CN104637275A - PCR instrument wireless monitoring system - Google Patents
PCR instrument wireless monitoring system Download PDFInfo
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- CN104637275A CN104637275A CN201310560285.XA CN201310560285A CN104637275A CN 104637275 A CN104637275 A CN 104637275A CN 201310560285 A CN201310560285 A CN 201310560285A CN 104637275 A CN104637275 A CN 104637275A
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- temperature
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- pcr instrument
- temperature sensor
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P90/00—Enabling technologies with a potential contribution to greenhouse gas [GHG] emissions mitigation
- Y02P90/02—Total factory control, e.g. smart factories, flexible manufacturing systems [FMS] or integrated manufacturing systems [IMS]
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Abstract
The invention discloses a PCR instrument remote monitoring system, which includes an on-site PCR instrument temperature control system, a monitoring center upper computer and a WiFi mobile phone. The on-site PCR instrument temperature control system comprises a main control chip, a first temperature sensor, a second temperature sensor, a third temperature sensor, a first temperature control circuit, a second temperature control circuit and a heating circuit. The main control chip and the monitoring center upper computer are both connected to WiFi wireless network cards, and are both connected to the Internet for communication through the WiFi wireless network cards. The PCR instrument wireless monitoring system provided by the invention can perform wireless remote monitoring on the pedestal temperature and side wall temperature of a sample base simultaneously, and effectively solves the problems of not high accuracy, low DNA fragment amplification efficiency, high labor intensity of workers, low work efficiency and the like in existing temperature control systems.
Description
Technical field
The present invention relates to a kind of supervisory system, especially relate to a kind of PCR instrument wireless supervisory control system.
Background technology
The life science that develops into of Life Science Instruments provides effective tool and strong research means.Make the further investigation that it is developed by leaps and bounds on molecular level from the research cellular level.Leave the support of these modernization Life Science Instruments, many great research projects and engineering all will be had difficulty in taking a step.PCR instrument device be a kind ofly to be widely used, very important Life Science Instruments.PCR (polymerase chain reaction, PCR) is a kind of Enzymology method of external rapid amplifying DNA fragment specific, and the basic process that its PCR reacts is divided into three steps.The first step, DNA sex change (94 DEG C), double-stranded DNA template is hydrogen bond rupture under heat effect, forms single stranded DNA; Second step, annealing (55 DEG C), system temperature reduces, and primer is combined with DNA profiling, forms local double-strand; 3rd step, extending (72 DEG C), under the effect of Taq enzyme, is that raw material extends from 5 ends of primer to 3 ends with dNTP, the DNA chain of synthesis and template complementation.PCR instrument reaches different temperatures by Quality control exactly, carries out sex change, annealing and polymerization process to the DNA fragmentation be amplified, to reach the object amount of DNA fragmentation increased at double.Therefore, temperature controlled precision, the especially time controling of each temperature value, directly affect the efficiency of DNA fragmentation amplification.
Existing PCR instrument all only carries out temperature sampling and control to bottom sample base usually, do not consider that the sidewall of sample base and the convection heat transfer of peripheral air are on the impact of base-plate temp homogeneity, thus do not reach higher temperature-controlled precision, have impact on the efficiency of DNA fragmentation amplification.On the other hand, the human-computer interaction interface of existing PCR instrument inputs mainly through mechanical button, and LCD display translation information realizes man-machine interaction.When this just means that PCR instrument is run, staff must on instrument side, and the working time of a PCR is 2 ~ 4 hours.Therefore be difficult to realize real-time monitoring, and add labour intensity, reduce work efficiency.
Summary of the invention
Technical matters to be solved by this invention is, for above-mentioned deficiency of the prior art, to provide a kind of PCR instrument wireless supervisory control system, and work can be carried out wireless remote setting to the working procedure of PCR instrument and realize monitoring in real time to the temperature control system of PCR instrument.
For solving the problems of the technologies described above, the technical solution used in the present invention is: a kind of PCR instrument wireless supervisory control system, is characterized in that: comprise on-the-spot PCR instrument temperature control system, Surveillance center's host computer and the WIFI mobile phone with Surveillance center's host computer wireless connections, described on-the-spot PCR instrument temperature control system comprises main control chip, flash storage, eprom memory, LCD display, the signal isolation circuit sequentially connected, filtering and amplifying circuit and A/D change-over circuit, the sample base be made up of base and sidewall, be arranged on the reactive tank for holding test tubes on described sample base, be arranged on directly over described reactive tank and evaporate to prevent test solution the heat lid forming condensate water at described test tube top for maintaining described test tube head temperature, for detecting the first temperature sensor being distributed in the reactive tank temperature of base center section in real time, for detecting the second temperature sensor of the reactive tank temperature near sidewall in real time, for detecting the three-temperature sensor of hot lid temperature in real time, to the first temperature control circuit that the temperature of base controls, the temperature of oppose side wall carries out the second temperature control circuit controlled and the heater circuit heated heat lid, described first temperature control circuit includes with the second temperature control circuit the photoelectrical coupler be connected successively, D/A change-over circuit, power amplified drive circuit and semiconductor chilling plate, the quantity of described semiconductor chilling plate is multiple, and multiple described semiconductor chilling plate is mounted on the bottom surface of base and the surrounding of sidewall respectively, described first temperature sensor, second temperature sensor and three-temperature sensor all connect with signal isolation circuit, described A/D change-over circuit, first temperature control circuit, second temperature control circuit, heater circuit, flash storage, eprom memory and LCD display all connect with main control chip, described main control chip and Surveillance center's host computer are all connected with WIFI wireless network card, described main control chip and Surveillance center host computer are all connected to internet by WIFI wireless network card and communicate.
Above-mentioned a kind of PCR instrument wireless supervisory control system, is characterized in that: described Surveillance center host computer is the PC being provided with configuration software.
Above-mentioned a kind of PCR instrument wireless supervisory control system, is characterized in that: described main control chip is ARM microprocessor.
Above-mentioned a kind of PCR instrument wireless supervisory control system, is characterized in that: described first temperature sensor, the second temperature sensor and three-temperature sensor are platinum resistance thermometer sensor, PT1000.
The present invention compared with prior art has the following advantages: realtime control can be good, and monitoring interface is vividly accurate; Circuit is simply efficient, and antijamming capability is strong; Work can carry out wireless remote monitering to the base of sample base and the temperature of sidewall simultaneously, ensure that the homogeneity of sample base Temperature Distribution, the time controling that the temperature of each reactive tank of sample base and temperature are kept is accurate, efficiently solves the problems such as existing temperature control system precision is not high, the efficiency of DNA fragmentation amplification is low, labor strength large, inefficiency.
Below by drawings and Examples, technical scheme of the present invention is described in further detail.
Accompanying drawing explanation
Fig. 1 is schematic block circuit diagram of the present invention.
Description of reference numerals:
1-Surveillance center host computer; 2-main control chip; 3-flash storage;
4-eprom memory; 5-signal isolation circuit; 6-filtering and amplifying circuit;
7-A/D change-over circuit; 8-base; 9-sidewall;
10-heat lid; 11-the first temperature sensor; 12-the second temperature sensor;
13-the three-temperature sensor; 14-the first temperature control circuit; 15-the second temperature control circuit;
16-heater circuit; 17-LCD display; 18-WIFI mobile phone.
19-WIFI wireless network card; 20-internet.
Embodiment
As shown in Figure 1, the present invention includes on-the-spot PCR instrument temperature control system, Surveillance center's host computer 1 and the WIFI mobile phone 18 with Surveillance center's host computer 1 wireless connections, described on-the-spot PCR instrument temperature control system comprises main control chip 2, flash storage 3, eprom memory 4, LCD display 17, the signal isolation circuit 5 sequentially connected, filtering and amplifying circuit 6 and A/D change-over circuit 7, the sample base be made up of base 8 and sidewall 9, be arranged on the reactive tank for holding test tubes on described sample base, be arranged on directly over described reactive tank and evaporate to prevent test solution the heat lid 10 forming condensate water at described test tube top for maintaining described test tube head temperature, for detecting the first temperature sensor 11 being distributed in the reactive tank temperature of base 8 center section in real time, for detecting the second temperature sensor 12 of the reactive tank temperature near sidewall 9 in real time, for detecting the three-temperature sensor 13 of heat lid 10 temperature in real time, to the first temperature control circuit 14 that the temperature of base 8 controls, the temperature of oppose side wall 9 carries out the second temperature control circuit 15 controlled and the heater circuit 16 heated heat lid 10, described first temperature control circuit 14 includes with the second temperature control circuit 15 photoelectrical coupler be connected successively, D/A change-over circuit, power amplified drive circuit and semiconductor chilling plate, the quantity of described semiconductor chilling plate is multiple, and multiple described semiconductor chilling plate is mounted on the bottom surface of base 8 and the surrounding of sidewall 9 respectively, described first temperature sensor 11, second temperature sensor 12 and three-temperature sensor 13 all connect with signal isolation circuit 5, described A/D change-over circuit 7, first temperature control circuit 14, second temperature control circuit 15, heater circuit 16, flash storage 3, eprom memory 4 and LCD display 17 all connect with main control chip 2, described main control chip 2 and Surveillance center's host computer 1 are all connected with WIFI wireless network card 19, described main control chip 2 is all connected to internet 20 by WIFI wireless network card 19 with Surveillance center host computer 1 and communicates.
In the present embodiment, described Surveillance center host computer 1 is for being provided with the PC of configuration software.
In the present embodiment, described main control chip 2 is ARM microprocessor.
In the present embodiment, described first temperature sensor 11, second temperature sensor 12 and three-temperature sensor 13 are platinum resistance thermometer sensor, PT1000.
The above; it is only preferred embodiment of the present invention; not the present invention is imposed any restrictions, every above embodiment is done according to the technology of the present invention essence any simple modification, change and equivalent structure change, all still belong in the protection domain of technical solution of the present invention.
Claims (4)
1. a PCR instrument wireless supervisory control system, is characterized in that: comprise on-the-spot PCR instrument temperature control system, Surveillance center's host computer (1) and the WIFI mobile phone (18) with Surveillance center's host computer (1) wireless connections, described on-the-spot PCR instrument temperature control system comprises main control chip (2), flash storage (3), eprom memory (4), LCD display (17), the signal isolation circuit (5) sequentially connected, filtering and amplifying circuit (6) and A/D change-over circuit (7), the sample base be made up of base (8) and sidewall (9), be arranged on the reactive tank for holding test tubes on described sample base, be arranged on directly over described reactive tank and evaporate to prevent test solution the heat lid (10) forming condensate water at described test tube top for maintaining described test tube head temperature, for detecting the first temperature sensor (11) being distributed in the reactive tank temperature of base (8) center section in real time, for detecting second temperature sensor (12) of the reactive tank temperature near sidewall (9) in real time, for detecting the three-temperature sensor (13) of heat lid (10) temperature in real time, to the first temperature control circuit (14) that the temperature of base (8) controls, the temperature of oppose side wall (9) carries out the second temperature control circuit (15) controlled and the heater circuit (16) heated heat lid (10), described first temperature control circuit (14) includes with the second temperature control circuit (15) photoelectrical coupler be connected successively, D/A change-over circuit, power amplified drive circuit and semiconductor chilling plate, the quantity of described semiconductor chilling plate is multiple, and multiple described semiconductor chilling plate is mounted on the bottom surface of base (8) and the surrounding of sidewall (9) respectively, described first temperature sensor (11), second temperature sensor (12) and three-temperature sensor (13) all connect with signal isolation circuit (5), described A/D change-over circuit (7), first temperature control circuit (14), second temperature control circuit (15), heater circuit (16), flash storage (3), eprom memory (4) and LCD display (17) all connect with main control chip (2), described main control chip (2) and Surveillance center's host computer (1) are all connected with WIFI wireless network card (19), described main control chip (2) and Surveillance center's host computer (1) are all connected to internet (20) by WIFI wireless network card (19) and communicate.
2. according to a kind of PCR instrument wireless supervisory control system according to claim 1, it is characterized in that: described Surveillance center host computer (1) is for being provided with the PC of configuration software.
3. according to a kind of PCR instrument wireless supervisory control system described in claim 1 or 2, it is characterized in that: described main control chip (2) is ARM microprocessor.
4. according to a kind of PCR instrument wireless supervisory control system described in claim 1 or 2, it is characterized in that: described first temperature sensor (11), the second temperature sensor (12) and three-temperature sensor (13) are platinum resistance thermometer sensor, PT1000.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310560285.XA CN104637275A (en) | 2013-11-10 | 2013-11-10 | PCR instrument wireless monitoring system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310560285.XA CN104637275A (en) | 2013-11-10 | 2013-11-10 | PCR instrument wireless monitoring system |
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| Publication Number | Publication Date |
|---|---|
| CN104637275A true CN104637275A (en) | 2015-05-20 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201310560285.XA Pending CN104637275A (en) | 2013-11-10 | 2013-11-10 | PCR instrument wireless monitoring system |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110554651A (en) * | 2019-09-19 | 2019-12-10 | 哈尔滨工业大学 | Private Internet of things system for measuring and controlling temperature of microfluidic chip |
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- 2013-11-10 CN CN201310560285.XA patent/CN104637275A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110554651A (en) * | 2019-09-19 | 2019-12-10 | 哈尔滨工业大学 | Private Internet of things system for measuring and controlling temperature of microfluidic chip |
| CN110554651B (en) * | 2019-09-19 | 2021-07-30 | 哈尔滨工业大学 | Private Internet of things system for measuring and controlling temperature of microfluidic chip |
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Application publication date: 20150520 |