CN203616670U - PCR instrument temperature control system - Google Patents
PCR instrument temperature control system Download PDFInfo
- Publication number
- CN203616670U CN203616670U CN201320713067.0U CN201320713067U CN203616670U CN 203616670 U CN203616670 U CN 203616670U CN 201320713067 U CN201320713067 U CN 201320713067U CN 203616670 U CN203616670 U CN 203616670U
- Authority
- CN
- China
- Prior art keywords
- temperature
- circuit
- temperature control
- temperature sensor
- control system
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The utility model discloses a PCR instrument temperature control system. The system comprises a PC, a main control chip, a Flash memory, an EPROM memory, a first temperature sensor, a second temperature sensor, a third temperature sensor, a signal isolation circuit, an amplification filter circuit, an A/D conversion circuit, a sample pedestal, reaction tanks arranged on the sample pedestal, a heat cover arranged right above the reaction tank, a first temperature control circuit used for controlling the temperature of the pedestal, a second temperature control circuit used for controlling a temperature of a side wall and a heating circuit used for heating the heat cover. By using the system of the utility model, the temperatures of the pedestal and the side wall of a sample base can be controlled so that uniformity of temperature distribution of the sample base is guaranteed; the temperature of each reaction tank of the sample base and temperature maintenance time are controlled accurately; problems that precision of an existing temperature control system is not high and efficiency of DNA fragment amplification is low and so on are effectively solved.
Description
Technical field
The utility model relates to automatic control technology field, especially relates to a kind of PCR instrument temperature control system.
Background technology
The life science that develops into of Life Science Instruments provides effective tool and strong research means.The further investigation that its research from cellular level is developed by leaps and bounds on molecular level.Leave the support of these modernization Life Science Instruments, many great research projects and engineering all will be had difficulty in taking a step.PCR instrument be a kind ofly widely used, very important Life Science Instruments.PCR (polymerase chain reaction, PCR) is a kind of Enzymology method of external rapid amplifying DNA fragment specific, and the basic process of its PCR reaction is divided into three steps.The first step, DNA sex change (94 ℃), double-stranded DNA template is hydrogen bond rupture under heat effect, forms single stranded DNA; Second step, annealing (55 ℃), system temperature reduces, and primer is combined with DNA profiling, forms local double-stranded; The 3rd step, extends (72 ℃), and under the effect of Taq enzyme, 5 ends take dNTP as raw material from primer extend to 3 ends, synthesizes the DNA chain with template complementation.PCR instrument reaches different temperatures by Quality control exactly, and the DNA fragmentation being amplified is carried out to sex change, annealing and polymerization processing, to reach the object that the amount of DNA fragmentation is increased at double.Therefore, the time of temperature controlled precision, especially each temperature value is controlled, and directly affects the efficiency of DNA fragmentation amplification.
Existing PCR instrument all only carries out temperature sampling and control to sample base bottom conventionally, do not consider that the sidewall of sample base and the convection heat transfer of peripheral air are on the inhomogeneity impact of base-plate temp, thereby do not reach higher temperature-controlled precision, affected the efficiency of DNA fragmentation amplification.
Utility model content
Technical problem to be solved in the utility model is for above-mentioned deficiency of the prior art, one PCR instrument temperature control system is fast, accurately provided, the work simultaneously temperature of the base to sample base and sidewall is controlled, efficiently solve existing temperature control system precision not high, the problem such as the efficiency of DNA fragmentation amplification is low.
For solving the problems of the technologies described above, the technical solution adopted in the utility model is: a kind of PCR instrument temperature control system, it is characterized in that: comprise PC, main control chip, Flash storer, eprom memory, the signal isolation circuit sequentially connecting, filtering and amplifying circuit and A/D change-over circuit, the sample base being formed by base and sidewall, be arranged on the reactive tank for holding test tubes on described sample base, directly over being arranged on described reactive tank, form the heat lid of condensate water for maintaining described test tube head temperature at described test tube top to prevent test solution evaporation, for detecting in real time the first temperature sensor of the reactive tank temperature that is distributed in base center section, for detecting in real time the second temperature sensor near the reactive tank temperature of sidewall, for detecting in real time the three-temperature sensor of heat lid temperature, the first temperature control circuit that the temperature of base is controlled, the second temperature control circuit that the temperature of oppose side wall is controlled and the heater circuit that heat lid is heated, described the first temperature control circuit includes with the second temperature control circuit the photoelectrical coupler, D/A change-over circuit, power amplified drive circuit and the semiconductor chilling plate that are connected successively, the quantity of described semiconductor chilling plate is multiple, and multiple described semiconductor chilling plate is mounted on respectively the bottom surface of base and the surrounding of sidewall, described the first temperature sensor, the second temperature sensor and three-temperature sensor all join with signal isolation circuit, described A/D change-over circuit, the first temperature control circuit, the second temperature control circuit, heater circuit, Flash storer and eprom memory all join with main control chip, and described main control chip is connected by interface circuit with PC.
Above-mentioned a kind of PCR instrument temperature control system, is characterized in that: also comprise the LCD display of joining with main control chip.
Above-mentioned a kind of PCR instrument temperature control system, is characterized in that: described main control chip is ARM microprocessor.
Above-mentioned a kind of PCR instrument temperature control system, is characterized in that: described the first temperature sensor, the second temperature sensor and three-temperature sensor are platinum resistance thermometer sensor, PT1000.
Above-mentioned a kind of PCR instrument temperature control system, is characterized in that: described interface circuit is USB3.0 interface.
The utility model compared with prior art has the following advantages: simple in structure, easy to use, input cost is low; The work simultaneously temperature of the base to sample base and sidewall is controlled, guarantee the homogeneity of sample base Temperature Distribution, the temperature of each reactive tank and the time of temperature maintenance of sample base are controlled accurately, efficiently solve existing temperature control system precision not high, the problem such as the efficiency of DNA fragmentation amplification is low.
Below by drawings and Examples, the technical solution of the utility model is described in further detail.
Accompanying drawing explanation
Fig. 1 is schematic block circuit diagram of the present utility model.
Description of reference numerals:
1-PC; 2-main control chip; 3-Flash storer;
4-eprom memory; 5-signal isolation circuit; 6-filtering and amplifying circuit;
7-A/D change-over circuit; 8-base; 9-sidewall;
10-heat lid; The 11-the first temperature sensor; The 12-the second temperature sensor;
The 13-the three-temperature sensor; The 14-the first temperature control circuit; The 15-the second temperature control circuit;
16-heater circuit; 17-interface circuit; 18-LCD display.
Embodiment
As shown in Figure 1, the utility model comprises PC 1, main control chip 2, Flash storer 3, eprom memory 4, the signal isolation circuit 5 sequentially connecting, filtering and amplifying circuit 6 and A/D change-over circuit 7, the sample base being formed by base 8 and sidewall 9, be arranged on the reactive tank for holding test tubes on described sample base, directly over being arranged on described reactive tank, form the heat lid 10 of condensate water for maintaining described test tube head temperature at described test tube top to prevent test solution evaporation, for detecting in real time the first temperature sensor 11 of the reactive tank temperature that is distributed in base 8 center sections, for detecting in real time the second temperature sensor 12 near the reactive tank temperature of sidewall 9, for detecting in real time the three-temperature sensor 13 of heat lid 10 temperature, the first temperature control circuit 14 that the temperature of base 8 is controlled, the second temperature control circuit 15 that the temperature of oppose side wall 9 is controlled and the heater circuit 16 that heat lid 10 is heated, described the first temperature control circuit 14 includes with the second temperature control circuit 15 photoelectrical coupler, D/A change-over circuit, power amplified drive circuit and the semiconductor chilling plate that are connected successively, the quantity of described semiconductor chilling plate is multiple, and multiple described semiconductor chilling plate is mounted on respectively the bottom surface of base 8 and the surrounding of sidewall 9, described the first temperature sensor 11, the second temperature sensor 12 and three-temperature sensor 13 all join with signal isolation circuit 5, described A/D change-over circuit 7, the first temperature control circuit 14, the second temperature control circuit 15, heater circuit 16, Flash storer 3 and eprom memory 4 all join with main control chip 2, and described main control chip 2 is connected by interface circuit 17 with PC 1.
In the present embodiment, also comprise the LCD display 18 of joining with main control chip 2.
In the present embodiment, described main control chip 2 is ARM microprocessor.
In the present embodiment, described the first temperature sensor 11, the second temperature sensor 12 and three-temperature sensor 13 are platinum resistance thermometer sensor, PT1000.
In the present embodiment, described interface circuit 17 is USB3.0 interface.
The above; it is only preferred embodiment of the present utility model; not the utility model is imposed any restrictions; every any simple modification of above embodiment being done according to the utility model technical spirit, change and equivalent structure change, and all still belong in the protection domain of technical solutions of the utility model.
Claims (5)
1. a PCR instrument temperature control system, is characterized in that: comprise PC (1), main control chip (2), Flash storer (3), eprom memory (4), the signal isolation circuit (5) sequentially connecting, filtering and amplifying circuit (6) and A/D change-over circuit (7), the sample base being formed by base (8) and sidewall (9), be arranged on the reactive tank for holding test tubes on described sample base, directly over being arranged on described reactive tank, form the heat lid (10) of condensate water for maintaining described test tube head temperature at described test tube top to prevent test solution evaporation, for detecting in real time first temperature sensor (11) of the reactive tank temperature that is distributed in base (8) center section, for detecting in real time the second temperature sensor (12) near the reactive tank temperature of sidewall (9), for detecting in real time the three-temperature sensor (13) of heat lid (10) temperature, the first temperature control circuit (14) that the temperature of base (8) is controlled, the second temperature control circuit (15) that the temperature of oppose side wall (9) is controlled and the heater circuit (16) that heat lid (10) is heated, described the first temperature control circuit (14) includes with the second temperature control circuit (15) photoelectrical coupler, D/A change-over circuit, power amplified drive circuit and the semiconductor chilling plate that are connected successively, the quantity of described semiconductor chilling plate is multiple, and multiple described semiconductor chilling plate is mounted on respectively the bottom surface of base (8) and the surrounding of sidewall (9), described the first temperature sensor (11), the second temperature sensor (12) and three-temperature sensor (13) all join with signal isolation circuit (5), described A/D change-over circuit (7), the first temperature control circuit (14), the second temperature control circuit (15), heater circuit (16), Flash storer (3) and eprom memory (4) all join with main control chip (2), and described main control chip (2) is connected by interface circuit (17) with PC (1).
2. according to a kind of PCR instrument temperature control system claimed in claim 1, it is characterized in that: also comprise the LCD display (18) of joining with main control chip (2).
3. according to a kind of PCR instrument temperature control system described in claim 1 or 2, it is characterized in that: described main control chip (2) is ARM microprocessor.
4. according to a kind of PCR instrument temperature control system described in claim 1 or 2, it is characterized in that: described the first temperature sensor (11), the second temperature sensor (12) and three-temperature sensor (13) are platinum resistance thermometer sensor, PT1000.
5. according to a kind of PCR instrument temperature control system described in claim 1 or 2, it is characterized in that: described interface circuit (17) is USB3.0 interface.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201320713067.0U CN203616670U (en) | 2013-11-10 | 2013-11-10 | PCR instrument temperature control system |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201320713067.0U CN203616670U (en) | 2013-11-10 | 2013-11-10 | PCR instrument temperature control system |
Publications (1)
Publication Number | Publication Date |
---|---|
CN203616670U true CN203616670U (en) | 2014-05-28 |
Family
ID=50769301
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201320713067.0U Expired - Fee Related CN203616670U (en) | 2013-11-10 | 2013-11-10 | PCR instrument temperature control system |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN203616670U (en) |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104048981A (en) * | 2014-03-31 | 2014-09-17 | 北京金自天正智能控制股份有限公司 | Thermostatic control system for X ray fluorescence spectrophotometer and control method using the same |
CN104132691A (en) * | 2014-07-31 | 2014-11-05 | 国网上海市电力公司 | Operating parameter acquisition circuit of flow battery charging and discharging system |
CN104202844A (en) * | 2014-08-26 | 2014-12-10 | 无锡市华牧机械有限公司 | Heating circuit used in biomass particle machine and provided with filter circuit |
CN104382557A (en) * | 2014-11-20 | 2015-03-04 | 西安邮电大学 | Infrared spectrum analysis based cancer cell early-warning method and system |
CN104202844B (en) * | 2014-08-26 | 2016-11-30 | 重庆文润科技有限公司 | There is in biomass granulation machine the heater circuit of filter circuit |
CN110361365A (en) * | 2019-02-03 | 2019-10-22 | 北京理工大学 | A kind of scanning fluoroscopic imaging device and the portable QPCR device using it |
-
2013
- 2013-11-10 CN CN201320713067.0U patent/CN203616670U/en not_active Expired - Fee Related
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104048981A (en) * | 2014-03-31 | 2014-09-17 | 北京金自天正智能控制股份有限公司 | Thermostatic control system for X ray fluorescence spectrophotometer and control method using the same |
CN104132691A (en) * | 2014-07-31 | 2014-11-05 | 国网上海市电力公司 | Operating parameter acquisition circuit of flow battery charging and discharging system |
CN104202844A (en) * | 2014-08-26 | 2014-12-10 | 无锡市华牧机械有限公司 | Heating circuit used in biomass particle machine and provided with filter circuit |
CN104202844B (en) * | 2014-08-26 | 2016-11-30 | 重庆文润科技有限公司 | There is in biomass granulation machine the heater circuit of filter circuit |
CN104382557A (en) * | 2014-11-20 | 2015-03-04 | 西安邮电大学 | Infrared spectrum analysis based cancer cell early-warning method and system |
CN110361365A (en) * | 2019-02-03 | 2019-10-22 | 北京理工大学 | A kind of scanning fluoroscopic imaging device and the portable QPCR device using it |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN203616670U (en) | PCR instrument temperature control system | |
CN103173434A (en) | Method and device for carrying out polymerase chain reaction under constant-temperature heat source | |
CN104120077B (en) | A kind of real-time fluorescence DNA cloning instrument | |
CN206232730U (en) | The module thermal balance device of PCR gene amplification instrument | |
CN202415561U (en) | Quantitative PCR (Polymerase Chain Reaction) microfluidic control chip device | |
CN203658804U (en) | Wireless monitoring system of PCR instrument | |
CN109609606B (en) | Method for determining relative enzyme activity of DNA polymerase | |
Wan et al. | Sub-5-minute ultrafast PCR using digital microfluidics | |
CN104635801A (en) | PCR (polymerase chain reaction) instrument temperature controlling system | |
CN208505898U (en) | Microlayer model container and microlayer model generate kit | |
CN201188068Y (en) | Heating digestion apparatus | |
CN203079967U (en) | PCR (Polymerase Chain Reaction) tube | |
CN208580336U (en) | Temperature control device | |
CN103525936A (en) | Specific identification for transgenic rice kefeng No.6 strain via adoption of RPA (Recombinase Ploymerase Amplification) technology | |
TWI445819B (en) | Methods and apparatuses for convective polymerase chain reaction | |
CN104637275A (en) | PCR instrument wireless monitoring system | |
CN206161179U (en) | Multichannel digital thermometer | |
CN110069084A (en) | Temperature control device | |
CN103282496B (en) | Method for setting temperature of polymerase chain reaction and instrument thereof | |
CN104328176A (en) | Rapid direction method for nucleic acid sensor based on PCR | |
CN206440601U (en) | The temperature control device of dry type hematuria biochemics analyzer optical system heating | |
CN103757094A (en) | Fluorescent PCR based method for detecting polymorphism of TYMS gene | |
CN202390442U (en) | Heat transfer detection device based on DNA amplification | |
CN104198525B (en) | A kind of method --- method of comparison for measuring metallurgical insulation material heat insulation effect | |
CN103160594A (en) | Hydrophobic chip based multiple PCR (polymerase chain reaction) method |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140528 Termination date: 20141110 |
|
EXPY | Termination of patent right or utility model |