CN115806873A - Digital PCR amplification equipment - Google Patents
Digital PCR amplification equipment Download PDFInfo
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- CN115806873A CN115806873A CN202211472638.6A CN202211472638A CN115806873A CN 115806873 A CN115806873 A CN 115806873A CN 202211472638 A CN202211472638 A CN 202211472638A CN 115806873 A CN115806873 A CN 115806873A
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Abstract
The invention discloses a digital PCR amplification device, which comprises a heat preservation module for keeping constant temperature during nucleic acid amplification; a temperature sensor for detecting the temperature in real time during nucleic acid amplification; a membrane sealing module for sealing membrane and separating sample before sample amplification; a base plate for supporting the entire apparatus, maintaining the apparatus in a horizontal state; a heating block for heat transfer; a heating and refrigerating device for heating and cooling during nucleic acid amplification; a sample rack for storing and transporting samples. The temperature sensor is adopted in the invention, so that the uniformity of the temperature of the sample can be kept; adopt heating refrigerating plant can be better control the temperature, practice thrift the amplification time, the size of change sample frame can improve sample amplification efficiency, seals the membrane module and can realize preventing cross contamination, and the whole equipment volume ratio is less, but has high flux, and the compatibility is extremely strong, can integrate on an instrument, prevents that the sample needs continuous transfer at nucleic acid detection's in-process.
Description
Technical Field
The invention relates to the technical field of digital PCR, in particular to digital PCR amplification equipment.
Background
With the development of biotechnology, and the application of PCR technology in various fields, including medical disease detection, agricultural transgene detection and many other fields, the Polymerase Chain Reaction (PCR) is a molecular biology technology for amplifying and amplifying specific DNA fragments, which can be regarded as the special DNA replication in vitro, and the biggest characteristic of PCR is that trace amount of DNA can be greatly increased. Currently, for nucleic acid amplification techniques, more precise temperature control is required, and simultaneous amplification of large batches of samples is required.
However, the prior art has the following problems: firstly, the inaccurate and uneven temperature control in the nucleic acid amplification process leads to inaccurate test results, and the nucleic acid amplification cost on the market is high, so that the nucleic acid amplification process is not beneficial to large-scale amplification; secondly, the amplification instrument is incompatible with the previous and subsequent extraction and detection links, and is not well integrated into one instrument, and the amplification of the sample is performed in the presence of cross contamination or in advance by sealing a membrane on another instrument.
Disclosure of Invention
The invention mainly aims to provide a digital PCR amplification device, aiming at solving the problems of inaccurate test result and sample cross contamination caused by low and non-uniform temperature control in the nucleic acid amplification process.
To achieve the above object, the present invention provides a digital PCR amplification apparatus, comprising: an incubation module for maintaining a constant temperature during nucleic acid amplification; a temperature sensor for detecting temperature in real time during nucleic acid amplification; a membrane sealing module for sealing membrane and separating sample before sample amplification; a base plate for supporting the entire apparatus, maintaining the apparatus in a horizontal state; a heating block for heat transfer; a heating and refrigerating device for heating and cooling during nucleic acid amplification; a sample rack for storing and carrying samples; the heat preservation module is installed on the bottom plate, the film sealing module is installed on the heat preservation module, the heating block is arranged between the heat preservation module and the film sealing module, the temperature sensor is arranged inside the heating block, the heating and refrigerating device is arranged at the bottom of the heating block, the sample frame is arranged on the heating block, and the heating block is used for transferring heat of the heating and refrigerating device to the sample frame to heat and cool the sample.
In one embodiment, the digital PCR amplification device further comprises a heat dissipation device, wherein the heat dissipation device comprises a heat dissipation fin mounting frame and heat dissipation fins, the heat dissipation fins are arranged on the heat dissipation fin mounting frame, the heating and cooling device is arranged on the heat dissipation fins, and air ducts are arranged on two sides of the heat dissipation fin mounting frame.
In one embodiment, a support frame is arranged at the bottom of the heat sink mounting frame, the support frame is arranged on the bottom plate, a fan mounting frame is arranged on one side of the air duct, and a fan is arranged inside the support frame and arranged on the bottom plate.
In one embodiment, the heat preservation module comprises a heat preservation cover and heat preservation bakelite, the heat preservation bakelite is arranged on the cooling fin installation rack and located on two sides of the heating block, the heat preservation cover is connected with the heat preservation bakelite in a sliding mode, and the heat preservation cover slides back and forth towards the heat preservation bakelite.
In one embodiment, a heating film upper cover, a thermal cover and a lead screw motor connecting block are arranged inside the heat preservation cover, the lead screw motor connecting block is arranged above the heating film upper cover, the heating film upper cover is arranged above the heating film, and the thermal cover is arranged at the bottom of the heating film upper cover.
In one embodiment, a spring and a fixing plate are further arranged above the heat-insulating cover, one end of the spring is connected with the heat cover, and the other end of the spring is connected with the fixing plate.
In one embodiment, a screw motor, a first linear bearing, a second linear bearing, a first guide post and a second guide post are arranged on the fixing plate, the first bearing and the second bearing are arranged on two sides of the screw motor, the first guide post penetrates through the first bearing and the fixing plate, one end of the first guide post is connected with the hot cover, the second guide post penetrates through the second bearing and the fixing plate, one end of the second guide post is connected with the hot cover, and the screw motor penetrates through a screw motor connecting block to be connected with the upper cover of the heating film.
In one embodiment, support blocks are arranged on two sides of the bottom plate, a support beam is arranged on one side of the fixing plate, one end of the support beam is supported on the fixing plate, and the other end of the support beam is supported on the support blocks.
In one embodiment, when the heat preservation cover moves above the heat preservation bakelite, the heat preservation cover is in contact with the heat preservation bakelite, a cavity is formed between the heat preservation cover and the heat preservation bakelite in an enclosing manner, and a plurality of temperature sensor mounting holes are formed in the heating and refrigerating device.
In one embodiment, an aluminum film groove is formed in the heat-insulating cover, an aluminum film used for sealing the film is placed in the aluminum film groove, and a temperature detection board card is arranged on one side of the outer portion of the heat-insulating cover.
The invention has the beneficial effects that:
the digital PCR amplification equipment provided by the invention is provided with the plurality of temperature sensors, so that the temperature change of each position can be accurately monitored in real time, temperature overshoot is prevented, and the integrity of a sample in the amplification process can be effectively maintained; adopt heating refrigerating plant with heat transfer to the heating block, transmit the temperature to the sample frame by the heating block again, sample to on the sample frame heaies up, the cooling, can control the rate that the temperature rises or descends, can be better control the temperature, practice thrift the amplification time, change the structure size of sample frame can realize a plurality of samples and amplify simultaneously, improve sample amplification efficiency, seal the membrane module and can realize sealing the membrane to the sample frame before the sample is amplified, prevent cross contamination, whole equipment is all littleer than the current product on the market, but have high flux and stable temperature control, and compatibility is extremely strong, can integrate on an instrument, prevent that the sample needs continuous transfer at nucleic acid testing's in-process.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the structures shown in the drawings without creative efforts.
FIG. 1 is a schematic diagram of the digital PCR amplification apparatus according to the present invention;
FIG. 2 is a cross-sectional view of the digital PCR amplification apparatus of the present invention;
FIG. 3 is a schematic view of the structure of the cavity of the present invention;
FIG. 4 is a view showing the placement of an aluminum film of the present invention;
fig. 5 is a hole bit map of the temperature sensor of the present invention.
The reference numbers illustrate:
a base plate 11; a support frame 12; a support block 13; a heat sink mounting frame 14; a support beam 15; a fixed plate 16; a first linear bearing 17; a first guide post 18; a screw motor 19; a second linear bearing 20; the second guide post 21; a spring 22; a heat-insulating cover 23; a heating block 24; a heat sink 25; a heat preservation bakelite 26; an air duct 27; a fan mounting frame 28; a fan 29; a temperature detection board card 30; a lead screw motor connecting block 31; the heating film 32; a heating film upper cover 33; a thermal cover 34; a heating and cooling device 35; a sample rack 36; a cavity 37; an aluminum film 38; an aluminum film groove 39; a temperature sensor mounting hole 40.
The implementation, functional features and advantages of the present invention will be further described with reference to the accompanying drawings.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without inventive step based on the embodiments of the present invention, are within the scope of protection of the present invention.
It should be noted that, if directional indications (such as upper, lower, left, right, front, rear, 8230; \8230;) are involved in the embodiment of the present invention, the directional indications are only used to explain the relative positional relationship between the components in a specific posture (as shown in the figure), the motion situation, etc., and if the specific posture is changed, the directional indications are correspondingly changed.
In addition, if there is a description relating to "first", "second", etc. in the embodiments of the present invention, the description of "first", "second", etc. is for descriptive purposes only and is not to be construed as indicating or implying relative importance or to implicitly indicate the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In addition, if appearing throughout the text, "and/or" is meant to include three juxtaposed aspects, taking "A and/or B" as an example, including either the A aspect, or the B aspect, or both A and B satisfied aspects. In addition, technical solutions between the embodiments may be combined with each other, but must be based on the realization of the technical solutions by a person skilled in the art, and when the technical solutions are contradictory to each other or cannot be realized, such a combination should not be considered to exist, and is not within the protection scope of the present invention.
Referring to fig. 1 to 5, a digital PCR amplification apparatus according to an embodiment of the present invention includes: an incubation module for maintaining a constant temperature during nucleic acid amplification; a temperature sensor (not shown) for detecting the temperature in real time during nucleic acid amplification; a membrane sealing module for sealing membrane and separating sample before sample amplification; a base plate 11 for supporting the entire apparatus, maintaining the apparatus in a horizontal state; a heating block 24 for heat transfer; a heating and cooling device 35 for heating and cooling during nucleic acid amplification; a sample rack 36 for storing and carrying samples; wherein, the heat preservation module install in on the bottom plate 11, seal membrane module install in on the heat preservation module, heating block 24 set up in the heat preservation module with seal between the membrane module, temperature sensor (not shown in the figure) set up in inside the heating block 24, heating refrigerating plant 35 set up in heating block 24 bottom, sample frame 36 place in on the heating block 24, the heating block 24 be used for with the heat transfer of heating refrigerating plant 35 arrives on the sample frame 36, heat up, cool down the sample.
The incubation module is provided with one or more than one, and has the functions of keeping each sample position incubated during nucleic acid amplification and keeping the uniformity of the sample position, so that the sample temperature at different positions is the same and is kept constant.
One or more samples are when carrying out the simultaneous amplification, and are a plurality of temperature sensor (not shown in the figure) can each temperature of point of real-time supervision, makes temperature control, ensures that the sample when the amplification, and the temperature on the difference can be adjusted according to temperature sensor (not shown in the figure)'s feedback, and then guarantees the temperature homogeneity of every amplification point, ensures the effect of amplification to guarantee the detection accuracy of follow-up detection link, increase temperature sensor (not shown in the figure) or change the position of temperature sensor (not shown in the figure), also can reach the monitoring purpose, make this structure have more the flexibility.
The sample separation module is provided with one or more modules, cross infection risks easily exist in the process of amplification of each sample, the membrane sealing module can seal membranes before amplification of each sample, different samples can be separated from each other in the process of amplification, pollution cannot be caused, the membrane sealing function can be realized before nucleic acid amplification of the samples, the samples do not need to be manually transferred to other instruments for membrane sealing (membrane sealing is carried out after amplification in the traditional method), and then the samples are manually transferred back to the amplified modules or instruments for nucleic acid amplification, so that the operation is greatly facilitated, and the pollution risks are reduced.
The heating and refrigerating devices 35 can be set to be Peltier, a plurality of heating and refrigerating devices 35 heat samples at the same time, and the rising and falling speed of the temperature can directly influence the amplification effect during nucleic acid amplification, the heating and refrigerating devices 35 are adopted to heat the samples at the same time, and the rising or falling speed can reach 4 ℃ per second in the temperature range of 0-70 ℃; in the range of 70-95 deg.c, the rate of rise or fall may be up to 2 deg.c per second. The effect can make the temperature control better and save the amplification time. When the temperature exceeds 95 ℃, the sample can be damaged, so that the temperature does not exceed the required temperature while the temperature rises, the temperature rise and fall rate of the amplification process is extremely critical, and the temperature rise rate falls in the high temperature, so that the temperature interval can be controlled more accurately, and overshoot is prevented.
Referring to fig. 1, digital PCR amplification equipment still includes heat abstractor, heat abstractor includes fin installation frame 14, fin 25 set up in groove on the fin installation frame 14 is convenient for fix the position of fin 25 makes fin 25 is fixed in on the fin installation frame 14, difficult removal, landing, heating refrigerating plant 35 set up in on the fin 25, it is a plurality of when heating refrigerating plant 35 simultaneous working, its refrigeration or heat the face with fin 25 contact will unnecessary energy release, fin installation frame 14 both sides are provided with wind channel 27, are convenient for the outside air of 14 both sides of fin installation frame enters into inside the fin installation frame 14, wind channel 27 still has and is used for heating refrigerating plant 35 keeps refrigerating or heats the homothermal function of face. In the amplification process, the heating and cooling device 35 is away from the sample side, which may generate unnecessary cooling or heating function, and at this time, it is necessary to discharge the opposite energy to the air to prevent the cooling or heating effect generated during amplification from affecting the amplification effect.
Further, as shown in fig. 1, a supporting frame 12 is disposed at the bottom of the heat sink mounting frame 14, the supporting frame 12 is disposed on the bottom plate 11, a fan mounting frame 28 is disposed at one side of the air duct 27, a fan 29 is disposed inside the supporting frame 12, the energy released by the heat sink 25 continuously draws out the air through the fan 29, a space with lower air pressure is formed inside the heat sink mounting frame 14, at this time, the air in the external space enters the inside of the heat sink mounting frame 14 from both sides of the heat sink 25, and is then drawn out by the fan 29, and continuously circulates back and forth to form an air flow channel, so as to take away the excess energy on the heat sink 25, intake air enters from both sides of the heat sink mounting frame 14, and then the heat passing through the heat sink 25 is dissipated out of the external air by the fan 29.
Referring to fig. 1, the heat preservation module includes a heat preservation cover 23 and a heat preservation bakelite 26, the heat preservation bakelite 26 is disposed on the heat sink mounting frame 14 and disposed on two sides of the heating block 24, the heat preservation cover 23 is movably connected to the heat preservation bakelite 26, the heat preservation cover 23 moves back and forth in a direction toward the heat preservation bakelite 26 to provide a movement basis for a subsequent film sealing operation, when the heat preservation cover 23 moves downward in a direction toward the heat preservation bakelite 26, the device can perform a film sealing operation on the sample rack 36 placed on the heating block 24, when the heat preservation cover 23 moves upward in a direction opposite to the heat preservation bakelite 26, the heat preservation cover 23 is raised to a proper height to cool down, when the temperature is lowered to 105 ℃, the heat preservation cover 23 moves downward again in a direction toward the heat preservation bakelite 26, and the movement during the film sealing is repeated to perform a heating process on the upper surface of the sample rack 36.
Further, as shown in fig. 2, a heating film 32, a heating film upper cover 33, a heat cover 34 and a lead screw motor connecting block 31 are arranged inside the heat-insulating cover 23, the lead screw motor connecting block 31 is arranged above the heating film upper cover 33, the heating film upper cover 33 is arranged above the heating film 32, the heat cover 34 is arranged at the bottom of the heating film upper cover 33, the heating film 32 is arranged inside the heat cover 34, a spring 22 and a fixing plate 16 are further arranged above the heat-insulating cover 23, one end of the spring 22 is connected with the heating film 32, the heating film upper cover 33 and the heat cover 34, the other end is connected with the fixing plate 16, when the heat-insulating cover 23 moves downwards towards the heat-insulating bakelite 26, the heat preservation cover 23, the heating film 32, the heating film upper cover 33, the heat cover 34 and the lead screw motor connecting block 31 arranged inside the heat preservation cover 23 also move downwards together, at this time, the heating film 32 is at 195 ℃, the heat preservation cover 23 is firstly contacted with the heat preservation bakelite 26 along with continuous descending, at this time, the heat preservation cover 23 cannot move downwards continuously, but the heating film upper cover 33, the heating film 32, the heat cover 34 and the like are connected through the spring 22, so that the downward movement can be continuously carried out at this time, the spring 22 is compressed, the heating film upper cover 33, the heating film 32, the heat cover 34 and the like continuously move downwards, and are attached to the sample rack 36 arranged on the heating block 24, and the effect of film sealing is achieved.
Furthermore, referring to fig. 2, a screw motor 19, a first linear bearing 17, a second linear bearing 20, a first guide post 18, and a second guide post 21 are disposed on the fixing plate 16, the first bearing and the second bearing are disposed on two sides of the screw motor 19, the first guide post 18 passes through the first linear bearing 17 and the fixing plate 16, the first guide post 18 is connected to the heat cover 34, the second guide post 21 passes through the second linear bearing 20 and the fixing plate 16, the second guide post 21 is connected to the heat cover 34, the screw motor 19 passes through the screw motor connecting block 31 and is connected to the heating film upper cover 33, and the first linear bearing 17, the second linear bearing 20, the first guide post 18, and the second guide post 21 provide an upward or downward force during the operation of the screw motor 19, so as to limit the motion trajectory, and ensure that the heat-insulating cover 23 moves to and fro in the direction of the heat-insulating electric wood 26.
Referring to fig. 1, the bottom plate 11 is provided with support blocks 13 on both sides thereof, the fixed plate 16 is provided with a support beam 15 on one side thereof, one end of the support beam 15 is connected to the fixed plate 16, and the other end of the support beam 15 is connected to the support blocks 13, and the support blocks are arranged on both sides of the bottom plate 11 to support objects inside the whole device, thereby maintaining the stability of the device.
Referring to fig. 3, the heat-insulating cover 23 moves above the heat-insulating bakelite 26, the heat-insulating cover 23 is connected to the heat-insulating bakelite 26, a cavity 37 is formed between the heat-insulating cover 23 and the heat-insulating bakelite 26, the cavity 37 is a closed space formed for the sample rack 36 placed on the heating block 24, and is used for preventing heat dissipation, air inside the cavity 37 is heated by the heating film 32, so that the temperatures of the samples at the positions of the sample rack 36 are the same, temperature uniformity is ensured, and the heating and cooling device 35 is provided with a plurality of temperature sensor (not shown in the figure) mounting holes 40, so that the temperatures of the cavity 37 and the heating and cooling device 35 are monitored in real time.
Referring to fig. 4, the inside aluminium membrane groove 39 that is provided with of heat preservation lid 23, aluminium membrane 38 that is used for follow-up membrane that seals has been placed to aluminium membrane groove 39 inside, before placing on heating block 24 sample frame 36 can place an aluminium membrane 38 in the aluminium membrane groove 39, be used for the follow-up the membrane operation of sealing of sample frame 36, aluminium membrane 38 can place before whole module starts in aluminium membrane groove 39 steadily wait to use, the outside one side of heat preservation lid 23 is provided with temperature detection integrated circuit board 30, the temperature that temperature sensor (not shown in the figure) gathered can real-time transmission to on the temperature detection integrated circuit board 30, the rethread external computer shows, makes operating personnel can master the change of temperature in real time.
The digital PCR amplification equipment is an independent module, has high compatibility, can be compatible to different instruments, and is used as one core amplification module for amplification. When the nucleic acid extraction module is integrated into an extraction, amplification and detection integrated instrument, a nucleic acid detection integrated instrument with comprehensive functions can be formed, so that the module is used as an independent module, has strong compatibility, is matched with a plurality of instruments, can be integrated and designed into an automatic nucleic acid detection integrated instrument, and is connected with extraction and detection in series, thereby reducing the risk caused by manual operation. In the existing nucleic acid integrated instrument, a large-flux instrument is often in the form of a single module, and the instrument occupies a large space. The invention has the advantages of smaller size, width and height than the existing products in the market, only occupies a small part of the volume, but has extremely high flux and stable temperature control. Through simple series connection, a complete full-automatic integrated nucleic acid detection instrument can be formed with a plurality of front and rear modules, and the occupied space is greatly reduced.
The heat preservation module of this embodiment uses the heat preservation lid 23 with the bakelite 26 of heat preservation passes through the motion of lead screw motor 19, and its effect is for preventing heat from running off from the top and stopping for the nucleic acid amplification in-process the condition that the upper portion of sample frame 36 is different with lower part temperature. In the actual design process, the lead screw motor 19 can be replaced by a common stepping motor, the heat preservation cover 23 is replaced by other heat preservation materials, the processing depth and different positions of the aluminum film groove 39 are changed, and the power, size, limit temperature and the like of the heating film 32 are changed to realize heat preservation. In addition, the screw motor 19 can be changed into a hinge, and the whole heat preservation and film sealing module can be used as a box cover type, so that the purpose of the invention can be achieved.
In specific implementation, the models of the plurality of temperature sensors (not shown in the figure) can be changed into other sensor models with more accurate precision, so that the temperature control of the invention is more accurate. And the amplification process can also be completed by changing the heating and cooling device 35 into other heating devices.
The film sealing function of this embodiment may be to place the aluminum film 38 on the sample holder 36 in advance, then cancel the four downward-pressing springs 22, do not pre-press the springs 22, directly attach the heat cover 34, and also perform the film sealing function.
The above description is only an alternative embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications and equivalents of the present invention, which are made by the contents of the present specification and the accompanying drawings, or directly/indirectly applied to other related technical fields, are included in the scope of the present invention.
Claims (10)
1. A digital PCR amplification apparatus, comprising:
an incubation module for maintaining a constant temperature during nucleic acid amplification;
a temperature sensor for detecting temperature in real time during nucleic acid amplification;
a membrane sealing module for sealing membrane and separating sample before sample amplification;
a base plate for supporting the entire apparatus, maintaining the apparatus in a horizontal state;
a heating block for heat transfer;
a heating and refrigerating device for heating and cooling during nucleic acid amplification;
a sample rack for storing and transporting samples;
the heat preservation module is installed on the bottom plate, the film sealing module is installed on the heat preservation module, the heating block is arranged between the heat preservation module and the film sealing module, the temperature sensor is arranged inside the heating block, the heating and refrigerating device is arranged at the bottom of the heating block, the sample frame is arranged on the heating block, and the heating block is used for transferring heat of the heating and refrigerating device to the sample frame to heat and cool the sample.
2. The digital PCR amplification apparatus according to claim 1, further comprising a heat dissipation device, wherein the heat dissipation device comprises a heat sink mounting frame and a heat sink, the heat sink is disposed on the heat sink mounting frame, the heating and cooling device is disposed on the heat sink, and air ducts are disposed on two sides of the heat sink mounting frame.
3. The digital PCR amplification apparatus of claim 2, wherein a support frame is disposed at the bottom of the heat sink mounting frame, the support frame is disposed on the bottom plate, a fan mounting frame is disposed at one side of the air duct, and a fan is disposed inside the support frame, the fan being disposed on the bottom plate.
4. The digital PCR amplification apparatus of claim 2, wherein the thermal module comprises a thermal cover and a thermal bakelite, the thermal bakelite is disposed on the heat sink mounting frame and located at two sides of the heating block, the thermal cover is slidably connected to the thermal bakelite, and the thermal cover slides back and forth towards the thermal bakelite.
5. The digital PCR amplification apparatus of claim 4, wherein the heat-preserving cover is internally provided with a heating film, a heating film upper cover, a heat cover and a lead screw motor connecting block, the lead screw motor connecting block is arranged above the heating film upper cover, the heating film upper cover is arranged above the heating film, and the heat cover is arranged at the bottom of the heating film upper cover.
6. The digital PCR amplification apparatus of claim 5, wherein a spring and a fixing plate are further disposed above the heat-insulating cover, one end of the spring is connected to the heat-insulating cover, and the other end of the spring is connected to the fixing plate.
7. The digital PCR amplification apparatus of claim 6, wherein the fixing plate is provided with a screw motor, a first linear bearing, a second linear bearing, a first guide post and a second guide post, the first bearing and the second bearing are disposed on two sides of the screw motor, the first guide post passes through the first bearing and the fixing plate, one end of the first guide post is connected with the heat cover, the second guide post passes through the second bearing and the fixing plate, one end of the second guide post is connected with the heat cover, and the screw motor passes through the screw motor connecting block and is connected with the upper cover of the heating membrane.
8. The digital PCR amplification apparatus of claim 6, wherein support blocks are provided at both sides of the base plate, a support beam is provided at one side of the fixing plate, one end of the support beam is supported on the fixing plate, and the other end of the support beam is supported on the support blocks.
9. The digital PCR amplification apparatus of claim 4, wherein the heat-preserving cover contacts the heat-preserving bakelite when the heat-preserving cover moves above the heat-preserving bakelite, a cavity is defined between the heat-preserving cover and the heat-preserving bakelite, and a plurality of temperature sensor mounting holes are arranged on the heating and cooling device.
10. The digital PCR amplification apparatus of claim 4, wherein an aluminum film groove is arranged inside the heat-insulating cover, an aluminum film for sealing the film is arranged inside the aluminum film groove, and a temperature detection board card is arranged on one side of the outside of the heat-insulating cover.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211472638.6A CN115806873A (en) | 2022-11-17 | 2022-11-17 | Digital PCR amplification equipment |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202211472638.6A CN115806873A (en) | 2022-11-17 | 2022-11-17 | Digital PCR amplification equipment |
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| CN115806873A true CN115806873A (en) | 2023-03-17 |
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| CN202211472638.6A Pending CN115806873A (en) | 2022-11-17 | 2022-11-17 | Digital PCR amplification equipment |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110195016A (en) * | 2019-07-17 | 2019-09-03 | 中国人民解放军军事科学院军事医学研究院 | Temperature control objective table and detection device |
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2022
- 2022-11-17 CN CN202211472638.6A patent/CN115806873A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110195016A (en) * | 2019-07-17 | 2019-09-03 | 中国人民解放军军事科学院军事医学研究院 | Temperature control objective table and detection device |
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