CN219259995U

CN219259995U - Microorganism amplification device

Info

Publication number: CN219259995U
Application number: CN202320261098.0U
Authority: CN
Inventors: 吴勇; 尹焕才; 王骏; 刘子漩; 仲众; 殷建
Original assignee: Suzhou Institute of Biomedical Engineering and Technology of CAS
Current assignee: Suzhou Institute of Biomedical Engineering and Technology of CAS
Priority date: 2023-02-20
Filing date: 2023-02-20
Publication date: 2023-06-27
Anticipated expiration: 2033-02-20

Abstract

The utility model discloses a microorganism amplification device, comprising: the device comprises a base frame, a metal heat conduction seat, a heating component, a reaction tube and a heat cover. The metal heat conduction seat is fixedly arranged on the base frame, one side of the metal heat conduction seat is fixedly provided with a heating component, and the heating component is connected with a first temperature detection piece. The reaction tube is provided on the heating member, and an opening adapted to inject a sample is provided on the reaction tube. The heat cover is arranged at the opening of the reaction tube in a sealing way, and is provided with a feed inlet which is communicated with the reaction tube. The temperature rising and reducing speed of the sample is improved through the metal heat conducting seat, the heating part and the heat cover, the time of repeated high and low temperature circulation in the microorganism amplification process is shortened, and then the microorganism amplification efficiency is improved.

Description

Microorganism amplification device

Technical Field

The utility model relates to the technical field of microorganism amplification, in particular to a microorganism amplification device.

Background

Polymerase Chain Reaction (PCR) is a molecular biological technique for amplifying specific DNA fragments, which can be regarded as specific DNA replication in vitro, and the greatest feature of PCR is the ability to greatly increase minute amounts of DNA. The PCR technology is one of the most important molecular biology detection means at present, can rapidly identify microorganism types such as bacteria, fungi, viruses and the like, has relatively quick detection time and ultrahigh sensitivity, and therefore has wide application prospects in the fields of environmental science, bioscience, clinical detection, food safety and the like.

In the prior art, when microorganisms in the ocean are amplified, the microorganisms need to be sampled in the ocean in advance, then the samples are transported to a laboratory, and three basic steps of denaturation, annealing and extension are realized by changing the temperature of the samples through an amplification instrument, so that the amplification is completed. The DNA double strand is denatured and decomposed into two DNA single strands when the ambient temperature is raised to 95 ℃, the decomposed DNA single strands are combined with a primer at about 60 ℃, and DNA replication is completed under the action of DNA polymerase. Through multiple high and low temperature cycles, the DNA is amplified in large quantity. In the prior art, the temperature rising and lowering speed of the amplification instrument on a sample is low, and the microorganism amplification efficiency is low because of the need of high-low temperature circulation for many times in the amplification process.

Disclosure of Invention

Therefore, the utility model aims to solve the technical problem that the microorganism amplification efficiency is low due to the fact that the sample temperature rising and reducing speed of the amplification instrument is low in the prior art.

To this end, the present utility model proposes a microorganism amplification apparatus comprising:

a base frame;

the metal heat conduction seat is fixedly arranged on the base frame;

the heating component is fixedly arranged on one side of the metal heat conduction seat; the heating component is connected with a first temperature detection piece;

a reaction tube provided on the heating member;

the thermal cover is hermetically arranged at the opening of the reaction tube; and a feed inlet communicated with the reaction tube is formed in the hot cover.

Further, a heating groove is arranged on the heating component corresponding to the reaction tube;

the reaction tube at the end contacting the heating member is tapered.

Further, the device also comprises a sealing cover; a sealing cavity is formed in the sealing cover; the reaction tube is arranged in the sealed cavity in a sealing way.

Further, a plurality of radiating fins are fixedly arranged on the other side of the metal heat conduction seat.

Further, the device also comprises a fan, wherein the fan is fixedly arranged on the base frame; and an air outlet of the fan faces the radiating fins.

Further, the sealing cover is made of plastic materials.

Further, the heating component is a semiconductor refrigerating sheet.

Further, the device also comprises a second temperature detection piece, wherein the second temperature detection piece is electrically connected with the thermal cover.

Further, the heat cover is detachably arranged on the sealing cover.

Further, an installation groove is formed in the sealing cover corresponding to the hot cover, and an elastic piece is arranged in the installation groove.

The technical scheme of the utility model has the following advantages:

1. the microorganism amplification device provided by the utility model comprises: the device comprises a base frame, a metal heat conduction seat, a heating component, a reaction tube and a heat cover. The metal heat conduction seat is fixedly arranged on the base frame, one side of the metal heat conduction seat is fixedly provided with a heating component, and the heating component is connected with a first temperature detection piece. The reaction tube is provided on the heating member, and an opening adapted to inject a sample is provided on the reaction tube. The heat cover is arranged at the opening of the reaction tube in a sealing way, and is provided with a feed inlet which is communicated with the reaction tube.

In the microorganism amplification device, the heating component is arranged on the metal heat conduction seat, the reaction tube is arranged on the heating component, and the opening of the reaction tube is sealed by the heat cover. The hot lid is last to set up the feed inlet, and the feed inlet communicates with the reaction tube, and the operator can send into the reaction tube inside with the sample through the feed inlet, need not to dismantle the hot lid. The reaction tube is heated by the heating component, the sample in the reaction tube is further heated, the heat cover is arranged at the opening of the reaction tube in a sealing way, the heat loss in the reaction tube is prevented, the temperature rising speed of the reaction tube is improved, and the sample in the reaction tube is ensured to be in a high-temperature state rapidly. The heating component is arranged on the heat conduction metal seat, and the heat conduction metal seat is used for rapidly radiating heat, so that the heating component and the reaction tube are further enabled to rapidly radiate heat, and even if a sample in the reaction tube is rapidly lowered to a low-temperature state from a high-temperature state. The temperature rising and reducing speed of the sample is improved through the metal heat conducting seat, the heating part and the heat cover, the time of repeated high and low temperature circulation in the microorganism amplification process is shortened, and then the microorganism amplification efficiency is improved.

2. According to the microorganism amplification device provided by the utility model, the heating part is provided with the heating groove, the reaction tube can be arranged in the heating groove, one end of the reaction tube, which is contacted with the heating part, is arranged in a conical shape, and the contact area of the reaction tube and the heating part is increased by arranging the end part of the reaction tube in the conical shape, so that the heating and cooling speed of the reaction tube is increased, and the microorganism amplification efficiency is further improved.

3. The microorganism amplification device provided by the utility model further comprises a sealing cover, wherein a sealing cavity is formed in the sealing cover, and the reaction tube is arranged in the sealing cavity in a sealing way. The reaction tube is sealed by the sealing space, so that heat loss of the reaction tube to the air in the heating process is prevented, the heating speed of the reaction tube is further improved, and the microorganism amplification efficiency is improved.

4. According to the microorganism amplification device provided by the utility model, the plurality of radiating fins are fixedly arranged on the other side of the metal heat conduction seat, and the cooling speed of the reaction tube is increased by radiating the metal heat conduction seat through the radiating fins, so that the microorganism amplification efficiency is further improved.

5. The microorganism amplification device provided by the utility model further comprises a fan, wherein the fan is fixedly arranged on the base frame, the air outlet of the fan faces the direction of the radiating fins, and the radiating fins and the metal heat conduction seat are radiated through the fan, so that the cooling speed of the reaction tube is further increased, and the microorganism amplification efficiency is further improved.

6. The microorganism amplification device provided by the utility model has the advantages that the sealing cover is made of plastic material, the thermal conductivity of the plastic material is low, the heat loss of the reaction tube in the air in the heating process is prevented, the temperature rising speed of the reaction tube is further improved, and the microorganism amplification efficiency is improved.

7. The utility model provides a microorganism amplifying device, wherein a heating component is a semiconductor refrigerating sheet. The semiconductor refrigerating sheet can refrigerate and heat, and has higher heating efficiency, the temperature rising and reducing speed of the sample is improved through the semiconductor refrigerating sheet, the time of repeated high-low temperature circulation in the microorganism amplification process is shortened, and the microorganism amplification efficiency is further improved.

8. The microorganism amplification device provided by the utility model further comprises a second temperature detection part, wherein the second temperature detection part is electrically connected with the thermal cover, and the temperature on the thermal cover is detected through the second temperature detection part, so that the temperature of a sample in the reaction tube is ensured to reach a specified state.

9. The microorganism amplification device provided by the utility model has the advantages that the heat cover can be detachably arranged on the sealing cover, so that the heat cover can be conveniently detached or the maintenance reaction tube can be conveniently replaced in the later period.

10. According to the microorganism amplification device provided by the utility model, the sealing cover is provided with the mounting groove corresponding to the heat cover, the elastic piece is arranged in the mounting groove, when the heat cover is mounted, the tightness between the heat cover and the sealing cover is ensured through the elasticity exerted by the elastic piece, and meanwhile, the heat cover is prevented from crushing the sealing cover during mounting through the buffering of the elastic piece.

Drawings

In order to more clearly illustrate the embodiments of the present utility model or the technical solutions in the prior art, the drawings that are needed in the description of the embodiments or the prior art will be briefly described, and it is obvious that the drawings in the description below are some embodiments of the present utility model, and other drawings can be obtained according to the drawings without inventive effort for a person skilled in the art.

FIG. 1 is a schematic diagram showing the structure of a microorganism amplifying apparatus according to example 1 of the present utility model;

FIG. 2 is a schematic view showing the structure of a hidden seal cover of the microorganism amplifying device in example 1 of the present utility model;

FIG. 3 is a schematic diagram showing the structure of the heat cover and the reaction tube in the microorganism amplifying apparatus according to example 1 of the present utility model.

Reference numerals illustrate:

1. a base frame; 2. a metal heat conduction seat; 3. a sealing cover; 4. a thermal cover; 5. a feed pipe; 6. a first temperature detecting wire; 7. a second temperature detecting wire; 8. a temperature control wire; 9. a heat radiation fin; 10. a blower; 11. a heating member; 12. a reaction tube.

Detailed Description

The following description of the embodiments of the present utility model will be made apparent and fully in view of the accompanying drawings, in which some, but not all embodiments of the utility model are shown. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

In the description of the present utility model, it should be noted that the directions or positional relationships indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. are based on the directions or positional relationships shown in the drawings, are merely for convenience of describing the present utility model and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present utility model. Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present utility model, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present utility model will be understood in specific cases by those of ordinary skill in the art.

In addition, the technical features of the different embodiments of the present utility model described below may be combined with each other as long as they do not collide with each other.

Example 1

The present embodiment provides a microorganism amplification apparatus, as shown in fig. 1 and 2, comprising: the heat conduction device comprises a base frame 1, a metal heat conduction seat 2, a heating component 11, a reaction tube 12 and a heat cover 4. The metal heat conduction seat 2 is fixedly arranged on the base frame 1, a heating component 11 is fixedly arranged on one side of the metal heat conduction seat 2, and a first temperature detection piece is connected to the heating component 11. For example: the first temperature detecting member is connected to the heating member 11 through the second temperature detecting wire 7, and the heating temperature of the heating member 11 is detected by the first temperature detecting member. The heating part 11 is provided with a temperature control wire 8, and the temperature control wire 8 is connected with a controller so as to control the temperature of the heating part 11. A reaction tube 12 is provided on the heating member 11, and an opening adapted to inject a sample is provided on the reaction tube 12. The heat cover 4 is arranged at the opening of the reaction tube 12 in a sealing way, a feed inlet is arranged on the heat cover 4, one end of the feed inlet is communicated with the reaction tube 12, and the other end is communicated with the feed tube 5.

In the microorganism amplification device, a heating member 11 is provided on a metal heat conduction seat 2, a reaction tube 12 is provided on the heating member 11, and an opening of the reaction tube 12 is sealed by a heat cover 4. The thermal cover 4 is provided with a feed inlet which is communicated with the reaction tube 12, and an operator can send a sample into the reaction tube 12 through the feed inlet without disassembling the thermal cover 4. The reaction tube 12 is heated by the heating component 11, the sample in the reaction tube 12 is further heated, the thermal cover 4 is arranged at the opening of the reaction tube 12 in a sealing way, so that the heat loss in the reaction tube 12 is prevented, the temperature rising speed of the reaction tube 12 is improved, and the sample in the reaction tube 12 can be ensured to reach a high temperature state rapidly. The heating component 11 is arranged on the heat conducting metal seat, and the heat is quickly dissipated through the heat conducting metal seat, so that the heating component 11 and the reaction tube 12 are further quickly dissipated, namely, the sample in the reaction tube 12 is quickly lowered from a high-temperature state to a low-temperature state. The temperature rising and reducing speed of the sample is improved through the metal heat conduction seat 2, the heating part 11 and the heat cover 4, the time of repeated high and low temperature circulation in the microorganism amplification process is shortened, and the microorganism amplification efficiency is further improved. Meanwhile, the microorganism amplification device can be directly arranged on a ship, namely, the microorganism amplification can be carried out on the ship by sampling in the ocean, and the sample is not required to be transported to a laboratory for the amplification experiment. The reaction tube 12 is sealed by the heat cover 4, so that the internal temperature of the reagent tube is uniform, the temperature gradient is avoided to accelerate the evaporation of liquid, the adhesion between the reaction tube 12 and the heating component 11 is improved, the heat conduction is enhanced, and the temperature rising and falling speed of a sample is improved. Can realize the in-situ rapid amplification of the marine microorganism nucleic acid.

Specifically, as shown in fig. 3, the heating component 11 is provided with a heating groove, the reaction tube 12 may be disposed in the heating groove, one end of the reaction tube 12 in contact with the heating component 11 is provided with a conical shape, and the end of the reaction tube 12 is provided with a conical shape, so that the contact area between the reaction tube 12 and the heating component 11 is increased, and further the heating and cooling speeds of the reaction tube 12 are increased, and the microorganism amplification efficiency is further improved. The end of the reaction tube 12 may be further provided in an arc shape, and the contact area between the reaction tube 12 and the heating part 11 may be increased by the arc shape.

Specifically, as shown in fig. 1, the reactor further comprises a sealing cover 3, a sealing cavity is formed in the sealing cover 3, and the reaction tube 12 is arranged in the sealing cavity in a sealing mode. The reaction tube 12 is sealed by the sealing space, so that heat loss of the reaction tube 12 to the air in the heating process is prevented, the heating speed of the reaction tube 12 is further improved, and the microorganism amplification efficiency is improved. The sealing cover 3 may be fixedly arranged on the base frame 1, or the sealing cover 3 may also be fixedly arranged on the metal heat conduction seat 2. For example: as shown in fig. 2, the metal heat conduction seat 2 has a plate shape, the seal cover 3 is fixedly provided on the metal heat conduction seat 2, and the seal cover 3 is covered on the heating member 11.

Specifically, as shown in fig. 1 and fig. 2, a plurality of heat dissipation fins 9 are fixedly arranged on the other side of the metal heat conduction seat 2, and the heat dissipation fins 9 are arranged on the metal heat conduction seat 2 at intervals. It will be appreciated that the heat radiating fins 9 and the heating member 11 are provided on both sides of the metal heat conduction seat 2, respectively. The cooling speed of the reaction tube 12 is increased by radiating the metal heat conduction seat 2 through the radiating fins 9, so that the microorganism amplification efficiency is further improved.

Specifically, as shown in fig. 2, the device further comprises a fan 10, the fan 10 is fixedly arranged on the base frame 1, an air outlet of the fan 10 faces to the direction of the radiating fins 9, the radiating fins 9 and the metal heat conduction seat 2 are radiated through the fan 10, the cooling speed of the reaction tube 12 is further increased, and the microorganism amplification efficiency is further improved.

Specifically, the sealing cover 3 is made of plastic, the thermal conductivity of the plastic is low, heat loss of the reaction tube 12 to the air in the heating process is prevented, the heating speed of the reaction tube 12 is further improved, and the microorganism amplification efficiency is improved.

Specifically, the heating member 11 is a semiconductor refrigeration sheet. The semiconductor refrigerating sheet can refrigerate and heat, and has higher heating efficiency, the temperature rising and reducing speed of the sample is improved through the semiconductor refrigerating sheet, the time of repeated high-low temperature circulation in the microorganism amplification process is shortened, and the microorganism amplification efficiency is further improved.

Specifically, as shown in fig. 1 and 2, the device further comprises a second temperature detecting member, and the second temperature detecting member is electrically connected with the thermal cover 4. The heat cover 4 may be provided with a first temperature detecting wire 6, and the first temperature detecting wire 6 is connected with a second temperature detecting member. The temperature on the thermal cover 4 is detected by the second temperature detecting member, so that the temperature of the sample in the reaction tube 12 is ensured to reach a specified state.

Specifically, as shown in fig. 1, the heat cover 4 is detachably provided on the sealing cover 3, so that the heat cover 4 can be detached or the maintenance reaction tube 12 can be replaced at a later stage. For example, the heat cover 4 may be detachably provided on the seal cover 3 by a connector such as a bolt, or the heat cover 4 and the seal cover 3 may be detachably connected by a snap.

Specifically, the sealing cover 3 is provided with a mounting groove corresponding to the heat cover 4, and an elastic piece is arranged in the mounting groove and can be a spring. When the heat cover 4 is installed, the tightness between the heat cover 4 and the seal cover 3 is ensured by the elasticity applied by the elastic member, and the heat cover 4 is prevented from crushing the seal cover 3 when installed by the buffering of the elastic member.

Specifically, an Active Disturbance Rejection Control (ADRC) technique may be employed to achieve precise control of temperature. ADRC has strong anti-interference capability, fast control response, no overshoot and static error, good robustness and stability, wide parameter adjustment range and better control quality than classical PID.

The working principle of the microorganism amplification device is as follows:

the heat is uniformly transferred to the reaction tube 12 through the heating part 11. The temperature of the sample can be indirectly changed by controlling the change of the heat source power and the continuous acting of the fan 10, so that three basic steps of denaturation, annealing and extension are realized, and the amplification of microorganisms is completed. The reaction tube 12 is heated by the heating component 11, the sample in the reaction tube 12 is further heated, the thermal cover 4 is arranged at the opening of the reaction tube 12 in a sealing way, so that the heat loss in the reaction tube 12 is prevented, the temperature rising speed of the reaction tube 12 is improved, and the sample in the reaction tube 12 can be ensured to reach a high temperature state rapidly. The heating component 11 is arranged on the heat conducting metal seat, and the heat is quickly dissipated through the heat conducting metal seat, so that the heating component 11 and the reaction tube 12 are further quickly dissipated, namely, the sample in the reaction tube 12 is quickly lowered from a high-temperature state to a low-temperature state. The temperature rising and reducing speed of the sample is improved through the metal heat conduction seat 2, the heating part 11 and the heat cover 4, the time of repeated high and low temperature circulation in the microorganism amplification process is shortened, and the microorganism amplification efficiency is further improved. The device pre-denaturation temperature can be 93 ℃ and is maintained for 2 minutes; then nucleic acid denaturation was performed, with the procedure at 93℃for 6 seconds; then annealing operation is carried out, and the extension procedure is that the temperature is 60 ℃ for 30 seconds; finally, the denaturation program and the annealing program are sequentially subjected to 40 cycles to complete the PCR amplification process.

It is apparent that the above examples are given by way of illustration only and are not limiting of the embodiments. Other variations or modifications of the above teachings will be apparent to those of ordinary skill in the art. It is not necessary here nor is it exhaustive of all embodiments. While still being apparent from variations or modifications that may be made by those skilled in the art are within the scope of the utility model.

Claims

1. A microorganism amplification apparatus comprising:

a base frame (1);

the metal heat conduction seat (2) is fixedly arranged on the base frame (1);

the heating component (11) is fixedly arranged on one side of the metal heat conduction seat (2); the heating component (11) is connected with a first temperature detection piece;

a reaction tube (12) provided on the heating member (11);

a thermal cover (4) which is arranged at the opening of the reaction tube (12) in a sealing way; the hot cover (4) is provided with a feed inlet communicated with the reaction tube (12).

2. The microorganism amplification apparatus according to claim 1, wherein a heating groove is provided on the heating member (11) corresponding to the reaction tube (12);

the reaction tube (12) at the end contacting the heating member (11) is provided in a tapered shape.

3. The microorganism amplification apparatus according to claim 2, further comprising a sealing cap (3); a sealing cavity is formed in the sealing cover (3); the reaction tube (12) is arranged in the sealed cavity in a sealing way.

4. A microorganism amplifying device according to claim 3, wherein a plurality of heat radiating fins (9) are fixedly arranged on the other side of the metal heat conducting base (2).

5. The microorganism amplification apparatus according to claim 4, further comprising a blower (10), the blower (10) being fixedly provided on the base frame (1); the air outlet of the fan (10) faces the radiating fins (9).

6. A microorganism amplifying device according to claim 3, wherein the sealing cover (3) is of plastics material.

7. A microorganism amplifying device according to any one of claims 1 to 6, wherein the heating means (11) is a semiconductor cooling plate.

8. The microorganism amplification apparatus according to any one of claims 1 to 6, further comprising a second temperature detection member electrically connected to the thermal cover (4).

9. A microbial amplification device according to any one of claims 3 to 6, wherein the thermal cover (4) is detachably provided on the sealing cap (3).

10. The microorganism amplification device according to claim 9, wherein the sealing cap (3) is provided with a mounting groove corresponding to the thermal cover (4), and an elastic member is provided in the mounting groove.