CN205035380U - Novel portable isothermal nucleic acid amplification reaction device - Google Patents
Novel portable isothermal nucleic acid amplification reaction device Download PDFInfo
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- CN205035380U CN205035380U CN201520371595.1U CN201520371595U CN205035380U CN 205035380 U CN205035380 U CN 205035380U CN 201520371595 U CN201520371595 U CN 201520371595U CN 205035380 U CN205035380 U CN 205035380U
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- nucleic acid
- acid amplification
- amplification reaction
- isothermal nucleic
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Abstract
The utility model relates to a novel portable isothermal nucleic acid amplification reaction device belongs to food security short -term test equipment field. For solving the problem that current nucleic acid amplification reaction device costs an arm and a leg, operates the complicacy, can't be applicable to on -the -spot short -term test, the utility model discloses only by including by box body, hot paste and sample cell triplex, the structure is all very simple with the operation, do not rely on the water bath, very portable and do not need special training just can master to the user, the while low price, low in production cost for the disposable device just need not to overhaul to maintain, is suitable for on -the -spot short -term test and monitoring in real time very much.
Description
Technical field
The utility model relates to a kind of novel portable isothermal nucleic acid amplification reaction unit, belongs to food safety rapid detecting equipment field.
Background technology
Nucleic acid amplification is one of most valuable instrument of life science, and in public health, biology, the related fields such as clinical medicine are widely used, and especially have unique using value in food safety field of fast detection.Round pcr and Real-time quantitative PCR in the past in decades by a large amount of for food safety rapid detection, highly sensitive and specificity comparatively traditional method improves a lot, but, PCR method must have accurate temperature cycling device, these devices are expensive and be only limitted to laboratory operation, require higher to operator in addition, thus make it cannot meet the needs detected on the spot.Therefore, need badly exploitation set up sensitive, fast detection technique realize efficient detection.
Isothermal amplification is the emerging external constant temperature nucleic acid amplification method of one in this year.Utilize strand displacement archaeal dna polymerase can complete nucleic acid amplification reaction within the isothermal condition short period of time.Owing to not needing the process such as the thermally denature of template, long-time temperature cycle, loaded down with trivial details gel electrophoresis, ultraviolet imagery observation, overcome regular-PCR technology (PCR) in the restriction of plant and instrument and complex operation and shortcoming, nucleic acid amplification can be completed when constant temperature, thus there is quick, special, easy, economic advantage, become the another Fast Detection Technique means after PCR.
At present, isothermal amplification has been widely used in life science every field at present, but thermostat often lacks portability, and this limits the application of this technology in food safety rapid detection on the spot to a certain extent.Patent 94212802.8 discloses a kind of dependence four sea water baths and carries out PCR, realizes the automatic circulation device of gene amplification in vitro, but complex operation, rely on water-bath and not there is portability, and price comparison is expensive.Patent 200910096030.6 provides a kind of loop-mediated isothermal amplification fast detecting reagent kit, but this device also depends on water bath with thermostatic control provides PCR institute heat requirement, lacks portability.Patent 201220378379.6 discloses a kind of loop-mediated isothermal amplification pipe, but this reaction tubes only provides PCR reaction environment, does not relate to the device providing PCR institute heat requirement.
Utility model content
The utility model provides a kind of novel portable isothermal nucleic acid amplification reaction unit, belongs to food safety rapid detecting equipment field.For the deficiencies in the prior art, the purpose of this utility model is to solve that existing nucleic acid amplification reaction device is expensive, complicated operation, cannot be applicable to the problem of field quick detection.
Composition of the present utility model: comprise by box body, hot paste and sample cell three part.The outward appearance of described box body is the square of diameter 15-20cm, as preferably, material is polyurethane foam material, it has wear-resisting, heatproof, sealing, the advantage such as good processability and degradable, high-efficiency environment friendly, can prevent the pollution in experimentation and calorific loss, lid is arranged at box body top, and box body surrounding has ventilation hole simultaneously.Described lid is fixed with thermometer digital display screen and skylight, wherein thermometer digital display screen built-in temperature sensor, can Real-time Collection temperature signal, the temperature in aobvious screen display device; The outward appearance in described skylight is the square of diameter 2-4cm, regulates the temperature in the next artificial control device of the draught area in skylight by push-and-pull.The circular hole of the outward appearance of described ventilation hole to be diameter be 1-3mm, the every side 10-20 of box body is evenly distributed on surrounding, for device provides necessary oxygen.The outward appearance of described hot paste is the rectangle of 7 × 9cm, include can with the polymkeric substance of air empty oxygen effect generation thermopositive reaction, including, but not limited to iron, gac, inorganic salt and other material, can be attached on box side wall, continue 12 hours Stable Release heats.The outward appearance of described sample cell is three layers of aluminium alloy pipe support being uniformly distributed jack, and every layer cross section is the square of 10-15cm, can ensure the homogeneous of whole nucleic acid amplification envrionment temperature.Every layer of sample cell is provided with the aperture of 4 diameter 4mm and the macropore of 9 diameter 7mm, for vertically placing the reaction tubes of different size.
Operation steps of the present utility model: when carrying out isothermal nucleic acid amplification reaction, first four hot pastes be attached at four medial surfaces of box body and open thermometer digital display screen, the ventilation hole of box body surrounding contributes to air flowing, provides the oxygen needed for hot paste reacting by heating.After the temperature of thermometer digital display screen display reaches the temperature needed for isothermal nucleic acid amplification reaction, experiment sample is put into isothermal nucleic acid amplification reaction tubes, be placed in sample cell.Start to react.When thermometer digital display screen display temperature higher than isothermal nucleic acid amplification reaction needed for temperature time, the temperature in the next artificial control device of the draught area in skylight is regulated by push-and-pull, until be reduced to the temperature needed for isothermal nucleic acid amplification reaction, be so more conducive to the carrying out of isothermal nucleic acid amplification reaction.After reaction proceeds to required time, sample is taken out from sample cell.
The scope of application of the present utility model: common isothermal duplication comprises loop-mediated isothermal amplification technique, strand displacement isothermal amplification technique, nicking restriction endonuclease mediated isothermal amplification, rolling circle amplification, rely on the amplification technique of nucleotide sequence, rely on helicase isothermal amplification technology, recombinase polymeric enzymatic amplification technology, rely on the amplification technique of nucleotide sequence, chimeric primers mediation nucleic acid isothermal amplification technology etc., temperature of reaction is within 37-65 DEG C, duration of the reaction is at 30-60min, the utility model all can meet above all common isothermal amplification techniques, design parameter is in table 1.
The common isothermal duplication thermometer of table 1
Feature of the present utility model: not only structure and operation all very simple, its superiority is more embodied in and does not rely on water-bath, concerning very portable user and do not need specialized training just can grasp; Low price simultaneously, production cost is low, and for single use device and without the need to repair and maintenance, the scene that is suitable for very much is detected real-time and monitors.
In order to have better understanding to above-mentioned and other aspect of the present utility model, preferred embodiment cited below particularly, and accompanying drawing is coordinated to elaborate.
Accompanying drawing explanation
Fig. 1 is stereographic map of the present utility model.
Nomenclature:
Box body---(1)
Hot paste---(2)
Sample cell---(3)
Lid---(4)
Ventilation hole---(5)
Thermometer digital display screen---(6)
Skylight---(7)
Jack---(8)
Embodiment
Below in conjunction with accompanying drawing, the utility model device is described in further detail.
This novel portable isothermal nucleic acid amplification reaction unit, is made up of box body (1), hot paste (2) and sample cell (3) three part.Lid (4) is arranged at box body top, and lid is fixed with thermometer digital display screen (6) and skylight (7), box body surrounding has ventilation hole (5).At tray interior, heat posted is attached on sidewall.Sample cell is three layers of aluminium alloy pipe support sample cell being uniformly distributed jack (8), vertically places the reaction tubes of different size.
The utility model can attach multiple hot paste (2) in the side of box body (1), when carrying out isothermal nucleic acid amplification reaction, first four hot pastes (2) are attached at four medial surfaces of box body (1), the ventilation hole (5) of box body surrounding contributes to air flowing, provides the oxygen needed for hot paste reacting by heating.Four hot pastes (2) attached inside 40 ventilation holes (5) of this novel portable isothermal nucleic acid amplification reaction unit box body surrounding and box body can provide the temperature of reaction of more than 65 DEG C and 65 DEG C, and maintain more than 5 hours.Experiment sample is put into isothermal nucleic acid amplification reaction tubes, is placed in sample cell (3) after reaching the temperature needed for isothermal nucleic acid amplification reaction by the temperature shown when thermometer digital display screen (6).Start to react.When thermometer digital display screen display temperature higher than isothermal nucleic acid amplification reaction needed for temperature time, the temperature in the next artificial control device of the draught area in skylight (7) is regulated, until be reduced to the temperature needed for isothermal nucleic acid amplification reaction by push-and-pull.So more be conducive to the carrying out of isothermal nucleic acid amplification reaction.
In the present embodiment, sample cell (3) has three layers, every layer have 12 circular socket holes (8), such sample cell just can be placed 36 isothermal nucleic acid amplification reaction tubess simultaneously and carry out amplified reaction, that is adopt the utility model to detect 36 cdna samples simultaneously, be conducive to improving detection efficiency.
Circular socket holes of the present utility model (8) can be designed to different diameters, can meet the isothermal nucleic acid amplification reaction tubes of different model and the needs of isothermal nucleic acid amplification reaction.
As preferably, sample cell (3) adopts aluminum alloy material, because aluminum alloy material has good thermal conductivity, when carrying out isothermal nucleic acid amplification reaction, the heat that inside box body, hot paste produces is delivered to isothermal nucleic acid amplification reaction tubes more easily by sample cell, avoids the loss that heat is unnecessary.
Finally it should be noted that, above embodiment is only in order to illustrate the technical solution of the utility model but not restriction technologies scheme, although applicant has been described in detail the utility model with reference to most preferred embodiment, those of ordinary skill in the art is to be understood that, those are modified to the technical solution of the utility model or equivalent replacement, and do not depart from aim and the scope of the technical program, all should be encompassed in this and use in the middle of novel right.
Claims (6)
1. a novel portable isothermal nucleic acid amplification reaction unit, is made up of box body (1), hot paste (2) and sample cell (3) three part: box body outward appearance is the square of diameter 15-20cm, and material is polyurethane foam material; Hot paste outward appearance is the rectangle of 7 × 9cm, include can with the polymkeric substance of air empty oxygen effect generation thermopositive reaction; Sample cell is aluminium alloy pipe support, can ensure the homogeneous of whole nucleic acid amplification envrionment temperature.
2. a kind of novel portable isothermal nucleic acid amplification reaction unit according to claim 1, is characterized in that: lid (4) is arranged at described box body (1) top, and box body surrounding has ventilation hole (5).
3. a kind of novel portable isothermal nucleic acid amplification reaction unit according to claim 2, is characterized in that: described lid (4) is fixed with thermometer digital display screen (6) and skylight (7); The circular hole of described ventilation hole (5) outward appearance to be diameter be 1-3mm, the every side 10-20 of box body is evenly distributed on surrounding, for device provides necessary oxygen.
4. a kind of novel portable isothermal nucleic acid amplification reaction unit according to claim 3, it is characterized in that: described thermometer digital display screen (6) built-in temperature sensor, can Real-time Collection temperature signal, the temperature in aobvious screen display device; Described skylight (7) outward appearance is the square of diameter 2-4cm, regulates the temperature in the next artificial control device of the draught area in skylight by push-and-pull.
5. a kind of novel portable isothermal nucleic acid amplification reaction unit according to claim 1, is characterized in that: described hot paste (2) can be attached on box side wall, continues 12 hours Stable Release heats.
6. a kind of novel portable isothermal nucleic acid amplification reaction unit according to claim 1, it is characterized in that: described sample cell (3) is three layers of aluminium alloy pipe support being uniformly distributed jack (8), every layer is provided with the aperture of 4 diameter 4mm and the macropore of 9 diameter 7mm, for vertically placing the reaction tubes of different size.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520371595.1U CN205035380U (en) | 2015-06-02 | 2015-06-02 | Novel portable isothermal nucleic acid amplification reaction device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520371595.1U CN205035380U (en) | 2015-06-02 | 2015-06-02 | Novel portable isothermal nucleic acid amplification reaction device |
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| Publication Number | Publication Date |
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| CN205035380U true CN205035380U (en) | 2016-02-17 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201520371595.1U Expired - Fee Related CN205035380U (en) | 2015-06-02 | 2015-06-02 | Novel portable isothermal nucleic acid amplification reaction device |
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| CN (1) | CN205035380U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106367338A (en) * | 2016-08-30 | 2017-02-01 | 北京化工大学 | CPCR (convective polymerase chain reaction) amplification detection device based on chemical heating |
-
2015
- 2015-06-02 CN CN201520371595.1U patent/CN205035380U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106367338A (en) * | 2016-08-30 | 2017-02-01 | 北京化工大学 | CPCR (convective polymerase chain reaction) amplification detection device based on chemical heating |
| CN106367338B (en) * | 2016-08-30 | 2019-03-29 | 北京化工大学 | A kind of CPCR augmentation detection device based on chemical heat |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160217 Termination date: 20210602 |