CN113341129A - Fluorescence immunoassay device and control method - Google Patents
Fluorescence immunoassay device and control method Download PDFInfo
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- CN113341129A CN113341129A CN202110647616.8A CN202110647616A CN113341129A CN 113341129 A CN113341129 A CN 113341129A CN 202110647616 A CN202110647616 A CN 202110647616A CN 113341129 A CN113341129 A CN 113341129A
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- 238000003018 immunoassay Methods 0.000 title claims abstract description 18
- 238000000034 method Methods 0.000 title claims description 13
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 118
- 238000011534 incubation Methods 0.000 claims abstract description 100
- 238000001514 detection method Methods 0.000 claims abstract description 41
- 230000001360 synchronised effect Effects 0.000 claims description 37
- 239000000758 substrate Substances 0.000 claims description 22
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- 238000004321 preservation Methods 0.000 claims description 7
- 230000001900 immune effect Effects 0.000 claims description 5
- 238000009434 installation Methods 0.000 claims description 5
- 238000010438 heat treatment Methods 0.000 claims description 4
- 230000036039 immunity Effects 0.000 claims description 4
- 238000007373 indentation Methods 0.000 claims description 2
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- 238000010586 diagram Methods 0.000 description 4
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- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
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- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
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- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/533—Production of labelled immunochemicals with fluorescent label
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
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Abstract
The invention discloses a fluorescence immunoassay device, which aims to solve the problems that in the prior art, a fluorescence immunoassay instrument has a complex structure and a high failure rate, so that the detection speed is not high, and the actual high-precision and high-efficiency detection requirements cannot be met; the incubation module comprises a connecting rotating shaft vertically arranged on the base, the side wall of the connecting rotating shaft is rotatably connected with a conveying belt assembly for driving the connecting rotating shaft to rotate horizontally, the upper end and the lower end of the connecting rotating shaft are respectively connected with an incubation assembly and a lifting assembly, and the lifting assembly drives the connecting rotating shaft to move up and down; and a first pushing module and a second pushing module are arranged around the incubation assembly, the first pushing module is used for continuously pushing the immune reagent into the incubation assembly, and the second pushing module is used for continuously pushing the immune reagent out of the incubation assembly and detecting the immune reagent.
Description
Technical Field
The invention belongs to the technical field of medical instruments, and particularly relates to a fluorescence immunoassay device and a control method.
Background
In the detection work of various proteins (antibodies, enzymes), hormones, drugs and microorganisms, the fluorescence immunoassay technology is often used for analysis and detection, has the advantages of strong specificity, high sensitivity, simple and convenient operation, low cost and the like, provides a real and reliable data basis for clinical disease diagnosis, prevention and treatment, and serves inspection departments, inspection centers and the like of hospitals at all levels.
Although the semi-automatic fluorescence immunoassay analyzer in the prior art has low cost, in the detection process, reagent strips need to be manually put into the analyzer one by one for detection, only one sample can be loaded at a time, and the next sample can be loaded after the detection is finished, so that the degree of automation is low and the detection efficiency is low; meanwhile, in the manual operation process, errors are easily caused, and the detection efficiency and the detection precision are influenced.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention aims to provide a fluorescence immunoassay device and a control method, and aims to solve the problems that in the prior art, a fluorescence immunoassay instrument has a complex structure and high failure rate, so that the detection speed is not high, and the actual high-precision and high-efficiency detection requirements cannot be met.
The invention is realized by adopting the following technical scheme:
a fluorescence immunoassay device comprising: the device comprises a base, wherein an incubation module for incubating an immunological reagent at a constant temperature, at least one pushing module and at least one pushing module are arranged on the base;
the incubation module comprises a connecting rotating shaft vertically arranged on the base, the side wall of the connecting rotating shaft is rotatably connected with a conveying belt assembly for driving the connecting rotating shaft to rotate horizontally, the upper end and the lower end of the connecting rotating shaft are respectively connected with an incubation assembly and a lifting assembly, and the lifting assembly drives the connecting rotating shaft to move up and down; and a first pushing module and a second pushing module are arranged around the incubation assembly, the first pushing module is used for continuously pushing the immune reagent into the incubation assembly, and the second pushing module is used for continuously pushing the immune reagent out of the incubation assembly and detecting the immune reagent.
In order to optimize the technical scheme, the specific measures adopted further comprise:
furthermore, the lifting assembly comprises a connecting plate and a screw rod stepping motor, one end of the connecting plate is connected with a bearing between the connecting rotating shafts, and the other end of the connecting plate is rotatably connected with the screw rod stepping motor to drive the connecting plate to move up and down.
Further, it includes that the multilayer is hatched the dish and is covered the heat preservation cover of establishing on the dish is hatched to the multilayer to hatch the subassembly, hatch the dish and be connected pivot fixed connection, hatch and offer the cultivation groove that is used for placeeing the cultivation immunoreagent on the dish, demountable installation has the setting element on the cultivation groove, heat preservation cover lateral wall is provided with a plurality of heating plates.
Further, first propelling movement module includes the slip subassembly, the slip subassembly includes the first slide rail of fixed mounting on the base, sliding connection has the conveying slider on the slide rail, one side of slide rail is provided with horizontal drive's first synchronous area subassembly, first synchronous area subassembly is connected with the conveying slider, is provided with the propelling movement subassembly of propelling movement immunity reagent on the conveying slider, the base top is provided with first base plate, the spout has been seted up to first base plate, the spout top is provided with the end cover, set up the first slide that supplies immunity reagent to remove in the end cover.
Further, the propelling movement subassembly includes the fixed station, the fixed station is fixed to be set up on the conveying slider, rotate the trip that is connected with the propelling movement immunity reagent strip on the fixed station, be in vertical direction under the trip free state, the trip can be in one-way ninety degrees rotations in vertical plane.
The limiting assembly comprises a baffle plate which is fixedly arranged on the side wall of the second substrate, a vertically arranged limiting door is arranged between the baffle plate and the second substrate, an inlet is formed in the limiting door, and a pressing plate is arranged at the lower end of the limiting door; the conveying sliding block is provided with a boss, the pressing plate is in contact with the boss, and the pressing plate is pushed up and down through the back-and-forth movement of the boss, so that the first slide way is opened and closed.
Further, second propelling movement module includes the second slide rail of fixed mounting on the base, sliding connection has the card slider that moves back on the second slide rail, second synchronous band subassembly is installed to second slide rail one side, second synchronous band subassembly with move back card slider fixed connection, move back and install the objective table that the level set up and be used for placing fluorescence reagent on the card slider, install the pothook subassembly of fixed fluorescence reagent on the objective table, pothook subassembly top is provided with the second base plate with base fixed connection, the second slide that supplies fluorescence reagent to remove is seted up to the second base plate, install the setting element that is used for fixed fluorescence reagent in the second slide, install the optical detection subassembly that detects fluorescence reagent on the second base plate.
Further, the pothook subassembly includes the pivot, install triangular protrusion in the pivot, be provided with the torsional spring between arch and the pivot, the pivot alternates in the objective table both sides, the objective table both ends are seted up flutedly, the arch can be under the drive of pivot in the indentation recess.
Further, the optical detection assembly comprises a shell, a first through hole in the vertical direction is formed in the shell, a PD sensor, a first plano-convex lens, a filter lens, a spectroscope and a second plano-convex lens are sequentially arranged in the through hole from top to bottom, a second through hole in the horizontal direction is formed in one side of the mirror surface of the spectroscope, and a light source emitter is arranged in the second through hole.
A control method of a fluorescence immunoassay device, comprising the steps of:
step S1: according to the length of the first sliding rail and the length of the fluorescent reagent, the rotating speed of the first synchronous belt component and the frequency of putting in the fluorescent reagent are calculated, so that the clamping hook component can convey one fluorescent reagent at a time;
step S2: calculating the rotating speed of the conveyor belt assembly according to the time for pushing the fluorescent reagents to the incubation grooves of the incubation disc, so that when each fluorescent reagent enters the incubation disc, the next empty incubation groove of the incubation disc is aligned with the fluorescent reagent to be incubated;
step S3: the incubation disc performs constant-temperature constant-humidity incubation according to preset temperature and humidity;
step S4: and calculating the rotating speed of the second synchronous belt component according to the position of the optical detection component on the second substrate, the detection time, the length of the second substrate, the length of the fluorescent reagent and the rotating speed of the conveyor belt component, so that when the next fluorescent reagent moves to the detection position, the previous fluorescent reagent completes detection, and the next fluorescent reagent pushes the previous fluorescent reagent out of the second pushing module.
The invention has the beneficial effects that:
1. compared with the prior art, the fluorescence immunoassay device provided by the invention has the advantages that reagent strips do not need to be manually placed into the incubation device one by one, and the incubated reagent strips are manually taken out, through reasonable structural layout design, the highly automated placing and taking processes are realized, meanwhile, the structural layout of the pushing module and the pushing module is adopted, the reagent strips are pushed step by step, the stroke distance of single pushing is reduced, the pushing accuracy is improved, the pushing fault rate is reduced, the structure is simple, the occupied area is small, the working efficiency is greatly improved, and the highly automated processes of automatic pushing, automatic incubation, automatic pushing and automatic detection of a fluorescence reagent are integrally realized.
2. Compared with the prior art, the incubation module of the device has the advantages that the incubation disc which can rotate and ascend and descend through the multilayer structure can flexibly move in a three-dimensional space when the incubation groove positions are increased, the open groove position design of the incubation disc is matched, the situation that an immunoreagent is not completely aligned with a groove opening can be met, the fast insertion is realized, and the incubation efficiency is further improved.
Drawings
FIG. 1 is a schematic view of the structure of an analyzing apparatus of the present invention.
Fig. 2 is a schematic structural diagram of the first push module in fig. 1 according to the present invention.
Fig. 3 is a schematic partial structural diagram of the first push module in fig. 2 according to the present invention.
FIG. 4 is a schematic diagram of the structure of the incubation module of FIG. 1 according to the present invention.
Fig. 5 is a schematic front view of fig. 4 in accordance with the present invention.
Fig. 6 is a schematic structural diagram of the second push module in fig. 1 according to the present invention.
Fig. 7 is a schematic view of a portion of the structure of fig. 6 in accordance with the present invention.
FIG. 8 is a cross-sectional view of the optical detection assembly of FIG. 6 in accordance with the present invention.
The reference signs are: the base 10, the incubation module 20, the incubation assembly 21, the incubation tray 211, the incubation groove 2111, the positioning member 2112, the heat-insulating cover 212, the mounting member 213, the conveyor belt assembly 22, the rotary motor 221, the coupling 222, the driving pulley 223, the synchronous belt 224, the driven pulley 225, the flat key sleeve 226, the mounting seat 227, the lifting assembly 23, the connecting plate 231, the screw rod stepping motor 232, the connecting rotating shaft 24, the first pushing module 30, the first main substrate 31, the first sliding rail 32, the conveying slider 33, the first synchronous belt assembly 34, the synchronous pulley 341, the first rotating shaft 342, the first protruding block 343, the coupling 344, the driving motor 345, the tooth-shaped synchronous belt 346, the pushing assembly 35, the fixing table 351, the hook 352, the first sub-substrate 36, the end cap 37, the limiting assembly 38, the baffle 381, the limiting door 382, the pressing plate 383, the boss 384, the second pushing module 40, the second main substrate 41, the second sliding rail 42, the card-withdrawing slider 43, the first pushing module 30, the second pushing module 30, the driving module, the driving, Second synchronization tape assembly 44, stage 45, groove 451, hook assembly 46, shaft 461, protrusion 462, second sub-substrate 47, positioning member 48, optical detection assembly 49, housing 491, PD sensor 492, first plano-convex lens 493, optical filter 494, beam splitter 495, second plano-convex lens 496, light source emitter 497, and fluorescent reagent 50.
Detailed Description
Embodiments of the present invention are described in further detail below with reference to the accompanying drawings.
As shown in fig. 1, the present invention provides a fluorescence immunoassay device, which is used for performing automatic pushing, automatic incubation, automatic pushing and automatic detection processes on a fluorescence reagent, and the fluorescence immunoassay device comprises a base 10, an incubation module 20 provided with a constant temperature incubation fluorescence reagent 50 on the base 10, at least one first pushing module 30 and at least one second pushing module 40.
Referring to fig. 1-3 again, the incubation module 20 includes an incubation assembly 21, a conveyor belt assembly 22 for driving the incubation assembly 21 to rotate horizontally, a lifting assembly 23 for driving the incubation assembly 21 to move up and down, and a connecting shaft 24 for transmitting the power of the conveyor belt assembly 22 and the lifting assembly 23 to the incubation assembly 21.
The incubation assembly 21 includes an incubation tray 211 having a multi-layer structure, a plurality of incubation grooves 2111 for inserting the fluorescent reagent 50 are formed in the incubation tray 211, the incubation grooves 2111 are arranged along an outer ring of the incubation tray 211, the incubation grooves 2111 are circumferentially equidistantly distributed, and the distance between the incubation grooves 2111 is continuously arranged according to actual needs. A gap is reserved on one side of the cultivating groove 2111 close to the center of the circle of the incubation disc 211, the fluorescent reagent 50 is manually pushed out when a machine fails, the notch on one side of the cultivating groove 2111 far away from the gap is designed into a horn-shaped opening form and is used for expanding the interface into which the immunological reagent 50 is inserted, when the immunological reagent is slightly displaced due to vibration or shaking and is aligned with the notch of the cultivating groove, the immunological reagent 50 can be smoothly pushed into the cultivating groove 2111, a positioning part 2112 is detachably mounted above the cultivating groove 2111, and the positioning part 2112 is used for fixing the movement of the fluorescent reagent 50 in the vertical plane, namely, the fluorescent reagent 50 is tightly pressed into the cultivating groove 2111; be provided with heat preservation cover 212 outside incubating dish 211, a plurality of heating plates (not shown in the figure) are installed to heat preservation cover 212 inner wall, heat to the temperature that sets up through this heating plate to the air in heat preservation cover 212 to incubate fluorescence reagent 50 with constant temperature, specific temperature setting can be adjusted according to the incubation temperature that different fluorescence reagent 50 needs, does not do specific settlement in this scheme.
The center of the incubation disc 211 is provided with an installation part 213, the installation part 213 is of a cylindrical hollow cylindrical structure, and the lower end of the installation part 213 is connected with the upper end of the connecting rotating shaft 24 through a bolt; when needing to be changed and overhauled incubation disc 211, convenient to detach.
The conveyor belt assembly 22 is slidably connected between the connecting rotating shafts 24, the conveyor belt assembly 22 comprises a rotating motor 221, the rotating motor 221 is fixedly mounted on the base 10, an output shaft of the rotating motor 221 is connected with a driving pulley 223 through a coupler 222, the driving pulley 223 is connected with a driven pulley 225 through a synchronous belt 224, the size of the driven pulley 225 is larger than that of the driving pulley 223, the transmission ratio can be increased, and the transmission efficiency is improved. The connecting shaft sleeve 241 is connected to the connecting shaft sleeve 241 (namely, the connecting shaft sleeve 241 can slide up and down), the flat key sleeve 226 is installed on the outer side of the connecting shaft sleeve 241, the connecting shaft sleeve 241 and the flat key sleeve 226 are connected through a flat key, the driven pulley 225 is fixedly connected with the flat key sleeve 226 through bolts, the driven pulley 225 is used for driving the flat key sleeve 226 to horizontally rotate, bearings are installed at the upper end and the lower end of the outer side of the flat key sleeve 226, a mounting seat 227 wrapping the whole flat key sleeve 226 is arranged on the outer side of the bearing, and the flat key sleeve 226 and the mounting seat 227 can relatively rotate through the bearings. It can be understood that: the connecting shaft 24 can be driven to rotate horizontally by the rotating motor 221.
Connect 24 lower extremes of pivot and be connected with lifting unit 23, lifting unit 23 includes connecting plate 231, connecting plate 231 is connected with the lower extreme bearing of being connected pivot 24, and this connecting plate 231 and base 10 parallel arrangement are connected with lead screw step motor 232's output shaft at the opposite side of connecting plate 231, and this lead screw step motor 232 body fixed mounting can be understood on base 10: the connecting rotating shaft 24 is driven by the screw rod stepping motor 232 to move up and down, so that the incubation assembly 21 at the upper end of the connecting rotating shaft 24 is driven to move to a required position.
At least two channels are formed on the heat-insulating cover 212 of the incubation disc 211, the channels correspond to one of the incubation grooves 2111 of the incubation disc 211, and the channels are externally connected with the first pushing module 30 and the second pushing module 40 respectively.
Referring to fig. 4-5, the first pushing module 30 includes a first main substrate 31 disposed above the base 10 and fixedly connected to the base 10, a first slide rail 32 is mounted on the first main substrate 31, a conveying slider 33 is slidably connected to the first slide rail 32, a first synchronous belt assembly 34 horizontally rotating is disposed on one side of the first slide rail 32, the first synchronous belt assembly 34 is fixedly connected to the conveying slider 33, a pushing assembly 35 for pushing the fluorescent reagent 50 is disposed on the conveying slider 33, the pushing assembly 35 includes a fixing table 351, the fixing table 351 is fixedly disposed on the conveying slider 33, a hook 352 for pushing the fluorescent reagent 50 is rotatably connected to the fixing table 351, and the hook 352 can rotate in a vertical plane, and the rotation direction is opposite to the movement direction of the fluorescent reagent 50; base 10 top is provided with first vice base plate 36, the spout that supplies the propelling movement subassembly to remove is seted up to first vice base plate 36, the spout top is provided with end cover 37, set up the first slide that supplies fluorescence reagent 50 to remove in the end cover 37, the spout is connected with first slide and is link up. It can be understood that: the hook 352 is used to push the fluorescent reagent 50 to move in a single direction, and meanwhile, the hook 352 rotates in a direction opposite to the movement direction of the fluorescent reagent 50, so that the hook 352 can continuously push the fluorescent reagent 50 into the incubation groove 2111 of the incubation tray 211.
A limiting assembly 38 is further mounted on the side wall of the first substrate 35, the limiting assembly 38 includes a baffle plate 381, the baffle plate 371 is fixedly mounted on the side wall of the first sub-substrate 36, a vertically arranged limiting door 382 is mounted between the baffle plate 381 and the first sub-substrate 36, an inlet is formed in the limiting door 382, and a pressing plate 383 is arranged at the lower end of the limiting door 382; the transmission slide block 33 is provided with a boss 384, the pressing plate 383 is in contact with the boss 384, and the pressing plate 383 is pushed up and down through the back-and-forth movement of the boss 384, so that the first slide way is opened and closed. It can be understood that: the opening and closing of the first slideway by the limit door 382 realizes that only one fluorescent reagent 50 is conveyed at a time, and avoids the condition that two fluorescent reagents 50 are conveyed at the same time, which results in no incubation groove 2111 for storage.
Referring to fig. 6 to 8, the second pushing module 40 includes a second main substrate 41 fixedly mounted on the base 10, a second slide rail 42 is arranged on the second main substrate 41, a card withdrawing slide block 43 is connected on the second slide rail 42 in a sliding way, a second synchronous belt assembly 44 is arranged on one side of the second slide rail 42, the second synchronous belt assembly 44 is fixedly connected with the card withdrawing slide block 43, the card withdrawing slide block 43 is provided with a horizontally arranged object stage 45 for placing the fluorescent reagent 50, the hook assembly 46 for fixing the fluorescent reagent 50 is installed on the base, the hook assembly 46 includes a rotating shaft 461, a triangular protrusion 462 is installed on the rotating shaft 461, a torsion spring (not shown) is arranged between the protrusion 462 and the rotating shaft 461, the rotating shaft 461 is inserted into two sides of the object stage 45, two ends of the object stage 45 are provided with grooves 451, the protrusion 462 can be retracted into the groove 451 of the stage 45 under the driving of the shaft 461; be provided with the vice base plate 47 of second with base 10 fixed connection above the objective table 45, the second slide that supplies fluorescence reagent 50 to remove is seted up to second base plate 47, install the setting element 48 that is used for fixed fluorescence reagent 50 in the second slide, install the optical detection subassembly 49 that detects fluorescence reagent 50 on the second base plate 47.
The optical detection assembly 49 comprises a housing 491, wherein a first through hole in a vertical direction is formed in the housing 491, a PD sensor 492, a first plano-convex lens 493, a filter 494, a beam splitter 495 and a second plano-convex lens 496 are sequentially installed in the through hole from top to bottom, a second through hole in a horizontal direction is formed in one side of the beam splitter 495 facing the mirror surface, and a light source emitter 497 is installed in the second through hole; a 610nm band-pass filter is adopted as the optical filter 494, a 365 band-pass beam splitter is adopted as the beam splitter 495, and the angle of the beam splitter 495 is forty-five degrees; it can be understood that: 365nm laser is emitted by a light source emitter 497, reflected by a beam splitter 495 at forty-five degrees and irradiated downwards, then condensed to the surface of the fluorescent reagent 50 by a second plano-convex lens 496, then the fluorescent reagent 50 is excited to emit light of 610nm, the light is sequentially converted into parallel light by the second plano-convex lens 496, is directly irradiated by the beam splitter 495, is filtered by a light filter 494 to remove light except for 610nm, and finally is focused on a PD sensor 492 by a first plano-convex lens 493. The beam splitter 495 is arranged such that the reflected beam passes through directly due to the angle.
The first synchronous belt assembly 34 and the second synchronous belt assembly 44 have the same structure, taking the first synchronous belt assembly 34 as an example, the first synchronous belt assembly 34 includes two synchronous pulleys 341, the size of the synchronous pulleys 341 is equal, the two synchronous pulleys 341 are disposed in parallel at two ends of the same side of the first slide rail 32, and the synchronous pulleys 341 are vertically and fixedly disposed on the upper surface of the base 10, a first rotating shaft 342 is inserted in the middle of one of the synchronous pulleys 341, the first rotating shaft 342 is fixedly mounted on the base 10 through a first protrusion 343, a driving motor 345 is connected below one of the synchronous pulleys 341 through a coupling 344, a housing of the driving motor 345 is fixedly mounted on the lower surface of the base 10, and a toothed synchronous belt 346 is disposed between the synchronous pulleys 341. The toothed timing belt 346 is fixedly connected to the transfer slider 33. It can be understood that: the conveying slider 33 is driven to move back and forth by the tooth-shaped timing belt 346, and the guiding function of the moving direction is realized by the first slide rail 32.
The working principle of the invention is as follows:
firstly, the conveying sliding block 33 is moved to one end close to the limit door 382, the limit door 382 is pushed upwards by the boss 384 until the inlet of the limit door 382 is aligned with the first slide way, then the fluorescent reagent 50 is inserted into the end cover 37 from the inlet, the hook 352 is pressed to be in a horizontal state by the fluorescent reagent 50, when the fluorescent reagent 50 is completely inserted into the end cover 37, the hook 352 is in a vertical state and is positioned at the outer side of one end of the fluorescent reagent 50 close to the limit door 382, then the driving motor 345 is controlled to drive the tooth-shaped synchronous belt 346 to rotate, the tooth-shaped synchronous belt 346 drives the conveying sliding block 33 to move towards the direction of the incubation disc 211, so that the hook 352 pushes the fluorescent reagent 50 into the incubation groove 2111 in the incubation disc 211, meanwhile, due to the inclined surface structure of the boss 384, the limit door 382 moves downwards under the action of gravity, so as to seal the first slide way, it is ensured that only one fluorescent reagent 50 is conveyed at a time, and two fluorescent reagents 50 are prevented from being conveyed simultaneously, resulting in the case where no incubation groove 2111 is stored; then the driving motor 345 rotates in the reverse direction to drive the transferring slider 33 to exit the incubation slot 2111 for the next fluorescent reagent 50 to be pushed. The fluorescent reagent 50 is continuously pushed to the incubation disc 211, the automation degree is high, and the accuracy is high.
After the fluorescent reagent 50 enters the incubation groove 2111 in the incubation tray 211, the rotating motor 221 drives the incubation tray 211 to rotate horizontally once, so that the next empty incubation groove 2111 is aligned with the first pushing module to meet the next fluorescent reagent 50, and after the incubation groove 2111 of the incubation tray 211 in one layer is filled up, the screw rod stepping motor 232 drives the incubation tray 211 to move up and down, so that the incubation tray 211 with the empty incubation groove 2111 meets the new fluorescent reagent 50. The fluorescent reagent 50 is incubated in the incubation groove 2111 at a constant temperature according to a set temperature, and the incubated fluorescent reagent 50 is pushed out of the incubation tray 211 by the second pushing module 40 and is detected.
The specific process of the second pushing module 40 for pushing out the detection is as follows: the second timing belt assembly 44 is first activated, and then the second timing belt assembly 44 drives the card withdrawing slider 43 and the stage 45 to move toward the incubation tray 211, when the stage 45 is moved to a position under the fluorescent reagent 50 after completion of incubation, due to the triangular structure of the projection 462, it is pressed into the groove 451 on the stage 45 by the fluorescent reagent 50, and then the stage 45 continues to move toward the incubation tray, when the projection 462 of the stage 45 moved to the right abuts against the sidewall of the fluorescent reagent 50, i.e., the projection 462 is in the vertical state, and then the second synchronous belt assembly 44 is controlled to move reversely, the object stage 45 drives the fluorescent reagent 50 to move away from the incubation tray 211, when the fluorescent reagent 50 is moved to a position inside the second sub-substrate 47 corresponding to the optical detection assembly 49, fixed in the current position by the pressing action of the positioning member 48, and then detected by the optical detection assembly 49; while the second belt assembly 44 is again reversed to allow the next dispensing of the fluorescing agent 50. When the projection 462 pushes the next fluorescent reagent 50 to move to the detection position, the previous fluorescent reagent 50 has been detected and is completely pushed out of the present immunoassay device by the next fluorescent reagent 50. The continuous pushing out and detection of the incubated fluorescent reagent 50 from the incubation disc 211 are realized, the pushing out of the fluorescent reagent 50 and the detection of the fluorescent reagent 50 are combined through a structural device, the automation degree is high, and the detection efficiency is greatly improved.
A control method of a fluorescence immunoassay analyzer comprises the following steps:
step S1: calculating the rotating speed of the first synchronous belt component 33 and the frequency of putting in the fluorescent reagent 50 according to the length of the first slide rail 31 and the length of the fluorescent reagent 50, so that the hook components can convey one fluorescent reagent at a time;
step S2: calculating the rotating speed of the conveyor belt assembly according to the time for pushing the fluorescent reagents to the incubation grooves of the incubation disc, so that when each fluorescent reagent enters the incubation disc, the next empty incubation groove of the incubation disc is aligned with the fluorescent reagent to be incubated;
step S3: the incubation disc performs constant-temperature constant-humidity incubation according to preset temperature and humidity;
step S4: and calculating the rotating speed of the second synchronous belt component according to the position of the optical detection component on the second substrate, the detection time, the length of the second substrate, the length of the fluorescent reagent and the rotating speed of the conveyor belt component, so that when the next fluorescent reagent moves to the detection position, the previous fluorescent reagent completes detection, and the next fluorescent reagent pushes the previous fluorescent reagent out of the second pushing module.
The above is only a preferred embodiment of the present invention, and the protection scope of the present invention is not limited to the above-mentioned embodiments, and all technical solutions belonging to the idea of the present invention belong to the protection scope of the present invention. It should be noted that modifications and embellishments within the scope of the invention may be made by those skilled in the art without departing from the principle of the invention.
Claims (10)
1. A fluorescence immunoassay device, comprising: the device comprises a base, wherein an incubation module for incubating an immunological reagent at a constant temperature, at least one pushing module and at least one pushing module are arranged on the base;
the incubation module comprises a connecting rotating shaft vertically arranged on the base, the side wall of the connecting rotating shaft is rotatably connected with a conveying belt assembly for driving the connecting rotating shaft to rotate horizontally, the upper end and the lower end of the connecting rotating shaft are respectively connected with an incubation assembly and a lifting assembly, and the lifting assembly drives the connecting rotating shaft to move up and down; and a first pushing module and a second pushing module are arranged around the incubation assembly, the first pushing module is used for continuously pushing the immune reagent into the incubation assembly, and the second pushing module is used for continuously pushing the immune reagent out of the incubation assembly and detecting the immune reagent.
2. The fluoroimmunoassay device of claim 1, wherein: the lifting assembly comprises a connecting plate and a screw rod stepping motor, one end of the connecting plate is connected with a bearing between the connecting rotating shafts, and the other end of the connecting plate is rotatably connected with the screw rod stepping motor to drive the connecting plate to move up and down.
3. The fluoroimmunoassay device of claim 1, wherein: incubation subassembly includes that the multilayer is hatched the dish and is covered the heat preservation cover of establishing on the dish is hatched to the multilayer, hatch the dish and be connected pivot fixed connection, hatch and offer the cultivation groove that is used for placeeing and cultivating the immunoreagent on the dish, demountable installation has the setting element on the cultivation groove, heat preservation cover lateral wall is provided with a plurality of heating plates.
4. The fluoroimmunoassay device of claim 1, wherein: the first pushing module comprises a sliding assembly, the sliding assembly comprises a first sliding rail fixedly mounted on a base, a conveying sliding block is connected to the sliding rail in a sliding mode, a first horizontal transmission synchronous belt assembly is arranged on one side of the sliding rail and connected with the conveying sliding block, a pushing assembly used for pushing the immune reagent is arranged on the conveying sliding block, a first base plate is arranged above the base, a sliding groove is formed in the first base plate, an end cover is arranged above the sliding groove, and a first sliding way used for the immune reagent to move is formed in the end cover.
5. The fluoroimmunoassay device of claim 4, wherein: the propelling movement subassembly includes the fixed station, the fixed station is fixed to be set up on the conveying slider, it is connected with the trip of propelling movement immunity reagent strip to rotate on the fixed station, the trip is in vertical direction under the free state, the trip can be in one-way ninety degrees rotations in vertical plane.
6. The fluoroimmunoassay device of claim 4, wherein: the limiting component comprises a baffle plate which is fixedly arranged on the side wall of the second substrate, a vertically arranged limiting door is arranged between the baffle plate and the second substrate, an inlet is formed in the limiting door, and a pressing plate is arranged at the lower end of the limiting door; the conveying sliding block is provided with a boss, the pressing plate is in contact with the boss, and the pressing plate is pushed up and down through the back-and-forth movement of the boss, so that the first slide way is opened and closed.
7. The fluoroimmunoassay device of claim 1, wherein: second propelling movement module includes the second slide rail of fixed mounting on the base, sliding connection has the card slider that moves back on the second slide rail, the synchronous band subassembly of second is installed to second slide rail one side, the synchronous band subassembly of second with move back card slider fixed connection, move back and install the objective table that the level set up and be used for placing fluorescence reagent on the card slider, install the pothook subassembly of fixed fluorescence reagent on the objective table, pothook subassembly top is provided with the second base plate with base fixed connection, the second slide that supplies fluorescence reagent to remove is seted up to the second base plate, install the setting element that is used for fixed fluorescence reagent in the second slide, install the optical detection subassembly that detects fluorescence reagent on the second base plate.
8. The fluoroimmunoassay device of claim 7, wherein: the pothook subassembly includes the pivot, install triangular protrusion in the pivot, be provided with the torsional spring between arch and the pivot, the pivot alternates in the objective table both sides, the objective table both ends are seted up flutedly, the arch can be under the drive of pivot in the indentation recess.
9. The fluoroimmunoassay device of claim 7, wherein: the optical detection assembly comprises a shell, a first through hole in the vertical direction is formed in the shell, a PD sensor, a first plano-convex lens, a filter, a spectroscope and a second plano-convex lens are sequentially arranged in the through hole from top to bottom, a second through hole in the horizontal direction is formed in one side of the mirror surface of the spectroscope, and a light source emitter is arranged in the second through hole.
10. A control method of a fluorescence immunoassay analyzer comprises the following steps:
step S1: according to the length of the first sliding rail and the length of the fluorescent reagent, the rotating speed of the first synchronous belt component and the frequency of putting in the fluorescent reagent are calculated, so that the clamping hook component can convey one fluorescent reagent at a time;
step S2: calculating the rotating speed of the conveyor belt assembly according to the time for pushing the fluorescent reagents to the incubation grooves of the incubation disc, so that when each fluorescent reagent enters the incubation disc, the next empty incubation groove of the incubation disc is aligned with the fluorescent reagent to be incubated;
step S3: the incubation disc performs constant-temperature constant-humidity incubation according to preset temperature and humidity;
step S4: and calculating the rotating speed of the second synchronous belt component according to the position of the optical detection component on the second substrate, the detection time, the length of the second substrate, the length of the fluorescent reagent and the rotating speed of the conveyor belt component, so that when the next fluorescent reagent moves to the detection position, the previous fluorescent reagent completes detection, and the next fluorescent reagent pushes the previous fluorescent reagent out of the second pushing module.
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