CN217997177U - Detection device for nucleic acid extraction and amplification - Google Patents

Abstract

The utility model relates to a detection device for nucleic acid extraction and amplification, which comprises a bearing platform, a sample introduction module, a hole breaking module, a sealing module, a liquid transfer reversing module, a temperature change amplification module, an ultrasonic wall breaking and mixing module and a magnetic bead capturing module; the nucleic acid extraction amplification kit finishes the extraction and amplification of a sampling sample after sequentially passing through a hole breaking module, a liquid transfer reversing module, an ultrasonic wall breaking and uniformly mixing module, a magnetic bead capturing module, a sealing module and a temperature change amplification module through a sample introduction module. The problem that the nucleic acid detection must depend on a P2 laboratory is solved; the full-automatic whole process has no manual intervention, thereby saving precious detection manpower resources; the whole process is totally closed, aerosol pollution is avoided, and the probability of false negative and false positive results is greatly reduced; the thin plate type film is heated, so that the amplification efficiency is greatly improved, and half time can be saved compared with the traditional technology; the traditional temperature change and the emerging constant temperature amplification technology are supported, and the compatibility and the detection flexibility are strong; the integrated level is high and the volume reduces, conveniently carries.

Description

Detection device for nucleic acid extraction and amplification

Technical Field

The utility model belongs to the technical field of bioengineering, especially, relate to a detection device for nucleic acid draws amplification.

Background

PCR (Polymerase Chain Reaction), namely Polymerase Chain Reaction, belongs to one of nucleic acid variable temperature amplification detection technologies, and is also called cell-free molecular cloning and specific DNA sequence in-vitro primer oriented enzymatic amplification technology in some cases; during epidemic situation prevention and control, the national relevant departments definitely put forward the support of 'promoting the production and capacity expansion of the rapid nucleic acid detection equipment with short detection time and convenient operation, increasing policies, and approving the examination and the approval'. The policies of going out of the office are all urging the traditional PCR detection technology to be iteratively upgraded, and products represented by the integrated nucleic acid rapid detection machine gradually become the focus of clinical customers.

A plurality of processes and steps of PCR detection influence the detection result, the difference between different operators is sometimes obvious, and the best measure for reducing personnel interference and environmental interference is to make a full-automatic integrated machine for rapid nucleic acid detection, namely, a sample is directly put on the machine, and the whole detection process is completed by the machine in a full-automatic manner. In short, the integrated nucleic acid amplification and nucleic acid amplification system is integrated, miniaturized and even portable, a sample is input and output in the whole process, and is detected at any moment, and the integrated nucleic acid amplification and nucleic acid amplification system is particularly suitable for conventional sporadic detection scenes in post epidemic situations and large-quantity middle and small hospitals and community clinics without P2 laboratory conditions.

For the nucleic acid detection technology, the integration, automation and miniaturization degree of the analytical instrument and equipment are the main development directions. The current mainstream large and small nucleic acid detection and analysis instrument mainly has the following problems:

1. the nucleic acid extraction technology cannot be absolutely closed, in order to avoid polluting laboratories and instruments, the nucleic acid extraction technology is artificially divided into an extraction instrument and an amplification instrument, and the extraction instrument and the amplification instrument are respectively placed in a P2-level extraction laboratory and an amplification laboratory for nucleic acid detection, so that the investment is huge;

2. although large-scale nucleic acid detection technique high flux multichannel is the machine bulky, occupy valuable laboratory space, in order to realize detecting the automation, need additionally to be equipped with expensive robot arm and detect sample material transmission in drawing and amplification room, small-size nucleic acid detection technique is not big in size, but is the same with large-scale instrument, nucleic acid extraction consumptive material and amplification consumptive material are numerous, the integration is not enough, can't realize automated inspection, need be equipped with the bare-handed operation of full-time measurement personnel.

Disclosure of Invention

The utility model aims to solve the technical problem that a detection device for nucleic acid extraction amplification that the integrated level is high and can realize automatic detection is provided.

The utility model provides a technical scheme that its technical problem adopted is: a detection device for nucleic acid extraction and amplification comprises

A load-bearing platform;

the sample injection module is arranged on the bearing platform and is used for conveying the nucleic acid extraction amplification kit into the detection device;

the hole breaking module is arranged beside the sample injection module and is used for carrying out hole breaking operation on the extraction part of the nucleic acid extraction amplification kit;

the liquid transferring reversing module is arranged beside the sample introduction module and is used for carrying out sample processing operation on an extraction part of the nucleic acid extraction amplification kit;

the ultrasonic wall breaking and mixing module is arranged beside the sample injection module and is used for carrying out sample extraction operation on the sample treated in the extraction part;

the magnetic bead capturing module is arranged beside the sample introduction module and is used for capturing nucleic acid in a sample;

the sealing module is arranged beside the sample introduction module and is used for sealing the amplification part of the nucleic acid extraction and amplification kit;

the temperature-variation amplification module is arranged beside the sample introduction module and is used for carrying out temperature-rise amplification operation on an amplification part of the nucleic acid extraction amplification kit;

the nucleic acid extraction amplification kit finishes extraction and amplification of a sampling sample after sequentially passing through a hole breaking module, a liquid transfer reversing module, an ultrasonic wall breaking and uniformly mixing module, a magnetic bead capturing module, a sealing module and a temperature change amplification module through a sample introduction module.

Further specifically, advance the appearance module including be fixed in the load-bearing platform on advance a kind slide rail, set up advance a kind slider on advancing a kind slide rail, set up advance a kind tool board on advancing a kind slider, set up advance a kind motor on load-bearing platform and set up the belt group of advancing a kind on load-bearing platform, advance a kind tool board and be fixed in advance a kind belt group is last, nucleic acid draws the amplification kit place in advance on the kind tool board.

Further specifically, be fixed with the kind locating plate on the appearance tool board of advancing be provided with a plurality of kind sensors on the load-bearing platform, advance the kind locating plate and move to appointing advance kind sensor department, advance kind motor stop motion.

The hole breaking module comprises a hole breaking support arranged on the bearing platform, a hole breaking screw rod arranged on the hole breaking support, a hole breaking screw nut arranged on the hole breaking screw rod, a hole breaking block arranged on the hole breaking screw nut and a hole breaking motor driving the hole breaking screw rod to rotate, wherein a plurality of hole breaking pointed cones are arranged on the hole breaking block; and a guide rail sliding block set is arranged between the hole breaking support and the hole breaking block.

The sealing module comprises a sealing support arranged on the bearing platform, a sealing screw rod arranged on the sealing support, a sealing screw rod nut arranged on the sealing screw rod, a sealing block arranged on the sealing screw rod nut and a sealing motor driving the sealing screw rod to rotate, a sealing probe is arranged on the sealing support, a sealing driving part is arranged on the sealing block, the sealing driving part pushes the sealing probe to approach the nucleic acid extraction amplification kit, and a reset piece is arranged between the sealing probe and the sealing support; and a guide rail sliding block set is arranged between the sealing support and the sealing block.

Further specifically, move liquid switching-over module including set up move liquid switching-over electric jar on load-bearing platform, by move liquid switching-over electric jar driven move liquid lifter plate, set up in move liquid rotating electrical machines on the lifter plate, by rotating electrical machines driven rotary driving piece and plunger, plunger and rotary driving piece all insert in the nucleic acid extraction amplification reagent box.

Further specifically, the rotary driving part is a cylinder, the plunger is located at the center of the rotary driving part, and a driving notch is formed in the rotary driving part.

Further specifically, the temperature-dependent amplification module comprises a temperature-guiding part arranged on the bearing platform, and a heating and refrigerating unit arranged on the temperature-guiding part, wherein the heating and refrigerating unit is used for rapidly heating or cooling the amplification part of the nucleic acid extraction and amplification kit.

Further specifically, the temperature conducting part comprises a first plate and a second plate which are arranged in parallel, the end part of the first plate close to the bearing platform is connected with the end part of the second plate close to the bearing platform through a third plate, the first plate, the second plate and the third plate enclose an amplification chamber, the amplification part can enter the amplification chamber, and the first plate is attached to the heating and refrigerating unit.

Further specifically, the ultrasonic wall-breaking blending module comprises a blending bracket arranged on the bearing platform, a blending screw rod arranged on the blending bracket, a blending screw nut arranged on the blending screw rod, a blending block arranged on the blending screw nut, an ultrasonic blending probe arranged on the blending block and a blending motor arranged on the blending bracket, wherein the ultrasonic probe has elasticity and can be tightly pressed at the bottom of the kit; the ultrasonic uniformly-mixing probe is close to or far from the nucleic acid extraction and amplification kit through lifting.

Further specifically, the magnetic bead capturing module comprises a capturing support arranged on the bearing platform, a shielding sleeve arranged on the capturing support, and a magnetic capturing head arranged in the shielding sleeve, wherein the shielding sleeve faces an opening on one side of the kit, and the magnetic capturing head is positioned beside the nucleic acid extraction and amplification kit.

Further specifically, a reading module is arranged beside the temperature-change amplification module, the reading module comprises a reading support arranged on the bearing platform, a reading electric cylinder arranged on the reading support, a reading head fixing block driven by the reading electric cylinder and at least one reading head arranged on the reading head fixing block, and the reading head receives an optical signal of the amplification part.

The beneficial effects of the utility model are that: the problem that nucleic acid detection must depend on a P2 laboratory is solved, and the development of nucleic acid detection analysis becomes possible under the conditions of a conventional laboratory and various portable detection (poct) scenes; the full-automatic whole process has no manual intervention, thereby saving precious detection manpower resources, particularly in the epidemic situation period; the whole process is totally closed, aerosol pollution is avoided, and the probability of false negative and false positive results is greatly reduced; the thin plate type film is heated, so that the amplification efficiency is greatly improved, and half time can be saved compared with the traditional technology; the traditional temperature change and the emerging constant temperature amplification technology are supported, and the compatibility and the detection flexibility are strong; the integrated level is high, and the volume reduces greatly, conveniently carries.

Drawings

FIG. 1 is a schematic structural view (with a case) of the detection apparatus for nucleic acid extraction and amplification of the present invention;

FIGS. 2 and 3 are schematic structural views (without a housing) of the detection device for nucleic acid extraction and amplification of the present invention;

FIG. 4 is a schematic structural diagram of the sample injection module of the present invention;

fig. 5 is a schematic structural diagram of the hole breaking module of the present invention;

fig. 6 is a schematic structural diagram of the liquid-transferring reversing module of the present invention;

fig. 7 is a schematic structural diagram of the ultrasonic wall-breaking and mixing module of the present invention;

FIG. 8 is a schematic structural diagram of a magnetic bead capture module according to the present invention;

fig. 9 and 10 are schematic structural views of the sealing module of the present invention;

FIG. 11 is a schematic structural diagram of a temperature-dependent amplification module according to the present invention;

FIG. 12 is a schematic structural view of the thermal conductor of the present invention;

fig. 13 is a schematic structural diagram of the reading module of the present invention.

In the figure: 10. nucleic acid extraction and amplification kit; 11. a high efficiency filter; 12. a power source; 13. a housing; 14. an ultraviolet lamp; 15. sweeping the wharf; 16. a door lock mechanism; 20. a load-bearing platform;

30. a sample introduction module; 31. a sample injection slide rail; 32. a sample introduction slide block; 33. a sample injection jig plate; 34. a sample injection motor; 35. a first fixing plate; 36. a second fixing plate; 37. a sample introduction belt; 38. a sample injection positioning plate; 39. a sample introduction sensor;

40. a hole breaking module; 41. a hole breaking bracket; 42. a hole breaking screw rod; 43. breaking a hole screw nut; 44. breaking the hole block; 45. a hole breaking motor; 46. breaking a hole pointed cone; 47. breaking a hole guide rail; 48. a hole breaking sliding block;

50. a pipetting reversing module; 51. liquid transfer reversing electric cylinder; 52. a pipetting lifting plate; 53. a rotating electric machine; 54. a rotary drive member; 55. a plunger;

60. an ultrasonic wall breaking and uniformly mixing module; 61. uniformly mixing the bracket; 62. uniformly mixing the lead screw; 63. mixing a screw nut; 64. uniformly mixing blocks; 65. an ultrasonic mixing probe; 66. a mixing motor;

70. a magnetic bead capture module; 71. a capturing scaffold; 72. a magnetic capture head;

80. a sealing module; 81. a sealing bracket; 82. a sealing screw rod; 83. a sealing screw rod nut; 84. a sealing block; 85. a sealing motor; 86. sealing the probe; 87. a sealing drive section; 88. a reset member; 89. a first wedge-shaped face; 810. a second wedge-shaped face;

90. a temperature-dependent amplification module; 91. a temperature conduction member; 92. a heat sink; 93. a silica gel pad; 94. a thermoelectric chip; 911. a first plate; 912. a second plate; 913. a third plate; 914. an amplification chamber; 915. a reading through hole;

100. a reading module; 101. a reading support; 102. reading an electric cylinder; 103. a reading head fixing block; 104. a reading head.

Detailed Description

The technical solution of the present invention will be described clearly and completely with reference to the accompanying drawings, and obviously, the described embodiments are some, but not all embodiments of the present invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts all belong to the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art. Furthermore, the technical features mentioned in the different embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.

The utility model relates to a detection device for nucleic acid extraction amplifys is to the structural design that an integral type draws the kit to go on, the integral type draws the kit to be the following nucleic acid extraction amplifys kit 10, nucleic acid extraction amplifys kit 10 includes extraction portion and amplification portion, is provided with sample chamber, stock solution chamber, transfer chamber and mixed reaction chamber in the extraction portion, set up the PCR reaction chamber in the amplification portion, can realize the full automatization of extraction, amplification and reading through this detection device.

As shown in FIGS. 1 to 3, a detection device for nucleic acid extraction and amplification comprises

The bearing platform 20 is used for fixing the related functional modules and ensuring the normal operation of each functional module;

the sample injection module 30 is arranged on the bearing platform 20 and is used for conveying the nucleic acid extraction amplification kit 10 into the detection device;

the hole breaking module 40 is arranged beside the sample injection module 30 and is used for carrying out hole breaking operation on the nucleic acid extraction and amplification kit 10;

a liquid-transfering reversing module 50, which is arranged beside the sample-feeding module 30 and is used for carrying out sample processing operation on the extraction part of the nucleic acid extraction amplification kit 10;

an ultrasonic wall breaking and mixing module 60 arranged beside the sample injection module 30 and used for carrying out sample extraction operation on the samples processed in the extraction part;

a magnetic bead capturing module 70 disposed beside the sample introduction module 30 for capturing nucleic acid in the sample;

a sealing module 80, disposed beside the sample injection module 30, for sealing the amplification part of the nucleic acid extraction and amplification kit 10;

the temperature-dependent amplification module 90 is arranged beside the sample introduction module 30 and is used for carrying out temperature-dependent amplification operation on the amplification part of the nucleic acid extraction and amplification kit 10;

firstly, the nucleic acid extraction amplification kit 10 enters the detection device through the sample injection module 30, the sample injection module 30 transports the kit to the side of the hole breaking module 40 to stop, the hole breaking module 40 performs hole breaking operation on the top of the kit, and the main purpose of the kit is to ensure that external gas can enter the liquid storage cavity to enable treatment liquid in the liquid storage cavity to be smoothly transferred; then, the sample introduction module 30 transports the reagent kit to the liquid transfer reversing module 50, the liquid in the sample cavity and the liquid storage cavity is sequentially transferred into the mixing reaction cavity through the liquid transfer reversing module 50, meanwhile, the liquid is subjected to ultrasonic wall breaking and uniformly mixing through the ultrasonic wall breaking and uniformly mixing module 60 outside the mixing reaction cavity, then nucleic acid molecules in the sample are captured through the magnetic bead capturing module 70, then the nucleic acid molecules obtained by the magnetic bead capturing module are separated from the magnetic bead capturing module 70 through eluent in the liquid storage cavity, and then the separated nucleic acid molecules are sent to an amplification part through the liquid transfer reversing module 50; then, the reagent kit is transported to the sealing module 80 through the sample injection module 30, and the amplification part is subjected to ultrasonic sealing operation through the sealing module 80; then, the reagent kit is transported to the temperature-varying amplification module 90 through the sample injection module 30, and the amplification part is subjected to rapid temperature-increasing or temperature-decreasing amplification operation at the temperature-varying amplification module 90 until the amplification is completed.

The carrying platform 20 is a rectangular plate, the exterior of the detection device is provided with a shell 13, the carrying platform 20 divides a cavity in the shell 13 into an upper cavity above the carrying platform 20 and a lower cavity below the carrying platform 20, and the shell 13 is provided with a sample inlet which is arranged at an inlet of the sample module 30 so as to facilitate the installation of the kit on the sample module 30; a door panel is provided on the housing 13 which can be opened to allow for the management and maintenance of internal components but which is not allowed to be opened when the inspection device is in operation, so a door locking mechanism 16 is provided between the load-bearing platform 20 and the door panel to ensure that the inspection device cannot be opened when in operation.

The sample injection module 30 is disposed in the upper chamber, and as shown in fig. 4, the sample injection module 30 includes a sample injection slide rail 31 fixed on the bearing platform 20, a sample injection slide block 32 disposed on the sample injection slide rail 31, a sample injection jig plate 33 disposed on the sample injection slide block 32, a sample injection motor 34 disposed on the bearing platform 20, and a sample injection belt set disposed on the bearing platform 20; the sample injection belt set comprises a first fixing plate 35 and a second fixing plate 36 which are fixed on the bearing platform 20, the first fixing plate 35 is located on one side of the bearing platform 20, the second fixing plate 36 is located on the other side of the bearing platform 20, the sample injection motor 34 is arranged on the first fixing plate 35, a first belt pulley is arranged at the output end of the sample injection motor 34, a second belt pulley is arranged on the second fixing plate 36, a sample injection belt 37 is wound between the first belt pulley and the second belt pulley, the sample injection jig plate 33 is fixed on the sample injection belt 37, and the nucleic acid extraction amplification kit 10 is placed on the sample injection jig plate 33; the sample feeding motor 34 drives the sample feeding belt 37 to move, and the sample feeding belt 37 drives the sample feeding jig plate 33 and the reagent kit to move along the direction of the sample feeding slide rail 31.

Because the sample injection module 30 needs to stay at each functional module for corresponding operation in the moving process, in order to ensure the accurate position thereof, a sample injection positioning plate 38 is fixed on the sample injection jig plate 33, a plurality of sample injection sensors 39 are arranged on the bearing platform 20, the sample injection positioning plate 38 moves to the position of the specified sample injection sensor 39, and the sample injection motor 34 stops moving; a sample sensor 39 is arranged at the sample inlet of the shell 13, and the sample sensor 39 can be arranged at the hole breaking module 40, the sealing module 80, the liquid transfer reversing module 50, the temperature change amplification module 90, the ultrasonic wall breaking and uniformly mixing module 60 and the magnetic bead capturing module 70; and when different functional modules can operate at same station, can set up a sample sensor 39 in this station department, promptly in this scheme, move liquid switching-over module 50, temperature change amplification module 90, ultrasonic wave broken wall mixing module 60 and magnetic bead and catch module 70 and all can operate at same station, here set up a sample sensor 39 can.

The hole breaking module 40 is disposed in the upper chamber, as shown in fig. 5, the hole breaking module 40 includes a hole breaking support 41 disposed on the bearing platform 20, a hole breaking screw rod 42 disposed on the hole breaking support 41, a hole breaking screw nut 43 disposed on the hole breaking screw rod 42, a hole breaking block 44 disposed on the hole breaking screw nut 43, and a hole breaking motor 45 driving the hole breaking screw rod 42 to rotate, a plurality of hole breaking pointed cones 46 are disposed on the hole breaking block 44, and the number of the hole breaking pointed cones 46 is the same as the number of the liquid storage chambers; meanwhile, a guide rail sliding block set is arranged between the hole breaking bracket 41 and the hole breaking block 44, so that the hole breaking module 40 can move smoothly without shaking, and the piercing precision is improved; the guide rail sliding block group consists of a hole breaking guide rail 47 fixed on the hole breaking bracket 41 and a hole breaking sliding block 48 arranged on the hole breaking guide rail 47; when the reagent kit moves to the position of the hole breaking module 40, the hole breaking motor 45 moves to drive the hole breaking screw rod 42 to rotate, so as to drive the hole breaking block 44 to move downwards, the hole breaking pointed cone 46 punctures the shell above the liquid storage cavity, external air can be ensured to enter the liquid storage cavity, then the hole breaking motor 45 drives the hole breaking block 44 to move upwards to leave the reagent kit, and then the sample injection module 30 transports the reagent kit to the liquid transfer reversing module 50 to perform the next process.

The pipetting reversing module 50 is disposed in the upper chamber, as shown in fig. 6, the pipetting reversing module 50 includes a pipetting reversing electric cylinder 51 disposed on the carrying platform 20, a pipetting lifting plate 52 driven by the pipetting reversing electric cylinder 51, a rotating motor 53 disposed on the pipetting lifting plate 52, a rotating drive member 54 driven by the rotating motor 53, and a plunger 55, wherein the plunger 55 and the rotating drive member 54 are inserted into the nucleic acid extraction amplification kit 10; the rotary driving part 54 is cylindrical, two driving notches are arranged on the rotary driving part 54, and the two driving notches are oppositely arranged on the cylinder wall; the plunger 55 is arranged at the central position of the rotary driving part 54, meanwhile, a gap is ensured to be arranged between the plunger 55 and the rotary driving part 54, the plunger 55 is inserted into a piston in the transition cavity, and the rotary driving part 54 is inserted into the outer wall of the transition cavity and can drive the transition cavity to rotate; meanwhile, the sample injection module 30 can realize the linear motion of the transition cavity in the reagent box, namely the positions of the plunger 55 and the rotary driving piece 54 are kept unchanged, and the sample injection jig plate 33 moves; the transfer switching cylinder 51 moves up and down the plunger 55 and the rotary drive member 54, the plunger 55 moves up and down to suck and discharge the treatment liquid into and from the transition chamber, and the rotary motor 53 rotates the rotary drive member 54.

While the pipetting reversing module 50 works, the ultrasonic wall-breaking blending module 60 also works, and as shown in fig. 7, the ultrasonic wall-breaking blending module 60 comprises a blending bracket 61 arranged on the bearing platform 20, a blending screw rod 62 arranged on the blending bracket 61, a blending screw rod nut 63 arranged on the blending screw rod 62, a blending block 64 arranged on the blending screw rod nut 63, an ultrasonic blending probe 65 arranged on the blending block 64, and a blending motor 66 arranged on the blending bracket 61; compression spring is established to the cover on ultrasonic mixing probe 65, and ultrasonic probe 65 can closely press close to kit and reinforcing ultrasonic effect through compression spring, ultrasonic broken wall mixing module 60 sets up in the cavity of resorption be provided with the through hole on load-bearing platform 20, mixing motor 66 makes ultrasonic mixing probe 65 stretches into to the cavity of resorption from the cavity of resorption in from the through hole, ultrasonic mixing probe 65 is arranged in mixed reaction chamber bottom and is handled the sample of mixed reaction intracavity through the ultrasonic wave.

The magnetic bead capturing module 70 is disposed in the upper chamber, as shown in fig. 8, the magnetic bead capturing module 70 includes a capturing bracket 71 disposed on the carrying platform 20, a shielding sleeve disposed on the capturing bracket 71, and a capturing head magnetic capturing head 72 disposed in the shielding sleeve on the capturing bracket 71, the shielding sleeve has an opening facing one side of the mixing reaction chamber, and the capturing head magnetic capturing head 72 is located beside the nucleic acid extraction and amplification kit 10. The shielding sleeve is made of an aluminum alloy material, so that other magnetism leakage which is not opposite to the direction of the mixing reaction cavity is avoided, and other ferrous metal parts are easily magnetized by the magnetism leakage, so that the magnetic bead capturing function is disordered; when the magnetic beads need to be captured, the magnetic capture head 72 captures the magnetic beads in the mixing reaction cavity to a specified position, then the waste liquid is discharged through the transition cavity, and the magnetic beads are left in the mixing reaction cavity; after the sample is processed, the nucleic acid molecules are sent to the amplification part through the transition cavity, and then the reagent kit is transported to the sealing module 80 through the sample injection module 30 for sealing operation.

The sealing module 80 is disposed in the upper chamber, as shown in fig. 9 and 10, the sealing module 80 includes a sealing support 81 disposed on the bearing platform 20, a sealing screw 82 disposed on the sealing support 81, a sealing screw nut 83 disposed on the sealing screw 82, a sealing block 84 disposed on the sealing screw nut 83, and a sealing motor 85 for driving the sealing screw 82 to rotate, a sealing probe 86 is disposed on the sealing support 81, a sealing driving portion 87 is disposed on the sealing block 84, the sealing driving portion 87 pushes the sealing probe 86 to move toward an amplification portion of the nucleic acid extraction amplification kit 10 and perform a sealing operation on the amplification portion, and meanwhile, a guide rail slider group is disposed between the sealing support 81 and the sealing block 84 to ensure the stability of the operation; a reset piece 88 is arranged between the sealing probe 86 and the sealing bracket 81, and the reset piece 88 can be a compression spring; when the sealing driving part 87 leaves the sealing probe 86, the reset piece 88 makes the sealing probe 86 reset to the initial position; the sealing driving part 87 is provided with a first wedge-shaped surface 89, the sealing probe 86 is fixed on a probe fixing block, the probe fixing block is provided with a second wedge-shaped surface 810, and the first wedge-shaped surface 89 is matched with the second wedge-shaped surface 810, so that the vertical movement of the sealing driving part 87 can be changed into the horizontal movement of the sealing probe 86, and the sealing of the amplification part is facilitated; after sealing is completed, the sample injection module 30 transports the kit to the temperature-dependent amplification module 90 for processing the amplification part.

The temperature-dependent amplification module 90 is disposed in the upper chamber, as shown in fig. 11, the temperature-dependent amplification module 90 includes a temperature conducting member 91 disposed on the carrying platform 20, and a heating and cooling unit disposed on the temperature conducting member 91, as shown in fig. 12, the temperature conducting member 91 includes a first plate 911 and a second plate 912 disposed in parallel, an end portion of the first plate 911 close to the carrying platform 20 is connected to an end portion of the second plate 912 close to the carrying platform 20 through a third plate 913, and the first plate 911, the second plate 912 and the third plate 913 enclose an amplification chamber 914; the heating and refrigerating unit comprises a radiator 92 fixed on the bearing platform 20, a silica gel pad 93 arranged on the radiator 92 and a thermoelectric chip 94 arranged in the silica gel pad 93, wherein the silica gel pad 93 and the thermoelectric chip 94 are attached to the first plate 911, and the thermoelectric chip is positively or reversely connected to realize the heating or refrigerating effect; the amplification part can enter the amplification chamber 914, the thermoelectric chip 94 is heated, heat is transmitted to the heat conducting piece 91 to realize temperature rise amplification of the amplification part, the silica gel pad 93 and the radiator 92 are matched to quickly transfer the heat to the external environment, the purpose is to conveniently control the temperature in the amplification chamber 914, meanwhile, a temperature sensor can be arranged in the amplification chamber 914, and the operation of the thermoelectric chip 94 and the radiator 92 is controlled through the feedback of the temperature sensor to accurately control the temperature.

Further, in order to facilitate direct reading of the amplified sample, a reading module 100 is disposed beside the temperature-dependent amplification module 90, the reading module 100 is disposed in the lower chamber, as shown in fig. 13, the reading module 100 includes a reading support 101 disposed on the carrying platform 20, a reading electric cylinder 102 disposed on the reading support 101, a reading head fixing block 103 driven by the reading electric cylinder 102, and at least one reading head 104 disposed on the reading head fixing block 103, and the reading head 104 receives an optical signal of the amplification part; in order to ensure the light signal to be emitted from the amplification chamber 914 in the temperature guide 91, a plurality of reading through holes 915 are provided on the third plate 913, the number of the reading through holes 915 is the same as the number of the PCR reaction chambers in the amplification part, and the light signal generated by the amplification part is emitted through the reading through holes 915 and is read by the reading head 104; in the scheme, the number of the reading heads is set to be 4, and the number of the reading heads can be specifically set according to needs.

The data read by the reading head 104 can be directly displayed through a display screen arranged on the shell 13 after being processed, so that an operator can conveniently and quickly obtain the required data; meanwhile, in order to ensure that the whole testing device is not polluted by germs, an ultraviolet lamp 14 for disinfection and sterilization is arranged at the top of an upper chamber in the shell 13, and after one nucleic acid test is finished, the ultraviolet lamp 14 is required to be used for sterilization, and then the next nucleic acid test is carried out; the shell 13 is provided with windows for communicating the inside of the shell 13 with the external environment and keeping the internal and external air pressures balanced, and in order to ensure that the inside of the shell 13 is not polluted, the windows are provided with high-efficiency filters 11 and assisted with fans to rapidly exchange the internal air with the external air; a power supply module 12 is arranged in the lower chamber, and the power supply module 12 provides energy for driving the test device to operate; the power module 12 may be a plug directly connected to the mains supply, or may be a dry battery or a rechargeable lithium battery, etc., and when the battery is used for supplying power, the power module is suitable for any working condition and is convenient to carry.

Meanwhile, a control module is arranged in the lower chamber, the control module can control a motor and an electric cylinder in the testing device, can receive signals sent by various sensors and signals received by the reading head 104, and outputs control signals or readable signals after the signals are processed by an MCU in the control module.

Based on above-mentioned structure, in order to can quick identification bar code or two-dimensional code on the kit, as shown in fig. 1 be provided with on the bearing platform 20 by the introduction port and sweep pier 15, when the kit from the introduction port assembly to advance a kind tool board 33, scanning head 15 sweeps the sign indicating number and discerns bar code or two-dimensional code on the kit, acquires corresponding user information.

In conclusion, the nucleic acid extraction amplification kit 10 is applied to the test device, and the kit is sent to the interior of the test device through the sample introduction module 30, so that the functions of automatic hole breaking, automatic extraction, automatic amplification and automatic reading can be realized, the functions of direct amplification and direct reading of a sample are realized, manual intervention is not needed in an intermediate process, the possibility of sample pollution can be reduced, and the accuracy of sample test can be improved; the detection device is in a closed state in the process of nucleic acid detection, and meanwhile, the detection device realizes unmanned intervention, and has small volume, high integration level and automatic detection effect.

It is to be emphasized that: the above embodiments are only preferred embodiments of the present invention, and are not intended to limit the present invention in any form, and any simple modifications, equivalent changes and modifications made by the technical spirit of the present invention to the above embodiments are all within the scope of the technical solution of the present invention.

Claims (12)

1. A detection device for nucleic acid extraction and amplification is characterized by comprising

A load-bearing platform (20);

the sample introduction module (30) is arranged on the bearing platform (20) and is used for conveying the nucleic acid extraction amplification kit (10) into the detection device;

the hole breaking module (40) is arranged beside the sample introduction module (30) and is used for carrying out hole breaking operation on an extraction part of the nucleic acid extraction and amplification kit (10);

the liquid transfer reversing module (50) is arranged beside the sample introduction module (30) and is used for carrying out sample treatment operation on an extraction part of the nucleic acid extraction and amplification kit (10);

the ultrasonic wall-breaking and uniformly-mixing module (60) is arranged beside the sample injection module (30) and is used for carrying out sample extraction operation on the sample treated in the extraction part of the nucleic acid extraction and amplification kit (10);

the magnetic bead capture module (70) is arranged beside the sample introduction module (30) and is used for capturing nucleic acid in the sample;

the sealing module (80) is arranged beside the sample injection module (30) and is used for sealing the amplification part of the nucleic acid extraction and amplification kit (10);

the temperature-dependent amplification module (90) is arranged beside the sample injection module (30) and is used for carrying out temperature-dependent amplification operation on an amplification part of the nucleic acid extraction and amplification kit (10);

the nucleic acid extraction amplification kit (10) finishes extraction and amplification of a sampling sample after sequentially passing through a hole breaking module (40), a liquid transfer reversing module (50), an ultrasonic wall breaking and uniformly mixing module (60), a magnetic bead capturing module (70), a sealing module (80) and a temperature change amplification module (90) through a sample injection module (30).

2. The device for detecting nucleic acid extraction and amplification according to claim 1, wherein the sample injection module (30) comprises a sample injection slide rail (31) fixed on the supporting platform (20), a sample injection slide block (32) disposed on the sample injection slide rail (31), a sample injection jig plate (33) disposed on the sample injection slide block (32), a sample injection motor (34) disposed on the supporting platform (20), and a sample injection belt set disposed on the supporting platform (20), wherein the sample injection jig plate (33) is fixed on the sample injection belt set, and the nucleic acid extraction and amplification kit (10) is disposed on the sample injection jig plate (33).

3. The device for detecting nucleic acid extraction and amplification according to claim 2, wherein a sample positioning plate (38) is fixed on the sample fixture plate (33), a plurality of sample sensors (39) are disposed on the supporting platform (20), the sample positioning plate (38) moves to the position where the sample sensors (39) are designated, and the sample motor (34) stops moving.

4. The detection device for nucleic acid extraction and amplification according to claim 1, wherein the hole breaking module (40) comprises a hole breaking support (41) arranged on the bearing platform (20), a hole breaking screw rod (42) arranged on the hole breaking support (41), a hole breaking screw rod nut (43) arranged on the hole breaking screw rod (42), a hole breaking block (44) arranged on the hole breaking screw rod nut (43), and a hole breaking motor (45) for driving the hole breaking screw rod (42) to rotate, wherein a plurality of hole breaking pointed cones (46) are arranged on the hole breaking block (44); and a guide rail sliding block set is arranged between the hole breaking support (41) and the hole breaking block (44).

5. The detection apparatus for nucleic acid extraction and amplification according to claim 1, wherein the sealing module (80) includes a sealing support (81) disposed on the bearing platform (20), a sealing screw rod (82) disposed on the sealing support (81), a sealing screw rod nut (83) disposed on the sealing screw rod (82), a sealing block (84) disposed on the sealing screw rod nut (83), and a sealing motor (85) for driving the sealing screw rod (82) to rotate, a sealing probe (86) is disposed on the sealing support (81), a sealing driving portion (87) is disposed on the sealing block (84), the sealing driving portion (87) pushes the sealing probe (86) to approach the nucleic acid extraction and amplification kit (10), and a reset piece (88) is disposed between the sealing probe (86) and the sealing support (81); a guide rail sliding block set is arranged between the sealing support (81) and the sealing block (84).

6. The detection apparatus for nucleic acid extraction and amplification according to claim 1, wherein the pipetting reversing module (50) comprises a pipetting reversing electric cylinder (51) arranged on the carrying platform (20), a pipetting lifting plate (52) driven by the pipetting reversing electric cylinder (51), a rotating motor (53) arranged on the pipetting lifting plate (52), a rotating driving member (54) driven by the rotating motor (53), and a plunger (55), wherein the plunger (55) and the rotating driving member (54) are inserted into the nucleic acid extraction and amplification kit (10).

7. The detection apparatus for nucleic acid extraction and amplification according to claim 6, wherein the rotary drive member (54) is cylindrical, the plunger (55) is located at the center of the rotary drive member (54), and the rotary drive member (54) is provided with a driving notch.

8. The detection apparatus for nucleic acid extraction and amplification according to claim 1, wherein the temperature-dependent amplification module (90) includes a temperature-guiding element (91) disposed on the carrying platform (20), and a heating and cooling set disposed on the temperature-guiding element (91), and the amplification unit of the nucleic acid extraction and amplification kit (10) is rapidly heated or cooled for amplification by the heating and cooling set.

9. The detection apparatus for nucleic acid extraction and amplification according to claim 8, wherein the thermal conductor (91) comprises a first plate (911) and a second plate (912) arranged in parallel, the end of the first plate (911) near the platform (20) and the end of the second plate (912) near the platform (20) are connected by a third plate (913), the first plate (911), the second plate (912) and the third plate (913) enclose an amplification chamber (914), the amplification part can enter the amplification chamber (914), and the first plate (911) is attached to the heating and cooling unit.

10. The detection device for nucleic acid extraction and amplification according to claim 1, wherein the ultrasonic wall-breaking and mixing module (60) comprises a mixing support (61) arranged on the bearing platform (20), a mixing screw rod (62) arranged on the mixing support (61), a mixing screw nut (63) arranged on the mixing screw rod (62), a mixing block (64) arranged on the mixing screw nut (63), an ultrasonic mixing probe (65) arranged on the mixing block (64), and a mixing motor (66) arranged on the mixing support (61), wherein the ultrasonic mixing probe (65) has elasticity and can be tightly pressed at the bottom of the kit; the ultrasonic uniform mixing probe (65) is close to or far away from the nucleic acid extraction amplification kit (10) through lifting.

11. The detection apparatus for nucleic acid extraction and amplification as claimed in claim 1, wherein the magnetic bead capture module (70) includes a capture support (71) disposed on the carrying platform (20), a shielding sleeve disposed on the capture support (71), and a magnetic capture head (72) disposed in the shielding sleeve, the shielding sleeve is open toward one side of the kit, and the magnetic capture head (72) is located beside the nucleic acid extraction and amplification kit (10).

12. The detection apparatus for nucleic acid extraction and amplification according to claim 1, wherein a reading module (100) is disposed beside the temperature-dependent amplification module (90), the reading module (100) comprises a reading support (101) disposed on the carrying platform (20), a reading electric cylinder (102) disposed on the reading support (101), a reading head fixing block (103) driven by the reading electric cylinder (102), and at least one reading head (104) disposed on the reading head fixing block (103), and the reading head (104) receives an optical signal of the amplification part.

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