CN209210821U - Droplet type digital pcr system - Google Patents

Abstract

The utility model discloses a kind of droplet type digital pcr systems, comprising: pedestal, mobile mechanism on the base is set, operating platform on the base is set, drop formation module, nucleic acid amplification temperature control module, product signal acquisition module and control module, operating platform is equipped with the drop receptacle mounting portion for being removably installed drop receptacle and the microchannel bracket for placing microchannel, drop formation actuating mechanism, product signal acquisition module is respectively arranged in mobile mechanism and can be along the left and right directions of droplet type digital pcr system under the driving of mobile mechanism, front-rear direction movement, moving drive mechanism, drop formation actuating mechanism, nucleic acid amplification temperature control module, product signal acquisition module connect with control module respectively and is controlled by control module.The droplet type digital pcr system structure is reasonable, easy to use, and avoids manual operation, reduces work time-consuming, improves work efficiency.

Description

Droplet type digital pcr system

Technical field

The utility model relates to a kind of digital pcr systems, especially droplet type digital pcr system.

Background technique

Polymerase chain (PCR) reaction technology is modern biology one of the most important instruments, it is widely used in medicine Diagnosis, Personalized medicine, Food Inspection, genetically modified organism detection, Causal Agent Identification, immunoassay, forensic science etc..And Had more than traditional qPCR as the latest generation of round pcr based on the digital pcr (dPCR) that microflow control technique development generates Small reaction volume, faster reaction speed, lower system noise and higher sensitivity.

Droplet type digital pcr system carries out droplet processing to sample before traditional PCR amplification, i.e., will contain nucleic acid point The reaction system of son is divided into the droplets of thousands of a nanoliter levels, wherein each droplet or nucleic acid target molecule to be checked is free of, or Contain one to several nucleic acid target molecules to be checked.After PCR amplification, each droplet is detected one by one, there is fluorescence signal Droplet interpretation is 1, and the droplet interpretation of fluorescence signal is not 0, according to Poisson distribution principle and the number and ratio of positive droplet The starting copy number or concentration of you can get it target molecule.Microdrop technique is more inexpensive by digital pcr, and more practical.

Droplet type digital pcr system generally includes drop formation device, nucleic acid amplification temperature regulating device and product signal and adopts Collection and analytical equipment.In the prior art, although the existing document report about each specific device, have no about automation, The document report of integral type droplet type digital pcr system.

Currently, the measuring means such as blood routine, cytology, pathology and immunology are towards automation, integration, standardization Direction develop, but due to Molecular Detection self-technique complexity, the automation from sample to result realize during there is Many insoluble technical problems.Such as PCR reaction before generally require by hand sample-adding operation, PCR need after reaction by Reactor taking-up is transferred to pertinent instruments and carries out fluorescence signal detection, then carries out calculating analysis, manual operation be easy to cause mistake Difference and generation cross contamination, and manual operation takes long time, process is cumbersome, influences the accuracy of result.

Summary of the invention

The purpose of this utility model is to provide a kind of droplet type digital pcr systems of automation, and the system structure is reasonable, It is easy to use.

In order to achieve the above objectives, a kind of technical solution that the utility model uses is:

A kind of droplet type digital pcr system, the droplet type digital pcr system includes: pedestal, is arranged in the pedestal On mobile mechanism, the operating platform on the pedestal is set, drop formation module, nucleic acid amplification temperature control module, product letter Number acquisition module and control module；

The drop formation module includes microchannel, drop receptacle, drop formation actuating mechanism, and the drop formation is dynamic As mechanism include fixed bracket, the oscillation drive that can be slidably arranged in along up and down direction on the fixed bracket, Longitudinal moving device for driving the oscillation drive to slide up and down is arranged on the oscillation drive and is used for It is removably installed the connector of the microchannel, for driving the microchannel to carry out the fluid driving of imbibition and drain movement Device；

The operating platform is equipped with and is removably installed the drop receptacle mounting portion of the drop receptacle and for placing The microchannel bracket of the microchannel；

The nucleic acid amplification temperature control module includes the heating component below the drop receptacle mounting portion；

The drop formation actuating mechanism, the product signal acquisition module be respectively arranged in the mobile mechanism and It can be moved along left and right directions, the front-rear direction of the droplet type digital pcr system under the driving of the mobile mechanism；

The mobile mechanism, drop formation actuating mechanism, the nucleic acid amplification temperature control module, product signal acquire mould Block connect with the control module respectively and is controlled by the control module.

Some preferred embodiments according to the present utility model are additionally provided with the sample for placing sample cell on the operating platform Quality control bracket or sample treatment modules with sample storage chamber.

Some preferred embodiments according to the present utility model, the droplet type digital pcr system further include described for making The separation module that is separated with the microchannel of connector and receiving separate after microchannel or waste liquid waste collection component, The separation module includes being arranged on the oscillation drive to be used to that the microchannel to be made to separate with the connector Separation driving unit and the withdraw of the needle bracket for microchannel fixed in separation that is arranged on the operating platform, it is described useless Object collecting part is located at the lower section of the operating platform and withdraw of the needle bracket.

Some more preferable modes according to the present utility model are additionally provided with for placing sample cell on the operating platform Sample tube rack or sample treatment modules with sample storage chamber, the droplet type digital pcr system further includes for making The separation module that the connector is separated with the microchannel, the separation module include being arranged in the vibratory drive Separation driving unit for separating the microchannel with the connector on device and it is arranged in the operating platform The withdraw of the needle bracket for microchannel fixed in separation, at the drop receptacle mounting portion, the sample tube rack or sample Reason module, the microchannel bracket, the withdraw of the needle bracket are set gradually along the length direction of the operating platform.

Some preferred embodiments according to the present utility model, the droplet type digital pcr system further include being arranged described Pedestal on the reservoir part for storage driving oil, the driving that is connected to the reservoir part with the fluid drive apparatus Oil-piping and the pump being arranged on the driving oil-piping.

Further, the droplet type digital pcr system further includes being arranged on the fixed bracket and the driving Oil-piping connection pump nozzle, the centerline parallel of the center line of the pump nozzle, the microchannel, the pump nozzle be used for Injection driving is oily in the drop receptacle or draws liquid from the drop receptacle.

Some preferred embodiments according to the present utility model, the oscillation drive include rotating electric machine, rotary shaft, described The output end of rotating electric machine is connected with the rotary shaft, and the connector is along the direction perpendicular with the rotating shaft axis It is fixedly connected in the rotary shaft, multiple connectors is equipped in the rotary shaft.

Some preferred embodiments according to the present utility model, the drop receptacle are the reaction utensil or core that top has opening Piece, the chip include the chip body with drop storage chamber, the inlet being arranged on the chip body, upright setting The accommodating cavity that is connected on the chip body and with the inlet, the leakage fluid dram being arranged on the chip body, by institute State the first passage that inlet is connected to the drop storage chamber, be connected to the leakage fluid dram with the drop storage chamber second Channel.

Some preferred embodiments according to the present utility model, the nucleic acid amplification temperature control module further include being slidably arranged in institute The heating cover on operating platform stated, the heating cover have first position and the second position, described to add when being in first position Heat lid is pressed on the drop receptacle, is heated to the drop receptacle；When in the second position, the heating cover and institute State drop receptacle separation.

Some preferred embodiments according to the present utility model, the mobile mechanism include along the droplet type digital pcr system Length direction extend X direction guiding rail, the mobile pedestal being slidably connected with the X direction guiding rail, be arranged on the mobile pedestal And the Y-direction guide rail extended along the width direction of the droplet type digital pcr system, the sliding being slidably connected with the Y-direction guide rail Seat is respectively used to drive the first motor driving device and the second motor driver of the mobile pedestal, sliding seat sliding, institute The drop formation actuating mechanism stated, the product signal acquisition module are separately positioned on the sliding seat.

Due to the application of the above technical scheme, the utility model has the advantage that compared with prior art

The droplet type digital pcr system collection droplet formation of the utility model expands, is detected on one, realizes raw from drop At the automation control for arriving result detection again to PCR reaction.The structure design of the utility model is reasonable, easy to use, can effectively mention High working efficiency and reduction operating error.

Detailed description of the invention

Fig. 1 is the overall structure diagram of the droplet type digital pcr system of embodiment；

Fig. 2 is the structural schematic diagram of the sample operation platform of the droplet type digital pcr system of embodiment；

Fig. 3 is the schematic front view of the sample operation platform of the droplet type digital pcr system of embodiment；

Fig. 4 is the schematic front view of the drop formation actuating mechanism of the droplet type digital pcr system of embodiment；

Fig. 5 is the right side view of the drop formation actuating mechanism of the droplet type digital pcr system of embodiment；

Fig. 6 is the structural schematic diagram of drop receptacle in embodiment；

Wherein: 1, pedestal；2, operating platform；21, drop receptacle mounting portion；22, sample tube rack；23, microchannel bracket； 24, waste collection component；25, withdraw of the needle bracket；3, drop formation module；30, drop formation actuating mechanism；300, microchannel； 310, drop receptacle；311, drop chip；3110, chip body；3111, storage chamber；3112, accommodating cavity；3113, inlet； 3114, leakage fluid dram；3115, first passage；3116, second channel；3117, drain diversion pipe；320, fixed bracket；330, it vibrates Driving device；331, rotating electric machine；332, rotary shaft；340, longitudinal moving device；341, vertical drive motor；350, it connects Head；360, fluid drive apparatus；361, oily spindle motor is pushed away；362, silk noil thick stick is pushed away；371, driving unit is separated；380, it drives Oil-piping；390, pump nozzle；4, nucleic acid amplification temperature control module；40, heating component；41, heating/refrigerating piece；42, thermal component； 43, heating cover；44, heating cover fixing clamp；45, heating cover guide rail；5, product signal acquisition module；6, control module；7, it stores Component；71, it pumps；9, mobile mechanism；91, X direction guiding rail；92, mobile pedestal；93, Y-direction guide rail；94 sliding seats 95, first motor are driven Dynamic device；951, X-axis drag chain；96, the second motor driver；961, Y-axis drag chain.

Specific embodiment

The technical solution of the utility model is further elaborated with specific embodiment with reference to the accompanying drawing.

Firstly, in conjunction with the embodiments and attached drawing, the specific knot for the microlayer model generating means that the utility model uses is described in detail Structure and working principle.It should be appreciated that specific embodiment described herein is not used to limit only to explain the utility model The utility model.

Front-rear direction described in the utility model refers to the X-direction in Fig. 1, and left and right directions refers to the Y-axis side in Fig. 1 To up and down direction refers to the Z-direction in Fig. 1；

The utility model provides a kind of droplet type digital pcr system, the droplet type digital pcr system collection droplet formation, It expands, be detected on one, constituting substantially includes pedestal, and mobile mechanism on the base is arranged, operation on the base is arranged Platform, drop formation module, nucleic acid amplification temperature control module, product signal acquisition module and control module；The utility model Droplet type digital pcr system is realized from the automation control of the detection after drop formation, pcr amplification reaction and reaction, is not only kept away Exempt from manual operation, reduced work time-consuming, also improves the accuracy of result.

As shown in Figure 1, the shifting on pedestal 1 is arranged in including pedestal 1 in a specific embodiment of the utility model The operating platform 2 on pedestal 1, drop formation module 3, nucleic acid amplification temperature control module 4, product signal acquisition is arranged in motivation structure 9 Module 5 and control module 6.

As shown in Fig. 2, Fig. 4, Fig. 5, drop formation module 3 includes microchannel 300, drop receptacle 310, drop formation movement Mechanism 30.Drop formation actuating mechanism 30 includes fixing bracket 320, capable of being slidably arranged in fixed bracket along up and down direction On for driving microchannel 300 to do oscillation drive 330 of reciprocating vibration, for driving about 330 oscillation drive The longitudinal moving device 340 of sliding, be arranged on oscillation drive 330 for being removably installed the microchannel 300 Connector 350, for drive microchannel 300 carry out imbibition and drain movement fluid drive apparatus 360.Drop formation module 3 drop formation principle is: oily phase is injected in drop receptacle 310, by the lower end of microchannel 300 be inserted into oil phase liquid face it Under, drive sample solution to flow through microchannel 300 while using oscillation drive to microchannel 300 using fluid drive apparatus 36 It is vibrated, the drop generated from microchannel 300 enters in oily phase.Wherein for drawing and releasing the fluid drive apparatus of liquid 360 are not particularly limited, and can be driving device commonly used in the art, such as syringe pump, and the fluid in the present embodiment drives dress Setting 360 is to utilize to push away oily spindle motor 361 and push away the cooperation of silk noil thick stick 362 to complete absorption and release liquid.For driving microchannel The oscillation drive of 300 vibrations can preferably include to can be realized the reciprocal vibration of microchannel 300 short stroke high speed there are many form Dynamic vibratory equipment, the reciprocating motion can be up and down reciprocatingly vibration, the vibration of left and right reciprocating linear or rotate left and right reciprocal Movement.Vibratory equipment is specifically such as electromagnet type, piezoelectric ceramic type or mechanical eccentric is wheeled, oscillating cylinder, rotating electromagnet Equipment.In the present embodiment oscillation drive 330 include rotating electric machine 331, rotary shaft 332, the output end of rotating electric machine 331 with Rotary shaft 332 is connected, and connector 350 is fixedly connected on rotary shaft 332 along the direction with 332 axis perpendicular of rotary shaft On, multiple connectors 350 are equipped in rotary shaft 332, rotating electric machine 331 drives rotary shaft 332 reciprocating, rotation Shaft 332 drives connector 350 and the microchannel 300 being connected with connector 350 reciprocating in turn, oscillation drive 330 further include the connecting bracket (unmarked) that rotating electric machine 331 is connected with longitudinal moving device 340.It is vibrated for driving The longitudinal moving device 340 that driving device 330 slides up and down is not particularly limited, can be real using screw structure or rack structure It is existing, herein without repeating.

As shown in Figure 2 and Figure 3, operating platform 2 is equipped with the drop receptacle mounting portion for being removably installed drop receptacle 310 21 and the microchannel bracket 23 for placing microchannel 300, the sample tube rack 22 for placing sample cell or have sample storage The sample treatment modules (not shown) of chamber is deposited, the sample treatment modules with sample storage chamber are specifically, for example, to have multiple cavities Device, cracking, combination, cleaning, elution and the core that will be extracted of sample (such as nucleic acid) can be completed on this device Acid, which quantify with PCR reagent, the sequence of operations such as mixes.

As shown in figs. 3 and 5, droplet type digital pcr system further includes for separating connector 350 with microchannel 300 Separation module 200 and the waste collection component 24 for receiving microchannel 300 or waste liquid after separation, separation module include that setting is being shaken Separation driving unit 371 for keeping microchannel 300 isolated with connector 350 and setting in dynamic driving device 330 is described The withdraw of the needle bracket 25 for microchannel 300 fixed in separation on operating platform 2 in the present embodiment, separates driving unit 371 It is arranged in connecting bracket；A preferred embodiment in the present embodiment, drop receptacle mounting portion 21, sample tube rack 22 or sample Processing module, microchannel bracket 23, withdraw of the needle bracket 25 are set gradually along the length direction of the operating platform 2, in this reality Another preferred embodiment in example is applied, for convenient for collecting the microchannel 300 after use, waste collection component 24 is located at operating platform 2 With the lower section of withdraw of the needle bracket 25；When microchannel 300 after needing to use is unloaded from drop formation module 3, pass through moving machine Structure drives drop formation module 3 to move to 25 top of withdraw of the needle bracket, continues operation mobile mechanism for microchannel 300 and is stuck in withdraw of the needle bracket On 25, separation driving unit 371 drives microchannel 300 to return from connector 350, and microchannel 300 falls to waste collection portion In part 24.

As shown in Figures 3 to 5, nucleic acid amplification temperature control module 4 includes the heating group positioned at 21 lower section of drop receptacle mounting portion Part 40, the heating cover 43 being slidably arranged on operating platform 2, heating cover 43 have first position and the second position, in the (not shown) when one position, heating cover 43 are pressed on drop receptacle 110, are heated to drop receptacle 310；It is in When the second position (as shown in Figure 3), heating cover 43 is separated with drop receptacle 310, it is preferable that nucleic acid amplification temperature control module 4 may be used also To include heating cover fixing clamp 44, heating cover guide rail 45, heating cover fixing clamp 44 is for being pressed on drop receptacle for heating cover 43 On 110, heating cover 43 is slidably connected with 45 phase of heating cover guide rail, heating cover 43 can along heating cover guide rail 45 in first position and Switch between the second position.Heating component 40 specifically includes heating/refrigerating piece 41, thermal component 42 etc., and heating/refrigerating piece 41 is used for Suitable reaction temperature is provided for PCR drop, selects semiconductor chip as heating/refrigerating piece in the present embodiment.

Drop receptacle 310 is the reaction utensil that can be top and have opening, can also be other forms, such as drop chip 311.As shown in fig. 6, a representative drop chip 311 includes the chip body 3110 with drop storage chamber 3111, sets The accommodating cavity for setting the inlet 3113 on chip body, being uprightly arranged on chip body and be connected to inlet 3113 3112, the leakage fluid dram 3114 being arranged on chip body, the row for being uprightly arranged on chip body and being connected to leakage fluid dram 3114 Liquid diversion pipe 3117, the first passage 3115 that inlet is connected to the drop storage chamber, by leakage fluid dram and drop storage chamber The second channel 3116 of connection, it is preferable that downward tilt angle is arranged at 3112 bottom of drop accommodating cavity, such that in liquid The drop that drop accommodating cavity 3112 generates can the trend gathered of oriented 3113 position of inlet, drop of being more convenient for enters the storage of drop In chamber 3111.

In this example, the course of work of drop is generated in drop chip 311: first from accommodating cavity 311, first passage 3115 To storage chamber 3111, then it is full of oil phase liquid into two channels 3116, leakage fluid dram 3114, then microchannel 300 is inserted into and is filled Drop formation is carried out in 3112 container of accommodating cavity of full oil phase, the droplet falls free of generation simultaneously falls in 3112 bottom of accommodating cavity, Since downward tilt angle arranged at 3112 bottom of accommodating cavity, drop can the trend gathered of oriented 3113 position of inlet；Drop is raw At after the completion, drop generates the microchannel 300 of actuating mechanism 30 with second channel 3116, by generating negative pressure for accommodating cavity Drop in 3112 is drawn into storage chamber 3111, and fan-shaped 3111 bottom of storage chamber that is laid in (in this example, cooperates respective negative The speed of pressure can produce different tiling effects).The drop chip structure is taken, it is close compared with the reaction utensil for taking opening Envelope property is more preferable, using more convenient, can also realize multilayer tiling of the drop in drop storage chamber, greatly improve detection flux, Meet the requirement of high-throughput liquid drop analysis in clinically automation and unit area.

As shown in Figure 1, droplet type digital pcr system further includes the reservoir part for storage driving oil on pedestal 1 7, the driving oil-piping 380 being connected to reservoir part 7 with fluid drive apparatus 360 and setting are on the driving oil-piping 380 Pump 72.

As shown in figure 4, droplet type digital pcr system further includes that setting connects on fixed bracket 320 with driving oil-piping 380 Logical pump nozzle 370, the centerline parallel of the center line of the pump nozzle 370, microchannel 300, pump nozzle 370 are used for drop Injection driving is oily in container 310 or draws liquid from drop receptacle 310.

In the present embodiment, mobile mechanism 9 includes the X direction guiding rail 91 extended along the length direction of droplet type digital pcr system, The mobile pedestal 92 being slidably connected with X direction guiding rail is arranged on mobile pedestal 92 and along the width side of droplet type digital pcr system To the Y-direction guide rail 93 of extension, the sliding seat 94 being slidably connected with Y-direction guide rail is respectively used to drive the mobile pedestal 92, sliding The first motor driving device 95 and the second motor driver 96 of 94 sliding of seat, first motor driving device 95 include driving electricity Machine (not shown) and X-axis drag chain 951, the second motor driver 96 include driving motor (not shown) and Y-axis drag chain 961.Liquid Drop generation actuating mechanism 30, product signal acquisition module point 5 She Zhi not be on sliding seats 94, and drop formation actuating mechanism 30 is whole Body, product signal acquisition module 5 can be moved with front-rear direction in left-right direction under the driving of mobile mechanism 9.Product signal This field conventional arrangement mode specifically can be used in acquisition module 5, including camera, optical fiber, exciting light turbin generator etc..

It include mobile mechanism 9, drop formation actuating mechanism 30, nucleic acid amplification temperature control module 4, product signal in the present embodiment Acquisition module 5 connect with the electrical connection of control module 6 and/or signal respectively and is controlled by control module 6.Control module 6 includes control Device processed, signal output interface etc..Signal output interface is for connecting display device, control panel etc..In embodiment, pedestal 1 exists The rear of product signal acquisition module 5 is equipped with a upright bracket, and control module 6 is arranged on the bracket.

The course of work of the digital pcr system of the present embodiment is as follows:

Microchannel 300 is placed on microchannel bracket 23 first, the sample cell equipped with sample is placed into sample tube rack On 22, and is set in operation sequence and generate amount of droplets and relevant parameter；Control module 6 is controlled by relevant instruction information Mobile mechanism 9 drives drop formation actuating mechanism 30 to be moved to 310 position of drop receptacle, in the effect of longitudinal driving device 340 Under, pump nozzle 390 opens pump 72 and is injected into formula oil in drop receptacle 310 from reservoir part 7 close to drop receptacle 310, To preset height；Pass through the mobile microchannel bracket of control mobile mechanism 9 driving drop formation actuating mechanism 30 after adding formula oil It at 23, combines the connector 350 in drop formation actuating mechanism 30 with microchannel 300, drives connector 350 upward again later It is mobile to reset, so that microchannel 300 is left microchannel bracket 23, completes the installation of microchannel 300, pass through fluid drive apparatus 360 Effect, release power oil full of microchannel 300 until being discharged in a small amount of power oil to lower section waste collection component 24, after the completion Drop formation actuating mechanism 30 is driven to reset；Then driving drop formation actuating mechanism 30 moves at sample tube rack 22, will be micro- Pipeline 300 is inserted into sample, is sucked the sample into microchannel 300 using fluid drive apparatus 360, after the completion of inhaling sample, is driven Dynamic drop formation actuating mechanism 30 resets upwards, and microchannel 300 leaves sample cell, moves at drop receptacle 310, and by microchannel 300 are inserted into the formula oil of drop receptacle 310, and fluid drive apparatus 360 releases sample liquids from microchannel 300, vibration Dynamic driving device 330, which generates vibration, keeps the opening of microchannel 300 of reciprocating vibration in formula oil, meanwhile, mobile mechanism 9 drives drop Generate actuating mechanism integrally moves it is mobile by preset track so that microchannel 300 is while doing of reciprocating vibration, also along presetting Track it is mobile, so that the drop generated be made to be evenly distributed in drop receptacle 310；After drop formation, drop is driven It generates actuating mechanism 30 to move at withdraw of the needle bracket 25, in the case where separation driving unit 371 is acted on by microchannel 300 from connector 350 On return and fall into waste collection component 24, drive drop formation actuating mechanism 30 to reset after the completion；Later, by heating cover 43 It is moved to 310 top of drop receptacle, and utilizes 44 clamping of heating cover fixing clamp, nucleic acid amplification temperature control module 4 is begun to warm up, power supply Driving semiconductor heating/refrigerating piece is heated up and is cooled down, and is completed a certain number of circulations by program is pre-designed, is held drop Nucleic acid amplification reaction, while 43 continuous heating of heating cover are carried out in device 310；After the completion of heat cycles, releases heating cover 43 and fixes, Heating cover 43 is moved into the second position, driving product signal acquisition module 5 is moved at drop receptacle 310, by controlling product Signal acquisition module 5 is observed, shoots photo, is sent to control module 6 and carries out data processing and analysis.

The above embodiments are only for explaining the technical ideas and features of the present invention, and its object is to allow be familiar with technique Personage can understand the content of the utility model and be implemented, do not limit the protection scope of the present invention, All equivalent change or modifications according to made by the spirit of the present invention essence, should all cover in the protection scope of the utility model It is interior.

Claims (10)

1. a kind of droplet type digital pcr system, it is characterised in that: the droplet type digital pcr system includes: pedestal, setting The operating platform on the pedestal, drop formation module, nucleic acid amplification temperature control mould is arranged in mobile mechanism on the pedestal Block, product signal acquisition module and control module；

The drop formation module includes microchannel, drop receptacle, drop formation actuating mechanism, and the drop formation acts machine Structure includes fixed bracket, the oscillation drive that can be slidably arranged on the fixed bracket along up and down direction, is used for The longitudinal moving device that drives the oscillation drive to slide up and down, be arranged on the oscillation drive for removable The connector of the microchannel is installed with unloading, for driving the microchannel to carry out the fluid driving dress of imbibition and drain movement It sets；

The operating platform is described equipped with the drop receptacle mounting portion for being removably installed the drop receptacle and for placing Microchannel microchannel bracket；

The nucleic acid amplification temperature control module includes the heating component below the drop receptacle mounting portion；

The drop formation actuating mechanism, the product signal acquisition module are respectively arranged in the mobile mechanism and described It can be moved along left and right directions, the front-rear direction of the droplet type digital pcr system under the driving of mobile mechanism；

The mobile mechanism, drop formation actuating mechanism, the nucleic acid amplification temperature control module, product signal acquisition module point It does not connect with the control module and is controlled by the control module.

2. droplet type digital pcr system according to claim 1, it is characterised in that: be additionally provided on the operating platform For placing the sample tube rack of sample cell or the sample treatment modules with sample storage chamber.

3. droplet type digital pcr system according to claim 1, it is characterised in that: the droplet type digital pcr system It further include the microchannel or useless after separation module and receiving for separating the connector with the microchannel separates The waste collection component of liquid, the separation module include being arranged on the oscillation drive to be used to make the microchannel The separation driving unit that is separated with the connector and be arranged on the operating platform for microchannel fixed when separating Withdraw of the needle bracket, the waste collection component is located at the lower section of the operating platform and withdraw of the needle bracket.

4. droplet type digital pcr system according to claim 1, it is characterised in that: be additionally provided on the operating platform For placing the sample tube rack of sample cell or the sample treatment modules with sample storage chamber, the droplet type digital pcr System further includes the separation module for separating the connector with the microchannel, and the separation module includes setting Set the separation driving unit for separating the microchannel with the connector on the oscillation drive and setting In the withdraw of the needle bracket for microchannel fixed in separation on the operating platform, the drop receptacle mounting portion, the sample Quality control bracket or sample treatment modules, the microchannel bracket, the withdraw of the needle bracket along the operating platform length Direction is set gradually.

5. droplet type digital pcr system according to claim 1, it is characterised in that: the droplet type digital pcr system It further include that driving on the pedestal for the reservoir part of storage driving oil, by the reservoir part and the fluid is set The driving oil-piping of dynamic device connection and the pump being arranged on the driving oil-piping.

6. droplet type digital pcr system according to claim 5, it is characterised in that: the droplet type digital pcr system It further include that the pump nozzle being connected on the fixed bracket with the driving oil-piping, the center line of the pump nozzle, institute are set State the centerline parallel of microchannel, the pump nozzle is used for into the drop receptacle injection driving oil or from the drop receptacle Middle absorption liquid.

7. droplet type digital pcr system according to claim 1, it is characterised in that: the oscillation drive includes rotation Rotating motor, rotary shaft, the output end of the rotating electric machine are connected with the rotary shaft, the connector along with the rotation The direction of axis axis perpendicular is fixedly connected in the rotary shaft, and multiple connectors are equipped in the rotary shaft.

8. droplet type digital pcr system according to claim 1, it is characterised in that: the drop receptacle is top tool There are the reaction utensil or chip of opening, the chip includes the chip body with drop storage chamber, is arranged in the chip body On inlet, uprightly be arranged on the chip body and be connected to the inlet accommodating cavity, setting in the chip Leakage fluid dram on ontology, the first passage for being connected to the inlet with the drop storage chamber, by the leakage fluid dram with it is described The second channel of drop storage chamber connection.

9. droplet type digital pcr system according to claim 1, it is characterised in that: the nucleic acid amplification temperature control module is also Including the heating cover being slidably arranged on the operating platform, the heating cover has first position and the second position, place When first position, the heating cover is pressed on the drop receptacle, is heated to the drop receptacle；In second When setting, the heating cover is separated with the drop receptacle.

10. droplet type digital pcr system according to claim 1, it is characterised in that: the mobile mechanism includes along described The X direction guiding rail that the length direction of droplet type digital pcr system extends, the mobile pedestal being slidably connected with the X direction guiding rail, setting On the mobile pedestal and along the Y-direction guide rail that the width direction of the droplet type digital pcr system extends, led with the Y-direction The sliding seat that rail is slidably connected is respectively used to drive the mobile pedestal, the first motor driving device of sliding seat sliding and the Two motor drivers, the drop formation actuating mechanism, the product signal acquisition module are separately positioned on the cunning On dynamic seat.