CN209193947U - Sample process consumptive material, sample processing apparatus and digital pcr system - Google Patents

Abstract

The utility model discloses a kind of sample processing apparatus and the digital pcr system including the processing unit, sample processing apparatus includes sample process consumptive material, the drive module and control module that connect with sample process consumptive material, sample process consumptive material includes substrate, multiple extraction chambers, sample chamber, sample chamber is arranged in and extracts the transfering channel for being used to for the two being connected between chamber, with the reagent consumptive material of reagent storage chamber, multiple extraction chambers are connected in bottom by microchannel, and reagent storage chamber is connected to sample chamber by microchannel.The sample processing apparatus of the utility model can have the multiple functions such as mixing nucleic acid extraction and reagent, suitable for obtaining the sample for being directly used in digital pcr detection, and the apparatus structure is simple, compact, small in size, can modularized design, be suitable for building unitary drop formula digital pcr system.

Description

Sample process consumptive material, sample processing apparatus and digital pcr system

Technical field

The utility model relates to field of molecular detection, and in particular to the sample process dress suitable for digital pcr detection It sets and the digital pcr system including the sample processing apparatus, especially drop formula digital pcr system.

Background technique

With the transformation of medical model and the continuous development of personalized medicine, there is an urgent need to quick, accurate for medical test circle Detection means, wherein Molecular Detection have unique advantage.

Currently, molecular detection technology mainly has making nucleic acid molecular hybridization, polymerase chain reaction (PCR) and biochip technology Deng.Molecular Detection product is mainly used in the detection and physical examination of the clinical departments such as tumour, infection, heredity, Prenatal Screening The heart, technical service center, third party testing agency and microbial rapid detection market etc..

As the important technical of Molecular Detection, round pcr can qualitatively and quantitatively detect target nucleic acid molecules, Under the application demand background that low abundance detection, rare mutation detection etc. increasingly increase, digital pcr is absolute as a kind of nucleic acid molecules Quantitative technique, a quantitative fluorescent PCR reaction system is assigned in a large amount of small reactors by it, in each microreactor Target nucleic acid molecules comprising one or more copies carry out " unimolecule template PCR amplifications ", after amplification, by the positive The number and statistical method of reaction member calculate the copy number of target gene in original sample, and digital pcr need not rely on reference substance Accurately absolute quantitation detection can be carried out with standard curve.

Currently, the measuring means such as blood routine, cytology, pathology and immunology are towards automation, integration, standardization Direction develop, but due to Molecular Detection self-technique complexity, the automation from sample to result realize during there is Many insoluble technical problems.It is single that the preceding acquisition of sample of nucleic acid and the pre-treatment step of sample of nucleic acid are reacted with regard to digital pcr For, traditional approach requires more manual operations to participate in, and the degree of automation is low, and to the more demanding of use condition, The operation equipment for having profession is asked just to can be carried out.In order to solve these problems, the prior art is it has been suggested that automatic nucleic acid extraction Device, the device etc. that automatic nucleic acid extraction, amplification, detection are integrated.However, these apparatus structures are typically more complicated, and not Enough compact, volume is big, user's operation is complicated, is unsuitable for constructing unitary drop formula digital pcr system.

Summary of the invention

One of the purpose of this utility model is to provide compact-sized one kind, modularization, automation sample processing apparatus.

The utility model simultaneously also provide it is a kind of integrate sample process, drop formation, amplification and detect number Word PCR system, the system structure is compact, equipment volume is small, is arranged simple, easy to use.

In order to achieve the above objectives, a kind of technical solution that the utility model uses is:

A kind of sample process consumptive material comprising substrate, setting one or more processing units on base material, each processing are single Member include multiple extraction chambers, setting sample chamber on base material, be arranged in sample chamber and extraction chamber between for the two to be connected Logical transfering channel, the reagent consumptive material with reagent storage chamber, multiple extraction chambers pass through microchannel in bottom and are connected to, reagent storage Chamber with sample chamber be connected to by microchannel.

According to the utility model, when each microchannel is preferably disposed to when without outer power drive or driving force is lower than setting value Liquid will not be moved to the channel of another chamber from a chamber.The aperture of microchannel is, for example, 60 microns ~ 100 microns, excellent It is selected as 70 ~ 100 microns.

Preferably, the setting of the opening of chamber, sample chamber and reagent storage chamber respectively upward is extracted.

A specific aspect according to the present utility model, sample process consumptive material further include the valve set on extraction chamber opening, Valve is to close extraction chamber or extraction chamber is connected with atmosphere or the external component extracted other than chamber.Valve specifically can be such as two Port valve.

A preferred aspect according to the present utility model in each processing unit, has and one or more extracts the corresponding base of chambers Heat conducting element and/or magnetic conductive component are provided on material.It is preferred that being provided with heat conducting element and magnetic conductive component simultaneously.

Above-mentioned heat conducting element can cooperate with heating coil etc., improve to the heating effect and rate for extracting intracavity liquid. A specific aspect according to the present utility model, heat conducting element is preferably elongated shape, is at least partially situated at and extracts in chamber And transverse to the axis for extracting chamber.The heat conducting element of the setting can also play the effect of flow-disturbing, be conducive to the extraction intraluminal fluid The mixing of body.

Above-mentioned magnetic conductive component can be conducted with magnet contact, in some applications when such as nucleic acid separation and Extraction, Nucleic acid separation can be realized in conjunction with magnetic bead is used.A specific aspect according to the present utility model, magnetic conductive component is elongated shape Shape is at least partially situated at and extracts in chamber and transverse to the axis for extracting chamber.The magnetic conductive component of the setting can also be played and be disturbed The effect of stream is conducive to the mixing of the extraction intracavity liquid.

Preferably, sample process consumptive material further include be at least partially situated in sample chamber and transverse to sample chamber axle center The tabulator bars of line setting.

A preferred aspect according to the present utility model, multiple extraction chambers are spaced apart, and extract the axial line of chamber, sample chamber Extend respectively along the short transverse of substrate, the axial line of transfering channel transverse to the axial line for extracting chamber, sample chamber.

One according to the present utility model specific and preferred aspect, multiple extraction chambers of each processing unit along substrate Length direction is set gradually, and the both ends of transfering channel are connected to sample chamber, near the extraction chamber of sample chamber respectively.

Preferably, transfering channel includes the first passage and second channel being connected in one end, wherein first passage Internal diameter be greater than second channel, the other end of first passage with extraction chamber be connected to, the other end of second channel and sample chamber Connection.

A specific aspect according to the present utility model, substrate include being formed with multiple first base material portions for extracting chamber, shape At the elongated interconnecting piece that the second base part for having sample chamber, both ends are connect with first base material portion and the second base part respectively, transfer Channel include along elongated interconnecting piece length direction extend be set to elongated interconnecting piece in first passage.

Preferably, second channel is formed in the second base part.

Preferably, the reagent consumptive material and substrate are mutually detachably connected.

One according to the present utility model specific and preferred aspect: being provided with interface on substrate, reagent consumptive material include shell, The intracorporal U-shaped Reagent Tube of shell is set, a pair of of the plug for matching grafting on shell with the interface of substrate is set, Reagent Tube The reagent storage chamber that inner cavity is constituted, the both ends of Reagent Tube are connected separately with micro-pipe, and two micro-pipes are each passed through a pair of of plug setting, When reagent consumptive material is connect with substrate, one inner cavity in two micro-pipes constitute by the inner cavity of Reagent Tube and sample chamber bottom The microchannel that portion connects, for connecting drive module or atmosphere, the drive module is that liquid can be driven to flow for another Device.

A kind of embodiment according to the present utility model, the sample process consumptive material further include one or more sample process Required reagent, reagent be packaged in selected from it is multiple extraction chambers, reagent storage chamber cavity in.

One aspect according to the present utility model, sample process consumptive material are that PCR detects sample process consumptive material, each processing Unit includes 6 or more extraction chambers, and sample process consumptive material further includes set on the valve for extracting chamber opening, and valve is extracted to close Extraction chamber is connected with atmosphere or the external component extracted other than chamber in chamber, and in each processing unit, one or more extracts chambers pair The magnetic conductive component of heat conducting element and selectivity is provided on the substrate answered.Preferably, magnetic conductive component is set.

In some embodiments, it is provided with and leads on the multiple corresponding substrate of extraction chamber extracted in chamber Thermal element, is provided with magnetic conductive component on another adjacent corresponding substrate of extraction chamber, and heat conducting element, magnetic conductive component are transverse to mentioning The axial line of chamber is taken to be arranged and be respectively provided with exposed contact portion.

In some embodiments, sample process consumptive material further includes extracting core for substance needed for extracting nucleic acid The required substance of acid includes cleaning solution, cell pyrolysis liquid, enzyme, eluent, magnetic bead, and wherein cleaning solution, eluent, magnetic bead seal respectively Loaded in different extraction chambers, cell pyrolysis liquid is packaged in identical extraction chamber from enzyme or is packaged in different extraction chambers.

Another scheme that the utility model is taken is: a kind of sample processing apparatus comprising one or more originally practical Novel above-mentioned sample process consumptive material, the drive module and control module connecting with sample process consumptive material, drive module are used for Driving liquid flows in each chamber of sample process consumptive material or channel, and drive module connect with control module and by control module control System.

Preferably, sample processing apparatus further includes heating module, and heating module includes heating coil, heating module and control Molding block is connected and is controlled by control module.What heating module preferably can be movably arranged.

Preferably, sample processing apparatus further includes set on the magnet extracted outside chamber.Using magnet and magnetic bead, it can be achieved that core Acid separation.What magnet preferably can be movably arranged.

A specific aspect according to the present utility model, sample processing apparatus further include that opposite substrate can be slidably arranged Sliding seat, heating coil and/or magnet are arranged on sliding seat.Further, the glide direction of sliding seat can be upper and lower Direction.

According to the utility model, the setting of drive module is not particularly limited, it is preferred to use pneumatic actuation mode.Some In specific embodiment, drive module includes valve, air pump, pressure sensor and the flue being tightly connected with the opening of each chamber Air pump, pressure sensor, valve, extraction chamber are connected to by road, gas piping, and pressure sensor is connect with control module signal.

The utility model also provides a kind of digital pcr system comprising and mobile mechanism on the base is arranged in pedestal, if Operating platform on the base is set, the drop formation device with sampling probe, nucleic acid amplification temperature regulating device, product signal acquisition dress Set and control device, mobile mechanism, drop formation device, nucleic acid amplification temperature regulating device, product signal acquisition device respectively with Control device is connected and is controlled by control device, and digital pcr system further includes the utility model sample processing apparatus above-mentioned, The sample process consumptive material of middle sample processing apparatus is set on operating platform.

Preferably, the drive module of sample processing apparatus is connect with mobile mechanism, is moved under the drive of mobile mechanism.

In some specific and preferred embodiments, multiple extraction chambers in each processing unit are along digital pcr system Length direction be arranged side by side, the drive module of drop formation device and sample processing apparatus along digital pcr system width Direction arrangement.

In some specific and preferred embodiments, mobile mechanism has the fixed block being uprightly arranged, drive module packet The attachment base for being configured to be arranged on fixed block can be slided up and down to by including, and digital pcr system further includes for being drivingly connected seat The first longitudinal direction mobile device slided up and down.

In one embodiment, drive module includes valve, the gas being tightly connected with the opening of each chamber of sample process consumptive material Pump, pressure sensor and gas piping, valve, air pump, pressure sensor etc. are mounted on attachment base.

According to the utility model, when describing a component transverse to another component, it is intended that two components are to intersect, Typical situation includes that two components are perpendicular.

Due to the application of the above technical scheme, the utility model has the advantage that compared with prior art

The sample processing apparatus of the utility model can have the multiple functions such as mixing nucleic acid extraction and reagent, be applicable in Be directly used in the sample of digital pcr detection in obtaining, and the apparatus structure is simple, compact, it is small in size, can modularized design, Suitable for constructing unitary drop formula digital pcr system.

The digital pcr system collection sample process of the utility model droplet formation, expands, is detected on one, realizes from sample The automation control that drop formation arrives result detection to PCR reaction again is handled, reasonable in design, system overall volume subtracts It is small, meanwhile, reduce user's operation complexity, improve work efficiency, shorten detection cycle, reduces operating error.

Detailed description of the invention

Fig. 1 is that (pump and sensor in attached drawing only simply show for the stereoscopic schematic diagram of sample processing apparatus of embodiment 1 Meaning)；

Fig. 2 is the schematic top plan view of the sample processing apparatus of embodiment 1；

Fig. 3 is the schematic cross-sectional view in attached drawing 2 at A-A；

Fig. 4 is the stereoscopic schematic diagram of the reagent consumptive material of embodiment 1；

Fig. 5 is the stereoscopic schematic diagram of the sample processing apparatus of embodiment 2；

Fig. 6 is the stereoscopic schematic diagram of the valve base seat of embodiment 2；

Fig. 7 is the stereoscopic schematic diagram of the digital pcr system of embodiment 3；

Wherein: 1, pedestal；2, operating platform；21, drop receptacle mounting portion；100, drive module；110, air pump；120, 121,122,123,124,125,126,127,128,182, valve；130, gas piping；131, intake interface；140, pressure sensing Device；150, sealing element；160, pneumatic valve group；170, attachment base；180, valve base seat；181, sample process consumptive material interface；190, total Gas source interface；200, sample process consumptive material；210, substrate；211, first base material portion；212, the second base part；230, reagent consumes Material；231, shell；232, Reagent Tube；233, micro-pipe；234, plug；250, sample chamber；251, tabulator bars；260,261,262, 263,264,265,266,267,268, chamber is extracted；270, elongated interconnecting piece；271, first passage；272, second channel；280, Microchannel；281, heat conducting element；282, magnetic conductive component；3, drop formation mechanism；310, drop receptacle；700, first longitudinal direction is mobile Device；710, second longitudinal direction mobile device；8, product signal collecting mechanism；91, X direction guiding rail；92, mobile pedestal；93, Y-direction is led Rail；94, sliding seat；95, fixed block；951, the first sliding rail；952, the second sliding rail.

Specific embodiment

The novel sample processing apparatus that the utility model provides particularly suitable for digital pcr sample process, and organically combine Other modules of the novel sample processing apparatus and digital pcr system construct small in size, easy to operate, setting simple one Body formula digital pcr system.

In some embodiments, novel sample processing apparatus mainly includes sample process consumptive material and the sample Drive module, heating module and the control module of consumptive material connection are handled, drive module is for driving liquid at the sample It manages in each chamber or channel of consumptive material and flows, drive module connect with control module and controlled by control module.Drive module, The design of heating module and control module itself can be in the case where not needing any creative work by those skilled in the art Member is arranged according to the introduction of this paper and the knowledge known in this field grasped.

The technical solution of the utility model is further elaborated with specific embodiment with reference to the accompanying drawing, so that The advantages of the utility model, structure feature and working principle are easier to be readily appreciated by one skilled in the art, thus practical new to this The protection scope of type, which is made, apparent explicitly to be defined.

Embodiment 1

As shown in Figure 1 to Figure 3, a kind of sample processing apparatus comprising sample process consumptive material 200 and sample process consumptive material The drive module 100 and control module (not shown) of 200 connections, drive module 100 are for driving liquid to flow Device, drive module 100 connect with control module and are controlled by control module.

Sample process consumptive material 200 includes substrate 210 and reagent consumptive material 230, and the two is detachably connected.Further, sample Present treatment consumptive material 200 include first base material portion 211, the second base part 212, both ends respectively with first base material portion 211 and the second base The elongated interconnecting piece 270 that material portion 212 connects.Setting offers multiple intervals point along its length in first base material portion 211 Multiple extraction chambers 260 of cloth, are formed with sample chamber 250 in the second base part 212.Lead between multiple bottoms for extracting chamber 260 Cross the connection of microchannel 280 for being provided with 211 bottom of first base material portion.Near sample chamber 250 in multiple extraction chambers 260 Chamber 261 is extracted to be connected to sample chamber 250 by transfering channel.Transfering channel is included in first passage 271 and internal diameter less than first The second channel 272 in channel 271, first passage 271 are set to elongated interconnecting piece with extending along the length direction of elongated interconnecting piece 270 In 270, second channel 272 is formed in the second base part 212, and portion is connected to first passage 271 at one end with second channel 272, First passage 271, second channel 272 the other end be respectively communicated with and extract chamber 261 and sample chamber 250.Take the transfering channel Structure is more advantageous to the transfer for accurately and conveniently controlling quantitative liquid.

Interface is provided in the second base part 212, reagent consumptive material 230 includes shell 231, the U being arranged in shell 231 A pair of of the plug 234 for matching grafting on shell 231 with the interface of the second base part 212 is arranged in shape Reagent Tube 232.Reagent The inner cavity of pipe 232 constitutes reagent storage chamber, and the both ends of Reagent Tube 232 are connected separately with micro-pipe 233, and two micro-pipes 233 are worn respectively It crosses a pair of of plug 234 to be arranged, when plug 234 is correspondingly connected with interface, one inner cavity in two micro-pipes 233 is constituted will examination The microchannel that the inner cavity of agent pipe 232 and the bottom of sample chamber 250 are connected, another is for connecting drive module 100 or atmosphere.

In this example, the setting of the opening of chamber 260, sample chamber 250 and reagent storage chamber upward is extracted.One wherein It extracts and is provided with heat conducting element 281 at chamber 260, be provided with magnetic conductive component 282 at another adjacent extraction chamber 260.Thermally conductive member Part 281, magnetic conductive component 282 are elongated shape, they transverse to the corresponding axial line for extracting chamber 260 and have exposed respectively Contact portion.Heat conducting element 281 can be contacted with external heating coil, improve heating effect and rate, magnetic conductive component 282 can with it is outer Portion's magnet contact conducts magnetic force.Meanwhile heat conducting element 281, magnetic conductive component 282 have the intracorporal liquid of chamber at respective place Flow-disturbing effect, to be conducive to mix liquid.In addition, in sample chamber 250 be provided be at least partially situated in sample chamber 250 and The tabulator bars 251 being arranged transverse to the axial line of sample chamber 250, to improve mixed effect.

In this example, microchannel 280, the aperture of transfering channel are smaller, and microchannel, the aperture respectively about 90 of transfering channel are micro- Rice ~ 100 microns.When without outer power drive, liquid will not be transferred to another cavity from a cavity through channel, need by outer The driving of portion's drive module.As shown in Figure 1, Figure 3, the end of extracting section chamber 260 and micro-pipe 233 is connected to drive module respectively 100.Each drive module 100 respectively includes air pump 110, pressure sensor 140, valve 120 and gas piping 130.Extract chamber 260 Opening be provided with openable sealing element 150, valve 120 is tightly connected by sealing element 150 and sample process consumptive material 200. Although non-display control module in figure, those skilled in the art can be it will be readily apparent that the drive module 100 and control module After connection, then the opening and closing of each valve 120 can be controlled according to preset program by control module, make to generate just in required cavity Pressure or negative pressure, so that the intracorporal liquid of chamber is made to be transferred to another cavity from a cavity, it originally should be by people to be automatically performed Each processing operation that work is completed.

The sample processing apparatus of this example is very suitable for the processing of digital pcr sample, and an illustrative processing step is such as Under:

(1) it referring to Fig. 3, assists encapsulating dehydrated alcohol, extraction chamber 266 in oily, extraction chamber 267 extracting to encapsulate in chamber 268 Encapsulation cleaning solution 1 extracts chamber 265 and encapsulates cleaning solution 2, extracts chamber 264 and encapsulate cell pyrolysis liquid and enzyme, extract and be added in chamber 263 Measuring samples extract chamber 262 and encapsulate magnetic bead, extract chamber 261 and encapsulate eluent, encapsulate in the inner cavity of Reagent Tube 232 except nucleic acid is molten Different PCR reaction reagents such as polymerase, dNTP, PCR reaction reagent other than liquid is separated between mark substance with isolation oil, Both isolation oil, auxiliary oil are identical or different, not miscible with other substances, the specific oily phase group that may be selected with digital pcr drop At the same or similar formula oil；

(2) nucleic acid cleavage: valve 124 is opened, and is extracted chamber 264 and is connected with atmosphere, makes to extract the interior negative pressure that generates of chamber 263 for cell Chamber 263 is extracted in lysate and enzyme sucking, mixes, and heating is cracked；

(3) nucleic acid combines: after cracking, valve 123 is opened, and is made to extract generation negative pressure in chamber 262 and is inhaled cell pyrolysis liquid Enter to extract chamber 262 in conjunction with magnetic bead therein, and contact magnet with magnetic conductive component 261, realizes to mix and be adsorbed with magnetic bead, absorption Waste liquid afterwards pushes back extraction chamber 263 by microchannel 280 and is stored；

(4) nucleic acid cleans: corresponding valve 125, valve 126, the opening of valve 127 are connected with atmosphere when cleaning, make to extract in chamber 262 Generating negative pressure will be mixed, adsorbed and cleaned in corresponding cleaning solution sucking extraction chamber 262, and the waste liquid after cleaning pushes back former examination The storage of agent chamber；

(5) Nucleic Acid Elution: the opening of valve 121 is connected with atmosphere, makes to extract generation negative pressure in chamber 262 and extracts eluent sucking 262 chamber of chamber carries out Nucleic Acid Elution, after elution, pushes back the interior generation positive pressure of extraction chamber 262 by nucleic acid solution and extracts chamber 261；

(6) nucleic acid quantification shifts: the opening of valve 121 is connected with atmosphere, applies positive pressure to the auxiliary oil extracted in chamber 268, makes Auxiliary oil, which enters, to be extracted in chamber 261, so that the nucleic acid solution liquid level in it is risen to the port of transfering channel 270 or more, then valve 121 close and continue to fill nucleic acid in transfering channel 270, by pressure sensor the auxiliary oil pressurization extracted in chamber 268 Feedback coherent signal judges that filling finishes, and then valve 121 is opened, and making to extract generation negative pressure in chamber 268 drops nucleic acid solution liquid level It down to the port of transfering channel 270 hereinafter, then valve 121 is closed, extracts and generates positive pressure in chamber 268, will be turned using intracavity gas In the nucleic acid solution indentation sample chamber 250 in Mobile Communication road 270；

(7) it mixes: applying positive pressure in the PCR reagent indentation sample chamber 250 in Reagent Tube 232, carried out with nucleic acid solution In addition mixing can inhale with sampling probe or other liquid being directed in sample cavity 250 and be beaten, realize and mix, after mixing, apply Add positive pressure that the solution in sample chamber 250 is raised to the top of tabulator bars 251, solution divides upper layer and lower layer at this time, and upper layer is PCR sample This, lower layer is isolation oil；

(8) PCR sample pipettes: PCR sample that sampling probe is passed down through upper layer is inserted into draw solution in auxiliary oil, directly The continuation drawing section point auxiliary oil to after all siphoning away PCR sample or all siphon away.

The process of above-mentioned steps (2) to (8) can be automatically controlled all by control module and be completed, without human intervention.

Compared with existing sample processing apparatus especially digital pcr sample processing apparatus, the knot of the sample processing apparatus Structure is simple, can modularized design, small in size, setting is convenient, not only avoids that manual operation, to reduce work time-consuming, also reduces Operating error improves the accuracy of result.

Embodiment 2

The present embodiment provides a kind of sample processing apparatus, substantially with embodiment 1, in the present embodiment: for driving sample Have in the drive module and the specific design layout of the drive module of embodiment 1 that liquid flows in each cavity of present treatment consumptive material 200 Institute is different.As shown in figure 5, which show four groups of drive modules.Each drive module respectively includes air pump and (is not shown in the figure, gas Pump interface is connect with the intake interface 131 in figure by gas piping), gas sensor (be installed on inside attachment base 170, scheme In be not shown), pneumatic valve group 160 and gas piping (not shown).Wherein pneumatic valve group 160 includes solid with attachment base 170 Surely the valve base seat 180 connected, multiple valve 182(on valve base seat 180 and extraction chamber and sample chamber correspond).Valve base seat 180 inside are equipped with the channel for being connected to each 182 oral area of valve, and the lower position that the bottom of valve base seat 180 corresponds to each valve 182 is provided with Sample process consumptive material interface 181, these interfaces 181 are tightly connected with multiple extraction chambers and sample chamber by sealing element 150 respectively. Entire pneumatic valve group 160 is provided with a total gas source interface 190, which passes through gas piping and pressure sensing Device, air pump connection.Compared with Example 1, structure is more compact for the embodiment, and setting is more convenient, is more suitable for constructing integrated number Word PCR system.

Embodiment 3

The present embodiment provides a kind of digital pcr systems to be arranged on pedestal 1 as shown in fig. 7, the system includes pedestal 1 Operating platform 2 on the base is arranged in mobile mechanism, the drop formation device 3 with sampling probe 300, nucleic acid amplification temperature control dress It sets, product signal acquisition device 8, control device (not shown) and sample processing apparatus as described in Example 2.It is mobile Mechanism, sample processing apparatus, drop formation device 3, nucleic acid amplification temperature regulating device, product signal acquisition device 8 respectively with control Device is connected and is controlled by control device.

Specifically, mobile mechanism includes the X direction guiding rail 91 extended along the length direction of micro- digital pcr system, with X direction guiding rail The Y-direction guide rail extended on mobile pedestal 92 and along the width direction of digital pcr system is arranged in the mobile pedestal 92 being slidably connected 93, the sliding seat 94 being slidably connected with Y-direction guide rail 93.Sliding seat 94 further comprises the fixed block 95 being uprightly arranged, fixed block The first sliding rail 951, the second sliding rail 952 extended along up and down direction is respectively arranged on 95.The driving mould of sample processing apparatus The attachment base 170 and drop formation device 3 of block 100 are slidably connected with the first sliding rail 951, the second sliding rail 952 respectively.Digital pcr System further includes the first longitudinal direction mobile device 700 slided up and down for being drivingly connected seat 170, for driving drop formation device The 3 second longitudinal direction mobile devices 710 slided up and down, first longitudinal direction mobile device 700, second longitudinal direction mobile device 710 are without spy It does not limit, can be realized using motor driven leadscrew-nut mechanism or motor driven rack pinion structure.First longitudinal direction in this example Mobile device 700, second longitudinal direction mobile device 710 use motor driven leadscrew-nut mechanism, and the specific setting of the mechanism is normal Rule, herein without repeating.So set, the drive module 100 and drop formation device 3 of sample processing apparatus can carry out Front and rear, left and right, upper and lower three dimensions direction movement.Product signal acquisition device 8 is arranged on sliding seat 94, can move The movement in front and rear, left and right direction is carried out under the drive of motivation structure.

As shown in fig. 7, sample processing apparatus includes multiple sample process consumptive materials 200, the length of each sample process consumptive material 200 Direction is consistent with the length direction of digital pcr system.The drive module of drop formation device 3 and sample processing apparatus is then along number The width direction of word PCR system is arranged.This kind of layout structure is very compact, easy to operate.

In this example, drop formation device generates the generating mode of drop using microchannel vibration, and concrete structure design does not have It is restricted, set-up mode known in the art can be used.Operating platform 2 is equipped with drop receptacle mounting portion 21, is held by drop Device mounting portion 21 is removably installed drop receptacle 310, is used for following amplification reaction for obtaining with the cooperation of drop formation device 3 Drop and provide nucleic acid amplification reaction place.Product signal acquisition device 8 is known, including camera, optical fiber, exciting light This field conventional arrangement mode specifically can be used in turbin generator etc..

Include: using the operating procedure that the digital pcr system of the present embodiment is detected

Processing method with the PCR sample in embodiment 1 obtains sample and sampling；After sampling, drop formation mechanism 3 is driven To the position of drop receptacle, sampling probe 300 is inserted into drop receptacle 310 below oil phase liquid face, starts to be vibrated and pushed away sample, Make the drop that uniform size is generated in drop receptacle 310；After drop formation, nucleic acid amplification temperature regulating device is begun to warm up, and is carried out Nucleic acid amplification, after the completion of heat cycles, product signal acquisition device 8 is moved to drop receptacle position, is observed, is shot Photo is sent to control module and carries out data processing and analysis.

The above embodiments are only for explaining the technical ideas and features of the present invention, and its object is to allow be familiar with technique Personage can understand the content of the utility model and be implemented, do not limit the protection scope of the present invention, All equivalent change or modifications according to made by the spirit of the present invention essence, should all cover in the protection scope of the utility model It is interior.

Claims (24)

1. a kind of sample process consumptive material, it is characterised in that: the sample process consumptive material includes substrate, is arranged on the substrate One or more processing units, each processing unit include multiple extraction chambers, setting sample chamber on the substrate, setting Transfering channel between the sample chamber and the extraction chamber for the two to be connected to, the consumption of the reagent with reagent storage chamber Material, multiple extraction chambers are connected in bottom by microchannel, and the reagent storage chamber passes through micro- with the sample chamber Channel connection.

2. sample process consumptive material according to claim 1, it is characterised in that: the microchannel, transfering channel aperture point It Wei not be 60 microns ~ 100 microns.

3. sample process consumptive material according to claim 1, it is characterised in that: extraction chamber, sample chamber and the reagent The setting of the opening of storage chamber respectively upward.

4. sample process consumptive material according to claim 1 or 3, it is characterised in that: the sample process consumptive material further includes Set on the valve for extracting chamber opening, the valve is to close the extraction chamber or make the extraction chamber and atmosphere or extraction chamber External component conducting in addition.

5. sample process consumptive material according to claim 1, it is characterised in that: in each processing unit, there is one or more Heat conducting element and/or magnetic conductive component are provided on the corresponding substrate of a extraction chamber.

6. sample process consumptive material according to claim 1, it is characterised in that: the sample process consumptive material further includes at least It is positioned partially in the sample chamber and transverse to the tabulator bars of the axial line of sample chamber setting.

7. sample process consumptive material according to claim 1, it is characterised in that: the multiple extraction chamber is spaced apart, described Extract chamber, the axial line of sample chamber extends respectively along the short transverse of the substrate, the axial line transverse direction of the transfering channel In the axial line for extracting chamber, sample chamber.

8. sample process consumptive material according to claim 7, it is characterised in that: multiple extraction chambers edge of each processing unit The length direction of the substrate set gradually, the both ends of the transfering channel are respectively with the sample chamber, near institute State the extraction chamber connection of sample chamber.

9. sample process consumptive material according to claim 1 or claim 7, it is characterised in that: the transfering channel is included in one end The first passage and second channel being connected, wherein the internal diameter of first passage is greater than second channel, the other end of first passage It is connected to the extraction chamber, the other end of second channel is connected to the sample chamber.

10. sample process consumptive material according to claim 1 or claim 7, it is characterised in that: the substrate is described more including being formed with A extraction first base material portion of chamber, the second base part for being formed with the sample chamber, both ends respectively with the first base material portion and Second base part connection elongated interconnecting piece, the transfering channel include along elongated interconnecting piece length direction extend be set to institute State the first passage in elongated interconnecting piece.

11. sample process consumptive material according to claim 1, it is characterised in that: the reagent consumptive material and the substrate phase It is detachably connected.

12. sample process consumptive material according to claim 11, it is characterised in that: interface is provided on the substrate, it is described Reagent consumptive material include shell, the shell intracorporal U-shaped Reagent Tube is set, setting connects with the substrate on the housing Mouth matches a pair of of plug of grafting, and the inner cavity of the Reagent Tube constitutes the reagent storage chamber, and the two of the Reagent Tube End is connected separately with micro-pipe, and two micro-pipes are each passed through the pair of plug setting, when the reagent consumptive material and the base When material connects, one inner cavity in two micro-pipes is constituted the bottom of the inner cavity of the Reagent Tube and the sample chamber The microchannel of connection, another is the dress that liquid can be driven to flow for connecting drive module or atmosphere, the drive module It sets.

13. sample process consumptive material according to claim 1, it is characterised in that: the sample process consumptive material further includes one Kind or a variety of sample process needed for reagent, the reagent is packaged in selected from the multiple extraction chamber, the reagent storage chamber In cavity.

14. sample process consumptive material according to claim 13, it is characterised in that: the sample process consumptive material is PCR inspection Test sample present treatment consumptive material, each processing unit include 6 or more extraction chambers, and the sample process consumptive material further includes Set on the valve for extracting chamber opening, the valve is to close the extraction chamber or make the extraction chamber and atmosphere or extraction chamber External component in addition is connected, and in each processing unit, is provided with and leads on the corresponding substrate of the one or more extraction chamber The magnetic conductive component of thermal element and selectivity.

15. sample process consumptive material according to claim 14, it is characterised in that: one in multiple extraction chambers mentions It takes and is provided with heat conducting element on the corresponding substrate of chamber, be provided with magnetic conductive component on another adjacent corresponding substrate of extraction chamber, The heat conducting element, magnetic conductive component transverse to the axial line setting for extracting chamber and are respectively provided with exposed contact portion.

16. sample process consumptive material according to claim 15, it is characterised in that: the sample process consumptive material further includes being used for Substance needed for extracting nucleic acid, substance needed for the extraction nucleic acid includes cleaning solution, cell pyrolysis liquid, enzyme, eluent, magnetic Pearl, wherein cleaning solution, eluent, magnetic bead are packaged in respectively in different extraction chambers, and cell pyrolysis liquid is packaged in identical with enzyme It extracts in chamber or is packaged in different extraction chambers.

17. a kind of sample processing apparatus, it is characterised in that: including one or more such as any one of claim 1 to 16 institute The sample process consumptive material stated, the drive module and control module that connect with the sample process consumptive material, the drive module are used It is flowed in each chamber of the sample process consumptive material or channel in driving liquid, the drive module and the control module It connects and is controlled by the control module.

18. sample processing apparatus according to claim 17, it is characterised in that: the sample processing apparatus further includes heating Module, the heating module include heating coil, and the heating module connect with the control module and by the control mould Block control；And/or the sample processing apparatus further includes the magnet outside the extraction chamber.

19. sample processing apparatus according to claim 18, it is characterised in that: the sample processing apparatus further includes phase To the sliding seat that the substrate can be slidably arranged, the cunning is arranged in the heating coil and/or the magnet On dynamic seat.

20. sample processing apparatus according to claim 17, it is characterised in that: the drive module include with it is described each Valve, air pump, pressure sensor and the gas piping that the opening of chamber is tightly connected, the gas piping is by the air pump, pressure Sensor, valve, extraction chamber connection, the pressure sensor are connect with the control module signal.

21. a kind of digital pcr system, including pedestal, are arranged in the mobile mechanism on the pedestal, it is arranged on the pedestal Operating platform, the drop formation device with sampling probe, nucleic acid amplification temperature regulating device, product signal acquisition device and control dress Set, the mobile mechanism, drop formation device, nucleic acid amplification temperature regulating device, product signal acquisition device respectively with control device It connects and is controlled by the control device, it is characterised in that: the digital pcr system further includes as appointed in claim 17 to 20 The sample process consumptive material of sample processing apparatus described in one claim, the sample processing apparatus is set to the operation On platform.

22. digital pcr system according to claim 21, it is characterised in that: the driving mould of the sample processing apparatus Block is connect with the mobile mechanism, is moved under the drive of the mobile mechanism.

23. digital pcr system according to claim 22, it is characterised in that: multiple extractions in each processing unit Chamber is arranged side by side along the length direction of the digital pcr system, the drive module of drop formation device and sample processing apparatus It arranges along the width direction of the digital pcr system.

24. digital pcr system according to claim 22, it is characterised in that: the mobile mechanism has upright setting Fixed block, the drive module includes that can slide up and down to the attachment base being arranged on the fixed block, the number PCR system further includes the first longitudinal direction mobile device for driving the attachment base to slide up and down.