CN117402731B - Temperature control device for PCR instrument and PCR instrument - Google Patents
Temperature control device for PCR instrument and PCR instrument Download PDFInfo
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- CN117402731B CN117402731B CN202311723619.0A CN202311723619A CN117402731B CN 117402731 B CN117402731 B CN 117402731B CN 202311723619 A CN202311723619 A CN 202311723619A CN 117402731 B CN117402731 B CN 117402731B
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0627—Sensor or part of a sensor is integrated
- B01L2300/0654—Lenses; Optical fibres
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1894—Cooling means; Cryo cooling
Abstract
The invention relates to a temperature control device for a PCR instrument and the PCR instrument, wherein the temperature control device for the PCR instrument comprises: the device comprises a plurality of amplification modules, a plurality of detection modules and a plurality of detection modules, wherein the plurality of amplification modules are configured to respectively place sample tubes for storing samples to be detected, and the plurality of amplification modules are used for respectively carrying out copying amplification treatment on the samples to be detected added into the corresponding sample tubes; the heat dissipation module is connected with the at least one amplification module and is used for assisting the amplification module in heat dissipation and cooling when the amplification module is cooled. The invention can realize the follow-up detection of the sample to be detected, and the detection experiment of the sample to be detected does not need waiting, so that the speed of the output of the detection result of the sample to be detected is increased, and the experimental process of the sample to be detected which is detected at the beginning is not influenced.
Description
Technical Field
The invention relates to the technical field of molecular diagnosis, in particular to a temperature control device for a PCR instrument and the PCR instrument.
Background
The molecular diagnosis technique is a technique for diagnosing a human state and a disease by detecting the presence, defect or abnormal expression of a gene using a molecular biological technique using DNA and RNA as diagnostic materials. The basic principle is to detect whether the structure of DNA or RNA is changed, the quantity of DNA or RNA is more or less and the expression function is abnormal, so as to determine whether the detected person has abnormal change of gene level, and the method has important significance for preventing, predicting, diagnosing, treating and prognosis of diseases. All molecular biology-level-based methodological techniques are colloquially simple, and belong to the group of molecular diagnostic techniques, such as polymerase chain reaction techniques (also known as PCR techniques), gene sequencing techniques, and the like.
The polymerase chain reaction (Polymerase chain reaction, PCR) technique is a molecular biological technique for amplifying a specific DNA fragment (gene to be tested) of a sample to be tested, i.e. a specific in vitro amplification process of the DNA fragment. The basic principle of PCR is as follows: double-stranded DNA is denatured and melted at high temperature to form single-stranded DNA, double strands can be renatured after the temperature is reduced, the denaturation and renaturation of the DNA are controlled through temperature change, and primers, DNA polymerase, deoxyribonucleoside triphosphates (dNTPs) and corresponding buffers are added, so that the in vitro replication and amplification of specific DNA fragments can be completed through temperature control. The basic principle is similar to the natural replication process of DNA, consisting of three basic reaction steps of denaturation-annealing-extension: the three processes of denaturation of template DNA, annealing (renaturation) of the template DNA and a primer, extension of the primer and repeated cycle denaturation, annealing and extension can obtain more half-reserved copy chains, and the new chain can become a template for the next cycle.
The real-time fluorescent quantitative polymerase chain reaction (Quantitative Real-time Polymerase Chain Reaction, qPCR) is to add a reporter group for a specific DNA fragment in a PCR reaction system of a sample to be detected, when the specific DNA fragment undergoes one reaction cycle (namely after undergoing one replication), the fluorescent signal intensity emitted by the reporter group is enhanced once, the real-time monitoring of the change of the reaction product quantity is realized by detecting the change of the fluorescent signal intensity after each reaction cycle, and qualitative and quantitative analysis can be performed on the sample to be detected according to the monitoring result.
At present, a PCR instrument is a key instrument for implementing a PCR technology, and the PCR instrument generally includes a temperature control device and an optical detection device, and the temperature control device of the existing high-throughput PCR instrument generally performs temperature control processing on a plurality of samples to be tested at the same time and performs copy amplification at the same time.
Aiming at the problem that a PCR instrument in the related art cannot realize the detection of a sample to be detected at any time, no effective solution is provided at present.
Therefore, the inventor provides a temperature control device for a PCR instrument and the PCR instrument by virtue of experience and practice of related industries for many years so as to overcome the defects of the prior art.
Disclosure of Invention
The invention aims to provide a temperature control device for a PCR instrument and the PCR instrument, which can realize the follow-up detection of a sample to be detected, the detection experiment of the sample to be detected does not need waiting, so that the speed of the output of the detection result of the sample to be detected is increased, and the experimental process of the sample to be detected which is detected at the beginning is not influenced.
The object of the invention can be achieved by the following scheme:
the invention provides a temperature control device for a PCR instrument, which comprises:
a plurality of amplification modules, wherein the amplification modules are configured to respectively place sample tubes for storing samples to be tested, and the amplification modules are used for respectively carrying out copying and amplification treatment on the samples to be tested, which are added into the corresponding sample tubes;
the heat dissipation module is connected with at least one amplification module and is used for assisting the amplification module in heat dissipation and cooling when the amplification module is cooled.
In a preferred embodiment of the present invention, the heat dissipation module includes a plurality of heat dissipation blocks, and the plurality of heat dissipation blocks are connected to the plurality of amplification modules in a one-to-one correspondence manner, so as to dissipate heat and cool the corresponding amplification modules through the plurality of heat dissipation blocks.
In a preferred embodiment of the present invention, the heat dissipation block includes a plurality of heat dissipation fins and an air deflector arranged side by side, and the air deflector is connected to the heat dissipation fins so as to form an air outlet between the two connected heat dissipation fins on the opposite side of the air deflector.
In a preferred embodiment of the present invention, the plurality of amplification modules are arranged in a plurality of rows in a horizontal direction, and the air deflectors corresponding to the amplification modules in each row have the same arrangement direction, so that the air outlets of the plurality of heat dissipation blocks in each row are led out in the same direction;
the air deflectors corresponding to the amplification modules in each row have different setting directions, so that the air outlets of the plurality of heat dissipation blocks in two adjacent rows respectively emit air in opposite directions.
In a preferred embodiment of the present invention, the heat dissipating block further includes a first air guiding pipe, and the first air guiding pipe is connected to the heat dissipating fin, so as to form an air inlet at the first air guiding pipe.
In a preferred embodiment of the present invention, the heat dissipation module includes an air duct for providing cooling air for a plurality of heat dissipation blocks, the air duct has an air duct and a plurality of air passing holes communicated with the air duct, and the plurality of heat dissipation blocks are respectively connected with the corresponding air passing holes.
In a preferred embodiment of the present invention, the heat dissipation module further includes a plurality of second air guiding pipes, and the plurality of second air guiding pipes are respectively disposed at the corresponding air passing holes;
the second induced air pipe comprises an air inlet section and an air outlet section which are communicated, the air inlet section is positioned in the air duct, and the air outlet section penetrates through the corresponding air passing hole and stretches into the corresponding first induced air pipe.
In a preferred embodiment of the invention, along the length direction of the air pipe, one end of the air pipe is sealed, the other end of the air pipe is provided with an inlet communicated with the air duct, and a fan is arranged at the inlet;
at least part of the air passing holes are distributed on the air pipe at intervals along the length direction of the air pipe.
In a preferred embodiment of the present invention, the amplification module includes a positioning member for providing a placement position for the sample tube, and a heating and cooling module for heating or cooling the sample tube according to a replication and amplification stage of the sample to be tested, where the positioning member has a receiving cavity, the sample tube is located in the receiving cavity, the heating and cooling module is connected to the positioning member, and the heating and cooling module is connected to the heat dissipation module through the positioning member.
In a preferred embodiment of the present invention, the amplification module further includes a heat transfer tube and a heat conductive connection piece, the heat transfer tube includes a tube body and a connection portion disposed at the bottom of the tube body and having a plate shape;
the heating and refrigerating module, the heat conduction connecting sheet and the connecting part are sequentially connected from bottom to top, the tube body penetrates through the accommodating cavity, and the sample tube can enter the accommodating cavity from the top opening of the accommodating cavity and is placed in the tube body.
In a preferred embodiment of the present invention, the amplification module further includes an optical fiber for transmitting excitation light and emission light, a first end of the optical fiber sequentially passes through the positioning element and the tube body and extends to a position where the sample tube is located, and a second end of the optical fiber is used for externally connecting an optical detection device, and the optical detection device is used for emitting excitation light and receiving emission light generated by the sample to be tested.
In a preferred embodiment of the present invention, the positioning member is provided with a first hole communicated with the accommodating cavity, the tube body is provided with a second hole communicated with the interior of the tube body, when the tube body is arranged in the accommodating cavity in a penetrating manner, the first hole is communicated with the second hole, and the first end of the optical fiber sequentially penetrates through the first hole and the second hole to extend into the tube body;
and the positioning piece is provided with a clamp spring which is used for locking or releasing the connection position of the first end of the optical fiber.
In a preferred embodiment of the present invention, the positioning member is provided with a third hole communicated with the first hole, the fixed end of the clamping spring is connected to the positioning member, and the action end of the clamping spring enters the third hole from one side of the third hole and extends out from the other side of the third hole after passing through the communication position between the third hole and the first hole;
and a concave part is arranged on the optical fiber and close to at least part of the first end, and the position of the action end of the clamp spring is adjusted in the third hole so as to drive at least part of the clamp spring in the third hole to be clamped into the concave part or moved out of the concave part.
In a preferred embodiment of the present invention, the temperature control device for PCR instrument further includes a plurality of light shielding covers, and the plurality of light shielding covers are respectively disposed above the corresponding amplification modules.
In a preferred embodiment of the present invention, each of the amplification modules is arranged in a plurality of rows in the horizontal direction;
the heat dissipation module comprises a plurality of air channels, two adjacent air channels are separated through a partition board, and each row of amplification modules are respectively located above the corresponding air channels.
In a preferred embodiment of the present invention, the heat dissipation module is an integral heat dissipation structure formed by arranging a plurality of heat dissipation fins side by side, one end of each heat dissipation fin is provided with a fan, and a plurality of the expansion modules are located at the top of the heat dissipation module.
The invention provides a PCR instrument, which comprises the temperature control device for the PCR instrument.
From the above, the temperature control device for the PCR instrument and the PCR instrument have the characteristics and advantages that: the sample tube can be arranged in each independent amplification module, each sample tube can be used for independently storing samples to be detected, the heat dissipation module connected with the plurality of amplification modules is matched, the heat dissipation and the temperature reduction of the amplification modules can be assisted when the amplification modules are cooled, and the samples to be detected added into the corresponding sample tubes can be subjected to independent and rapid replication and amplification treatment, so that the samples to be detected can be detected and detected in follow-up detection, even if the number of the samples to be detected is huge and the samples cannot be detected at the same time, the samples to be detected which are sent in real time are not required to be waited, the speed of the output of the detection results of the samples to be detected is greatly accelerated, meanwhile, the experimental process of the samples to be detected which are detected is started first is not influenced, and more efficient and instant PCR detection is realized.
Drawings
The following drawings are only for purposes of illustration and explanation of the present invention and are not intended to limit the scope of the invention. Wherein:
fig. 1: one of the structural schematic diagrams of the temperature control device for the PCR instrument is provided;
fig. 2: a partial cross-sectional view of the temperature control device for the PCR instrument;
fig. 3: the connection position of the temperature control device amplification module and the heat dissipation module for the PCR instrument is schematically shown;
fig. 4: an exploded view of a temperature control device amplification module and a heat dissipation module for the PCR instrument;
fig. 5: one of the structural schematic diagrams of the radiating block in the temperature control device for the PCR instrument is provided;
fig. 6: the second schematic diagram is a structure diagram of a heat dissipation block in the temperature control device for the PCR instrument;
fig. 7: the structure schematic diagram of the air pipe in the temperature control device for the PCR instrument is shown;
fig. 8: the structure diagram of the second air guiding pipe in the temperature control device for the PCR instrument is shown;
fig. 9: the optical fiber connection schematic diagram of the positioning piece in the temperature control device for the PCR instrument is provided;
fig. 10: an exploded view of a positioning piece and an optical fiber in the temperature control device for the PCR instrument;
fig. 11: the second schematic diagram is the structure diagram of the temperature control device for the PCR instrument;
fig. 12: the third schematic diagram is the structure diagram of the temperature control device for the PCR instrument.
The reference numerals of the invention are:
1. an amplification module;
101. a positioning piece;
1011. a receiving channel;
1012. a first hole;
1013. a third hole;
1014. a bump;
102. a heat transfer tube;
1021. a tube body;
1022. a connection part;
1023. a second hole;
103. a heat conductive connecting sheet;
104. a heating and refrigerating module;
105. an optical fiber;
1051. a concave portion;
106. clamping springs;
1061. a fixed end;
1062. an action end;
2. a heat dissipation module;
201. a heat dissipation block;
2011. a heat sink;
2012. an air deflector;
2013. a first air guiding pipe;
202. an air duct;
2021. an air duct;
2022. a fan;
2023. a second air guiding pipe;
20231. an air inlet section;
20232. an air outlet section;
2024. an inlet;
3. a sample tube;
4. a light shielding cover;
5. a partition board.
Detailed Description
For a clearer understanding of technical features, objects, and effects of the present invention, a specific embodiment of the present invention will be described with reference to the accompanying drawings.
Embodiment one
As shown in fig. 1 to 12, the present invention provides a temperature control device for a PCR instrument, which includes a plurality of amplification modules 1 and at least one heat dissipation module 2, the plurality of amplification modules 1 being configured to respectively house sample tubes 3 for storing samples to be tested, the plurality of amplification modules 1 being configured to respectively perform a copy amplification process on the samples to be tested added to their corresponding sample tubes 3; the heat dissipation module 2 is connected with at least one amplification module 1, and the heat dissipation module 2 is used for assisting in heat dissipation and cooling of the amplification module 1 when the amplification module 1 is cooled, so that heat dissipation and cooling capacity of the amplification module 1 is improved when the amplification module 1 needs to be cooled, and detection efficiency is improved.
The invention has a plurality of independent amplification modules 1, each independent amplification module 1 can be internally provided with a sample tube 3 respectively, each sample tube 3 can be used for independently storing samples to be detected, and is matched with a heat dissipation module 2 connected with at least one amplification module 1 (preferably, the heat dissipation module 2 is simultaneously connected with a plurality of amplification modules 1), so that the heat dissipation and the cooling of the amplification modules 1 can be assisted when the amplification modules 1 are cooled, and the samples to be detected added into the corresponding sample tubes 3 can be independently and rapidly subjected to replication and amplification treatment, thereby realizing the follow-up detection of the samples to be detected, even if the number of the samples to be detected is huge and the samples to be detected cannot be detected at the same time, the samples to be detected are not required to be waited, the output speed of the detection results of the samples to be detected is greatly accelerated, and the experimental progress of the samples to be detected which are detected firstly cannot be influenced, so that more efficient and instant PCR detection is realized. For the condition that the test time of the sample to be tested of different patients in a hospital is different, the sample to be tested of different test time can be tested at the same time, so that the speed of the output of the test result of the sample to be tested is greatly increased, and the aim of facilitating the timely pathological analysis of the patient is fulfilled.
Specifically, as shown in FIGS. 1 and 2, 10 amplification modules 1 are taken as an example in the present invention, and in the horizontal direction, 10 amplification modules 1 are equally divided into two rows arranged side by side, and 5 amplification modules 1 are arranged in each row. Of course, fig. 1 and 2 are only examples, and other numbers and arrangements of the plurality of amplification modules 1 may be provided during actual use. Because the independent amplification modules 1 are arranged in the PCR detection device, when samples to be detected are sent to be detected at different times, the amplification can be started to be duplicated and amplified in the idle amplification module 1 (namely, the amplification module 1 of the sample tube 3 for storing the samples to be detected is not placed), the experimental process of other samples to be detected, which are subjected to detection experiments, is not influenced, and the follow-up detection with high flux is realized, so that the PCR detection device has the capability of high flux and follow-up detection.
In an alternative embodiment of the present invention, as shown in fig. 1 to 4, the heat dissipation module 2 includes a plurality of heat dissipation blocks 201, the number of the heat dissipation blocks 201 is the same as that of the amplification modules 1, and the plurality of heat dissipation blocks 201 are respectively located below the corresponding amplification modules 1, the plurality of heat dissipation blocks 201 are in one-to-one correspondence with the plurality of amplification modules 1, and the top of the plurality of heat dissipation blocks 201 is connected with the bottom of the corresponding amplification modules 1, so that heat dissipation and cooling can be performed on the corresponding amplification modules 1 through the plurality of heat dissipation blocks 201 respectively.
Further, as shown in fig. 5 and 6, the heat dissipation block 201 includes a plurality of heat dissipation fins 2011 and an air deflector 2012, the plurality of heat dissipation fins 2011 arranged along the vertical direction are arranged side by side to form a rectangular block shape, a heat dissipation gap is left between two adjacent heat dissipation fins 2011, the air deflector 2012 is arranged on one side of the plurality of heat dissipation fins 2011 along the vertical direction, and the air deflector 2012 is connected with the edges of the plurality of heat dissipation fins 2011, so that one side of the heat dissipation gap formed by the plurality of heat dissipation fins 2011 is blocked by the air deflector 2012, and an air outlet is formed between two adjacent heat dissipation fins 2011 on the side opposite to the air deflector 2012. The air deflector 2012 is not only used for connecting the plurality of radiating fins 2011, but also can limit the flow direction of the radiating air flow through the setting position and the direction of the air deflector 2012, so as to ensure good radiating effect and avoid the condition of poor radiating effect caused by guiding the radiating air flow to the same direction or one place.
As shown in fig. 1 and 2, in one embodiment of the present invention, the amplification modules 1 are horizontally arranged in a plurality of rows (two rows in this embodiment), and the air deflectors 2012 corresponding to the amplification modules 1 in each row have the same direction, so that the air outlets of the plurality of heat dissipation blocks 201 in each row discharge air in the same direction, and the heat dissipation air flows discharged by the plurality of heat dissipation blocks 201 in each row keep approximately parallel flow directions, so that no interference is generated between the heat dissipation air flows; the air deflectors 2012 corresponding to the amplification modules 1 in each row have different setting directions, so that the air outlets of the plurality of heat dissipation blocks 201 in two adjacent rows respectively discharge air in opposite directions, and the air outlets of the amplification modules 1 in each row are not affected by each other, so as to achieve the purpose of improving the heat dissipation efficiency.
Further, as shown in fig. 5 and 6, the heat dissipation block 201 further includes a first air guiding pipe 2013, wherein the top end of the first air guiding pipe 2013 is connected to the bottom edges of the plurality of heat dissipation fins 2011, so as to form an air inlet at the first air guiding pipe 2013, and cold air can be introduced into the gap between each two connected heat dissipation fins 2011 through the first air guiding pipe 2013, and carry heat to be discharged from an air outlet formed between two adjacent heat dissipation fins 2011 on the side opposite to the air guiding plate 2012.
In an alternative embodiment of the present invention, as shown in fig. 1 to 4, 7 and 8, the heat dissipation module 2 further includes an air duct 202 for providing cooling air to the plurality of heat dissipation blocks 201, the air duct 202 may be, but is not limited to, a cuboid shape, the air duct 202 has an air duct 2021 and a plurality of air passing holes communicating with the air duct 2021, one end of the air duct 202 is sealed, the other end of the air duct 202 has an inlet 2024 communicating with the air duct 2021 along the length direction of the air duct 202, and a fan 2022 is provided at the inlet 2024; at least part of the air passing holes are distributed on the air pipe 202 at intervals along the length direction of the air pipe 202, and the plurality of heat dissipation blocks 201 are respectively connected with the corresponding air passing holes (namely, each amplification module 1 corresponds to each air passing hole on the air pipe 202 one by one).
Further, as shown in fig. 1 to 4, 7 and 8, the heat dissipation module 2 further includes a plurality of second air guiding pipes 2023, where the plurality of second air guiding pipes 2023 are respectively disposed at the corresponding air passing holes and connected to the corresponding first air guiding pipes 2013. In use, the fan 2022 introduces external cold air into the air duct 2021 and flows along the extending direction of the air duct 2021 (i.e. the length direction of the air duct 202), and the plurality of air-passing holes are located on the upper surface of the air duct 202, and through the cooperation of the first air-guiding tube 2013 and the second air-guiding tube 2023, cold air can be led from the air duct 2021 to the second air-guiding tube 2023 and sequentially flow through the communicated air-passing holes and the first air-guiding tube 2013 and then enter the gaps between each two adjacent cooling fins 2011 in the cooling block 201, and due to the arrangement of the air-guiding plate 2012, the flow direction of air flow in the gaps between the two adjacent cooling fins 2011 is limited and guided, so that the air flow carries heat and is discharged from the air outlet in the preset direction.
Specifically, as shown in fig. 7 and 8, the second air induction pipe 2023 includes an air inlet section 20231 and an air outlet section 20232 that are connected, the cross-sectional area of the air inlet section 20231 is larger than that of the air outlet section 20232, the air inlet section 20231 is located in the air duct 2021, and the air outlet section 20232 passes through the corresponding air passing hole and extends into the corresponding first air induction pipe 2013. Wherein, the cross section area of the air inlet section 20231 is larger than that of the air outlet section 20232, so that cold air can enter conveniently; while the cross-sectional area and cross-sectional shape of the air outlet section 20232 need to be adapted to the first air induction pipe 2013 so that the air outlet section 20232 can be nested with the first air induction pipe 2013.
In an alternative embodiment of the present invention, as shown in fig. 3, 4, 9 and 10, the amplification module 1 includes a positioning member 101 for providing a placement position for the sample tube 3 and a heating and cooling module 104 for heating or cooling the sample tube 3 according to a replication and amplification stage of a sample to be tested, where the positioning member 101 is vertically arranged and has a tube shape, the positioning member 101 has a receiving cavity 1011, the sample tube 3 is located in the receiving cavity 1011, the heating and cooling module 104 is connected to the positioning member 101, and the heating and cooling module 104 is connected to the top of the corresponding heat dissipation block 201 in the heat dissipation module 2 through the positioning member 101. Wherein the positioning member 101 is made of a heat insulating material. The positioning piece 101 can play a role in positioning the sample tube 3 on one hand, so that the sample tube 3 can be placed in place quickly; on the other hand, the positioning piece 101 has heat insulation capacity, so that heat transmission between two adjacent amplification modules 1 can be prevented, independence of samples to be detected in two adjacent sample tubes 3 in the detection process is guaranteed, and the situation that the samples to be detected in different time opening detection experiments receive heat or lose heat at incorrect time due to heat transmission and influence the accuracy of detection results is avoided.
Further, the heating and cooling module 104 may employ, but is not limited to, peltier.
In this embodiment, as shown in fig. 3, 4, 9 and 10, the amplification module 1 further includes a heat transfer tube 102 and a heat transfer connection piece 103, wherein the heat transfer tube 102 includes a tube body 1021 and a plate-shaped connection portion 1022 disposed at the bottom of the tube body 1021; the heating and refrigerating module 104, the heat conducting connecting sheet 103 and the connecting part 1022 are sequentially connected from bottom to top, the tube 1021 is arranged in the accommodating cavity 1011 in a penetrating way, and the sample tube 3 can enter the accommodating cavity 1011 from the top opening of the accommodating cavity 1011 and be arranged in the tube 1021. When in use, the sample tube 3 is placed in the tube body 1021 of the heat transfer tube 102, and when the heating and refrigerating module 104 is heated, heat is conducted to a sample to be tested in the sample tube 3 through the heat transfer tube 102; when the heating and cooling module 104 cools down, heat is discharged from the sample tube 3 to the external environment through the heat sink 2011.
Further, the heat conducting connecting piece 103 may be, but is not limited to, a heat conducting film, wherein the top surface of the heat conducting film is attached to the bottom surface of the connecting portion 1022, and the bottom surface of the heat conducting film is attached to the top surface of the heating and refrigerating module 104.
When the sample tube 3 is arranged in the heat transfer tube 102, the sample to be measured in the sample tube 3 is just in the tube 1021, and external heat can be conducted from the periphery of the tube 1021 to the sample to be measured, so that the heating rate and the cooling rate of the sample to be measured are faster. The heat conducting film is attached between the heat transfer tube 102 and the heating and refrigerating module 104, so that the heat transfer efficiency can be improved through the heat conducting film, the heat conducting film is arranged because any one plane cannot form an absolutely smooth plane (the surface of the heat conducting film can be provided with fine pits and/or bulges), when the two planes are attached and connected with each other, gaps exist between the two planes and cannot be completely attached, and the heat transfer efficiency between the two planes can be reduced.
In an alternative embodiment of the present invention, as shown in fig. 3, 4, 9 and 10, the amplification module 1 further includes an optical fiber 105 for transmitting excitation light and emission light, where two ends of the optical fiber 105 are a first end and a second end, respectively, and the first end of the optical fiber 105 sequentially passes through the positioning element 101 and the tube body 1021 and extends to the position of the sample tube 3, and the second end of the optical fiber 105 is used for externally connecting an optical detection device, and the optical detection device is used for emitting excitation light and receiving the emission light generated by the sample to be tested. In the use process, the optical fiber 105 is used for transmitting excitation light and emission light between the sample tube 3 and the optical detection device, namely, the excitation light emitted by the optical detection device is transmitted to the sample to be detected in the sample tube 3 through the optical fiber 105, the sample to be detected is excited to generate emission light, the emission light generated by the sample to be detected is transmitted back to the optical detection device through the optical fiber 105, and the optical detection device is used for collecting, qualitatively and quantitatively analyzing the emission light, so that the amplification condition of the sample to be detected can be obtained. The optical detection device may be an existing optical detection device that may be used to emit excitation light and receive emitted light, and the specific structure of the optical detection device is not limited herein.
Further, as shown in fig. 9 and 10, a rectangular bump 1014 is formed on the outer wall of the positioning member 101, a first hole 1012 communicating with the accommodating channel 1011 is formed on the bump 1014, the extending direction of the first hole 1012 is perpendicular to the axial direction of the accommodating channel 1011, a second hole 1023 communicating with the inside of the first hole 1012 is formed on the tube 1021, the extending direction of the second hole 1023 is perpendicular to the axial direction of the tube 1021, when the tube 1021 is inserted into the accommodating channel 1011, the first hole 1012 is communicated with the second hole 1023, the first end of the optical fiber 105 can sequentially pass through the first hole 1012 and the second hole 1023 to extend into the tube 1021, and the optical fiber 105 is in contact with but not connected with the sample tube 3. The positioning piece 101 is provided with a clamp spring 106, and the connection position of the first end of the optical fiber 105 can be locked or released through the clamp spring 106, so that stable installation and convenient disassembly of the optical fiber 105 are ensured.
Specifically, as shown in fig. 9 and 10, the protrusion 1014 of the positioning element 101 is provided with a third hole 1013 penetrating through two opposite sidewalls of the protrusion 1014, the third hole 1013 is communicated with the first hole 1012, the extending direction of the third hole 1013 may be perpendicular to the extending direction of the first hole 1012 (i.e., the extending direction of the third hole 1013, the extending direction of the first hole 1012 and the axial direction of the tube 1021 may be directions of an X axis, a Y axis and a Z axis in a three-dimensional coordinate system respectively), the clamp spring 106 includes a clamping section, a fixed end 1061 and an actuating end 1062 at two ends of the clamping section,
the fixed end 1061 of the clamp spring 106 is fixedly connected to the outer wall of the positioning member 101, and the action end 1062 of the clamp spring 106 enters the third hole 1013 from one side of the third hole 1013, passes through the communication position between the third hole 1013 and the first hole 1012, and then extends out from the other side of the third hole 1013. At least part of the clamping section of the clamping spring 106 is positioned at the communication position of the third hole 1013 and the first hole 1012; the optical fiber 105 is provided with a concave portion 1051 at least at a portion near the first end, and the position of the actuating end 1062 of the clamp spring 106 is adjusted in the third hole 1013, so that at least a portion of the clamping section in the third hole 1013 can be driven to be clamped into the concave portion 1051 or be moved out of the concave portion 1051, thereby realizing locking or releasing operation on the connection position of the first end of the optical fiber 105. The concave portion 1051 may be an annular clamping groove provided along the circumferential direction of the optical fiber 105, or may be a segment of an arc clamping groove extending along the circumferential direction of the optical fiber 105, so that the clamping section of the clamping spring 106 can be clamped into the clamping groove, and the optical fiber 105 cannot fall off.
In the process of actually assembling the optical fiber 105, when the optical fiber 105 is installed, the action end 1062 of the clamp spring 106 is lifted up to lift the clamping section of the clamp spring 106, so that the clamping section of the clamp spring 106 can avoid a certain space for the optical fiber 105, the first end of the optical fiber 105 sequentially passes through the first hole 1012 and the second hole 1023 to extend into the tube 1021, then the action end 1062 of the clamp spring 106 can be released, the clamping section of the clamp spring 106 is clamped into the concave part 1051 on the optical fiber 105 under the action of restoring force, the freedom degree of the optical fiber 105 in the axial direction of the optical fiber is limited, and the optical fiber 105 is fixed on the positioning piece 101; when the optical fiber 105 needs to be disassembled, the action end 1062 of the clamp spring 106 is lifted up, so that the clamping section of the clamp spring 106 is removed from the concave portion 1051 on the optical fiber 105 and separated, and the optical fiber 105 can be pulled out, and the disassembly of the optical fiber 105 is completed. The installation structure of the optical fiber 105 has the advantages of convenience in installation and disassembly of the optical fiber 105, is simple and convenient to operate, is convenient for workers to manually install and disassemble the optical fiber 105, is more convenient and quick compared with the traditional adhesive fixing mode, and is also beneficial to replacement of the optical fiber 105.
In an alternative embodiment of the present invention, as shown in fig. 1 and 2, the temperature control device for a PCR instrument further includes a plurality of light shielding covers 4, where the plurality of light shielding covers 4 are in one-to-one correspondence with the plurality of amplification modules 1, and the plurality of light shielding covers 4 are respectively disposed above the corresponding amplification modules 1. After the sample tube 3 is placed in the positioning piece 101, a shading cover 4 is covered above the sample tube 3 to prevent external environment light from being injected into the sample tube 3, so that the influence on the detection of a sample to be detected is avoided.
In another alternative embodiment of the present invention, as shown in FIG. 11, a plurality of amplification modules 1 are arranged in a plurality of rows in the horizontal direction; corresponding to the multiple rows of amplification modules 1, the heat dissipation module 2 comprises a plurality of air channels 2021, two adjacent air channels 2021 are separated by a partition board 5, and each row of amplification modules 1 is respectively located above the corresponding air channel 2021. Through the setting of baffle 5, can with cold air reposition of redundant personnel to in many wind channels 2021, further promoted the independence of single amplification module 1 in the use intensification, cooling, reduce the heat transmission between the adjacent amplification module 1, improve the control by temperature change effect of single amplification module 1, promote the accuracy that detects.
In yet another alternative embodiment of the present invention, as shown in fig. 12, the heat dissipation module 2 is a whole heat dissipation structure formed by arranging a plurality of heat dissipation fins 2011 side by side along a vertical direction, one end of each heat dissipation fin 2011 is provided with a fan 2022, and a plurality of expansion modules 1 are located on top of the heat dissipation module 2. In this embodiment, the plurality of amplification modules 1 share one heat radiation structure, so that the integration is good, and the disassembly and assembly are convenient. One fan 2022 or a plurality of fans 2022 may be provided according to the heat dissipation effect, so as to ensure that a good heat dissipation effect is achieved.
The temperature control device for the PCR instrument has the characteristics and advantages that:
1. according to the temperature control device for the PCR instrument, under the condition that the number of samples to be detected is huge and the samples cannot be detected at the same time, the samples to be detected can be detected at the same time, the detection experiment of the samples to be detected does not need waiting, so that the speed of outputting the detection result of the samples to be detected is increased, and meanwhile, the experimental process of the samples to be detected which are detected at first cannot be influenced.
2. In the temperature control device for the PCR instrument, the plurality of radiating blocks 201 are matched with the air pipes 202, and the plurality of radiating blocks 201 are in one-to-one correspondence with the plurality of amplifying modules 1, so that independent heat dissipation and cooling can be carried out on each amplifying module 1, the influence among the amplifying modules is reduced, and the heat dissipation efficiency is improved; in addition, the air duct 202 provides a uniform source of cool air for the plurality of heat dissipation blocks 201, and guides cool air to enter the corresponding heat dissipation blocks 201 respectively and then to be discharged from a designated direction, thereby improving heat dissipation efficiency.
3. In the temperature control device for the PCR instrument, the structure of the amplification module 1 can avoid the mutual influence of samples to be detected in two adjacent sample tubes 3, ensure the accuracy of detection results, effectively improve the heat conduction efficiency and provide a better detection environment for the samples to be detected.
4. In this temperature control device for PCR appearance, carry out the joint to optic fibre 105 through jump ring 106, can realize the locking and the release of optic fibre 105, easy operation, make things convenient for the installation, the dismantlement of optic fibre 105, compare in the fixed mode of traditional sticky, more convenient and fast, also do benefit to the change of optic fibre 105.
Second embodiment
The invention provides a PCR instrument, which is provided with the temperature control device for the PCR instrument.
The PCR instrument has the characteristics and advantages of the temperature control device for the PCR instrument, and the details are not repeated here.
The foregoing is illustrative of the present invention and is not to be construed as limiting the scope of the invention. Any equivalent changes and modifications can be made by those skilled in the art without departing from the spirit and principles of this invention, and are intended to be within the scope of this invention.
Claims (15)
1. The temperature control device for the PCR instrument is characterized by comprising:
a plurality of amplification modules, wherein the amplification modules are configured to respectively place sample tubes for storing samples to be tested, and the amplification modules are used for respectively carrying out copying and amplification treatment on the samples to be tested, which are added into the corresponding sample tubes;
the heat dissipation module is connected with at least one amplification module and is used for assisting the amplification module in heat dissipation and cooling when the amplification module is cooled;
the amplification module comprises a positioning piece for providing a placement position for the sample tube, a heating and refrigerating module for heating or cooling the sample tube according to the replication and amplification stage of the sample to be detected, a heat transfer tube and a heat conduction connecting piece, wherein the positioning piece is vertically arranged in a tubular shape, a containing cavity is formed in the positioning piece, the sample tube is positioned in the containing cavity, the heating and refrigerating module is connected with the positioning piece, the heating and refrigerating module is connected to the top of the heat dissipation module through the positioning piece, and the positioning piece is made of a heat insulation material;
the heat transfer tube comprises a tube body and a platy connecting part arranged at the bottom of the tube body; the heating and refrigerating module, the heat conduction connecting sheet and the connecting part are sequentially connected from bottom to top, the tube body penetrates through the accommodating cavity, the sample tube can enter the accommodating cavity from the top opening of the accommodating cavity and is placed in the tube body, and when the heating and refrigerating module heats up, heat is conducted to a sample to be tested in the sample tube through the heat transfer tube; when the heating and refrigerating module cools down, heat is discharged from the sample tube to the external environment through the heat radiation module;
the top surface of heat conduction connection piece with the bottom surface of connecting portion is laminated mutually, the bottom surface of heat conduction connection piece with the top surface of heating and refrigerating module is laminated mutually.
2. The temperature control device for a PCR instrument according to claim 1, wherein the heat dissipation module comprises a plurality of heat dissipation blocks, and the plurality of heat dissipation blocks are connected with the plurality of amplification modules in a one-to-one correspondence manner so as to conduct heat dissipation and temperature reduction on the corresponding amplification modules through the plurality of heat dissipation blocks.
3. The temperature control device for a PCR instrument according to claim 2, wherein the heat dissipating block includes a plurality of heat dissipating fins and an air guiding plate arranged side by side, and the air guiding plate is connected to the heat dissipating fins so as to form an air outlet between the two connected heat dissipating fins on a side opposite to the air guiding plate.
4. The temperature control device for a PCR instrument according to claim 3, wherein the plurality of amplification modules are arranged in a plurality of rows in the horizontal direction, and the air deflectors corresponding to the amplification modules in each row have the same arrangement direction, so that the air outlets of the plurality of heat dissipation blocks in each row are led out in the same direction;
the air deflectors corresponding to the amplification modules in each row have different setting directions, so that the air outlets of the plurality of heat dissipation blocks in two adjacent rows respectively emit air in opposite directions.
5. The temperature control device for a PCR instrument according to any one of claims 3 to 4, wherein the heat sink further includes a first air introduction pipe connected to the heat sink to form an air intake at the first air introduction pipe.
6. The temperature control device for a PCR instrument according to claim 5, wherein the heat dissipating module comprises an air duct for supplying cooling air to the plurality of heat dissipating blocks, the air duct having an air duct and a plurality of air passing holes communicating with the air duct, the plurality of heat dissipating blocks being connected to the corresponding air passing holes, respectively.
7. The temperature control device for a PCR instrument according to claim 6, wherein the heat dissipation module further comprises a plurality of second air guiding pipes, and the plurality of second air guiding pipes are respectively arranged at the corresponding air passing holes;
the second induced air pipe comprises an air inlet section and an air outlet section which are communicated, the air inlet section is positioned in the air duct, and the air outlet section penetrates through the corresponding air passing hole and stretches into the corresponding first induced air pipe.
8. The temperature control device for the PCR instrument according to claim 6, wherein one end of the air pipe is sealed, the other end of the air pipe is provided with an inlet communicated with the air duct along the length direction of the air pipe, and a fan is arranged at the inlet;
at least part of the air passing holes are distributed on the air pipe at intervals along the length direction of the air pipe.
9. The temperature control device for a PCR instrument according to claim 1, wherein the amplification module further comprises an optical fiber for transmitting excitation light and emission light, a first end of the optical fiber sequentially passes through the positioning member and the tube body and extends to a position where the sample tube is located, and a second end of the optical fiber is used for externally connecting an optical detection device, and the optical detection device is used for emitting the excitation light and receiving the emission light generated by the sample to be measured.
10. The temperature control device for the PCR instrument according to claim 9, wherein the positioning piece is provided with a first hole communicated with the accommodating cavity, the pipe body is provided with a second hole communicated with the inside of the pipe body, when the pipe body is arranged in the accommodating cavity in a penetrating manner, the first hole is communicated with the second hole, and the first end of the optical fiber sequentially penetrates through the first hole and the second hole and stretches into the pipe body;
and the positioning piece is provided with a clamp spring which is used for locking or releasing the connection position of the first end of the optical fiber.
11. The temperature control device for the PCR instrument according to claim 10, wherein a third hole communicated with the first hole is formed in the positioning piece, the fixed end of the clamp spring is connected to the positioning piece, and the action end of the clamp spring enters the third hole from one side of the third hole and extends out from the other side of the third hole after passing through the communication position of the third hole and the first hole;
and a concave part is arranged on the optical fiber and close to at least part of the first end, and the position of the action end of the clamp spring is adjusted in the third hole so as to drive at least part of the clamp spring in the third hole to be clamped into the concave part or moved out of the concave part.
12. The temperature control device for a PCR instrument according to claim 10, further comprising a plurality of light shielding covers, wherein the plurality of light shielding covers are respectively arranged above the corresponding amplification modules.
13. The temperature control device for a PCR instrument according to claim 5, wherein the plurality of amplification modules are arranged in a plurality of rows in a horizontal direction;
the heat dissipation module comprises a plurality of air channels, two adjacent air channels are separated through a partition board, and each row of amplification modules are respectively located above the corresponding air channels.
14. The temperature control device for the PCR instrument according to claim 1, wherein the heat dissipation module is an integral heat dissipation structure formed by arranging a plurality of heat dissipation fins side by side, a fan is arranged at one end of each heat dissipation fin, and a plurality of the amplification modules are positioned at the top of the heat dissipation module.
15. A PCR instrument comprising a temperature control device according to any one of claims 1 to 14.
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| CN202311723619.0A CN117402731B (en) | 2023-12-14 | 2023-12-14 | Temperature control device for PCR instrument and PCR instrument |
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| CN202311723619.0A CN117402731B (en) | 2023-12-14 | 2023-12-14 | Temperature control device for PCR instrument and PCR instrument |
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| EP3953041A4 (en) * | 2019-04-08 | 2023-01-25 | Miroculus Inc. | Multi-cartridge digital microfluidics apparatuses and methods of use |
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| CN104619622A (en) * | 2012-07-31 | 2015-05-13 | 简·探针公司 | System, method, and apparatus for automated incubation |
| CN202973649U (en) * | 2012-10-26 | 2013-06-05 | 北京众驰伟业科技发展有限公司 | Inclined reagent cold storage tank for full-automatic blood coagulation tester |
| CN211720958U (en) * | 2020-02-18 | 2020-10-20 | 上海宏滩生物科技有限公司 | Novel gene amplification instrument heat dissipation device |
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