CN212669664U

CN212669664U - Chip positioning liquid injection structure of PCR instrument

Info

Publication number: CN212669664U
Application number: CN202020392274.0U
Authority: CN
Inventors: 裴颢
Original assignee: Mezhuo Biotechnology Zhejiang Co ltd
Current assignee: Mezhuo Biotechnology Zhejiang Co ltd
Priority date: 2020-03-24
Filing date: 2020-03-24
Publication date: 2021-03-09
Anticipated expiration: 2030-03-24

Abstract

The utility model relates to a chip location of PCR appearance annotates liquid structure, it includes the fixing base and pushes away a subassembly, pushes away the top that a subassembly is located the fixing base, pushes away a subassembly and includes the mount table and be used for promoting the drive arrangement of mount table, and the mount table bottom surface is equipped with the mouth head, mouth head and body intercommunication, be equipped with the chip reference column more than two on the fixing base, the chip reference column is used for pegging graft the cooperation with micro-fluidic chip's locating hole, is equipped with the mouth hole on the micro-fluidic chip, and when the chip reference column was entangled to the locating hole one-to-one on the micro-fluidic chip, the mouth hole. According to the chip positioning liquid injection structure of the PCR instrument, the microfluidic chip, the fixing seat and the nozzle head of the push platform assembly are positioned by positioning the microfluidic chip and the chip positioning column, the push platform assembly is pressed down to be in sealing fit with the nozzle hole of the microfluidic chip, and separable liquid injection under pressure is realized.

Description

Chip positioning liquid injection structure of PCR instrument

Technical Field

The utility model relates to a medical instrument, in particular to a chip positioning liquid injection structure of a PCR instrument.

Background

Polymerase Chain Reaction (PCR) is a molecular biology technique for amplifying and amplifying specific DNA fragments, and can be regarded as special DNA replication in vitro, and the greatest feature of PCR is that a trace amount of DNA can be greatly increased.

In the PCR, DNA is denatured at a high temperature of 95 ℃ in vitro to become a single strand, a primer and the single strand are combined according to the principle of base complementary pairing at a low temperature (usually about 60 ℃), the temperature is adjusted to the optimal reaction temperature (about 72 ℃) of DNA polymerase, and the DNA polymerase synthesizes a complementary strand along the direction from phosphate to pentose.

The basic principle of current digital PCR analysis is to distribute a sample into a large number of droplets, each of which contains one or more samples to be tested, then perform PCR amplification on the samples to be tested, and analyze the droplets after PCR amplification. In PCR, two-phase or three-phase temperature cycling (referred to as "thermal cycling") is repeated to sequentially perform the following reactions: denaturation of the DNA to become single stranded; primer annealing to form denatured single-stranded DNA; and primer extension using thermostable DNA polymerase. This cycle is repeated until a sufficient number of copies is obtained for analysis. At present, there is mainly a continuous flow type PCR method, which has a process of injecting liquid into a microfluidic chip and a process of heating the microfluidic chip, and in the cyclic heating, it is necessary to ensure the liquid in the microfluidic chip to flow, so that it is necessary to ensure the accurate positioning between the microfluidic chip and the liquid injection device and the heating stage, and at the same time, it is necessary to ensure the good contact between the microfluidic chip and the heating stage. However, the current technology has no effective positioning scheme, so that the microfluidic chip is easy to move in the liquid injection process, and the positioning is inaccurate. The efficiency of PCR is reduced, and the sample of PCR is easy to leak or be polluted.

Disclosure of Invention

An object of the utility model is to provide a simple structure is reasonable, ensure that micro-fluidic chip, mount table and annotate the chip location of the accurate PCR appearance of liquid push table subassembly three location and annotate liquid structure.

The purpose of the utility model is realized like this:

the utility model provides a liquid structure is annotated in chip location of PCR appearance, includes the fixing base and pushes away a subassembly, pushes away the top that a subassembly is located the fixing base, pushes away a subassembly and includes the mount table and be used for promoting the drive arrangement of mount table, and the mount table bottom surface is equipped with the mouth head, mouth head and body intercommunication, its characterized in that: the fixed seat is provided with more than two chip positioning columns which are used for being matched with the positioning holes of the microfluidic chip in an inserting mode, the microfluidic chip is provided with nozzle holes, and when the positioning holes on the microfluidic chip are sleeved on the chip positioning columns in a one-to-one corresponding mode, the nozzle holes are opposite to the nozzle heads.

The purpose of the utility model can also adopt the following technical measures to solve:

as a more specific scheme, a chip bearing platform is arranged between the fixed seat and the pushing platform assembly, and a second positioning hole is formed in the chip bearing platform corresponding to the chip positioning column.

As a further scheme, the chip bearing platform is movably arranged between the fixed seat and the push platform assembly up and down, the fixed seat is provided with a heating module, the chip bearing platform is provided with a sinking platform used for placing the microfluidic chip, the sinking platform is provided with an opening corresponding to the heating module, the edge of the opening is provided with a supporting convex edge, and the second positioning hole is arranged on the supporting convex edge.

As a further scheme, a directional protrusion is arranged in the sinking platform, a directional groove is arranged on the microfluidic chip corresponding to the directional protrusion, and the directional groove is in positioning, inserting and matching with the directional protrusion.

As a further scheme, the fixing base corresponds the heating module and is equipped with the constant head tank, and the inner wall of constant head tank is equipped with the location platform, the chip reference column sets up on the location platform, in the heating module embedding constant head tank, the heating module outer wall corresponds the location platform and is equipped with the location bayonet socket, and the location bayonet socket is pegged graft with the location platform, realizes heating module and fixing base location cooperation.

As a further scheme, the fixing seat is arranged on the bottom plate, and the chip bearing platform is transversely and movably matched with the bottom plate.

As a further scheme, the top ends of the chip positioning columns are conical or round-head-shaped. The conical surface is provided with an inclined surface, the round-head surface is provided with an arc surface, and the inclined surface and the arc surface have certain guiding functions.

As a further scheme, the number of the chip positioning columns is three, and the three chip positioning columns are distributed in a triangular shape.

As a further scheme, the push table assembly further comprises a luer connector, the lower end of the luer connector extends out of the bottom of the mounting table, the nozzle head is convexly arranged at the lower end of the cushion surface, and the tube body is connected with the luer connector.

As a further scheme, the driving device comprises an air cylinder, and the outer end of a piston rod of the air cylinder is in transmission connection with the mounting table.

The utility model has the advantages as follows:

(1) according to the chip positioning liquid injection structure of the PCR instrument, the microfluidic chip and the chip positioning column are positioned, so that the positioning of the microfluidic chip, the fixed seat and the nozzle of the push table assembly is realized, the push table assembly is pressed down to be in sealing fit with the nozzle hole of the microfluidic chip, and separable pressurized liquid injection is realized;

(2) the chip positioning liquid injection structure of the PCR instrument is provided with a chip bearing platform which moves up and down, a microfluidic chip is placed on the chip bearing platform, and when the microfluidic chip is pressed down by a pushing platform assembly, the microfluidic chip and the chip bearing platform act together to descend and are in positioning fit with a chip positioning column;

(3) the chip bearing platform of the chip positioning liquid injection structure of the PCR instrument can also move back and forth, and the microfluidic chip can be conveniently placed by moving the chip bearing platform out of the fixed seat.

Drawings

Fig. 1 is an exploded schematic view of an embodiment of the present invention.

Fig. 2 is a schematic structural diagram of another embodiment of the present invention.

Fig. 3 is a schematic structural view of another working state of fig. 2.

Fig. 4 is a schematic view of the decomposition structure of the microfluidic chip of the present invention.

Fig. 5 is a schematic top view of the structure of fig. 4.

FIG. 6 is a schematic sectional view A-A of FIG. 5.

Fig. 7 is an enlarged schematic view of the structure at C in fig. 6.

FIG. 8 is a schematic sectional view of the structure of FIG. 5B-B.

Fig. 9 is an enlarged schematic view of D in fig. 8.

Detailed Description

The present invention will be further described with reference to the accompanying drawings and examples.

Referring to fig. 1 to 9, a chip positioning liquid injection structure of a PCR instrument includes a fixed seat 6 and a push table assembly 10, the push table assembly 10 is located above the fixed seat 6, the push table assembly 10 includes an installation table 4 and a driving device for pushing the installation table 4, a nozzle 143 is disposed on a bottom surface of the installation table 4, the nozzle 143 is communicated with a tube body 2, the fixed seat 6 is provided with two or more chip positioning columns 63, the chip positioning columns 63 are used for being in insertion fit with positioning holes 53 of a microfluidic chip 5, the microfluidic chip 5 is provided with nozzle holes 52, and when the positioning holes 53 on the microfluidic chip 5 are sleeved with the chip positioning columns 63 in a one-to-one correspondence manner, the nozzle holes 52 are opposite to the nozzle 143.

A chip bearing platform 7 is arranged between the fixed seat 6 and the pushing platform assembly 10, and a second positioning hole 72 is arranged on the chip bearing platform 7 corresponding to the chip positioning column 63.

The chip bearing platform 7 is movably arranged between the fixed seat 6 and the push platform assembly 10 up and down, the heating module 20 is arranged on the fixed seat 6, the chip bearing platform 7 is provided with a sinking platform used for placing the microfluidic chip 5, the sinking platform is provided with an opening 73 corresponding to the heating module 20, the edge of the opening 73 is provided with a supporting convex edge 71, and the second positioning hole 72 is arranged on the supporting convex edge 71.

An orientation protrusion 731 is arranged in the sinking platform, an orientation groove 54 is arranged on the micro-fluidic chip 5 corresponding to the orientation protrusion 731, and the orientation groove 54 is in positioning, inserting and matching with the orientation protrusion 731.

The micro-fluidic chip 5 is provided with two liquid injection ports, one gas inlet and one liquid outlet, wherein the liquid injection ports, the gas inlet and the liquid outlet (56 marked in figure 4) are sleeved with silica gel cups 51, the silica gel cups 51 are provided with nozzle holes 52, and the silica gel cups 51 are connected into a whole through silica gel gaskets 511.

The fixing seat 6 is provided with a positioning groove 61 corresponding to the heating module 20, a positioning table 62 is arranged on the inner wall of the positioning groove 61, the chip positioning column 63 is arranged on the positioning table 62, the heating module 20 is embedded into the positioning groove 61, a positioning bayonet 201 is arranged on the outer wall of the heating module 20 corresponding to the positioning table 62, and the positioning bayonet 201 is connected with the positioning table 62 in an inserting mode.

The fixed seat 6 is arranged on the bottom plate 40, and the chip bearing platform 7 is transversely and movably matched with the bottom plate 40.

Be equipped with linear guide 8, cushion cap subassembly 30 and screw drive mechanism 9 on bottom plate 40, cushion cap subassembly 30 includes chip cushion cap 7, curb plate 75, panel 74 and elasticity hold up the device, elasticity holds up the device and includes fixed slot 79, bracing piece 77 and spring 78, be equipped with the inner chamber of type of falling T in the fixed slot 79, bracing piece 77 is the type of falling T, bracing piece 77 inserts in the inner chamber of fixed slot 79, the lower part both sides of bracing piece 77 keep off mutually with the upper portion both sides of inner chamber and spacing, spring 78 is equipped with a plurality ofly, and along bracing piece 77 length direction straight line distribution, spring 78 sets up between the bottom surface of bracing piece 77 and the inner chamber bottom surface of fixed slot 79. The elastic supporting devices are provided with a left set and a right set and are respectively arranged beside the left side and the right side of the fixed seat 6, and the elastic supporting devices are connected with the linear guide rail 8. The outer side of the fixing groove 79 is connected to the side plate 75, and the front end of the fixing groove 79 is connected to the panel 74. The top end of the side plate 75 is provided with an inner flange 751, the chip bearing platform 7 is connected with the support rod 77, the left side and the right side of the chip bearing platform 7 are provided with outer flanges 76, and the top surfaces of the outer flanges 76 are blocked with the bottom surfaces of the inner flanges 751. The screw transmission mechanism 9 comprises a driving motor 91, a nut 92, a screw rod 93, a guide seat 94 and a connecting lug 95, wherein the guide seat 94 is arranged on the bottom plate 40, the top surface of the guide seat 94 is a plane, the bottom surface of the nut 92 is a plane and is in sliding fit with the top surface of the guide seat 94, the connecting lug 95 is connected between the nut 92 and the side plate 75 of the bearing platform assembly 30, baffle plates are arranged at the front end and the rear end of the guide seat 94, the screw rod 93 is in threaded connection with the nut 92 and is rotatably arranged between the two baffle plates, and the driving motor 91 is arranged outside one baffle plate and is in transmission connection with the screw rod 93.

The top end of the chip positioning column 63 is provided with a conical head 631 with a narrow top and a wide bottom.

The number of the chip positioning columns 63 is three, and the three chip positioning columns 63 are distributed in a triangular shape.

The push bench assembly 10 further comprises a luer 1, the lower end of the luer 1 extends out of the bottom of the mounting table 4 and is provided with a cushion surface 142, the nozzle 143 is arranged at the lower end of the cushion surface 142 in a protruding manner, and the tube body 2 is connected with the luer 1. The medium transported by the tube 2 corresponding to each luer 1 may be liquid or gas.

The driving device comprises a cylinder 3, and the outer end of a piston rod 31 of the cylinder 3 is in transmission connection with the mounting table 4.

The working principle is as follows: the bearing platform assembly 30 extends forwards, the orientation groove 54 of the microfluidic chip 5 is aligned with the orientation protrusion 731 of the chip bearing platform 7 and then placed into the sinking platform of the chip bearing platform 7, the bearing platform assembly 30 moves backwards between the fixed seat 6 and the push platform assembly 10 under the control of the lead screw transmission mechanism 9, and the second positioning hole 72 of the chip bearing platform 7 is opposite to the center of the chip positioning column 63 of the fixed seat 6. When the mounting table 4 of the push table assembly 10 moves downwards, the microfluidic chip 5 and the chip bearing table 7 are pushed to move downwards at the same time, when the second positioning hole 72 of the chip bearing table 7 and the positioning hole 53 of the microfluidic chip 5 are in contact with the conical head 631 of the chip positioning column 63, the conical head 631 guides the second positioning hole 72, the positioning hole 53 and the chip positioning column 63 to be concentric, so that the bearing table assembly 30, the microfluidic chip 5, the push table assembly 10 and the heating module 20 are positioned completely, the nozzle 143 of the luer 1 of the push table assembly 10 is ensured to be inserted into the nozzle hole 52 of the microfluidic chip 5, the pad surface 142 of the luer 1 is in sealing fit with the silicone rubber cup 51 of the microfluidic chip 5, and operations such as liquid injection and gas supply can be performed at the moment without leakage.

Claims

1. The utility model provides a liquid structure is annotated in chip location of PCR appearance, includes fixing base (6) and pushes away a subassembly (10), pushes away a subassembly (10) and is located the top of fixing base (6), pushes away a subassembly (10) and includes mount table (4) and the drive arrangement who is used for promoting mount table (4), and mount table (4) bottom surface is equipped with tip (143), and tip (143) and body (2) intercommunication, its characterized in that: the fixing seat (6) is provided with more than two chip positioning columns (63), the chip positioning columns (63) are used for being in inserting fit with the positioning holes (53) of the microfluidic chip (5), the microfluidic chip (5) is provided with nozzle holes (52), and when the positioning holes (53) on the microfluidic chip (5) are sleeved on the chip positioning columns (63) in a one-to-one correspondence mode, the nozzle holes (52) are opposite to the nozzle heads (143).

2. The chip positioning liquid injection structure of the PCR instrument according to claim 1, wherein: a chip bearing platform (7) is arranged between the fixed seat (6) and the pushing platform assembly (10), and a second positioning hole (72) is formed in the chip bearing platform (7) corresponding to the chip positioning column (63).

3. The chip positioning liquid injection structure of the PCR instrument according to claim 2, wherein: the chip bearing platform (7) is movably arranged between the fixed seat (6) and the push platform assembly (10) from top to bottom, the fixed seat (6) is provided with the heating module (20), the chip bearing platform (7) is provided with a sinking platform used for placing the microfluidic chip (5), the sinking platform is provided with an opening (73) corresponding to the heating module (20), the edge of the opening (73) is provided with a supporting convex edge (71), and the second positioning hole (72) is arranged on the supporting convex edge (71).

4. The chip positioning liquid injection structure of the PCR instrument according to claim 3, wherein: an orientation protrusion (731) is arranged in the sinking platform, an orientation groove (54) is arranged on the micro-fluidic chip (5) corresponding to the orientation protrusion (731), and the orientation groove (54) is in positioning, inserting and matching with the orientation protrusion (731).

5. The chip positioning liquid injection structure of the PCR instrument according to claim 1, wherein: fixing base (6) are equipped with constant head tank (61) corresponding heating module (20), and the inner wall of constant head tank (61) is equipped with location platform (62), chip reference column (63) set up on location platform (62), in heating module (20) embedding constant head tank (61), heating module (20) outer wall corresponds location platform (62) and is equipped with location bayonet socket (201), and location bayonet socket (201) are pegged graft with location platform (62).

6. The chip positioning liquid injection structure of the PCR instrument according to claim 2, wherein: the fixing seat (6) is arranged on the bottom plate (40), and the chip bearing platform (7) is transversely and movably matched with the bottom plate (40).

7. The chip positioning liquid injection structure of the PCR instrument according to claim 1, wherein: the top end of the chip positioning column (63) is conical or round-head-shaped.

8. The chip positioning liquid injection structure of the PCR instrument according to claim 1, wherein: the number of the chip positioning columns (63) is three, and the three chip positioning columns (63) are distributed in a triangular shape.

9. The chip positioning liquid injection structure of the PCR instrument according to claim 1, wherein: the push bench component (10) further comprises a luer connector (1), the lower end of the luer connector (1) extends out of the bottom of the mounting table (4) and is provided with a pad surface (142), the nozzle head (143) is arranged at the lower end of the pad surface (142) in a protruding mode, and the tube body (2) is connected with the luer connector (1).

10. The chip positioning liquid injection structure of the PCR instrument according to claim 1, wherein: the driving device comprises a cylinder (3), and the outer end of a piston rod (31) of the cylinder (3) is in transmission connection with the mounting table (4).