CN217085405U - Multi-channel LED light source for fluorescence microscopic imaging - Google Patents
Multi-channel LED light source for fluorescence microscopic imaging Download PDFInfo
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- CN217085405U CN217085405U CN202220888936.2U CN202220888936U CN217085405U CN 217085405 U CN217085405 U CN 217085405U CN 202220888936 U CN202220888936 U CN 202220888936U CN 217085405 U CN217085405 U CN 217085405U
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Abstract
The utility model belongs to the technical field of the LED lighting device, a multichannel LED light source for fluorescence is micro-imaged is provided, include: the LED light emitting device is arranged in each LED channel and used for emitting LED light beams and transmitting the LED light beams through optical fibers; each condensing lens corresponds to one LED channel, is arranged opposite to the optical fiber output end of the LED channel and is used for converging and collimating the LED light beams output by the LED channels; the light-emitting diode comprises a plurality of light filters, a light-emitting diode and a light-emitting diode, wherein each light filter corresponds to a condensing lens and is used for filtering an LED light beam passing through the condensing lens; and the at least one dichroic mirror is obliquely arranged relative to the one or more optical filters and is used for reflecting and/or transmitting the LED light beams passing through the optical filters and then combining and outputting the LED light beams. The utility model provides a multichannel LED light source can provide high-quality LED light beam, realizes the independent assortment of the LED light source of different wavelength and power, satisfies the high convenient degree demand of changing the LED light source, satisfies the different demands that fluorescence arouses under the different scenes.
Description
Technical Field
The utility model belongs to the technical field of the LED lighting device, especially, relate to a multichannel LED light source for fluorescence is micro-imaged.
Background
The LED light source has the characteristics of energy conservation, high intensity, long service life, good controllability, stable spectral output and the like, not only becomes a preferred light source in the field of common illumination, but also develops some professional illumination applications. For example, LED light sources in fluorescence microscopes can maintain the compositional validity of the sample to be measured, particularly for imaging and preservation of sensitive samples. Therefore, the LED fluorescence microscope is widely used in the study of single-cell and multi-cell biological specimens.
In LED fluorescence microscopes, a multi-color LED light source is usually used, and the existing multi-color LED light source generally has at least one of the following problems: collimation and homogenization are not carried out, and some collimating and homogenization but beam combination is not carried out, so that the method cannot be applied to a professional fluorescence microscope; no filtering results in high background noise; the power control can not be carried out, and the multi-wavelength combination is difficult to realize according to the requirement; the light emitting mode is monotonous, only continuous light can be emitted but pulse light cannot be emitted, and the periodic frequency of the pulse light cannot be controlled; cannot be triggered externally; the LED light source is difficult to replace after being packaged.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a multichannel LED light source for fluorescence is micro-imaged to at least, solve one of above-mentioned problem.
In order to achieve the above purpose, the technical scheme of the utility model is as follows:
the utility model provides a multichannel LED light source for fluorescence is micro-imaged, include: the LED light emitting device is arranged in each LED channel and used for emitting LED light beams and transmitting the LED light beams through optical fibers; each condensing lens corresponds to one LED channel, is arranged opposite to the optical fiber output end of the LED channel and is used for converging and collimating the LED light beams output by the LED channels; each optical filter corresponds to one condensing lens and is used for filtering the LED light beams passing through the condensing lens; and the at least one dichroic mirror is obliquely arranged relative to one or more optical filters and is used for reflecting and/or transmitting the LED light beams passing through the optical filters and then combining and outputting the LED light beams.
Further, the plurality of LED channels includes: the LED light path structure comprises a 1 st LED channel, a 2 nd LED channel and a 3 rd LED channel, wherein the light paths of the 2 nd LED channel and the 3 rd LED channel are parallel, and the light paths of the 1 st LED channel and the 2 nd LED channel are mutually orthogonal.
Further, the number of the at least one dichroic mirror is 2; the 1 st dichroic mirror is positioned at the intersection of the light paths of the 1 st LED channel and the 2 nd LED channel and is obliquely arranged relative to the optical filters corresponding to the 1 st LED channel and the 2 nd LED channel respectively; the 2 nd dichroic mirror is positioned at the intersection of the light paths of the 1 st LED channel and the 3 rd LED channel and is obliquely arranged relative to the optical filters corresponding to the 1 st LED channel and the 3 rd LED channel respectively.
Further, the LED light beam of the 1 st LED channel passes through the corresponding optical filter and then sequentially transmits through the 1 st dichroic mirror and the 2 nd dichroic mirror; the LED light beams of the 2 nd LED channel pass through the corresponding optical filters, are reflected by the 1 st dichroic mirror and then are transmitted by the 2 nd dichroic mirror; and the LED light beam of the 3 rd LED channel passes through the corresponding optical filter and then is reflected by the 2 nd dichroic mirror.
Furthermore, the multi-channel LED light source for fluorescence microscopic imaging further comprises a light outlet which is arranged opposite to the at least one dichroic mirror, and the LED light beams passing through the optical filters are reflected and/or transmitted and then combined to be output through the light outlet.
Furthermore, every LED light emitting device detachable sets up in the LED passageway that corresponds, satisfies the high convenient degree demand of changing the LED light source.
Further, the LED light emitting devices in different LED channels emit different LED light wavelengths, powers and spatial structures.
Further, the LED light source in each of the LED light emitting devices is a single color LED.
Further, each of the LED light emitting devices emits continuous light or pulsed light.
Further, the condensing lens is a plano-convex lens.
The embodiment of the utility model provides a from three channel LED light sources of group for fluorescence is micro-imaged has following beneficial effect:
(1) under different application scenes, according to different requirements of fluorescence excitation, a user can quickly and independently assemble the LED light sources, so that the free combination of the LED light sources with different wavelengths and different powers is realized, high-quality light beams are output, the requirement of high convenience for replacing the LED light sources is met, and different requirements of fluorescence excitation under different application scenes are met;
(2) the LED light beams of each channel are collimated and homogenized by arranging the condensing lens on each LED channel, so that the light beam quality is improved;
(3) the LED light beams of each channel are filtered by arranging the filter plate, unnecessary wave bands are removed, and background noise is reduced;
(4) the dichroic mirrors are arranged to combine the LED light beams of each channel, so that specific requirements such as fluorescence excitation are met;
(5) the user can conveniently control the light intensity of the LEDs with different wavelengths in real time so as to freely control the wavelength combination;
(6) the LED lighting mode is diversified, and has a continuous mode and a pulse mode so as to be suitable for various application scenes.
Drawings
Fig. 1 is a schematic structural diagram of a multi-channel LED light source for fluorescence microscopy imaging according to an embodiment of the present invention.
Reference numbers in the drawings of the specification:
101-1 st LED channel, 102-2 nd LED channel, 103-3 rd LED channel, 201-condenser lens I, 202-condenser lens II, 203-condenser lens III, 301-optical filter I, 302-optical filter II, 303-optical filter III, 401-1 st dichroic mirror, 402-2 nd dichroic mirror, and 500-light outlet.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings of the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.
The embodiment of the utility model provides a multichannel LED light source for fluorescence is micro-imaged. Fig. 1 is a schematic structural diagram of a multi-channel LED light source for fluorescence microscopy imaging according to an embodiment of the present invention. The multi-channel LED light source of the present embodiment is exemplified by including 3LED channels, and the inventive idea of the present invention is described. As shown in fig. 1, the multi-channel LED light source includes: the three LED channels are a 1 st LED channel 101, a 2 nd LED channel 102, and a 3 rd LED channel 103. And an LED light emitting device is arranged in each LED channel and used for emitting LED light beams and transmitting the LED light beams through optical fibers. The LED light emitting device may be a single color LED device, and in the present embodiment, the LED light source in the LED light emitting device is a single color LED. In the present embodiment, the LED light emitting device may include: LED light source, adapter and multimode fiber bundle. The adapter is respectively connected with the LED light source and the multimode fiber bundle and is used for coupling the LED light emitted by the LED light source into the multimode fiber bundle for transmission. Preferably, each LED light emitting device is arranged in the corresponding LED channel in a manner of easy disassembly, so that the requirement of high convenience for replacing the LED light source is met.
And a condensing lens is arranged outside the tail end (namely the optical fiber output end) of each LED channel and is used for converging, collimating and homogenizing the LED light beams output by the LED channels, so that the light beam quality is improved. In the present embodiment, the 1 st LED channel 101, the 2 nd LED channel 102 and the 3 rd LED channel 103 are respectively provided with a condenser lens one 201, a condenser lens two 202 and a condenser lens three 203. In this embodiment, the condenser lens may be a plano-convex lens.
And the outer side of each condensing lens is correspondingly provided with an optical filter which is used for filtering the LED light beams passing through the condensing lens, eliminating unnecessary wave bands and reducing background noise. In this embodiment, the first filter 301 is disposed outside the first condenser lens 201, the second filter 302 is disposed outside the second condenser lens 202, and the third filter 303 is disposed outside the third condenser lens 203.
Thus, each LED channel with its corresponding condenser lens and filter constitutes a set of optical paths. In this embodiment, the light paths of the 2 nd LED channel 102 and the 3 rd LED channel 103 are parallel, and the light paths of the 1 st LED channel 101 and the 2 nd LED channel 102 are orthogonal to each other, which is beneficial to reducing the number of elements for changing the light propagation direction, and effectively utilizing the space, so that the system is more compact and simpler, and the system error is reduced. In this embodiment, the multi-channel LED light source further comprises two dichroic mirrors. The 1 st dichroic mirror 401 is located at the intersection of the light paths of the 1 st LED channel 101 and the 2 nd LED channel 102, and is respectively arranged in an inclined manner relative to the optical filters in the 1 st LED channel 101 and the 2 nd LED channel 102; the 2 nd dichroic mirror 402 is located at the intersection of the optical paths of the 1 st LED channel 101 and the 3 rd LED channel 103, and is obliquely arranged relative to the optical filters in the 1 st LED channel 101 and the 3 rd LED channel 103, respectively.
The light paths of the three LED channels are respectively as follows: the LED light beam of the 1 st LED channel 101 passes through the first optical filter 301 and then sequentially transmits through the 1 st dichroic mirror 401 and the 2 nd dichroic mirror 402; the LED light beam of the 2 nd LED channel 102 passes through the second optical filter 302, then is reflected by the 1 st dichroic mirror 401 and then is transmitted by the 2 nd dichroic mirror 402; the LED beam of the 3 rd LED channel 103 passes through filter three 303 and is reflected by the 2 nd dichroic mirror 402. Finally, the three beams of light are output through the light outlet 500.
In the present embodiment, the LED light emitting devices in different LED channels emit different LED light wavelengths, powers and spatial structures. The LED light beams of different channels are output by arranging the plurality of dichroic mirrors, so that specific requirements such as fluorescence excitation are met.
Further, the multi-channel LED light source for fluorescence microscopy provided by the present embodiment has diversified light emitting modes, each LED light emitting device emits continuous light or pulsed light, and the period frequency of the pulsed light can be controlled by TTL electrical signal.
The embodiment of the utility model provides a multichannel LED light source for fluorescence is micro-imaged under the applied scene of difference, according to fluorescence arouses different demands, the user can swiftly independently assemble the LED light source, realizes the independent combination of the LED light source of different wavelength and power, exports high-quality light beam, satisfies the high convenient degree demand of changing the LED light source, satisfies the different demands that fluorescence arouses under the different applied scene.
The foregoing has described in detail preferred embodiments of the present invention. It should be understood that numerous modifications and variations can be devised by those skilled in the art in light of the teachings of the present invention without undue experimentation. Therefore, the technical solutions that can be obtained by a person skilled in the art through logic analysis, reasoning or limited experiments based on the prior art according to the concepts of the present invention should be within the scope of protection defined by the claims.
Claims (10)
1. A multi-channel LED light source for fluorescence microscopy imaging, comprising:
the LED light emitting device is arranged in each LED channel and used for emitting LED light beams and transmitting the LED light beams through optical fibers;
each condensing lens corresponds to one LED channel, is arranged opposite to the optical fiber output end of the LED channel and is used for converging and collimating the LED light beams output by the LED channels;
the light-emitting diode comprises a plurality of light filters, a plurality of light-emitting diodes and a plurality of light-collecting lenses, wherein each light filter corresponds to one light-collecting lens and is used for filtering the LED light beams passing through the light-collecting lenses;
and the at least one dichroic mirror is obliquely arranged relative to one or more optical filters and is used for reflecting and/or transmitting the LED light beams passing through the optical filters and then combining and outputting the LED light beams.
2. The multi-channel LED light source for fluorescence microscopy imaging as defined in claim 1, wherein the plurality of LED channels comprises: the LED light path structure comprises a 1 st LED channel, a 2 nd LED channel and a 3 rd LED channel, wherein the light paths of the 2 nd LED channel and the 3 rd LED channel are parallel, and the light paths of the 1 st LED channel and the 2 nd LED channel are mutually orthogonal.
3. The multi-channel LED light source for fluorescence microscopy according to claim 2,
the number of the at least one dichroic mirror is 2; wherein, the first and the second end of the pipe are connected with each other,
the 1 st dichroic mirror is positioned at the intersection of the light paths of the 1 st LED channel and the 2 nd LED channel and is obliquely arranged relative to the optical filters corresponding to the 1 st LED channel and the 2 nd LED channel respectively;
the 2 nd dichroic mirror is positioned at the intersection of the light paths of the 1 st LED channel and the 3 rd LED channel and is obliquely arranged relative to the optical filters corresponding to the 1 st LED channel and the 3 rd LED channel respectively.
4. The multi-channel LED light source for fluorescence microscopy according to claim 3,
the LED light beams of the 1 st LED channel pass through the corresponding optical filters and then sequentially transmit through the 1 st dichroic mirror and the 2 nd dichroic mirror;
the LED light beams of the 2 nd LED channel pass through the corresponding optical filters, are reflected by the 1 st dichroic mirror and then are transmitted by the 2 nd dichroic mirror;
and the LED light beam of the 3 rd LED channel passes through the corresponding optical filter and then is reflected by the 2 nd dichroic mirror.
5. The multi-channel LED light source for fluorescence microscopy according to claim 1, further comprising a light exit port disposed opposite the at least one dichroic mirror, through which the combined LED light beams after reflection and/or transmission of the LED light beams passing through the respective filters are output.
6. The multi-channel LED light source for fluorescence microscopy according to claim 1, wherein each of the LED light emitting devices is removably disposed in a corresponding LED channel.
7. The multi-channel LED light source for fluorescence microscopy imaging as defined in claim 1 wherein the LED light emitting devices in different LED channels emit different LED light wavelengths, powers and spatial structures.
8. The multi-channel LED light source for fluorescence microscopy according to claim 1, wherein the LED light source in each of the LED light emitting devices is a single color LED.
9. The multi-channel LED light source for fluorescence microscopy according to claim 1, wherein each of the LED light emitting devices emits continuous or pulsed light.
10. The multi-channel LED light source for fluorescence microscopy according to claim 1, wherein the condenser lens is a plano-convex lens.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220888936.2U CN217085405U (en) | 2022-04-18 | 2022-04-18 | Multi-channel LED light source for fluorescence microscopic imaging |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220888936.2U CN217085405U (en) | 2022-04-18 | 2022-04-18 | Multi-channel LED light source for fluorescence microscopic imaging |
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| CN217085405U true CN217085405U (en) | 2022-07-29 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202220888936.2U Active CN217085405U (en) | 2022-04-18 | 2022-04-18 | Multi-channel LED light source for fluorescence microscopic imaging |
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