CN212040529U

CN212040529U - Miniature water bath suitable for micro-reaction system

Info

Publication number: CN212040529U
Application number: CN202020618832.0U
Authority: CN
Inventors: 周琳; 梅芳; 王锦; 车志伟
Original assignee: Chengdu Zhongzhu Jianlian Gene Technology Co ltd
Current assignee: Chengdu Zhongzhu Jianlian Gene Technology Co ltd
Priority date: 2020-04-22
Filing date: 2020-04-22
Publication date: 2020-12-01
Anticipated expiration: 2030-04-22

Abstract

The utility model discloses a micro water bath device suitable for a micro reaction system, which comprises a box body and a cover body arranged at the top of the box body, wherein the bottom of the box body is provided with a water tank, a heater is arranged in or at the bottom of the water tank, the heater is connected with a control module, and the control module is connected with a temperature sensor arranged on the inner side wall of the box body; a transparent water level observation column communicated with the bottom of the water tank is arranged on one side of the tank body; the base plate is detachably mounted in the box body above the water tank, and a plurality of pipe holes are formed in the base plate. The utility model realizes water bath heating by arranging the water tank, the heater and the base plate (provided with the pipe hole) in the box body and heating the EP pipe on the base plate by heating water, and has smaller structure and portability; through setting up the transparent water level observation post with the water tank intercommunication, utilize the linker principle, realize the visual observation water tank water level, in time add the water in the water tank, avoid appearing the dry combustion method state.

Description

Miniature water bath suitable for micro-reaction system

Technical Field

The utility model belongs to the technical field of the water-bath heating, concretely relates to miniature water bath ware suitable for micro-reaction system.

Background

The isothermal amplification technology is a novel in vitro nucleic acid amplification technology developed after the PCR technology. Compared with the common PCR technology, the whole reaction process only needs a relatively constant temperature. Compared with the common PCR technology, the method is faster and simpler, gets rid of the dependence on excellent equipment, and shows good application prospect in clinical and on-site rapid diagnosis.

At present, the heating at 0-100 ℃ is basically water bath heating or metal bath heating, and the template DNA is usually contained in an EP tube, so that the heat conduction performance between metal and an EP material is poor, the heating in the EP tube is not uniform, and the metal bath heating is not adopted. Common water bath equipment is large-scale equipment, when micro-reaction system experiments such as extraction of nucleic acid are involved, a commonly used method is that reaction containers such as an EP (ethylene propylene) tube and the like are fixed by a conventional large-scale common water bath kettle through a foam plate, the heating speed is low, and due to the obstruction of the foam plate, the heat in the EP tube is uneven, the experiment efficiency and the constant temperature effect are influenced, and meanwhile, large energy waste is caused.

Disclosure of Invention

The utility model aims to provide a miniature, portable and be applicable to the water bath of micro-reaction system.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

a micro water bath device suitable for a micro reaction system comprises a box body and a cover body arranged at the top of the box body, wherein a water tank is arranged at the bottom of the box body, a heater is arranged in or at the bottom of the water tank, the heater is connected with a control module, and the control module is connected with a temperature sensor arranged on the inner side wall of the box body; a transparent water level observation column communicated with the bottom of the water tank is arranged on one side of the tank body; the base plate is detachably mounted in the box body above the water tank, and a plurality of pipe holes are formed in the base plate.

Furthermore, glass fiber heat-insulating layers are arranged in the side wall of the box body and the cover body.

Furthermore, the pipe holes on the substrate are the same in size, and the diameter of each pipe hole is 0.2ml of the diameter of the EP pipe.

Furthermore, the pore sizes of the pores on the substrate are different, and the pore diameter at least comprises the pipe diameters of 0.2ml, 0.5ml, 1.5ml and 2.0ml EP pipes.

Furthermore, the inner side wall of the box body is provided with a limiting table, and the substrate is placed on the limiting table.

Furthermore, a limiting column is arranged on the limiting table, and a limiting hole matched with the limiting column is formed in the substrate.

Furthermore, the control module is also connected with a button and a display, and the button and the display are arranged on the side surface of the box body of the transparent water level observation column.

Furthermore, a water level line is arranged on the transparent water level observation column.

The utility model discloses following beneficial effect has:

(1) the water tank, the heater and the substrate (provided with the pipe holes) are arranged in the box body, and the EP pipe on the substrate is heated by heating water, so that water bath heating is realized, and the water bath heating box is small in structure and convenient to carry;

(2) by arranging the transparent water level observation column communicated with the water tank and utilizing the principle of a communicating vessel, the water level of the water tank can be visually observed, water in the water tank is added in time, and the dry burning state is avoided;

(3) the glass fiber heat-insulating layers are arranged on the box body and the cover body, so that the heat exchange between the inside and the outside can be reduced, and the heat-insulating effect is achieved;

(4) the base plate with the small holes with different sizes is arranged, so that the water bath device can be suitable for EP pipes with different sizes, and the application range of the water bath device provided by the utility model is wider;

(5) the substrate can be detachably mounted through the limiting table and the limiting column, so that the substrate is easy to replace and stable to mount;

(6) through setting up button and display, the accessible button adjusts the temperature and shows the temperature through the display in real time.

Drawings

Fig. 1 is a schematic sectional view of the present invention.

Fig. 2 is a schematic structural diagram of the outside of the box body of the present invention.

Fig. 3 is a schematic structural diagram of a substrate according to the present invention.

Fig. 4 is a schematic structural diagram of another substrate according to the present invention.

The labels in the figure are: 100. a box body; 101. a water tank; 102. a limiting table; 103. a limiting column; 200. a cover body; 300. a substrate; 301. a tube hole; 302. a limiting hole; 400. a transparent water level observation column; 401. a water line; 500. a control module; 501. a heater (one of the two heaters is selected in the figure); 502. a display; 503. a button; 504. a temperature sensor.

Detailed Description

As shown in fig. 1, the micro water bath suitable for the micro reaction system provided in this embodiment is configured in a portable micro structure, and is suitable for the micro reaction system.

The miniature water bath device comprises a box body 100 and a cover body 200, wherein the box body 100 can be a rectangular body or a cylinder, the cover body 200 is arranged at the top of the box body 100 and is connected and covered by conventional technical means such as hasps and the like, so that the heat exchange between the inside and the outside of the box is reduced, and glass fiber heat-insulating layers are filled in the box body 100 and the cover body 200. The inner side of the box 100 is made of a smooth material such as stainless steel which is easy to clean, and the outer side can be made of metal or hard plastic.

As shown in fig. 1, the bottom of the box 100 is a water tank 101, a water level line 401 is arranged on the inner side wall of the water tank for reminding a tester of water adding scales, a heater 501 for heating water in the water tank 101 is arranged in or at the bottom of the water tank 101, the heater 501 is a conventional water heater 501 in the prior art, the heater 501 is connected with a control module 500 for controlling the on or off of the heater, the control module 500 is installed in a cavity between the inner side and the outer side of the box 100, heat dissipation holes are formed in the bottom of the outer side, the control module 500 is connected with a temperature sensor 504 for detecting the temperature in the box 100, the temperature sensor 504 adopts a temperature sensor 504 of a conventional technical means, and specifically, the temperature sensor 504 is installed on the inner side wall of the box 100 and.

The inner side of the box body 100 is further provided with a limiting table 102, the limiting table 102 is located above the water tank 101, the embodiment is specifically arranged above the highest water level line 401, a substrate 300 is placed on the limiting table 102, a plurality of pipe holes 301 are formed in the substrate 300, the substrates 300 at least comprise two types, one type is the substrate 300 with the same pore size, as shown in fig. 3, the pore size is 0.2ml of the diameter of an EP pipe, and since 0.2ml of EP pipes are commonly used, the substrate 300 can basically and simultaneously heat a plurality of 0.2ml of EP pipes in a water bath, and is particularly suitable for constant-temperature amplification reaction; still another is to open a plurality of base plates 300 with different aperture sizes, as shown in fig. 4, the aperture at least includes the tube diameter of the common EP tube, such as 0.2ml, 0.5ml, 1.5ml, 2.0ml EP tube, so as to be convenient for carrying out water bath heating on different EP tubes simultaneously, and improve the utilization rate of this embodiment. In order to fix the substrate 300, at least two limiting holes 302 are formed in the substrate 300, limiting columns 103 are correspondingly formed in the limiting table 102, the limiting columns 103 are matched with the limiting holes 302, the limiting holes 302 are circular, square or triangular, and the limiting columns 103 are correspondingly cylinders, cuboids or triangular prisms.

As shown in fig. 2, the outside of box 100 is provided with transparent water level observation post 400 of water tank 101 bottom intercommunication, the bottom and the water tank 101 bottom of transparent water level observation post 400 keep level and communicate, and the top is kept level or is higher than water tank 101 with the water tank 101 top, utilizes the management of linker to realize water level observation, lets the experimenter directly perceivedly can know the water level in the water tank 101, be equipped with water level line 401 on the transparent water level observation post 400, water level line 401 can be conventional digital scale mark, also can be highest water level line 401 and emergent water level line 401.

The outer side of the box body 100 is also provided with a display 502 and a button 503, the display 502 and the button 503 are electrically connected with the control module 500, the button 503 is used for adjusting the temperature of the water bath, and the display 502 is used for displaying the temperature of the water bath; the installation is preferably performed on the same side of the transparent water level observation column 400, which facilitates the observation of the tester.

The control module 500 is realized by a single chip microcomputer module with data receiving, data processing and driving modules in the prior art, and controls whether the heater 501 is heated or not through the acquired water bath temperature and the water bath temperature set by the button 503, so that the temperature difference is not large, and the test is ensured to be carried out.

When the device is used, the water tank 101 is filled with water after the cover is opened, the reaction temperature is set through the button 503, and after the temperature reaches the set temperature, the EP pipes added into the reaction system are sequentially inserted into the pipe holes 301, and the cover body 200 is covered. After the reaction is finished, the EP tube is taken out for the next experiment.

The above is only the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any modification and replacement based on the technical solution and the utility model should be covered in the protection scope of the present invention.

Claims

1. The utility model provides a miniature water bath ware suitable for micro-reaction system, includes box (100) and sets up lid (200) at box (100) top, its characterized in that: the water tank (101) is arranged at the bottom of the tank body (100), the heater (501) is arranged in the water tank (101) or at the bottom of the water tank (101), the heater (501) is connected with the control module (500), and the control module (500) is connected with the temperature sensor (504) arranged on the inner side wall of the tank body (100); a transparent water level observation column (400) communicated with the bottom of the water tank (101) is arranged on one side of the tank body (100); a base plate (300) is detachably mounted in the box body (100) above the water tank (101), and a plurality of pipe holes (301) are formed in the base plate (300).

2. The micro water bath applicable to a micro reaction system according to claim 1, wherein: glass fiber heat-insulating layers are arranged in the side wall of the box body (100) and the cover body (200).

3. The micro water bath applicable to a micro reaction system according to claim 1, wherein: the sizes of the pipe holes (301) on the substrate (300) are the same, and the diameter of the pipe hole is 0.2ml of the diameter of an EP pipe.

4. The micro water bath applicable to a micro reaction system according to claim 1, wherein: the pore diameters of the pore holes (301) on the substrate (300) are different, and the pore diameters at least comprise the pipe diameters of 0.2ml, 0.5ml, 1.5ml and 2.0ml EP pipes.

5. The micro water bath applicable to a micro reaction system according to claim 3 or 4, wherein: the inner side wall of the box body (100) is provided with a limiting table (102), and the substrate (300) is placed on the limiting table (102).

6. The micro water bath applicable to a micro reaction system according to claim 5, wherein: the limiting platform (102) is provided with a limiting column (103), and the substrate (300) is provided with a limiting hole (302) matched with the limiting column (103).

7. The micro water bath applicable to a micro reaction system according to claim 1, wherein: the control module (500) is also connected with a button (503) and a display (502), and the button (503) and the display (502) are arranged on the side surface of the box body (100) of the transparent water level observation column (400).

8. The micro water bath applicable to a micro reaction system according to claim 1, wherein: and a water level line (401) is arranged on the transparent water level observation column (400).