CN212223005U - Heating structure of PCR instrument - Google Patents
Heating structure of PCR instrument Download PDFInfo
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- CN212223005U CN212223005U CN202021805961.7U CN202021805961U CN212223005U CN 212223005 U CN212223005 U CN 212223005U CN 202021805961 U CN202021805961 U CN 202021805961U CN 212223005 U CN212223005 U CN 212223005U
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Abstract
The utility model relates to a heating structure of PCR appearance, it includes heating module, is equipped with the hot side that is used for heating micro-fluidic chip on the heating module, the hot side is equipped with two at least zones of heating, is equipped with thermal-insulated area between each zone of heating. The heating structure of this kind of PCR appearance has two at least heating regions, and different heating temperature can be set for respectively according to the polymerase chain reaction needs of the sample that awaits measuring in each heating region to avoid going up and down repeatedly to adjust the heating temperature and cause the problem that latency is long.
Description
Technical Field
The utility model relates to a PCR appearance, especially a heating structure of PCR appearance.
Background
Polymerase Chain Reaction (PCR) is a molecular biology technique for amplifying and amplifying specific DNA fragments, and can be regarded as special DNA replication in vitro, and the greatest feature of PCR is that a trace amount of DNA can be greatly increased.
In the PCR, DNA is denatured at a high temperature of 95 ℃ in vitro to become a single strand, a primer and the single strand are combined according to the principle of base complementary pairing at a low temperature (usually about 60 ℃), the temperature is adjusted to the optimal reaction temperature (about 72 ℃) of DNA polymerase, and the DNA polymerase synthesizes a complementary strand along the direction from phosphate to pentose. In the current polymerase chain reaction process, the temperature of a heating module of a sample to be detected is repeatedly changed, so that the sample to be detected sequentially carries out the following reactions: denaturation of the DNA to become single stranded; primer annealing to form denatured single-stranded DNA; and primer extension using thermostable DNA polymerase. This cycle is repeated until a sufficient number of copies is obtained for analysis. However, the waiting process for the transition between the temperature increase and the temperature decrease of the heating module is long, so that the time for the PCR is long.
Disclosure of Invention
An object of the utility model is to provide a rational in infrastructure, have the zone of heating more than two, through the specific heating temperature who sets for each zone of heating, avoid relapseing the lift adjustment heating temperature and cause the long problem of latency, can improve the heating structure of PCR appearance of polymerase chain reaction speed.
The purpose of the utility model is realized like this:
the utility model provides a heating structure of PCR appearance, includes the heating module, is equipped with the heating surface that is used for heating micro-fluidic chip on the heating module, its characterized in that: the heating surface is provided with at least two heating zones, and a heat insulation belt is arranged between each heating zone.
The purpose of the utility model can also adopt the following technical measures to solve:
more specifically, the heating module comprises at least two metal heat conducting blocks, an electric heating body is arranged at the bottom or inside of each metal heat conducting block, a heating zone is formed on the top surface of each metal heat conducting block, the metal heat conducting blocks are mutually separated, and the heat insulation belt is formed between the metal heat conducting blocks. The heat-insulating belt separates the metal heat-conducting blocks, and mutual temperature influence among all the metal heat-conducting blocks is reduced.
As a further scheme, the metal heat conducting block is a copper block, the electric heating body is a thick film heating body, and the thick film heating body is arranged at the bottom of the copper block. The copper block has good heat conduction effect and more accurate temperature control.
As a further scheme, the heating module further comprises a heat insulation frame, the heat insulation frame is provided with embedded holes corresponding to the metal heat conduction blocks respectively, the embedded holes are separated from each other, the metal heat conduction blocks are embedded in the embedded holes, and the top surfaces of the metal heat conduction blocks are exposed out of the top surface of the heat insulation frame, so that the top surfaces of the metal heat conduction blocks are in contact with the microfluidic chip, the heat loss is smaller, and the temperature control is more accurate.
As a further scheme, the number of the metal heat conduction blocks is two, the two metal heat conduction blocks are respectively a first metal heat conduction block and a second metal heat conduction block, and a first light hole is formed between the heat insulation frame and the first metal heat conduction block corresponding to the first metal heat conduction block and the second metal heat conduction block; the heating temperature of the first metal heat-conducting block is 55-75 ℃, and the heating temperature of the second metal heat-conducting block is 94-97 ℃. The heating temperature of the first and second metal heat-conducting blocks is only set according to the favorable temperature of the current PCR, but not limited to this temperature range.
As a further scheme, the heating module further comprises a heat insulation bottom cover, the bottom of the heat insulation frame is provided with a sinking groove corresponding to the heat insulation bottom cover, the sinking groove is communicated with the embedded hole, the heat insulation bottom cover is arranged in the sinking groove and connected with the metal heat conduction block, the heat insulation bottom cover is provided with a second light hole corresponding to the first light hole, and the heat insulation bottom cover is provided with a first threading hole corresponding to the metal heat conduction block. The heat insulation bottom cover effectively reduces downward radiation and heat transfer of the metal heat conducting block.
As a further scheme, the heating module sets up on the fixing base, and the fixing base top surface corresponds the heating module and is equipped with the constant head tank, and the inner wall of constant head tank is equipped with the location platform, the chip reference column sets up on the location platform, in the heating module embedding constant head tank, the thermal-insulated frame outer wall of heating module corresponds the location platform and is equipped with the location bayonet socket, and the location bayonet socket is pegged graft with the location platform.
As a further scheme, the fixing seat is arranged on the bottom plate, a pushing assembly is further arranged above the fixing seat and comprises an installation table and a driving device used for pushing the installation table, a nozzle is arranged on the bottom surface of the installation table and communicated with the tube body, more than two chip positioning columns are arranged on the fixing seat and used for being in inserting fit with positioning holes of the microfluidic chip, nozzle holes are formed in the microfluidic chip, and when the chip positioning columns are sleeved on the positioning holes of the microfluidic chip in a one-to-one corresponding mode, the nozzle holes are opposite to the nozzle. The micro-fluidic chip is internally provided with a micro-channel, and a sample to be detected can flow in the micro-channel in order by injecting certain pressure into the micro-channel, so that the sample to be detected passes through different heating areas, and then the polymerase chain reaction is carried out. The microfluidic chip belongs to the prior art, and the more specific structure thereof is not described here. In addition, the micro-fluidic chip and the chip positioning column are positioned, so that the positioning of the micro-fluidic chip, the fixed seat and the nozzle head of the push platform assembly is realized, and the push platform assembly is pressed downwards to be matched with the nozzle hole of the micro-fluidic chip in a sealing manner.
As a further scheme, a chip bearing platform is arranged between the fixed seat and the pushing platform assembly, and a second positioning hole is formed in the chip bearing platform corresponding to the chip positioning column; the chip bearing platform is provided with a sinking platform for placing the microfluidic chip, the sinking platform is provided with an opening corresponding to the heating module, the edge of the opening is provided with a supporting convex edge, and the second positioning hole is arranged on the supporting convex edge.
As a further scheme, the chip bearing platform is in transverse movable fit with the bottom plate. The chip bearing platform is moved out of the fixed seat, so that the microfluidic chip can be conveniently placed.
The utility model has the advantages as follows:
(1) the heating structure of the PCR instrument is provided with at least two heating zones, and each heating zone can be respectively set with different heating temperatures according to the requirement of the polymerase chain reaction of a sample to be detected, so that the problem of long waiting time caused by repeatedly lifting and adjusting the heating temperatures is avoided;
(2) heat insulation belts are arranged among all heating zones of the heating structure of the PCR instrument, so that the temperature influence among all the heating zones is reduced.
Drawings
Fig. 1 is an exploded schematic view of an embodiment of the present invention.
Fig. 2 is a schematic view of the connection structure of the heating module and the fixing base of the present invention.
Fig. 3 is another angle structure diagram of fig. 2.
Fig. 4 is the exploded structure diagram of the heating module and the fixing base of the present invention.
Fig. 5 is another angle structure diagram of fig. 4.
FIG. 6 is a schematic structural view of the extension state (to be loaded with the microfluidic chip) of the stage assembly of the PCR apparatus of the present invention.
FIG. 7 is a schematic structural view of the stage assembly of the PCR apparatus of the present invention in a retracted state (loading the microfluidic chip).
Fig. 8 is a schematic view of the exploded structure of a microfluidic chip used in conjunction with the present invention.
Fig. 9 is a schematic top view of fig. 8.
FIG. 10 is a schematic sectional view A-A of FIG. 9.
FIG. 11 is a schematic sectional view of the structure of FIG. 9B-B.
Fig. 12 is an enlarged view of the structure at F in fig. 11.
FIG. 13 is a schematic cross-sectional view of the structure of FIG. 9 taken at C-C.
Fig. 14 is an enlarged schematic view of G in fig. 13.
Fig. 15 is a schematic sectional view of fig. 9 taken along line D-D.
FIG. 16 is a schematic cross-sectional view of E-E of FIG. 9.
Fig. 17 is an exploded view of the middle support platform assembly of the present invention.
Fig. 18 is another angle structure diagram of fig. 17.
Fig. 19 is a schematic view of a three-dimensional structure of the middle push table assembly of the present invention.
Fig. 20 is an exploded view of the middle pushing platform assembly of the present invention.
Fig. 21 is another angle structure diagram of fig. 20.
Fig. 22 is a schematic top view of the structure of fig. 21.
FIG. 23 is a schematic cross-sectional view of the structure of FIG. 22 taken at H-H.
Fig. 24 is an enlarged schematic view of the structure at I in fig. 23.
Fig. 25 is a block diagram of a further embodiment of the present invention.
Detailed Description
The present invention will be further described with reference to the accompanying drawings and examples.
Referring to fig. 1 to 25, a heating structure of a PCR instrument includes a heating module 20, a heating surface for heating a microfluidic chip 5 is disposed on the heating module 20, the heating surface has two heating regions, and a heat insulation band is disposed between each heating region. The method comprises the following steps: the heating module 20 comprises two metal heat conduction blocks, namely a first metal heat conduction block 201 and a second metal heat conduction block 202; the heating module 20 further includes a heat insulation frame 204, the heat insulation frame 204 is provided with a first embedded hole 2011 and a second embedded hole 2021 corresponding to the first metal heat conduction block 201 and the second metal heat conduction block 202, the embedded holes are separated from each other (the separated part forms the heat insulation belt), the first metal heat conduction block 201 and the second metal heat conduction block 202 are embedded in the first embedded hole 2011 and the second embedded hole 2021, respectively, and top surfaces of the first metal heat conduction block 201 and the second metal heat conduction block 202 are exposed out of a top surface of the heat insulation frame 204.
The metal heat conducting block is a copper block, a thick film heating body is arranged at the bottom of the copper block, a heating area is formed on the top surface of each copper block, and the heat insulation frame 204 is made of bakelite.
The heat insulation frame 204 is provided with a first light hole 203 corresponding to a space between the first metal heat conduction block 201 and the second metal heat conduction block 202.
The heating module 20 further includes a heat insulation bottom cover 206, the bottom of the heat insulation frame 204 is provided with a sinking groove 207 corresponding to the heat insulation bottom cover 206, the sinking groove 207 is communicated with the embedding hole, the heat insulation bottom cover 206 is arranged in the sinking groove 207 and connected with the metal heat conduction block, the heat insulation bottom cover 206 is provided with a second light hole 2064 corresponding to the first light hole 203, and the heat insulation bottom cover 206 is provided with a first threading hole 2063 corresponding to the metal heat conduction block.
The fixing seat 6 is arranged on the bottom plate 40, the pushing assembly 10 is further arranged above the fixing seat 6, the pushing assembly 10 comprises an installation table 4 and a driving device used for pushing the installation table 4, a nozzle 143 is arranged on the bottom surface of the installation table 4, the nozzle 143 is communicated with the tube body 2, more than two chip positioning columns 63 are arranged on the fixing seat 6, the chip positioning columns 63 are used for being in inserting fit with the positioning holes 53 of the microfluidic chip 5, nozzle holes 56 are formed in the microfluidic chip 5, and when the positioning holes 53 on the microfluidic chip 5 are sleeved with the chip positioning columns 63 in a one-to-one correspondence mode, the nozzle holes 56 are opposite to the nozzle 143.
Four Ruhr connectors 1 are distributed on the mounting table 4 in a rectangular mode, each Ruhr connector 1 is connected with the pipe body 2, the lower end of each Ruhr connector 1 extends out of the bottom surface of the mounting table 4, the nozzle 143 is arranged at the lower end of each Ruhr connector 1, a through hole is formed in the nozzle 143, and the through hole is communicated with the pipe body 2.
The luer connector 1 comprises a male connector 11, a female connector 14 and a soft sleeve 13, wherein the center of the male connector 11 is provided with a plug tube hole 111 penetrating through two ends of the male connector, the female connector 14 is provided with a stepped hole 141, the stepped hole 141 is communicated with a through hole 146 of a nozzle 143, a soft sleeve limiting step 144 and a tube limiting step 145 which are distributed up and down are arranged in the stepped hole 141, and the tube limiting step 145 is positioned above the through hole 146; the soft sleeve 13 is arranged on the soft sleeve limiting step 144, and the tube body 2 penetrates through the insertion tube hole 111 and the soft sleeve 13 and then is inserted into the tube limiting step 145; the mounting table 4 is provided with a connector mounting hole 41 corresponding to the female connector 14, the female connector 14 is inserted into the connector mounting hole 41 and is fixedly connected with the mounting table 4, the lower end of the male connector 11 is inserted into the stepped hole 141 and is in threaded connection with the stepped hole 141, and the soft sleeve 13 is tightly pressed between the lower end of the male connector 11 and the soft sleeve limiting step 144.
The joint mounting hole 41 penetrates through the upper end and the lower end of the mounting table 4, the outer end of the female joint 14 is also provided with a cushion surface 142, the outer diameter of the cushion surface 142 is larger than that of the female joint 14, and the nozzle 143 protrudes out of the bottom surface of the cushion surface 142; the upper end of the female terminal 14 is inserted into the terminal mounting hole 41 from below to above and protrudes onto the top surface of the mounting table 4, and the upper end of the female terminal 14 is connected to the lock nut 12.
The periphery of the upper end of the male connector 11 is provided with anti-skid grains 112.
Four Ruhr joints 1 are distributed on the mounting table 4 in a rectangular mode, and each Ruhr joint 1 is connected with a pipe body 2.
The driving device comprises a cylinder 3, and the outer end of a piston rod 31 of the cylinder 3 is in transmission connection with the mounting table 4.
The outer end of the piston rod 31 is connected with a flange 32, and the flange 32 is connected with the top surface of the mounting table 4.
The base plate 40 is provided with a bearing platform assembly 30, the bearing platform assembly 30 is connected with the base plate 40 through a linear guide rail 8, a bearing platform driving mechanism 9 is arranged between the bearing platform assembly 30 and the base plate 40, and the bearing platform driving mechanism 9 is arranged on the base plate 40 and is in transmission connection with the bearing platform assembly 30. The bearing platform assembly 30 comprises a chip bearing platform 7, and an elastic part is arranged between the chip bearing platform 7 and the linear guide rail 8. The chip bearing table 7 is arranged between the fixed seat 6 and the pushing table assembly 10, and the chip bearing table 7 is provided with a second positioning hole 72 corresponding to the chip positioning column 63; the chip bearing platform 7 is provided with a sinking platform for placing the microfluidic chip 5, the sinking platform is provided with an opening 73 corresponding to the heating module 20, the edge of the opening 73 is provided with a supporting convex edge 71, and the second positioning hole 72 is arranged on the supporting convex edge 71.
The micro-fluidic chip 5 and the supporting flange 71 are respectively provided with three first positioning holes 53 and three second positioning holes 72, and the three first positioning holes 53 and the three second positioning holes 72 are mutually corresponding and are distributed in a triangular shape. An orientation protrusion 731 is arranged in the sinking platform, an orientation groove 54 is arranged on the micro-fluidic chip 5 corresponding to the orientation protrusion 731, and the orientation groove 54 is in positioning, inserting and matching with the orientation protrusion 731.
The fixing base 6 is provided with three chip positioning columns 63, and the three chip positioning columns 63 are in inserting fit with the first positioning hole 53 and the second positioning hole 72. The top end of the chip positioning column 63 is provided with a conical head 631 with a narrow top and a wide bottom. The surface of the conical head is provided with an inclined plane which has a certain guiding function.
The micro-fluidic chip 5 is provided with two liquid injection ports, one gas inlet and one liquid outlet, wherein the liquid injection ports, the gas inlet and the liquid outlet (56 marked in the reference figure 8) are sleeved with silica gel cups 51, the silica gel cups 51 are provided with nozzle holes 52, and the silica gel cups 51 are connected into a whole through silica gel gaskets 511.
The bearing platform assembly 30 further comprises an elastic supporting device, the elastic supporting device comprises a supporting rod 77, the elastic part and a fixing groove 79, the supporting rod 77 is connected to the bottom of the chip bearing platform 7, and first limiting flanges 771 are arranged on two sides of the bottom of the supporting rod 77; fixed slot 79 is connected with linear guide 8, the fixed slot 79 top surface is equipped with fluting 791, the opening inboard of fluting 791 is equipped with the spacing turn-ups 793 of second, 77 lower parts of the support rod insert in the fluting 791, and with fluting 791 about sliding fit, first spacing turn-ups 771 keeps off with the spacing turn-ups 793 of second mutually, the elastic component is equipped with a plurality ofly, and along 77 length direction straight line distributions of support rod, the elastic component sets up between the bottom surface of support rod 77 and the fluting 791 bottom surface of fixed slot 79.
The inner wall of the second limiting flange 793 of the fixing groove 79 is provided with an antifriction convex rib 792.
The elastic supporting devices are provided with a left set and a right set, and one elastic supporting device of each set is arranged at the bottom of the chip bearing platform 7 from left to right; the elastic member is a spring 78, a positioning cavity 772 is formed at the bottom of the support rod 77 corresponding to the spring 78, and the upper end of the spring 78 is inserted into the positioning cavity 772.
The bearing platform assembly 30 further comprises a side plate 75 and a panel 74, wherein the outer side of the fixing groove 79 is connected with the side plate 75, and the front end of the fixing groove 79 is connected with the panel 74; the top end of the side plate 75 is provided with an inward flange 751, the left and right sides of the chip platform 7 are provided with outward flanges 76, and the top surfaces of the outward flanges 76 are blocked with the bottom surfaces of the inward flanges 751.
The cap assembly 30 further includes an end cap 710 disposed at a rear end of the fixing groove 79.
The linear guide rail 8 comprises a fixed block 81, a guide roller 82 and a slide rail 83, the fixed block 81 is fixedly connected with the bottom plate 40, the guide roller 82 is horizontally arranged on the fixed block 81 in a rotating mode, an annular groove 821 is arranged on the periphery of the guide roller 82, a guide groove is formed in the bottom of the slide rail 83, a guide convex rib 831 matched with the annular groove 821 is arranged on the inner wall of the guide groove, and the slide rail 83 is connected with the fixed groove 79.
The bearing platform driving mechanism 9 is a screw rod driving mechanism, the screw rod driving mechanism comprises a driving motor 91, a nut 92, a screw rod 93, a guide seat 94 and a connecting lug 95, the guide seat 94 is arranged on the bottom plate 40, the top surface of the guide seat 94 is a plane, the bottom surface of the nut 92 is a plane and is in sliding fit with the top surface of the guide seat 94, the connecting lug 95 is connected between the nut 92 and the side plate 75 of the bearing platform assembly 30, baffle plates are arranged at the front end and the rear end of the guide seat 94, the screw rod 93 is in threaded connection with the nut 92 and is rotatably arranged between the two baffle plates, and the driving motor 91 is arranged outside one baffle plate and is in transmission connection with the screw rod 93.
The PCR instrument further comprises a fluorescence detection module, a liquid storage container and an air source, wherein the fluorescence detection module is used for determining the images of the droplet reaction units on the microfluidic chip 5.
The fixing seat 6 is arched, the fluorescence detection module is arranged at the bottom of the fixing seat 6, and the fluorescence detection module detects the microfluidic chip through the first light-transmitting hole, the second light-transmitting hole and the third light-transmitting hole. The fluorescence detection module comprises a filter group, a PMT (photomultiplier tube) and a laser, wherein the PMT (photomultiplier tube) is electrically connected with the control circuit through the acquisition board, and the laser is electrically connected with the control circuit.
The air source is an air pump disposed on the base plate 40. The air outlet of the air pump is respectively connected with the liquid storage container and the air pipe through the pressure controller, and the liquid storage container and the air pipe are respectively connected with the nozzle 143; the air pump and the pressure controller are respectively electrically connected with the control circuit.
And a dryer, a buffer gas cylinder and a first safety valve are sequentially arranged between the gas outlet of the gas pump and the pressure controller.
The opening pressure of the first safety valve is 0.5 Mpa.
The pressure controller is provided with an input port and three output channels, the input port is connected with the safety valve, the three output channels are respectively a first channel, a second channel and a third channel, the liquid storage container comprises a first liquid storage bottle, a second liquid storage bottle and a waste liquid bottle, and the first channel, the second channel and the third channel are respectively connected with the first liquid storage bottle, the second liquid storage bottle and a gas pipe; the four nozzles 143 of the push table assembly 10 are respectively communicated with the first liquid storage bottle, the second liquid storage bottle, the waste liquid bottle and the air pipe through the pipe body 2.
The air pump is communicated with the outside of the machine body through a filter.
The buffer gas cylinder is further connected with a pressure release valve, and the pressure release valve is electrically connected with the control circuit.
The air pump is a micro piston pump, and the air pressure output by an air outlet of the micro piston pump is greater than 0.5 Mpa.
The air cylinder 3 of the push table assembly 10 is connected with the buffer air cylinder through an electromagnetic steering valve and a second safety valve, and the electromagnetic steering valve is electrically connected with the control circuit.
The opening pressure of the second safety valve is 0.2 Mpa.
The control circuit is also electrically connected with a power supply, a display and control terminal and a data transmission port.
The working principle is as follows: the bearing platform assembly 30 extends forwards, the orientation groove 54 of the microfluidic chip 5 is aligned with the orientation protrusion 731 of the chip bearing platform 7 and then placed into the sinking platform of the chip bearing platform 7, the bearing platform assembly 30 moves backwards between the fixed seat 6 and the push platform assembly 10 under the control of the bearing platform driving mechanism 9, and the second positioning hole 72 of the chip bearing platform 7 is opposite to the center of the chip positioning column 63 of the fixed seat 6. When the mounting table 4 of the push table assembly 10 moves downwards, the microfluidic chip 5 and the chip bearing table 7 are pushed to move downwards at the same time, when the second positioning hole 72 of the chip bearing table 7 and the positioning hole 53 of the microfluidic chip 5 are in contact with the conical head 631 of the chip positioning column 63, the conical head 631 guides the second positioning hole 72, the positioning hole 53 and the chip positioning column 63 to be concentric, so that the bearing table assembly 30, the microfluidic chip 5, the push table assembly 10 and the heating module 20 are positioned completely, the nozzle 143 of the luer 1 of the push table assembly 10 is ensured to be inserted into the nozzle hole 52 of the microfluidic chip 5, the pad surface 142 of the luer 1 is in sealing fit with the silicone rubber cup 51 of the microfluidic chip 5, and operations such as liquid injection and gas supply can be performed at the moment without leakage.
Because the micro-fluidic chip 5 is internally provided with an S-shaped micro-fluidic channel, a test sample is injected into the channel; one part of the microfluidic channel is positioned on the top surface of the first metal heat-conducting block 201, and the other part of the microfluidic channel is positioned on the top surface of the second metal heat-conducting block 202; the first metal heat-conducting block 201 and the second metal heat-conducting block 202 are heated to required temperatures by electric heaters respectively, and the test sample repeatedly passes through the top surface of the first metal heat-conducting block 201 and the top surface of the second metal heat-conducting block 202 by controlling the flow of the test sample in the microfluidic channel, so that the test sample is repeatedly subjected to thermal cycle until a sufficient copy number for analysis is obtained.
When the micro-fluidic chip 5 is subjected to thermal cycle treatment, the micro-fluidic chip 5 is pressed and positioned on the heating module 20 by the push table assembly 10, so that on one hand, the micro-fluidic chip 5 is attached to the heating module 20, the heat loss is reduced, and more accurate heat transfer is obtained; on the other hand, the nozzle 143 of the push platform assembly 10 is positioned and matched with the nozzle hole 56 of the microfluidic chip 5, so that the tightness of the tube body 2 in the air and liquid infusion process is ensured.
Claims (10)
1. The utility model provides a heating structure of PCR appearance, includes heating module (20), is equipped with the heating surface that is used for heating micro-fluidic chip (5) on heating module (20), its characterized in that: the heating surface is provided with at least two heating zones, and a heat insulation belt is arranged between each heating zone.
2. The heating structure of the PCR instrument of claim 1, wherein: the heating module (20) comprises at least two metal heat conducting blocks, an electric heating body is arranged at the bottom or inside of each metal heat conducting block, a heating area is formed on the top surface of each metal heat conducting block, the metal heat conducting blocks are mutually separated, and the heat insulation belt is formed between each metal heat conducting block and each metal heat conducting block.
3. The heating structure of the PCR machine as set forth in claim 2, wherein: the metal heat conducting block is a copper block, the electric heating body is a thick film heating body, and the thick film heating body is arranged at the bottom of the copper block.
4. The heating structure of the PCR machine as set forth in claim 2, wherein: the heating module (20) further comprises a heat insulation frame (204), the heat insulation frame (204) is provided with embedded holes corresponding to the metal heat conduction blocks respectively, the embedded holes are separated from each other, the metal heat conduction blocks are embedded in the embedded holes, and the top surfaces of the metal heat conduction blocks are exposed out of the top surfaces of the heat insulation frame (204).
5. The heating structure of the PCR instrument as set forth in claim 4, wherein: the heat insulation frame (204) is provided with a first light hole (203) corresponding to the position between the first metal heat conduction block (201) and the second metal heat conduction block (202); the heating temperature of the first metal heat conduction block (201) is 55-75 ℃, and the heating temperature of the second metal heat conduction block (202) is 94-97 ℃.
6. The heating structure of the PCR instrument of claim 5, wherein: the heating module (20) further comprises a heat insulation bottom cover (206), a sinking groove (207) is formed in the bottom of the heat insulation frame (204) corresponding to the heat insulation bottom cover (206), the sinking groove (207) is communicated with the embedded hole, the heat insulation bottom cover (206) is arranged in the sinking groove (207) and connected with the metal heat conduction block, a second light hole (2064) is formed in the heat insulation bottom cover (206) corresponding to the first light hole (203), and a first threading hole (2063) is formed in the heat insulation bottom cover (206) corresponding to the metal heat conduction block.
7. The heating structure of the PCR instrument of claim 5, wherein: heating module (20) set up on fixing base (6), and fixing base (6) top surface corresponds heating module (20) and is equipped with constant head tank (61), in heating module (20) embedding constant head tank (61).
8. The heating structure of the PCR instrument of claim 7, wherein: the fixing seat (6) is arranged on the bottom plate (40), the pushing assembly (10) is further arranged above the fixing seat (6), the pushing assembly (10) comprises an installation table (4) and a driving device used for pushing the installation table (4), a nozzle head (143) is arranged on the bottom surface of the installation table (4), the nozzle head (143) is communicated with the tube body (2), more than two chip positioning columns (63) are arranged on the fixing seat (6), the chip positioning columns (63) are used for being in plug-in fit with the positioning holes (53) of the microfluidic chip (5), nozzle holes (56) are formed in the microfluidic chip (5), and when the chip positioning columns (63) are sleeved on the microfluidic chip (5) in a one-to-one correspondence mode through the positioning holes (53), the nozzle holes (56) are right opposite to the nozzle head (143).
9. The heating structure of the PCR instrument of claim 8, wherein: a chip bearing platform (7) is arranged between the fixed seat (6) and the push platform assembly (10), and a second positioning hole (72) is formed in the chip bearing platform (7) corresponding to the chip positioning column (63); the chip bearing platform (7) is provided with a sinking platform for placing the microfluidic chip (5), the sinking platform is provided with an opening (73) corresponding to the heating module (20), the edge of the opening (73) is provided with a supporting convex edge (71), and the second positioning hole (72) is arranged on the supporting convex edge (71).
10. The heating structure of the PCR instrument as set forth in claim 9, wherein: the chip bearing platform (7) is in transverse movable fit with the bottom plate (40).
Priority Applications (1)
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| CN202021805961.7U CN212223005U (en) | 2020-08-25 | 2020-08-25 | Heating structure of PCR instrument |
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| CN202021805961.7U CN212223005U (en) | 2020-08-25 | 2020-08-25 | Heating structure of PCR instrument |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111925931A (en) * | 2020-08-25 | 2020-11-13 | 墨卓生物科技(上海)有限公司 | Heating structure of PCR instrument and chip positioning heating method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111925931A (en) * | 2020-08-25 | 2020-11-13 | 墨卓生物科技(上海)有限公司 | Heating structure of PCR instrument and chip positioning heating method |
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