CN212404076U - Micro-droplet generation chip - Google Patents
Micro-droplet generation chip Download PDFInfo
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- CN212404076U CN212404076U CN202020439968.5U CN202020439968U CN212404076U CN 212404076 U CN212404076 U CN 212404076U CN 202020439968 U CN202020439968 U CN 202020439968U CN 212404076 U CN212404076 U CN 212404076U
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Abstract
A micro-droplet generation chip comprises a substrate, wherein an operating equipment connecting cavity and a liquid storage cavity are arranged in the substrate, the operating equipment connecting cavity is communicated with the liquid storage cavity, and the operating equipment connecting cavity is positioned above the liquid storage cavity; the upper end of the operating equipment connecting cavity penetrates through the top of the base body, so that the operating equipment connecting cavity is communicated with the external space of the base body; the liquid outlet channel is communicated with the liquid storage cavity, so that the liquid storage cavity is communicated with the external space of the base body; the operating equipment connecting cavity is internally provided with an operating equipment connecting part which is used for limiting the separation of the operating equipment and the operating equipment connecting cavity. The micro-droplet generation chip can be loaded on operation equipment such as a pipettor, a pressurizing device and the like, so that the operation equipment can drive the micro-droplet generation chip to move, and the operation equipment can conveniently finish the automatic operation of generating micro-droplets.
Description
Technical Field
The utility model belongs to digital PCR field, concretely relates to little liquid drop generates chip for digital PCR.
Background
Polymerase Chain Reaction (PCR) is one of the mainstream nucleic acid detection technologies today. Since birth, the PCR technology is continuously developed toward high sensitivity, high accuracy, high detection throughput and full process automation. The PCR has undergone the first generation terminal point PCR, the second generation real-time quantitative PCR and the development to the third generation absolute quantitative digital PCR.
The Digital PCR (Digital PCR-dPCR) technology is different from the early PCR technology, and disperses a nucleic acid sample into thousands of micro-reaction units, so that the number of nucleic acid templates in each micro-reaction unit is less than or equal to 1, and the fluorescence signals of all the micro-reaction units are subjected to statistical analysis after the reaction is finished. The technology does not depend on a standard curve and a reference sample used in a real-time fluorescence quantitative PCR technology, really realizes the absolute quantification of the single-molecule nucleic acid, and has more excellent sensitivity, specificity and accuracy.
The digital PCR micro-reaction unit generating chip is mainly divided into an array type production chip and a liquid drop type generating chip.
In the production of a chip array, thousands of micro reaction chambers are prepared independently on a chip, and then a PCR reaction solution is dispensed into the micro reaction chambers to form micro reaction units.
The droplet generation chip generates tens of thousands to millions of water-in-oil droplets based on step emulsification, and uniformly divides the PCR reaction solution into independent micro-reaction units.
However, the existing droplet generation chip cannot be automatically matched with an operation device such as a pipettor and a pressurizing device to generate tens of thousands to millions of water-in-oil droplets; for example, the existing operation process is as follows:
firstly, loading a liquid transfer sucker on the head of a liquid transfer device, and adding an oil phase mixed solution into a liquid drop collecting consumable;
then, the droplet generation chip is manually moved to the droplet collection consumable, so that the liquid outlet channel (the liquid outlet channel is a micron-sized fine micro-channel) on the droplet generation chip is immersed in the oil phase mixed liquid contained in the droplet collection consumable. The existing liquid drop type generating chip comprises a base body, a liquid storage cavity with an opening at the top is arranged in the base body, a plurality of liquid outlet channels are arranged at the bottom of the base body and communicated with the liquid storage cavity, so that liquid in the liquid storage cavity can flow out of the liquid outlet channels.
Then, the pipette sucks an appropriate amount of the reaction solution by the pipette tip, and injects the reaction solution into the reservoir chamber from the injection port.
And finally, a pressurizing device extends into the liquid storage cavity and pressurizes the liquid storage cavity, so that the reaction liquid in the liquid storage cavity flows out from the liquid outlet runner, the water phase reaction liquid enters the oil phase mixed liquid and generates tens of thousands to millions of water-in-oil droplets based on the stepped emulsification principle, and the PCR reaction liquid is uniformly divided into independent micro reaction units. And then manually taking the droplet generation chip away from the droplet collection consumable.
According to the process, the existing liquid-moving device head cannot load the droplet-type generating chip, namely the existing liquid-moving device cannot drive the droplet-type generating chip to move, and the droplet-type generating chip can be placed at the droplet collecting consumable material only in a manual mode. Similarly, the droplet-forming chips cannot be loaded onto the head of the pressing device, and the droplet-forming chips cannot be moved to the next station by the pressing device. Resulting in low operation efficiency, high artificial fatigue strength, and possible contamination of the reaction solution due to manual intervention.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide a: in view of the above problems, the present invention provides a droplet generation chip, which can be loaded on an operation device such as a pipette and a pressurizing device, so that the operation device can drive the droplet generation chip to move, thereby facilitating the operation device to complete the automatic operation of generating droplets.
The utility model adopts the technical scheme as follows:
a micro-droplet generation chip comprises a substrate, wherein an operating equipment connecting cavity and a liquid storage cavity are arranged in the substrate, the operating equipment connecting cavity is communicated with the liquid storage cavity, and the operating equipment connecting cavity is positioned above the liquid storage cavity; the upper end of the operating equipment connecting cavity penetrates through the top of the base body, so that the operating equipment connecting cavity is communicated with the external space of the base body; the liquid outlet channel is communicated with the liquid storage cavity, so that the liquid storage cavity is communicated with the external space of the base body; the operating equipment connecting cavity is internally provided with an operating equipment connecting part which is used for limiting the separation of the operating equipment and the operating equipment connecting cavity.
Due to the structure, the operating equipment connecting part is arranged in the operating equipment connecting cavity, when the head part of operating equipment such as a liquid transfer device, a pressurizing device and the like is inserted into the operating equipment connecting cavity, the operating equipment connecting part can limit the head part of the liquid transfer device or the head part of the pressurizing device to be separated from the operating equipment connecting cavity, so that the chip can be loaded on the operating equipment such as the liquid transfer device, the pressurizing device and the like, and the operating equipment such as the liquid transfer device, the pressurizing device and the like drives the chip to move.
Further, the operating equipment connecting part is an annular bulge arranged on the inner wall of the operating equipment connecting cavity;
or the operating equipment connecting part is a plurality of bulges arranged on the inner wall of the operating equipment connecting cavity, and the bulges are arranged on the inner wall of the operating equipment connecting cavity at equal intervals and surround a circle on the same plane.
Due to the structure, when the head of the operation equipment such as the liquid transfer device, the pressurizing device and the like is inserted into the operation equipment connecting cavity, the annular bulge or the protrusion can clamp the head of the operation equipment such as the liquid transfer device, the pressurizing device and the like, and the operation equipment is limited to be separated from the operation equipment connecting cavity.
Furthermore, the convex surface of the annular bulge is a circular arc surface; or the convex surface of the bulge is a circular arc surface.
Due to the existence of the arc surface, the head of the operation equipment such as the liquid transfer device, the pressurizing device and the like can be conveniently inserted into the operation equipment connecting cavity, and the head of the operation equipment such as the liquid transfer device, the pressurizing device and the like can be conveniently pulled out of the operation equipment connecting cavity after a certain force is exerted.
Further, the diameter of operating equipment connecting cavity lower extreme and stock solution chamber upper end butt joint department equals, the diameter in stock solution chamber reduces from last to down gradually.
The diameter of the liquid storage cavity is gradually reduced from top to bottom, so that the continuous insertion of heads of operating equipment such as a liquid transfer device, a pressurizing device and the like can be limited, and meanwhile, when the head of the pressurizing device is in contact with the side wall of the liquid storage cavity, line sealing can be formed, so that the head of the pressurizing device can inject gas into the liquid storage cavity to press liquid in the liquid storage cavity out of the liquid outlet flow channel; meanwhile, the diameter of the liquid storage cavity is gradually reduced from top to bottom, the pressure at the bottom of the liquid storage cavity can be gradually increased, and when the liquid outlet flow channel is located at the bottom of the liquid storage cavity, liquid can be favorably extruded from the liquid outlet flow channel.
Furthermore, the liquid outlet channels are arranged at the bottom of the base body, each liquid outlet channel is located on the same plane, extends along the radial direction of the base body, penetrates through the side wall of the base body and then is communicated with the bottom of the liquid storage cavity, and the liquid outlet channels are arranged on the base body at equal intervals for a circle.
Further, the base body comprises a main body part and a liquid outlet flow channel cover; the operating equipment connecting cavity and the liquid storage cavity are arranged in the main body part, and the upper end of the operating equipment connecting cavity penetrates through the top of the main body part and the lower end of the liquid storage cavity penetrates through the bottom of the main body part; a plurality of liquid outlet flow channel grooves are formed in the lower end face of the main body part, extend along the radial direction of the main body part and penetrate through the side wall of the main body part; the liquid outlet channel cover is covered on the lower end surface of the main body part; the liquid outlet runner groove and the liquid outlet runner cover are matched with each other to form a liquid outlet runner.
The liquid outlet channel groove belongs to a micro channel, so that the processing is not easy, and the processing is convenient when the liquid outlet channel groove and the liquid outlet channel cover are matched with each other to form the liquid outlet channel.
Furthermore, the width of the liquid outlet channel is more than or equal to 4 times of the height of the liquid outlet channel, so that the stepped emulsification condition can be met, and the micro-droplets can be conveniently produced.
Furthermore, the base body is fixedly sleeved with a supporting plate, and the supporting plate and the base body are integrally formed or detachably connected.
Due to the structure, the supporting disc can cover the opening of the liquid drop collecting consumable, so that the substrate of the chip is supported, and the chip is prevented from shaking or being positioned inaccurately in the use process to influence the alignment of the head of the operating equipment.
Furthermore, a plurality of supporting disk bottom grooves are formed in the bottom surface of the supporting disk; the supporting disk kerve extends along the radial direction of the supporting disk, extends from the middle part of the supporting disk to the edge of the supporting disk, and the edge of the supporting disk is notched.
Because the existence of supporting disk kerve, can avoid supporting disk and the opening part of liquid drop collection consumptive material to lead to propping the dish and collecting the condition emergence that the opening part bonding of consumptive material is too tight and unable separation because of the viscous force effect of atmospheric pressure or liquid. And in the pressurizing process of the pressurizing device, excessive pressurizing gas is extruded out from the notch, so that the damage caused by excessive pressure in the liquid drop collecting consumable is avoided.
Furthermore, the supporting plate comprises a first base plate and a second base plate which are fixedly sleeved on the base body, the second base plate is arranged at the bottom of the first base plate, and the diameter of the first base plate is larger than that of the second base plate; the first base plate, the second base plate and the base body are integrally formed or detachably connected; a plurality of supporting disc bottom grooves are formed in the bottom surface of the supporting disc; the supporting disk bottom groove extends along the radial direction of the supporting disk, starts from the lower edge of the second base disk, extends to the edge of the first base disk after passing through the two sides of the second base disk and the bottom surface of the first base disk, and enables the edges of the second base disk and the first base disk to be provided with notches.
Because above-mentioned structure, under some circumstances, base plate one can cover the opening part at the liquid drop collection consumptive material, and base plate two can insert in the collection consumptive material, make base plate two sides and the contact of collection consumptive material inner wall, and then make the more stable installation of chip on the collection consumptive material. The supporting disk kerve can avoid the condition that the opening part of the supporting disk and the collecting consumable material are bonded too tightly and cannot be separated. And in the pressurizing process of the pressurizing device, excessive pressurizing gas is extruded out from the notch, so that the damage caused by excessive pressure in the liquid drop collecting consumable is avoided.
To sum up, owing to adopted above-mentioned technical scheme, the beneficial effects of the utility model are that:
the micro-droplet chip of the utility model is more suitable for the full process automation of the digital PCR reaction. Make in the digital PCR reaction flow liquid drop generate step and liquid drop collect step automation and be connected, because operating equipment connects the intracavity and is provided with the operating equipment connecting portion, make the utility model discloses a chip can load on operating equipment such as pipettor and pressure device, and operating equipment such as pipettor and pressure device of being convenient for drives the chip and removes. Simultaneously, current pipettor and pressure device are alternate segregation, make the module of automatic digital PCR system complicated like this, because the utility model discloses a chip structure exists, then can act as the pressure pipeline with the filling pipeline of current pipettor and use, reduces the module of automatic digital PCR system, when reduce cost, has promoted degree of automation greatly, has avoided manual operation, has reduced artifical fatigue strength.
Drawings
FIG. 1 is an overall structure of the present invention;
fig. 2 is a cross-sectional view of the present invention;
FIG. 3 is a view showing a starting position of a liquid outlet passage groove;
FIG. 4 is a view showing a lower end face of the main body;
FIGS. 5 and 6 show the connection state of the micro-droplet generation chip and the pipette in the droplet collection consumable.
Detailed Description
The present invention will be described in detail with reference to the accompanying drawings.
In order to make the objects, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
The utility model discloses a micro-droplet generation chip, which comprises a base body 1, wherein an operation device connecting cavity 3 and a liquid storage cavity 4 are arranged in the base body 1, the operation device connecting cavity 3 and the liquid storage cavity 4 are mutually communicated, and the operation device connecting cavity 3 is positioned above the liquid storage cavity 4; the upper end of the operating equipment connecting cavity 3 penetrates through the top of the base body 1, so that the operating equipment connecting cavity 3 is communicated with the external space of the base body 1; a plurality of liquid outlet channels 6 penetrating through the base body 1 are arranged on the base body 1, and the liquid outlet channels 6 are communicated with the liquid storage cavity 4, so that the liquid storage cavity 4 is communicated with the external space of the base body 1; an operating device connecting portion 2 is arranged in the operating device connecting cavity 3, and the operating device connecting portion 2 is used for limiting the separation of the pipettor 9 and the operating device connecting cavity 3.
The operating equipment connecting part 2 is an annular bulge arranged on the inner wall of the operating equipment connecting cavity 3; or the operating equipment connecting part 2 is a plurality of bulges arranged on the inner wall of the operating equipment connecting cavity 3, and the bulges are arranged on the inner wall of the operating equipment connecting cavity 3 at equal intervals and surround a circle on the same plane.
As shown in fig. 2, the convex surface of the annular protrusion is a circular arc surface; or the convex surface of the bulge is a circular arc surface.
As shown in fig. 2, the diameter of the butt joint of the lower end of the operating equipment connecting cavity 3 and the upper end of the liquid storage cavity 4 is equal, and the diameter of the liquid storage cavity 4 is gradually reduced from top to bottom.
The liquid outlet channels 6 are arranged at the bottom of the base body 1, each liquid outlet channel 6 is located on the same plane, extends along the radial direction of the base body 1, penetrates through the side wall of the base body 1 and then is communicated with the bottom of the liquid storage cavity 4, and the liquid outlet channels 6 are arranged on the base body 1 at equal intervals for a circle.
As shown in fig. 1, the base 1 includes a main body 101 and a liquid outlet channel cover 102; the operating equipment connecting cavity 3 and the liquid storage cavity 4 are arranged in the main body part 101, and the upper end of the operating equipment connecting cavity 3 penetrates through the top of the main body part 101 and the lower end of the liquid storage cavity 4 penetrates through the bottom of the main body part 101; a plurality of liquid outlet channel grooves 601 are formed in the lower end face of the main body part 101, and the liquid outlet channel grooves 601 extend along the radial direction of the main body part 101 and penetrate through the side wall of the main body part 101; the effluent channel cover 102 covers the lower end surface of the main body part 101; the liquid outlet runner groove 601 and the liquid outlet runner cover 102 are mutually matched to form the liquid outlet runner 6.
In the present invention, the outlet flow channel cover 102 is connected to the lower end surface of the main body 101 by bonding; bonding is a technology that two homogeneous or heterogeneous semiconductor materials with clean surfaces and flat atomic levels are subjected to surface cleaning and activation treatment, are directly combined under certain conditions, and are bonded into a whole through Van der Waals force, molecular force and even atomic force. Since the liquid outlet channel 6 is thin and cannot be realized by means of glue adhesion, the liquid outlet channel 6 can be blocked by glue, and the liquid outlet channel cover 102 is arranged on the lower end face of the main body part 101 in a bonding mode. Meanwhile, in order that the section of the liquid outlet flow passage 6 meets the step emulsification condition, the width of the liquid outlet flow passage 6 is more than or equal to 4 times of the height of the liquid outlet flow passage 6.
In the utility model, the length of the base body 1 is 15 mm-17 mm, the diameter of the base body is 6 mm-8 mm, and the thickness of the liquid outlet channel cover is 1 mm-2 mm; the width of the liquid outlet flow passage 6 is 100 um-150 um, and the height of the liquid outlet flow passage 6 is 25 um-37.5 um. The inner wall of the liquid outlet flow passage is subjected to hydrophobic treatment, and the contact angle of liquid drops is less than 120 degrees.
As shown in fig. 1 and 2, a support plate is fixedly sleeved on the base body 1, and the support plate is integrally formed with the base body 1 or detachably connected with the base body.
A plurality of supporting disk bottom grooves 5 are formed in the bottom surface of the supporting disk; the supporting disk bottom groove 5 extends along the radial direction of the supporting disk, extends from the middle of the supporting disk to the edge of the supporting disk, and enables the edge of the supporting disk to be notched.
As shown in fig. 1 and 2, the supporting disc comprises a first base disc 8 and a second base disc 7 which are fixedly sleeved on the base body 1, the second base disc 7 is arranged at the bottom of the first base disc 8, and the diameter of the first base disc 8 is larger than that of the second base disc 7; the first base plate 8, the second base plate 7 and the base body are integrally formed or detachably connected; a plurality of supporting disk bottom grooves 5 are formed in the bottom surface of the supporting disk; the supporting disk kerve 5 extends along the radial direction of the supporting disk, starts from the lower edge of the second base disk 7, extends to the edge of the first base disk 8 after passing through the side surface of the second base disk 7 and the bottom surface of the first base disk 8, and enables the edges of the second base disk 7 and the first base disk 8 to be notched.
As shown in fig. 5 and 6, the operation device in the figures is a pipette, and meanwhile, the chip is placed at the droplet collection consumable 10, and the liquid outlet channel 6 is immersed in the oil phase mixed liquid contained in the droplet collection consumable 10; the first base plate 8 covers the opening of the liquid drop collecting consumable 10 to support and position the substrate 1, and the second base plate 7 is arranged in the liquid drop collecting consumable 10, and in fig. 5, the side surface of the second base plate is not in contact with the inner wall of the liquid drop collecting consumable 10.
In fig. 5 and 6, pipettor 9 head includes two circular arc pieces, two circular arc pieces and the cooperation that operating equipment connecting portion 2 that has the arc surface can be fine, because arc piece and operating equipment connecting portion 2 all have the arc surface, because of under the circumstances of exerting a certain power, insert operating equipment that pipettor 9 head can be convenient connect the chamber 3 in, also can be convenient exert the effect down of certain power, extract in connecting chamber 3 with pipettor 9 head follow operating equipment, make things convenient for operating equipment to connect chamber 3 and quick connection and the separation of pipettor 9.
When the pipette 9 is in a connection state with the operation device connection cavity 3, as shown in fig. 5, the highest point of the arc block is in contact with the highest point of the arc surface of the operation device connection portion 2, or the highest point of the arc block is located slightly below the highest point of the arc surface of the operation device connection portion 2.
Because the existing pipettor cannot be connected with the existing chip in a sealing way, the chip can be pressurized only by a single pressurizing device; but the utility model discloses a chip can load the pipettor head, makes the filling pipeline of pipettor can act as the pressurization pipeline and use, reduces the module of automatic digital PCR system.
As shown in fig. 5 and 6, the arc block at the front of the head of the liquid transfer device 9 is in line contact with the inner wall of the liquid storage cavity 4, so that the liquid storage cavity 4 can be well sealed, and air leakage can be avoided in the pressurizing process. The highest point of the arc block is in contact with the highest point of the arc surface of the operating device connecting part 2, and the sealing function can be achieved.
Due to the existence of the bottom groove 5 of the support plate, the edge of the support plate is provided with a gap, so that the air pressure in the liquid drop collection consumable 10 is communicated with the external air pressure, and the chip cannot be separated from the liquid drop collection consumable 10 due to the pressure difference; and in the pressurizing process of the pressurizing device, excessive pressurizing gas is extruded out from the notch, so that the damage caused by excessive pressure in the liquid drop collecting consumable is avoided.
The utility model discloses a chip can be very convenient load on operating equipment such as current pipettor 9 and pressure device, make things convenient for digital PCR automation mechanized operation, reduced the manual operation step, saved the manpower. And simultaneously, the good sealing in the pressurized liquid outlet can be ensured.
The above description is only exemplary of the present invention and should not be taken as limiting the scope of the present invention, as any modifications, equivalents, improvements and the like made within the spirit and principles of the present invention are intended to be included within the scope of the present invention.
Claims (10)
1. A micro-droplet generation chip, comprising: the liquid storage device comprises a base body (1), wherein an operating equipment connecting cavity (3) and a liquid storage cavity (4) are arranged in the base body (1), the operating equipment connecting cavity (3) is communicated with the liquid storage cavity (4), and the operating equipment connecting cavity (3) is positioned above the liquid storage cavity (4); the upper end of the operating equipment connecting cavity (3) penetrates through the top of the base body (1) to enable the operating equipment connecting cavity (3) to be communicated with the external space of the base body (1); a plurality of liquid outlet flow channels (6) penetrating through the base body (1) are arranged on the base body (1), and the liquid outlet flow channels (6) are communicated with the liquid storage cavity (4) to ensure that the liquid storage cavity (4) is communicated with the external space of the base body (1); an operating equipment connecting part (2) is arranged in the operating equipment connecting cavity (3), and the operating equipment connecting part (2) is used for limiting the separation of the operating equipment and the operating equipment connecting cavity (3).
2. A micro-droplet generation chip according to claim 1, wherein: the operating equipment connecting part (2) is an annular bulge arranged on the inner wall of the operating equipment connecting cavity (3);
or the operating equipment connecting part (2) is a plurality of bulges arranged on the inner wall of the operating equipment connecting cavity (3), and the bulges are surrounded into a circle on the same plane and are arranged on the inner wall of the operating equipment connecting cavity (3) at equal intervals.
3. A micro-droplet generation chip according to claim 2, wherein: the convex surface of the annular bulge is an arc surface; or the convex surface of the bulge is a circular arc surface.
4. A micro-droplet generation chip according to claim 1, wherein: the diameter of operating equipment connection chamber (3) lower extreme and stock solution chamber (4) upper end butt joint department equals, the diameter of stock solution chamber (4) reduces from last to down gradually.
5. A micro-droplet generation chip according to claim 1, wherein: the liquid outlet flow channels (6) are arranged at the bottom of the base body (1), each liquid outlet flow channel (6) is located on the same plane, extends along the radial direction of the base body (1), penetrates through the side wall of the base body (1) and then is communicated with the bottom of the liquid storage cavity (4), and the liquid outlet flow channels (6) are arranged on the base body (1) at equal intervals for a circle.
6. A micro-droplet generating chip according to claim 1 or 5, wherein: the base body (1) comprises a main body part (101) and a liquid outlet flow channel cover (102); the operating equipment connecting cavity (3) and the liquid storage cavity (4) are arranged in the main body part (101), and the upper end of the operating equipment connecting cavity (3) penetrates through the top of the main body part (101) and the lower end of the liquid storage cavity (4) penetrates through the bottom of the main body part (101); a plurality of liquid outlet channel grooves (601) are formed in the lower end face of the main body part (101), and the liquid outlet channel grooves (601) extend along the radial direction of the main body part (101) and penetrate through the side wall of the main body part (101); the effluent runner cover (102) is covered on the lower end surface of the main body part (101); the liquid outlet runner groove (601) and the liquid outlet runner cover (102) are mutually matched to form a liquid outlet runner (6).
7. A micro-droplet generation chip according to claim 1, wherein: the width of the liquid outlet flow passage (6) is more than or equal to 4 times of the height of the liquid outlet flow passage (6).
8. A micro-droplet generation chip according to claim 1, wherein: the fixed cover is equipped with the supporting disk on base member (1), the supporting disk and base member (1) integrated into one piece or detachable are connected.
9. A micro-droplet generation chip according to claim 8, wherein: a plurality of supporting disc bottom grooves (5) are formed in the bottom surface of the supporting disc; the supporting disk bottom groove (5) extends along the radial direction of the supporting disk, extends from the middle of the supporting disk to the edge of the supporting disk, and enables the edge of the supporting disk to be notched.
10. A micro-droplet generating chip according to claim 8 or 9, wherein: the supporting disc comprises a first base disc (8) and a second base disc (7) which are fixedly sleeved on the base body (1), the second base disc (7) is arranged at the bottom of the first base disc (8), and the diameter of the first base disc (8) is larger than that of the second base disc (7); the first base plate (8), the second base plate (7) and the base body are integrally formed or detachably connected; and the lower edge of the second base plate (7) extends to the edge of the first base plate (8) after passing through the side surface of the second base plate (7) and the bottom surface of the first base plate (8), so that the edges of the second base plate (7) and the first base plate (8) are notched.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202020439968.5U CN212404076U (en) | 2020-03-30 | 2020-03-30 | Micro-droplet generation chip |
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| CN202020439968.5U CN212404076U (en) | 2020-03-30 | 2020-03-30 | Micro-droplet generation chip |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113755563A (en) * | 2021-10-20 | 2021-12-07 | 西安天隆科技有限公司 | Method and system for quantifying nucleic acid molecules by using micro-droplets |
| CN113801925A (en) * | 2021-10-20 | 2021-12-17 | 西安天隆科技有限公司 | Container for containing quantitative liquid drop PCR oil-in-water emulsion and use method thereof |
-
2020
- 2020-03-30 CN CN202020439968.5U patent/CN212404076U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113755563A (en) * | 2021-10-20 | 2021-12-07 | 西安天隆科技有限公司 | Method and system for quantifying nucleic acid molecules by using micro-droplets |
| CN113801925A (en) * | 2021-10-20 | 2021-12-17 | 西安天隆科技有限公司 | Container for containing quantitative liquid drop PCR oil-in-water emulsion and use method thereof |
| CN113801925B (en) * | 2021-10-20 | 2023-10-24 | 西安天隆科技有限公司 | Oil-in-water emulsion container for accommodating quantitative liquid drop PCR and application method thereof |
| CN113755563B (en) * | 2021-10-20 | 2023-11-28 | 西安天隆科技有限公司 | Method and quantification system for quantifying nucleic acid molecules by using micro-droplets |
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