CN210314197U - PCR amplification detection device - Google Patents
PCR amplification detection device Download PDFInfo
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- CN210314197U CN210314197U CN201920764897.3U CN201920764897U CN210314197U CN 210314197 U CN210314197 U CN 210314197U CN 201920764897 U CN201920764897 U CN 201920764897U CN 210314197 U CN210314197 U CN 210314197U
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Abstract
The utility model discloses a PCR amplification detection device, which comprises a PCR module for realizing DNA amplification, an optical scanning system for detecting the PCR module and an air cooling system for cooling the PCR module, wherein the PCR module is arranged above the optical scanning system, and the PCR module can move back and forth between the optical scanning system and the air cooling system; an air duct for air to flow vertically is arranged in the air cooling system. The PCR module is arranged to be movable above the optical scanning system, so that photometry can be performed at the bottom of the PCR module, and the defect that photometry is easily interfered due to the fact that water mist is easily generated due to the fact that the PCR module is alternated at high and low temperatures is effectively overcome; meanwhile, the air duct in the air cooling system is set to be the vertical air duct, the phenomenon that water vapor in the air duct easily falls to the heat dissipation mechanism of the PCR module at the bottom to influence the heat dissipation of the PCR module is avoided, and meanwhile, dust is prevented from falling and nucleic acid in the PCR module is prevented from being polluted.
Description
Technical Field
The utility model belongs to the technical field of molecular diagnosis, concretely relates to PCR amplifys detection device.
Background
In the fields of molecular biology and in vitro diagnostics, both for research needs and clinical diagnostics, it is necessary to detect specific biomolecules in a sample quantitatively or qualitatively, such as: specific nucleic acid sequence or a certain protein to perform biological information inversion and disease diagnosis. Colloquially, all molecular biology-based methodology techniques are well known as molecular diagnostic techniques, such as PCR techniques, gene sequencing techniques, and the like.
The PCR technique, polymerase chain reaction, is a molecular biology technique for amplifying specific DNA fragments, the basic principle is similar to the natural DNA replication process, and the PCR technique comprises three basic reaction steps of denaturation, annealing and extension: denaturation of the template DNA, annealing (renaturation) of the template DNA to the primer, and extension of the primer. Repeating the three processes of denaturation, annealing and extension to obtain more semi-reserved copy chains, wherein the new chains can become templates of the next cycle.
In most detection equipment in the market at the existing stage, a PCR module is fixedly arranged, an optical scanning detection system can move, light is measured from the top, but water mist is easily generated due to the alternation of high temperature and low temperature of the PCR module, so that the defect that the light measurement is easily interfered during the light measurement is overcome; in addition, the air duct in the existing detection equipment is horizontally arranged, and water vapor in the air duct easily falls to the PCR module heat dissipation mechanism at the bottom to influence the heat dissipation of the PCR module heat dissipation mechanism.
Disclosure of Invention
The utility model aims at providing a PCR amplifications detection device, solve current detection device on the one hand because the wind channel level sets up, on the other hand because the PCR module makes the problem that easily receives the interference when surveying optics because of the water smoke that high low temperature produced in turn.
In order to solve the technical problem, the utility model discloses a following technical scheme realizes:
a PCR amplification detection device comprises a PCR module for realizing DNA amplification, an optical scanning system for detecting the PCR module and an air cooling system for cooling the PCR module, wherein the PCR module is arranged above the optical scanning system and can move back and forth between the optical scanning system and the air cooling system; the air cooling system is provided with an air duct for supplying air to vertically flow.
Specifically, the PCR module is connected with a moving mechanism through a base, and a guide rail for guiding the moving mechanism is arranged at the bottom of the moving mechanism.
Specifically, the moving mechanism comprises a motor and a lead screw connected to the motor; the base comprises a first connecting end, a second connecting end and a third connecting end, wherein the first connecting end is connected with the PCR module, a guide groove matched with the guide rail is formed in the bottom of the second connecting end, the third connecting end is in threaded connection with the lead screw, and the whole base and the PCR module are driven to move back and forth above the air cooling system and the optical scanning system through rotation of the lead screw.
Specifically, the base comprises an L-shaped plate body and two end plate bodies of a ribbed plate L-shaped plate body arranged on the L-shaped plate body to form the first connecting end and the second connecting end, and the ribbed plate forms the third connecting end.
Further, this detection device still includes the base, air cooling system, moving mechanism and optical scanning system all set up on the base, wherein, air cooling system hangs in the top of base through a support column, and the guide rail is fixed on the base, and air cooling system and optical scanning system lie in same one side of guide rail.
Specifically, the air cooling system is a vertical cylinder, an air inlet is formed in the bottom of the cylinder, an air outlet is formed in the top of the cylinder, and an exhaust fan is arranged at the air outlet; the PCR module is provided with a heat dissipation mechanism, and the heat dissipation mechanism is arranged above the air outlet.
Specifically, the cylinder is provided with a slot for inserting the heat dissipation mechanism, and the slot penetrates through the cylinder along the horizontal direction.
Compared with the prior art, the beneficial effects of the utility model are that:
(1) the utility model discloses with optical scanning system fixed mounting, set up the PCR module into at optical scanning system top movable, can realize like this that PCR module bottom photometry, the effectual PCR module of having avoided is because of high low temperature is in turn, easily produces water smoke for receive the shortcoming of interference easily when photometry, need not to increase the cotton defogging of heating.
(2) The utility model discloses a set up wind channel in the air cooling system into vertical wind channel, avoid the PCR module heat dissipation mechanism of bottom that steam in the wind channel easily falls, influence its heat dissipation, prevent to fall the ash and pollute the nucleic acid in the PCR module simultaneously.
Other features and advantages of the present invention will be described in detail in the detailed description which follows.
Drawings
Fig. 1 is an axonometric view of the overall structure of the present invention.
Fig. 2 is an axonometric view of the overall structure of the present invention.
The reference numerals in the figures denote:
1-PCR module, 2-optical scanning system, 3-air cooling system, 4-base, 5-moving mechanism, 6-guide rail, 7-base, 8-support column;
11-a heat dissipation mechanism;
31-inlet, 32-outlet, 33-exhaust fan, 34-slot
41-a first connecting end, 42-a second connecting end, 43-a third connecting end and 44-a guide groove;
51-motor, 52-screw rod, 53-fixed disk.
The following detailed description of the present invention is provided in connection with the accompanying drawings and the detailed description of the invention.
Detailed Description
The PCR module 1 in the utility model is the existing PCR module on the market, which comprises a temperature control module and a temperature measuring module, wherein the temperature control module comprises a refrigerating piece and a radiating piece, and the temperature of the whole PCR module is controlled to rise and fall by changing the current direction connected into the refrigerating piece; the temperature measurement module is used for detecting the temperature of the whole PCR module in real time.
The utility model discloses the principle that well PCR detected does:
double-stranded DNA (template) → high temperature denaturation (separation of double strands into single strands) → low temperature annealing (hybridization of template to primer) → extension (DNA synthesis)
The following embodiments of the present invention are given, and it should be noted that the present invention is not limited to the following embodiments, and all the equivalent transformations made on the basis of the technical solution of the present application all fall into the protection scope of the present invention.
In the present invention, unless otherwise specified, the use of directional terms such as "upper" and "lower" generally means that the terms are defined with reference to the drawing plane of the corresponding drawing, and "inner" and "outer" mean that the terms are inner and outer relative to the outline of the corresponding part.
As shown in FIG. 1, the utility model discloses a PCR amplifications detection device, including the PCR module 1 that is used for realizing DNA amplification, the optical scanning system 2 that is used for detecting the PCR module and be used for the air cooling system 3 to the PCR module cooling, wherein, PCR module 1 sets up in optical scanning system 2's top, and PCR module 1 can be at optical scanning system 2 and air cooling system 3 between round trip movement.
The PCR module 1 is connected with a moving mechanism 5 through a base 4, and a guide rail 6 for guiding the moving mechanism 5 is arranged at the bottom of the moving mechanism 4. As a preferred example of the present invention, the moving mechanism 5 includes a motor 51 and a lead screw 52 connected to the motor 51. Base 4 includes first link 41, second link 42 and third link 43, it is concrete, for convenient connection and compact structure nature, base 4 comprises L type plate body and the floor of setting on L type plate body, the both ends plate body of L type plate body forms first link 41 and second link 42, the floor forms third link 43, wherein, first link 41 is connected with the PCR module, for convenient dismantlement, be provided with the screw hole on L type plate body, the PCR module passes through the fix with screw on L type plate body. The bottom of the second connecting end 42 is provided with a guide groove 44 matched with the guide rail 6, and the third connecting end 43 drives the whole base 4 and the PCR module 1 to move back and forth above the air cooling system 3 and the optical scanning system 2 through the rotation of the screw 52.
The stiffening rib is fixed in the centre of L type plate body, and the stiffening rib is positive trapezoidal plate body, and the double-phase adjacent right angle side of positive trapezoidal plate body is fixed respectively on the both ends plate body of L type plate body, and the slope limit of positive trapezoidal plate body is outside.
The ribbed plate is provided with a threaded hole for the lead screw 52 to pass through, two fixed disks 53 are fixed on two sides of the threaded hole, the fixed disks 53 are used for supporting the lead screw 52 to stably move, the two fixed disks 53 are also provided with threaded holes, the ribbed plate is in threaded connection with the lead screw 52, the lead screw 52 is driven to rotate through the forward rotation and the reverse rotation of the motor 51, the base 4 drives the PCR module 1 to move back and forth along the guide rail 6, and the PCR module 1 can move back and forth above the air cooling system 3 and the optical scanning system 2.
In the above mode, optical scanning system 2 fixed mounting sets up PCR module 1 to be at optical scanning system 2 top movable, can realize like this that 1 bottom of PCR module is surveyed the light, and the effectual PCR module of having avoided is because of high low temperature is alternative, easily produces water smoke for receive the shortcoming of interference easily during photometry, need not to increase the cotton defogging of heating.
As shown in fig. 2, in an embodiment of the present invention, the air cooling system 3 is a vertical cylinder, and an air inlet 31 is disposed at the bottom of the cylinder, and the air inlet 31 is preferably in a horn shape. The top of barrel is provided with air outlet 32, and air outlet 32 department is provided with exhaust fan 33, forms the wind channel between the air intake 31 of barrel and the air outlet 32, and this wind channel is vertical wind channel, compares in traditional horizontal wind channel, avoids the PCR module heat dissipation mechanism of bottom that steam in the wind channel easily falls, influences its heat dissipation, prevents to fall the ash simultaneously and pollutes the nucleic acid in the PCR module. The PCR module 1 is provided with a heat dissipation mechanism 11, and the heat dissipation mechanism 11 is disposed above the air outlet 32. The heat dissipated by the heat dissipation mechanism is sent to the outside through the air entering the cylinder, wherein the heat dissipation mechanism is an existing PCR module heat dissipation mechanism on the market.
In addition, a slot 34 for inserting the heat dissipation mechanism 11 is arranged on the barrel, the slot 34 is positioned above the air inlet, and the slot 34 penetrates through the barrel along the horizontal direction, so that the heat dissipation mechanism 11 can move horizontally along with the movement of the PCR module 1. The arrangement of the slot 34 facilitates the insertion of the heat dissipation mechanism 11 into the air duct, and the heat dissipation mechanism has a compact structure and is beneficial to the miniaturization of equipment.
In order to improve the integrity of the device, a base 7 is arranged on the detection device, and the air cooling system 3, the moving mechanism 5 and the optical scanning system 2 are all integrated on the base 7, wherein the air cooling system 3 is suspended above the base 7 through a supporting column 8, the optical scanning system 2 is fixed on the base 7, and the air cooling system 3 and the optical scanning system 2 are arranged on the same side of the guide rail 6. Guide rail 6 also fixes on base 7, and is concrete, evenly sets up a plurality of screw holes on the guide rail 6, fixes on base 7 through the screw, convenient dismantlement.
Based on PCR detection principle, the utility model discloses a testing process does:
firstly, controlling an automatic temperature control system in the PCR module 1 to heat up, and controlling an air cooling system 3 to reduce the air quantity, so that the temperature of an amplification temperature block in the PCR module 1 is raised to 90-95 ℃, and DNA double strands are divided into single strands;
secondly, controlling an automatic temperature control system in the PCR module 1 to cool down, and controlling an air cooling system 3 to increase the air volume, so that the temperature of an amplification temperature block in the PCR module 1 is reduced to 50-65 ℃, a template and a primer are hybridized, and DNA is synthesized;
thirdly, controlling the motor 51 to rotate in a low temperature section (50-65 ℃) to drive the PCR module 1 to move on the guide rail 6 to the multi-channel optical scanning system 2 along with the base 4 to carry out optical scanning detection;
fourthly, after the optical scanning detection is finished, controlling the motor 51 to rotate to drive the PCR module 1 to move to the slot 34 on the air inlet 31 of the air cooling system 3 along with the base 4 on the guide rail 6;
and repeating the steps.
In addition, the utility model discloses a PCR module 1 only when optical scanning detects, just control it and follow base 4 and move on guide rail 6 to multichannel optical scanning system 3, all the other times all in fluting 34.
The various features described in the foregoing detailed description can be combined in any suitable manner without departing from the spirit of the invention, and should also be construed as disclosed in the invention.
Claims (7)
1. A PCR amplification detection device comprises a PCR module (1) for realizing DNA amplification, an optical scanning system (2) for detecting the PCR module and an air cooling system (3) for cooling the PCR module, and is characterized in that,
the PCR module (1) is arranged above the optical scanning system (2), and the PCR module (1) can move back and forth between the optical scanning system (2) and the air cooling system (3); and an air duct for supplying air to vertically flow is arranged in the air cooling system (3).
2. The PCR amplification detecting apparatus according to claim 1, wherein the PCR module (1) is connected to the moving mechanism (5) through a base (4), and a guide rail (6) for guiding the moving mechanism (5) is provided at the bottom of the moving mechanism (5).
3. The apparatus for detecting PCR amplification of claim 2, wherein the moving mechanism (5) comprises a motor (51) and a screw (52) connected to the motor (51); the base (4) comprises a first connecting end (41), a second connecting end (42) and a third connecting end (43), wherein the first connecting end (41) is connected with the PCR module, a guide groove (44) matched with the guide rail (6) is formed in the bottom of the second connecting end (42), the third connecting end (43) is in threaded connection with a lead screw (52), and the whole base (4) and the PCR module (1) are driven to move back and forth above the air cooling system (3) and the optical scanning system (2) through rotation of the lead screw (52).
4. The PCR amplification detecting apparatus according to claim 3, wherein the base (4) comprises an L-shaped plate and a rib plate disposed on the L-shaped plate, wherein the two end plates of the L-shaped plate form the first connecting end (41) and the second connecting end (42), and the rib plate forms the third connecting end (43).
5. The PCR amplification detecting apparatus according to claim 2, further comprising a base (7), wherein the air cooling system (3), the moving mechanism (5) and the optical scanning system (2) are disposed on the base (7), wherein the air cooling system (3) is suspended above the base (7) by a supporting column (8), the guide rail (6) is fixed on the base (7), and the air cooling system (3) and the optical scanning system (2) are located on the same side of the guide rail (6).
6. The PCR amplification detection device of claim 1, wherein the air cooling system (3) is a vertical cylinder, an air inlet (31) is arranged at the bottom of the cylinder, an air outlet (32) is arranged at the top of the cylinder, and an exhaust fan (33) is arranged at the air outlet (32); the PCR module (1) is provided with a heat dissipation mechanism (11), and the heat dissipation mechanism (11) is arranged at the position, close to the upper part, of the air outlet (32).
7. The PCR amplification detecting apparatus according to claim 6, wherein the barrel is provided with a slot (34) into which the heat dissipating mechanism (11) is inserted, and the slot (34) penetrates the barrel in a horizontal direction.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920764897.3U CN210314197U (en) | 2019-05-24 | 2019-05-24 | PCR amplification detection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920764897.3U CN210314197U (en) | 2019-05-24 | 2019-05-24 | PCR amplification detection device |
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| Publication Number | Publication Date |
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| CN210314197U true CN210314197U (en) | 2020-04-14 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201920764897.3U Active CN210314197U (en) | 2019-05-24 | 2019-05-24 | PCR amplification detection device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112779157A (en) * | 2021-01-08 | 2021-05-11 | 鲲鹏基因（北京）科技有限责任公司 | PCR reaction device with sample preparation function |
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2019
- 2019-05-24 CN CN201920764897.3U patent/CN210314197U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112779157A (en) * | 2021-01-08 | 2021-05-11 | 鲲鹏基因（北京）科技有限责任公司 | PCR reaction device with sample preparation function |
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