CN110195016A - Temperature control objective table and detection device - Google Patents
Temperature control objective table and detection device Download PDFInfo
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- CN110195016A CN110195016A CN201910645409.1A CN201910645409A CN110195016A CN 110195016 A CN110195016 A CN 110195016A CN 201910645409 A CN201910645409 A CN 201910645409A CN 110195016 A CN110195016 A CN 110195016A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
- B01L3/50853—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates with covers or lids
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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Abstract
This application provides a kind of temperature control objective table and detection devices, are related to the technical field of medicine detection；Temperature control objective table includes the objective table ontology equipped at least one sample position, and is arranged on objective table ontology and can open or cover the cover board of sample position, and detection hole corresponding with sample position is opened up on cover board；It further include the first temperature adjustment part and the second temperature adjustment part, the first temperature adjustment part is arranged on the cover board, and side of the objective table ontology far from cover board is arranged in the second temperature adjustment part；Wherein, for the chamber heating between cover board and objective table and cover board, the second temperature adjustment part is used to be the heating and cooling of objective table ontology the first temperature adjustment part；And then adjust and control the temperature of the sample on objective table.Temperature control objective table provided by the present application is applied in PCR detection device, testing result can be made more accurate, after handling by analysis, fitting obtains more accurate fluorescent amplification curve.
Description
Technical field
This application involves the technical fields of medicine detection, more particularly, to a kind of temperature control objective table and detection device.
Background technique
It is template that polymerase chain reaction (polymerase chain reaction, PCR), which is using section of DNA,
Under archaeal dna polymerase and nucleotides substrate participate in jointly, which is expanded to sufficient amount, to carry out structure and function point
Analysis；Clinically quick diagnosis bacterial infectious disease etc. has particularly important meaning to PCR detection method.
The factor for influencing PCR detection method is mainly denaturation temperature and time, and temperature is excessively high or high-temperature time is too long, can be right
Taq enzyme activity and dNTP (deoxyribonucleoside triphosphate, deoxy-ribonucleoside triphosphate) molecule cause
Damage；Wherein, Taq enzyme is isolated from thermus aquaticus Thermus Aquaticus (Taq) with thermal stability
Archaeal dna polymerase.
Sample in PCR detection is placed on objective table, and the regulation and control to the sample temperature on objective table is current
The main problem faced.
Summary of the invention
Based on this, this application provides a kind of temperature control objective tables, to adjust and control the temperature of the sample on objective table.
This application provides a kind of temperature control objective tables, the objective table ontology including being equipped at least one sample position, Yi Jishe
The cover board of the sample position is set on the objective table ontology and can open or cover, is opened up on the cover board and the sample
The corresponding detection hole in position；
It further include the first temperature adjustment part and the second temperature adjustment part, the first temperature adjustment part is arranged on the cover board, and described second
The side of the objective table ontology far from the cover board is arranged in temperature adjustment part；The first temperature adjustment part is used to be the cover board, with
And the chamber heating between the objective table ontology and the cover board, the second temperature adjustment part are used to be the objective table ontology liter
Cooling.
Preferably, the second temperature adjustment part is semiconductor chilling plate, and is arranged in the outer surface of the second temperature adjustment part
Radiating piece.
Preferably, seaming position between the cover board and the objective table ontology is arranged the first heat preservation member, and described the
One heat preservation member is used to keep the temperature the chamber between the cover board and the objective table ontology.
Preferably, the second transparent heat preservation member is arranged in covering at the detection hole of the cover board, and second heat preservation member is used
It is kept the temperature in the chamber between the cover board and the objective table ontology.
Preferably, the temperature sensor for further including controller and being arranged on the objective table ontology, the temperature sensing
Device is used to detect the temperature of the objective table ontology, the controller respectively with the temperature sensor, the first temperature adjustment part
It is electrically connected with the second temperature adjustment part.
Present invention also provides a kind of detection devices, are applied in PCR detection, improve the accuracy of detection.
This application provides a kind of detection devices, including pick-up lens, light source and temperature control objective table；
The top of the temperature control objective table is arranged in the pick-up lens, and the light source is located on the week of the pick-up lens
It encloses, the pick-up lens can be aligned with the detection hole, for acquiring the image of the sample position.
Preferably, the light source is made of multiple light emitting diodes, and multiple light emitting diodes are arranged in a ring.
Preferably, optical filter is arranged in the surface of the light source.
It preferably, further include first movement device, the top of the objective table is arranged in the first movement device, described
Pick-up lens is arranged on the first movement device, and the first movement device is able to drive the pick-up lens in first party
It is moved up to second party, or, the pick-up lens can be along the first movement device in a first direction and second party
It moves up.
Preferably, further include the second mobile device, the pick-up lens is set in second mobile device, described
Two mobile devices are able to drive the pick-up lens and move up in third party, or, the pick-up lens can be along described
Two mobile devices are moved up in third party.
This application provides a kind of temperature control objective table and detection device, temperature control objective table includes being equipped at least one sample position
Objective table ontology, and be arranged on objective table ontology and can open or cover the cover board of sample position, opened up on cover board with
The corresponding detection hole in sample position；It further include the first temperature adjustment part and the second temperature adjustment part, the first temperature adjustment part is arranged on the cover board, and second adjusts
Side of the objective table ontology far from cover board is arranged in warm part；First temperature adjustment part is used for as cover board and objective table ontology and cover board
Between chamber heating, the second temperature adjustment part be used for be the heating and cooling of objective table ontology；And then it adjusts and controls the sample on objective table
Temperature.Temperature control objective table provided by the present application is applied in PCR detection device, testing result can be made more accurate, is passed through
After crossing analysis processing, fitting obtains more accurate fluorescent amplification curve.
To enable the above objects, features, and advantages of the application to be clearer and more comprehensible, preferred embodiment is cited below particularly, and cooperate
Appended attached drawing, is described in detail below.
Detailed description of the invention
Technical solution in ord to more clearly illustrate embodiments of the present application, below will be to needed in the embodiment attached
Figure is briefly described, it should be understood that the following drawings illustrates only some embodiments of the application, therefore is not construed as pair
The restriction of range for those of ordinary skill in the art without creative efforts, can also be according to this
A little attached drawings obtain other relevant attached drawings.
Fig. 1 shows the first visual angle schematic diagram of temperature control objective table provided by the embodiment of the present application；
Fig. 2 shows the second visual angle schematic diagrames of temperature control objective table provided by the embodiment of the present application；
Fig. 3 shows the first visual angle schematic diagram (shell of the internal structure of detection device provided by the embodiment of the present application
It is not shown)；
Fig. 4 shows the second visual angle schematic diagram (shell of the internal structure of detection device provided by the embodiment of the present application
It is not shown)；
Fig. 5 shows the inside partial structural diagram of detection device provided by the embodiment of the present application, and (shell does not show
Out).
Appended drawing reference: 1- temperature control objective table；11- objective table ontology；111- sample position；12- cover board；121- detection hole；13-
Second temperature adjustment part；14- radiating piece；2- pick-up lens；3- light source；31- light source mounting rack；4- first movement device；5- second is moved
Dynamic device.
Specific embodiment
To keep the purposes, technical schemes and advantages of the embodiment of the present application clearer, below in conjunction with the embodiment of the present application
Middle attached drawing, the technical scheme in the embodiment of the application is clearly and completely described, it is clear that described embodiment is only
It is some embodiments of the present application, instead of all the embodiments.The application being usually described and illustrated herein in the accompanying drawings is real
The component for applying example can be arranged and be designed with a variety of different configurations.Therefore, below to the application's provided in the accompanying drawings
The detailed description of embodiment is not intended to limit claimed scope of the present application, but is merely representative of the selected reality of the application
Apply example.Based on embodiments herein, those skilled in the art institute obtained without making creative work
There are other embodiments, shall fall in the protection scope of this application.
In the description of the embodiment of the present application, it should be noted that term " on ", "lower", "left", "right", "vertical",
The orientation or positional relationship of the instructions such as "horizontal", "inner", "outside" is to be based on the orientation or positional relationship shown in the drawings, or be somebody's turn to do
Invention product using when the orientation or positional relationship usually put, be merely for convenience of description of the present invention and simplification of the description, without
It is that the device of indication or suggestion meaning or element must have a particular orientation, be constructed and operated in a specific orientation, therefore not
It can be interpreted as limitation of the present invention.In addition, term " first ", " second ", " third " etc. are only used for distinguishing description, and cannot manage
Solution is indication or suggestion relative importance.
In addition, the terms such as term "horizontal", "vertical" are not offered as requiring component abswolute level or pendency, but can be slightly
Low dip.It is not to indicate that the structure has been had to if "horizontal" only refers to that its direction is more horizontal with respect to for "vertical"
It is complete horizontal, but can be slightly tilted.
In the description of the embodiment of the present application, it is also necessary to which explanation is unless specifically defined or limited otherwise, term
" setting ", " installation ", " connection ", " connection " shall be understood in a broad sense, for example, it may be being fixedly connected, be also possible to detachably connect
It connects, or is integrally connected；It can be mechanical connection, be also possible to be electrically connected；It can be directly connected, intermediate matchmaker can also be passed through
Jie is indirectly connected, and can be the connection inside two elements.It for the ordinary skill in the art, can be with concrete condition
Understand the concrete meaning of above-mentioned term in the present invention.
Embodiment one:
As shown in Figure 1 and Figure 2, the embodiment of the present application provides a kind of temperature control objective table 1, including is equipped at least one sample
The objective table ontology 11 of position 111, and it is arranged on objective table ontology 11 and can open or cover the cover board of sample position 111
12, detection hole 121 corresponding with sample position 111 is opened up on cover board 12；
It should be noted that one, two, three, even more sample positions can be equipped on objective table ontology 11
111, in order to improve the detection efficiency of sample, objective table ontology 11 is equipped with multiple sample positions 111, as shown in Fig. 2, the application is real
The sample position 111 that four rows four column are uniformly provided on the objective table ontology 11 of example is applied, each sample position 111 is cylinder shape groove,
And then a sample is placed on each sample position 111, sample position 111 can also limit sample.
The detection hole 121 on sample position 111 and cover board 12 on objective table ontology 11 corresponds, and shape is identical, and covers
The size of detection hole 121 on plate 12 is not less than the size of sample position 111, when objective table ontology 11 and the fastening of cover board 12, inspection
Gaging hole 121 can be aligned setting with sample position 111, can also be set on sample position 111, no matter which kind of method be used, will
Guarantee that the cooperation between sample position 111 and detection hole 121 does not influence the tight fit between objective table ontology 11 and cover board 12；Lid
Plate 12 can be fastened on objective table ontology 11, and then a chamber, this chamber are formed between cover board 12 and objective table ontology 11
Room includes the space formed between each sample position 111 and each detection hole 121, also includes its other than sample position 111
The space formed between the region and cover board 12 of his objective table ontology 11；Cover board 12 in the embodiment of the present application can be not only load
Object playscript with stage directions body 11 is kept the temperature, and then the sample on objective table ontology 11 is made to keep a stationary temperature, can also limit objective table
Sample on sample position 111 on ontology 11 is mobile.
In turn, the seam in order to guarantee the heat insulation effect of objective table ontology 11, between cover board 12 and objective table ontology 11
The first heat preservation member is arranged in position, and the first heat preservation member is used to keep the temperature the chamber between cover board 12 and objective table ontology 11；In cover board
The second transparent heat preservation member is arranged in covering at 12 detection hole 121, and the second heat preservation member is used for cover board 12 and objective table ontology 11
Between chamber heat preservation.
As shown in Figure 1, do not fastened between the cover board 12 on temperature control objective table 1 and objective table ontology 11 and second heat preservation
Part is transparent, thus the first heat preservation member and the second heat preservation member are not shown in figure.
By the first heat preservation member and the second heat preservation member, the space of sealing is formed between objective table ontology 11 and cover board 12；This
Apply for that the first heat preservation member of embodiment uses insulating sponge, connecing between cover board 12 and objective table ontology 11 is arranged in insulating sponge
Position is stitched, for being kept the temperature to the chamber between cover board 12 and objective table ontology 11, and then the indoor sample of chamber is kept the temperature；Second protects
Warm part uses heat insulation glass, and heat insulation glass covers the detection hole 121 of cover board 12, between cover board 12 and objective table ontology 11
Chamber heat preservation, and then to the indoor sample of chamber keep the temperature；Wherein, it is observed in order to not influence the sample in detection hole 121, second
Heat preservation member must use transparent material.
It further, further include the first temperature adjustment part and the second temperature adjustment part 13, the first temperature adjustment part is arranged on cover board 12, and second
Side of the objective table ontology 11 far from cover board 12 is arranged in temperature adjustment part 13；First temperature adjustment part is used to be cover board 12 and objective table
Chamber heating between ontology 11 and cover board 12, the second temperature adjustment part 13 are used to be 11 heating and cooling of objective table ontology.
The distributed effect of first temperature adjustment part and the second temperature adjustment part 13 mainly includes two kinds of situations, and the setting of the first temperature adjustment part is being covered
On plate 12, for the chamber heating between cover board 12 and objective table ontology 11 and cover board 12, and then objective table ontology is prevented
Sample on 11 generates fog at high temperature, influences Imaging Study, the setting of the second temperature adjustment part 13 is in objective table ontology 11 far from lid
The side of plate 12, for being 11 heating and cooling of objective table ontology；It should be noted that heating member, frequently with heating film, cooling piece is normal
Using semiconductor chilling plate.
The first temperature adjustment part of the embodiment of the present application uses heating film, is covered on cover board 12 close to the one of objective table ontology 11
Side for the chamber heating between cover board 12 and objective table ontology 11 and cover board 12, and then prevents on objective table ontology 11
Sample generate fog at high temperature, influence Imaging Study, due to vision dead zone, be not shown in the figure；Second temperature adjustment part 13 uses
Side of the objective table ontology 11 far from cover board 12 is arranged in semiconductor chilling plate, for being 11 heating and cooling of objective table ontology；Pass through
Control to two temperature adjustment parts, the temperature of objective table ontology 11 are controlled in a reasonable range, in turn, adjust sample
Temperature makes it in a reasonable range.Generally in practical applications, the first temperature adjustment part is heated to 105 DEG C and remains unchanged,
Cooled down by the second temperature adjustment part 13 to objective table ontology 11, the adjusting of Lai Shixian temperature.
Further, when the second temperature adjustment part 13 is semiconductor chilling plate, it is arranged in the outer surface of the second temperature adjustment part 13
Radiating piece 14, radiating piece 14 are heat sink, and heat sink can radiate to the second temperature adjustment part 13 using modes such as air-cooled or liquid coolings, into
And the embodiment of the present application uses air-cooled form, and fan is arranged in the lower section of radiating piece 14, since radiating piece 14 wraps up fan
Inside, and then fan is not shown in the figure.
It further include that controller (not shown) and the temperature sensor being arranged on objective table ontology 11 (do not show in figure
Out), temperature sensor is used to detect the temperature of objective table ontology 11, controller respectively with temperature sensor, the first temperature adjustment part and
The electrical connection of second temperature adjustment part 13；Temperature sensor detects the temperature of objective table ontology 11, and temperature signal is passed to controller,
Controller controls the heating of the first temperature adjustment part or 13 heating and cooling of the second temperature adjustment part, to control the temperature of objective table ontology 11.
The temperature control objective table 1 that the embodiment of the present application proposes includes objective table ontology 11 and is arranged on objective table ontology 11
Cover board 12, for objective table ontology 11 for carrying the detection pipe that joined sample to be tested, objective table can be opened or be covered to cover board 12
Ontology 11, opened up on cover board 12 with the one-to-one detection hole 121 in sample position 111 on objective table ontology 11, cover board 12 is not
It can only be kept the temperature for objective table ontology 11, so that the sample on objective table ontology 11 is kept a stationary temperature, can also limit
Sample on sample position 111 on objective table ontology 11 is mobile；First heat preservation member and the second heat preservation member to objective table ontology 11 with
Chamber heat preservation between cover board 12, and then the indoor sample of chamber is kept the temperature；The first temperature adjustment part is covered in 12 upper surface of cover board, for lid
Plate 12 heats；Second temperature adjustment part 13 is set in the bottom surface of objective table ontology 11, is cooled down for objective table, and in the second temperature adjustment part 13
Heat sink is arranged in lower surface, and heat sink radiates to the second temperature adjustment part 13 by the way of air-cooled；By to the first temperature adjustment part and
The control of two temperature adjustment parts 13, the temperature of objective table ontology 11 are controlled in a reasonable range, in turn, adjust the temperature of sample
Degree, makes it in a reasonable range.
Embodiment two:
Temperature control objective table 1 in embodiment one is applied in PCR detection, polymerase chain reaction (polymerase
Chain reaction, PCR) it is to utilize section of DNA for template, in the case where archaeal dna polymerase and nucleotides substrate participate in jointly, by this
Segment DNA is expanded to sufficient amount, to carry out structure and function analysis；Clinically quick diagnosis is bacillary for PCR detection method
Infectious disease etc. has particularly important meaning；The factor for influencing PCR detection method is mainly denaturation temperature and time, temperature
Excessively high or high-temperature time is too long, can be to Taq enzyme activity and dNTP (deoxyribonucleoside triphosphate, deoxy-
Ribonucleoside triphosphate) molecule damages.In turn, as shown in fig. 1~fig. 5, the embodiment of the present application provides
A kind of detection device is applied in PCR detection, including pick-up lens 2, light source 3 and temperature control objective table 1；
The top of temperature control objective table 1 is arranged in pick-up lens 2, and light source 3 is located on around pick-up lens 2, pick-up lens 2
It can be aligned with detection hole 121, for acquiring the image of sample position 111.
Due to introducing a kind of fluorescent chemical in real-time fluorescence quantitative PCR reacts, with PCR react progress,
PCR product is constantly accumulative, and also equal proportion increases fluorescence signal intensity.It is every to collect a fluorescence intensity signals by a circulation,
We can monitor the variation of product amount by fluorescence intensity change in this way, to obtain a fluorescence intensity curves figure.Into
And when pick-up lens 2 is directed at the sample in detection hole 121, it needs to be located on the light source 3 around pick-up lens 2 and issues by force
Enough light is spent, sample is irradiated, excites the fluorescer wherein added.
As shown in figure 5, light source mounting rack 31 is arranged in the lens location in pick-up lens 2, light is set on light source mounting rack 31
Source 3, light source 3 are made of multiple light emitting diodes, and light emitting diode is arranged in a ring.
It should be noted that light emitting diode modulates luminous power by modulating electric current power in which can be convenient, due to
It needs to excite fluorescer, so the light of light source 3 needs by force, and then uses multiple light emitting diode close-packed arrays, and ring
Around pick-up lens 2.
Optical filter (not shown) is arranged in the surface of light source 3；Optical filter only allows the light that can excite fluorescer to pass through, this
The fluorescent image that sample generates is in bright colors, and susceptibility is higher, has certain benefit to Image Acquisition.
In addition to this, detection device further includes first movement device 4, and first movement device 4 is arranged in objective table ontology 11
Top, pick-up lens 2 be arranged on first movement device 4, first movement device 4 is able to drive pick-up lens 2 in first party
It is moved up to second party, or, pick-up lens 2 can move in the first direction and a second direction along first movement device 4
It is dynamic.
Further include the second mobile device 5, pick-up lens 2 is set in the second mobile device 5, and the second mobile device 5 can
Pick-up lens 2 is driven to move up in third party, or, pick-up lens 2 can be moved up along the second mobile device 5 in third direction
It is dynamic.
It should be noted that first direction is X-direction, second direction is the Y direction vertical with first direction,
In, it is parallel with the upper surface of objective table ontology 11 that first direction and second direction are formed by plane, third direction be with X-axis and
The vertical Z-direction of Y-axis, upper surface of the third direction perpendicular to objective table ontology 11.
Light source 3 and pick-up lens 2 are set in the top of cover board 12, and light source 3 and pick-up lens 2 are installed in the second movement
On device 5, the second mobile device 5 is mounted on first movement device 4, so that pick-up lens 2 and light source 3 can be realized X-axis side
To, the movement of Y direction and Z-direction, so that it is directed at pick-up lens 2 with each detection hole 121 on cover board 12 one by one, into
Row illumination excitation and Image Acquisition；The picture signal of acquisition is sent into processor by pick-up lens 2, is handled by software analysis
Afterwards, fitting obtains fluorescent amplification curve.
In turn, the move mode of pick-up lens 2 includes two kinds: the first is motionless for pick-up lens 2, first movement device 4
It is mobile with the second mobile device 5, and then pick-up lens 2 is driven to move to X-direction, Y direction and Z-direction；It is for second
First movement device 4 and the second mobile device 5 are motionless, and pick-up lens 2 is moved along first movement device 4 and the second mobile device 5
It is dynamic；No matter which kind of move mode is used, the effect finally realized is the same, and in this not go into detail.
Since temperature control objective table 1 has above-mentioned technical effect, so the detection device with the temperature control objective table 1 also has
There is identical technical effect, details are not described herein.
It should also be noted that, the embodiment of the present application is so that temperature control objective table 1 is applied in PCR detection as an example, but not only
It is confined to this field, details are not described herein for the embodiment of other field.
Compared with the existing technology, the application has the advantage that
This application provides a kind of temperature control objective table and detection device, temperature control objective table 1 can adjust objective table ontology 11
Temperature, and then adjust the temperature of sample, make the more accurate of sample detection；Multiple samples can be detected continuously, improve detection
Efficiency；Temperature control objective table 1 is applied in PCR detection device, after carrying out illumination excitation and Image Acquisition, can be fitted to obtain glimmering
Light intensity is of great significance to the diagnosis etc. on clinical medicine.
Finally, it should be noted that embodiment described above, the only specific embodiment of the application, to illustrate the application
Technical solution, rather than its limitations, the protection scope of the application is not limited thereto, although with reference to the foregoing embodiments to this Shen
It please be described in detail, those skilled in the art should understand that: anyone skilled in the art
Within the technical scope of the present application, it can still modify to technical solution documented by previous embodiment or can be light
It is readily conceivable that variation or equivalent replacement of some of the technical features；And these modifications, variation or replacement, do not make
The essence of corresponding technical solution is detached from the spirit and scope of the embodiment of the present application technical solution, should all cover the protection in the application
Within the scope of.Therefore, the protection scope of the application shall be subject to the protection scope of the claim.
Claims (10)
1. a kind of temperature control objective table, which is characterized in that the objective table ontology including being equipped at least one sample position, and setting exist
On the objective table ontology and the cover board of the sample position can be opened or cover, it is right with the sample position to open up on the cover board
The detection hole answered；
It further include the first temperature adjustment part and the second temperature adjustment part, the first temperature adjustment part is arranged on the cover board, second temperature adjustment
The side of the objective table ontology far from the cover board is arranged in part；The first temperature adjustment part is used to be the cover board, Yi Jisuo
The chamber heating between objective table ontology and the cover board is stated, the second temperature adjustment part is used to go up and down for the objective table ontology
Temperature.
2. temperature control objective table according to claim 1, which is characterized in that the second temperature adjustment part is semiconductor chilling plate,
And radiating piece is set in the outer surface of the second temperature adjustment part.
3. temperature control objective table according to claim 1, which is characterized in that between the cover board and the objective table ontology
Seaming position be arranged the first heat preservation member, first heat preservation member be used for the chamber between the cover board and the objective table ontology
Room heat preservation.
4. temperature control objective table according to claim 1, which is characterized in that covering setting is saturating at the detection hole of the cover board
The second bright heat preservation member, second heat preservation member are used to keep the temperature the chamber between the cover board and the objective table ontology.
5. temperature control objective table according to claim 1, which is characterized in that further include controller and setting in the objective table
Temperature sensor on ontology, the temperature sensor are used to detect the temperature of the objective table ontology, the controller difference
It is electrically connected with the temperature sensor, the first temperature adjustment part and the second temperature adjustment part.
6. a kind of detection device, which is characterized in that including pick-up lens, light source, and as described in any one of Claims 1 to 5
Temperature control objective table；
The top of the temperature control objective table is arranged in the pick-up lens, and the light source is located on around the pick-up lens,
The pick-up lens can be aligned with the detection hole, for acquiring the image of the sample position.
7. detection device according to claim 6, which is characterized in that the light source is made of multiple light emitting diodes, more
A light emitting diode is arranged in a ring.
8. detection device according to claim 6, which is characterized in that optical filter is arranged in the surface of the light source.
9. detection device according to claim 6, which is characterized in that further include first movement device, the first movement
The top of the objective table is arranged in device, and the pick-up lens is arranged on the first movement device, the first movement
Device is able to drive the pick-up lens and moves in the first direction and a second direction, or, the pick-up lens can be along institute
First movement device is stated to move in the first direction and a second direction.
10. detection device according to claim 9, which is characterized in that further include the second mobile device, moved described second
The pick-up lens is set on dynamic device, second mobile device is able to drive the pick-up lens and moves up in third direction
It is dynamic, or, the pick-up lens can be moved up along second mobile device in third party.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910645409.1A CN110195016A (en) | 2019-07-17 | 2019-07-17 | Temperature control objective table and detection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910645409.1A CN110195016A (en) | 2019-07-17 | 2019-07-17 | Temperature control objective table and detection device |
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| CN110195016A true CN110195016A (en) | 2019-09-03 |
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| CN201910645409.1A Pending CN110195016A (en) | 2019-07-17 | 2019-07-17 | Temperature control objective table and detection device |
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Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
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| TW200712432A (en) * | 2005-09-16 | 2007-04-01 | Hon Hai Prec Ind Co Ltd | Heating geometric measure equipment |
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