CN113308367A - Bacteria detection system and bacteria detection method - Google Patents
Bacteria detection system and bacteria detection method Download PDFInfo
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- CN113308367A CN113308367A CN202110763013.4A CN202110763013A CN113308367A CN 113308367 A CN113308367 A CN 113308367A CN 202110763013 A CN202110763013 A CN 202110763013A CN 113308367 A CN113308367 A CN 113308367A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
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Abstract
The invention discloses a bacteria detection system, which comprises a control module, a moving module, a drug sensitive plate fixing device and a detection module, wherein the drug sensitive plate fixing device is used for fixing a drug sensitive plate for storing bacteria to be detected; the detection module comprises a detection device and a shading device, the detection device is arranged above the fixing device of the drug sensitive plate and is used for detecting the luminescence of bacteria in the drug sensitive plate, and the shading device is arranged at the bottom of the detection device and prevents external light from entering the detection device; the moving module is connected with the detection device and drives the detection device to move; the control module is electrically connected with the moving module and the detection device and controls the actions of the detection device and the moving module. Aiming at the defects of the bacteria detection device in the prior art, the light shading device is added, and the interference of an external light source to the detection process is reduced, so that the bacteria detection result of the mycobacterium is more accurate.
Description
Technical Field
The invention relates to the technical field of bacteria detection and diagnosis, in particular to a bacteria detection system and a bacteria detection method.
Background
A large number of bacteria exist in human life, some bacteria are fatal, such as mycobacteria, and the mycobacteria are pathogenic bacteria causing tuberculosis, can invade all organs of the whole body, are most common in pulmonary tuberculosis, and are extremely important for drug sensitivity detection of the mycobacteria.
The mycobacterium tuberculosis (Mtb) grows slowly, and is difficult to detect the propagation and drug resistance of the mycobacterium tuberculosis by genetic operation, the most common drug resistance screening of the mycobacterium tuberculosis at present is to distinguish live bacteria and dead bacteria by utilizing recombinant bacteria of fluorescein plum or green fluorescent protein, and integrate the fluorescein plum plasmid into host bacteria to culture the recombinant bacteria of the self-luminous bacteria. By culturing and screening, drug-resistant self-luminescent mycobacteria and non-luminescent non-drug-resistant mycobacteria can be obtained, thereby judging drug resistance of the drug.
The existing drug sensitivity detection method for mycobacterium tuberculosis is generally manual detection, detection steps are complex, time consumption is long, workload is large, detection results are inaccurate easily due to human reasons, detection personnel have infected risks in the detection process, in addition, the existing detection equipment is poor in shading effect, external light easily interferes the detection results, authenticity of the detection effect cannot be guaranteed, the automation degree of the existing detection equipment is low, and a full-automatic high-flux detection instrument is urgently needed.
Disclosure of Invention
The present invention is directed to a bacteria detection system and a bacteria detection method, which solve the above problems of the prior art.
In order to solve the above-mentioned problems, according to an aspect of the present invention, there is provided a bacteria detecting system, characterized in that it comprises a drug sensitive plate fixing means for fixing a drug sensitive plate for storing bacteria to be detected; the detection module comprises a detection device and a shading device, the detection device is arranged above the medicine sensitive plate fixing device and is used for detecting the luminescence of bacteria in the medicine sensitive plate, and the shading device is arranged below the detection device to prevent external light from entering the detection device; the mobile module is connected with the detection device and drives the detection device to move, and the control module is connected with the mobile module and the detection device and controls the action of the detection device.
In one embodiment, the detection device comprises a shell and a photomultiplier arranged in the shell, wherein a light inlet hole is formed in the shell and is arranged corresponding to the bacteria storage hole in the drug sensitive plate; and the shading device comprises a shading part and a light transmission part, and the light transmission part is matched with the light inlet hole on the shell and transmits the fluorescence of bacteria placed in the drug sensitive plate to the photomultiplier.
In one embodiment, the light shielding device is a light shielding pad fixedly connected to the bottom of the housing, and the light transmission portion is a light transmission hole disposed on the light shielding pad, and the light transmission hole is correspondingly matched with the light inlet hole.
In one embodiment, the bacteria detection system further comprises the drug sensitive plate, a plurality of bacteria storage holes are formed in the drug sensitive plate, the shading device is a shading pad and is fixedly connected to the upper surface of the drug sensitive plate, and a plurality of light transmission holes corresponding to the bacteria storage holes one to one are formed in the shading pad.
In one embodiment, the bacteria detection system further comprises a base, and the drug sensitive plate securing device is movably mounted on the base.
In one embodiment, the control module is connected with the drug sensitive plate fixing device and controls the action of the drug sensitive plate fixing device.
In one embodiment, the bacteria detection system further comprises a housing, the housing and the base cooperate to form a chamber, and the control module, the moving module, the detection module and the drug sensitive plate fixing device are disposed in the chamber.
In one embodiment, the base is provided with a groove which is matched with the shell, the shell is arranged in the groove, and in one embodiment, the groove is arranged around the periphery of the base.
In one embodiment, a sealing member is further disposed in the groove to enhance the tightness between the housing and the base.
In one embodiment, the moving module includes an X-axis moving device, a Y-axis moving device, and a Z-axis moving device to move the detecting device on the X-axis, the Y-axis, and the Z-axis, wherein the X-axis, the Y-axis, and the Z-axis are perpendicular to each other two by two.
In one embodiment, the drug sensitive plate fixing device is a movable rack, and the drug sensitive plate is detachably mounted on the movable rack.
In one embodiment, a bin door is further arranged on the shell, and the movable frame can move out of the bin door when the bin door is opened.
In one embodiment, a display screen is further arranged on the shell, and the display screen is electrically connected with the detection module and the control module and can display the detection result of the detection module.
A bacteria detection method, the bacteria detection system comprises a drug sensitive plate fixing device and a detection module, and the bacteria detection method comprises the following steps:
step one, putting bacteria to be detected into the drug sensitive plate;
secondly, placing the drug sensitive plate on a drug sensitive plate fixing device;
and step three, moving the detection module to the position above the drug sensitive plate and detecting the luminescence of bacteria in the drug sensitive plate, wherein the detection module comprises a detection device and a light shielding device, and the light shielding device is arranged below the detection device to prevent external light from entering the detection device.
Optionally, the bacteria detection system further comprises a moving module, and in step three, the moving module moves the detection module above the drug sensitive plate.
Optionally, the bacteria detection system further comprises a control module, and the control module is connected with the moving module and the detection device and controls the moving module and the detection module to execute the third step.
Optionally, the bacteria detection method is implemented by using a bacteria detection system, a drug sensitive plate fixing device of the bacteria detection system is a movable frame, the movable frame is movably mounted on the base, and the drug sensitive plate is detachably mounted on the movable frame and enters and exits the housing through a bin door to store the drug sensitive plate; the shading device is preferably a shading pad and is fixedly arranged below the detection device; the detection method comprises the following steps:
step one, putting bacteria to be detected into a drug sensitive plate;
moving the movable frame out of the bin gate, and placing the drug sensitive plate on the movable frame;
and step three, the operation control module controls the moving module and drives the detection device to move to the position above the drug sensitive plate, so that the light transmission hole of the shading pad is aligned with the bacteria storage hole of the drug sensitive plate, the moving module continues to drive the detection device to move downwards and compress the shading pad, and light in the bacteria storage hole reaches a seamless shading effect in the process that the drug sensitive plate penetrates through the light transmission hole and enters the detection device, so that the accuracy of bacteria detection is improved.
Aiming at the defects of the detection device in the prior art, the light shading device is added, and the interference of an external light source to the detection process is reduced, so that the mycobacterium bacteria detection result is more accurate.
Drawings
FIG. 1 is a perspective view of a housing of one embodiment of the present invention.
FIG. 2 is a top view of the detection module, control module, drug sensitive plate retainer and base of one embodiment of the present invention assembled.
FIG. 3 is a perspective view of the detection module, the control module, the drug sensitive plate fixing device and the base of the present invention after assembly.
FIG. 4 is a perspective view of a detection module according to one embodiment of the invention.
FIG. 5 is a bottom view of a testing device in accordance with one embodiment of the present invention.
Fig. 6 is a perspective view of a shade device according to an embodiment of the present invention.
FIG. 7 is a perspective view of the engagement of a drug sensitive plate and a light blocking pad according to one embodiment of the present invention.
FIG. 8 is a schematic view of the drug sensitive plate and the light blocking pad of one embodiment of the present invention.
FIG. 9 is a perspective view of a drug sensitive plate and a light blocking pad according to another embodiment of the present invention.
FIG. 10 is a schematic view of another embodiment of the invention showing the configuration of the drug sensitive plate and the light blocking pad.
FIG. 11 is a perspective view of a drug-sensitive plate according to one embodiment of the present invention.
Fig. 12 is a perspective view of a mobile device according to an embodiment of the present invention.
Fig. 13 is a left side view of a shade on a base in accordance with one embodiment of the present invention.
Fig. 14 is a left side view of a shade on a base in accordance with another embodiment of the present invention.
Detailed Description
The preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings so that the objects, features and advantages of the invention can be more clearly understood. It should be understood that the embodiments shown in the drawings are not intended to limit the scope of the present invention, but are merely intended to illustrate the spirit of the technical solution of the present invention.
In the following description, for the purposes of illustrating various disclosed embodiments, certain specific details are set forth in order to provide a thorough understanding of the various disclosed embodiments. One skilled in the relevant art will recognize, however, that the embodiments may be practiced without one or more of the specific details. In other instances, well-known devices, structures and techniques associated with this application may not be shown or described in detail to avoid unnecessarily obscuring the description of the embodiments.
Reference throughout this specification to "one embodiment" or "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment is included in at least one embodiment. Thus, the appearances of the phrases "in one embodiment" or "in an embodiment" in various places throughout this specification are not necessarily all referring to the same embodiment. Furthermore, the particular features, structures, or characteristics may be combined in any suitable manner in one or more embodiments.
In the following description, for the purposes of clearly illustrating the structure and operation of the present invention, directional terms will be used, but terms such as "front", "rear", "left", "right", "outer", "inner", "outer", "inward", "upper", "lower", etc. should be construed as words of convenience and should not be construed as limiting terms.
The invention relates to a bacteria detection system 100, referring to fig. 1-3, the bacteria detection system 100 comprises a control module 11, a moving module 12, a drug sensitive plate fixing device 3 and a detection module 2, wherein the drug sensitive plate fixing device 3 is used for fixing a drug sensitive plate 31 for storing bacteria to be detected; the detection module 2 comprises a detection device 21 and a shading device 22, the detection device 21 is arranged above the drug sensitive plate fixing device 3 and is used for detecting the luminescence of bacteria in the drug sensitive plate 31, and the shading device 22 is arranged at the bottom of the detection device 21 and prevents external light from entering the detection device 21; the moving module 12 is connected with the detection device 21 and drives the detection device 21 to move; the control module 11 is electrically connected to the moving module 12 and the detecting device 21, and controls the operations of the detecting device 21 and the moving module 12.
Preferably, the base 4, the control module 11, the drug sensitive plate holding device 3 and the detection module 2 of the bacteria detection system 100 are fixedly disposed on the base 4 and operate in the receiving space formed by the housing 5 and the base 4.
Further, the bacteria detection system 100 further comprises a housing 5, the housing 5 and the base 4 forming a closed chamber to accommodate the control module 11, the moving module 12, the drug sensitive plate holding device 3 and the detection module 2.
Further, the housing 5 is provided with a door 51, the door 51 is electrically connected to the control module 11 and can be opened automatically, and when the door 51 is opened, the moving frame 31 can move to the outside of the door 51.
The detection device 21 comprises a housing 211 and a photomultiplier 212 disposed within the housing 211, and with reference to fig. 4-5, the housing 211 is of a sealed design as a whole; the housing 211 is provided with a light inlet 2111 corresponding to the light-transmitting portion of the light shielding device 22 and the bacteria storage hole 311 of the drug sensitive plate 31, i.e. light can pass through the light-transmitting portion to the light inlet 2111. It is understood that the shape of the light inlet 2111 is not limited, such as a circle, a square, a polygon, etc., in the embodiment shown in fig. 4-5, the light inlet 2111 is a circle and is disposed at the bottom of the housing 211, and in order to ensure the light shielding effect, the size of the light inlet 2111 must be smaller than the outermost size of the light shielding device 22, i.e., the light inlet 2111 must be covered by the light shielding device 22 to prevent the interference light from entering the housing 211.
The light shielding device 22 of the present invention comprises a light shielding part and a light transmitting part, wherein the light shielding part is used for shielding light from entering the detection device 21 to interfere the detection result, and the light transmitting part is matched with the light inlet hole 211 on the shell 211 and transmits the fluorescence of bacteria placed in the drug sensitive plate 31 to the photomultiplier 212.
Preferably, the light shielding device 22 is a light shielding pad 22, and referring to fig. 6-10, the light shielding portion is a light transmitting hole 221 disposed on the light shielding pad 22, and the light transmitting hole 221 is matched with the light inlet hole 2111; the downward movement of the detection unit 21 presses the light shielding pad 22 to make the light shielding pad 22 and the detection unit 21 and the light shielding pad 22 and the drug sensitive plate 31 of the drug sensitive plate fixing unit 3 closely contact each other to block the interference of external light.
Further, the light shielding pad 22 is fixedly connected to the bottom of the housing 5 of the detection device 21, and integrally covers the light inlet 2111 at the lower end of the housing 211, and the light transmitting hole 221 of the light shielding pad 22 corresponds to the position of the light inlet 2111, referring to fig. 4-7, the light transmitting hole 221 is a cylindrical through hole and is disposed in the middle of the light shielding pad 22. It is to be understood that the shape of the light transmission hole 221 is not limited and may be a cylinder or a rectangular parallelepiped; the size of the light hole 221 is substantially equal to the size of the light inlet 2111, and the size of the light shielding pad 22 is larger than the size of the light inlet 2111, so that the light can be shielded sufficiently and fluorescence in the bacteria storage hole 311 can enter the detection device 21 in a wider range.
Alternatively, the material of the light shielding pad 22 is not limited, and may be silica gel, sponge, fluorine gel, or the like.
Preferably, the shading pad 22 is black to achieve a better shading effect.
Preferably, the blackout pad 22 has a thickness, preferably 3-10 cm.
It will be appreciated that the light shield 22 may be arranged in other ways, in the embodiment shown in fig. 9-10, the light shield 22 is also a light shield 22, in the embodiment shown in fig. 7-8, the light shield 22 is provided with a plurality of light holes 221 and is integrally fixed above the drug sensitive plate 31 of the drug sensitive plate fixing device 3, and the detection device 21 is also positioned above the light shield 22 and can directly pass through the light holes 221 to detect bacteria in the drug sensitive plate 31 of the drug sensitive plate fixing device 3. Specifically, the drug sensitive plate 31 is fixedly arranged on the drug sensitive plate fixing device 3, and a plurality of bacteria storage holes 311 are arranged on the drug sensitive plate 31 to store bacteria to be detected; the shading pad 22 is provided with a plurality of light holes 211, is arranged above the drug sensitive plate 31 and corresponds to the bacteria storage holes 311 one by one, and can be arranged above the drug sensitive plate 31 in a magnetic attraction or adhesion mode; the whole shape of the light shielding pad 22 is basically consistent with that of the drug sensitive plate 31, the number and arrangement of the light holes 221 on the light shielding pad 22 and the bacteria storage holes 311 on the drug sensitive plate 31 are consistent, each light hole 221 corresponds to a bacteria storage hole 311, and the light inlet 2111 of the detection device 21 corresponds to each light hole 221, so that bacteria in each bacteria storage hole 311 can be detected.
Preferably, the shade pad 22 is a solid black material to enhance the shade effect.
Preferably, the distance between the bacteria storage holes 311 of the drug sensitive plate 31 is slightly increased, that is, the distance between the light transmission holes 221 of the light shielding pad 22 is increased, and the interference distance of fluorescence emitted from bacteria in the adjacent bacteria storage holes 311 is correspondingly reduced.
The drug sensitive plate 31 is detachably mounted on the drug sensitive plate fixing device 3, the drug sensitive plate 31 is provided with a plurality of bacteria storage holes, in the embodiment shown in fig. 7-10, the number of the bacteria storage holes is 96, so that 96 bacteria samples can be tested at one time, and high-throughput detection is realized, and it can be understood that the drug sensitive plates 31 with different specifications or the drug sensitive plates 31 with different numbers of bacteria storage holes can be replaced according to requirements.
Preferably, the drug sensitive plate 31 is a black light-shielding material, i.e. each bacteria storage hole 311 is opaque to each other.
The bacteria storage hole 311 corresponds to the light hole 221 of the light shielding pad 22, the bacteria autofluorescence in the bacteria storage hole 311 enters the detection device 21 through the light hole 221 of the light shielding pad 22, and the photomultiplier 212 of the detection device 21 converts the bacteria autofluorescence into an electrical signal to detect the survival status of the bacteria. It will be appreciated that the size of the bacteria storage hole 311 is substantially equal to the size of the light transmissive hole 221 of the light blocking pad 22 and is smaller than the size of the light blocking pad, thereby providing sufficient light blocking and facilitating a greater range of fluorescence light within the bacteria storage hole 311 to enter the detection device 21
Preferably, the drug sensitive plate fixing device 3 is a movable frame 3 and is movably mounted on the base 4, referring to fig. 11, the drug sensitive plate 31 is detachably mounted on the movable frame 3 and enters and exits the housing 211 through the door 51; specifically, the base 4 is provided with a fourth motor 32 and a fourth guide rail 33, the bottom of the movable frame 3 is provided with a fourth slider 34 and a fourth connecting portion, the fourth motor 32 drives a fourth screw 35 to rotate to drive the fourth connecting portion to move along the fourth guide rail 33, the fourth slider 34 is matched with the fourth guide rail 33 to move, the fourth guide rail 33 is arranged along the X axis, the movable frame 32 can be controlled by the fourth motor 32 to remove the bin door 51 along the fourth guide rail 33 so as to install the drug sensitive plate 31, and after the drug sensitive plate 31 is installed, the fourth motor 32 drives the movable frame 32 to move into the housing 5.
Further, the fixing device 3 further comprises a plurality of sensors for detecting the displacement of the movable frame 32, and the sensors are disposed on the base 4.
Further, the control module 11 is connected with the drug sensitive plate fixing device 3 and controls the action of the drug sensitive plate fixing device 3.
The moving module 12 includes an X-axis moving device 121, a Y-axis moving device 122 and a Z-axis moving device 123, the moving module 12 can move the detecting module 2 on the X-axis, the Y-axis and the Z-axis, wherein the X-axis, the Y-axis and the Z-axis are mutually perpendicular to each other in pairs with reference to fig. 12, wherein
The X-axis moving device 121 includes a first motor 1211, a first screw, a first guide rail 1212, a first slider and a first connection portion 1213, the first motor 1211 is fixedly disposed on the base 4 for driving the first screw to rotate, one end of the first connection portion 1213 is fixedly connected to the Y-axis moving device 12, and the other end is connected to the first set of sliders, the first guide rail 1212 is fixedly disposed on the base 4 along the X-axis, the first screw rotates to drive the first set of sliders to move along the first guide rail 1212, that is, the first connection portion 1213 and the Y-axis moving device 12 integrally move along the first guide rail 1212.
The Y-axis moving device 122 includes a second motor 1221, a second screw, a second guide rail 1222, a second slider and a second connecting portion 1223, the second motor 1221 is disposed on the base 4 and can be connected to the second screw, the second motor 1221 can drive the second screw to rotate, one end of the second connecting portion 1223 is connected to the Z-axis moving device 12, and the other end is connected to the second set of sliders, the second guide rail 1222 is fixed on the base 4 along the Y-axis, the second screw rotates to drive the second set of sliders to move along the second guide rail 1222, that is, the second connecting portion 1223 and the Z-axis moving device 12 move along the second guide rail 1222.
The Z-axis moving device 123 includes a third motor 1231, a third screw, a third guide rail 1232, a third slider and a third connecting portion 1233, one end of the third connecting portion 1233 is connected to the detecting module 2, and the other end is connected to the third screw, the third motor 1231 and the third screw are fixedly disposed on the second connecting portion 1223, the third motor 1231 drives the third screw to rotate, the third guide rail 1232 is disposed on the second connecting portion 1223 along the Z-axis, and the third screw rotates to drive the third connecting portion 1233 and the detecting module 2 to move along the Z-axis. Specifically, the detecting device 21 of the detecting module 2 is fixedly connected to the third connecting portion 1233 and is movable in three-dimensional degrees of freedom.
Further, the moving module 12 further includes a plurality of sensors to detect the displacement of the moving module 12, and in particular, one or more sensors are provided corresponding to the X-axis moving device 121, the Y-axis moving device 122, and the Z-axis moving device 123, respectively, to detect the respective displacements.
Alternatively, the shape of the base 4 may be any shape, in the embodiment shown in the figures, the base 4 is a rectangular plate, and the base 4 is provided with a groove 41 matched with the shell 5, that is, the shell 5 can be installed in the groove 41 and matched with the base 4 to form a closed chamber. Preferably, the groove 41 surrounds the periphery of the base 4.
Further, the groove 41 is L-shaped, and referring to fig. 13, the housing 5 can be installed in the groove 41, and the groove 41 can block light of a contact gap between the base 4 and the housing 5;
alternatively, the groove 41 may be a rail type, and referring to fig. 14, light-shielding seals 42 may be disposed in two vertical sides of the groove 41, and the seals 42 may increase the contact tightness between the base 4 and the housing 5.
Preferably, a display screen 52 is further disposed on the housing 5, and the display screen 52 is electrically connected to the control module 11 and the detection module 2 and can display the detection result of the detection module 2.
The control module 11 is connected to the moving module 2 and the detecting device 21 and controls the action of the detecting device 21, and it should be understood that the connection mode of the control module 11 to the moving module or the detecting device 21 includes electrical connection and signal connection.
The invention also relates to a bacteria detection method using the bacteria detection system, the bacteria detection system comprises a drug sensitive plate fixing device and a detection module, and the bacteria detection method comprises the following steps:
step one, putting bacteria to be detected into a drug sensitive plate;
the bacteria to be detected are bacteria which form self-luminescence after being cultivated, and the survival of the bacteria is detected through the luminous intensity of the bacteria to judge the drug resistance of the bacteria through the drug resistance cultivation of the self-luminescence bacteria; a plurality of bacteria storage holes with upward openings are arranged in the drug sensitive plate to store bacteria to be detected, so that the drug sensitive plate is shielded from light.
Secondly, placing a drug sensitive plate on the drug sensitive plate fixing device;
wherein, set up a plurality of block structures on the quick board fixing device of medicine, can detachably fix the quick board of medicine in order to make things convenient for detection module's operation.
And step three, moving the detection module to the position above the drug sensitive plate and detecting the luminescence of bacteria in the drug sensitive plate, wherein the detection module comprises a detection device and a shading device, and the shading device is arranged below the detection device to prevent external light from entering the detection device.
The last light inlet that sets up of detection device, the light shading device set up the light trap corresponding to bacterium storage hole and light inlet, and the hole is stored corresponding to the bacterium in the light trap lower part of quick board of medicine promptly, and the upper end of light trap corresponds detection device's light inlet, and the light in the bacterium storage hole can pass through the light trap and enter detection device with light inlet.
Optionally, the bacteria detection system further comprises a moving module, and in the third step, the moving module moves the detection module in the third step to the position above the drug sensitive plate.
The moving module can move along X, Y and Z axis and is connected with the detection device of the detection module, and the moving module drives the detection device to move so as to realize the detection of bacteria in the bacteria storage holes.
Preferably, the bacteria detection system may further include a control module, which is connected to the moving module and the detection device and controls the moving module and the detection module to perform the third step.
The control module is electrically connected or in signal connection with the moving module and the detection module and controls the operation of the moving module and the detection module.
Preferably, the bacteria detecting system is the bacteria detecting system shown in fig. 1-14, that is, the bacteria detecting method is implemented by using the bacteria detecting system shown in fig. 1-14, the fixing device of the drug sensitive plate of the bacteria detecting system 100 is a movable frame 3, the movable frame 3 is movably mounted on the base 4, the drug sensitive plate 31 is detachably mounted on the movable frame 3 and enters and exits the housing 211 through the door 51 to store the drug sensitive plate 31; the shading device is preferably a shading pad 22 and is fixedly arranged below the detection device 21; the detection method comprises the following steps:
step one, putting bacteria to be detected into a drug sensitive plate 31;
step two, the movable frame 3 is moved out of the bin gate 211, and the drug sensitive plate 31 is placed on the movable frame 3;
step three, the operation control module 11 controls the moving module 12 and drives the detection device 21 to move to the position above the drug sensitive plate 31, so that the light transmission hole 221 of the light shielding pad 22 is aligned with the bacteria storage hole 311 of the drug sensitive plate 31, the moving module 12 continues to drive the detection device 21 to move downwards and compress the light shielding pad 22, and the light in the bacteria storage hole 311 reaches the seamless light shielding effect in the process that the light passes through the light transmission hole 211 from the drug sensitive plate 31 and enters the detection device 21, so that the accuracy of bacteria detection is improved.
While the preferred embodiments of the present invention have been illustrated and described in detail, it should be understood that various changes and modifications of the invention can be effected therein by those skilled in the art after reading the above teachings of the invention. Such equivalents are intended to fall within the scope of the claims appended hereto.
Claims (17)
1. A bacteria detection system, comprising:
the drug sensitive plate fixing device is used for fixing a drug sensitive plate for storing bacteria to be detected;
the detection module comprises a detection device and a shading device, the detection module is arranged above the medicine sensitive plate fixing device and is used for detecting the luminescence of bacteria in the medicine sensitive plate, and the shading device is arranged below the detection device to prevent external light from entering the detection device;
a moving module connected to the detecting device and driving the detecting device to move, an
And the control module is connected with the moving module and the detection device and controls the action of the detection device.
2. The bacteria detection system of claim 1, wherein the detection device comprises a housing and a photomultiplier tube arranged in the housing, the housing is provided with a light inlet, and the light inlet is arranged corresponding to the bacteria storage hole on the drug sensitive plate; and
the shading device comprises a shading part and a light transmission part, wherein the light transmission part is matched with the light inlet hole on the shell and transmits the fluorescence of bacteria placed in the drug sensitive plate to the photomultiplier.
3. The bacteria detection system of claim 2, wherein the light shielding device is a light shielding pad fixedly connected to the bottom of the housing, and the light-transmitting portion is a light-transmitting hole disposed on the light shielding pad, and the light-transmitting hole is correspondingly matched with the light-transmitting hole.
4. The bacteria detection system of claim 2, further comprising the drug sensitive plate, wherein a plurality of bacteria storage holes are formed in the drug sensitive plate, the light shielding device is a light shielding pad and is fixedly connected to the upper surface of the drug sensitive plate, and a plurality of light transmission holes corresponding to the bacteria storage holes one to one are formed in the light shielding pad.
5. The bacteria detection system of claim 1 further comprising a base, wherein said drug sensitive plate securing device is movably mounted to said base.
6. The bacteria detection system of claim 5, wherein the control module is coupled to and controls the operation of the drug sensitive plate fixture.
7. The bacteria detection system of claim 5, further comprising a housing that cooperates with the base to form a chamber, the control module, the movement module, the detection module, and the drug sensitive plate securing device being disposed within the chamber.
8. The bacteria detection system of claim 5 wherein the base is provided with a groove that mates with the housing, the housing being mounted in the groove, in one embodiment the groove is provided around the periphery of the base.
9. The bacteria detection system of claim 8 wherein a seal is further disposed within the recess to enhance the seal between the housing and the base.
10. The bacteria detection system of claim 1, wherein the movement module comprises an X-axis movement device, a Y-axis movement device, and a Z-axis movement device to move the detection device on the X-axis, the Y-axis, and the Z-axis, wherein the X-axis, the Y-axis, and the Z-axis are perpendicular to each other two by two.
11. The bacteria detection system of claim 5 wherein said drug sensitive plate securing means is a mobile frame, said drug sensitive plate being removably mounted to said mobile frame.
12. The bacteria detection system of claim 11 wherein a door is further provided on the housing, the movable rack being movable out of the door when the door is opened.
13. The bacteria detection system of claim 1, wherein a display screen is further disposed on the housing, the display screen being electrically connected to the detection module and the control module and capable of displaying the detection result of the detection module.
14. A bacteria detection method using a bacteria detection system comprising a drug sensitive plate fixture and a detection module, the bacteria detection method comprising the steps of:
step one, putting bacteria to be detected into the drug sensitive plate;
secondly, placing the drug sensitive plate on a drug sensitive plate fixing device;
and step three, moving the detection module to the position above the drug sensitive plate and detecting the luminescence of bacteria in the drug sensitive plate, wherein the detection module comprises a detection device and a light shielding device, and the light shielding device is arranged below the detection device to prevent external light from entering the detection device.
15. The bacteria detection method of claim 14, wherein the bacteria detection system further comprises a moving module, and in the third step, the moving module moves the detection module above the drug sensitive plate.
16. The bacteria detection method of claim 14, wherein the bacteria detection system further comprises a control module, wherein the control module is connected with the moving module and the detection device and controls the moving module and the detection module to execute the third step.
17. The bacteria detection method according to claim 14, wherein the bacteria detection system is the bacteria detection system according to any one of claims 1 to 13.
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| CN202110763013.4A CN113308367A (en) | 2021-07-06 | 2021-07-06 | Bacteria detection system and bacteria detection method |
| PCT/CN2021/106638 WO2023279423A1 (en) | 2021-07-06 | 2021-07-16 | Bacteria detection system and bacteria detection method |
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| CN202110763013.4A CN113308367A (en) | 2021-07-06 | 2021-07-06 | Bacteria detection system and bacteria detection method |
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| WO (1) | WO2023279423A1 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113907820A (en) * | 2021-10-08 | 2022-01-11 | 巢湖市宾雄医疗器械有限公司 | Intelligent suture needle sleeve capable of self-detecting bacteria content and detection method thereof |
| CN113930323A (en) * | 2021-10-25 | 2022-01-14 | 上海泽充生物技术有限公司 | Mycoplasma drug sensitivity fluorescence detection system and detection method |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101130808B (en) * | 2007-07-27 | 2011-08-17 | 南京市胸科医院 | Fast-culturing susceptibility and micro-detection method for mycobacteria and device thereof |
| CN207662789U (en) * | 2017-12-29 | 2018-07-27 | 上海科医联创医学检验所有限公司 | A kind of detector for medicinal allergy |
| CN209307350U (en) * | 2018-12-08 | 2019-08-27 | 安图实验仪器(郑州)有限公司 | Microbial information acquisition device |
| CN212031509U (en) * | 2020-03-30 | 2020-11-27 | 王超强 | Automatic measurement analyzer with wide application range for drug safety detection |
| CN215440453U (en) * | 2021-07-06 | 2022-01-07 | 广州达安基因股份有限公司 | Bacteria detection system |
-
2021
- 2021-07-06 CN CN202110763013.4A patent/CN113308367A/en active Pending
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113907820A (en) * | 2021-10-08 | 2022-01-11 | 巢湖市宾雄医疗器械有限公司 | Intelligent suture needle sleeve capable of self-detecting bacteria content and detection method thereof |
| CN113930323A (en) * | 2021-10-25 | 2022-01-14 | 上海泽充生物技术有限公司 | Mycoplasma drug sensitivity fluorescence detection system and detection method |
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| WO2023279423A1 (en) | 2023-01-12 |
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