CN113341129B - Fluorescence immunoassay device and control method - Google Patents
Fluorescence immunoassay device and control method Download PDFInfo
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- CN113341129B CN113341129B CN202110647616.8A CN202110647616A CN113341129B CN 113341129 B CN113341129 B CN 113341129B CN 202110647616 A CN202110647616 A CN 202110647616A CN 113341129 B CN113341129 B CN 113341129B
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- 238000000034 method Methods 0.000 title claims description 13
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- 238000001514 detection method Methods 0.000 claims abstract description 39
- 239000003153 chemical reaction reagent Substances 0.000 claims description 109
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/533—Production of labelled immunochemicals with fluorescent label
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/04—Details of the conveyor system
Abstract
The invention discloses a fluorescence immunoassay device, which aims to solve the problems that in the prior art, a fluorescence immunoassay device is complex in structure and high in failure rate, so that the detection speed is low, and the actual high-precision and high-efficiency detection requirement cannot be met; the incubation module comprises a connecting rotating shaft vertically arranged on the base, the side wall of the connecting rotating shaft is rotationally connected with a conveying belt assembly and used for driving the connecting rotating shaft to horizontally rotate, the upper end and the lower end of the connecting rotating shaft are respectively connected with an incubation assembly and a lifting assembly, and the lifting assembly drives the connecting rotating shaft to move up and down; a first pushing module and a second pushing module are arranged around the incubation assembly, the first pushing module is used for continuously pushing the immunoreagent into the incubation assembly, and the second pushing module is used for continuously pushing and detecting the immunoreagent out of the incubation assembly.
Description
Technical Field
The invention belongs to the technical field of medical instruments, and particularly relates to a fluorescence immunoassay device and a control method.
Background
In the detection work of various proteins (antibodies, enzymes), hormones, medicines and microorganisms, the fluorescent immunoassay technology is often used for analysis and detection, and the fluorescent immunoassay technology has the advantages of strong specificity, high sensitivity, simple operation, low cost and the like, provides a real and reliable data basis for clinical disease diagnosis, prevention and treatment, and is used for inspection departments, inspection centers and the like of hospitals at all levels.
In the semiautomatic fluorescence immunoassay analyzer in the prior art, although the cost is lower, in the detection process, reagent strips are required to be manually put into the analyzer one by one for detection, and only one sample is loaded at a time, and the next sample can be loaded after the detection is finished, so that the automation degree is low and the detection efficiency is low; meanwhile, in the manual operation process, errors are easy to cause, and the detection efficiency and the detection precision are affected.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention aims to provide a fluorescence immunoassay device and a control method, and aims to solve the problems that the fluorescence immunoassay device in the prior art is complex in structure, high in failure rate, low in detection speed and incapable of meeting the practical high-precision high-efficiency detection requirement.
The invention is realized by adopting the following technical scheme:
a fluorescence immunoassay device comprising: the device comprises a base, wherein an incubation module for incubating immune reagents at constant temperature, at least one pushing module and at least one pushing module are arranged on the base;
the incubation module comprises a connecting rotating shaft vertically arranged on the base, the side wall of the connecting rotating shaft is rotationally connected with a conveying belt assembly and used for driving the connecting rotating shaft to horizontally rotate, the upper end and the lower end of the connecting rotating shaft are respectively connected with an incubation assembly and a lifting assembly, and the lifting assembly drives the connecting rotating shaft to move up and down; a first pushing module and a second pushing module are arranged around the incubation assembly, the first pushing module is used for continuously pushing the immunoreagent into the incubation assembly, and the second pushing module is used for continuously pushing and detecting the immunoreagent out of the incubation assembly.
In order to optimize the technical scheme, the specific measures adopted further comprise:
further, the lifting assembly comprises a connecting plate and a screw rod stepping motor, one end of the connecting plate is connected with a bearing between the connecting rotating shafts, and the other end of the connecting plate is rotationally connected with the screw rod stepping motor to drive the connecting plate to move up and down.
Further, the incubation subassembly includes that the multilayer is hatched the dish and is covered and establish the heat preservation cover on the dish is hatched to the multilayer, it is connected pivot fixed connection to incubate the dish with, it is used for inserting the cultivation groove of cultivating immunological reagents to incubate to set up on the dish, demountable installation has the setting element on the cultivation groove, heat preservation cover lateral wall is provided with a plurality of heating plates.
Further, the first pushing module comprises a sliding component, the sliding component comprises a first sliding rail fixedly installed on a base, a conveying sliding block is connected to the sliding rail in a sliding mode, a first synchronous belt component which is horizontally driven is arranged on one side of the sliding rail and connected with the conveying sliding block, a pushing component for pushing immune reagents is arranged on the conveying sliding block, a first base plate is arranged above the base, a sliding groove is formed in the first base plate, an end cover is arranged above the sliding groove, and a first sliding way for the immune reagents to move is formed in the end cover.
Further, the pushing component comprises a fixed table, the fixed table is fixedly arranged on the conveying sliding block, a clamping hook for pushing the immunoreagent strip is rotationally connected to the fixed table, the clamping hook is in a vertical direction in a free state, and the clamping hook can rotate in a unidirectional ninety degrees in a vertical plane.
Further, the device also comprises a limiting assembly, wherein the limiting assembly comprises a baffle plate, the baffle plate is fixedly arranged on the side wall of the second substrate, a vertically arranged limiting door is arranged between the baffle plate and the second substrate, an inlet is formed in the limiting door, and a pressing plate is arranged at the lower end of the limiting door; the conveying slide block is provided with a boss, the pressing plate is in contact with the boss, and the pressing plate is pushed up and down through the forward and backward movement of the boss, so that the first slideway is opened and closed.
Further, the second pushing module comprises a second sliding rail fixedly mounted on the base, a card withdrawing sliding block is connected to the second sliding rail in a sliding manner, a second synchronous belt assembly is mounted on one side of the second sliding rail, the second synchronous belt assembly is fixedly connected with the card withdrawing sliding block, an objective table which is horizontally arranged and used for placing fluorescent reagents is mounted on the card withdrawing sliding block, a clamping hook assembly for fixing the fluorescent reagents is mounted on the objective table, a second substrate fixedly connected with the base is arranged above the clamping hook assembly, a second sliding way for moving the fluorescent reagents is formed in the second substrate, a positioning piece for fixing the fluorescent reagents is mounted in the second sliding way, and an optical detection assembly for detecting the fluorescent reagents is mounted on the second substrate.
Further, the hook component comprises a rotating shaft, triangular protrusions are arranged on the rotating shaft, torsion springs are arranged between the protrusions and the rotating shaft, the rotating shaft is inserted into two sides of the objective table, grooves are formed in two ends of the objective table, and the protrusions can retract into the grooves under the driving of the rotating shaft.
Further, the optical detection assembly comprises a shell, a first through hole in the vertical direction is formed in the shell, a PD sensor, a first plano-convex lens, a filter, a spectroscope and a second plano-convex lens are sequentially arranged in the through hole from top to bottom, a second through hole in the horizontal direction is formed in one side of the spectroscope facing to the spectroscope, and a light source emitter is arranged in the second through hole.
A method for controlling a fluorescence immunoassay apparatus, comprising the steps of:
step S1: according to the length of the first sliding rail and the length of the fluorescent reagent, calculating the rotating speed of the first synchronous belt assembly and the frequency of placing the fluorescent reagent, so that the clamping hook assembly conveys one fluorescent reagent at a time;
step S2: calculating the rotating speed of the conveyor belt assembly according to the time of pushing the fluorescent reagent to the incubation groove of the incubation plate, so that when each fluorescent reagent enters the incubation plate, the next empty incubation groove of the incubation plate is aligned with the fluorescent reagent to be incubated;
step S3: the incubation plate carries out constant temperature and humidity incubation according to preset temperature and humidity;
step S4: and calculating the rotating speed of the second synchronous belt assembly according to the position of the optical detection assembly on the second substrate, the detection time, the length of the second substrate, the length of the fluorescent reagent and the rotating speed of the conveyor belt assembly, so that when the next fluorescent reagent moves to the detection position, the last fluorescent reagent completes detection, and the last fluorescent reagent is pushed out of the second pushing module through the next fluorescent reagent.
The invention has the beneficial effects that:
1. compared with the prior art, the fluorescence immunoassay device does not need to manually put reagent strips into the incubation device one by one and manually take out the incubated reagent strips, realizes the highly-automatic putting-in and taking-out process through reasonable structural layout design, pushes the reagent strips step by the structural layout of the pushing module and the pushing module, reduces the stroke distance of single pushing, improves the accuracy of pushing, reduces the failure rate of pushing, has a simple structure and small occupied area, greatly improves the working efficiency, and integrally realizes the highly-automatic process of automatic pushing, automatic incubation, automatic pushing and automatic detection of fluorescent reagents.
2. Compared with the prior art, the incubation module of the device can flexibly move in the three-dimensional space through the incubation plate with the rotatable lifting multilayer structure when the incubation position is increased, and can meet the requirement that the immunoreagent is quickly inserted in the state of not completely aligning with the notch in cooperation with the open slot design of the incubation plate, so that the incubation efficiency is further improved.
Drawings
FIG. 1 is a schematic diagram of the structure of an analysis device according to the present invention.
Fig. 2 is a schematic structural diagram of the first pushing module in fig. 1 according to the present invention.
Fig. 3 is a schematic view of a part of the structure of the first pushing module in fig. 2 according to the present invention.
FIG. 4 is a schematic diagram of the structure of the incubation module of FIG. 1 according to the invention.
Fig. 5 is a schematic front view of fig. 4 of the present invention.
Fig. 6 is a schematic structural diagram of the second pushing module in fig. 1 according to the present invention.
Fig. 7 is a schematic view of a portion of the structure of fig. 6 according to the present invention.
FIG. 8 is a cross-sectional view of the optical detection assembly of FIG. 6 in accordance with the present invention.
The reference numerals are: base 10, incubation module 20, incubation assembly 21, incubation plate 211, incubation slot 2111, positioning member 2112, thermal cover 212, mounting member 213, conveyor belt assembly 22, rotation motor 221, coupling 222, drive pulley 223, timing belt 224, driven pulley 225, flat key sleeve 226, mounting base 227, lifting assembly 23, connection plate 231, lead screw stepper motor 232, connection shaft 24, first push module 30, first main substrate 31, first slide rail 32, transport slide 33, first timing belt assembly 34, timing pulley 341, first shaft 342, first protrusion 343, coupling 344, drive motor 345, toothed timing belt 346, push module 35, fixing base 351, hook 352, first sub-substrate 36, end cap 37, limit assembly 38, baffle 381, limit door 382, pressure plate 383, boss 384, second push module 40, second main substrate 41, second slide rail 42, lead screw stepper motor 43, second timing belt assembly 44, stage 45, groove 451, hook assembly 46, shaft 461, protrusion 47, second sub-substrate detection member 49, toothed timing belt 346, second flat lens 497, light source 493, light source 497.
Detailed Description
Embodiments of the present invention are described in further detail below with reference to the accompanying drawings.
As shown in fig. 1, the present invention provides a fluorescence immunoassay device, which is a process for automatically pushing, incubating, pushing out and detecting fluorescent reagents, and the apparatus comprises a base 10, an incubation module 20 on which a constant temperature incubation fluorescent reagent 50 is disposed on the base 10, at least one first pushing module 30 and at least one second pushing module 40.
Referring to fig. 1-3 again, the incubation module 20 includes an incubation assembly 21, a conveyor belt assembly 22 for driving the incubation assembly 21 to horizontally rotate, a lifting assembly 23 for driving the incubation assembly 21 to move up and down, and a connection shaft 24 for transmitting power of the conveyor belt assembly 22 and the lifting assembly 23 to the incubation assembly 21.
The incubation component 21 comprises an incubation plate 211 with a multilayer structure, a plurality of incubation grooves 2111 for inserting fluorescent reagents 50 are formed in the incubation plate 211, the incubation grooves 2111 are formed along the outer ring of the incubation plate 211, the incubation grooves 2111 are distributed at equal intervals in the circumferential direction, and the size distribution of the intervals between the incubation grooves 2111 is continuously set according to actual requirements, so that specific requirements are not met in the embodiment. The incubation groove 2111 is provided with a gap at one side close to the center of the incubation plate 211, and is used for manually pushing out the fluorescent reagent 50 when a machine fails, a notch at one side of the incubation groove 2111 far away from the gap is designed to be in a horn-shaped opening shape and is used for expanding an interface for inserting the fluorescent reagent 50, when the fluorescent immunoassay device generates fine displacement due to vibration or shaking and other factors and is aligned with the notch of the incubation groove, the fluorescent reagent 50 can be smoothly pushed into the incubation groove 2111, a positioning piece 2112 is detachably arranged above the incubation groove 2111, and the positioning piece 2112 is used for fixing the movement in a vertical plane of the fluorescent reagent 50, namely the fluorescent reagent 50 is tightly pressed in the incubation groove 2111; the incubation plate 211 is externally provided with a thermal insulation cover 212, a plurality of heating plates (not shown) are arranged on the inner wall of the thermal insulation cover 212, and the air in the thermal insulation cover 212 is heated to a set temperature through the heating plates, so that the fluorescent reagent 50 is incubated at a constant temperature, the specific temperature setting can be adjusted according to incubation temperatures required by different fluorescent reagents 50, and the specific setting is not performed in the scheme.
A mounting piece 213 is arranged at the center of the incubation plate 211, the mounting piece 213 adopts a cylindrical hollow tubular structure, and the lower end of the mounting piece 213 is connected with the upper end of the connecting rotating shaft 24 through a bolt; when the incubation plate 211 needs to be replaced and overhauled, the disassembly is convenient.
The conveyer belt subassembly 22 sliding connection is in the middle of connecting pivot 24, conveyer belt subassembly 22 includes rotation motor 221, and this rotation motor 221 fixed mounting is on base 10, and rotation motor 221 output shaft is connected with driving pulley 223 through shaft coupling 222, and this driving pulley 223 is connected with driven pulley 225 through hold-in range 224, the size of driven pulley 225 is greater than the size of driving pulley 223, can increase the transmission ratio, improves transmission efficiency. The driven pulley 225 is connected with the connection rotating shaft 24 which is vertically arranged, the connection rotating shaft 24 is connected with the connection rotating shaft sleeve 241 in a sliding manner (namely, the connection rotating shaft 24 can slide up and down relative to the connection rotating shaft sleeve 241), the flat key sleeve 226 is arranged on the outer side of the connection rotating shaft sleeve 241, the connection rotating shaft sleeve 241 is connected with the flat key sleeve 226 through a flat key, the driven pulley 225 is fixedly connected with the flat key sleeve 226 through a bolt, so that the flat key sleeve 226 is driven to rotate horizontally by the driven pulley 225, bearings are arranged on the upper end and the lower end outside the flat key sleeve 226, an installation seat 227 for wrapping the whole flat key sleeve 226 is arranged on the outer side of the bearing, and the flat key sleeve 226 and the installation seat 227 can rotate relatively through the bearings. It can be understood that: the connection shaft 24 can be driven to horizontally rotate by the rotation motor 221.
The lower extreme of connecting pivot 24 is connected with lifting unit 23, lifting unit 23 includes connecting plate 231, connecting plate 231 is connected with the lower extreme bearing of connecting pivot 24, and this connecting plate 231 sets up with base 10 in parallel, is connected with the output shaft of lead screw step motor 232 at the opposite side of connecting plate 231, and this lead screw step motor 232 body fixed mounting is on base 10, can understand: the screw rod stepping motor 232 drives the connecting rotating shaft 24 to move up and down, so that the incubation assembly 21 at the upper end of the connecting rotating shaft 24 is driven to move to a required position.
At least two channels are formed on the heat insulation cover 212 of the incubation tray 211, the channels correspond to one incubation groove 2111 of the incubation tray 211, and the channels are connected to the first pushing module 30 and the second pushing module 40.
Referring to fig. 4-5, the first pushing module 30 includes a first main substrate 31 disposed above the base 10 and fixedly connected to the base 10, a first sliding rail 32 is mounted on the first main substrate 31, a conveying slider 33 is slidably connected to the first sliding rail 32, a first synchronous belt assembly 34 that horizontally rotates is disposed on one side of the first sliding rail 32, the first synchronous belt assembly 34 is fixedly connected to the conveying slider 33, a pushing assembly 35 that pushes the fluorescent reagent 50 is disposed on the conveying slider 33, the pushing assembly 35 includes a fixing table 351, the fixing table 351 is fixedly disposed on the conveying slider 33, a hook 352 that pushes the fluorescent reagent 50 is rotatably connected to the fixing table 351, and the hook 352 can rotate in a vertical plane in a direction opposite to a direction in which the fluorescent reagent 50 moves; the base 10 top is provided with first vice base plate 36, the spout that supplies the propelling movement subassembly to remove is offered to first vice base plate 36, the spout top is provided with end cover 37, offer the first slide that supplies fluorescent reagent 50 to remove in the end cover 37, the spout is connected with first slide and is link up. It can be understood that: the fluorescent reagent 50 is pushed to move unidirectionally by the hook 352, and simultaneously, the hook 352 is matched with the rotation of the hook 352 opposite to the movement direction of the fluorescent reagent 50, so that the hook 352 can continuously push the fluorescent reagent 50 into the culture groove 2111 of the incubation tray 211.
A limiting assembly 38 is further mounted on the side wall of the first substrate 35, the limiting assembly 38 comprises a baffle 381, the baffle 371 is fixedly mounted on the side wall of the first auxiliary substrate 36, a vertically arranged limiting door 382 is mounted between the baffle 381 and the first auxiliary substrate 36, an inlet is formed in the limiting door 382, and a pressing plate 383 is arranged at the lower end of the limiting door 382; the conveying slide block 33 is provided with a boss 384, the pressing plate 383 is in contact with the boss 384, and the pressing plate 383 is pushed up and down by the forward and backward movement of the boss 384, so as to realize the opening and closing of the first slide way. It can be understood that: by opening and closing the first slide way through the limit door 382, only one fluorescent reagent 50 is transported at a time, and the condition that two fluorescent reagents 50 are transported simultaneously and no incubation groove 2111 is stored is avoided.
Referring to fig. 6-8, the second pushing module 40 includes a second main substrate 41 fixedly mounted on the base 10, a second slide rail 42 is disposed on the second main substrate 41, a card withdrawing slider 43 is slidably connected on the second slide rail 42, a second synchronous belt assembly 44 is mounted on one side of the second slide rail 42, the second synchronous belt assembly 44 is fixedly connected with the card withdrawing slider 43, a horizontally disposed stage 45 for placing the fluorescent reagent 50 is mounted on the card withdrawing slider 43, a hook assembly 46 for fixing the fluorescent reagent 50 is mounted on the hook assembly 46, the hook assembly 46 includes a rotating shaft 461, a triangular protrusion 462 is mounted on the rotating shaft 461, a torsion spring (not shown) is disposed between the protrusion 462 and the rotating shaft 461, the rotating shaft 461 is inserted on two sides of the stage 45, grooves 451 are formed at two ends of the stage 45, and the protrusion 462 can be retracted into the grooves 451 of the stage 45 under the driving of the rotating shaft 461; a second auxiliary substrate 47 fixedly connected with the base 10 is arranged above the objective table 45, a second slideway for moving the fluorescent reagent 50 is arranged on the second substrate 47, a positioning piece 48 for fixing the fluorescent reagent 50 is arranged in the second slideway, and an optical detection assembly 49 for detecting the fluorescent reagent 50 is arranged on the second substrate 47.
The optical detection assembly 49 includes a housing 491, a first through hole in a vertical direction is formed in the housing 491, a PD sensor 492, a first plano-convex lens 493, a filter 494, a spectroscope 495 and a second plano-convex lens 496 are sequentially installed in the through hole from top to bottom, a second through hole in a horizontal direction is formed in a side of the mirror surface of the spectroscope 495 facing to the mirror surface, and a light source emitter 497 is installed in the second through hole; the filter 494 adopts a 610nm band-pass filter, the spectroscope 495 adopts a 365 band-pass spectroscope, and the angle of the spectroscope 495 is forty-five degrees; it can be understood that: the light source emitter 497 emits 365nm laser, then reflects and irradiates downwards through forty-five degrees of the spectroscope 495, then uses the second plano-convex lens 496 to concentrate the laser to the surface of the fluorescent reagent 50, then the fluorescent reagent 50 is stimulated to emit 610nm light, the light is converted into parallel light through the second plano-convex lens 496 in sequence, the parallel light is directly radiated through the spectroscope 495, other light except 610nm is filtered through the filter 494, and finally the light is focused on the PD sensor 492 through the first plano-convex lens 493. Wherein the beam splitter 495 passes the reflected beam directly due to the angular setting.
The first synchronous belt assembly 34 and the second synchronous belt assembly 44 have the same structure, taking the first synchronous belt assembly 34 as an example, the first synchronous belt assembly 34 comprises two synchronous pulleys 341, the size of the synchronous pulleys 341 is equal, the two synchronous pulleys 341 are arranged at two ends of the same side of the first sliding rail 32 in parallel, meanwhile, the synchronous pulleys 341 are vertically and fixedly arranged on the upper surface of the base 10, a first rotating shaft 342 is inserted between one of the synchronous pulleys 341, the first rotating shaft 342 is fixedly arranged on the base 10 through a first protruding block 343, a driving motor 345 is connected below one of the synchronous pulleys 341 through a coupling 344, a shell of the driving motor 345 is fixedly arranged on the lower surface of the base 10, and a toothed synchronous belt 346 is arranged between the synchronous pulleys 341. The toothed timing belt 346 is fixedly connected to the transfer slider 33. It can be understood that: the transfer slider 33 is moved forward and backward by the toothed timing belt 346, and the guiding function of the moving direction is achieved by the first slide rail 32.
The working principle of the invention is as follows:
firstly, the conveying sliding block 33 is moved to one end close to the limiting door 382, the limiting door 382 is pushed upwards to the inlet of the limiting door 382 through the boss 384 to be aligned with the first slideway, then the fluorescent reagent 50 is inserted into the end cover 37 from the inlet, the clamping hook 352 is in a horizontal state under the pressing of the fluorescent reagent 50, when the fluorescent reagent 50 is completely inserted into the end cover 37, the clamping hook 352 is in a vertical state and is positioned at the outer side of one end of the fluorescent reagent 50 close to the limiting door 382, then the driving motor 345 is controlled to drive the tooth-shaped synchronous belt 346 to rotate, the tooth-shaped synchronous belt 346 drives the conveying sliding block 33 to move towards the direction of the incubation tray 211, the clamping hook 352 pushes the fluorescent reagent 50 into the incubation slot 2111 in the incubation tray 211, meanwhile, the limiting door 382 moves downwards under the action of gravity due to the inclined surface structure of the boss 384, so that the first slideway is sealed, and the condition that only one fluorescent reagent 50 is conveyed at one time is ensured, and the condition that two fluorescent reagents 50 are conveyed simultaneously is avoided, and no incubation slot 2111 is stored is caused; then, the driving motor 345 rotates reversely to drive the conveying slide 33 to exit the incubation groove 2111 for pushing the next fluorescent reagent 50. The fluorescent reagent 50 is continuously pushed to the incubation plate 211, so that the degree of automation is high and the accuracy is high.
When the fluorescent reagent 50 enters the incubation groove 2111 in the incubation plate 211, the rotation motor 221 drives the incubation plate 211 to rotate horizontally once, so that the next empty incubation groove 2111 is aligned with the first pushing module so as to meet the next fluorescent reagent 50, and when the incubation groove 2111 of one layer of incubation plate 211 is filled, the screw rod stepping motor 232 drives the incubation plate 211 to move up and down, so that the incubation plate 211 with the empty incubation groove 2111 meets the new fluorescent reagent 50. The fluorescent reagent 50 is incubated at a constant temperature in the incubation groove 2111 according to a set temperature, and the incubated fluorescent reagent 50 is pushed out of the incubation plate 211 by the second pushing module 40 and detected.
The specific process of pushing out the detection by the second pushing module 40 is as follows: firstly, the second synchronous belt assembly 44 is started, then the second synchronous belt assembly 44 drives the card withdrawing slide block 43 and the objective table 45 to move towards the incubation tray 211, when the objective table 45 moves to the position below the fluorescent reagent 50 after incubation is completed, the objective table 45 is pressed into a groove 451 on the objective table 45 by the fluorescent reagent 50 due to the triangular structure of the projection 462, then the objective table 45 continues to move towards the incubation tray, when the objective table 45 moves to the right, the projection 462 is abutted against the side wall of the fluorescent reagent 50, namely, the projection 462 is in a vertical state, then the second synchronous belt assembly 44 is controlled to move reversely, the objective table 45 drives the fluorescent reagent 50 to move towards the direction away from the incubation tray 211, and when the fluorescent reagent 50 moves to the position corresponding to the optical detection assembly 49 inside the second auxiliary substrate 47, the objective table is fixed at the current position through the extrusion action of the positioning piece 48, and then the optical detection assembly 49 is utilized for detection; while the second timing belt assembly 44 again reverses direction to effect the snagging of the next fluorescent reagent 50. When the next fluorescent reagent 50 is pushed by the projection 462 to the detection position, the last fluorescent reagent 50 has been detected and is pushed out of the present immunoassay device completely by the next fluorescent reagent 50. The fluorescent reagent 50 after incubation is continuously pushed out from the incubation plate 211 and detected, the fluorescent reagent 50 is pushed out and the fluorescent reagent 50 is detected, and the fluorescent reagent 50 is combined by one structural device, so that the degree of automation is high, and the detection efficiency is greatly improved.
A control method of a fluorescence immunoassay analyzer, comprising the steps of:
step S1: calculating the rotation speed of the first synchronous belt assembly 33 and the frequency of placing the fluorescent reagent 50 according to the length of the first sliding rail 31 and the length of the fluorescent reagent 50, so that the clamping hook assembly can convey one fluorescent reagent at a time;
step S2: calculating the rotating speed of the conveyor belt assembly according to the time of pushing the fluorescent reagent to the incubation groove of the incubation plate, so that when each fluorescent reagent enters the incubation plate, the next empty incubation groove of the incubation plate is aligned with the fluorescent reagent to be incubated;
step S3: the incubation plate carries out constant temperature and humidity incubation according to preset temperature and humidity;
step S4: and calculating the rotating speed of the second synchronous belt assembly according to the position of the optical detection assembly on the second substrate, the detection time, the length of the second substrate, the length of the fluorescent reagent and the rotating speed of the conveyor belt assembly, so that when the next fluorescent reagent moves to the detection position, the last fluorescent reagent completes detection, and the last fluorescent reagent is pushed out of the second pushing module through the next fluorescent reagent.
The above is only a preferred embodiment of the present invention, and the protection scope of the present invention is not limited to the above examples, and all technical solutions belonging to the concept of the present invention belong to the protection scope of the present invention. It should be noted that modifications and adaptations to the invention without departing from the principles thereof are intended to be within the scope of the invention as set forth in the following claims.
Claims (5)
1. A fluorescence immunoassay device, comprising: the device comprises a base, wherein an incubation module for incubating immune reagents at constant temperature, at least one pushing module and at least one pushing module are arranged on the base;
the incubation module comprises a connecting rotating shaft vertically arranged on the base, the side wall of the connecting rotating shaft is rotationally connected with a conveying belt assembly and used for driving the connecting rotating shaft to horizontally rotate, the upper end and the lower end of the connecting rotating shaft are respectively connected with an incubation assembly and a lifting assembly, and the lifting assembly drives the connecting rotating shaft to move up and down; a first pushing module and a second pushing module are arranged around the incubation assembly, the first pushing module is used for continuously pushing the immunoreagent into the incubation assembly, and the second pushing module is used for continuously pushing and detecting the immunoreagent from the incubation assembly;
the first pushing module comprises a sliding component, the sliding component comprises a first sliding rail fixedly installed on a base, a conveying sliding block is connected to the sliding rail in a sliding manner, a first synchronous belt component which is horizontally driven is arranged on one side of the sliding rail, the first synchronous belt component is connected with the conveying sliding block, a pushing component for pushing immune reagent is arranged on the conveying sliding block, a first substrate is arranged above the base, a sliding groove is formed in the first substrate, an end cover is arranged above the sliding groove, and a first sliding way for the immune reagent to move is formed in the end cover;
the pushing assembly comprises a fixed table, the fixed table is fixedly arranged on the conveying sliding block, a clamping hook for pushing the immunoreagent strip is rotationally connected to the fixed table, the clamping hook is in a vertical direction in a free state, and the clamping hook can rotate in a unidirectional ninety degrees in a vertical plane;
the device comprises a first substrate, a second substrate, a limiting assembly, a pressing plate and a limiting assembly, wherein the limiting assembly comprises a baffle plate which is fixedly arranged on the side wall of the second substrate, a vertically arranged limiting door is arranged between the baffle plate and the second substrate, an inlet is formed in the limiting door, and the lower end of the limiting door is provided with the pressing plate; the conveying slide block is provided with a boss, the pressing plate is contacted with the boss, and the pressing plate is pushed up and down through the forward and backward movement of the boss, so that the first slideway is opened and closed;
the second pushing module comprises a second sliding rail fixedly arranged on the base, a card withdrawing sliding block is connected to the second sliding rail in a sliding way, a second synchronous belt assembly is arranged on one side of the second sliding rail, the second synchronous belt assembly is fixedly connected with the card withdrawing sliding block, an objective table which is horizontally arranged and used for placing fluorescent reagents is arranged on the card withdrawing sliding block, a clamping hook assembly for fixing the fluorescent reagents is arranged on the objective table, a second substrate fixedly connected with the base is arranged above the clamping hook assembly, a second sliding way for moving the fluorescent reagents is arranged on the second substrate, a positioning piece for fixing the fluorescent reagents is arranged in the second sliding way, and an optical detection assembly for detecting the fluorescent reagents is arranged on the second substrate;
the clamping hook assembly comprises a rotating shaft, triangular protrusions are arranged on the rotating shaft, torsion springs are arranged between the protrusions and the rotating shaft, the rotating shaft is inserted into two sides of the objective table, grooves are formed in two ends of the objective table, and the protrusions can retract into the grooves under the driving of the rotating shaft.
2. The fluorescence immunoassay device of claim 1, wherein: the lifting assembly comprises a connecting plate and a screw rod stepping motor, one end of the connecting plate is connected with a bearing between the connecting rotating shafts, and the other end of the connecting plate is rotationally connected with the screw rod stepping motor to drive the connecting plate to move up and down.
3. The fluorescence immunoassay device of claim 1, wherein: the incubation subassembly includes that the multilayer is hatched the dish and is covered and establish the heat preservation cover on the dish is hatched to the multilayer, it is connected with connecting the pivot fixed connection to incubate the dish, it is used for inserting the cultivation groove of cultivating immunological reagents to incubate to set up on the dish, demountable installation has the setting element on the cultivation groove, heat preservation cover lateral wall is provided with a plurality of heating plates.
4. The fluorescence immunoassay device of claim 1, wherein: the optical detection assembly comprises a shell, a first through hole in the vertical direction is formed in the shell, a PD sensor, a first plano-convex lens, a filter, a spectroscope and a second plano-convex lens are sequentially arranged in the through hole from top to bottom, a second through hole in the horizontal direction is formed in one side of the spectroscope facing to the spectroscope, and a light source emitter is arranged in the second through hole.
5. A control method of the fluorescence immunoassay apparatus according to claim 3, comprising the steps of:
step S1: according to the length of the first sliding rail and the length of the fluorescent reagent, calculating the rotating speed of the first synchronous belt assembly and the frequency of placing the fluorescent reagent, so that the clamping hook assembly conveys one fluorescent reagent at a time;
step S2: calculating the rotating speed of the conveyor belt assembly according to the time of pushing the fluorescent reagent to the incubation groove of the incubation plate, so that when each fluorescent reagent enters the incubation plate, the next empty incubation groove of the incubation plate is aligned with the fluorescent reagent to be incubated;
step S3: the incubation plate carries out constant temperature and humidity incubation according to preset temperature and humidity;
step S4: and calculating the rotating speed of the second synchronous belt assembly according to the position of the optical detection assembly on the second substrate, the detection time, the length of the second substrate, the length of the fluorescent reagent and the rotating speed of the conveyor belt assembly, so that when the next fluorescent reagent moves to the detection position, the last fluorescent reagent completes detection, and the last fluorescent reagent is pushed out of the second pushing module through the next fluorescent reagent.
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| CN115184592B (en) * | 2022-06-24 | 2023-09-29 | 普迈德（北京）科技有限公司 | Immunoassay detection equipment and detection method |
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