CN215050299U - PCR augmentor with forced induction - Google Patents
PCR augmentor with forced induction Download PDFInfo
- Publication number
- CN215050299U CN215050299U CN202022283444.4U CN202022283444U CN215050299U CN 215050299 U CN215050299 U CN 215050299U CN 202022283444 U CN202022283444 U CN 202022283444U CN 215050299 U CN215050299 U CN 215050299U
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- plate
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- metal plate
- heating
- pcr amplification
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- 230000006698 induction Effects 0.000 title claims abstract description 11
- 239000002184 metal Substances 0.000 claims abstract description 41
- 238000010438 heat treatment Methods 0.000 claims abstract description 40
- 238000007789 sealing Methods 0.000 claims abstract description 32
- 238000012408 PCR amplification Methods 0.000 claims abstract description 28
- 238000001816 cooling Methods 0.000 claims description 4
- 230000000694 effects Effects 0.000 abstract description 5
- 230000003321 amplification Effects 0.000 abstract description 4
- 238000003199 nucleic acid amplification method Methods 0.000 abstract description 4
- 238000003752 polymerase chain reaction Methods 0.000 description 11
- 238000005057 refrigeration Methods 0.000 description 4
- 238000000935 solvent evaporation Methods 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- 238000004321 preservation Methods 0.000 description 3
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 2
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000017525 heat dissipation Effects 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 239000012774 insulation material Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000004543 DNA replication Effects 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 150000002972 pentoses Chemical class 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000004153 renaturation Methods 0.000 description 1
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Abstract
The utility model discloses a PCR amplification instrument with pressure induction, which comprises a temperature control component and a heat cover component, wherein the temperature control component comprises a first supporting plate, a first metal plate is arranged on the first supporting plate, a plurality of heating chambers which are upwards opened and are matched with microporous plates are arranged on the first metal plate, and a first heating and refrigerating plate and a first radiating fin are arranged below the first metal plate; the hot cover component can move in the vertical direction and is used for covering the microporous plate and comprises a second supporting plate, a second metal plate is fixedly arranged on the second supporting plate, a downward groove is formed in the second metal plate, a sealing gasket is arranged in the groove and matched with the microporous plate, and a second heating and refrigerating plate and a second radiating fin are arranged above the second metal plate; one side of the hot cover component is provided with a pressure sensor for detecting the pressure between the sealing gasket and the microporous plate. The utility model discloses it is sealed with the sample amplification to integrate the micropore board to the inseparable degree between convenient adjustment sealed pad and the micropore board reaches the sealed effect of ideal.
Description
Technical Field
The utility model relates to a PCR augmentor with forced induction belongs to PCR equipment technical field.
Background
The Polymerase Chain Reaction (PCR) is a molecular biology technology for amplifying and amplifying specific DNA fragments, which can be regarded as special DNA replication in vitro, and the biggest characteristic of the PCR is that trace DNA can be greatly increased, wherein the DNA is denatured at high temperature of 95 ℃ in vitro and becomes single-stranded, a primer and the single-stranded are combined according to the principle of base complementary pairing at low temperature (usually about 60 ℃), the temperature is adjusted to the optimal reaction temperature (about 72 ℃) of the DNA polymerase, and the DNA polymerase synthesizes a complementary strand along the direction from phosphoric acid to pentose (5 '-3'). The PCR instrument manufactured based on polymerase is actually a temperature control device, and can be well controlled among denaturation temperature, renaturation temperature and extension temperature.
Before PCR reaction, in order to prevent sample reagents from being volatilized by heating and cross contamination among samples, a sealing film is often sealed at the upper end of the microplate to seal the sample reagents in respective reaction systems. Usually seal the membrane with the micropore board with the membrane sealing machine earlier, then put and carry out the amplification reaction on the PCR appearance, this just needs two kinds of equipment of purchase membrane sealing machine and PCR appearance simultaneously, has not only increased the cost of experiment operation, has increased the step of experiment and the space that equipment occupy simultaneously, causes the inconvenience for the experimenter.
SUMMERY OF THE UTILITY MODEL
In order to solve the problems, the utility model provides a PCR amplification instrument with pressure induction, which effectively integrates the functions of micropore plate sealing and sample amplification, and can simultaneously carry out real-time induction feedback on the pressure between a hot cover and a micropore plate, so that experimenters can conveniently adjust the tightness between a sealing gasket and the micropore plate, and an ideal sealing effect is achieved; the PCR amplification instrument is small in design volume, can be used independently, and can be effectively integrated with a pipetting workstation, so that the multifunctional requirement of the pipetting workstation is met.
The first objective of the present invention is to provide a PCR amplification apparatus with pressure sensing, which includes:
the temperature control assembly comprises a first supporting plate, a first metal plate is arranged on the first supporting plate, a plurality of heating chambers with upward openings are arranged on the first metal plate, the openings are matched with the microporous plates, a first heating and refrigerating plate is arranged below the first metal plate, and first cooling fins are arranged below the first heating and refrigerating plate; and the number of the first and second groups,
the heat cover assembly can move in the vertical direction and is used for covering a microporous plate arranged on the temperature control assembly, the heat cover assembly comprises a second supporting plate, a second metal plate is fixedly arranged on the second supporting plate, a downward groove is formed in the second metal plate, a sealing gasket is arranged in the groove and matched with the microporous plate, a second heating and refrigerating plate is arranged above the second metal plate, and a second radiating fin is arranged above the second heating and refrigerating plate; and one side of the hot cover component is also provided with a pressure sensor for detecting the pressure between the sealing gasket and the microporous plate.
Further, the hot cap subassembly drive through the vertical drive subassembly, the vertical drive subassembly includes the second motor, is used for the second motor support of fixed second motor, the second screw rod that links to each other with the second motor to and the second guide that links to each other with the second motor support, the second screw rod other end and second backup pad threaded connection, second guide and second screw rod parallel arrangement wear to establish the second backup pad.
Further, the temperature control assembly can move in the horizontal direction and drive through the horizontal driving assembly, the horizontal driving assembly comprises a first motor, a first motor support used for fixing the first motor, a first screw rod connected with the first motor and a first guide piece connected with the first motor support, the other end of the first screw rod is in threaded connection with the first supporting plate, and the first guide piece and the first screw rod are arranged in parallel and penetrate through the first supporting plate.
Further, the first guide assembly or the second guide member comprises two guide posts and two guide sleeves.
Furthermore, the side of temperature control component be equipped with first temperature sensor, the side of hot lid subassembly is equipped with second temperature sensor. Respectively for detecting the temperature of the first metal plate and the second metal plate.
Further, the PCR amplification instrument further comprises a main control board, and the main control board is respectively electrically connected with the first heating and refrigerating board, the second heating and refrigerating board, the first motor, the second motor, the pressure sensor, the first temperature sensor and the second temperature sensor.
Furthermore, a plurality of through holes are arranged in the groove of the second metal plate.
Furthermore, one side of the sealing gasket is provided with a plurality of blind holes, and the blind holes correspond to the through holes and correspond to the micro-porous plate.
Further, the sealing gasket is made of high-temperature-resistant heat conduction materials.
In the invention, one side of the sealing gasket is provided with the blind hole, but the front side and the back side of the sealing gasket are not required to be distinguished in the use process, when the blind hole faces the microporous plate, the sealing area of the blind hole can be increased, and the volume cavity formed between the bottom of the blind hole and the micropores of the microporous plate is increased, so that the vapor pressure formed by solvent evaporation can be resisted in the heating process, and the mutual interference among different micropores caused by insufficient applied pressure can not be caused; when the blind hole is opposite to the microporous plate, the bottom of the blind hole is thinner, so that the bottom of the blind hole bulges in the blind hole due to vapor pressure in the heating process, the vapor pressure formed by solvent evaporation can be tolerated, and mutual interference between micropores due to insufficient applied pressure cannot be caused.
The PCR amplification instrument further comprises a shell, wherein a first bin door and a second bin door are arranged on the shell, and the first bin door is arranged corresponding to the temperature control component and used for entering and exiting the temperature control component; the second bin door is arranged corresponding to the heat cover assembly and used for taking and placing the sealing gasket.
Further, the bottom of the shell is also provided with a radiator. The heat dissipation of the equipment is accelerated by arranging the radiator.
Furthermore, heat preservation and insulation materials are arranged around the first heating and refrigerating plate, the second heating and refrigerating plate, the first metal plate and the second metal plate. Is used for reducing heat loss and improving the heat preservation effect.
The utility model has the advantages that:
the utility model discloses a PCR amplification appearance with forced induction, sealed and the sample amplification function of micropore board carries out effective integration, can carry out real-time response feedback to the pressure between hot lid and the micropore board simultaneously to make things convenient for the experimenter to adjust the inseparability between sealed pad and the micropore board, thereby reach ideal sealed effect; the PCR amplification instrument is small in design volume, can be used independently, and can be effectively integrated with a pipetting workstation, so that the multifunctional requirement of the pipetting workstation is met.
Drawings
FIG. 1 is an overall side view of a PCR amplification apparatus of the present invention;
FIG. 2 is a back sectional view of the PCR amplification apparatus of the present invention;
FIG. 3 is a side sectional view of the PCR amplification apparatus of the present invention;
FIG. 4 is a bottom view of the PCR amplification apparatus of the present invention;
FIG. 5 is a side sectional view of a temperature control assembly of the PCR amplification apparatus of the present invention;
FIG. 6 is an overall view of the heat cap assembly of the PCR amplification apparatus of the present invention;
FIG. 7 is a schematic view of a second metal plate of the PCR amplification apparatus of the present invention;
FIG. 8 is a schematic view of a gasket of the PCR amplification apparatus of the present invention.
Wherein the reference numbers indicate: 1. the temperature control assembly, 11, the first support plate, 12, the first metal plate, 13, the heating chamber, 14, the first heating and refrigerating plate, 15, the first heat sink, 16, the first temperature sensor, 2, the heat sink assembly, 21, the second support plate, 22, the second metal plate, 23, the groove, 24, the sealing gasket, 25, the second heating and refrigerating plate, 26, the second heat sink, 27, the pressure sensor, 28, the second temperature sensor, 31, the second motor, 32, the second motor support, 33, the second screw, 34, the second guide, 41, the first motor, 42, the first motor support, 43, the first screw, 44, the first guide, 5, the main control plate, 6, the housing, 61, the first door, 62, the second door, 63, and the radiator.
Detailed Description
The present invention is further described with reference to the following drawings and specific embodiments so that those skilled in the art can better understand the present invention and can implement the present invention, but the embodiments are not to be construed as limiting the present invention.
Example 1:
as shown in fig. 1-8, the PCR amplification apparatus with pressure sensing of the present invention comprises:
the temperature control assembly 1 comprises a first supporting plate 11, a first metal plate 12 is arranged on the first supporting plate 11, a plurality of heating chambers 13 with upward openings are arranged on the first metal plate 12, the openings are matched with microporous plates, a first heating and refrigerating plate 14 is arranged below the first metal plate 12, and first radiating fins 15 are arranged below the first heating and refrigerating plate 14; and the number of the first and second groups,
the heat cover component 2 can move in the vertical direction and is used for covering a microporous plate arranged on the temperature control component 1, the heat cover component 2 comprises a second supporting plate 21, a second metal plate 22 is fixedly arranged on the second supporting plate 21, a downward groove 23 is formed in the second metal plate 22, a sealing gasket 24 is arranged in the groove 23, the sealing gasket 24 is matched with the microporous plate, a second heating and refrigerating plate 25 is arranged above the second metal plate 22, and second radiating fins 26 are arranged above the second heating and refrigerating plate 25; the hot lid assembly 2 is further provided with a pressure sensor 27 at one side thereof for detecting a pressure between the gasket 24 and the micro plate.
The utility model discloses a hot lid subassembly 2 drives through the vertical drive subassembly, and the vertical drive subassembly includes second motor 31, is used for fixed second motor 31's second motor support 32, the second screw rod 33 that links to each other with second motor 31 to and the second guide 34 that links to each other with second motor support 32, the second screw rod 33 other end and the 21 threaded connection of second backup pad, second guide 34 and second screw rod 33 parallel arrangement wear to establish second backup pad 21. Wherein, the second guide assembly includes two guide posts and two guide sleeves.
The utility model discloses a temperature control subassembly 1 can remove at the horizontal direction, drive through the horizontal drive subassembly, the horizontal drive subassembly includes first motor 41, a first motor support 42 for fixing first motor 41, the first screw rod 43 that links to each other with first motor 41 to and the first guide 44 that links to each other with first motor support 42, the first screw rod 43 other end and 11 threaded connection of first backup pad, first guide 44 and first screw rod 43 parallel arrangement wear to establish first backup pad 11. Wherein, the first guide part comprises two guide posts and two guide sleeves.
The utility model discloses a side of temperature control component 1 is equipped with first temperature sensor 16, and the side of hot lid subassembly 2 is equipped with second temperature sensor 28. Respectively for detecting the temperature of the first metal plate and the second metal plate.
The utility model discloses a PCR amplificator still includes main control board 5, and main control board 5 is connected with first heating refrigeration board 14, second heating refrigeration board 25, first motor 41, second motor 31, pressure sensor 27, first temperature sensor 16, the 28 electricity of second temperature sensor respectively.
As shown in fig. 7, a plurality of through holes are provided in the groove 23 of the second metal plate 22 of the present invention. As shown in fig. 8, the sealing pad 24 of the present invention is provided with a plurality of blind holes corresponding to the through holes in the grooves and corresponding to the micro-porous plates. The utility model discloses a sealed pad is obtained by the preparation of high temperature resistant heat conduction material.
In the invention, one side of the sealing gasket is provided with the blind hole, but the front side and the back side of the sealing gasket are not required to be distinguished in the use process, when the blind hole faces the microporous plate, the sealing area of the blind hole can be increased, and the volume cavity formed between the bottom of the blind hole and the micropores of the microporous plate is increased, so that the vapor pressure formed by solvent evaporation can be resisted in the heating process, and the mutual interference among different micropores caused by insufficient applied pressure can not be caused; when the blind hole is opposite to the microporous plate, the bottom of the blind hole is thinner, so that the bottom of the blind hole bulges in the blind hole due to vapor pressure in the heating process, the vapor pressure formed by solvent evaporation can be tolerated, and mutual interference between micropores due to insufficient applied pressure cannot be caused.
The PCR amplification instrument of the utility model also comprises a shell 6, a first bin gate 61 and a second bin gate 62 are arranged on the shell 6, the first bin gate 61 is arranged corresponding to the temperature control component 1 and is used for the entering and exiting of the temperature control component 1; the second door 62 is disposed corresponding to the heat cover assembly 2 for taking and placing the gasket 24. The bottom of the housing 6 is also provided with a heat sink 63. The heat dissipation of the equipment is accelerated by arranging the radiator.
Furthermore, the utility model discloses a first heating refrigeration board, second heating refrigeration board, first metal sheet, second metal sheet are equipped with thermal insulation material, not shown in the figure around. Is used for reducing heat loss and improving the heat preservation effect.
The utility model discloses a theory of operation: when a sample needs to be subjected to PCR amplification, firstly, a layer of gum is arranged on one surface, in contact with the second metal plate, of the sealing gasket in advance, the sealing gasket is attached to the groove of the second metal plate through the second bin gate, the front side and the back side of the sealing gasket do not need to be distinguished, the microporous plate containing a sample reagent is placed in the columnar heating chamber above the first metal plate in an aligned mode, the main control board controls the first motor to rotate, the temperature control assembly is driven to return to the bin through the first screw rod, the main control board controls the second motor to rotate, the heat cover assembly is driven to vertically move downwards through the second screw rod, the pressure sensor detects the pressure between the sealing gasket and the microporous plate in real time, when the pressure reaches a preset value, the second motor stops rotating, the main control board controls the first heating and refrigerating plate and the second heating and refrigerating plate to respectively carry out heating or refrigerating according to a preset temperature control program, and controls the first temperature sensor and the second temperature sensor simultaneously, The radiator starts to work; after the temperature control is finished, the main control board controls the second motor to rotate, the second screw rod drives the hot cover component to vertically move upwards, then the main control board controls the first motor to rotate, the first screw rod drives the temperature control component to leave the warehouse, and finally the microporous plate and the sealing gasket are manually taken out.
The above-mentioned embodiments are merely preferred embodiments for fully illustrating the present invention, and the scope of the present invention is not limited thereto. Equivalent substitutes or changes made by the technical personnel in the technical field on the basis of the utility model are all within the protection scope of the utility model. The protection scope of the present invention is subject to the claims.
Claims (10)
1. A PCR amplification instrument with pressure induction is characterized by comprising:
the temperature control assembly comprises a first supporting plate, a first metal plate is arranged on the first supporting plate, a plurality of heating chambers with upward openings are arranged on the first metal plate, the openings are matched with the microporous plates, a first heating and refrigerating plate is arranged below the first metal plate, and first cooling fins are arranged below the first heating and refrigerating plate; and the number of the first and second groups,
the heat cover assembly can move in the vertical direction and is used for covering a microporous plate arranged on the temperature control assembly, the heat cover assembly comprises a second supporting plate, a second metal plate is fixedly arranged on the second supporting plate, a downward groove is formed in the second metal plate, a sealing gasket is arranged in the groove and matched with the microporous plate, a second heating and refrigerating plate is arranged above the second metal plate, and a second radiating fin is arranged above the second heating and refrigerating plate; and one side of the hot cover component is also provided with a pressure sensor for detecting the pressure between the sealing gasket and the microporous plate.
2. The PCR amplification instrument with pressure induction of claim 1, wherein the heat cover assembly is driven by a vertical driving assembly, the vertical driving assembly comprises a second motor, a second motor bracket for fixing the second motor, a second screw rod connected with the second motor, and a second guide member connected with the second motor bracket, the other end of the second screw rod is in threaded connection with the second support plate, and the second guide member is arranged in parallel with the second screw rod and penetrates through the second support plate.
3. The PCR amplification apparatus with pressure sensing of claim 1, wherein the temperature control assembly is movable in a horizontal direction and driven by a horizontal driving assembly, the horizontal driving assembly comprises a first motor, a first motor bracket for fixing the first motor, a first screw connected to the first motor, and a first guide connected to the first motor bracket, the other end of the first screw is connected to the first support plate by a screw thread, and the first guide is parallel to the first screw and penetrates through the first support plate.
4. The PCR amplification apparatus with pressure sensing of claim 2, wherein the second guide member comprises two guide posts and two guide sleeves.
5. The PCR amplification apparatus with pressure sensing of claim 1, wherein the temperature control module has a first temperature sensor at a side thereof, and the heat cover module has a second temperature sensor at a side thereof.
6. The PCR amplification instrument with pressure induction of claim 5, further comprising a main control board, wherein the main control board is electrically connected to the first heating and cooling board, the second heating and cooling board, the first motor, the second motor, the pressure sensor, the first temperature sensor and the second temperature sensor, respectively.
7. The PCR amplification apparatus with pressure sensing of claim 1, wherein the second metal plate has a plurality of through holes in the recess.
8. The PCR amplification apparatus with pressure sensing of claim 7, wherein the sealing pad has a plurality of blind holes on one side thereof, the blind holes corresponding to the through holes and corresponding to the micro-porous plate.
9. The PCR amplification instrument with pressure induction of claim 1, further comprising a housing, wherein the housing is provided with a first door and a second door, the first door is arranged corresponding to the temperature control assembly and is used for entering and exiting the temperature control assembly; the second bin door is arranged corresponding to the heat cover assembly and used for taking and placing the sealing gasket.
10. The PCR amplifier with pressure sensing of claim 9, wherein the bottom of the housing further comprises a heat sink.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022283444.4U CN215050299U (en) | 2020-10-14 | 2020-10-14 | PCR augmentor with forced induction |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022283444.4U CN215050299U (en) | 2020-10-14 | 2020-10-14 | PCR augmentor with forced induction |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN215050299U true CN215050299U (en) | 2021-12-07 |
Family
ID=79159955
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202022283444.4U Expired - Fee Related CN215050299U (en) | 2020-10-14 | 2020-10-14 | PCR augmentor with forced induction |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN215050299U (en) |
-
2020
- 2020-10-14 CN CN202022283444.4U patent/CN215050299U/en not_active Expired - Fee Related
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| Date | Code | Title | Description |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20211207 |