CN203079967U - PCR (Polymerase Chain Reaction) tube - Google Patents
PCR (Polymerase Chain Reaction) tube Download PDFInfo
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- CN203079967U CN203079967U CN 201320045502 CN201320045502U CN203079967U CN 203079967 U CN203079967 U CN 203079967U CN 201320045502 CN201320045502 CN 201320045502 CN 201320045502 U CN201320045502 U CN 201320045502U CN 203079967 U CN203079967 U CN 203079967U
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- tube
- pcr
- pcr reaction
- reaction tubes
- pipe close
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Abstract
The utility model relates to a PCR (Polymerase Chain Reaction) tube comprising a tube body and a tube plug, wherein the tube body is made of a metal material; the outer wall at the tube mouth is provided with a plastic layer; and at least two fastening parts for fixing the tube plug are uniformly arranged on the plastic layer. According to the utility model, a traditional PCR tube formed through injection molding of a plastic material is changed and the metal material with strong thermal conductivity is utilized as the raw material of the PCR tube, so that the temperature of a reaction liquid in the PCR tube and the temperature of a heating system are synchronously changed and the reaction time of the PCR is shortened; and the fastening parts for fixing the tube plug are arranged on the plastic layer at the outer wall of the tube mouth, so that the tube body and the tube plug are firmly connected and closely cooperated, the tube plug is incapable of spattering and polluting other samples under the influence of external conditions and being polluted by other matters, the sealing effect of the PCR tube is increased and the accuracy of the reaction result is guaranteed.
Description
Technical field
The utility model relates to a kind of laboratory apparatus of biological technical field, particularly a kind of PCR reaction tubes in PCR experiment field.
Background technology
At present, in biology field, the PCR reaction is the experimental technique of using always, the PCR reaction is that (Polymerase Chain Reaction PCR), is a kind of method of the synthetic specific DNA fragment of external enzymatic in the polymerase chain reaction, form one-period by several steps reactions such as high-temperature denatured, low-temperature annealing and thermophilic extension, circulation is carried out, and makes target DNA be able to rapid amplification, have high specificity, highly sensitive, easy and simple to handle, characteristics such as save time.It not only can be used for fundamental researchs such as gene isolation, clone and nucleic acid sequence analysis, also can be used for the diagnosis of disease.Round pcr claims cell-free molecular cloning or the directed enzymatic amplification technique of the external primer of specific DNA sequences again.
The PCR reaction tubes is a reaction vessels that generally uses during pcr amplification reaction is implemented, a typical pcr amplification reaction is by the PCR thermal cycler heating and cooling that the PCR reaction tubes that is placed on wherein carries out repeatedly to be finished, the conduction of temperature is to pass to the PCR reaction tubes that is placed on wherein again by PCR thermal cycler heatable metal block in this process, tube wall through the PCR reaction tubes conducts heat to the interior PCR reaction solution of pipe then, thereby causes amplified reaction.Whether a PCR reaction can rapidly and efficiently increase except the composition of gene module quality, polysaccharase character, reaction solution to the gene module, and the speed of the heating and cooling of reaction solution also is key factor.The efficient of the fast more PCR reaction of warming and cooling rate is just high more, and with regard to the non-purpose amplified production of difficult more generation, and warming and cooling rate is fast, can shorten the time of PCR reflection greatly.Though and the used PP material thermal conductivity of traditional PCR reaction tubes better (thermal conductivity about 0.2), the temperature of PCR reaction tubes internal reaction liquid is difficult to the temperature of heating system synchronously in the heat-processed repeatedly of experiment, has the 5-10 hysteresis of second.When using traditional PCR reaction tubes to experimentize the chamber, the time of single experiment needs two to three hours.In addition, PCR is extremely fine reaction, if the careless manipulation sample may splash out, causes the inaccurate of sample, also has other subtitle substance and enters in the pipe, influences the accuracy of experimental result.
At above-mentioned deficiency, a kind of novel PCR reaction tubes need be provided, when heating up or lower the temperature, the temperature of PCR reaction tubes internal reaction liquid and the temperature of heating unit are consistent as much as possible, improve the heat transfer rate of PCR reaction tubes; Also need increase the obturation effect of PCR reaction tubes, guarantee the accuracy of reaction result.
The utility model content
In view of this, the utility model provides a kind of PCR reaction tubes, and its heat-transfer effect is good, and good leak tightness has reduced the time that PCR reacts, and has guaranteed the accuracy of experimental result.
The utility model solves the problems of the technologies described above by following technique means: a kind of PCR reaction tubes, comprise body and pipe close, described body is made by metallic substance, and the outer wall at mouth of pipe place is provided with plastic layer, evenly is provided with at least two fixedly locked parts of pipe close on the described plastic layer.
Further, described pipe close is for inserting the interior plug of body, and the center has an axial column-shaped projection, and the diameter of described column-shaped projection is identical with the internal diameter of the mouth of pipe.
Further, described locked part is a clip, and described pipe close is provided with the buckle of corresponding clip.
Further, described body lower end is taper, and its bottom is a cambered surface.
Further, described metallic substance is copper, aluminium or alloy material.
Further, described body is connected by flexible frenulum with pipe close.
Further, the thickness of described body is 0.05-0.15mm.
Further, described pipe close is made by polypropylene material.
The beneficial effects of the utility model: PCR reaction tubes of the present utility model, comprise body and pipe close, described body is made by metallic substance, and the outer wall at mouth of pipe place is provided with plastic layer, evenly is provided with at least two fixedly locked parts of pipe close on the described plastic layer.The utility model has changed the injection molded PCR reaction tubes of material of the plastic nature of traditional employing, utilization has the metallic substance of strong thermal conductivity as the raw material of making the PCR reaction tubes, the temperature of the reaction solution in the PCR reaction tubes and the temperature of heating system are changed synchronously, shortened the time of PCR reaction.The plastic layer of mouth of pipe outer wall is provided with the fixedly locked part of pipe close, body and pipe close are connected firmly, cooperate closely, pipe close can not be subjected to the influence of ambient conditions and spill the sample that pollutes other, be not subject to the pollution of other material yet, increase the obturation effect of PCR reaction tubes, guaranteed the accuracy of reaction result.
Description of drawings
Below in conjunction with drawings and Examples the utility model is further described.
Fig. 1 is a structural representation of the present utility model.
The PCR electrophoretogram of gained when Fig. 2 carries out biological experiment for using the utility model product.
Embodiment
Below with reference to accompanying drawing the utility model is elaborated, as shown in the figure: the PCR reaction tubes of present embodiment, comprise body 1 and pipe close 2, described body 1 is made by metallic substance, the outer wall at mouth of pipe place is provided with plastic layer 3, evenly is provided with at least two fixedly locked parts of pipe close 2 on the described plastic layer 3.Utilization has the metallic substance of strong thermal conductivity as the raw material of making the PCR reaction tubes, and the temperature of PCR reaction tubes internal reaction liquid and the temperature of heating system are changed synchronously, has shortened the time of PCR reaction; Mouth of pipe outer wall is provided with plastic layer 3, is convenient to gripping PCR reaction tubes, also is convenient at the mouth of pipe the fixedly locked part of pipe close is set; The setting of locked part is connected firmly body 1 and pipe close 2, cooperate closely, managing intravital reaction solution can not be subjected to the influence of ambient conditions and eject the sample that pollutes other, some subtitle substances can not enter in the body yet, increase the obturation effect of PCR reaction tubes, guaranteed the accuracy of reaction result.
As further improvement in the technical proposal, described pipe close 2 is for inserting the interior plug of body, and there is an axial column-shaped projection 6 at its center, and the diameter of described column-shaped projection 6 is identical with the internal diameter of the mouth of pipe.Axial column-shaped projection 6 is set on the pipe close, and its diameter is identical with the internal diameter of the mouth of pipe, and the form of plug is inserted body in adopting, and can increase airtight effect, also can make things convenient for the unlatching of pipe close.
As further improvement in the technical proposal, described locked part 4 is a clip, and described pipe close 2 is provided with the buckle 5 of corresponding clip.With the mounting block of buckle as body 1 and pipe close 2, the convenient setting, and body 1 is easy to separate with pipe close 2.
As further improvement in the technical proposal, described body 1 lower end is taper, and its bottom is a cambered surface.The cambered surface setting of the taper of body lower end and bottom makes and is difficult for residual reaction solution in the PCR reaction tubes, and it is convenient to get liquid.
As further improvement in the technical proposal, described metallic substance is copper, aluminium or alloy material.Copper, aluminium or alloy material are drawn materials conveniently, and its heat conductivility is good, can shorten the PCR reaction times greatly.
As further improvement in the technical proposal, described body 1 is connected by flexible frenulum 7 with pipe close 2.Body 1 is connected by flexible strap 7 with pipe close 2, and frenulum can bend arbitrarily, can cover the PCR reaction tubes after the sampling, avoids the pollution of external substance.
As further improvement in the technical proposal, the thickness of described body 1 is 0.05-0.15mm.Body thickness only is 0.05-0.15mm, and is preferred, is made as 0.1mm, and the temperature of PCR reaction tubes internal reaction liquid and the temperature of heating system are changed synchronously, reduces the time of PCR reaction.
As further improvement in the technical proposal, described pipe close 2 is made by polypropylene material.Polypropylene material character is more stable, and has certain transparency, can see through the state that pipe close is observed body internal reaction liquid.
The applicant is after having used product of the present utility model, obtained good experiment effect, below illustrate with specific examples: the applicant has used aluminum PCR reaction tubes identifying in the experiment of morel with molecular assay method, and the ITS region sequence has been carried out the pcr amplification experiment.Test as follows:
Get fungi universal primer ITS1 (5 '-TCCGTAGGTGAACCTGCGC-3 ') and ITS4 (5 '-TCCTCCGCTTATTGATATGC-3 ') (synthetic), carry out the amplification of ITS region sequence by the English Weihe River, Shanghai Jie Ji Bioisystech Co., Ltd.25 μ l PCR reaction: 1 * PCR damping fluid, 0.625U Taq enzyme, 1.5mM MgCl2,0.2mM dNTPs, 0.4 μ M primer ITS1,0.4 μ M primerITS4,50-100ng dna profiling diluent.Increase from strains tested extracting dna profiling with PCR method and to have obtained the rDNA-ITS section of long 1200bp, its PCR electrophoretogram such as Fig. 2.Use traditional PCR reaction tubes to carry out the experimental arrangement and the required time of PCR reaction: 94 ℃ of 2min; 94 ℃ of 30s, 59 ℃ of 30s, 72 ℃ of 90s, 35Cycles; 72 ℃ of 7min.
Hence one can see that, and only the experiment of the pcr amplification of this individual event then needs nearly 100min.Use aluminum PCR reaction tubes of the present utility model carry out this experiment, the time of each working cycle all can contract to 1/2, has reduced experimental period greatly, and the proof metallic substance is very little to the absorption of reaction solution, the obturation effect of body is good, has satisfied the requirement of experiment of expection.
Explanation is at last, above embodiment is only unrestricted in order to the explanation the technical solution of the utility model, although the utility model is had been described in detail with reference to preferred embodiment, those of ordinary skill in the art is to be understood that, can make amendment or be equal to replacement the technical solution of the utility model, and not breaking away from the aim and the scope of technical solutions of the utility model, it all should be encompassed in the middle of the claim scope of the present utility model.
Claims (8)
1. PCR reaction tubes, comprise body (1) and pipe close (2), it is characterized in that: described body (1) is made by metallic substance, and the outer wall at mouth of pipe place is provided with plastic layer (3), evenly is provided with at least two fixedly locked parts (4) of pipe close (2) on the described plastic layer (3).
2. PCR reaction tubes according to claim 1 is characterized in that: described pipe close (2) is for inserting the interior plug of body, and there is an axial column-shaped projection (6) at its center, and the diameter of described column-shaped projection (6) is identical with the internal diameter of the mouth of pipe.
3. PCR reaction tubes according to claim 1 is characterized in that: described locked part (4) is a clip, and described pipe close (2) is provided with the buckle (5) of corresponding clip.
4. PCR reaction tubes according to claim 1 is characterized in that: described body (1) lower end is taper, and its bottom is a cambered surface.
5. according to each described PCR reaction tubes of claim 1-4, it is characterized in that: described metallic substance is copper, aluminium or alloy material.
6. PCR reaction tubes according to claim 5 is characterized in that: described body (1) is connected by flexible frenulum (7) with pipe close (2).
7. PCR reaction tubes according to claim 6 is characterized in that: the thickness of described body (1) is 0.05-0.15mm.
8. PCR reaction tubes according to claim 7 is characterized in that: described pipe close (2) is made by polypropylene material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320045502 CN203079967U (en) | 2013-01-28 | 2013-01-28 | PCR (Polymerase Chain Reaction) tube |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320045502 CN203079967U (en) | 2013-01-28 | 2013-01-28 | PCR (Polymerase Chain Reaction) tube |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203079967U true CN203079967U (en) | 2013-07-24 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201320045502 Expired - Fee Related CN203079967U (en) | 2013-01-28 | 2013-01-28 | PCR (Polymerase Chain Reaction) tube |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203079967U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106701560A (en) * | 2016-06-13 | 2017-05-24 | 秦荣 | PCR test tube |
| CN106755457A (en) * | 2017-01-09 | 2017-05-31 | 杭州安杰思生物科技有限公司 | A kind of PCR detection method of use metal test tubes |
| CN106867892A (en) * | 2017-01-09 | 2017-06-20 | 杭州安杰思生物科技有限公司 | A kind of metal test tubes PCR instrument |
| CN109735437A (en) * | 2019-01-28 | 2019-05-10 | 长春长光辰英生物科学仪器有限公司 | It is collected and the vessel and method that handle after a kind of ejection sorting of cell for cell |
-
2013
- 2013-01-28 CN CN 201320045502 patent/CN203079967U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106701560A (en) * | 2016-06-13 | 2017-05-24 | 秦荣 | PCR test tube |
| CN106755457A (en) * | 2017-01-09 | 2017-05-31 | 杭州安杰思生物科技有限公司 | A kind of PCR detection method of use metal test tubes |
| CN106867892A (en) * | 2017-01-09 | 2017-06-20 | 杭州安杰思生物科技有限公司 | A kind of metal test tubes PCR instrument |
| CN109735437A (en) * | 2019-01-28 | 2019-05-10 | 长春长光辰英生物科学仪器有限公司 | It is collected and the vessel and method that handle after a kind of ejection sorting of cell for cell |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130724 Termination date: 20210128 |
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| CF01 | Termination of patent right due to non-payment of annual fee |