LED2, the wavelength range comprises a 470nm narrow-band filter 12 and a blue light path pipeline 6, the white LED2 is connected with the blue light path pipeline 6 through an LED interface 31, and the LED interface 31 is embedded into the blue light path pipeline 6 with the same diameter as the LED interface 31 and is fixed through a screw hole. And light leakage between the LED interface 31 and the blue light path pipe 6 is avoided. An optical filter mounting hole 9 is arranged between the blue light path pipelines 6, and a 470nm narrow-band optical filter 12 in a wavelength range is fixedly mounted in the optical filter mounting hole 9 at an angle of 90 degrees with the light path pipelines. The white LED2 emits light in the range of 390nm to 780nm, and after being filtered by the narrowband filter 14 having a wavelength range including 470nm, excitation light having a wavelength of 470nm can pass through.
Be equipped with white LED3 in the green passageway, the wavelength range includes 520nm narrowband optical filter 14, green light path pipeline 7, and white LED3 passes through LED interface 31 and connects green light path pipeline 7, and LED interface 31 imbeds in the same green light path pipeline 7 with LED interface 31 diameter to it is fixed through the screw hole. And light leakage between the LED interface 31 and the green light path pipeline 7 is avoided. An optical filter mounting hole 9 is formed between the green light path pipelines 7, and a narrow-band optical filter with the wavelength range of 635nm is fixedly mounted in the optical filter mounting hole 9 at an angle of 90 degrees with the light path pipelines. The white LED3 emits light in the 390nm to 780nm range, and after being filtered by the narrow band filter 18 with a wavelength range including 520nm, excitation light with a wavelength of 520nm can pass through.
Be equipped with white LED4 in the yellow green passageway, the wavelength range includes 5570nm narrowband optical filter 16, yellow green light path pipeline 8, and white LED4 passes through LED interface 31 and connects yellow green light path pipeline 8, and LED interface 31 imbeds in the yellow green light path pipeline 8 the same with LED interface 31 diameter to it is fixed through the screw hole. And light leakage between the LED interface 31 and the yellow-green light path pipeline 8 is avoided. Filter mounting holes 9 are arranged between the yellow-green light path pipelines 8, and the narrow-band filters 22 with the wavelength ranges of 570nm are fixedly mounted in the filter mounting holes 9 at an angle of 90 degrees with the light path pipelines. The white LED4 emits light in the range of 390nm to 780nm, and after being filtered by the narrow band filter 22 having a wavelength range including 570nm, excitation light having a wavelength of 570nm can pass through.
A dichroic mirror mounting hole and an exit port 19 are further arranged in the excitation light path selector, and the red light path pipeline 5, the blue light path pipeline 6, the green light path pipeline 7 and the yellow-green light path pipeline 8 are all led into the dichroic mirror mounting hole. The dichroic mirror mounting hole exits through an exit port 19.
The dichroic mirror mounting hole is provided with a fixing piece at the inlet of the red light path pipeline 5, the fixing piece fixes the 635nm high-wave-pass dichroic mirror 11, and the fixed 635nm high-wave-pass dichroic mirror 11 forms an angle of 45 degrees with the red light path pipeline 5. The 635nm high-pass dichroic mirror 11 passes the 645nm excitation light to the exit port 19.
The dichroic mirror mounting hole is provided with a fixing piece at the inlet of the blue light path pipeline 6, the fixing piece fixes the 480nm high-wave-pass dichroic mirror 13, and the fixed 485nm high-wave-pass dichroic mirror 13 forms an angle of 45 degrees with the blue light path pipeline 6. The 485nm high-pass dichroic mirror 13 reflects the excitation light at 470nm to the 635nm high-pass dichroic mirror 11,635nm high-pass dichroic mirror 11 reflects the excitation light at 470nm to the exit port 19.
The dichroic mirror mounting hole is provided with a fixing member at the inlet of the green light path pipe 7, the fixing member fixes the 535nm high-wave-pass dichroic mirror 15, and the fixed 535nm high-wave-pass dichroic mirror 15 forms an angle of 45 degrees with the green light path pipe 7. The 535nm high-pass dichroic mirror 15 reflects the 520nm excitation light to the 485nm high-pass dichroic, the 485nm high-pass dichroic mirror 13 passes the 520nm excitation light to the 635nm high-pass dichroic mirror 11, and the 635nm high-pass dichroic mirror 11 reflects the 520nm excitation light to the exit port 19.
The dichroic mirror mounting hole sets up the mounting in yellow green light path pipeline 8 department of letting in, and the 585nm high-wave that the mounting is fixed leads to dichroic mirror 17, and the 585nm high-wave that fixes leads to dichroic mirror 17 and yellow green light path pipeline 8 and becomes 45 degrees angles. The 585nm high-pass dichroic mirror 17 reflects the 570nm excitation light to the 535nm high-pass dichroic mirror 15, the 535nm high-pass dichroic mirror 15 passes the 570nm excitation light to the 485nm high-pass dichroic mirror, the 485nm high-pass dichroic mirror 13 passes the 570nm excitation light to the 635nm high-pass dichroic mirror 11, and the 635nm high-pass dichroic mirror 11 reflects the 570nm excitation light to the exit port 19.
The optical fiber 18 is connected with the exit port 19 through an optical fiber interface 30, and the optical fiber interface 30 is embedded into the exit port 19 with the same diameter as the optical fiber interface 20 and fixed through a screw hole. And light leakage between the optical fiber interface 20 and the exit port 19 is avoided.
Claims (8)
1. The utility model provides a multi-wavelength light path selector, multi-wavelength light path selector include the different light path passageway of a plurality of wavelengths, and a plurality of light path passageways correspond a plurality of dichroic mirrors respectively, its characterized in that: the plurality of lights entering the light path channel are reflected or transmitted by the dichroic mirror and then exit from an exit port.
2. A multi-wavelength optical path selector as claimed in claim 1, wherein: the dichroic mirror closest to the exit port transmits light in the corresponding optical path channel to the exit port, and reflects light from the other optical path channels to the exit port.
3. A multi-wavelength optical path selector as claimed in claim 1, wherein: the light in the other optical path channels is reflected to the next dichroic mirror through the corresponding dichroic mirror, and the light reflected by the next dichroic mirror through the previous dichroic mirror enters the dichroic mirror closest to the exit port and is reflected to the exit port by the dichroic mirror closest to the exit port.
4. A multi-wavelength optical path selector as claimed in claim 1, wherein: the light path channel is provided with a light filter.
5. A multi-wavelength optical path selector as claimed in claim 2, wherein: in the following steps: filters of different light path channels can only pass light in that light path channel.
6. A multi-wavelength optical path selector as claimed in claim 4, wherein: the light path selector is a box body provided with a pipeline and a hole, and the optical filter and the dichroic mirror are fixed in the light path selector box body.
7. A multi-wavelength optical path selector as claimed in claim 3 or 6, wherein: the light path selector is provided with a light path pipeline, a light filter mounting hole and a dichroic mirror mounting hole, the light filter is fixedly mounted in the light filter mounting hole at an angle of 90 degrees with the light path pipeline, and the dichroic mirror is fixedly mounted in the dichroic mirror mounting hole at an angle of 45 degrees with the light path pipeline; a set of optical conduits, filters and dichroic mirrors correspond to an optical path.
8. A multi-wavelength optical path selector as claimed in claim 7, wherein: and a light filter mounting hole, a light path pipeline, a light filter mounting hole, a dichroic mirror mounting hole and an exit port are arranged among the light path pipelines of one light path channel and communicated with each other.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922001234.9U CN211554382U (en) | 2019-11-19 | 2019-11-19 | Multi-wavelength light path selector |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922001234.9U CN211554382U (en) | 2019-11-19 | 2019-11-19 | Multi-wavelength light path selector |
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| Publication Number | Publication Date |
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| CN211554382U true CN211554382U (en) | 2020-09-22 |
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| CN201922001234.9U Active CN211554382U (en) | 2019-11-19 | 2019-11-19 | Multi-wavelength light path selector |
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