CN209024540U - A kind of digital pcr system - Google Patents
A kind of digital pcr system Download PDFInfo
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- CN209024540U CN209024540U CN201821678881.2U CN201821678881U CN209024540U CN 209024540 U CN209024540 U CN 209024540U CN 201821678881 U CN201821678881 U CN 201821678881U CN 209024540 U CN209024540 U CN 209024540U
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Abstract
The utility model relates to a kind of digital pcr systems, including pedestal, mobile mechanism on the base is set, operating platform on the base is set, drop formation mechanism with sampling probe, temperature control mechanism, product signal collecting mechanism, sample process mechanism and control mechanism, sample process mechanism includes the sample process consumptive material and sample process actuating mechanism with multiple independent cavitys, sample process actuating mechanism, drop formation mechanism, product signal acquisition module is respectively arranged in mobile mechanism and can be along the left and right directions of droplet type digital pcr system under the driving of mobile mechanism, front-rear direction movement, sample process mechanism, mobile mechanism, drop formation mechanism, nucleic acid amplification temperature control module, product signal acquisition module connect with control module respectively and by control mechanism controls.The structure design of the utility model is reasonable, and system bulk reduces, and user's operation is simple, and work efficiency is high, and detection cycle is shorter, and operating error is small.
Description
Technical field
The utility model relates to field of molecular detection, and in particular to a kind of digital pcr system.
Background technique
With the transformation of medical model and the continuous development of personalized medicine, there is an urgent need to quick, accurate for medical test circle
Detection means, wherein Molecular Detection have unique advantage.
Currently, molecular detection technology mainly has making nucleic acid molecular hybridization, polymerase chain reaction (PCR) and biochip technology
Deng.Molecular Detection product is mainly used in the detection and physical examination of the clinical departments such as tumour, infection, heredity, Prenatal Screening
The heart, technical service center, third party testing agency and microbial rapid detection market etc..
As the important technical of Molecular Detection, round pcr can qualitatively and quantitatively detect target nucleic acid molecules,
Under the application demand background that low abundance detection, rare mutation detection etc. increasingly increase, digital pcr is absolute as a kind of nucleic acid molecules
Quantitative technique, a quantitative fluorescent PCR reaction system is assigned in a large amount of small reactors by it, in each microreactor
Target nucleic acid molecules comprising one or more copies carry out " unimolecule template PCR amplifications ", after amplification, by the positive
The number and statistical method of reaction member calculate the copy number of target gene in original sample, and digital pcr need not rely on reference substance
Accurately absolute quantitation detection can be carried out with standard curve.
Currently, the measuring means such as blood routine, cytology, pathology and immunology are towards automation, integration, standardization
Direction develop, but due to Molecular Detection self-technique complexity, the automation from sample to result realize during there is
Many insoluble technical problems.It is single that the preceding acquisition of sample of nucleic acid and the pre-treatment step of sample of nucleic acid are reacted with regard to digital pcr
For, traditional approach requires more manual operations to participate in, and the degree of automation is low, and to the more demanding of use condition,
The operation equipment for having profession is asked just to can be carried out.In order to solve these problems, the prior art is it has been suggested that automatic nucleic acid extraction
Device, the device etc. that automatic nucleic acid extraction, amplification, detection are integrated.However, these apparatus structures are typically more complicated, and not
Enough compact, volume is big, user's operation is complicated, is unsuitable for constructing unitary drop formula digital pcr system.
Summary of the invention
Integrate sample process, drop formation, amplification and the number detected the purpose of the utility model is to provide a kind of
PCR system, the system structure is compact, equipment volume is small, is arranged simple, easy to use.
In order to achieve the above objectives, a kind of technical solution that the utility model uses is:
A kind of digital pcr system comprising mobile mechanism on the base is arranged in pedestal, and operation on the base is arranged
Platform, the drop formation mechanism with sampling probe, temperature control mechanism, product signal collecting mechanism and control mechanism, drop formation
Mechanism, product signal collecting mechanism are respectively arranged in mobile mechanism and can be along digital pcr systems under the driving of mobile mechanism
Left and right directions, front-rear direction movement, temperature control mechanism include for nucleic acid amplification heating the first heating component；
Digital pcr system further includes sample process mechanism, and the sample process mechanism includes one or more groups of pipettes, and one
Group or multiple groups sample process consumptive material and sample process actuating mechanism, in which: sample process consumptive material includes being arranged in operating platform
On substrate and one or more processing units on base material are set, each processing unit includes multiple cavitys being independently arranged；
Sample process actuating mechanism includes the first attachment base that can slide up and down to setting, for driving the first attachment base to slide up and down
First longitudinal direction mobile device, connect for being removably installed pipette with the first attachment base or first longitudinal direction mobile device
Liquid relief pipe connector, the first liquid relief driving device for driving pipette progress imbibition and drain movement, sample process movement
Mechanism is set in the mobile mechanism and can be along the right and left of the digital pcr system under the driving of the mobile mechanism
It is moved to, front-rear direction；
The mobile mechanism, sample process actuating mechanism, nucleic acid amplification temperature control mechanism, product signal collecting mechanism respectively with
Control mechanism connects and by control mechanism controls.
Preferably, the opening up setting of multiple cavities.
Preferably, multiple cavities are spaced apart and are arranged side by side, the height side of the axial line of each cavity respectively along substrate
To extension.
According to the utility model, the cavity number of each processing unit is not particularly limited, and specifically, the number of cavity can be comprehensive
It closes and considers that the factors such as nucleic acid extracting reagent type determine.In a preferred aspect according to the present utility model, each processing unit point
It Bao Kuo not 6 ~ 20 cavitys, particularly preferably 8 ~ 15 cavitys.
A preferred aspect according to the present utility model is also formed with the support portion for placing pipette on substrate.
Another preferred aspect according to the present utility model is also formed with the support portion for placing sampling probe on substrate.
Preferably, the temperature control mechanism further includes the second heating component heated for liquid in cavity, the second heating group
Part includes heating coil.
Preferably, the sample process mechanism further includes magnet.Using magnet and magnetic bead, it can be achieved that nucleic acid separates.
Preferably, heating coil, magnet are able to movably be arranged.
Preferably, the first heating component, the second heating component are respectively positioned below operating platform, heating coil, magnet difference
It can slide up and down to across operating platform.
Preferably, in each processing unit, have and be provided with heat conducting element on the corresponding substrate of one or more cavitys.It can move
Dynamic heating coil, contacts it with heat conducting element and conducts heat, to accelerate to crack.One according to the present utility model specific side
Face, heat conducting element are preferably elongated shape, are at least partially situated at the axis in cavity and transverse to cavity.The setting is led
Thermal element can also play the effect of flow-disturbing, be conducive to the mixing of liquid in the cavity.
Preferably, in each processing unit, have and be provided with magnetic conductive component on the corresponding substrate of one or more cavitys.It can move
Dynamic magnet, contacts it with magnetic conductive component and is conducted, to preferably carry out magnetic bead absorption.One according to the present utility model
Specific aspect, magnetic conductive component are elongated shape, are at least partially situated at the axis in cavity and transverse to cavity.The setting
Magnetic conductive component can also play the effect of flow-disturbing, be conducive to the mixing of liquid in the cavity.
A specific aspect according to the present utility model is provided with the heat conducting element at one cavity, another cavity
Place's setting magnetic conductive component.Preferably, setting heat conducting element is adjacent with the cavity of magnetic conductive component.
Preferably, the sample process consumptive material further includes the reagent consumptive material with reagent storage chamber, the reagent storage chamber
It is connected to a cavity in the multiple cavity by microchannel.
It is further preferred that the reagent consumptive material and the substrate are detachably connected.
In some specific embodiments according to the present utility model, interface, the reagent are provided on the substrate
Consumptive material include shell, be arranged in the intracorporal U-shaped Reagent Tube of the shell, setting on the housing with the interface of the substrate match
A pair of of plug of grafting is closed, the inner cavity of the Reagent Tube constitutes the reagent storage chamber, the both ends difference of the Reagent Tube
It is connected with micro-pipe, two micro-pipes are each passed through the pair of plug setting, when the reagent consumptive material is connect with the substrate
When, one inner cavity in two micro-pipes, which is constituted, connects the inner cavity of the Reagent Tube and the bottom of a cavity
Microchannel, for connecting drive module, the drive module be the device that can drive liquid flowing for another.
According to the utility model, the microchannel is preferably disposed to when without outer power drive or driving force is lower than setting value
When liquid the channel of another chamber will not be moved to from a chamber.The aperture of microchannel is, for example, 60 microns ~ 100 microns,
Preferably 70 ~ 100 microns.
A specific aspect according to the present utility model, the multiple cavities of each processing unit along digital pcr system length
It spends direction to be arranged side by side, drop formation mechanism is set with sample process actuating mechanism along the width direction of digital pcr system side by side
It sets.
According to the utility model, the drop formation mechanism is not particularly limited, can using it is those of known or according to
Known drop formation mechanism accordingly designs.The utility model is preferably raw using the drop for generating drop using microchannel vibration
At mechanism.
A specific aspect according to the present utility model, drop formation mechanism include can slide up and down to setting second
Attachment base, the oscillation drive being arranged on the second attachment base, the second longitudinal direction for driving the second attachment base to slide up and down
Mobile device, be arranged on oscillation drive for being removably installed the sampling probe connector of sampling probe, for driving
Sampling probe carries out the second liquid relief driving device of imbibition and drain movement, is additionally provided with drop receptacle mounting portion on operating platform, leads to
It crosses drop receptacle mounting portion and is removably installed drop receptacle.When needing to generate drop, oily phase is first placed in drop receptacle, it will
The sampling probe for having drawn sample solution is inserted under oil phase liquid face, by the second liquid relief driving device driving by sample solution from
The end of sampling probe is released, at the same oscillation drive driving sampling probe do it is of reciprocating vibration.This kind of drop formation method can produce
Raw uniform drop and flux height.
One according to the present utility model specific and preferred aspect, mobile mechanism include the length along micro- digital pcr system
The X direction guiding rail that direction extends, the mobile pedestal being slidably connected with X direction guiding rail, setting is on mobile pedestal and along digital pcr system
The Y-direction guide rail that extends of width direction, the sliding seat being slidably connected with Y-direction guide rail is respectively used to drive mobile pedestal, sliding seat
The first motor driving device and the second motor driver of sliding, drop formation mechanism, product signal acquisition module are set respectively
It sets on sliding seat.
It is further preferred that the sliding seat includes the fixed block being uprightly arranged, it is respectively arranged on fixed block along upper
The first sliding rail, the second sliding rail of lower direction extension, the first attachment base of above-mentioned sample process actuating mechanism, above-mentioned drop are raw
It is arranged side by side at the second attachment base of mechanism, and the two is slidably connected with the first sliding rail, the second sliding rail respectively.
According to the utility model, the first liquid relief driving device, the second liquid relief driving device are not particularly limited, can
Using known fluid drive apparatus.
Due to the application of the above technical scheme, the utility model has the advantage that compared with prior art
The digital pcr system collection sample process of the utility model droplet formation, expands, is detected on one, realizes from sample
Handle the automation control that drop formation arrives result detection to PCR reaction again.The structure design of the utility model is reasonable, and system is whole
Body volume reduces, meanwhile, reduce user's operation complexity, improve work efficiency, shorten detection cycle, reduces behaviour
Make error.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the digital pcr system of embodiment；
Fig. 2 is the sample process actuating mechanism of embodiment and the structural schematic diagram of drop formation mechanism；
Fig. 3 is the partial structural diagram of digital pcr system；
Fig. 4 is the schematic front view of the sample process consumptive material of embodiment；
Fig. 5 is the schematic top plan view of the sample process consumptive material of embodiment；
Fig. 6 is the schematic cross-sectional view at the A-A in Fig. 5；
Wherein: 1, pedestal；2, operating platform；21, drop receptacle mounting portion；3, drop formation mechanism；300, sampling probe；
310, drop receptacle；320, the second attachment base；330, oscillation drive；340, second longitudinal direction mobile device；350, sampling probe
Connector；360, the second liquid relief driving device；42, the second heating component；5, product signal collecting mechanism；700, pipette；
710, sample process actuating mechanism；711, the first attachment base；712, first longitudinal direction mobile device；713, liquid relief pipe connector；
714, the first liquid relief driving device；720, sample process consumptive material；721, substrate；722, cavity；723, heat conducting element；724, magnetic conduction
Element；725, reagent consumptive material；7251, shell；7252, Reagent Tube；7253, plug；7254, micro-pipe；726, drive module；91,X
Direction guiding rail；92, mobile pedestal；93, Y-direction guide rail；94, sliding seat；95, fixed block；951, the first sliding rail；952, the second sliding rail.
Specific embodiment
The utility model provides a kind of digital pcr system, the droplet type digital pcr system collection nucleic acid extraction and processing,
Droplet formation expands, is detected on one.The digital pcr system of the utility model is realized to expand from sample process, drop formation, PCR
The automation control of detection after increasing reaction and reaction not only avoids manual operation, reduces work time-consuming, also improves result
Accuracy.
The technical solution of the utility model is further elaborated with specific embodiment with reference to the accompanying drawing.It should
Understand, specific embodiment described herein only to explain the utility model, is not used to limit the utility model.
As shown in Figure 1 and Figure 2, a specific embodiment according to the present utility model, digital pcr system include pedestal 1, if
The mobile mechanism on pedestal 1 is set, the operating platform 2 on pedestal 1 is set, the drop formation mechanism 3 with sampling probe 300,
Temperature control mechanism, product signal collecting mechanism 5, sample process mechanism and control mechanism (not shown).Mobile mechanism, liquid
Drop generating mechanism 3, temperature control mechanism, product signal collecting mechanism 5 etc. connect with control mechanism respectively and by control mechanism controls.
Sample process mechanism includes multiple pipettes 700, sample process actuating mechanism 710 and multiple sample process consumption
Material 720, in which: each sample process consumptive material 720 includes the substrate 721 being arranged on operating platform 2, is arranged on substrate 721
Processing unit and reagent consumptive material 725.The processing unit includes multiple cavitys 722 being independently arranged, these cavitys 722 are for putting
Set various substances, such as cleaning solution, cell pyrolysis liquid, enzyme, eluent, magnetic bead needed for extracting nucleic acid etc..The opening of cavity 722 court
On setting, multiple cavities 722 are intervally arranged along the length direction of substrate 721.The axial line of each cavity 722 is respectively along base
The short transverse of material 721 extends, and the length direction of substrate 721 is consistent with the length direction of digital pcr system (as shown in Figure 1).
Reagent consumptive material 725 and substrate 721 are mutually detachably connected, referring specifically to fig. 6: interface, reagent consumptive material are provided on substrate 721
725 include shell 7251, the U-shaped Reagent Tube 7252 being arranged in shell 7251, be arranged on shell 7251 and connect with substrate 721
Mouth matches a pair of of plug 7253 of grafting, and the inner cavity of U-shaped Reagent Tube 7252 constitutes reagent storage chamber, U-shaped Reagent Tube 7252
Both ends are connected separately with micro-pipe 7254, and two micro-pipes 7254 are each passed through a pair of of plug 7253 and are arranged, when reagent consumptive material 725 and base
When material 721 connects, one inner cavity in two micro-pipes 7254 is constituted the cavity on the inner cavity of Reagent Tube 7252 and substrate 721
The microchannel (aperture is about 100 microns) that 722 bottom is connected, another is for connecting drive module 726 or atmosphere.Drive mould
Block 726 is that can drive the device that liquid flows in reagent storage chamber, can be driving device commonly used in the art, such as uses gas
The driving of body transfer tube completion liquid.Drive module 726 connect and is controlled with control mechanism.
Referring to Fig. 1, Fig. 2, sample process actuating mechanism 710 include can slide up and down to setting the first attachment base 711,
First longitudinal direction mobile device 712 and the first attachment base 711 or first longitudinal direction for driving the first attachment base 711 to slide up and down
Mobile device 712 connection for be removably installed pipette 700 liquid relief pipe connector 713, for drive pipette 700 into
First liquid relief driving device 714 of row imbibition and drain movement.
The first liquid relief driving device 714 for driving pipette 700 to carry out imbibition and drain movement is not particularly limited,
It can be driving device commonly used in the art, such as complete to draw and release liquid using syringe pump.For driving the first attachment base
The 711 first longitudinal direction mobile devices 712 slided up and down it is not also specifically limited, can using motor driven leadscrew-nut mechanism or
Motor driven rack pinion structure realizes, preferred motor driven leadscrew-nut mechanism, these mechanisms it is specific be arranged be it is conventional,
Herein without repeating.
722 numbers of cavity of each processing unit are not particularly limited, and specifically, the number of cavity 722 can comprehensively consider core
Acid extracts the factors such as reagent type and determines；In a preferred aspect according to the present utility model, each processing unit respectively includes 6 ~
20 cavitys 722, particularly preferably 8 ~ 15 cavitys 722.In the present embodiment, 722 total number of cavity of each processing unit is 8
It is a.
In the present embodiment, the support portion and sampling probe 300 for placing pipette 700 are also provided on substrate 721
Support portion.As shown in fig. 6, support portion is the supported hole extended along the short transverse of substrate 721, or may be set to be
Cavity form, pipette 700 and sampling probe 300 are respectively inserted in corresponding supported hole.
In the present embodiment, the sample process mechanism further includes that magnet (not shown) can using magnet and magnetic bead
Realize nucleic acid separation.
In the present embodiment, temperature control mechanism includes the first heating component (not shown), the use for nucleic acid amplification heating
In the second heating component 42 that liquid in cavity 722 heats, the second heating component 42 includes heating coil (not shown).Add
Heat coil, magnet are able to movably be arranged, and the first heating component, the second heating component 42 are respectively positioned on 2 lower section of operating platform,
Heating coil, magnet are fixed on a sliding shoe, which can slide up and down to across operating platform 2.
As shown in figs. 4 and 6, in each processing unit, have and be provided with heat conducting element on the corresponding substrate 721 of cavity 722
723, magnetic conductive component 724 is provided on the corresponding substrate 721 of a cavity 722 adjacent thereto.Made by mobile heating coil
It is contacted with heat conducting element 723 conducts heat, to accelerate the cracking of nucleic acid, moving magnet contacts it with magnetic conductive component 724
It is conducted, to preferably carry out magnetic bead absorption.In the present embodiment, heat conducting element 723, magnetic conductive component 724 are respectively thin
Long shape, wherein heat conducting element 723, magnetic conductive component 724 are respectively at least partially located in cavity 722 and transverse to cavity 722
Axis, heat conducting element 723, the magnetic conductive component 724 of the setting can play the effect of flow-disturbing, be conducive to liquid in the cavity 722
The mixing of body.Heat conducting element 723 and magnetic conductive component 724, more convenient operation, structure design is respectively set in adjacent two cavitys 722
It is simpler.
In this example, drop formation mechanism generates the generating mode of drop using microchannel vibration.As shown in Fig. 2, drop is raw
It include that can slide up and down to the second attachment base 320 of setting, the vibratory drive being arranged on the second attachment base 320 at mechanism 3
Device 330, is arranged in oscillation drive the second longitudinal direction mobile device 340 for driving the second attachment base 320 to slide up and down
On 330 for be removably installed sampling probe 300 sampling probe connector 350, for drive sampling probe 300 carry out imbibition and
Second liquid relief driving device 360 of drain movement is led to as shown in figure 3, being additionally provided with drop receptacle mounting portion 21 on operating platform 2
It crosses drop receptacle mounting portion 21 and is removably installed drop receptacle 310.When needing to generate drop, first put in drop receptacle 310
Oily phase is set, the sampling probe 300 for having drawn sample solution is inserted under oil phase liquid face, passes through the second liquid relief driving device 360
Driving releases sample solution from the end of sampling probe 300, while oscillation drive 330 drives sampling probe 300 to do reciprocal vibration
Dynamic, this kind of drop formation method can produce uniform drop and flux is high.There is no limit preferably for drop receptacle 310
The container of seal form.
According to the utility model, for driving sampling probe 300 to vibrate oscillation drive can there are many forms, preferably
Including can be realized 300 short stroke high speed of microchannel vibratory equipment of reciprocating vibration, moving back and forth, which can be, up and down reciprocatingly vibrates,
Reciprocating linear vibration in left and right rotates left and right reciprocating motion, and vibratory equipment can use electromagnet type, piezoelectric ceramic type or machinery
The equipment such as eccentric wheel type, oscillating cylinder, rotating electromagnet；For driving sampling probe 300 to carry out the second of imbibition and drain movement
Liquid relief driving device 360 is not particularly limited, and can be driving device commonly used in the art, such as using syringe pump complete draw and
Release liquid；Second longitudinal direction mobile device 340 for driving the second attachment base 320 to slide up and down is not particularly limited, can be with
It is realized using motor driven leadscrew-nut mechanism or motor driven rack pinion structure, preferably motor driven leadscrew-nut mechanism,
These mechanisms it is specific setting be it is conventional, herein without repeating.
As shown in Figure 1, mobile mechanism include along micro- digital pcr system length direction extend X direction guiding rail 91, with X to
The Y-direction extended on mobile pedestal 92 and along the width direction of digital pcr system is arranged in the mobile pedestal 92 that guide rail is slidably connected
Guide rail 93, the sliding seat 94 being slidably connected with Y-direction guide rail 93 are respectively used to drive that mobile pedestal 92, sliding seat 94 slide the
One motor driver (not shown) and the second motor driver (not shown).Further, sliding seat 94 includes uprightly setting
The fixed block 95 set is respectively arranged with the first sliding rail 951, the second sliding rail 952 extended along up and down direction, sample on fixed block 95
First attachment base 711 of present treatment actuating mechanism 710, drop formation mechanism 30 the second attachment base 320 along the digital pcr
The width direction of system is arranged side by side, and the two is slidably connected with the first sliding rail 951, the second sliding rail 952 respectively.So set,
Drop formation mechanism 3 and sample process actuating mechanism 710 can carry out the movement of front and rear, left and right and up and down direction.
Product signal acquisition module 5, including camera, optical fiber, exciting light turbin generator etc. specifically can be used this field and routinely set
Set mode.Product signal acquisition module 5 is arranged on sliding seat 94, and front and rear, left and right direction can be carried out by being driven by mobile mechanism
It is mobile.
Include: using the operating procedure that the digital pcr system of the present embodiment is detected
Substance needed for being packed into extraction nucleic acid in different cavitys 722 on sample process consumptive material 720 (including cleaning solution,
Lysate, enzyme such as Proteinase K, eluent, magnetic bead etc.) and measuring samples, and corresponding dosing pipette 700 and sampling probe 300,
Wherein, measuring samples are fitted into the cavity for being provided with heat conducting element 723.The operation of digital pcr system is set in operation sequence
Relevant parameter；Control mechanism controls mobile mechanism by relevant instruction information and sample process actuating mechanism 710 is driven to be moved to
Above pipette on substrate 721, make the liquid relief pipe connector 713 and 700 knot of pipette in sample process actuating mechanism 710
It closes, liquid relief pipe connector 713 and pipette 700 is driven to move up reset again later, complete the installation of pipette 700.
Nucleic acid extraction process: control mechanism drives sample process actuating mechanism 710 from the chamber for filling lysate and Proteinase K
Lysate and Proteinase K are drawn in body and is transferred to them in the cavity for being provided with heat conducting element 723 mixes with sample therein
It closes, heating is cracked；After cracking, control driving sample process actuating mechanism 710, which moves to the liquid after cracking, to be equipped with
It carries out mixing and combining with magnetic bead in the cavity of magnetic bead, after the completion of magnetic bead absorption, control driving sample process actuating mechanism 710 will
Waste liquid in this cavity removes, and cleaning solution is moved to the cavity equipped with magnetic bead from the cavity equipped with cleaning solution and is carried out to magnetic bead
Cleaning after cleaning, then controls driving sample process actuating mechanism 710 and removes waste liquid from the cavity, then wash from being equipped with
Eluent the cavity equipped with magnetic bead is moved in the cavity of de- liquid to elute nucleic acid, after elution still through magnetic bead into
Row absorption is moved to and reagent consumptive material 725 after the completion of absorption by driving sample process actuating mechanism 710 to draw quantitative nucleic acid
In the cavity of connection.
Prepare the sample (or nucleic acid amplification reaction liquid) of digital pcr detection: by drive module 726 by reagent consumptive material
Reagent in 725 is pushed into the cavity equipped with nucleic acid solution, and is mixed with nucleic acid solution, and digital pcr detection is obtained
Sample.
It is driven on drop formation mechanism 3 to substrate where sampling probe 300 after completing sample process by control mobile mechanism
Position after sampling probe 300, then is moved to the position of drop receptacle, and sampling probe 300 is inserted into oil phase liquid face in drop receptacle
Lower section starts to be vibrated and pushed away sample, makes the drop that uniform size is generated in drop receptacle.After drop formation, the first heating
Component is begun to warm up, progress nucleic acid amplification, and after the completion of heat cycles, product signal acquisition module 5 is moved to where drop receptacle
Position is observed, shoots photo, is sent to control module and carries out data processing and analysis.
The above embodiments are only for explaining the technical ideas and features of the present invention, and its object is to allow be familiar with technique
Personage can understand the content of the utility model and be implemented, do not limit the protection scope of the present invention,
All equivalent change or modifications according to made by the spirit of the present invention essence, should all cover in the protection scope of the utility model
It is interior.
Claims (19)
1. a kind of digital pcr system, it is characterised in that: including pedestal, the mobile mechanism on the pedestal is arranged in, is arranged in institute
The operating platform on pedestal is stated, the drop formation mechanism with sampling probe, temperature control mechanism, product signal collecting mechanism, and control
Mechanism processed, the drop formation mechanism, the product signal collecting mechanism are respectively arranged in the mobile mechanism and in the shifting
It can be moved along left and right directions, the front-rear direction of the digital pcr system under the driving of motivation structure, the temperature control mechanism includes using
In the first heating component of nucleic acid amplification heating；
The digital pcr system further includes sample process mechanism, and the sample process mechanism includes one or more groups of pipettes, and one
Group or multiple groups sample process consumptive material and sample process actuating mechanism, in which: the sample process consumptive material includes being arranged described
The one or more processing units of substrate and setting on the substrate on operating platform, each processing unit includes multiple
The cavity being independently arranged；Sample process actuating mechanism includes the first attachment base that can slide up and down to setting, for driving
It states first longitudinal direction mobile device that the first attachment base slides up and down, connect with first attachment base or first longitudinal direction mobile device
For being removably installed the liquid relief pipe connector of the pipette, for driving the pipette to carry out imbibition and drain movement
The first liquid relief driving device, sample process actuating mechanism be set to the mobile mechanism on and under the driving of the mobile mechanism
It can be moved along left and right directions, the front-rear direction of the digital pcr system；
The mobile mechanism, the sample process actuating mechanism, the nucleic acid amplification temperature control mechanism, the product signal harvester
Structure connect with the control mechanism respectively and is controlled by the control mechanism.
2. digital pcr system according to claim 1, it is characterised in that: the setting of the multiple cavity hatch upward.
3. digital pcr system according to claim 1, it is characterised in that: the multiple cavity is spaced apart and sets side by side
It sets, the axial line of each cavity extends respectively along the short transverse of the substrate.
4. digital pcr system according to claim 1, it is characterised in that: each processing unit respectively includes 6 ~ 20
Cavity.
5. digital pcr system according to claim 1, it is characterised in that: be also formed on the substrate for placing
State the support portion of pipette and/or the sampling probe.
6. digital pcr system according to claim 1, it is characterised in that: the temperature control mechanism further includes in cavity
Second heating component of liquid heating, second heating component includes heating coil；The sample process mechanism further includes magnetic
Body.
7. digital pcr system according to claim 6, it is characterised in that: the heating coil, the magnet are able to
Movably it is arranged.
8. digital pcr system according to claim 6 or 7, it is characterised in that: first heating component, the second heating
Component is respectively positioned below the operating platform, and the heating coil, the magnet are able to slide up and down to across the behaviour
Make platform.
9. digital pcr system according to claim 6 or 7, it is characterised in that: in each processing unit, have one or
Heat conducting element is provided on the corresponding substrate of multiple cavitys.
10. digital pcr system according to claim 9, it is characterised in that: the heat conducting element is elongated shape, until
It is at least partially located on the axis in the cavity and transverse to cavity.
11. digital pcr system according to claim 6 or 7, it is characterised in that: in each processing unit, have one or
Magnetic conductive component is provided on the corresponding substrate of multiple cavitys.
12. digital pcr system according to claim 11, it is characterised in that: the magnetic conductive component is elongated shape, until
It is at least partially located on the axis in the cavity and transverse to cavity.
13. digital pcr system according to claim 1, it is characterised in that: the sample process consumptive material further includes having examination
The reagent consumptive material of agent storage chamber, the reagent storage chamber are connected to a cavity in the multiple cavity by microchannel.
14. digital pcr system according to claim 13, it is characterised in that: the reagent consumptive material and the substrate are removable
It connects with unloading.
15. digital pcr system according to claim 14, it is characterised in that: interface is provided on the substrate, it is described
Reagent consumptive material includes shell, the intracorporal U-shaped Reagent Tube of the shell, the setting interface with the substrate on the housing is arranged in
A pair of of plug of grafting is matched, the inner cavity of the Reagent Tube constitutes the reagent storage chamber, the both ends of the Reagent Tube
It is connected separately with micro-pipe, two micro-pipes are each passed through the pair of plug setting, when the reagent consumptive material and the substrate
When connection, one inner cavity in two micro-pipes is constituted the bottom of the inner cavity of the Reagent Tube and a cavity
The microchannel of connection, another is the device that liquid can be driven to flow for connecting drive module, the drive module.
16. digital pcr system according to claim 1, it is characterised in that: the multiple cavities edge in each processing unit
The length direction of the digital pcr system be arranged side by side, the drop formation mechanism and sample process actuating mechanism edge
The digital pcr system width direction be arranged side by side.
17. according to claim 1 or digital pcr system described in 16, it is characterised in that: the drop formation mechanism includes can
Slide up and down to the second attachment base of the setting, oscillation drive being arranged on second attachment base, described for driving
Second longitudinal direction mobile device that second attachment base slides up and down, be arranged on the oscillation drive for removably pacifying
Fill the sampling probe connector of the sampling probe, for driving the sampling probe to carry out the second liquid relief driving of imbibition and drain movement
Device is additionally provided with drop receptacle mounting portion on the operating platform, is removably installed liquid by the drop receptacle mounting portion
Drip container.
18. digital pcr system according to claim 17, it is characterised in that: the mobile mechanism includes along the number
The X direction guiding rail that the length direction of PCR system extends, the mobile pedestal being slidably connected with the X direction guiding rail are arranged in the movement
On pedestal and along the Y-direction guide rail that the width direction of the digital pcr system extends, the sliding being slidably connected with the Y-direction guide rail
Seat is respectively used to drive the first motor driving device and the second motor driver of the mobile pedestal, sliding seat sliding, institute
State drop formation mechanism, the product signal acquisition module is separately positioned on the sliding seat.
19. digital pcr system according to claim 18, it is characterised in that: the sliding seat includes consolidating of being uprightly arranged
Determine block, the first sliding rail, the second sliding rail extended along up and down direction, first connection are respectively arranged on the fixed block
Seat, the second attachment base are arranged side by side, and the two is slidably connected with first sliding rail, the second sliding rail respectively.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821678881.2U CN209024540U (en) | 2018-10-17 | 2018-10-17 | A kind of digital pcr system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821678881.2U CN209024540U (en) | 2018-10-17 | 2018-10-17 | A kind of digital pcr system |
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| Publication Number | Publication Date |
|---|---|
| CN209024540U true CN209024540U (en) | 2019-06-25 |
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ID=66906615
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821678881.2U Active CN209024540U (en) | 2018-10-17 | 2018-10-17 | A kind of digital pcr system |
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| CN (1) | CN209024540U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113462556A (en) * | 2021-09-02 | 2021-10-01 | 德诺杰亿（北京）生物科技有限公司 | Heating assembly of nucleic acid extractor |
| CN113956968A (en) * | 2021-10-20 | 2022-01-21 | 西安天隆科技有限公司 | Liquid drop type digital PCR system and analysis method for realizing absolute quantification thereof |
-
2018
- 2018-10-17 CN CN201821678881.2U patent/CN209024540U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113462556A (en) * | 2021-09-02 | 2021-10-01 | 德诺杰亿（北京）生物科技有限公司 | Heating assembly of nucleic acid extractor |
| CN113462556B (en) * | 2021-09-02 | 2021-12-28 | 德诺杰亿（北京）生物科技有限公司 | Heating assembly of nucleic acid extractor |
| CN113956968A (en) * | 2021-10-20 | 2022-01-21 | 西安天隆科技有限公司 | Liquid drop type digital PCR system and analysis method for realizing absolute quantification thereof |
| CN113956968B (en) * | 2021-10-20 | 2023-11-14 | 西安天隆科技有限公司 | Liquid drop type digital PCR system and analysis method for realizing absolute quantification thereof |
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