CN219861309U - Thermal cycling device of polymerase chain reaction analyzer - Google Patents
Thermal cycling device of polymerase chain reaction analyzer Download PDFInfo
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- CN219861309U CN219861309U CN202320909519.6U CN202320909519U CN219861309U CN 219861309 U CN219861309 U CN 219861309U CN 202320909519 U CN202320909519 U CN 202320909519U CN 219861309 U CN219861309 U CN 219861309U
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- 238000005382 thermal cycling Methods 0.000 title claims abstract description 48
- 238000003752 polymerase chain reaction Methods 0.000 title claims abstract description 35
- 239000012530 fluid Substances 0.000 claims abstract description 26
- 238000003860 storage Methods 0.000 claims abstract description 22
- 239000013529 heat transfer fluid Substances 0.000 claims abstract description 21
- 230000001351 cycling effect Effects 0.000 claims abstract description 11
- 238000009792 diffusion process Methods 0.000 claims abstract description 4
- 238000009413 insulation Methods 0.000 claims abstract description 4
- 238000001816 cooling Methods 0.000 claims description 57
- 239000004065 semiconductor Substances 0.000 claims description 26
- 239000007788 liquid Substances 0.000 claims description 20
- 238000010438 heat treatment Methods 0.000 claims description 18
- 239000013307 optical fiber Substances 0.000 claims description 18
- 239000000463 material Substances 0.000 claims description 10
- 230000000694 effects Effects 0.000 claims description 7
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 5
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 claims description 5
- 229910052782 aluminium Inorganic materials 0.000 claims description 5
- 229910052802 copper Inorganic materials 0.000 claims description 5
- 239000010949 copper Substances 0.000 claims description 5
- 230000000630 rising effect Effects 0.000 claims description 4
- 230000003139 buffering effect Effects 0.000 claims description 3
- 238000006243 chemical reaction Methods 0.000 description 16
- 230000009286 beneficial effect Effects 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 230000007613 environmental effect Effects 0.000 description 4
- 230000006872 improvement Effects 0.000 description 4
- 238000009434 installation Methods 0.000 description 3
- 230000008859 change Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000005096 rolling process Methods 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 238000004093 laser heating Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
Abstract
The utility model provides a thermal cycling device of a polymerase chain reaction analyzer, which belongs to the technical field of experimental consumables, and comprises a thermal cycling shell, a temperature control instrument, a thermal cycling shell cover, a sample storage container, a thermal fluid cycling assembly and a controller, wherein the thermal cycling shell is of a device main body structure and is used for protecting an internal structure and reducing energy loss of an internal thermal cycling system; the temperature control instrument is fixedly arranged in the thermal cycle shell and is used for controlling the temperature of the experimental environment; the thermal cycle shell cover is rotationally connected to the thermal cycle shell, and a layer of heat insulation pad is additionally arranged in the thermal cycle shell and on the thermal cycle shell cover, so that the outward diffusion of internal heat is slowed down, and the energy loss is reduced; the thermal fluid circulation assembly is fixedly arranged in the thermal circulation shell and internally holds heat transfer fluid; the device can solve the problems that the existing analyzer is low in circulation efficiency, the sample is heated unevenly and cannot be controlled accurately.
Description
Technical Field
The utility model belongs to the technical field of experimental consumables, and particularly relates to a thermal cycling device of a polymerase chain reaction analyzer.
Background
PCR (polymerase chain reaction) is a common molecular biological technique used to amplify specific DNA fragments. The PCR thermal cycling device is an instrument capable of automatically controlling a PCR reaction system, and can control the processes of denaturation, combination, amplification and the like of DNA in the PCR reaction by circularly changing different temperatures. At present, the PCR thermal cycling device is widely applied in the fields of life science research, medical clinic, forensic science, environmental detection and the like. The PCR technology is widely applied, and can be applied to the fields of rapid identification and separation of diseases and pathogens detection, vaccine production and the like besides the basic research field. Therefore, research and development of the PCR thermal cycler are also in progress, and many different products of the PCR thermal cycler are also on the market. The thermostable enzymes, primers and the like used in the PCR thermal cycling device are also continuously improved, and the stability of the PCR reaction is improved.
The existing thermal cycling devices of the polymerase chain reaction analyzer have the problems that the cycling efficiency is low, the sample is heated unevenly, and the accurate control cannot be realized.
Disclosure of Invention
In view of the above, the utility model provides a thermal cycling device of a polymerase chain reaction analyzer, which can solve the problems that the existing analyzer has low cycling efficiency, and samples are heated unevenly and cannot be controlled accurately.
The utility model is realized in the following way:
the utility model provides a thermal cycling device of a polymerase chain reaction analyzer, which comprises a thermal cycling shell, a temperature control instrument, a thermal cycling shell cover, a sample storage container, a thermal fluid cycling assembly and a controller, wherein the thermal cycling shell is of a device main body structure and is used for protecting an internal structure and reducing energy loss of an internal thermal cycling system; the temperature control instrument is fixedly arranged in the thermal cycle shell and is used for controlling the temperature of an experimental environment; the thermal cycle shell cover is rotationally connected to the thermal cycle shell, and a layer of heat insulation pad is additionally arranged in the thermal cycle shell and on the thermal cycle shell cover and used for slowing down outward diffusion of internal heat and reducing energy loss; the thermal fluid circulation assembly is fixedly arranged in the thermal circulation shell, and internally holds heat transfer fluid for transferring temperature and buffering temperature; the sample storage container is mounted within the thermal fluid circulation assembly and is capable of being disassembled and assembled.
The thermal cycling device of the polymerase chain reaction analyzer has the following technical effects: by arranging the thermal cycle shell, the thermal cycle shell is placed on an analyzer, a space required by reaction is provided, an installation area is reserved for a device for providing reaction conditions, and a certain protection effect is achieved; temperature control is realized by arranging a temperature control instrument, so that the environmental conditions required by the reaction are ensured; providing a reaction site by providing a sample storage container while ensuring that conditions required for the reaction can be transferred to the inside; by arranging the thermal fluid circulation assembly, thermal circulation is realized, and the temperature control efficiency is improved.
Based on the technical scheme, the thermal cycling device of the polymerase chain reaction analyzer can be improved as follows:
the temperature control instrument comprises a heating component and a cooling component, wherein the heating component and the cooling component are fixed on two sides of the bottom of the thermal cycle shell; the temperature rising component is used for rapidly rising the temperature of the heat transfer fluid in the hot fluid circulation component; the temperature reduction assembly is used for rapidly reducing the temperature of the heat transfer fluid in the hot fluid circulation assembly.
Further, the heating component comprises a semiconductor laser, a beam focusing lens, an optical fiber and a heating component bracket, wherein the heating component bracket is fixedly connected to the bottom of the thermal cycle shell and is used for supporting the upper heating device; the semiconductor laser is fixedly connected to the temperature rising component bracket and used for emitting laser to heat the optical fiber; the beam focusing lens is fixedly connected to the semiconductor laser and is used for converging laser emitted by the semiconductor laser to improve heating efficiency; the optical fiber is fixed on the heating assembly bracket and is simultaneously abutted with the bottom of the hot fluid circulation assembly.
The beneficial effects of adopting above-mentioned improvement scheme are: the device is fixed in the thermal cycle shell through arranging the heating assembly bracket, so that the mutual influence of internal components is prevented; by arranging a semiconductor laser, emitting laser to heat the optical fiber; by arranging the beam focusing lens, the beam focusing lens is covered on the semiconductor laser, and laser emitted by the semiconductor laser is gathered, so that the heating efficiency is improved, and the heating time is reduced; by providing an optical fiber, a carrier with a temperature change is provided to achieve an increase in the temperature of the heat transfer fluid in the circulation conduit.
Further, the cooling component comprises a gas compressor, a gas refrigerator, a cooling component bracket and a cooling box, wherein the cooling component bracket is fixedly arranged at the bottom of the thermal cycle shell; the gas compressor is fixedly connected to the cooling component bracket, is communicated with the used gas and is used for sucking the gas and compressing the gas; the gas refrigerator is fixedly connected to the cooling component bracket and communicated with the gas refrigerator through a pipeline, and is used for cooling the gas compressed by the gas compressor; the cooling box is fixedly arranged on the circulating pipeline; for reducing the temperature of the heat transfer fluid in the circulation conduit.
The beneficial effects of adopting above-mentioned improvement scheme are: the device is fixed in the thermal cycle shell through the cooling component bracket, so that the internal components are prevented from being affected mutually; by arranging the gas compressor, the external gas is compressed, and is changed into liquid, so that the rapid cooling is facilitated; by providing a gas refrigerator, the temperature of the liquid compressed by the gas compressor is reduced; by arranging the cooling box, the temperature of the heat transfer fluid in the circulating pipeline is reduced.
Further, the hot fluid circulation assembly comprises a circulation pipeline and a circulation pump, and the circulation pipeline is fixedly arranged in the thermal circulation shell; the circulating pump is fixedly arranged on the circulating pipeline and used for controlling the flow speed of the heat transfer fluid in the circulating pipeline.
Further, the device also comprises a controller, wherein the controller is electrically connected with the semiconductor laser, the gas compressor, the gas refrigerator and the circulating pump.
The beneficial effects of adopting above-mentioned improvement scheme are: by providing a controller, the internal device is controlled to maintain the desired environmental conditions for the reaction.
Further, two pipeline passages are communicated between the cooling box and the gas refrigerator and are used for flowing out and flowing in liquid cooled by the cooling component bracket.
Further, the cooling box structure is a square shell with a central hole inside.
The beneficial effects of adopting above-mentioned improvement scheme are: by arranging the cooling box, the temperature of the heat transfer fluid in the circulating pipeline is fully reduced by utilizing the circulating rolling of the internal liquid.
Furthermore, the circulating pipeline is made of copper materials and is in an annular cylindrical shape, so that the highest heat transfer effect is ensured.
The circulating pipeline is made of copper materials, so that the heat transfer efficiency is greatly improved.
Further, the sample storage container is made of an aluminum material and is flat and long.
The sample storage container is made of aluminum materials, and is used for transferring temperature and avoiding affecting internal reaction.
Compared with the prior art, the thermal cycling device of the polymerase chain reaction analyzer has the beneficial effects that: by arranging the thermal cycle shell, the thermal cycle shell is placed on an analyzer, a space required by reaction is provided, an installation area is reserved for a device for providing reaction conditions, and a certain protection effect is achieved; temperature control is realized by arranging a temperature control instrument, so that the environmental conditions required by the reaction are ensured; providing a reaction site by providing a sample storage container while ensuring that conditions required for the reaction can be transferred to the inside; by arranging the thermal fluid circulation assembly, thermal circulation is realized, and the temperature control efficiency is improved.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present utility model, the drawings that are needed in the description of the embodiments of the present utility model will be briefly described below, it being obvious that the drawings in the following description are only some embodiments of the present utility model, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram showing the overall structure of a thermal cycling apparatus of a polymerase chain reaction analyzer;
FIG. 2 is a schematic diagram of a heating assembly of a thermal cycling apparatus of a polymerase chain reaction analyzer;
FIG. 3 is a schematic diagram of a cooling assembly of a thermal cycling apparatus of a polymerase chain reaction analyzer;
FIG. 4 is an electrical connection diagram of a thermal cycling apparatus of a polymerase chain reaction analyzer;
in the drawings, the list of components represented by the various numbers is as follows:
10. a thermal cycle housing; 20. a temperature control instrument; 21. a temperature raising component; 211. a semiconductor laser; 212. a beam focusing lens; 213. an optical fiber; 214. a temperature raising component bracket; 22. a cooling component; 221. a gas compressor; 222. a gas refrigerator; 223. a cooling component bracket; 224. a cooling box; 30. a thermal cycle housing cover; 40. a sample storage container; 50. a thermal fluid circulation assembly; 51. a circulation pipe; 52. a circulation pump; 60. and a controller.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present utility model more apparent, the technical solutions of the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model, and it is apparent that the described embodiments are some embodiments of the present utility model, but not all embodiments. All other embodiments, based on the embodiments of the utility model, which are apparent to those of ordinary skill in the art without inventive faculty, are intended to be within the scope of the utility model.
Thus, the following detailed description of the embodiments of the utility model, as presented in the figures, is not intended to limit the scope of the utility model, as claimed, but is merely representative of selected embodiments of the utility model. All other embodiments, based on the embodiments of the utility model, which are apparent to those of ordinary skill in the art without inventive faculty, are intended to be within the scope of the utility model.
It should be noted that: like reference numerals and letters denote like items in the following figures, and thus once an item is defined in one figure, no further definition or explanation thereof is necessary in the following figures.
In the description of the present utility model, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings are merely for convenience in describing the present utility model and simplifying the description, and do not indicate or imply that the apparatus or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present utility model.
Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more such feature. In the description of the present utility model, the meaning of "a plurality" is two or more, unless explicitly defined otherwise.
Referring to fig. 1 to 4, in a first embodiment of a thermal cycling apparatus for a pcr analyzer according to the present utility model, the thermal cycling apparatus includes a thermal cycling housing 10, a temperature control device 20, a thermal cycling housing cover 30, a sample storage container 40, a thermal fluid cycling assembly 50 and a controller 60, wherein the thermal cycling housing 10 is a main structure of the apparatus for protecting an internal structure and reducing energy loss of the internal thermal cycling system; the temperature control instrument 20 is fixedly arranged in the thermal cycle shell 10 and is used for controlling the temperature of an experimental environment; the thermal cycle shell cover 30 is rotationally connected to the thermal cycle shell 10, and a layer of heat insulation pad is additionally arranged in the thermal cycle shell 10 and on the thermal cycle shell cover 30, so that outward diffusion of internal heat is slowed down, and energy loss is reduced; the thermal fluid circulation assembly 50 is fixedly installed in the thermal circulation housing 10, and internally holds a heat transfer fluid for transferring temperature and buffering temperature; the sample storage container 40 is mounted within the thermal fluid circulation assembly 50 to enable removal and installation.
In the above technical solution, the temperature control apparatus 20 includes a temperature raising component 21 and a temperature lowering component 22, where the temperature raising component 21 and the temperature lowering component 22 are fixed on two sides of the bottom of the thermal cycle housing 10; the temperature raising assembly 21 is used to rapidly raise the temperature of the heat transfer fluid in the thermal fluid circulation assembly 50; the cooling module 22 is used to rapidly reduce the temperature of the heat transfer fluid in the thermal fluid circulation module 50.
Further, in the above technical solution, the temperature raising component 21 includes a semiconductor laser 211, a beam focusing lens 212, an optical fiber 213, and a temperature raising component support 214, where the temperature raising component support 214 is fixedly connected to the bottom of the thermal cycle housing 10, for supporting the upper heating device; the semiconductor laser 211 is fixedly connected to the temperature raising component bracket 214 and is used for emitting laser light to heat the optical fiber 213; the beam focusing lens 212 is fixedly connected to the semiconductor laser 211 and is used for converging laser emitted by the semiconductor laser 211 to improve heating efficiency; the optical fiber 213 is fixed to the temperature raising member support 214 while abutting against the bottom of the thermal fluid circulation assembly 50.
When the laser device is used, the semiconductor laser 211 emits laser, the laser is converged under the action of the beam focusing lens 212, the laser heating effect is improved, the converged laser is irradiated onto the optical fiber 213, and the optical fiber 213 is rapidly heated under the irradiation of the laser, so that the temperature change is realized.
Further, in the above technical solution, the cooling component 22 includes a gas compressor 221, a gas refrigerator 222, a cooling component bracket 223, and a cooling tank 224, where the cooling component bracket 223 is fixedly installed at the bottom of the thermal cycle housing 10; the gas compressor 221 is fixedly connected to the cooling component bracket 223, and is communicated with the used gas, and is used for sucking the gas and compressing the gas; the gas refrigerator 222 is fixedly connected to the cooling component bracket 223 and is communicated with the gas refrigerator 222 through a pipeline for cooling the gas compressed by the gas compressor 221; the cooling box 224 is fixedly installed on the circulation pipeline 51; for reducing the temperature of the heat transfer fluid in the circulation conduit 51.
In use, the gas compressor 221 compresses gas to a liquid state, the temperature is reduced, and the liquid formed after compression is delivered to the gas refrigerator 222; the gas refrigerator 222 cools the liquid conveyed by the gas compressor 221, and conveys the cooled liquid into the cooling tank 224, the cooling tank 224 receives the low-temperature liquid conveyed by the gas refrigerator 222, the cooling of the circulating pipeline 51 is performed through the rolling of the internal liquid, the cooled liquid returns to the gas refrigerator 222 again through the cooling tank 224 for cooling, and the cooled liquid is conveyed back to the cooling tank 224 after the temperature is reduced, and is reciprocally circulated.
Further, in the above technical solution, the hot fluid circulation assembly 50 includes a circulation pipe 51 and a circulation pump 52, and the circulation pipe 51 is fixedly installed in the thermal circulation housing 10; a circulation pump 52 is fixedly installed on the circulation pipe 51 for controlling a flow rate of the heat transfer fluid inside the circulation pipe 51.
Further, in the above technical solution, the apparatus further includes a controller 60, and the controller 60 is electrically connected to the semiconductor laser 211, the gas compressor 221, the gas refrigerator 222, and the circulation pump 52.
When the circulating pump 52 is used, the power supply is switched on to start working, the controller 60 sends out a control signal, the semiconductor laser 211 receives the control signal to start working, the laser is sent out to heat the optical fiber 213, the temperature of the optical fiber 213 is increased, the temperature is transmitted to the sample storage container 40 through the circulating pipeline 51, and the temperature of the sample storage container 40 is increased; the controller 60 sends out a control signal, the semiconductor laser 211, the gas compressor 221 and the gas refrigerator 222 receive the signal, the semiconductor laser 211 is stopped, the gas compressor 221 starts to operate, absorbs gas and compresses the gas, and then the compressed liquid gas is conveyed to the gas refrigerator 222; the gas refrigerator 222 operates to cool the liquid gas supplied from the gas compressor 221, and to supply the cooled liquid gas to the cooling tank 224. The cooling box 224 transfers the temperature to the circulation pipe 51, and the circulation pipe 51 transfers the temperature to the sample storage container 40 through the internal heat transfer fluid, and the temperature of the sample storage container 40 is lowered. This workflow is then looped.
Further, in the above technical solution, two pipe passages are communicated between the cooling tank 224 and the gas refrigerator 222, and are used for flowing out and flowing in the liquid cooled by the cooling component support 223.
Further, in the above technical solution, the cooling box 224 is a square housing with a central hole therein.
Further, in the above technical solution, the circulation pipe 51 is made of copper material, and is in a circular cylindrical shape, so as to ensure the highest heat transfer effect.
The circulation pipe 51 is made of copper material, so that the heat transfer efficiency is greatly improved.
Further, in the above-described embodiments, the sample storage container 40 is made of an aluminum material and has a flat elongated shape.
The sample storage container 40 is made of an aluminum material, and transfers temperature while avoiding affecting internal reactions.
Specifically, the principle of the utility model is as follows: the circulating pump 52 is powered on to start working, the controller 60 sends out a control signal, the semiconductor laser 211 receives the control signal to start working, the laser is sent out to heat the optical fiber 213, the temperature of the optical fiber 213 is increased to be transmitted to the sample storage container 40 through the circulating pipeline 51, and the temperature of the sample storage container 40 is increased; the controller 60 sends out a control signal, the semiconductor laser 211, the gas compressor 221 and the gas refrigerator 222 receive the signal, the semiconductor laser 211 is stopped, the gas compressor 221 starts to operate, absorbs gas and compresses the gas, and then the compressed liquid gas is conveyed to the gas refrigerator 222; the gas refrigerator 222 operates to cool the liquid gas supplied from the gas compressor 221, and to supply the cooled liquid gas to the cooling tank 224. The cooling box 224 transfers the temperature to the circulation pipe 51, and the circulation pipe 51 transfers the temperature to the sample storage container 40 through the internal heat transfer fluid, and the temperature of the sample storage container 40 is lowered. This workflow is then looped.
The foregoing is merely illustrative of the present utility model, and the present utility model is not limited thereto, and any person skilled in the art will readily recognize that variations or substitutions are within the scope of the present utility model. Therefore, the protection scope of the utility model is subject to the protection scope of the claims.
Claims (10)
1. The thermal cycling device of the polymerase chain reaction analyzer is characterized by comprising a thermal cycling shell (10), a temperature control instrument (20), a thermal cycling shell cover (30), a sample storage container (40), a thermal fluid cycling assembly (50) and a controller (60), wherein the thermal cycling shell (10) is of a device main body structure and is used for protecting an internal structure and reducing energy loss of an internal thermal cycling system; the temperature control instrument (20) is fixedly arranged in the thermal cycle shell (10); the thermal cycle shell cover (30) is rotationally connected to the thermal cycle shell (10), and a layer of heat insulation pad is additionally arranged in the thermal cycle shell (10) and on the thermal cycle shell cover (30) and used for slowing down outward diffusion of internal heat and reducing energy loss; the thermal fluid circulation assembly (50) is fixedly arranged in the thermal circulation shell (10), and internally holds heat transfer fluid for transferring temperature and buffering temperature; the sample storage container (40) is mounted within the thermal fluid circulation assembly (50).
2. The thermal cycling device of the polymerase chain reaction analyzer according to claim 1, wherein the temperature control instrument (20) comprises a heating component (21) and a cooling component (22), and the heating component (21) and the cooling component (22) are fixed at two sides of the bottom of the thermal cycling housing (10); -said temperature increasing assembly (21) for rapidly increasing the temperature of the heat transfer fluid in said hot fluid circulation assembly (50); the cooling assembly (22) is configured to rapidly reduce the temperature of the heat transfer fluid in the hot fluid circulation assembly (50).
3. The thermal cycling device of the polymerase chain reaction analyzer as defined in claim 2, wherein the temperature raising component (21) comprises a semiconductor laser (211), a beam focusing lens (212), an optical fiber (213) and a temperature raising component bracket (214), wherein the temperature raising component bracket (214) is fixedly connected to the bottom of the thermal cycling housing (10) and is used for supporting the upper heating device; the semiconductor laser (211) is fixedly connected to the temperature-raising component bracket (214) and is used for emitting laser to heat the optical fiber (213); the beam focusing lens (212) is fixedly connected to the semiconductor laser (211) and is used for converging laser emitted by the semiconductor laser (211) to improve heating efficiency; the optical fiber (213) is fixed on the temperature rising assembly bracket (214) and is abutted with the bottom of the hot fluid circulation assembly (50).
4. A thermal cycling device of a polymerase chain reaction analyzer according to claim 3, characterized in that the cooling component (22) comprises a gas compressor (221), a gas refrigerator (222), a cooling component support (223) and a cooling box (224), the cooling component support (223) is fixedly installed at the bottom of the thermal cycling housing (10); the gas compressor (221) is fixedly connected to the cooling component bracket (223) and communicated with the used gas, and is used for sucking the gas and compressing the gas; the gas refrigerator (222) is fixedly connected to the cooling component bracket (223) and is communicated with the gas refrigerator (222) through a pipeline, and is used for cooling the gas compressed by the gas compressor (221); the cooling box (224) is fixedly arranged on the circulating pipeline (51); for reducing the temperature of the heat transfer fluid in the circulation conduit (51).
5. The thermal cycling device of a polymerase chain reaction analyzer as defined in claim 4, wherein the thermal fluid cycling assembly (50) comprises the cycling pipe (51) and a cycling pump (52), the cycling pipe (51) being fixedly installed in the thermal cycling housing (10); the circulating pump (52) is fixedly installed on the circulating pipe (51) for controlling the flow rate of the heat transfer fluid inside the circulating pipe (51).
6. The thermal cycling device of the polymerase chain reaction analyzer of claim 5, further comprising a controller (60), wherein the controller (60) is electrically connected to the semiconductor laser (211), the gas compressor (221), the gas refrigerator (222) and the circulation pump (52).
7. The thermal cycling device of the polymerase chain reaction analyzer according to claim 6, wherein two pipeline paths are communicated between the cooling box (224) and the gas refrigerator (222) for flowing out and flowing in the liquid cooled by the cooling component bracket (223).
8. The thermal cycling device of the polymerase chain reaction analyzer of claim 7, wherein the cooling box (224) is a square housing with a central hole inside.
9. The thermal cycling device of polymerase chain reaction analyzer as set forth in claim 8, wherein the cycling tube (51) is made of copper material and is ring-shaped and cylindrical for ensuring heat transfer effect.
10. The thermal cycler of the polymerase chain reaction analyzer of claim 9, wherein the sample holder (40) is made of an aluminum material and is formed in a flat elongated shape.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320909519.6U CN219861309U (en) | 2023-04-21 | 2023-04-21 | Thermal cycling device of polymerase chain reaction analyzer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320909519.6U CN219861309U (en) | 2023-04-21 | 2023-04-21 | Thermal cycling device of polymerase chain reaction analyzer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219861309U true CN219861309U (en) | 2023-10-20 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202320909519.6U Active CN219861309U (en) | 2023-04-21 | 2023-04-21 | Thermal cycling device of polymerase chain reaction analyzer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219861309U (en) |
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2023
- 2023-04-21 CN CN202320909519.6U patent/CN219861309U/en active Active
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