CN112300927A - Portable full-automatic micro-fluidic nucleic acid detects all-in-one - Google Patents
Portable full-automatic micro-fluidic nucleic acid detects all-in-one Download PDFInfo
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
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Abstract
The invention relates to a portable full-automatic micro-fluidic nucleic acid detection all-in-one machine, which belongs to the technical field of nucleic acid detection and comprises a shell and a detection and analysis system arranged in the shell, wherein the detection and analysis system comprises a detection card loading module used for positioning a detection card, a nucleic acid extraction module used for pretreating reagents in the detection card, a nucleic acid amplification module used for amplifying the reagents in the detection card after pretreatment and a nucleic acid detection module used for detecting the reagents in the detection card after extraction and amplification are finished, a detection area of the detection card is positioned in a nucleic acid amplification cavity of the nucleic acid amplification module, the nucleic acid amplification module is connected with the nucleic acid detection module through an optical fiber line, the nucleic acid detection module comprises a power assembly used for pretreating the reagents in the detection card, and the power assembly is used for solving the problem that the detection environment requirement of the nucleic acid detection technology in the prior art is high, The full-closed detection can not be realized, and the detection efficiency and the detection precision need to be improved.
Description
Technical Field
The invention relates to a portable full-automatic micro-fluidic nucleic acid detection all-in-one machine, and belongs to the technical field of nucleic acid detection.
Background
Nucleic acid detection is a main technical means of molecular diagnosis, and has been widely applied to clinical detection of various respiratory pathogens. Mainstream nucleic acid detection generally requires a professional molecular biology laboratory, the pre-experimental treatment and detection steps for nucleic acid detection are separated and too complex and time-consuming, the requirement on the professional level of technicians is high, and the risk of infection (including operator infection, laboratory pollution and the like) is high. Meanwhile, the instruments are often complex in structure, large in occupied space, high in manufacturing cost, complex in operation and long in reporting period, so that the instruments are difficult to popularize in small medical units in common villages and towns. At present, some miniaturization detection is promoted in the market, but the miniaturization and integration are carried out on a large scale, the whole process is still consistent with that of the traditional method, and the popularization and the use on the basic level are difficult; secondly, some POCT (in-time testing) molecular diagnostic instruments are also introduced in the market, which are intended to realize an ideal mode of sample in and sample out, but in clinic, the full-closed detection in the true sense cannot be realized, and the instrument still has a high infection risk, so the instrument still needs to be carried out in a professional molecular biology laboratory, the cross risk and the use scene are limited, and the actual effect expectation of sample in and sample out is not achieved on the whole, and the main reason is that: the sealing of molecular diagnosis and detection is divided into 3 grades, and the difficulty is gradually increased. Firstly, amplification and detection product sealing are realized; secondly, the extraction, amplification and detection are closed; and thirdly, the whole process from sample to extraction, amplification and detection is closed. In conclusion, a novel molecular diagnosis technology is urgently needed in the market, which can realize the 3 rd level totally-enclosed nucleic acid detection from sample to extraction, amplification and detection, does not need a professional molecular biology laboratory, has extremely low technical requirements on operators, is suitable for various detection scenes (including basic layers), can realize the optimal solution mode of instant, efficient, high-precision, high-sensitivity, low-risk and low-cost nucleic acid detection, and achieves the purpose of sample input and result output in a real sense.
Disclosure of Invention
The invention provides a portable full-automatic micro-fluidic nucleic acid detection all-in-one machine aiming at the defects in the prior art, and aims to solve the technical problems that the detection environment requirement is high, the full-closed detection cannot be realized and the detection efficiency and the detection precision need to be improved in the nucleic acid detection technology in the prior art.
The technical scheme for solving the technical problems is as follows: the utility model provides a portable full-automatic micro-fluidic nucleic acid detects all-in-one, includes the casing and installs the detection and analysis system in the casing, detection and analysis system is including the detection card year module that is used for fixing a position the detection card, be used for carrying out the nucleic acid extraction module of preliminary treatment to the reagent in the detection card, be used for carrying out the nucleic acid amplification module that amplifies to the reagent in the detection card after the preliminary treatment is accomplished and be used for carrying out the nucleic acid detection module that detects to the reagent in the detection card after extracting and amplifying, the detection zone of detection card is located the nucleic acid amplification intracavity of nucleic acid amplification module, nucleic acid amplification module and nucleic acid detection module pass through the fiber optic line and are connected, the nucleic acid extraction module is including the power component that is used for carrying out the preliminary treatment to the reagent in the detection card.
On the basis of the technical scheme, the invention can be further improved as follows.
Further, power component includes that fixed mounting draws motor, magnet adsorption motor and micropump inside the casing, should draw the motor and detect the pneumatic valve and the liquid valve rotation on the card through the gear train drive, and this magnet adsorption motor passes through lead screw drive adsorption magnet and detects the adsorption magnet backup pad reciprocating motion of card along the perpendicular to, and this micropump passes through the air inlet intercommunication of gas circuit with the detection card.
Further, the gear train includes driving gear and two driven gears with drawing motor coaxial arrangement, and driving gear and two driven gears all rotate to be installed inside the casing, and two driven gears all mesh with the driving gear, and fixture block is all installed to two driven gear tip, and two fixture blocks insert the installation with the pneumatic valve and the liquid valve of detection card respectively.
Further, the nucleic acid extraction module still includes the centre gripping subassembly, and this centre gripping subassembly includes the centre gripping motor of fixed mounting on the casing, the guide rail reciprocating motion on the casing is followed to centre gripping motor through belt drive centre gripping moving platform, the last installation of centre gripping moving platform is used for holding spacing limiting plate to the detection card.
Further, the nucleic acid amplification module comprises an amplification support frame arranged on the shell, a nucleic acid amplification cavity for inserting the detection card and an amplification heating part for heating the nucleic acid amplification cavity are arranged on the amplification support frame, the amplification heating part comprises a temperature raising and lowering mechanism and a temperature control mechanism, a detection optical fiber group is further arranged on the amplification support frame, one end of the detection optical fiber group is communicated to the nucleic acid amplification cavity, and the other end of the detection optical fiber group is connected to the nucleic acid detection module through an optical fiber line.
Further, the nucleic acid amplification module further includes a heat radiating section for radiating heat to the amplification heating section, and the heat radiating section is attached to the amplification heating section side.
Further, the nucleic acid detection module comprises a motor support frame arranged on the shell, a rotating motor is arranged on the motor support frame, the rotating motor drives the rotating disk to rotate, a plurality of groups of light source emitting parts used for emitting light sources and sensor receiving parts used for receiving nucleic acid detection signals are arranged on the rotating disk, and the light source emitting parts and the sensor receiving parts are provided with interfaces in contact connection with optical fiber lines on the motor support frame.
Further, it includes that the card male loading support is detected to the detection card loading module, the fixture block passes the side of loading support, loading support installs on the casing bottom plate and is located between centre gripping moving platform and the driven gear, and it is outside that the door frame of the injection chamber that just loads support one end opening set up on through the casing communicates, and other end intercommunication nucleic acid amplification chamber, set up at least one boss on the limiting plate on the centre gripping moving platform, the one side that is close to the boss on the loading support sets up the through-hole that supplies the boss to pass.
Further, the detection card loading module further comprises a movable door used for shielding a door frame of the sample injection door, the movable door is driven by a motor to reciprocate along the inner side wall of the shell, the motor is installed on the movable door, the motor and a movable gear are coaxially installed, and the movable gear is meshed with a rack fixed on the inner wall of the shell.
Furthermore, the detection and analysis system also comprises a main control module used for data acquisition, signal processing and control of each module and a power supply module used for providing electric energy for each part.
Further, the casing outside still sets up touch display module, sweeps a yard module, printer module and external interface module, touch display module is used for showing the data information after the signal processing in real time, it is used for acquireing bar code, two-dimensional code, figure and colour information to sweep a yard module, and the production batch number, the detection type and the personal information of automatic identification detection card, printer module adopts miniature thermal printer for the output prints the report list, external interface module is used for connecting external equipment, supports wireless network and is connected with external equipment.
The invention has the beneficial effects that: the instrument has the advantages of light weight, small volume, easy portability, simple structure, extremely low cost, high reliability, simple and quick operation, high efficiency, high precision, high sensitivity, low risk and low cost, extremely low professional requirement on operation technicians, no need of professional doctors, realization of the totally-enclosed nucleic acid detection from sample to extraction, amplification and detection, realization without professional molecular biology laboratories, wide application, suitability for various scenes (basic level), emergency departments of up to three hospitals, community and town health hospitals, and particular suitability for the environment where the conventional instrument is not suitable for working at present, and realization of a real 'sample inlet and result outlet' molecular POCT product; and the consumable detection card is totally closed, is disposable, has no secondary pollution, is uniformly destroyed after being used, reduces the pollution to the environment to the maximum extent, does not generate radiation to a living body, and is green and environment-friendly.
Drawings
FIG. 1 is a front perspective view of the present invention;
FIG. 2 is a rear perspective view of the present invention;
FIG. 3 is a perspective view of the test card;
FIG. 4 is a schematic view of the loading of the detection card of the present invention;
FIG. 5 is a schematic diagram of a detection and analysis system;
FIG. 6 is a schematic diagram of the structure of a nucleic acid extraction module;
FIG. 7 is a schematic diagram of the structure of a nucleic acid amplification module;
FIG. 8 is a schematic diagram of the structure of a nucleic acid detecting module.
In the figure, 1, a touch display module, 2, a printer module, 3, a shell, 4, a code scanning module, 5, a sample injection cabin door, 6, a start button, 8, an external interface module, 10, a power supply interface, 11, a heat dissipation hole, 12, a sample tube, 13, a detection card, 14, a detection card loading module, 15, a nucleic acid extraction module, 16, a nucleic acid amplification module, 17, a power supply module, 18, a nucleic acid detection module, 19, a main control module, 1301, an air valve, 1302, a liquid valve, 1501, an extraction motor, 1502, a gear set, 1503, an adsorption magnet support plate, 1504, a limiting plate, 1505, an air circuit solenoid valve, 1506, a micro pump, 1507, a clamping limiting sensor, 1508, a clamping motor, 1509, a clamping moving platform, 1510, a guide rail, 1511, an adsorption magnet motor, 1512, a clamping block, 1601, an amplification support frame, 1602, a detection optical fiber set, 1604, a heat dissipation part, 1605, a, 1606. amplification heating part, 1801, rotating motor, 1804, light source emitting part, 1805, sensor receiving part, 1807, rotating disk, 1808, motor support frame.
Detailed Description
The principles and features of this invention are described below in conjunction with the following drawings, which are set forth by way of illustration only and are not intended to limit the scope of the invention.
A portable full-automatic micro-fluidic nucleic acid detection all-in-one machine comprises a shell 3 and a detection analysis system installed in the shell 3, wherein heat dissipation holes 11 are formed in the shell 3, a touch display module 1, a code scanning module 4, a printer module 2 and an external interface module 8 are further arranged outside the shell 3, the touch display module 1 is used for displaying data information after signal processing in real time, the code scanning module 4 is used for acquiring bar codes, two-dimensional codes, graphs and color information and automatically identifying production lot numbers, detection types and personal information of a detection card 13, the printer module 2 adopts a micro thermal printer and is used for outputting printing report sheets, the external interface module 8 is used for connecting external equipment and supporting a wireless network to be connected with the external equipment, the detection card 13 is used for processing, distributing, reacting and detecting samples to be detected and is a multipurpose multi-index micro-fluidic detection card 13, the sample to be tested includes, but is not limited to, various fluid samples, such as blood, plasma, serum, tissue fluid, lymph fluid, cerebrospinal fluid, semen, saliva, urine and other body fluid or environmental water samples or various liquid extracts.
The detection analysis system comprises a detection card loading module 14 for positioning the detection card 13, a nucleic acid extraction module 15 for pretreating reagents in the detection card 13, a nucleic acid amplification module 16 for amplifying the reagents in the detection card 13 after pretreatment, and a nucleic acid detection module 18 for detecting the reagents in the detection card 13 after extraction and amplification, wherein the detection area of the detection card 13 is positioned in a nucleic acid amplification cavity 1605 of the nucleic acid amplification module 16, the nucleic acid amplification module 16 is connected with the nucleic acid detection module 18 through an optical fiber line, and the detection analysis system has the advantages of light weight, small volume, easy portability, simple structure, extremely low cost, high reliability, simple and quick operation, high efficiency, high precision, high sensitivity, low risk and low cost, extremely low professional requirement on an operation technician, no need of a professional physician, and realization of extraction from a sample to a sample, The full-closed nucleic acid detection of amplification and detection can be realized without a professional molecular biology laboratory, has wide application, is suitable for various scenes (basic level), emergency departments of three hospitals, communities and villages and towns, is particularly suitable for the environment where the conventional instrument is not suitable for working at present, and realizes a real molecular POCT product with sample input and result output; the consumable detection card is totally closed, is disposable, has no secondary pollution, is uniformly destroyed after being used, reduces the pollution to the environment to the maximum extent, does not generate radiation to a living body, and is green and environment-friendly.
The nucleic acid extraction module 15 comprises a power assembly for pretreating reagents in the detection card 13, the power assembly comprises an extraction motor 1501, a magnet adsorption motor 1511 and a micro pump 1506, which are fixedly mounted inside the housing 3, the extraction motor 1501 drives an air valve 1301 and a liquid valve 1302 on the detection card 13 to rotate through a gear set 1502, the magnet adsorption motor 1511 drives an adsorption magnet to reciprocate along an adsorption magnet support plate 1503 perpendicular to the detection card 13 through a lead screw, the micro pump 1506 is communicated with an air inlet of the detection card 13 through an air passage provided with an air passage electromagnetic valve 1505, and is used for providing driving power for the detection card 13 through the micro pump 1506 and realizing the actions of flowing, mixing, quantifying, distributing and the like of a sample to be detected in a flow passage and a cavity of the detection card 13. The gear set 1502 comprises a driving gear and two driven gears which are coaxially mounted with the extraction motor 1501, the driving gear and the two driven gears are rotatably mounted inside the shell 3, the two driven gears are meshed with the driving gear, fixture blocks 1512 are mounted at the end portions of the two driven gears, the two fixture blocks 1512 are respectively inserted into and mounted on the air valve 1301 and the liquid valve 1302 of the detection card 13, and the fixture blocks 1512 can synchronously rotate with the air valve 1301 or the liquid valve 1302 after being inserted into the air valve 1301 or the liquid valve 1302. Nucleic acid extraction module 15 still includes the centre gripping subassembly, and this centre gripping subassembly includes clamping motor 1508 of fixed mounting on casing 3, clamping motor 1508 is through belt drive centre gripping moving platform 1509 along guide rail 1510 reciprocating motion on the casing 3, still install on the casing 3 and be used for carrying out spacing centre gripping spacing sensor 1507 to the stroke of centre gripping moving platform 1509, the installation is used for carrying out spacing limiting plate 1504 to detection card 13 centre gripping on the centre gripping moving platform 1509 for carry out spacing location to detection card 13, be convenient for subsequent extraction operation.
The nucleic acid amplification module 16 comprises an amplification support 1601 which is installed on the housing 3, a nucleic acid amplification chamber 1605 for inserting the detection card 13 and an amplification heating part 1606 for heating the nucleic acid amplification chamber 1605 are arranged on the amplification support 1601, the amplification heating part 1606 comprises a temperature raising and lowering mechanism and a temperature control mechanism, a detection optical fiber set 1602 is further installed on the amplification support 1601, one end of the detection optical fiber set 1602 is connected to the nucleic acid amplification chamber 1605, and the other end is connected to the nucleic acid detection module 18 through an optical fiber line. The nucleic acid amplification module 16 further includes a heat dissipation part 1604 for dissipating heat from the amplification heating part 1606, wherein the heat dissipation part 1604 is installed on one side of the amplification heating part 1606 and is used for realizing various preset temperatures required by nucleic acid expansion, and adjusting heating parameters in real time according to different heating states to realize optimal control.
The nucleic acid detection module 18 comprises a motor support frame 1808 installed on the housing 3, a rotating motor 1801 is installed on the motor support frame 1808, the rotating motor 1801 drives a rotating disk 1807 to rotate, a plurality of groups of light source emitting parts 1804 used for emitting light sources and sensor receiving parts 1805 used for receiving nucleic acid detection signals are installed on the rotating disk 1807, the light source emitting parts 1804 and the sensor receiving parts 1805 are both provided with interfaces in contact connection with optical fiber lines on the motor support frame 1808, the nucleic acid detection module 18 is an optical detection system, multi-wavelength detection is achieved, and the nucleic acid detection module is used for measuring and acquiring micro changes of multiple paths of samples to be detected in the whole reaction process in real time and converting the micro changes into digital signals convenient to process.
Detect and clamp loading module 14 including supplying to detect card 13 male loading support, fixture block 1512 passes the side of loading support, loading support installs on the casing 3 bottom plate and is located between centre gripping moving platform 1509 and the driven gear, and loads the outside 5 door frames intercommunication of the injection chamber door that support one end opening set up on through casing 3, other end intercommunication nucleic acid amplification chamber 1605, set up at least one boss on the limiting plate 1504 on the centre gripping moving platform 1509, the last one side that is close to the boss of loading support sets up the through-hole that supplies the boss to pass. The detection card loading module 14 further comprises a movable door used for shielding a door frame of the sample injection cabin door 5, the movable door is driven by a motor to reciprocate along the inner side wall of the shell 3, the motor is installed on the movable door, the motor and a movable gear are coaxially installed, the movable gear is meshed with a rack fixed on the inner wall of the shell, and the detection card loading module is used for bearing and accurately placing a detection card 13 of a sample to be detected and is prepared for testing.
The detection and analysis system also comprises a main control module 19 for data acquisition, signal processing and control of each module and a power module 17 for providing power for each part, wherein the power module 17 is provided with a power interface 10.
The use method of the portable full-automatic micro-fluidic nucleic acid detection all-in-one machine comprises the following steps: the method comprises the following steps: s1, firstly, the power of the equipment is turned on, the start button 6 is turned on, and the equipment waits for operation after power-on self-test; s2, inputting information of a patient to be detected, starting a test, automatically opening the sampling cabin door 5, inserting the sample tube 12 containing a sample to be detected into the detection card 13, then loading the detection card 13 into the instrument, and prompting a screen to click to start the detection; s3: the sample introduction cabin door 5 is automatically closed, the instrument enters into the test operation, and the processes of the sample to be tested flowing in the flow channel of the detection card 13, sample processing, quantitative distribution to the designated area, reaction, detection and the like are completed; s3, automatically opening the sample chamber door 5 after the detection is finished, displaying the detection result and printing a report sheet; and S4, completing the test task, and automatically resetting the instrument and finally electrically starting the instrument.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (10)
1. The utility model provides a portable full-automatic micro-fluidic nucleic acid detects all-in-one which characterized in that: the detection and analysis system comprises a shell and a detection and analysis system arranged in the shell, wherein the detection and analysis system comprises a detection card loading module, a nucleic acid extraction module, a nucleic acid amplification module for amplifying a reagent and a nucleic acid detection module, a detection area of the detection card is positioned in a nucleic acid amplification cavity of the nucleic acid amplification module, the nucleic acid amplification module is connected with the nucleic acid detection module through an optical fiber line, and the nucleic acid extraction module comprises a power assembly for pretreating the reagent in the detection card.
2. The portable full-automatic micro-fluidic nucleic acid detection all-in-one machine of claim 1, characterized in that: the power component comprises an extraction motor, a magnet adsorption motor and a micropump which are fixedly installed inside the shell, the extraction motor drives an air valve and a liquid valve on the detection card to rotate through a gear set, the magnet adsorption motor drives an adsorption magnet to reciprocate along an adsorption magnet supporting plate perpendicular to the detection card through a screw rod, and the micropump is communicated with an air inlet of the detection card through an air path.
3. The portable full-automatic micro-fluidic nucleic acid detection all-in-one machine of claim 2, characterized in that: the gear train includes driving gear and two driven gears with drawing motor coaxial arrangement, and driving gear and two driven gears all rotate to be installed inside the casing, and two driven gears all mesh with the driving gear, and fixture block is all installed to two driven gear tip, and two fixture blocks insert the installation with the pneumatic valve and the liquid valve of detection card respectively.
4. The portable full-automatic micro-fluidic nucleic acid detection all-in-one machine of claim 3, characterized in that: the nucleic acid extraction module further comprises a clamping assembly, the clamping assembly comprises a clamping motor fixedly mounted on the shell, the clamping motor drives the clamping moving platform to reciprocate along a guide rail on the shell through belt transmission, and a limiting plate used for limiting the detection card is mounted on the clamping moving platform.
5. The portable full-automatic microfluidic nucleic acid detection all-in-one machine according to any one of claims 1 to 3, wherein: the nucleic acid amplification module comprises an amplification support frame arranged on the shell, a nucleic acid amplification cavity used for inserting the detection card and an amplification heating part used for heating the nucleic acid amplification cavity are arranged on the amplification support frame, the amplification heating part comprises a temperature raising and lowering mechanism and a temperature control mechanism, a detection optical fiber group is further arranged on the amplification support frame, one end of the detection optical fiber group is communicated to the nucleic acid amplification cavity, and the other end of the detection optical fiber group is connected to the nucleic acid detection module through an optical fiber line.
6. The portable full-automatic micro-fluidic nucleic acid detection all-in-one machine of claim 5, characterized in that: the nucleic acid amplification module further includes a heat radiating portion for radiating heat from the amplification heating portion, and the heat radiating portion is attached to the amplification heating portion side.
7. The portable full-automatic micro-fluidic nucleic acid detection all-in-one machine of claim 6, characterized in that: the nucleic acid detection module comprises a motor support frame arranged on the shell, a rotating motor is arranged on the motor support frame, the rotating motor drives the rotating disk to rotate, a plurality of groups of light source emitting parts used for emitting light sources and sensor receiving parts used for receiving nucleic acid detection signals are arranged on the rotating disk, and the light source emitting parts and the sensor receiving parts are provided with interfaces in contact connection with optical fiber lines on the motor support frame.
8. The portable full-automatic micro-fluidic nucleic acid detection all-in-one machine of claim 7, characterized in that: the detection card loading module comprises a loading support for inserting a detection card, the clamping block penetrates through the side face of the loading support, the loading support is installed on a bottom plate of the shell and is located between the clamping moving platform and the driven gear, an opening at one end of the loading support is communicated with the outside through a door frame of a sample injection cabin door arranged on the shell, the other end of the loading support is communicated with the nucleic acid amplification cavity, at least one boss is arranged on a limiting plate on the clamping moving platform, and a through hole for the boss to pass through is formed in one side, close to the boss, of the loading support.
9. The portable full-automatic micro-fluidic nucleic acid detection all-in-one machine of claim 8, characterized in that: the detection card loading module further comprises a movable door used for shielding a door frame of the sample injection door, the movable door is driven by a motor to reciprocate along the inner side wall of the shell, the motor is installed on the movable door, the motor is coaxially installed with a movable gear, and the movable gear is meshed with a rack fixed on the inner wall of the shell.
10. The portable full-automatic micro-fluidic nucleic acid detection all-in-one machine of claim 9, characterized in that: the casing outside still sets up touch display module, sweeps a yard module, printer module and external interface module, touch display module is used for showing the data information after the signal processing in real time, sweep yard module and be used for acquireing bar code, two-dimensional code, figure and colour information, the production batch number, the detection type and the personal information of automatic identification detection card, printer module adopts miniature thermal printer for the output prints the report list, external interface module is used for connecting external equipment, supports wireless network and external equipment and is connected.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011374548.4A CN112300927A (en) | 2020-11-30 | 2020-11-30 | Portable full-automatic micro-fluidic nucleic acid detects all-in-one |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN202011374548.4A CN112300927A (en) | 2020-11-30 | 2020-11-30 | Portable full-automatic micro-fluidic nucleic acid detects all-in-one |
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