CN217085406U - Multi-channel LED light source for fluorescence microscopic imaging - Google Patents
Multi-channel LED light source for fluorescence microscopic imaging Download PDFInfo
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- CN217085406U CN217085406U CN202221007610.0U CN202221007610U CN217085406U CN 217085406 U CN217085406 U CN 217085406U CN 202221007610 U CN202221007610 U CN 202221007610U CN 217085406 U CN217085406 U CN 217085406U
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Abstract
The utility model provides a multichannel LED light source for fluorescence is micro-imaged, include: four sets of LED channels, each set of LED channels includes: the LED light emitting device is used for emitting an LED light beam and transmitting the LED light beam through an optical fiber; the condensing lens is used for converging and collimating the LED light beam emitted by the LED light emitting device; the optical filter is used for filtering the LED light beam passing through the condensing lens; the first dichroic mirror is used for transmitting the LED light beams from the first LED channel and reflecting the LED light beams from the third LED channel and then combining the beams to irradiate the third dichroic mirror; the second dichroic mirror is used for transmitting the LED light beams from the fourth LED channel and reflecting the LED light beams from the second LED channel and then combining the beams to emit to the third dichroic mirror; and the third dichroic mirror is used for transmitting the combined light from the first dichroic mirror and reflecting the combined light from the second dichroic mirror to output combined light. The utility model discloses a multichannel LED light source can obtain high quality polychrome light beam, satisfies fluorescence excitation's specific demand.
Description
Technical Field
The utility model belongs to the technical field of the LED lighting device, especially, relate to a multichannel LED light source for fluorescence is micro-imaged.
Background
The LED light source has the characteristics of energy conservation, high intensity, long service life, good controllability, stable spectral output and the like, not only becomes a preferred light source in the field of common illumination, but also develops some professional illumination applications. For example, LED light sources in fluorescence microscopes can maintain the compositional validity of the sample to be measured, particularly for imaging and preservation of sensitive samples. Therefore, the LED fluorescence microscope is widely used in the study of single-cell and multi-cell biological specimens.
In LED fluorescence microscopes, a multi-color LED light source is usually used, and multi-color light is combined to obtain high-quality excited fluorescence satisfying specific requirements. However, the existing LED light source, especially the multi-channel light source with three or more colors of light, generally has at least one of the following problems: the light path design is complex, and the free combination of light sources with multiple wavelengths and different powers is difficult to realize according to the requirements; the different requirements of the fluorescent laser in different application scenes cannot be met.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a multichannel LED light source for fluorescence is micro-imaged to at least, solve one of above-mentioned problem.
In order to achieve the above purpose, the technical scheme of the utility model is as follows:
the utility model provides a multichannel LED light source for fluorescence is micro-imaged, include:
four groups of LED channels, which are respectively marked as a first LED channel, a second LED channel, a third LED channel, and a fourth LED channel, wherein the first LED channel and the second LED channel are arranged in parallel, the third LED channel and the fourth LED channel are arranged in parallel, the first LED channel and the third LED channel are arranged orthogonally, and each group of LED channels includes: the LED light emitting device is used for emitting an LED light beam and transmitting the LED light beam through an optical fiber; the condensing lens is arranged opposite to the optical fiber output end of the LED light emitting device and is used for converging and collimating the LED light beam emitted by the LED light emitting device; the optical filter is arranged opposite to the condensing lens and is used for filtering the LED light beam passing through the condensing lens;
the first dichroic mirror is arranged at the intersection of the light paths of the first LED channel and the third LED channel, is obliquely arranged relative to the optical filter of the first LED channel and the optical filter of the third LED channel respectively, and is used for transmitting the LED light beams from the first LED channel and reflecting the LED light beams from the third LED channel and then combining the light beams to emit to the third dichroic mirror;
the second dichroic mirror is arranged at the intersection of the light paths of the second LED channel and the fourth LED channel, is obliquely arranged relative to the optical filter of the second LED channel and the optical filter of the fourth LED channel, and is used for transmitting the LED light beams from the fourth LED channel and reflecting the LED light beams from the second LED channel and then combining the light beams to emit to the third dichroic mirror;
the third dichroic mirror is arranged at the intersection of the light paths of the first LED channel and the fourth LED channel, is obliquely arranged relative to the optical filter of the first LED channel and the optical filter of the fourth LED channel, and is used for transmitting the combined light from the first dichroic mirror and reflecting the combined light from the second dichroic mirror and outputting the combined light.
Further, the multi-channel LED light source for fluorescence microscopy according to an embodiment of the present invention further includes a light outlet, which is disposed opposite to the third dichroic mirror, and through which the light beam combined by the third dichroic mirror is output.
Further, the four groups of LED channels, the first dichroic mirror, the second dichroic mirror and the third dichroic mirror are arranged in a first chamber; the multi-channel LED light source for fluorescence microscopy imaging further comprises at least one second chamber, the second chamber is arranged side by side with the first chamber, and the second chamber is provided with the same optical elements and arrangement as the first chamber.
Further, the multi-channel LED light source for fluorescence microscopy according to the embodiment of the invention further comprises a fourth dichroic mirror, which is obliquely arranged relative to the light outlet of one of the first chamber and the second chamber and is used for transmitting and transmitting the light beams from the corresponding light outlet.
Further, the multi-channel LED light source for fluorescence microscopy according to an embodiment of the present invention further includes a reflector, which is disposed obliquely with respect to the light exit port of the first chamber and the second chamber where the fourth dichroic mirror is not disposed, and is configured to reflect and transmit the light beam from the corresponding light exit port to the fourth dichroic mirror for further reflection and transmission.
Furthermore, the multi-channel LED light source for fluorescence microscopy according to an embodiment of the present invention further includes a second condenser lens, disposed opposite to the fourth dichroic mirror, for focusing a combined light beam transmitted through the fourth dichroic mirror and a combined light beam reflected through the fourth dichroic mirror by the second condenser lens.
Further, the multi-channel LED light source for fluorescence microscopic imaging according to the embodiment of the invention further comprises an optical fiber outlet, and the light beam focused by the second condenser lens is coupled out through the optical fiber at the optical fiber outlet.
Further, each of the LED light emitting devices emits LED light having different wavelengths, powers and spatial structures.
Further, each of the LED light emitting devices may be detachably disposed in the corresponding LED channel or integrally disposed with the corresponding condensing lens and filter.
Further, each of the LED light emitting devices emits continuous light or pulsed light.
The embodiment of the utility model provides a multichannel LED light source for fluorescence is micro-imaged has following beneficial effect:
(1) the utility model provides a design idea of multichannel LED light source with the number of channels more than 3 (such as 4 channels, 8 channels and the like), which combines LED light beams of different light paths by utilizing a dichroic mirror, greatly increases the number of channels by arranging parallel chambers, and combines the light paths for the second time, finally obtains multichannel high-quality combined light beams, and meets the specific requirement of fluorescence excitation;
(2) under different application scenes, according to different requirements of fluorescence excitation, a user can select different LED light sources, so that the free combination of the LED light sources with different wavelengths and different powers is realized, high-quality light beams are output, and the different requirements of the fluorescence excitation under different application scenes are met;
(3) the LED light beams of each channel are collimated and homogenized by arranging the condensing lens on each LED channel, so that the light beam quality is improved;
(4) the LED light beams of each channel are filtered by arranging the filter plate, unnecessary wave bands are removed, and background noise is reduced;
(5) the LED lighting mode is diversified, and has a continuous mode and a pulse mode so as to be suitable for various application scenes.
Drawings
Fig. 1 is a front view of a schematic structural diagram of a multi-channel LED light source for fluorescence microscopy according to embodiment 1 of the present invention;
fig. 2 is a top view of a schematic structural diagram of a multi-channel LED light source for fluorescence microscopy imaging according to embodiment 2 of the present invention.
Reference numbers in the drawings of the specification:
1-a first LED channel, 2-a second LED channel, 3-a third LED channel, 4-a fourth LED channel, 101-an LED light emitting device, 102-a condenser lens, 103-a light filter, 201-a first dichroic mirror, 202-a second dichroic mirror, 203-a third dichroic mirror, 204-a fourth dichroic mirror, 301-a light outlet of a first chamber, 302-a light outlet of a second chamber, 205-a reflector, 401-a first chamber, 402-a second chamber, 500-a second condenser lens, 300-a fiber outlet.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings of the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.
Example 1
The embodiment of the utility model provides a multichannel LED light source for fluorescence is micro-imaged. Fig. 1 is a front view of a schematic structural diagram of a multi-channel LED light source for fluorescence microscopy imaging according to an embodiment of the present invention. As shown in fig. 1, the multi-channel LED light source includes: the four groups of LED channels are respectively marked as a first LED channel 1, a second LED channel 2, a third LED channel 3, and a fourth LED channel 4. The first LED channel 1 and the second LED channel 2 are arranged in parallel, the third LED channel 3 and the fourth LED channel 4 are arranged in parallel, and the first LED channel 1 and the third LED channel are arranged orthogonally.
Each set of LED channels includes: an LED light emitting device 101, a condenser lens 102, and an optical filter 103.
The LED light emitting device 101 is used for emitting LED light beams and transmitting the LED light beams through optical fibers. The LED light emitting device 101 may be a single-color LED device, and in the present embodiment, the LED light sources in the LED light emitting device 101 are single-color LEDs, and the LED light emitting devices 101 in the respective LED channels emit LED light of different wavelengths. In the present embodiment, the LED light emitting device 101 may include: LED light source, adapter and multimode fiber bundle. The adapter is respectively connected with the LED light source and the multimode fiber bundle and is used for coupling the LED light emitted by the LED light source into the multimode fiber bundle for transmission.
Preferably, each LED light emitting device 101 is disposed in the corresponding LED channel in a manner that is easy to detach, so as to meet the requirement of high convenience in replacing the LED light source. In another embodiment, each LED light emitting device 101 is integrally disposed in a corresponding LED channel with a corresponding condenser lens and filter.
And the condensing lens 102 is arranged opposite to the optical fiber output end of the LED light emitting device 101 and is used for converging, collimating and homogenizing the LED light beams emitted by the LED light emitting device and improving the light beam quality. In this embodiment, the condenser lens may be a plano-convex lens.
And the optical filter 103 is arranged opposite to the condensing lens 102 and is used for filtering the LED light beams passing through the condensing lens 102, eliminating unnecessary wave bands, reducing background noise and improving monochromaticity. In each LED channel, an LED light beam is emitted from the LED light emitting device 101, and exits through the condenser lens 102 and the filter 103 to constitute an optical path of the LED channel.
The multi-channel LED light source of this embodiment further includes three dichroic mirrors, which are respectively recorded as: first dichroic mirror 201, second dichroic mirror 202, third dichroic mirror 203. The first dichroic mirror 201 is disposed at an intersection of light paths of the first LED channel 1 and the third LED channel 3, and is respectively disposed in an inclined manner with respect to the optical filter 103 of the first LED channel 1 and the optical filter 103 of the third LED channel 3, and configured to transmit the LED light beams from the first LED channel 1 and reflect the LED light beams from the third LED channel 3 to be combined and emitted to the third dichroic mirror 203. And a second dichroic mirror 202, disposed at an intersection of optical paths of the second LED channel 2 and the fourth LED channel 4, and respectively disposed in an inclined manner with respect to the optical filter 103 of the second LED channel 2 and the optical filter 103 of the fourth LED channel 4, for transmitting the LED light beam from the fourth LED channel 4, and reflecting the LED light beam from the second LED channel 2 and then combining the light beam to the third dichroic mirror 203. And the third dichroic mirror 203 is arranged at the intersection of the light paths of the first LED channel 1 and the fourth LED channel 4, is obliquely arranged relative to the optical filter 103 of the first LED channel 1 and the optical filter 103 of the fourth LED channel 4, and is used for transmitting the combined light from the first dichroic mirror 201 and reflecting the combined light from the second dichroic mirror 202, and then combining and outputting the combined light.
In this embodiment, the multi-channel LED light source further comprises a light outlet 301. The light exit 301 is provided opposite to the third dichroic mirror 203, and the light beams combined by the third dichroic mirror 203 are output through the light exit 301.
In the present embodiment, the LED light emitting devices in different LED channels emit different LED light wavelengths, powers and spatial structures. LED light of different passageways is combined to be exported through setting up a plurality of dichroic mirrors to satisfy specific demands such as fluorescence excitation.
Further, the multi-channel LED light source for fluorescence microscopy provided by the present embodiment has diversified light emitting modes, each LED light emitting device emits continuous light or pulsed light, and the period frequency of the pulsed light can be controlled by TTL electrical signal.
The embodiment provides a four-channel LED light source, and the LED light beams of different light paths are combined by using a dichroic mirror, so that a multi-channel high-quality combined light beam is finally obtained. Under different application scenes, according to different requirements of fluorescence excitation, a user can select different LED light sources, so that the free combination of the LED light sources with different wavelengths and different powers is realized, high-quality light beams are output, and the different requirements of the fluorescence excitation under different application scenes are met.
Example 2
The embodiment of the utility model provides a multichannel LED light source for fluorescence is micro-imaged. Fig. 2 is a top view of a schematic structural diagram of a multi-channel LED light source for fluorescence microscopy imaging according to an embodiment of the present invention. In this embodiment, the multi-channel LED light source has 8 LED channels. Wherein every 4 LED channels are in one group and are arranged in one chamber. The arrangement of the LED channels and the arrangement of the dichroic mirror in a single chamber are as described in embodiment 1 of the present invention and will not be described herein. That is, two identical chambers are horizontally arranged side by side, with the different chambers being separated by a sheet, as shown in top view in FIG. 2. A fourth dichroic mirror 204 and a mirror 205 are arranged outside the two chambers. As shown in fig. 2, the fourth dichroic mirror 204 is disposed obliquely with respect to the light exit 301 of the first chamber 401, and is configured to transmit the light beam from the light exit 301 of the first chamber. The reflecting mirror 205 is disposed obliquely with respect to the light outlet 302 of the second chamber, and is used for transmitting the light beam from the light outlet 302 of the second chamber 402 to the fourth dichroic mirror 204 for reflection and transmission.
In this embodiment, the multi-channel LED light source further includes a second condenser lens 500 and a fiber outlet 300. The second condenser lens 500 is disposed opposite to the fourth dichroic mirror 204, and after the light beam transmitted through the fourth dichroic mirror 204 and the light beam reflected through the fourth dichroic mirror 204 are combined, the light beam is focused into the optical fiber coupling output of the optical fiber outlet 300 through the second condenser lens 500, so as to implement the light output of the eight-color LED.
The utility model provides an eight passageway LED light sources closes through the LED light beam that utilizes dichroic mirror to different light paths and restraints to through setting up side by side cavity greatly increased passageway quantity, and carry out the secondary to the light path and close and restraint, finally obtain the high quality of multichannel and close a beam bundle light beam, satisfy fluorescence excitation's specific demand.
Example 3
This embodiment can be obtained by expanding the number of chambers of embodiment 2, for example, expanding the number of chambers to three. The first cavity light-emitting port is provided with a dichroic mirror, the second cavity light-emitting port is provided with a dichroic mirror, the third cavity light-emitting port is provided with a dichroic mirror, the three dichroic mirrors reflect or transmit combined light corresponding to the light-emitting ports to a condensing lens for focusing under the synergistic effect, and then the combined light is output by an optical fiber outlet of the multi-channel LED light source.
The utility model provides a multichannel LED light source closes the bundle through utilizing the dichroic mirror to the LED light beam of different light paths to through setting up chamber greatly increased passageway quantity side by side, and carry out the secondary to the light path and close the bundle, the high quality that finally obtains the multichannel closes a bundle light beam, satisfies fluorescence excitation's specific demand.
The foregoing has described in detail preferred embodiments of the present invention. It should be understood that numerous modifications and variations can be devised by those skilled in the art in light of the teachings of the present invention without undue experimentation. Therefore, the technical solutions that can be obtained by a person skilled in the art through logic analysis, reasoning or limited experiments based on the prior art according to the concepts of the present invention should be within the scope of protection defined by the claims.
Claims (10)
1. A multi-channel LED light source for fluorescence microscopy imaging, comprising:
four groups of LED channels, which are respectively marked as a first LED channel, a second LED channel, a third LED channel, and a fourth LED channel, wherein the first LED channel and the second LED channel are arranged in parallel, the third LED channel and the fourth LED channel are arranged in parallel, the first LED channel and the third LED channel are arranged orthogonally, and each group of LED channels includes: the LED light emitting device is used for emitting an LED light beam and transmitting the LED light beam through an optical fiber; the condensing lens is arranged opposite to the optical fiber output end of the LED light emitting device and is used for converging and collimating the LED light beam emitted by the LED light emitting device; the optical filter is arranged opposite to the condensing lens and is used for filtering the LED light beam passing through the condensing lens;
the first dichroic mirror is arranged at the intersection of the light paths of the first LED channel and the third LED channel, is obliquely arranged relative to the optical filter of the first LED channel and the optical filter of the third LED channel respectively, and is used for transmitting the LED light beams from the first LED channel and reflecting the LED light beams from the third LED channel and then combining the light beams to emit to the third dichroic mirror;
the second dichroic mirror is arranged at the intersection of the light paths of the second LED channel and the fourth LED channel, is obliquely arranged relative to the optical filter of the second LED channel and the optical filter of the fourth LED channel, and is used for transmitting the LED light beams from the fourth LED channel and reflecting the LED light beams from the second LED channel and then combining the light beams to emit to the third dichroic mirror;
the third dichroic mirror is arranged at the intersection of the light paths of the first LED channel and the fourth LED channel, is obliquely arranged relative to the optical filter of the first LED channel and the optical filter of the fourth LED channel, and is used for transmitting the combined light from the first dichroic mirror and reflecting the combined light from the second dichroic mirror and outputting the combined light.
2. The multi-channel LED light source for fluorescence microscopy according to claim 1, further comprising a light exit port disposed opposite the third dichroic mirror through which the light beam combined by the third dichroic mirror is output.
3. The multi-channel LED light source for fluorescence microscopy according to claim 2,
the four groups of LED channels, the first dichroic mirror, the second dichroic mirror and the third dichroic mirror are arranged in a first chamber;
the multi-channel LED light source for fluorescence microscopy imaging further comprises at least one second chamber, the second chamber is arranged side by side with the first chamber, and the second chamber is provided with the same optical elements and arrangement as the first chamber.
4. The multi-channel LED light source for fluorescence microscopy according to claim 3, further comprising a fourth dichroic mirror obliquely disposed with respect to the light exit port of one of the first and second chambers for transmission of the light beam from the corresponding light exit port.
5. The multi-channel LED light source for fluorescence microscopy according to claim 4, further comprising a reflector obliquely disposed with respect to the light exit ports of the first and second chambers where the fourth dichroic mirror is not disposed, for reflecting and transmitting the light beams from the corresponding light exit ports to the fourth dichroic mirror for further reflection and transmission.
6. The multi-channel LED light source for fluorescence microscopy according to claim 5, further comprising a second condenser lens disposed opposite the fourth dichroic mirror, wherein the light beam transmitted through the fourth dichroic mirror and the light beam reflected through the fourth dichroic mirror are combined and focused by the second condenser lens.
7. The multi-channel LED light source for fluorescence microscopy imaging as defined in claim 6, further comprising a fiber exit, the light beam focused by the second condenser lens being coupled out through a fiber of the fiber exit.
8. The multi-channel LED light source for fluorescence microscopy according to claim 1, wherein each of the LED light emitting devices emits LED light of different wavelength, power and spatial structure.
9. The multi-channel LED light source for fluorescence microscopy according to claim 1, wherein each of the LED light emitting devices is removably or integrally disposed in the corresponding LED channel with the corresponding condenser lens and filter.
10. The multi-channel LED light source for fluorescence microscopy according to claim 1, wherein each of the LED light emitting devices emits continuous or pulsed light.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221007610.0U CN217085406U (en) | 2022-04-27 | 2022-04-27 | Multi-channel LED light source for fluorescence microscopic imaging |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202221007610.0U CN217085406U (en) | 2022-04-27 | 2022-04-27 | Multi-channel LED light source for fluorescence microscopic imaging |
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| CN217085406U true CN217085406U (en) | 2022-07-29 |
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| CN202221007610.0U Active CN217085406U (en) | 2022-04-27 | 2022-04-27 | Multi-channel LED light source for fluorescence microscopic imaging |
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